KR20100041605A - Method of culturing ganoderma lucidum karst - Google Patents

Method of culturing ganoderma lucidum karst Download PDF

Info

Publication number
KR20100041605A
KR20100041605A KR1020080100865A KR20080100865A KR20100041605A KR 20100041605 A KR20100041605 A KR 20100041605A KR 1020080100865 A KR1020080100865 A KR 1020080100865A KR 20080100865 A KR20080100865 A KR 20080100865A KR 20100041605 A KR20100041605 A KR 20100041605A
Authority
KR
South Korea
Prior art keywords
grafting
ganoderma lucidum
mushrooms
days
mushroom
Prior art date
Application number
KR1020080100865A
Other languages
Korean (ko)
Other versions
KR101006734B1 (en
Inventor
차준돈
문상영
김선우
Original Assignee
차준돈
김선우
문상영
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 차준돈, 김선우, 문상영 filed Critical 차준돈
Priority to KR1020080100865A priority Critical patent/KR101006734B1/en
Publication of KR20100041605A publication Critical patent/KR20100041605A/en
Application granted granted Critical
Publication of KR101006734B1 publication Critical patent/KR101006734B1/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/40Cultivation of spawn
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/64Cultivation containers; Lids therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Mushroom Cultivation (AREA)

Abstract

PURPOSE: A method of manufacturing ornamental Ganoderma lucidum using a grafting unit is provided to improve productivity of products by cultivating different shapes of stems and caps of Ganoderma lucidum through grafting of several mushrooms upon Ganoderma lucidum. CONSTITUTION: A method of manufacturing ornamental Ganoderma lucidum comprises the following steps: sterilizing log(1) having the average diameter of 15~20cm, a length of 20cm and a moisture content of 42 ~ 45 weight% by surrounding the log with a heat resistance polyethylene tube; inoculating spawn(4) on a log medium; grafting scion mushroom with a grafting unit; rooting the mushroom in 24~38°C for 3 ~ 5 days; and processing products by attaching the mushrooms on rootstock such as Mokbujak charcoal and Seokbujak charcoal.

Description

접 붙이기 수단을 이용한 관상용 영지버섯 제조방법{METHOD OF CULTURING GANODERMA LUCIDUM KARST}Method for preparing ornamental ganoderma lucidum mushroom using gluing means {METHOD OF CULTURING GANODERMA LUCIDUM KARST}

본 발명은 영지버섯을 관상용 상품으로 만들기 위한 제조방법에 관한 것으로 보다 구체적인 것은 영지버섯에 여러개의 버섯을 접붙여서 버섯줄기와 맛의 모양이 다양하도록 키워 관상용 상품이 되게 한 것이다.The present invention relates to a manufacturing method for making ganoderma lucidum mushroom as an ornamental product, and more specifically, to cultivate a variety of mushroom stems and tastes by grafting several mushrooms on ganoderma lucidum mushroom to become an ornamental product.

영지버섯(Ganoderma lucidum)은 활엽수 고사목과 그루터기에 자생하는 목재부후균으로 북반구 온대 이북에 광범위하게 분포하고 있으며, 자실체는 예로부터 한국, 중국, 일본등지에서 귀한 약제로 이용되어 왔다.Ganoderma lucidum is a woody fungus native to broad-leaved dead trees and stumps, and is widely distributed in the northern hemisphere temperate regions. Fruiting bodies have been used as valuable drugs in Korea, China, and Japan since ancient times.

갓과 색택에 의해 적지(赤芝), 흑지(黑芝), 황지(黃芝), 자지(紫芝), 청지(靑芝), 백지(白芝)의 6종으로 분류하고 있으나 근래에 와서는 분류학적으로 60여종이 있다고 발표되었다. 영지버섯중 적지(赤芝)의 모양은 줄기와 갓의 표면에 광택이 있는 1년생의 버섯으로 분류학상 자루곰팡이류 원숭이안장 버섯과에 속하는 버섯으로 갓은 보통 바퀴무늬가 있는 말굽형이나 타원형이다.It is classified into six kinds of red land, black ground, yellow ground, red ground, purple, blue, and white paper by gad and color selection. It has been reported that there are about 60 species. Among the ganoderma lucidum mushrooms, the shape of the red earth is a year-old mushroom with a gloss on the surface of the stem and the lampshade. It is a mushroom belonging to the taxa fungus monkey saddle mushroom family.

갓의 표면은 처음에 황백색을 띠고 있으나 생장하면 절각색~자갈색으로 먼저 자란 부분부터 색택이 변하여 간다. 갓의 뒷면은 황백색을 띄고 구멍이 많으며 줄기는 갓의 표면과 같은 색으로 약간 굴곡이 생긴다. 영지버섯은 활엽수를 기주로 하며 6월~9월의 고온기에 활엽수 그루터기 부위에서 발생한다. 또한 영지버섯은 각종 성분을 함유하고 있는데, 다당체 및 다당체 단백질 결합체와 쓴맛을 내는 테르페노이드(terpendid)계통의 물질이 주요 약효성분이며 고혈압, 면역활성, 항암활성에대한 효과가 보고된 바 있다.The surface of the lampshade is yellowish white at first, but when it grows, the color gradation changes from the first part that grows to kerosene to purple. The back of the gat is yellowish white with many holes and the stem is slightly curved with the same color as the surface of the gat. Ganoderma lucidum is based on hardwoods and occurs in the hardwood stumps during the high temperatures of June to September. In addition, Ganoderma lucidum contains a variety of ingredients, polysaccharides and polysaccharide protein conjugates and bitter terteroids (terpendid) of the substance is the main active ingredient and has been reported to have an effect on hypertension, immune activity, anticancer activity.

인공재배법이 개발되기 전에는 그 양이 극히 제한되어 널리 보급되지 못하였으나 1980년대 인공재배법이 개발되면서 새로운 농가 소득작물로 부각되어 전업 또는 부업형태로 생산되고 있다.Before the artificial cultivation method was developed, the quantity was extremely limited and it was not widely distributed. However, as the artificial cultivation method was developed in the 1980s, it is emerging as a new farm income crop and is produced as a full-time or side job.

한편, 영지버섯은 약리성이 매우 뛰어나 옛날부터 한방에서 이용되어 왔으나 자연산채취량이 많지 않아 주로 한방 약제로 이용되어 왔으며 인공재배기술이 보급됨에 따라 다양한 상품화가 시도되고 있다.On the other hand, Ganoderma lucidum mushroom is very pharmacological and has been used in traditional medicine since ancient times, but it is mainly used as a herbal medicine because there is not a lot of natural extraction and various commercialization techniques have been attempted as the artificial culture technology is spread.

본 발명은 영지버섯의 약리적 특성에 따른 선호도와 관상학적 형태와 색상을 활용하여 보다 부가가치가 높은 상품화를 위한 것으로 접목기술을 이용한 관상용 영지를 재배하여 부가가치가 높은 새로운 소득원을 개발하고자 한다.The present invention is to commercialize higher value-added by using preferences and coronary forms and colors according to the pharmacological characteristics of Ganoderma lucidum mushroom, and to develop new income sources with high added value by cultivating ornamental ganoderma using grafting technology.

본 발명은 영지버섯을 화분에 심어 관상용 상품으로 개발하는 것이다.The present invention is to develop ganoderma lucidum mushrooms in ornamental products.

본 발명은 버섯의 균사생장 특성을 접목에 이용하여 관상용 영지버섯상품화를 꾀함으로써 영지버섯이 갖고 있는 관상학적 가치를 살려 새로운 상품으로 개발한 것이다.The present invention has been developed as a new product utilizing the coronary value of Ganoderma lucidum mushrooms by commercializing the ornamental Ganoderma lucidum mushroom by using the mycelial growth characteristics of mushrooms.

본 발명에 의한 관상용 영지버섯은 접목에 의한 육성으로 영지버섯 줄기와 갓의 모양을 다양하게 육성할 수 있으므로 관상가치가 충분한 상품을 만들어 재배농가의 소득증대에 기여할 수 있는 이점이 있고 변질이 없으므로 지속적인 관상가치가 큰 장점이 있다.Ornamental ganoderma lucidum mushroom according to the present invention can cultivate a variety of shapes of ganoderma lucidum stem and gat by cultivation by grafting, there is an advantage that can contribute to the income increase of cultivation farmers by making a sufficient ornamental value and there is no alteration There is a big advantage to it.

본 발명은 참나무를 소재로 한 원목배지에 종균을 접종하여 버섯갓의 크기가 5~50cm 범위로 육성되면 대목으로 하고 별도로 육성한 접수버섯을 선택하여 갓의 크기가 2~5cm 되었을 때 선택하여 접을 붙여 육성시키는 것이다.The present invention inoculates the seedlings in the oak-based solid wood medium, when the size of the mushroom shade is grown in the range of 5 to 50 cm, the large sized and selected separately grown mushrooms when the size of the shade is 2 to 5 cm It is to nurture.

실시례Example (1)(One)

1. 재료 및 종균1. Material and spawn

가. 종균end. Spawn

관상용 상품화에 사용할 종균은 영지1호와 녹각영지(90002호)로 경기도 광주 버섯시험장에서 분양받아 사용하였다.The spawns used for ornamental commercialization were Youngji No. 1 and Nokgakji Territories (90002), which were sold at the Gwangju Mushroom Test Center.

나. 원균배양I. Probiotic culture

접종균 배양은 2007년 2월 12일부터 3월14일(31일)까지 전라남도 장흥군 장응버섯종균분양센터내 실험실에서 배양하였다.Inoculation cultures were cultured in a laboratory in Jangung Mushroom Species Culture Center in Jangheung-gun, Jeollanam-do from February 12 to March 14 (31).

접종원 조제는 분양 받은 영지1호나 녹각영지를 PDA 사면배지에서 25℃로 7일 배양하여 원균으로 사용하였고, 대량증식을 하기 위해 표1의 MCM배지에서 1회 표2의 혼합배지에서 2회 배양하여 최종량이 400L가 되도록 배양하였다.Inoculum preparation was used as a prokaryotic incubated 7 days at 25 ℃ in the PDA slope medium medium for pre-prepared for the first time, or two times in the mixed medium of Table 2 once in MCM medium of Table 1 for mass growth The final amount was incubated to 400L.

MCM배지는 500㎖ 플라스크에 150㎖ 조제 5일간 배양하였고, 혼합배지에서 증식은 2회하였는데 1차는 2L, 10일에 배양하고, 그것을 400L에 옮겨 13일 배양 접종원(액체종균)으로 사용하였다MCM medium was incubated in 500 ml flask for 5 days, 150 ml preparation, and growth was repeated twice in mixed medium. The primary culture was 2 L, 10 days, and it was transferred to 400 L and used as a 13-day inoculum (liquid seed).

Figure 112008071597911-PAT00001
Figure 112008071597911-PAT00001

다. 재배사 시설All. Planter facility

재배사 골조는 직경이 33mm인 원형파이프를 사용하여 양쪽 측면은 높이가 130cm, 중앙부위는 250cm가 되게 골조를 세우고 칸막이는 70cm 유지되게 시설한다.The planter's frame is constructed with a circular pipe of 33mm in diameter, and the frame is erected so that the sides are 130cm in height and the central part is 250cm, and the partition is maintained at 70cm.

재배사 보온재는 제일 먼저 0.04mm 두께의 폴리에틸렌필름을 덮고 난 뒤 그 위에 캐시밀론 224g(2.2×27m)이불을 2~3겹 덮고 다시 0.04mm 두께의 폴리에틸렌필름을 덮은 다음 차광률이 70%인 차광막을 덮는다.The grower insulation is first covered with 0.04mm thick polyethylene film, 2 ~ 3 layers of 224g (2.2 × 27m) quilt on cashmere, and then covered with a 0.04mm thick polyethylene film, followed by a light blocking film having a 70% shading ratio. .

라. 원목la. Solid wood

영지재배에 알맞은 참나무를 수분함량 42~45중량%, 직경 15~20cm, 길이 20cm로 잘라 사용하였다.Oak tree suitable for manor cultivation was cut into water content 42-45% by weight, diameter 15-20cm, length 20cm.

마. 원목 배지 제조hemp. Wood badge manufacturing

절단된 원목(1)을 두겹의 내열성 폴리에틸렌필름(2)에 넣어 폴리에틸렌필름의 개방부(2a)를 모아서 통기성마개(3)를 닫아 준비한다.The cut wood (1) is put into two layers of heat-resistant polyethylene film (2) to collect the open portion (2a) of the polyethylene film is prepared by closing the breathable stopper (3).

바. 원목의 살균bar. Sterilization of Logs

살균실에 넣고 증기를 공급하여 121℃를 90분간 유지시켜 증기살균한다.It is put into a sterilization chamber and steam is supplied by maintaining steam at 121 ℃ for 90 minutes.

사. 종균접종 및 배양four. Spawn inoculation and culture

살균이 끝난 원목배지는 종균(4)을 접종하기 전에 반드시 18~20℃로 유지하고 무균실에서 접종한다.Sterilized log should be maintained at 18 ~ 20 ℃ before inoculating spawn (4) and inoculated in clean room.

접종방법은 원목배지 1개당 lO∼20g 씩 접종하는데 접종된 종균이 단면 상단 에 고루 퍼지도록 하는 것이 균사생장이 빠르다. 접종이 완료 된 것은 재배사에 옮겨 배양한다.The inoculation method is inoculated with lO-20g per one wood medium, and it is faster for the mycelial growth to inoculate the seeded seeds evenly on the top of the cross section. After inoculation is completed, transfer to the grower and incubate.

재배사내의 온도는 전기히터를 사용하여 20∼25℃를 유지한다.The temperature in the grower is maintained at 20 to 25 ℃ using an electric heater.

2. 2. 접목붙이기Grafting

가. 접목방법별 접목end. Grafting by Grafting Method

접목방법이 분화 수형 구성에 미치는 영향을 알아보기 위하여 대목과 접수를 V자접과 평접을 하였다.In order to examine the effect of grafting method on the composition of differentiation tree, tree and reception were paralleled with V-shaped.

나. 접수의 시기별 접목 I. Grafting according to time of reception

영지는 발이 후 적정 접목 시기를 규명하기 위하여 갓의 크기 별로 5cm,6cm,7cm, 8cm, 9cm,10cm,6단계로 접목을 실시 하였다.Ganoderma lucidum was grafted in 5cm, 6cm, 7cm, 8cm, 9cm, 10cm, and 6 stages to determine proper grafting time after footing.

다. 접수의 길이별 접목All. Grafting by length of reception desk

접수의 길이가 접목 수형 구성에 미치는 영향을 파악하기 위하여 대의 길이별 접수를 2∼5cm 사이에서 수형구성에 따라 선택하였다.In order to understand the effect of the length of the reception on the grafting male configuration, the reception was selected according to the male configuration between 2 and 5 cm.

라. 접목 후 활착환경la. Active environment after grafting

접목 후 접목 부위의 활착에 미치는 영향을 규명하기 위해 접수와 동일한 관리, 어두운 실내조건,필름봉지 밀봉처리 실내 3단계로 하였다.In order to investigate the effect of grafting on grafting after grafting, three steps were taken in the same control, dark room conditions, and film bag sealing room.

마. 분화수형 구성hemp. Eruption type composition

영지 접목시 접목 가능 단수를 알아보기 위하여 1개의 대목을 사용하여 1 단, 2단, 3단,4단 동시 접목을 실시하였고, 단 수형은 10단까지되도록 접목을 실시하였다.In order to find out the number of possible grafts, the grafting of 1, 2, 3, and 4 stages was carried out using one part, and the number of stages was grafted up to 10 steps.

바. 분화상품화bar. Differentiation

접목이 완료 되었을 때 화분, 목부작, 석부작, 숯 등 구성에 어울리는 소재를 이용하여 분화를 구성하였다. When the grafting was completed, differentiation was composed using materials suitable for the composition such as flower pots, woodwork, stonework, and charcoal.

접목 영지는 건조한 실내에서 건조하여 표면에 니스칠을 하여 상품화 한다.Grafted ganoderma lucidum is dried in a dry room and varnished on the surface for commercialization.

실시례(2)Example (2)

1. 접목 영지의 대목선별1. Big tree selection of grafted territory

영지 접목시 대목을 선발하기 위해 녹각영지와 일반영지 두 품종 중 보다 좋은 대목을 선발하고자 생육상황을 표3과 같이 조사하였다. 생육생장 유도 30일 후 대목으로 사용 하였다.In order to select larger trees among the two varieties of nocturnal and general estates, the growth situation was examined as shown in Table 3 to select large trees when grafting the territories. 30 days after growth induction was used as a large tree.

영지 접목용 대목은 일반영지나 녹각영지 모두 대목으로 사용 가능하였으나 버섯의 원기형성 기간이 일반영지의 10일, 녹각영지는 20일 소요되었다.For the grafting of ganoderma lucidum, both normal and nocturnal ganoderma were used as large trees, but the period of priming of mushrooms took 10 days and 20 days for greenery.

적정 대목은 원목에서 발이시 대 직경이 클수록 접목 단수를 높일 수 있는데 녹각영지 경우 대직경은 4cm로 일반 영지보다 2배 높게 나타나 직경만으로는 녹각지가 대목으로 적당하지만 녹각영지의 경우 접목 활착시 활착율이 떨어졌고 활착기 간도 일반영지보다 2일정도 더 소요되었고 접목 친화율도 떨어져 대목용으로는 맞지 않고 3단 이후의 접수로 사용됨이 좋을 것으로 판단된다.In the case of suitable trees, the larger the diameter of the lumber can be, the higher the number of grafting stages can be.In the case of nocturnal land, the large diameter is 4cm, which is 2 times higher than that of general areas. It took about two days longer than normal estates, and the grafting affinity was also low, so it would not be suitable for large trees and it would be better to use it after the third stage.

대목 및 접수가 분화로 구성될 때 대의 길이에 따라 분화 단수, 분재의 균형, 트리수형에서 가지 수 등에 많은 영향을 준다.When the tree and the reception are composed of differentiation, the length of the stage affects the number of differentiation stages, the balance of bonsai, and the number of branches in the tree tree.

따라서 대목 및 접수의 대 길이는 3∼5cm일때 가장안정된 분재 수형을 구성 할 수 있었다.Therefore, when the large length of large and small trees was 3 ~ 5cm, the most stable bonsai tree could be composed.

표3. 자실체 생육 30월 경과시 대쪽의 생육상황Table 3. Growth situation of fruiting body after 30 month of fruiting body growth

품종  kind 원기형성기간(일) Rejuvenation period (days) 대길이 (cm) Length (cm) 대직경 (cm) Large diameter (cm) 원목당 발이수(개) Fees per tree () 접목활착율 (%) Graft Sliding Rate (%) 활착기간 (일) Life span (days) 일반영지  General estates 10 10 4 4 2 2 9 9 100 100 3 3 녹각영지  Nogga 20 20 7 7 4 4 3 3 60 60 5 5

2. 접목적정온도 규명2. Identification of graft suitable temperature

영지의 접목은 버섯류의 생장 생육시 상처가 발생하였을 때 적정 환경 조건에서 상처부위에 새로운 균사 생장이 이루어지게 되는데 이러한 원리를 이용하여 두 영지를 접촉 활착 시킴으로써 접목이 이루어지게 된다.Grafting of ganoderma lucidum occurs when the mycelium grows and grows mycelia under the appropriate environmental conditions when a wound occurs. Grafting is achieved by contacting two ganoderma using these principles.

영지의 균사생장 기능온도는 10∼38℃이며, 최적온도는 25∼32℃이다.The mycelial growth function temperature of ganoderma lucidum is 10-38 degreeC, and the optimal temperature is 25-32 degreeC.

접목 후 활착을 유도하기 위해 온도를 18℃,20℃,24℃,30℃별로 활착율을 표4와 같이 조사하였다.In order to induce the sticking after grafting, the sticking rate was investigated for each temperature of 18 ℃, 20 ℃, 24 ℃, 30 ℃ as shown in Table 4.

접목 후 24∼28℃ 사이의 온도에서 좋운 활착율을 보였고 이 온도에서 30시간 후 접목 부위에서 하얀색의 균사 생장을 관찰 할 수 있었다. 20℃ 이하나 30℃이상에서 접목 부위를 살펴보면 30시간 경과 후 약간 균사 생장이 관찰되었으나 72시간 후 관찰하였을 때 접목 부위의 새로운 균사가 노랗게 변색되다 갈색으로 변하여 접목이 되지 않았다 표에서 보듯이 영지접목의 적정온도 24∼28℃, 활착 기간도 24∼30℃에서 일반영지는 3일, 녹각영지는 5일로 가장 양호 하였다.After grafting, it showed good sticking rate at the temperature between 24 ~ 28 ℃, and white hyphae growth was observed at 30 hours after grafting. When the grafting site was observed below 20 ℃ or above 30 ℃, the mycelial growth was slightly observed after 30 hours, but when it was observed after 72 hours, the new hyphae of the grafting site turned yellow and turned brown so that grafting was not possible. At the optimum temperature of 24 ~ 28 ℃, and the sticking period of 24 ~ 30 ℃, the common manor was 3 days and the green manor manor was 5 days.

표4. 접목 온도별 활착율 및 활착소요일수Table 4. Sliding Rate and Sliding Time by Grafting Temperature

처리온도(℃)  Treatment temperature (℃) 품종 kind 활착율(%) Ride rate (%) 활착기간(일) Length of stay (days) 1818 일반영지General estates 5050 77 녹각영지Nogga 3030 1010 2020 일반영지General estates 8585 55 녹각영지Nogga 6060 77 24 24 일반영지General estates 100100 33 녹각영지Nogga 6060 55 3030 일반영지General estates 8585 33 녹각영지Nogga 5050 55

3. 접목 적정습도 및 환기3. Grafting proper humidity and ventilation

균사 생장에 알맞은 원목 수분 함량은 42∼45% 내외가 적당하며 원목의 조직 세포가 고사된 상태에서 영지의균사 생장이 양호하며 버섯이 형성되기 시작하면 원목에 균사 생장이 완료되므로 접목 활착을 양호하게 하기 위하여 접목 전과 후 관수 했을 때는 활착이 양호하였지만 무처리구에서는 활착율이 극히 저조했다.The moisture content of wood suitable for mycelial growth is about 42 ~ 45%. The mycelial growth is good in the state where the tissue cells of the wood are killed. In the case of irrigation before and after grafting, the tackiness was good, but the tackiness rate was very low in the untreated area.

또한 접목 부위 생육생장을 균사생장 조건으로 전환시키고자 검정비닐 멀칭 3일간 배양했을 때 활착율이 가장 양호하였으며 접수 편각 가장자리 생장점 부위도 양호한 결과를 보여주었다.In order to convert the grafted growth into mycelial growth conditions, the cultivation rate was the best when incubated with black vinyl mulching for 3 days, and the incidence of the declination edge growth was also good.

비닐 멀칭을 3일 이상하였을 때 과습으로 인한 접목 가장자리와 편각 가장자리 생장점 부위에 곰팡이가 기생하였다.After 3 days or more of vinyl mulching, mold was parasitic on the growth point of the grafted and declination edges due to overheating.

4. 접목 방법 별 접목4. Grafting by Grafting Method

접목 방법이 분화 수형 구성에 미치는 영향을 알아보기 위해 대목과 접수를 V자 점목과 평 접목을 사용한 결과 접목활착 상황은 두 방법 모두 양호하였다. V자 접목을 하였을 때 아주 견고하여 수평수직방향의 힘에도 안정화를 보였고, 접수의 대 길이가 5cm이상의 접수에서는 좋은 접목방법이나 대 길이가 3∼5cm일 때는 접목 작업효율이 평접보다 낮았다. 이는 접수의 대 길이가 5cm이하에서 접목고정 작업이 어려워 대 길이가 감소하지 않는 평접이 보다 효율적이었다.In order to examine the effect of grafting method on the differentiation tree composition, the results of grafting and V-branch and flat grafting were good. When V-grafted, it was very strong and stabilized in the horizontal and vertical force. When the length of the reception desk was more than 5cm, the grafting work efficiency was lower than the leveling. This was more efficient when the base length of the reception was less than 5cm and the graft was difficult to fix.

5.접수 시기 선별5.Selection of reception time

영지 배재는 개량단목재배 방법으로 원목에 종균을 접종하여 68일 영지 재배사에서 배양한 후 하우스내에 비닐을 깔고 그 위에 원목배지를 10cm 사이를 뛰어 놓고 원목배지 상단 부위 종균 주입구의 마개를 제거하고 상단부위 비닐도 제거한 후 원목배지 상단부위에 모래로 덮어 충분한 관수를 하여 버섯을 발생시켰다.Ganoderma lucidum is inoculated with the seedlings in the wood by the improved timber cultivation method, incubated in the Ganoderma grower on the 68th day, and then placed in a house with vinyl, the wood medium jumped between 10cm, and the stopper of the spawn inlet at the top of the wood medium was removed. After removing the vinyl, the top of the wood medium was covered with sand to give enough watering to generate mushrooms.

버섯 발생부터 편각이 형성되기 전까지의 접수(5)와 대목(6)을 사용하여 접목을 하였을 경우 접수와 대목이 하나로 합쳐져 한 덩어리가 된다.In the case of grafting using mushrooms (5) and large tree (6) from mushroom generation until the declination is formed, the large and small pieces are merged into one.

따라서 접목 시기는 버섯 발생 후 편각이 형성되거나 편각의 표면이 황백색에서 적갈색∼자갈색으로 변하는 시기의 영지를 접수 및 대목으로 선택하는 것이 가장 양호하였다.Therefore, the grafting time was the best to select the territories when the declination is formed after the mushroom generation or when the surface of the declination changes from yellowish white to reddish brown to purple-brown.

표. 접목 시기별 접수 생존율 비교table. Comparison of reception survival rate by grafting time

접목시기 (발이 후 일수) Grafting time (days after footing) 접목본수 (개) Number of Grafts (pcs) 접목후 생존 개체율(개)  Survival Population Rate after Grafting () 3일3 days 5일5 days 7일7 days 1212 2020 2020 1010 00 1515 2020 2020 1212 55 1818 2020 2020 1818 1818 2020 2020 2020 2020 2020 2525 2020 2020 2020 2020

표에서 보는 바와 같이 발이 후 18일 이상 생육 한 것을 대목 및 접수로 사용하는 것이 가장 양호하였다As shown in the table, it was best to use that which grew more than 18 days after the foot was used as the tree trunk and the reception desk.

6. 접목 후 활착 환경 조건6. Grafting environmental conditions after grafting

접목 후 접목 부위의 활착 조건 외관과 습도 조건을 알아보기 위해 습도 조건으로 원목배지에 검정 비닐로 처리한 구와 무처리구로 구분하여 일반 재배사와 블록 재배사에서 재배하였다. 표에서 나타난 결과를 보면 무처리구에서는 접목 1일 후 접목 부위의 하얀 균사가 보이지 않고 접수가 말라 수축현상이 보였으며, 비닐 멀칭 처리구에서는 접목1일 후 접목 부위가 하얀 균사가 발생으로 접목이 양호하였으며 편각 생장점 부위도 햐얗게 균사가 생장하고 있음을 보여 줬다.In order to examine the appearance and humidity conditions of the grafting site after grafting, the plant was divided into black vinyl treated and non-treated lumber as a humidity condition. The results shown in the table showed that the white mycelium on the grafting site was not seen after 1 day of grafting in the non-treated group, and the reception was dry. In the vinyl mulching treatment, the grafting was good due to the white mycelia growing after 1 day of grafting. The growth point also showed white mycelium growth.

광은 일반 영지버섯재배 광조건과 일치한 90% 차광망 재배사나 어두운 상태(신문 읽을 정도)일 때 가장 좋은 접목 상황을 나타냈다.The light showed the best grafting condition in the 90% shading net grower which matched the general Ganoderma lucidum light condition or in the dark state (reading newspaper).

표. 접목 후 환경조건에 따른 생존율 비교table. Comparison of survival rate according to environmental conditions after grafting

환기조건  Ventilation condition 재배조건  Cultivation conditions 접목본수 Grafting 접목 후 생존(개) Survival after grafting (dog) 1일1 day 2일2 days 3일3 days 무처리 No treatment 하우스재배사House Cultivator 2020 00 00 00 블럭재배사Block Cultivator 2020 2020 1515 55 비닐멀칭 Vinyl mulching 하우스 재배사House grower 2020 2020 2020 2020 블럭재배사Block Cultivator 2020 2020 2020 2020

7.분화 수형구성 및 상품화7.Differentiated Tree Formation and Commercialization

영지를 분화로 구성하였을 때 수형은 일반분재의 트리수형목부작, 석부작 등 다양하게 구성하였다.When the ganoderma was composed of differentiation, the tree was composed of various trees such as tree tree tree and stone tree.

수형구성시 접목 단수는 접목전에 구상하여야 하며 대목 선정도 잘 선택하여야 좋은 모양을 구성할 수 있다.The number of grafting stages should be envisioned before grafting and the selection of large trees should be well selected to form a good shape.

1회 접목할 때 단수 선택은 표에서 보는 바와 같이 전체적 분화 구성, 분화상품 생산시기 결정, 작업효율, 재배사 관리등에 아주 중요한 요인으로 작용하였으며, 1회 접목 가능단수는 3단 동시접목이 가장 양호하였다.As shown in the table, the singular selection at the time of grafting was very important factor in overall differentiation composition, decision of production time of differentiation products, work efficiency, planter management, etc. .

이렇게 하므로서 녹각영지의 관사용재배로 농가의 소득을 높일 수 있고 버섯산업의 이미지 재고로 시장을 확대할 수 있다In this way, the cultivation of nocturnal lands can increase farmers' income and expand the market by reconstructing the image of the mushroom industry.

도 1은 본 발명의 원목 배지 도면1 is a wood badge of the present invention

도 2는 본 발명의 접붙이기 예시도Figure 2 is an exemplary view of grafting of the present invention

도 3은 본 발명의 제품 예시도3 is a product illustration of the present invention

도 4는 본 발명의 제품 사진이다.4 is a product picture of the present invention.

* 도면의 주요부분에 대한 부호설명* Explanation of symbols on the main parts of the drawings

1 : 원목 2 : 폴리에틸렌필름1: solid wood 2: polyethylene film

2a : 개방부 3 : 통기성마개2a: opening 3: breathable plug

4 : 종균 5 : 접수 4: spawn 5: received

6 : 대목6: large tree

Claims (1)

버섯시험장에서 분양받은 영지버섯종균을 증식배양하는 종균준비단계와 Spawn preparation stage for propagating and culturing Ganoderma lucidum spawns 평균직경 15~20cm, 길이 20cm, 수분함량 42~45중량% 되는 원목을 두겹의 내열성 폴리에틸렌튜브에 싸서 살균하는 원목배지 준비단계와 Log media preparation step of sterilizing the average diameter of 15 ~ 20cm, length 20cm, water content 42 ~ 45% by weight in a double layer of heat resistant polyethylene tube 원목배지에 종균을 접종하여 배양하고 대목버섯의 갓의 직경이 5~10cm로 성장되면 갓의 직경 2~5cm, 대길이3~5cm되는 접수용 버섯을 선택하여 V자접목, 평접목중에서 선택한 접목수단으로 1~4단으로 접목하는 접붙이기 단계와 Inoculate the seedlings on the wood medium and grow the diameter of the large sized mushrooms to 5 ~ 10cm. Then, select the mushrooms of 2 ~ 5cm in diameter and 3 ~ 5cm in diameter. Grafting step of grafting in 1 ~ 4 steps by means 접붙이 작업이 끝난 버섯을 재배사 내에서 24~38℃에서 3~5일간 활착시켜 접목이 성공되면 육성실에 옮겨 일정크기로 재배하고 재배가 완성되면 화분, 목부작, 석부작 숯과 같은 대목에 부착하여 관상용 제품으로 가공하는 가공단계로 제조되는 접 붙이기 수단을 이용한 관상용 영지버섯 제조방법After grafting the mushrooms in the cultivator, the mushrooms stick to the plant for 3 ~ 5 days at 24 ~ 38 ℃, and when the grafting is successful, they are transferred to the nursing room and grown to a certain size.When the cultivation is completed, they are attached to large trees such as flower pots, woodwork, and stonework charcoal. Method for preparing ornamental ganoderma lucidum mushroom using a gluing means manufactured by a processing step of processing into a product
KR1020080100865A 2008-10-14 2008-10-14 Method of culturing ganoderma lucidum karst KR101006734B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020080100865A KR101006734B1 (en) 2008-10-14 2008-10-14 Method of culturing ganoderma lucidum karst

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020080100865A KR101006734B1 (en) 2008-10-14 2008-10-14 Method of culturing ganoderma lucidum karst

Publications (2)

Publication Number Publication Date
KR20100041605A true KR20100041605A (en) 2010-04-22
KR101006734B1 KR101006734B1 (en) 2011-01-10

Family

ID=42217307

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020080100865A KR101006734B1 (en) 2008-10-14 2008-10-14 Method of culturing ganoderma lucidum karst

Country Status (1)

Country Link
KR (1) KR101006734B1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103070015A (en) * 2013-01-31 2013-05-01 于春艳 Method for cultivating cut flower glossy ganoderma
CN103125274A (en) * 2013-03-15 2013-06-05 福建农林大学 Grafting method between different types of ganoderma lucidum and potted landscape process
CN103650829A (en) * 2013-08-14 2014-03-26 欧庭武 Cultivation method of multi-layer purple lucid ganoderma bonsai

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101165269B1 (en) 2009-12-22 2012-07-19 박정헌 Method for plant in a pot of forest cultivating using Ganoderma lucidum
KR101281144B1 (en) 2012-11-08 2013-07-02 가평군청 Culturing method of mushroom and culture medium bag thereby

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2650365B1 (en) * 1989-07-28 1991-11-22 Caillau Ets QUICK COUPLING
JPH0665197A (en) * 1992-06-19 1994-03-08 Dai Ichi Seiyaku Co Ltd Production of pyrrolidinylacetamide derivative
KR20060039475A (en) * 2004-11-03 2006-05-09 김정석 Method of growing artificiality for sarassis crispa wulf. ex fr
JP2007202545A (en) 2006-02-01 2007-08-16 Yoshihisa Imagawa Mushroom cultivating method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103070015A (en) * 2013-01-31 2013-05-01 于春艳 Method for cultivating cut flower glossy ganoderma
CN103070015B (en) * 2013-01-31 2014-04-16 于春艳 Method for cultivating cut flower glossy ganoderma
CN103125274A (en) * 2013-03-15 2013-06-05 福建农林大学 Grafting method between different types of ganoderma lucidum and potted landscape process
CN103650829A (en) * 2013-08-14 2014-03-26 欧庭武 Cultivation method of multi-layer purple lucid ganoderma bonsai

Also Published As

Publication number Publication date
KR101006734B1 (en) 2011-01-10

Similar Documents

Publication Publication Date Title
CN106479901B (en) A kind of Xianggu mushroom strain and its application in method for cultivating mushroom
CN101524035B (en) Artificial culture method of fuscous dictyostelium boletes
CN104145718B (en) A kind of bamboo grove simulated wild glossy ganoderma culture technique
CN107996288A (en) A kind of method that hayashishita imitates Wild ecological cultivation Phellinus
CN103563644B (en) The cultivation method of a kind of large red glossy ganoderma
CN106613324A (en) Ganoderma undergrowth bionics wild cultivation method
KR101035898B1 (en) Novel lentinula edodes (berk.) pegler gna01
CN102934586B (en) Low-carbon and high-yield poria culture method
WO2017219394A1 (en) Seed production method of cultivated species of edible fungus and cultivation method of cultivated species and edible fungus prepared thereby
CN103583225A (en) Method for cultivating good-quality high-yield pleurotus geesteranus by means of cassava stems
US20200281214A1 (en) Fusarium solani and use of same in prevention and treatment of southern blight of dendrobium officinale kimura et migo
CN103650829B (en) Cultivation method of multi-layer purple lucid ganoderma bonsai
Chen Cultivation of the medicinal mushroom Ganoderma lucidum (curt.: Fr.) P. karst.(reishi) in north America
KR101006734B1 (en) Method of culturing ganoderma lucidum karst
CN110150013A (en) A kind of method of chinquapin mycorrhizal seedling raising
KR20090010922A (en) Mushroom bed cultivation method of lyophyllum shimeji
CN108029448B (en) The wild section of wooden Phellinus cultural method is imitated under a kind of natural forests
KR20150001859U (en) Complete culture hardwood medium of Poria cocos cultivated after inoculating spawn to the pine hardwood
KR100483333B1 (en) Production method of the cauliflower mushroom using fermented sawdust
CN108887077A (en) A kind of wild red ganoderma cultural method of high-efficient breeding
CN106748231A (en) A kind of selenium enriched oyster mushroom culture medium and application method containing Moringa bits
KR100349579B1 (en) Method for cultivating a paecilomyces japonica
CN101138322A (en) Cremastra appendiculata tissue culturing rhizome fungus root seedling raising technique
CN108076962A (en) A kind of wizened bacterium artificial cultivation method
CN112106596B (en) Retention cultivation method of ganoderma lucidum

Legal Events

Date Code Title Description
A201 Request for examination
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20131231

Year of fee payment: 4

FPAY Annual fee payment

Payment date: 20150122

Year of fee payment: 5

FPAY Annual fee payment

Payment date: 20151223

Year of fee payment: 6

FPAY Annual fee payment

Payment date: 20170605

Year of fee payment: 7

LAPS Lapse due to unpaid annual fee