CN103125274A - Grafting method between different types of ganoderma lucidum and potted landscape process - Google Patents
Grafting method between different types of ganoderma lucidum and potted landscape process Download PDFInfo
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- CN103125274A CN103125274A CN2013100826905A CN201310082690A CN103125274A CN 103125274 A CN103125274 A CN 103125274A CN 2013100826905 A CN2013100826905 A CN 2013100826905A CN 201310082690 A CN201310082690 A CN 201310082690A CN 103125274 A CN103125274 A CN 103125274A
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Abstract
The invention relates to a grafting method between different types of ganoderma lucidum and a potted landscape process, and belongs to the field of fungus industries. Fungus balls of staghorn fungus are attached to a grafting connection point. After a scion is fixed in grafting, under a suitable environment condition, and after germination of the fungus balls, the connection point and the scion are rapidly and permanently fixed. Grafting points of the ganoderma lucidum are not restricted by growing points, and scions of the ganoderma lucidum are not restricted by the types or fungus strains of connection points. Manufacturing of various kinds of potted landscape is benefited, and strong advantages are showed through application of a ganoderma lucidum living body potted landscape process. In the growth stage of secondary ganoderma lucidum living bodies, different special kinds of the ganoderma lucidum are combined to grow integrally through the adoption of a manual grafting method, colorful and graceful kinds of the ganoderma lucidum potted landscape are manufactured, the ganoderma lucidum potted landscape is plain and fresh and attractive in scene, an implied meaning of auspicious stands out, cultural life of people is enriched, and important significance is provided for further improvement of economic benefits and social benefits of ganoderma lucidum production.
Description
Technical field
The invention belongs to bacterium industry field, be specifically related to a kind of different heteroplastic graft methods of glossy ganoderma and potted landscape technique.
Background technology
Glossy ganoderma is Basidiomycetes Polyporaceae Ganoderma fungi, and its kind has red sesame, purple sesame and Ganoderma lucidum etc., and glossy ganoderma is medicinal has strengthening the body resistance to consolidate the constitution, cure ten thousand diseases, its function is confirmed, the well-informed magical effect is the Chinese traditional valuable ingredient of traditional Chinese medicine that has thousands of years medicinal histories, possesses very high medical value.Glossy ganoderma is as potted landscape, and simple and unsophisticated pure and fresh, scene is attractive in appearance, outstanding lucky implied meaning, and exquisite lovely, the noble quality that also fully shows glossy ganoderma dish garden is gorgeous.Glossy ganoderma dish garden has become with its unique classical design and deep artistic connotation the first-selection of decorateeing high-grade room, office, hotel, hotel, is also the fashion good merchantable brand of present, collection.Produce glossy ganoderma dish garden and have high economic benefit and social benefit.
Summary of the invention
The object of the present invention is to provide a kind of different heteroplastic graft methods of glossy ganoderma and potted landscape technique.
For achieving the above object, the technical solution used in the present invention is as follows:
At first the present invention provides a kind of glossy ganoderma different heteroplastic graft methods, comprises the following steps:
(1) cultivating in bag Ganoderma lucidum obtains deer horn shape fruit body, and obtains the bacterium ball with the Ganoderma lucidum liquid culture;
(2) other kind glossy ganoderma of cultivating in bag obtains mature sporophore, and its cap is kidney shape, semicircle or subcircular, and this fruit body as scion, and is got off along the base portion truncation with sterile blade;
(3) the different heteroplastic graft methods of glossy ganoderma:
A, grafting environment: require surrounding air relative moisture to reach 85%~90%, 26~28 ℃ of temperature, illumination every day 12 hours, intensity of illumination 50 lx;
B, grafting: take deer horn shape sporophore growth point or away from the growing point place as the grafting point, on grafting point, first with sharp blade, epidermis is put down and cut 1 cutter, bacterium ball and scion are put on grafting point, and with bamboo let fixedly scion and grafting point, and the lid film is stuffy, forbid before not surviving after grafting to spray water, treat that the mycelia on scion and grafting point twists joint firmly, the scion growing point recovers growth, after glossy ganoderma survives namely by normal management.
Described other kind glossy ganoderma of step (2) is selected from red sesame, purple sesame.
The present invention has also protected a kind of glossy ganoderma dish garden technique: the glossy ganoderma of cultivating in bag different cultivars; the ganoderma lucidum fruitbody that obtains different shape, different colours and differ in size; and heteroplastic graft methods different from above-mentioned glossy ganoderma; the ganoderma lucidum fruitbody of selecting different shape, different colours and differing in size is as scion; grafting on different grafting points; the growth that is combined into one of the lucidum variety of different characteristics, produce glossy ganoderma dish garden.
The glossy ganoderma grafting method is generally to carry out between same kind or same bacterial strain, in the place's grafting scion of ganoderma lucidum fruitbody growing point, is being difficult for surviving away from growing point grafting scion.Adopt the bacterium ball of Ganoderma lucidum to be attached to grafting contact place, during grafting, fixedly after scion, under the adapt circumstance condition, the bacterium ball is sprouted afterwards contact and scion is permanently fixed fast.The grafting point of glossy ganoderma is not subjected to the restriction of growing point like this, and the scion of glossy ganoderma is not subjected to the kind of contact or the restriction of bacterial strain.Be conducive to produce the potted landscape of making a lot of variety, use glossy ganoderma live body potted landscape technique and have very strong advantage.
Strong according to the ganoderma lucidum fruitbody regeneration capacity, aging after, quality is hard, and the natural gloss of paint sample is arranged, prolonged characteristic of being stale-proof, what artificial cultivating in bag was the most characteristic is the lucidum varieties such as red sesame, purple sesame, deer horn sesame.Adopt artificial grafting method the growth that is combined into one of the lucidum variety of different characteristics at the ganoderma lucidum fruitbody vegetative stage, produce the colourful glossy ganoderma dish garden of polychrome, glossy ganoderma dish garden is simple and unsophisticated pure and fresh, scene is attractive in appearance, outstanding lucky implied meaning, enriched people's culture life, economic benefit and social benefit tool that further raising glossy ganoderma is produced are of great significance.
Embodiment
Embodiment one
1, the glossy ganoderma of conventional cultivating in bag different cultivars (red sesame, purple sesame, Ganoderma lucidum)
(1) batching pack.Culture medium prescription is: rice sweet oak wood chip 70%, wheat bran 20%, corn flour 7%, phosphate fertilizer 1%, gypsum 1% and brown sugar 1%.Claim sufficient raw material by formula, first the material such as wood chip, wheat bran, land plaster are mixed thoroughly when the preparation planting material, mix after sugar is water-soluble and mix thoroughly in material, the water content of material rests in 63%, pH value 5.8.Adopt the polypropylene plastics sacked material of 0.0045 centimetre of 17 cm x 33 cm x, limit rim compacting, degree of tightness is suitable, and every bag of weight in wet base is 700 grams approximately.Sack loud-speaker choma and sponge gag.
(2) bacterium bag sterilization, inoculation.To sterilize immediately after the composts or fertilisers of cultivating pack.121 ℃ kept 2 hours.Take out inoculation when material temperature is cooled to below 30 ℃ after sterilization.Inoculation is undertaken by the sterile working requirement, makes every effort to be swift in motion, accurately, and one bottle of (750ml bottle) wheat original seed can connect material bag 40 bags of left and right.Labelled after inoculation, indicate lucidum variety.
(3) the bacterium bag is cultivated.
Vaccinated bacterium bag is moved in culturing room, be emitted on culturing rack, send out the bacterium stage, culturing room keeps 28 ℃, relative air humidity 60%, and dark the cultivation checks and prevents and treats living contaminants every 5 days bacterium.When mycelium sends out full 3/4, move into the mushroom producing room fruiting.
(4) bacterium bag management of producing mushroom
Bacterium sack in mushroom producing room is removed the sponge gag, intensity of illumination 200~300lx in the control room, 25~26 ℃ of temperature, relative air humidity is brought up to 85-95%, controls suitable CO early stage
2Concentration promotes the stem elongation.When stem reaches certain length, add intense light irradiation, sufficient oxygen is provided, promote the cap elongation.Intensity of illumination 400~500lx in the control room, ventilation every day secondary.The stem apical growing point expands, and forms gradually cap, when cap grows the 2-3 circle, strengthen humidity, carries out water spray and ventilation operation.Red sesame cap is changed by white → pale yellow → Huang → bronzing, and purple sesame cap is changed by white → grey violet → puce.When cap edge white disappearance, the edge reddens or purpling, when the back side emits conidial powder, shows that fruit body is ripe, can gather.
2, conventional Ganoderma lucidum cell culture processes
(1) Ganoderma lucidum cell growth medium formula: potato 10%g, wheat bran 2.5%, corn flour 1.5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, dusty yeast 0.5%, brown sugar 1%, pH6.
(2) sterilization routinely, inoculation, shaking table shaken cultivation.
(3) obtain after filtration glossy ganoderma living cells (bacterium ball), be used for glossy ganoderma grafting point and connect use.
3, the different heteroplastic graft methods of glossy ganoderma
Under the environmental condition that suitable ganoderma lucidum fruitbody grows, adopt appropriate bacterium ball to put in the middle of scion and contact, fixedly scion and contact, get final product grafting survival about a week.
(1) grafting environment: require surrounding air relative moisture to reach 85%~90%, 26~28 ℃ of temperature.Illumination 50 lx.
(2) scion: according to the glossy ganoderma dish garden modeling requirement, select different shape, different colours and the ganoderma lucidum fruitbody that differs in size as scion, and scale off with sterile blade planar, put plastic sack interior stand-by.
(3) graft technology: on the grafting point away from the Antler Mythic Fungus sporophore growing point, first cut 1 cutter with sharp blade with epidermis is flat, bacterium ball and scion are put on contact, with bamboo let fixedly scion and contact, and it is stuffy to cover film.Forbid water spray before not surviving after grafting, by the management in a week, the mycelia on scion and contact will be twisted joint firmly, and scion growing point recovery is grown, and glossy ganoderma just can survive substantially, after surviving namely by normal management.
4, glossy ganoderma dish garden technique
According to the biological property of glossy ganoderma, cultivate the conditions such as the interior light of basket, temperature, water, gas by Artificial Control, reach fruit body and press down effect long, that encourage and change the direction of growth.Specific practice is:
(1) control the formation of stem and cap, just forming the bacterium bag of fruit body primordium.Be placed on temperature, humidity and illumination all in suitable large plastic crate, plastic crate external application plastic foil encases, acanthopore on the basket face, make a basket interior gas concentration lwevel reach 0.1%, at this moment the fruit body primordium in the basket only extends stem and does not form cap, after stem grows to certain-length, the bacterium bag is taken out in basket is placed on outside basket, because ventilation is good, the stem of elongation can form cap very soon.
(2) control the quantity of stem, the bacterium bag is selected the small-bore collar, can form single creature sesame of long stem, and the bacterium bag is selected the heavy caliber collar, grows the glossy ganoderma that grows thickly of many stems; Nutrient is sufficient, can grow the thick large-scale glossy ganoderma of a large handle.
(3) change the direction of stem and cap growth, according to glossy ganoderma, strong phototropism is arranged, in the sporophore growth stage, adopt the four sides shading, stem and cap are grown vertically upward.If adopt the one-sided light of seeing, can make stem and cap towards the direction of light growth is arranged.
(4) cultivation of double-deck cap forming cap and not dormant glossy ganoderma, then is put into plastic crate and is continued to cultivate, under the poor aeration environmental condition, the hyperplasia layer will occur below cap, cap is thickened, hyperplasia layer edge can grow the secondary stem and continue elongation and go down.When stem reach a certain height, take out the bacterium bag, recover good aeration condition, the secondary stem grows cap very soon, forms the glossy ganoderma of double-deck cap.
(5) fruit body that forms distortion can make in entity and be out of shape with the ultraviolet ray irradiation.With plastic paper wrapping, can make that the stem of growing is crooked, chap, or the cap that wilfully scabs occur.
(6) the fruit-body color depth is subjected to the impact of light power, and light hadron entity color is dark, otherwise shallow.
(7) glossy ganoderma dish garden
Can produce following potted landscape:
1. a pair of mandarin duck: take a pair of Ganoderma lucidum as mobile jib, be each of the red sesame of 3~5 centimetres, purple sesame at 8~10 cm height grafting bacteria cover diameters.
2. three states are friendly, cultivate three Ganoderma lucidums, and wherein two root graft bacteria cover diameters are each of 3~5 centimetres of red sesames, purple sesame.
3. a skill is solely elegant: cultivate many Ganoderma lucidums, control growth and promote with hormone and grow, make one long sturdy obvious.
4. fresh breeze promotion: three layers of red sesame or purple sesame graftings, one deck is than a floor height.
5. set each other off with one heart: cultivate two Ganoderma lucidums, the grafting diameter is each of the red sesame of 3~5 centimetres, purple sesame, then is integrated.
The above is only preferred embodiment of the present invention, and all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (3)
1. different heteroplastic graft methods of a glossy ganoderma is characterized in that: said method comprising the steps of:
(1) cultivating in bag Ganoderma lucidum obtains deer horn shape fruit body, and obtains the bacterium ball with the Ganoderma lucidum liquid culture;
(2) other kind glossy ganoderma of cultivating in bag obtains mature sporophore, and its cap is kidney shape, semicircle or subcircular, and this fruit body as scion, and is got off along the base portion truncation with sterile blade;
(3) the different heteroplastic graft methods of glossy ganoderma:
A, grafting environment: require surrounding air relative moisture to reach 85%~90%, 26~28 ℃ of temperature, illumination every day 12 hours, intensity of illumination 50 lx;
B, grafting: take deer horn shape sporophore growth point or away from the growing point place as the grafting point, on grafting point, first with sharp blade, epidermis is put down and cut 1 cutter, bacterium ball and scion are put on grafting point, and with bamboo let fixedly scion and grafting point, and the lid film is stuffy, forbid before not surviving after grafting to spray water, treat that the mycelia on scion and grafting point twists joint firmly, the scion growing point recovers growth, after glossy ganoderma survives namely by normal management.
2. different heteroplastic graft methods of glossy ganoderma according to claim 1, it is characterized in that: described other kind glossy ganoderma of step (2) is selected from red sesame, purple sesame.
3. glossy ganoderma dish garden technique, it is characterized in that: the glossy ganoderma of cultivating in bag different cultivars, the ganoderma lucidum fruitbody that obtains different shape, different colours and differ in size, and the different heteroplastic graft methods of glossy ganoderma according to claim 1, the ganoderma lucidum fruitbody of selecting different shape, different colours and differing in size is as scion, grafting on different grafting points the growth that is combined into one of the lucidum variety of different characteristics, is produced glossy ganoderma dish garden.
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Cited By (6)
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CN103688750A (en) * | 2013-12-16 | 2014-04-02 | 福建农林大学 | Manufacture method of living body lucid ganoderma bonsai |
CN104541938A (en) * | 2013-10-18 | 2015-04-29 | 杭州常青保健食品有限公司 | Merge-cultivating method of Ganoderma |
CN106818205A (en) * | 2016-12-19 | 2017-06-13 | 蚌埠鲲鹏食品饮料有限公司 | A kind of red sesame root process |
CN108293594A (en) * | 2017-10-20 | 2018-07-20 | 翔天农业开发集团股份有限公司 | A kind of moulding cultural method of ganoderma lucidum landscape type |
CN108587924A (en) * | 2018-04-20 | 2018-09-28 | 湖南农业大学 | A kind of Ganoderma Lucidum pompon product |
CN111512883A (en) * | 2019-02-02 | 2020-08-11 | 李志广 | Method for planting ornamental ganoderma lucidum antler and paint |
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Cited By (9)
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CN104541938A (en) * | 2013-10-18 | 2015-04-29 | 杭州常青保健食品有限公司 | Merge-cultivating method of Ganoderma |
CN104541938B (en) * | 2013-10-18 | 2018-06-08 | 杭州常青保健食品有限公司 | A kind of ganoderma lucidum merges cultural method |
CN103688750A (en) * | 2013-12-16 | 2014-04-02 | 福建农林大学 | Manufacture method of living body lucid ganoderma bonsai |
CN103688750B (en) * | 2013-12-16 | 2015-09-09 | 福建农林大学 | A kind of preparation method of live body glossy ganoderma dish garden |
CN106818205A (en) * | 2016-12-19 | 2017-06-13 | 蚌埠鲲鹏食品饮料有限公司 | A kind of red sesame root process |
CN108293594A (en) * | 2017-10-20 | 2018-07-20 | 翔天农业开发集团股份有限公司 | A kind of moulding cultural method of ganoderma lucidum landscape type |
CN108587924A (en) * | 2018-04-20 | 2018-09-28 | 湖南农业大学 | A kind of Ganoderma Lucidum pompon product |
CN108587924B (en) * | 2018-04-20 | 2021-02-12 | 湖南农业大学 | Ganoderma lucidum mycelium pellet product |
CN111512883A (en) * | 2019-02-02 | 2020-08-11 | 李志广 | Method for planting ornamental ganoderma lucidum antler and paint |
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