KR102590413B1 - Colostrum hydrolysis product and skin improvement composition containing it - Google Patents
Colostrum hydrolysis product and skin improvement composition containing it Download PDFInfo
- Publication number
- KR102590413B1 KR102590413B1 KR1020210167873A KR20210167873A KR102590413B1 KR 102590413 B1 KR102590413 B1 KR 102590413B1 KR 1020210167873 A KR1020210167873 A KR 1020210167873A KR 20210167873 A KR20210167873 A KR 20210167873A KR 102590413 B1 KR102590413 B1 KR 102590413B1
- Authority
- KR
- South Korea
- Prior art keywords
- colostrum
- skin
- present
- hydrolysis product
- composition
- Prior art date
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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Abstract
본 발명은 초유의 가수분해 산물과 이를 함유한 피부개선용 조성물에 관한 것으로써, 더욱 상세하게는 초유를 가수분해하여 생성된 가수분해 산물과 이를 유효성분으로 함유하는 피부개선용 조성물에 관한 것이다. 이러한 초유의 가수분해산물을 유효성분으로 함유하는 피부개선용 조성물은 피부장벽 강화, 면역증강 또는 주름개선에 탁월한 효과가 있으며, 화장료 조성물, 건강식품 또는 약제조성물 등으로 유용하다.The present invention relates to a hydrolysis product of colostrum and a skin improvement composition containing the same. More specifically, it relates to a hydrolysis product produced by hydrolyzing colostrum and a skin improvement composition containing the same as an active ingredient. The skin improvement composition containing the hydrolyzed product of colostrum as an active ingredient has excellent effects in strengthening the skin barrier, strengthening immunity, or improving wrinkles, and is useful as a cosmetic composition, health food, or pharmaceutical composition.
Description
본 발명은 초유의 가수분해 산물과 이를 함유한 피부개선용 조성물에 관한 것으로써, 더욱 상세하게는 초유를 가수분해하여 생성된 가수분해 산물과 이를 유효성분으로 함유하는 피부개선용 조성물에 관한 것이다.The present invention relates to a hydrolysis product of colostrum and a skin improvement composition containing the same. More specifically, it relates to a hydrolysis product produced by hydrolyzing colostrum and a skin improvement composition containing the same as an active ingredient.
인간이 가지고 있는 기관 중 가장 큰 기관이 피부이다. 피부는 표피와 진피로 구성이 되어있다. 표피는 지속적으로 세포가 분열을 하며, 여러 종류의 층을 만들게 된다. 이때 가장 바깥쪽의 세포들은 죽고 단단하게 되어 외부의 스트레스로부터 신체를 보호하여 주는 가장 뛰어난 방어기관 중의 하나이며, 세균 또한이 피부를 뚫지 못한다.The largest organ that humans have is the skin. The skin is composed of the epidermis and dermis. The epidermis cells continuously divide and create various types of layers. At this time, the outermost cells die and harden, making it one of the best defense organs that protects the body from external stress, and bacteria also cannot penetrate the skin.
피부의 경우 피지선과 땀샘을 가지고 있기 때문에 피지선에서는 피지를, 땀샘에서는 수분을 분비하여 인간 스스로 피지막을 만들어 내고 각질층을 보호하게 되는데, 이 때문에 사람의 피부는 항상 촉촉하며, 윤기가 흐르고 매끄러운 상태를 유지할 수 있게 된다.Since the skin has sebaceous glands and sweat glands, the sebaceous glands secrete sebum and the sweat glands secrete moisture to create a sebum film and protect the stratum corneum. For this reason, human skin is always moist, shiny, and smooth. It becomes possible.
사람의 경우 이 각질층의 수분함량이 20%가 되었을 때가 가장 이상적인 피부이며, 아름다움이 한껏 돋보이는 상태이다. 그러나 피부는 외부 환경에 영향을 받아 다양한 변화와 노화를 일으키게 되고, 특히 일광이나 다양한 외부 광선에 노출되어 피부 질환을 일으키게 된다.In the case of humans, the most ideal skin is when the moisture content of the stratum corneum reaches 20%, and this is a state where beauty stands out to the fullest. However, the skin is affected by the external environment, causing various changes and aging. In particular, exposure to sunlight or various external rays causes skin diseases.
특히, 피부가 자외선에 노출되는 경우 피부의 빛깔이 검어지게 되고, 사람마다 피부색이 검은 정도도 다양한 형태로 나타난다.In particular, when the skin is exposed to ultraviolet rays, the color of the skin darkens, and the degree of darkening of the skin varies from person to person.
이러한 피부색은 대부분 맑고 투명한 피부로 가꾸고 싶은 것이 대부분의 욕망이고, 특히 하얀 피부를 선호하는 경향이 뚜렷하다.For most people with this skin color, the desire is to have clear and transparent skin, and there is a clear tendency to prefer white skin in particular.
화장품이란 피부를 외부로부터 보호, 단장, 청결을 목적으로 하는 것이지만, 화장품의 조성물을 자세히 보게 되면 본래의 뜻과는 다르게 제품의 형성에 있어서 꼭 필요한 성분이 들어가게 되어 그 성분들이 오히려 피부를 자극할 수있다. 예를 들어, 유화제, 향료 그리고 방부제 등이 이에 속한다. 이들은 화학 반응 합성으로 만들어지는 경우가 대부분이기 때문에 민감성 피부의 경우, 염증반응, 알러지, 홍반 등을 동반할 확률이 증가하게 된다.Cosmetics are intended to protect, decorate, and clean the skin from the outside, but if you look closely at the composition of cosmetics, you will see that, contrary to the original meaning, ingredients necessary for the formation of the product are included, and those ingredients may actually irritate the skin. there is. For example, emulsifiers, flavorings, and preservatives are included. Since these are mostly made through chemical reaction synthesis, the probability of inflammatory reactions, allergies, and erythema increases in the case of sensitive skin.
최근 들어 천연 화장품에 대한 요구가 늘어나면서 각종 천연화장품이 개발되고 있는데 그 중에서도 우유나 초유를 이용하여 화장료를 제조하는 기술도 제안되어 있다.Recently, as the demand for natural cosmetics has increased, various natural cosmetics have been developed. Among them, a technology for manufacturing cosmetics using milk or colostrum has also been proposed.
특히, 기존의 초유에는 락토페린(Lactoferrin)이 존재하기 때문에 항염의 효과가 있다. 락토페린의 경우, Tumor Necrosis Factor-α(TNF-α) 이나 Interleukin-1(IL-1) 등의 조절에 대한 역할을 하는 것으로 익히 알려져 있다 (Yamanaka, 2003).In particular, existing colostrum has an anti-inflammatory effect because lactoferrin is present. Lactoferrin is well known to play a role in regulating Tumor Necrosis Factor-α (TNF-α) and Interleukin-1 (IL-1) (Yamanaka, 2003).
또한, 초유에는 Insulin-like Growth Factor-1(IGF-1)이 함유되어 있는데, IGF-1은 전체적인 성장을 촉진하며, 세포 성장과 증폭을 조절할 수 있는 능력을 가지고 있는 것으로 알려져 있다(Uruakpa et al, 2002).In addition, colostrum contains Insulin-like Growth Factor-1 (IGF-1), which is known to promote overall growth and has the ability to regulate cell growth and amplification (Uruakpa et al , 2002).
초유에 함유되어 있는 카세인(casein)의 경우 우유 전체 성분의 3%, 유단백질 중 약 80%를 차지하고 있고, 섭취시 우리 몸에 알러지를 일으키는 성분으로 밝혀져 있다(G. H. Docena et al, 1996). Casein, contained in colostrum, accounts for 3% of all milk components and approximately 80% of milk proteins, and has been found to be an ingredient that causes allergies in our bodies when ingested (G. H. Docena et al, 1996).
또한, 카세인은 화장품 성분으로 쓰이고 있으나, 연구가 충분히 진행되어 있지 않다.Additionally, casein is used as a cosmetic ingredient, but research has not been sufficiently conducted.
그 외에도, 초유에는 각질제거 성분이 함유되어 있으며(권유빈, et al. 2007), 멜라닌 색소를 억제하는 TGF-beta가 함유되어 있는 것으로도 알려져 있고(Martinez-Esparza M, et al, 1997), 또 초유에는 보습효과가 뛰어다나고 증명된 히알루론산이 많이 함유되어 있다(Pedersen, et al, 2014).In addition, colostrum contains exfoliating ingredients (Kwon Yu-bin, et al. 2007) and is also known to contain TGF-beta, which suppresses melanin pigment (Martinez-Esparza M, et al., 1997). Colostrum contains a lot of hyaluronic acid, which has been proven to have an excellent moisturizing effect (Pedersen, et al, 2014).
피부 미용이나 미백을 위해 사용하는 화장료 조성물로서는 전통적으로 피부에 발라 사용하는 화장품이 있으며, 식품으로 섭취하여 미용 효과를 나타내는 이너뷰티(iinner beauty; 먹는 화장료)도 개발되고 있다.Cosmetic compositions used for skin beauty or whitening include those that are traditionally applied to the skin, and inner beauty (edible cosmetics) that have beauty effects by ingesting food are also being developed.
특히, 천연 성분으로 각광을 받고 있는 우유나 초유의 경우 피부의 보습, 주름개선, 미백 등의 효과를 나타내는 화장료로 사용하는 것이 알려져 있다.In particular, milk and colostrum, which are in the spotlight as natural ingredients, are known to be used as cosmetics for skin moisturizing, wrinkle improvement, and whitening effects.
초유는 우유와는 달리 다양한 성분이 함유되어 있어 바람직한 성분으로 인식되어있으나, 초기 생산 시기에 수집된 초유는 쉽게 부패하여 보관이 어렵고 가공이 쉽지 않아서 그 활용이 크게 제한적이다.Unlike milk, colostrum is recognized as a desirable ingredient because it contains various ingredients. However, colostrum collected during the early production period is easily corrupted, difficult to store, and is not easy to process, so its use is greatly limited.
최근 본 발명자들에 의해 초유 발효산물에 대한 기능성 화장품이 연구된 바 있다. 이를 토대로 한국특허공개 제10-2019-0082159호 등에서는 초유 발효산물의 화장료 조성물이 피부 보습, 주름개선 및 미백 등에 효과가 있는 것으로 제안한 바 있다. Recently, functional cosmetics using colostrum fermentation products have been studied by the present inventors. Based on this, Korea Patent Publication No. 10-2019-0082159 and others suggest that cosmetic compositions made of colostrum fermentation products are effective in skin moisturizing, wrinkle improvement, and whitening.
또한, 기존에 이러한 초유를 이용한 화장품 조성물로써, 한국등록특허 제10-0619289호에서는 초유의 알부민을 미백제인 알부틴과 연계한 미백 화장품의 제조방법에 대해 공지되어 있고, 한국특허공개 제10-2016-0110829호에서는 우유 발효물의 피부미백용 효능이 제안되어 있으며, 한국특허공개 제10-2013-0108828호 에서는 우유 유래 올리고 펩타이드를 이용한 미백용 화장료 조성물이, 한국등록특허 제10-2186872호에서는 우유 엑소좀을 포함하는 피부미백용 조성물이 제안되어 있다.In addition, as a cosmetic composition using such colostrum, a method for manufacturing whitening cosmetics by linking the albumin of colostrum with arbutin, a whitening agent, is known in Korean Patent No. 10-0619289, and Korean Patent Publication No. 10-2016-0110829 In this paper, the efficacy of fermented milk for skin whitening is proposed, in Korean Patent Publication No. 10-2013-0108828, a cosmetic composition for whitening using milk-derived oligopeptides is proposed, and in Korean Patent No. 10-2186872, milk exosomes are proposed. A composition for skin whitening comprising:
또, 초유나 우유 이외에도 동물성이나 식물성의 다양한 천연자원을 가공하여 미백 등 여러 종류의 기능성을 가진 화장료 조성물이 다수 제안되어 있다.In addition, many cosmetic compositions with various functionalities, such as whitening, have been proposed by processing various natural resources of animal or vegetable origin in addition to colostrum and milk.
상기와 같이, 종래기술이 천연성분을 이용한 화장료 조성물 중에서, 특히 우유와 초유는 그 성분 구성이 인체에 매우 유용한 성분들로 구성되어 선호하는 경향이 있다.As described above, among cosmetic compositions using natural ingredients in the prior art, milk and colostrum in particular tend to be preferred because their composition consists of ingredients that are very useful to the human body.
유제품을 활용한 기술로서 상기 언급한 특허로 예시된 기술을 보면, 일부 초유나 우유를 그대로 이용하거나 발효 처리하여 활용하는 것이 알려져 있고, 초유 성분의 여러 효능과 알부틴에 의한 미백 효과 또는 초유나 우유의 발효나 추출물 등을 이용한 피부미백용 조성물 등이 제안되어 있다.Looking at the technology exemplified by the above-mentioned patent as a technology utilizing dairy products, it is known that some colostrum or milk is used as is or after fermentation, and the various effects of colostrum components and the whitening effect due to arbutin or the use of colostrum or milk Compositions for skin whitening using fermentation or extracts have been proposed.
그러나 이러한 종래기술들은 우유나 초유 발효 처리하는 등으로 가공하고 있으나, 아직도 이로 인한 피부미용 효과에 대한 효과가 충분하지 않고, 초유의 특성을 충분하게 활용하는 데는 부족함이 있다. However, these prior technologies are processed by fermenting milk or colostrum, but the skin beauty effect is still not sufficient, and there is a shortcoming in fully utilizing the properties of colostrum.
또한, 초유를 이용한 화장품 등으로 적용 가능한 실효성이 있는 화장료 성분에 관한 기술을 제대로 제안하고 있지 못하다.In addition, it is not possible to properly propose technologies for effective cosmetic ingredients that can be applied to cosmetics using colostrum.
본 발명은 상기와 같은 종래기술의 문제점을 해결하고, 초유 성분의 활용성을 연구하던 결과를 토대로 하여, 초유 성분을 가수분해하여 얻어지는 초유의 유제품 가수분해 산물의 제공과 이에 대한 새로운 용도의 제공을 해결과제로 한다.The present invention solves the problems of the prior art as described above and, based on the results of research on the utility of colostrum components, provides a dairy hydrolysis product of colostrum obtained by hydrolyzing colostrum components and provides a new use for the same. Make it a problem to solve.
따라서 본 발명의 목적은 초유를 가수분해 처리한 초유의 가수분해 산물을 제공하는데 있다.Therefore, the purpose of the present invention is to provide a hydrolysis product of colostrum obtained by hydrolyzing colostrum.
또한, 본 발명의 다른 목적은 초유의 가수분해 산물을 피부개선용 조성물로 적용하는 용도를 제공하는데 있다.In addition, another object of the present invention is to provide a use of the hydrolysis product of colostrum as a composition for improving skin.
상기와 같은 과제해결을 위하여, 본 발명은 초유를 가수분해한 초유의 가수분해 산물을 제공한다.In order to solve the above problems, the present invention provides a hydrolysis product of colostrum obtained by hydrolyzing colostrum.
본 발명의 바람직한 구현예에 의하면, 상기 초유의 가수분해는 단백질 가수분해 효소로 가수분해할 수 있다.According to a preferred embodiment of the present invention, the colostrum can be hydrolyzed using a proteolytic enzyme.
본 발명의 바람직한 구현예에 의하면, 상기 단백질 가수분해효소는 플라보자임, 프로타멕스, 파파인, 프로테아제, 뉴트라아제, 알칼라아제, 리파아제, 포스포타제, DNA글리코실라아제, 아밀라아제, 뉴클레아제, 헬리카아제로 이루어진 군 중에서 선택된 어느 하나 이상이 사용될 수 있다.According to a preferred embodiment of the present invention, the proteolytic enzyme is flavozyme, protamex, papain, protease, neutrase, alkalase, lipase, phosphotase, DNA glycosylase, amylase, and nuclease. , any one or more selected from the group consisting of helicases may be used.
또한, 본 발명은 상기한 초유의 가수분해 산물을 유효성분으로 함유하는 피부개선용 조성물로서의 용도를 제공한다.In addition, the present invention provides the use of the above-mentioned hydrolysis product of colostrum as a composition for improving skin containing the product as an active ingredient.
본 발명의 바람직한 구현예에 의하면, 상기 피부개선용 조성물은 피부장벽 강화, 면역증강 또는 주름개선용 조성물로 적용될 수 있다.According to a preferred embodiment of the present invention, the composition for improving skin can be applied as a composition for strengthening the skin barrier, enhancing immunity, or improving wrinkles.
본 발명의 바람직한 구현예에 의하면, 상기 피부개선용 조성물은 화장료 조성물, 먹는 화장품, 건강식품 조성물 또는 약제조성물로 적용될 수 있다.According to a preferred embodiment of the present invention, the composition for improving skin can be applied as a cosmetic composition, edible cosmetics, health food composition, or pharmaceutical composition.
본 발명에 따른 초유의 가수분해 산물은 가수분해를 통해 초유에 함유된 특정 유효성분을 가수분해함으로써 피부장벽강화, 면역증강, 주름개선효과가 우수하므로, 기존의 우유, 유청 또는 이들의 발효물 등 우유 원료의 가공물에 비해 현저하게 우수한 피부개선 효과를 발휘한다.The hydrolysis product of colostrum according to the present invention has excellent skin barrier strengthening, immune enhancement, and wrinkle improvement effects by hydrolyzing specific active ingredients contained in colostrum through hydrolysis, so it can be used in combination with existing milk, whey, or their fermented products. It has a significantly better skin-improving effect compared to processed products made from milk.
특히, 본 발명에 따른 초유의 가수분해 산물은 그 제조가 간단하고 용이하며, 종래부터 널리 이용되는 가수분해 처리에 의해 새로운 형태의 가수분해산물을 얻은 것이므로 인체 독성이 없고 안정성이 높다.In particular, the hydrolysis product of colostrum according to the present invention is simple and easy to manufacture, and since it is a new type of hydrolysis product obtained through a hydrolysis treatment that has been widely used in the past, it is non-toxic to the human body and has high stability.
이러한 본 발명에 따른 초유는 가수분해함으로써 그 초유의 가수분해 산물은 성분 구성에서도 초유에 비해 매우 우수한 성분 구성을 나타내게 된다.By hydrolyzing the colostrum according to the present invention, the hydrolysis product of the colostrum exhibits a very superior component composition compared to colostrum.
그러므로 본 발명에 따른 초유의 가수분해 산물을 이용하여 화장품 등을 제조하는 경우 기존에 알려진 우유의 유래 가공물에서 발현되는 효과와는 달리 초유를 가수분해 처리에 의해 생성된 가수분해 산물에 함유된 성분 구성으로 인해 피부개선 효과를 발휘하는 화장품 등의 제조에 매우 유용한 효과가 있다.Therefore, when cosmetics, etc. are manufactured using the hydrolysis product of colostrum according to the present invention, the composition of the components contained in the hydrolysis product produced by hydrolyzing colostrum is different from the effect expressed in the previously known processed product derived from milk. This has a very useful effect in the manufacture of cosmetics that have a skin-improving effect.
또한, 본 발명의 초유 가수분해 산물은 피부개선용 화장료 조성물 이외에도 피부개선에 효과가 있는 이너뷰티 조성물이나 건강식품 또는 약제조성물로도 활용이 가능하다.In addition, the colostrum hydrolysis product of the present invention can be used not only as a cosmetic composition for skin improvement, but also as an inner beauty composition, health food, or pharmaceutical composition that is effective in improving skin.
도 1은 본 발명의 실시예에서 제조한 초유의 가수분해 산물의 피부장벽강화 효과에 대하여 β-hexosaminidase 활성에 대한 실험결과를 대조군과 비교하여 나타낸 그래프이다.
도 2는 본 발명의 실시예에서 제조한 초유의 가수분해 산물의 면역증강 효과에 대하여 NO측정에 대한 실험결과를 대조군과 비교하여 나타낸 그래프이다.
도 3은 본 발명의 실시예에서 제조한 초유의 가수분해 산물의 주름개선 효과에 대하여 Elastase활성 억제에 대한 실험결과를 대조군과 비교하여 나타낸 그래프이다.
도 4는 본 발명의 실시예에서 제조한 초유의 가수분해 산물의 주름개선 효과에 대하여 Collagenase활성 억제에 대한 실험결과를 대조군과 비교하여 나타낸 그래프이다.Figure 1 is a graph showing the results of an experiment on β-hexosaminidase activity for the skin barrier strengthening effect of the hydrolysis product of colostrum prepared in an example of the present invention compared with the control group.
Figure 2 is a graph showing the results of an experiment on NO measurement for the immune-enhancing effect of the hydrolysis product of colostrum prepared in an example of the present invention compared with the control group.
Figure 3 is a graph showing the results of an experiment on the inhibition of Elastase activity for the wrinkle improvement effect of the hydrolysis product of colostrum prepared in an example of the present invention compared with the control group.
Figure 4 is a graph showing the results of an experiment on collagenase activity inhibition for the wrinkle improvement effect of the hydrolysis product of colostrum prepared in an example of the present invention compared with the control group.
이하, 본 발명을 하나의 구현예로써 더욱 상세하게 설명하면 다음과 같다.Hereinafter, the present invention will be described in more detail as an embodiment as follows.
본 발명의 초유의 가수분해 산물은 초유 원물보다 활용성이 우수한 가수분해 산물로써 그 자체가 새로운 물성을 가진다.The hydrolysis product of colostrum of the present invention is a hydrolysis product that is more useful than raw colostrum and has new physical properties itself.
본 발명에서 얻어진 초유의 가수분해 산물은 피부장벽 강화, 면역증강, 주름개선 효능이 우수하므로 기존에 화학합성이나 천연성분 유래의 다른 피부개선용 조성물 등을 대체 및 보완하기 위하여, 초유의 가수분해를 통하여 천연 화장품 조성물 등의 제조에 적용하면 특히 피부개선 효과를 증진시킨 새로운 용도의 피부개선용 조성물로 제공될 수 있다.The hydrolysis product of colostrum obtained in the present invention has excellent skin barrier strengthening, immunity strengthening, and wrinkle-improving effects. Therefore, in order to replace and supplement existing chemical synthesis or other skin improvement compositions derived from natural ingredients, hydrolysis of colostrum is used. When applied to the manufacture of natural cosmetic compositions, etc., it can be provided as a skin-improving composition for new purposes, especially with improved skin-improving effects.
본 발명은 가수분해 원료로써 초유를 효율적으로 사용하여 그 활용성을 높이고자 하는 것에서 출발한다.The present invention starts from the goal of efficiently using colostrum as a hydrolyzed raw material to increase its usability.
본 발명의 바람직한 구현예에 따르면, 초유를 가수분해시킨 가수분해 산물과 이에 대한 피부개선용 조성물을 제공한다.According to a preferred embodiment of the present invention, a hydrolysis product obtained by hydrolyzing colostrum and a skin improvement composition thereof are provided.
본 발명의 바람직한 구현예에 따르면, 우유나 유청 또는 이들의 발효물 등 다른 유제품의 가수분해 산물에 비해 초유의 가수분해 산물이 월등하게 우수한 피부개선 효과를 나타낸다.According to a preferred embodiment of the present invention, the hydrolysis product of colostrum exhibits a significantly superior skin improvement effect compared to the hydrolysis product of other dairy products such as milk, whey, or fermented products thereof.
본 발명에 의하면, 상기 초유의 가수분해 산물에 적용된 가수분해는 가수분해 효소에 의해 가수분해된 것이 바람직하다.According to the present invention, it is preferable that the hydrolysis applied to the hydrolysis product of colostrum is hydrolysis by a hydrolytic enzyme.
본 발명의 바람직한 구현예에 의하면, 초유에 대한 가수분해를 위해 적용될 수 있는 상기 가수분해 효소는 단백질 가수분해 효소가 바람직하게 사용될 수 있다.According to a preferred embodiment of the present invention, the hydrolytic enzyme that can be applied for hydrolysis of colostrum may preferably be a proteolytic enzyme.
본 발명의 바람직한 구현예에 의하면, 상기 단백질 가수분해효소로서는 플라보자임, 프로타멕스, 파파인, 프로테아제, 뉴트라아제, 알칼라아제, 리파아제, 포스포타제, DNA글리코실라아제, 아밀라아제, 뉴클레아제, 헬리카아제로 이루어진 군 중에서 선택된 어느 하나 이상이 사용될 수 있다. 더욱 바람직하게는 플라보자임, 프로타멕스, 파파인, 프로테아제, 뉴트라아제, 알칼라아제 중에서 하나 이상이 사용될 수 있다.According to a preferred embodiment of the present invention, the proteolytic enzymes include flabozyme, protamex, papain, protease, neutrase, alkalase, lipase, phosphotase, DNA glycosylase, amylase, and nuclease. , any one or more selected from the group consisting of helicases may be used. More preferably, one or more of flabozyme, Protamex, papain, protease, neutrase, and alkalase may be used.
본 발명의 바람직한 구현예에 따르면, 특히 가수분해의 전 또는 가수분해 이후에 카세인이 제거된 것, 살균 처리된 것 중에서 어느 하나 이상의 공정을 포함할 수 있다.According to a preferred embodiment of the present invention, any one or more processes may be included, in particular, casein is removed before or after hydrolysis, or sterilization is performed.
본 발명에서 사용되는 초유는 통상적으로 분만 후 3일 이내에 짠 젖을 바람직하게 사용할 수 있다.The colostrum used in the present invention is preferably milk expressed within 3 days after delivery.
본 발명에서 사용되는 초유는 소, 염소, 산양 등 가축동물의 초유가 사용될 수 있다.Colostrum used in the present invention may be colostrum from livestock animals such as cows, goats, and goats.
본 발명에서는 초유 중에서도 소 초유를 사용할 수 있으며, 여기서 초유는 통상적으로 염소, 산양 등 가축동물의 초유를 포함하는 개념이며, 이에 한정된 것은 아니다.In the present invention, bovine colostrum can be used among colostrums, and here, colostrum generally includes colostrum from livestock animals such as goats and goats, but is not limited thereto.
본 발명의 바람직한 구현예에 따르면, 초유를 이용하기 위해서는 예컨대 초유를 급속 냉각한 다음 저온 살균 처리하여 원료를 준비하는 단계를 거쳐 사용할 수 있다. 이 과정에서 지방이 제거된 초유를 원료로 사용할 수 있다.According to a preferred embodiment of the present invention, in order to use colostrum, for example, colostrum can be rapidly cooled and then pasteurized to prepare raw materials. Colostrum from which fat has been removed during this process can be used as a raw material.
본 발명에서의 저온 살균처리는 통상의 저온 살균법에 의해 수행할 수 있다.Low-temperature sterilization in the present invention can be performed by a normal low-temperature sterilization method.
본 발명의 바람직한 구현예에 따르면, 본 발명이 초유의 가수분해 산물은 제조 공정의 임의의 단계에서 카세인이 제거된 것일 수 있다.According to a preferred embodiment of the present invention, the hydrolysis product of colostrum according to the present invention may have casein removed at any stage of the manufacturing process.
본 발명의 바람직한 구현예에 따르면, 카세인 등의 제거를 위해 예컨대 응고효소 역할을 위한 효소 복합체를 사용할 수 있으며, 구체적으로는 예를 들어 렌넷(rennet)을 바람직하게 사용할 수 있다.According to a preferred embodiment of the present invention, for example, an enzyme complex for the role of a coagulation enzyme can be used to remove casein, etc. Specifically, for example, rennet can be preferably used.
한편, 본 발명의 바람직한 구현에에 따르면, 상기 초유는 지방이 제거된 것을 사용할 수 있다. 이러한 초유의 지방 제거는 통상적인 원심분리법, 여과방법, 지방 응집제 이용방법 등으로 수행될 수 있다.Meanwhile, according to a preferred embodiment of the present invention, the colostrum from which fat has been removed can be used. Removal of fat from colostrum can be performed by conventional centrifugation, filtration, or use of a fat coagulant.
본 발명의 바람직한 구현예에 따르면, 초유 가수분해 산물은 추가적으로 여과 단계, 살균 단계, 추출 단계 또는 동결건조 등의 하나 이상의 단계를 거쳐 제조될 수 있다.According to a preferred embodiment of the present invention, the colostrum hydrolysis product may be additionally prepared through one or more steps such as filtration, sterilization, extraction, or freeze-drying.
이와 같이, 본 발명에 따라 얻어진 가수분해 산물은 제조 후에 2회 또는 수회의 여과를 거쳐 혹시 있을지 모를 균을 제거하여 사용할 수도 있다.In this way, the hydrolysis product obtained according to the present invention can be used by filtering it twice or several times after production to remove any bacteria that may be present.
본 발명의 가수분해 공정은 예컨대 초유인 원료의 농도를 1∼50중량％로 하고, 가수분해 효소는 0.1∼5중량％로 첨가할 수 있다. 가수분해 공정 조건은 예컨대 40∼80℃에서 1∼12시간 가수분해할 수 있다. 이렇게 얻은 가수분해 산물을 여과하여 사용할 수 있다.In the hydrolysis process of the present invention, for example, the concentration of the raw material, which is colostrum, can be set to 1 to 50% by weight, and the hydrolytic enzyme can be added at 0.1 to 5% by weight. Hydrolysis process conditions can be, for example, hydrolysis at 40 to 80°C for 1 to 12 hours. The hydrolysis product obtained in this way can be filtered and used.
본 발명에 따라 상기와 같은 단계를 거쳐 얻은 가수분해 산물은 기존에 초유나 우유의 가공물로부터 유래된 어느 성분과도 상이한 매우 유용한 성질을 나타내는 것으로 확인되었다. 특히, 놀라울 정도로 우수한 피부개선 효과를 나타내는 것으로 확인되었다.It was confirmed that the hydrolysis product obtained through the above steps according to the present invention exhibits very useful properties that are different from any existing components derived from colostrum or processed milk. In particular, it was confirmed to have a surprisingly excellent skin improvement effect.
그러므로 본 발명은 초유의 가수분해 산물 자체로도 새로운 천연 가공물로서의 특징을 가진다.Therefore, in the present invention, the hydrolysis product of colostrum itself has the characteristics of a new natural processed product.
따라서 본 발명은 이러한 초유를 가수분해하여 제조된 초유의 가수분해 산물 자체를 포함한다.Therefore, the present invention includes the hydrolysis product of colostrum itself, which is prepared by hydrolyzing such colostrum.
본 발명의 바람직한 구현예에 의하면, 이러한 본 발명의 가수분해산물의 뛰어난 효과는 매우 적은 사용량에 의해서도 매우 우수한 효과를 나타낸다. 예컨대 초유를 이용하는 경우 0.1-500㎍/ml, 0.5-300㎍/ml, 1-200㎍/ml, 1-100㎍/ml, 더 적게는 1-50㎍/ml 에서도 우수한 효과를 나타낸다,According to a preferred embodiment of the present invention, the excellent effect of the hydrolyzed product of the present invention is achieved even with a very small amount of use. For example, when using colostrum, excellent effects are shown at 0.1-500㎍/ml, 0.5-300㎍/ml, 1-200㎍/ml, 1-100㎍/ml, and even at 1-50㎍/ml.
이러한 본 발명의 초유 가수분해 산물의 효과는 초유에 비해 더 월등한 조건의 효과를 보인다.The effect of the colostrum hydrolysis product of the present invention shows a superior effect under conditions compared to colostrum.
본 발명의 바람직한 구현예에 따르면, 상기와 같은 방법으로 제조된 가수분해 산물은 피부개선용 조성물에 여러 가지 성상으로 첨가할 수 있으며, 예컨대, 분말상, 액상, 나노캡슐, 복합체 등의 형태로 첨가될 수 있으며, 바람직한 예로써는 액상으로 첨가될 수 있다.According to a preferred embodiment of the present invention, the hydrolysis product prepared by the above method can be added to the skin improvement composition in various forms, for example, in the form of powder, liquid, nanocapsule, complex, etc. It can be added in liquid form as a preferred example.
본 발명에 따른 가수분해 산물을 통하여 β-hexosaminidase를 억제하여 피부장벽 강화 효과를 확인하였으며, 면역반응에 관여하는 Nitric Oxide (NO)를 확인하여 면역증강 효과를 확인하였고, 피부 주름개선에 관여하는 Collagenase와 Elastase의 활성 변화를 측정하여 주름개선 효과가 있는 것을 확인하였다.Through the hydrolysis product according to the present invention, the effect of strengthening the skin barrier was confirmed by inhibiting β-hexosaminidase, the immune enhancement effect was confirmed by confirming Nitric Oxide (NO), which is involved in the immune response, and Collagenase, which is involved in improving skin wrinkles. and Elastase activity were measured to confirm that it had a wrinkle-improving effect.
본 발명에 따르면, 가수분해 산물이 함유된 피부개선용 화장품 조성물로는 화장수(스킨로션), 영양로션, 영양크림, 마사지 크림, 영양에센스, 클렌져, 마스크팩 등의 화장료 제형으로 사용될 수 있으며, 특별히 여기에 한정하는 것은 아니며, 기타 다양한 기능성 효과를 위한 식품 조성물 또는 약제조성물로 사용될 수 있다.According to the present invention, the cosmetic composition for skin improvement containing hydrolysis products can be used in cosmetic formulations such as lotion (skin lotion), nutritional lotion, nutritional cream, massage cream, nutritional essence, cleanser, and mask pack, especially It is not limited to this, and can be used as a food composition or pharmaceutical composition for various other functional effects.
본 발명에 따른 가수분해 산물의 피부개선 화장료는 그 원료가 초유이고 이를 가수분해하는데 사용된 가수분해 효소는 종래부터 식품제조에 이용되고 있는 성분이므로, 그 가수분해 산물 자체에 대한 안전성이 높으며, 기존의 조성물에 비해 월등하게 우수한 피부개선 효과를 나타낸다.The skin improvement cosmetic of the hydrolyzed product according to the present invention is made of colostrum as its raw material, and the hydrolytic enzyme used to hydrolyze it is an ingredient that has been used in food production conventionally, so the hydrolyzed product itself is highly safe, and the hydrolyzed product itself is highly safe. It shows a significantly superior skin improvement effect compared to the composition of .
또한, 본 발명의 바람직한 구현예에 따르면, 상기 가수분해 산물은 피부개선용 효과를 발현하는 약제조성물로 적용 가능하다. 또한, 식품 조성물로서 건강식품, 이너뷰티용 조성물 등으로 활용이 가능하다.Additionally, according to a preferred embodiment of the present invention, the hydrolysis product can be applied as a pharmaceutical composition that exhibits skin-improving effects. In addition, it can be used as a food composition, health food, inner beauty composition, etc.
그러므로 본 발명의 바람직한 구현예에 따르면, 상기 가수분해 산물은 피부개선용 화장료 조성물로 매우 유용하고, 또한 피부개선용 의약품인 피부연고제, 경구제 등으로 사용할 수 있다.Therefore, according to a preferred embodiment of the present invention, the hydrolysis product is very useful as a cosmetic composition for skin improvement, and can also be used as a skin ointment, oral agent, etc., which are medicines for skin improvement.
본 발명의 바람직한 구현예에 따르면, 가수분해 산물을 약제조성물로 적용하는 경우, 예컨대 피부연고제로서 1-500㎍/ml로 피부에 수회 도포하여 적용할 수 있다.According to a preferred embodiment of the present invention, when the hydrolysis product is applied as a pharmaceutical composition, for example, it can be applied to the skin as a skin ointment at 1-500 μg/ml several times.
이러한 형태는 피부에 적용하는 피부개선 화장료를 적용한 화장품으로 제조하여 적용하는 경우에는 0.1-500㎍/ml로 구성하여 제조할 수 있다.This form can be manufactured at 0.1-500㎍/ml when manufactured and applied as a cosmetic product with skin improvement cosmetics applied to the skin.
본 발명의 바람직한 구현예에 따르면, 더욱 바람직하게는 어떤 경우라도 피부에 1-400㎍/ml로 적용할 수 있다.According to a preferred embodiment of the present invention, more preferably, it can be applied to the skin at 1-400 μg/ml in any case.
따라서 본 발명은 상기와 같은 가수분해 산물의 피부개선용 화장료 조성물을 포함한다.Therefore, the present invention includes a cosmetic composition for improving skin using the above hydrolysis product.
또한, 본 발명은 상기와 같은 가수분해 산물을 유효성분으로 함유하는 피부개선용 약제조성물을 포함한다. 그 예로서는 피부개선용 피부연고제를 포함한다.In addition, the present invention includes a pharmaceutical composition for skin improvement containing the above hydrolysis product as an active ingredient. Examples include skin ointments for skin improvement.
위와 같이, 본 발명에 따르면 상기와 같은 가수분해 산물을 피부개선용 조성물로 사용할 수 있으며, 이를 이용하여 화장품을 제조할 수 있을 뿐만 아니라 필요시 건강식품이나 피부연고제 등의 약제조성물로 적용할 수 있을 것이다.As above, according to the present invention, the above hydrolysis product can be used as a composition for skin improvement, and not only can it be used to manufacture cosmetics, but if necessary, it can be applied as a health food or pharmaceutical composition such as skin ointment. will be.
이하, 본 발명을 실시예에 의해 상세하게 설명하겠는바, 본 발명이 실시예에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail through examples, but the present invention is not limited by the examples.
실시예 : 초유 가수분해 산물의 제조Example: Preparation of colostrum hydrolysis product
초유 1리터를 준비한 다음 여기에 가수분해 효소를 2％(w/w) 첨가하고, 50℃ shaking incubator에서 2시간 처리하여 가수분해를 시행하였다. 가수분해를 거친 이후에는 85℃에서 2분간 가열 처리하여 효소 가수분해 반응을 종료하였다.After preparing 1 liter of colostrum, 2% (w/w) of hydrolytic enzyme was added thereto, and hydrolysis was performed by treatment in a 50°C shaking incubator for 2 hours. After hydrolysis, the enzyme hydrolysis reaction was terminated by heating at 85°C for 2 minutes.
가수분해 종료 후에 이를 3,000 RPM에서 30분간 원심분리하여 상층액을 취하여 0.22um 필터로 여과하였다. 가수분해 산물은 진공 동결 건조하여 사용한다.After completion of hydrolysis, it was centrifuged at 3,000 RPM for 30 minutes and the supernatant was collected and filtered through a 0.22um filter. The hydrolysis product is vacuum freeze-dried before use.
실험예 1 : 비만 세포를 이용한 피부장벽강화 효과 평가Experimental Example 1: Evaluation of skin barrier strengthening effect using mast cells
세포주는 RBL-2H3 비만 세포를 이용하여 실험을 수행하였다. 0.05 %의 트립신(trypsin)-EDTA (GIBCO BRL)로 플레이트에 부착되어 자란 RBL-2H3 세포를 띄워서 세포수 계산(Cellcounting)을 통하여 96 well-plate에 5 × 104cells/well에 분주하고 IgE를 20ng/ml의 농도로 처리한 뒤, 24시간 동안 배양하였다. 배양된 세포주를 Saraganian buffer로 교체한 다음, 교체된 buffer에 농도별로 희석한 시료(대조군 및 실시예인 시료 A)를 각각 1시간 전처리한 뒤, 250ng/ml의 Ag (DNP-BSA)를 함께 처리하였다. 양성대조군으로는 PP2를 처리하였다.The experiment was performed using RBL-2H3 mast cells as the cell line. RBL-2H3 cells grown attached to the plate were floated with 0.05% trypsin-EDTA (GIBCO BRL), and through cell counting, 5 × 10 4 cells/well were dispensed into a 96 well-plate and IgE was added. After treatment at a concentration of 20ng/ml, the cells were cultured for 24 hours. The cultured cell line was replaced with Saraganian buffer, and samples (control and example sample A) diluted by concentration in the replaced buffer were pretreated for 1 hour each, and then treated with 250ng/ml of Ag (DNP-BSA). . PP2 was treated as a positive control group.
시료를 처리한 후 15분 동안 이산화탄소 배양(CO2 incubation, Thermo)하였다. 세포의 배양액을 50ul 취해 96well plate에 옮긴 후, 동량의 1mM p-NAG (p-nitrophenyl-N-acethyl-β-D-glucosaminide)를 넣고 37℃에서 1시간 동안 반응시켰다.After processing the sample, it was incubated in carbon dioxide (CO2 incubation, Thermo) for 15 minutes. After taking 50ul of the cell culture and transferring it to a 96 well plate, an equal amount of 1mM p-NAG (p-nitrophenyl-N-acethyl-β-D-glucosaminide) was added and reacted at 37°C for 1 hour.
반응 후 150ul의 0.1M carbonate buffer (pH 10.5)를 넣어 반응을 종료시킨 뒤 405nm에서 흡광도를 측정하여 β-hexosaminidase의 효소활성을 측정하였다. 측정된 O.D 405를 가지고 백분율로 환산하였으며, Ag처리군에서 비만세포의 β-hexosaminidase 효소활성을 100% 기준으로 하여 처리된 시료에 의해 β-hexosaminidase의 효소활성이 억제된 정도를 비교하여 도 1에 나타내었다.After the reaction, 150ul of 0.1M carbonate buffer (pH 10.5) was added to terminate the reaction, and the enzyme activity of β-hexosaminidase was measured by measuring absorbance at 405nm. The measured O.D. 405 was converted into a percentage, and the degree to which the enzyme activity of β-hexosaminidase was inhibited by the treated sample was compared based on 100% of the β-hexosaminidase enzyme activity of mast cells in the Ag treatment group, as shown in Figure 1. indicated.
가수분해한 초유의 추출물이 초유의 원물보다 월등하게 우수한 억제 효과를 나타내었다.The extract of hydrolyzed colostrum showed a much better inhibitory effect than the raw colostrum.
실험예 2 : RAW 264.7 cell을 이용한 면역증강 효능 평가Experimental Example 2: Evaluation of immune enhancement efficacy using RAW 264.7 cells
RAW264.7 세포를 10% FBS가 첨가된 DMEM 배지를 이용하여 5×105 cells/well로 조절한 후 24 well plate 에 접종하고, 시료를 처리하고 24시간 배양하였다. 생성된 NO의 양은 NO detection kit (iNtRON)를 이용하여 측정한 값을 나타내었다. LPS 단독 처리군을 양성대조군으로 사용하였다. NO 발생량의 정도를 도 2에 나타내었다. 가수분해한 초유의 추출물이 초유의 원물보다 NO의 발생량을 더 크게 증가시킨 것을 확인하였다.RAW264.7 cells were adjusted to 5×10 5 cells/well using DMEM medium supplemented with 10% FBS and then inoculated into a 24 well plate, samples were processed, and cultured for 24 hours. The amount of NO produced was measured using a NO detection kit (iNtRON). The LPS-only treatment group was used as a positive control group. The degree of NO generation is shown in Figure 2. It was confirmed that the extract of hydrolyzed colostrum increased the amount of NO generated more than the raw colostrum.
실험예 3 : 주름개선 효능 평가Experimental Example 3: Wrinkle improvement efficacy evaluation
본 발명의 상기 각 실시예에 따라 수득된 가수분해 초유 추출물을 시료로 하여 피부주름의 억제 및 개선효과 검정시험으로서 elastase활성저해 시험(Elastase Inhibition Activity Test)을 실시하였다.An elastase inhibition activity test was conducted using the hydrolyzed colostrum extract obtained according to each of the above examples of the present invention as a sample to test the effect of suppressing and improving skin wrinkles.
엘라스틴(Elastin)을 분해하는 효소인 elastase의 활성을 측정하는 방법으로 elastase 기질인 N-Succinyl-Ala-Ala-Ala ρ-nitroaniline을 이용하여 ρ-nitroaniline이 분해되면서 생기는 색의 변화를 410 nm의 파장에서 흡광도를 측정함으로써 elastase 활성을 측정하는 방법이다. 완충액은 pH 8.0, 0.267 M Trizma-HCl (Sigma), 기질액은 8.8 mM N-Succinyl-(Ala)3 ρ-nitroaniline (Sigma), 효소액은 돼지췌장 elastase를 10 ㎍/ml (Sigma)의 농도로 사용하였다. 완충액 60 μl, 기질액 20 μl와 초유, 가수분해 초유 추출물 각각을 농도별로 정제수에 녹여 100 μl로 한 시료액을 섞은 후, 효소액 20 μl를 넣어 25℃ 항온수조에서 15분간 반응시켜 ρ-nitroaniline의 생성량을 마이크로 플레이트 판독기를 이용하여 파장 405 nm에서 측정한다.This is a method of measuring the activity of elastase, an enzyme that decomposes elastin, using the elastase substrate N-Succinyl-Ala-Ala-Ala ρ-nitroaniline, and measuring the color change that occurs as ρ-nitroaniline is decomposed at a wavelength of 410 nm. This is a method of measuring elastase activity by measuring absorbance. The buffer solution was pH 8.0, 0.267 M Trizma-HCl (Sigma), the substrate solution was 8.8 mM N-Succinyl-(Ala)3 ρ-nitroaniline (Sigma), and the enzyme solution was porcine pancreatic elastase at a concentration of 10 ㎍/ml (Sigma). used. 60 μl of buffer solution, 20 μl of substrate solution, colostrum, and hydrolyzed colostrum extract were each dissolved in purified water at different concentrations to make 100 μl of the sample solution, mixed, then 20 μl of enzyme solution was added and reacted in a constant temperature water bath at 25°C for 15 minutes to obtain ρ-nitroaniline. The amount produced is measured at a wavelength of 405 nm using a microplate reader.
한편, 상기 elastase 활성도를 측정하기 위한 대조군은 시료 대신 정제수를 넣어 같은 방법으로 측정하였으며, 효소액 대신 정제수를 넣어 각각에 대한 색 보정값을 얻은 경우를 설정하였다(참고문헌: Lim, Suk-Won외, The effect of two terpenoids, ursolic acid and oleanolic acid on epidermal permeability barrier and simultaneously on dermal functions, 대한화장품학회지, 29(2), 205-232, 2003).Meanwhile, the control group for measuring elastase activity was measured in the same way by adding purified water instead of the sample, and the color correction value for each was obtained by adding purified water instead of the enzyme solution (References: Lim, Suk-Won et al., The effect of two terpenoids, ursolic acid and oleanolic acid on epidermal permeability barrier and simultaneously on dermal functions, Journal of the Korean Cosmetic Society, 29(2), 205-232, 2003).
도 3을 보면 가수분해한 초유의 추출물이 초유 원물보다도 더 elastase 효소 활성을 억제시키는 것으로 나타났다.Looking at Figure 3, it was shown that the extract of hydrolyzed colostrum inhibited elastase enzyme activity more than the raw colostrum.
실험예 4 : 주름개선 효능 평가Experimental Example 4: Wrinkle improvement efficacy evaluation
본 발명의 상기 각 실시예에 따라 수득된 가수분해 초유 추출물을 시료로 하여 피부주름의 억제 및 개선효과 검정시험으로서 collagenase활성저해 시험(collagenase Inhibition Activity Test)을 실시하였다.A collagenase inhibition activity test was conducted using the hydrolyzed colostrum extract obtained according to each of the above examples of the present invention as a sample to test the effect of inhibiting and improving skin wrinkles.
콜라겐(Collagen)을 분해하는 효소인 collagenase의 활성을 측정하는 방법으로 collagenase 기질인 collagenase을 이용하여 형광표지된 collagen이 분해되면서 생기는 형광의 변화를 Ex490, Em520에서 측정함으로써 collagenase 활성을 측정하는 방법이다. 100X농도로 희석한 시료 1ul와 collagenase 효소 5ul, assay buffer 44ul를 섞어 총 50ul가 되게 만든다. 양성대조군으로는 (1, 10)-Phenanthroline을 사용한다.This is a method of measuring the activity of collagenase, an enzyme that decomposes collagen, by measuring the change in fluorescence that occurs as fluorescently labeled collagen is decomposed using collagenase, a collagenase substrate, at Ex490 and Em520. Mix 1ul of sample diluted to 100X concentration, 5ul of collagenase enzyme, and 44ul of assay buffer to make a total of 50ul. (1, 10)-Phenanthroline is used as a positive control.
그 뒤 collagenase substrate 2ul와 assay buffer 48ul를 섞은 reaction solution을 넣어 최종 부피가 100ul가 되게끔 한 뒤 37℃에서 30~60분간 kinetic mode로 Ex/Em=490/520값을 측정한다.After that, add a reaction solution mixed with 2ul of collagenase substrate and 48ul of assay buffer to make the final volume 100ul, and measure the value of Ex/Em=490/520 in kinetic mode at 37℃ for 30~60 minutes.
한편, 상기 collagenase 활성도를 측정하기 위한 대조군은 시료 대신 정제수를 넣어 같은 방법으로 측정하였으며, collagenase 효소 저해능은 아래와 같이 계산하였다.Meanwhile, the control group for measuring collagenase activity was measured in the same way by adding purified water instead of the sample, and the collagenase enzyme inhibition ability was calculated as follows.
% relative inhibition=[△RFU% relative inhibition=[△RFU (EC)(EC) -△RFU-△RFU (sample)(sample) ]/△RFU]/△RFU (EC)(EC) X100X100
도 4를 보면 가수분해 초유 추출물이 일반 초유 추출물보다도 collagenase 효소활성도를 더 크게 감소시키는 것을 알 수 있다.Looking at Figure 4, it can be seen that the hydrolyzed colostrum extract reduces collagenase enzyme activity to a greater extent than the regular colostrum extract.
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| KR100423708B1 (en) * | 2000-09-27 | 2004-03-24 | 주식회사 한국메디칼푸드 | Agent of immunological enhancement comprising colostral fractions as active ingredient, method of the preparation for the same and its use |
| KR100619289B1 (en) | 2004-01-29 | 2007-04-12 | 종근당건강 주식회사 | Beauty product manufacturing method for using very beginning milk and arbutin and thereof product |
| JP2012188384A (en) * | 2011-03-10 | 2012-10-04 | Snow Brand Milk Products Co Ltd | Skin-beautifying agent |
| KR20130108828A (en) | 2012-03-26 | 2013-10-07 | 서울대학교산학협력단 | Composition for skin lightening comprising oligopeptide |
| KR20160110829A (en) | 2015-03-13 | 2016-09-22 | 주식회사한국야쿠르트 | The milk fermented product using Lactobacillus paracasei HY7301 for skin whitening and products containing thereof as effective component |
| KR102311097B1 (en) | 2017-12-29 | 2021-10-08 | 주식회사 팜스킨 | Aerobic fermented product of colostrum |
| KR102379300B1 (en) * | 2018-12-14 | 2022-03-29 | 이호 | Peptide derived from a biomimetic foremilk, and method for producing the same, and manufacturing apparatus therefor |
| KR102186872B1 (en) | 2019-04-25 | 2020-12-04 | 경희대학교 산학협력단 | Skin whitening composition comprising milk exosomes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101974405B1 (en) * | 2018-11-30 | 2019-05-02 | 이호 | Cosmetic composition comprising hydrolyzate of colostrum whey protein for skin regeneration |
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