KR101885523B1 - Method for production of capsule with corn silk extract and milk, capsule therefrom - Google Patents
Method for production of capsule with corn silk extract and milk, capsule therefrom Download PDFInfo
- Publication number
- KR101885523B1 KR101885523B1 KR1020160167270A KR20160167270A KR101885523B1 KR 101885523 B1 KR101885523 B1 KR 101885523B1 KR 1020160167270 A KR1020160167270 A KR 1020160167270A KR 20160167270 A KR20160167270 A KR 20160167270A KR 101885523 B1 KR101885523 B1 KR 101885523B1
- Authority
- KR
- South Korea
- Prior art keywords
- milk
- capsule
- alginate
- steep liquor
- corn steep
- Prior art date
Links
- 235000013336 milk Nutrition 0.000 title claims abstract description 65
- 239000008267 milk Substances 0.000 title claims abstract description 65
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- 239000000284 extract Substances 0.000 title claims abstract description 60
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- 238000004519 manufacturing process Methods 0.000 title claims description 20
- 235000007244 Zea mays Nutrition 0.000 title 1
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- 235000010443 alginic acid Nutrition 0.000 claims abstract description 41
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- 229940072056 alginate Drugs 0.000 claims abstract description 39
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- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 8
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/133—Fruit or vegetables
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C2210/00—Physical treatment of dairy products
- A23C2210/40—Microencapsulation; Encapsulation of particles
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/502—Gums
- A23V2250/5026—Alginate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
본 발명은 옥수수수염물추출물 분말과 알긴산염을 우유에 넣고 혼합하여 혼합액을 제조하는 단계 (a); 및, 상기 혼합액을 다가 양이온을 포함하는 용액에 떨어뜨리는 단계 (b);를 포함하는 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 관한 것으로, 본 발명의 캡슐에 탑재된 옥수수수염물추출물을 통해 우유에 함유된 지방성분의 흡수에 따른 지방 축적을 방지할 수 있는데, 우유에 함유되어 있는 지방을 인위적으로 제거하지 않아도 저지방 우유를 마시는 것과 유사한 효과를 기대할 수 있는 것이다. 또한, 본 발명은 캡슐 형태의 새로운 우유 음료를 개발하여 제공함으로써, 우유의 소비증대에도 이바지할 수 있다. (A) preparing a mixture by mixing cornstarch water extract powder and alginate in milk; And (b) dropping the mixed solution into a solution containing a polyvalent cation. The present invention relates to a method for preparing a corn steep lemon extract and a milk-containing capsule, It is possible to prevent the accumulation of fat due to the absorption of the fat component contained in the milk. Even if the fat contained in the milk is not artificially removed, the effect similar to drinking the low fat milk can be expected. Further, the present invention can contribute to an increase in consumption of milk by developing and providing a new milk drink in the form of a capsule.
Description
본 발명은 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 관한 것으로, 더욱 상세하게는 옥수수수염물추출물 분말과 알긴산염을 우유에 넣고 혼합하여 혼합액을 제조하는 단계 (a); 및, 상기 혼합액을 다가 양이온을 포함하는 용액에 떨어뜨리는 단계 (b);를 포함하는 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 관한 것이다. The present invention relates to a method for preparing corn steep liquor and milk-containing capsules, and more particularly, to a method for producing corn steep liquor and milk-containing capsules, which comprises the steps of: (a) preparing a mixture by mixing cornstarch water extract powder and alginate in milk; And (b) dropping the mixed solution into a solution containing a polyvalent cation. The present invention also relates to a method for producing a corn steep liquor extract and a milk-containing capsule.
알지네이트(alginate)는 알진산 또는 알긴산으로도 불리는 다당류로서, 김, 우뭇가사리 등의 해조류의 표면에 풍부하게 포함되어 있다. 알지네이트는 만뉴로닉산(β-D-mannuronic acid)과 글루로닉산(α-L-gluronic acid)이 1,4 결합을 하여 형성된 화합물이다. 알지네이트는 아이스크림, 사탕 등의 식품에서 촉감 개선과 안정성 향상을 위해 많이 사용되고 있다. Alginate is a polysaccharide, also called alginic acid or alginic acid, which is abundantly contained in the surface of seaweeds such as mugwort and mugwort. Alginate is a compound formed by 1,4-bond of? -D-mannuronic acid and? -L-gluronic acid. Alginate is widely used for improving touch and stability in foods such as ice cream and candy.
알지네이트 비드 (alginate bead)는 신축성이 있는 알갱이로 식품용 캡슐의 제조를 위해 사용된다. 알지네이트 비드의 제법은 비교적 간단한 특징이 있다. 일반적인 제법은 우선 0.5~5%(w/v)의 알긴산나트륨을 증류수에 녹여 수용액을 만들고, 여기에 포집대상물질을 첨가하는 단계; 주사기나 분액 깔대기를 이용하여 수면으로부터 높이 10~30 cm에서 농도 0.1~2.0M의 다가 양이온 용액(CaCl2, SrCl2, BaCl2, AlCl3 등)에 방울방울 떨어뜨리는 단계;로 구성되는데, 순간적으로 알지네이트가 경화하여 비드로 형성된다. Alginate beads are stretchable granules and are used for the manufacture of food capsules. The preparation of alginate beads is relatively simple. A typical preparation method is to dissolve 0.5 to 5% (w / v) sodium alginate in distilled water to prepare an aqueous solution, and adding a substance to be collected to the solution; Dropping droplets of a polyvalent cation solution (CaCl 2 , SrCl 2 , BaCl 2 , AlCl 3, etc.) having a concentration of 0.1 to 2.0 M at a height of 10 to 30 cm from the water surface using a syringe or a separating funnel, The alginate is cured to form beads.
알지네이트 비드는 그 내부에 다양한 생리 기능성 물질을 탑재시켜 이를 장내로 전달하는 매개체의 역할을 수행할 수 있기 때문에, 식품에서 많이 활용되고 있는 실정이다.Alginate beads are widely used in foods because they can carry various physiological functional substances therein and serve as mediators for transferring them into the intestines.
본 발명에서는 내부에 옥수수수염물추출물과 우유를 탑재한 알지네이트 캡술을 개발하여 제공하고자 한다. In the present invention, it is intended to develop and provide alginate capsules containing corn beer water extract and milk therein.
본 발명은, 옥수수수염물추출물 분말과 알긴산염을 우유에 넣고 혼합하여 혼합액을 제조하는 단계 (a); 및, 상기 혼합액을 다가 양이온을 포함하는 용액에 떨어뜨리는 단계 (b);를 포함하는 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법을 제공한다. (A) preparing a mixture by mixing cornstarch water extract powder and alginate in milk; And (b) dropping the mixed solution into a solution containing a polyvalent cation. The present invention also provides a method for producing a corn steep liquor extract and a milk-containing capsule.
본 발명의 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 있어서, 상기 우유는, 바람직스럽게 농축유인 것이 좋다. In the method for producing the corn steep liquor extract and the milk-containing capsules of the present invention, the milk is preferably a concentrate.
본 발명의 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 있어서, 상기 알긴산염은, 일 예로 알긴산나트륨일 수 있다. In the method for producing the corn steep liquor extract and the milk-containing capsules according to the present invention, the alginate may be, for example, sodium alginate.
본 발명의 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 있어서, 상기 혼합액은, 바람직하게 글리세린, 인산이수소칼륨, 구연산 및 물을 더 포함할 수 있다. In the method for producing a corn steep liquor extract and a milk-containing capsule according to the present invention, the above-mentioned mixture preferably further comprises glycerin, potassium dihydrogenphosphate, citric acid and water.
본 발명의 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 있어서, 상기 혼합액은, 바람직하게 농축유 25ml를 기준으로, 알긴산나트륨 1.0~1.4g, 글리세린 0.6~1.0g, 인산이수소칼륨 0.05~0.15g, 구연산 0.01~0.1g, 옥수수수염물추출물 분말 1.0~1.4g, 물 60~80ml이 첨가되어 혼합되는 것이 좋다. In the method for preparing the corn steep liquor extract and the milk-containing capsules according to the present invention, the mixed liquid preferably contains 1.0 to 1.4 g of sodium alginate, 0.6 to 1.0 g of glycerin, 0.05 to 0.15 g of potassium dihydrogen phosphate g, citric acid 0.01 to 0.1 g, 1.0 to 1.4 g of cornstarch water extract powder, and 60 to 80 ml of water.
본 발명의 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 있어서, 상기 혼합액은, 바람직하게 중탕으로 가열하여 옥수수수염물추출물 분말과 알긴산염을 충분히 용해시키는 것이 좋다.In the method for producing the corn steep liquor extract and the milk-containing capsules according to the present invention, it is preferable that the mixed liquor is heated with a hot water to sufficiently dissolve the corn steep liquor extract powder and the alginate.
본 발명의 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 있어서, 상기 단계 (a)는, 바람직하게 혼합액 제조 후, 우유향을 첨가하는 것이 좋다. In the method for producing a corn steep liquor extract and a milk-containing capsule according to the present invention, the step (a) is preferably added with a milk flavor after the preparation of the mixture.
본 발명의 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 있어서, 상기 다가 양이온을 함유하는 용액은, 일 예로 젖산칼슘이 용해된 용액인 것이 좋다. 이때, 상기 젖산칼슘은, 바람직하게 용액 중 3~7%(w/v) 용해되어 있는 것이 좋다. In the method for producing the corn steep liquor extract and the milk-containing capsules according to the present invention, the solution containing the polyvalent cation is preferably a solution in which calcium lactate is dissolved. At this time, it is preferable that the calcium lactate is dissolved in 3 to 7% (w / v) of the solution.
본 발명의 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법에 있어서, 상기 혼합액은, 바람직하게 다가 양이온을 포함하는 용액에 한 방울씩 떨어뜨리는 것이 좋다. In the method for producing the corn steep liquor extract and the milk-containing capsules according to the present invention, it is preferable to drop the mixture in a dropwise manner, preferably in a solution containing a polyvalent cation.
본 발명은 옥수수수염물추출물 분말과 알긴산염을 우유에 넣고 혼합하여 혼합액을 제조하는 단계 (a); 및, 상기 혼합액을 다가 양이온을 포함하는 용액에 떨어뜨리는 단계 (b);를 포함하는 과정으로부터 제조된 옥수수수염물추출물 및 우유 함유 캡슐을 포함하는 음료를 제공한다. (A) preparing a mixture by mixing cornstarch water extract powder and alginate in milk; And (b) dropping the mixed solution into a solution containing a polyvalent cation. The present invention also provides a beverage comprising the corn steep liquor extract and the milk-containing capsules.
본 발명에서는 옥수수수염물추출물과 우유를 함유하는 알지네이트 캡슐을 제공한다. The present invention provides an alginate capsule containing corn steep liquor extract and milk.
본 발명의 알지네이트 캡슐에 탑재된 옥수수수염물추출물을 통해 우유에 함유된 지방성분의 흡수에 따른 지방 축적을 방지할 수 있다. 즉, 우유에 함유되어 있는 지방을 인위적으로 제거하지 않아도 저지방 우유를 마시는 것과 유사한 효과를 기대할 수 있는 것이다. The corn steep liquor extract of the present invention can prevent fat accumulation due to the absorption of the fat component contained in the milk. In other words, it is possible to expect a similar effect to drinking low-fat milk without artificially removing the fat contained in milk.
또한, 본 발명은 캡슐 형태의 새로운 우유 음료를 개발하여 제공함으로써, 우유의 소비 증대에도 이바지할 수 있다. Further, the present invention can contribute to an increase in consumption of milk by developing and providing a new milk drink in the form of a capsule.
도 1은 옥수수수염물추출물이 지방세포 분화유도에 미치는 영향을 보여준다. 'OSS'는 옥수수수염추출물을 의미한다.
도 2는 옥수수수염물추출물이 지방세포 내 지방축적에 미치는 영향을 보여준다. 'OSS'는 옥수수수염추출물을 의미한다.
도 3은 옥수수수염물추출물이 3T3-L1 세포에 미치는 세포독성을 보여준다.
도 4는 옥수수수염물추출물이 3T3-L1 세포에서 글리세롤 분비에 미치는 영향을 보여준다. 'OSS'는 옥수수수염물추출물을 의미한다.
도 5는 옥수수수염물추출물이 3T3-L1 세포에서 HSL, pSer563, pSer660 HSL 단백질의 발현에 미치는 영향을 보여준다. 'OSS'는 옥수수수염물추출물을 의미한다.Figure 1 shows the effect of corn hydrolyzate extract on induction of adipocyte differentiation. 'OSS' means corn beard extract.
Fig. 2 shows the effect of corn steep liquor extract on fat accumulation in adipocytes. 'OSS' means corn beard extract.
Figure 3 shows the cytotoxicity of corn hydrolyzate extract on 3T3-L1 cells.
Figure 4 shows the effect of corn hydrolyzate extract on glycerol secretion in 3T3-L1 cells. 'OSS' means corn beard water extract.
Figure 5 shows the effect of corn hydrolyzate extract on the expression of HSL, pSer563, and pSer660 HSL proteins in 3T3-L1 cells. 'OSS' means corn beard water extract.
본 발명은 옥수수수염물추출물 분말과 알긴산염을 우유에 넣고 혼합하여 혼합액을 제조하는 단계 (a); 및, 상기 혼합액을 다가 양이온을 포함하는 용액에 떨어뜨리는 단계 (b);를 포함하는 옥수수수염물추출물 및 우유 함유 캡슐의 제조방법을 제공한다. (A) preparing a mixture by mixing cornstarch water extract powder and alginate in milk; And (b) dropping the mixed solution into a solution containing a polyvalent cation. The present invention also provides a method for producing a corn steep liquor extract and a milk-containing capsule.
또한, 본 발명은 옥수수수염물추출물 분말과 알긴산염을 우유에 넣고 혼합하여 혼합액을 제조하는 단계 (a); 및, 상기 혼합액을 다가 양이온을 포함하는 용액에 떨어뜨리는 단계 (b);를 포함하는 과정으로부터 제조된 옥수수수염물추출물 및 우유 함유 캡슐을 포함하는 음료를 제공한다. Also, the present invention provides a method for preparing a mixture of a corn steep liquor and an alginate, And (b) dropping the mixed solution into a solution containing a polyvalent cation. The present invention also provides a beverage comprising the corn steep liquor extract and the milk-containing capsules.
본 발명에서는 우유의 섭취를 늘리는 방편의 일환으로, 새로운 형태의 우유 함유 음료 제형을 개발하여 제공하고자 한다. 본 발명에서는 우유를 알지네이트 캡슐에 탑재시켜 우유 함유 알지네이트 캡슐을 제조하는데, 이와 같이 제조된 우유 함유 알지네이트 캡슐은 음료에 첨가되어 사용될 수 있다. In the present invention, as part of a way to increase the uptake of milk, a new form of milk-containing beverage formulation is developed and provided. In the present invention, milk is loaded on alginate capsules to prepare milk-containing alginate capsules. The milk-containing alginate capsules thus prepared can be added to beverages and used.
알지네이트 캡슐이 음료에 첨가될 경우, 알지네이트 캡슐은 비중에 의해 바닥에 가라앉게 되는데, 소비자는 알지네이트 캡슐보다 큰 직경을 갖는 빨대를 사용하여 바닥에 가라앉아 있는 알지네이트 캡슐을 흡입하게 된다. 이때, 음료도 같이 빨아 올라오면서, 입안에는 음료와 알지네이트 캡슐이 동시에 존재하게 되고, 치아나 혀를 이용하여 알지네이트 캡슐을 터뜨리면, 우유가 밖으로 흘러 나와 음료와 같이 섞이면서, 독특한 풍미를 부여할 수 있다. When the alginate capsule is added to the beverage, the alginate capsule is submerged to the bottom due to specific gravity. The consumer sucks the alginate capsule sitting on the floor using a straw having a larger diameter than the alginate capsule. At this time, as the beverage is sucked up, the beverage and the alginate capsule are simultaneously present in the mouth, and when the alginate capsule is blown by using the tooth or the tongue, the milk flows out and can be mixed with the beverage to give a unique flavor.
한편, 우유는 지방을 많이 함유하고 있어, 지방에 대해 거부감이 있는 소비자들은 저지방 또는 무지방 우유를 찾고 있다. 하지만, 원유로부터 지방을 제거하는 과정에는 원유에 별도의 가공방법을 더해야 하는데, 비가공 원유를 선호하는 소비자들은 이와 같은 별도 가공방법에 대해 거부감을 갖고 있는 것이 현실이다.On the other hand, milk contains a lot of fat, and consumers who are reluctant to fat are looking for low - fat or non - fat milk. However, in the process of removing fat from crude oil, it is necessary to add a separate processing method to crude oil. Consumers who prefer non-processed oil have a feeling of refusal to such a separate processing method.
본 발명에서는 이와 같은 문제점을 인식하여, 본 발명 캡슐 내부에 지방축적저해 또는 지방분해능이 있는 옥수수수염물추출물을 첨가하여 상기와 같은 우유 내 지방에 대한 거부감을 해소한 것이다. The present invention recognizes such a problem and solves the above-mentioned rejection of the fat in the milk by adding the corn steep liquor extract having the fat accumulation inhibition or fat decomposition ability in the capsule of the present invention.
이하, 본 발명의 내용에 대해 각 단계별로 세분해서 상세히 설명하고자 한다. Hereinafter, the contents of the present invention will be described in detail by each step.
<단계 (a): <Step (a): 포접대상Enclosure target 물질 및 Materials and 알지네이트Alginate 함유 혼합액의 제조> Preparation of mixed liquid containing >
본 단계는 옥수수수염물추출물 분말과 알긴산염을 우유에 넣고 혼합하여 혼합액을 제조하는 단계이다. 본 단계를 통해 옥수수수염물추출물 분말과 알긴산염은 우유에 분산 또는 용해되게 된다. This step is a step of preparing a mixture by mixing corn aqua water extract powder and alginate in milk. Through this step, the corn aqua water extract powder and alginate are dispersed or dissolved in milk.
본 단계에서, 상기 우유는 바람직스럽게 농축유인 것이 좋다. 농축유를 사용하면, 유고형분 함량 증가로 인해 우유의 영향성분이 증가하는 효과가 있다.In this step, the milk is preferably a concentrate. The use of concentrated milk has the effect of increasing the influence components of milk due to the increase in milk solids content.
본 단계에서, 상기 알긴산염은 일 예로 알긴산나트륨일 수 있다. 알긴산(alginate)는 하기 단계 (b) 과정을 통해 캡슐을 형성하는 구조체로써의 역할을 수행하게 된다. In this step, the alginate may be, for example, sodium alginate. The alginate acts as a structure for forming a capsule through the following step (b).
본 단계에서는, 옥수수수염물추출물 분말을 첨가하는데, 지방축적저해 또는 지방분해능이 있어 우유 내 지방에 대한 거부감을 해소할 수 있다. In this step, corn stover water extract powder is added, and it has the ability to inhibit fat accumulation or to resolve fat, thereby relieving the feeling of resistance to the fat in milk.
본 단계에서, 상기 혼합액은, 바람직하게 글리세린, 인산이수소칼륨, 구연산 및 물을 더 포함할 수 있다. 글리세린은 캡슐 표면의 보호 역할을 하는데, 캡슐이 건조되어 변형되는 것을 방지한다. 인산이수소칼륨 및 구연산은 혼합액의 pH를 조절해 주는 역할을 수행한다. 물은 전체 '혼합물'을 균일하게 혼합하기 위한 일종의 용매 역할을 수행하도록 첨가하였다. In this step, the mixture liquid may preferably further contain glycerin, potassium dihydrogenphosphate, citric acid and water. Glycerin protects the surface of the capsule, preventing the capsule from drying and deforming. Potassium dihydrogenphosphate and citric acid serve to control the pH of the mixture. Water was added to perform a kind of solvent role to uniformly mix the entire 'mixture'.
본 단계에서, 상기 혼합액은, 바람직하게 농축유 25ml를 기준으로, 알긴산나트륨 1.0~1.4g, 글리세린 0.6~1.0g, 인산이수소칼륨 0.05~0.15g, 구연산 0.01~0.1g, 옥수수수염물추출물 분말 1.0~1.4g, 물 60~80ml이 첨가되어 혼합되는 것이 좋다. In this step, the mixed liquid preferably contains 1.0 to 1.4 g of sodium alginate, 0.6 to 1.0 g of glycerin, 0.05 to 0.15 g of potassium dihydrogenphosphate, 0.01 to 0.1 g of citric acid, 0.1 to 0.1 g of citric acid water extract powder 1.0 to 1.4 g and 60 to 80 ml of water are added and mixed.
본 단계에서, 상기 혼합액은 알긴산나트륨, 글리세린, 인산이수소칼륨, 구연산, 옥수수수염물추출물 분말을 농축유에 혼합한 후, 물을 첨가하는 것이 좋다. 이때, 충분히 용해시키기 위해 중탕으로 가열하는 것이 좋다. 중탕은 20~30분 정도 수행하는것이 바람직하다. In this step, it is preferable that the mixed solution is prepared by mixing sodium alginate, glycerin, potassium dihydrogenphosphate, citric acid, and corn steep liquor water extract powder in concentrated oil, followed by adding water. At this time, it is preferable to heat with a hot water bath to dissolve sufficiently. It is preferable that the hot water is performed for about 20 to 30 minutes.
한편, 본 단계에서, 상기 혼합액 제조 후, 추가로 우유향을 첨가하는 것이 좋다. 우유향이 첨가됨으로써, 캡슐 파쇄시 우유에 대한 관능을 바로 느낄 수 있다. On the other hand, in this step, it is preferable to add a milk flavor after the preparation of the mixed liquid. By adding milk fragrance, the sensation of milk can be immediately felt when the capsule is crushed.
<단계 (b): <Step (b): 포접대상물질이The inclusion target substance 포접된Enclosed 알지네이트Alginate 비드의Bead 제조> Manufacturing>
본 단계는 상기 단계 (a)에서 제조된 혼합액을 다가 양이온을 포함하는 용액에 떨어뜨리는 단계이다. 알지네이트를 함유하고 있는 용액을 다가 양이온을 포함하는 용액에 떨어뜨림으로써 알지네이트 비드를 제조할 수 있는데, 비드 내부에 우유 및 옥수수수염물추출물분말 등 혼합액에 함유되어 있는 물질들을 포접할 수 있다. This step is a step of dropping the mixed solution prepared in the step (a) into a solution containing polyvalent cations. Alginate beads can be prepared by dropping a solution containing alginate into a solution containing polyvalent cations. Inside the beads, substances contained in the mixture such as milk and corn hydrolyzate powder can be entrapped.
본 단계에서, 상기 다가 양이온을 함유하는 용액은, 2가 이상의 양이온을 함유하는 용액을 사용할 수 있는데, 일 예로 젖산칼슘이 용해된 용액일 수 있다. 젖산은 인체에 무해한 유기산으로써, 반응액 중에 존재하여도 무해하고, 소비자들의 거부감이 없기 때문이다. 이때, 상기 젖산칼슘은, 바람직하게 용액 중 3~7%(w/v) 용해되어 있는 것이 좋다. In this step, the solution containing the multivalent cations may be a solution containing two or more cations, for example, a solution in which calcium lactate is dissolved. Lactic acid is harmless to humans and is harmless even if present in the reaction solution, and there is no sense of rejection by consumers. At this time, it is preferable that the calcium lactate is dissolved in 3 to 7% (w / v) of the solution.
본 단계에서, 상기 혼합액은, 바람직하게 다가 양이온을 포함하는 용액에 한 방울씩 떨어뜨리는 것이 좋다. In this step, it is preferable that the mixed solution is dropped by one drop into the solution containing the polyvalent cation.
이하, 본 발명의 내용을 하기 실시예 및 실험예를 통해 더욱 상세히 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예 및 실험예에만 한정되는 것은 아니고, 그와 등가의 기술적 사상의 변형까지를 포함한다. Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, the scope of the present invention is not limited to the following embodiments and experimental examples, and includes modifications of equivalent technical ideas.
[[ 실험예Experimental Example 1: One: 옥수수수염물추출물의Corn beard water extract 지방축적억제 및 지방분해 효능 확인] Inhibiting fat accumulation and confirming lipid-lowering efficacy]
1) One) 옥수수수염물추출물Corn beard water extract 제조 Produce
옥수수수염을 건조 및 분쇄한 뒤 건조무게의 10.7배 만큼 증류수를 가해 60℃의 진탕 배양기 (shaking incubator)에서 24시간 동안 열수추출한 뒤 원심분리하였다. 그 후, 감압필터하여 불순물을 제거하고, 동결건조함으로써 분말 형태로 제조하였다. 이렇게 제조한 옥수수수염물추출물 분말을 하기 실험에서 사용하였다. After drying and crushing the corn beard, distilled water was added at a rate of 10.7 times the dry weight, and the mixture was subjected to hot-water extraction for 24 hours in a shaking incubator at 60 ° C, followed by centrifugation. Thereafter, the solution was subjected to pressure filtration to remove impurities and lyophilized to obtain a powdery form. The cornstarch water extract powder thus prepared was used in the following experiment.
2) 세포주 배양 및 지방적 측정2) Cell culture and lipid measurement
3T3-L1 세포는 10% FCS을 포함하는 DMEM에 100 U/mL 페니실린과 100 μg/mL 스트렙토마이신을 첨가하여 5% CO2, 37℃에서 배양하고 유지하였다. 3T3-L1 지방전구세포의 지방 세포로의 분화를 위해 6 웰 플레이트에 5×105cell/mL의 세포를 분주하여 세포가 충분히 밀집되게 배양하였다. 3T3-L1 cells were maintained in DMEM containing 10% FCS supplemented with 100 U / mL penicillin and 100 μg / mL streptomycin at 5% CO 2 at 37 ° C. For the differentiation of 3T3-L1 adipose precursor cells into adipocytes, cells were seeded at a density of 5 × 10 5 cells / mL in a 6-well plate and the cells were densely packed.
2일 후, 0.5 mM IBMX, 1 μM 덱사메타손(dexamethasone), 10 μg/mL 인슐린(insulin, MDI solution) 및 10% FBS를 포함하는 DMEM에서, 2일 동안 배양함으로써 분화를 개시하였다. After 2 days, differentiation was initiated by culturing for 2 days in DMEM containing 0.5 mM IBMX, 1 [mu] M dexamethasone, 10 [mu] g / mL insulin (MDI solution) and 10% FBS.
그 다음, 10 μg/mL 인슐린 및 10% FBS를 포함하는 DMEM으로 교환하여 2일 동안 분화를 진행하였다. 이후, 10% FBS만을 포함하는 DMEM에서 배양함으로써 세포 내 지방소립(lipid droplet)을 형성하는 지방세포로 분화시켰으며, 지방소립의 양은 Oil red O 염색하여 확인하였다. Then, the cells were exchanged with DMEM containing 10 [mu] g / mL insulin and 10% FBS for 2 days. Thereafter, the cells were cultured in DMEM containing 10% FBS alone to differentiate into adipocytes forming lipid droplets in the cells. The amount of lipids was determined by staining with Oil red O.
지방세포의 수를 확인하는 실험인 'hyperplasia(하이퍼플라시아)'는 분화를 개시하는 순간부터 실험이 종료되는 순간까지 배양 배지를 교환할 때마다 옥수수수염물추출물을 농도별로 처리하였으며, 지방세포의 크기를 확인하는 실험인 'hypertrophy(하이퍼트로피)'는 지방세포 분화유도가 완료되고 지방소립이 형성되기 시작되는 4일째부터(10% FBS만 포함하는 DMEM) 실험이 종료되는 순간까지 배양 배지를 교환할 때마다 옥수수수염물추출물을 농도별로 처리하였다.'Hyperplasia', an experiment to confirm the number of adipocytes, treated corn steep liquor water with concentration every time the culture medium was changed from the moment of initiation to the end of the experiment, The 'hypertrophy' experiment, which confirms the size, is performed from the 4th day after induction of adipocyte differentiation and lipid droplet formation begins (DMEM containing only 10% FBS) until the end of the experiment Each treatment of corn mustache water extract was treated by concentration.
실험결과는 도 1 및 도 2와 같이 나타났다. 도 1에서 보는 바와 같이, 옥수수수염물추출물 1 mg/mL의 처리에 의해 지방세포 분화가 억제됨을 확인할 수 있었고, 도 2에서 보는 바와 같이 옥수수수염물추출물 1 mg/mL의 처리에 의해 지방세포 내 지방축적이 억제됨을 확인할 수 있었다.The experimental results are shown in Figs. 1 and 2. As shown in FIG. 1, it was confirmed that the treatment of 1 mg / mL of corn hydrolyzate resulted in inhibition of adipocyte differentiation. As shown in FIG. 2, treatment with 1 mg / mL of corn steep liquor Fat accumulation was suppressed.
3) 세포생존율 측정3) Cell viability measurement
옥수수수염물추출물이 3T3-L1 지방세포에 독성을 나타내는지 확인하고자 MTT assay를 실시하였다. 세포 생존율은 MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide) 환원 방법을 이용하여 측정하였다. The MTT assay was performed to determine whether the extract of corn steep liquor was toxic to 3T3-L1 adipocytes. Cell viability was measured by MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide) reduction method.
10% FCS를 함유한 DMEM을 이용하여 3T3-L1 세포의 수를 1×104 cell/mL로 맞추고, 96 웰 플레이트에 100 μL씩 분주하였다. 이후, 5% CO2와 37℃ 조건에서 24시간 동안 배양하였다. 그 후, 배지를 제거하고, FCS를 함유하지 않은 DMEM에 옥수수수염물추출물을 농도별로 희석하여 100 μL씩 첨가한 후, 다시 24시간 동안 배양하였다. The number of 3T3-L1 cells was adjusted to 1 x 10 4 cells / mL using DMEM containing 10% FCS, and 100 μL aliquots were added to 96-well plates. Then, the cells were cultured at 5% CO 2 and 37 ° C for 24 hours. Thereafter, the medium was removed, 100 μL of corn steep liquor extract was added to the DMEM without FCS, and the cells were further cultured for 24 hours.
24시간 후, 옥수수수염물추출물을 함유하고 있는 배지를 제거하고, MTT를 p.H 7.4의 PBS(phosphate-buffered saline)에 5 mg/mL로 용해시키고, 0.45 μm 시린지 필터 (syringe filter)하여 여과하였다. 여과된 용액을 DMEM을 이용하여 10배 희석하고, 100 μL씩 각 웰에 분주하고 37℃에서 4시간 더 배양한 후 배지를 제거하였다. After 24 hours, the medium containing corn steep liquor was removed and MTT was dissolved in PBS (phosphate-buffered saline) at 5 mg / mL and filtered through a 0.45 μm syringe filter. The filtered solution was diluted 10-fold with DMEM, dispensed into each well at 100 μL, incubated at 37 ° C for additional 4 hours, and then the medium was removed.
플레이트의 완전한 건조를 위해 배지 제거 후, 30분간 실온에서 방치하였다. DMSO를 이용하여 플레이트에 남아있는 적자색의 포르마잔(formazan)을 용해하여, 570 ㎚에서 흡광도를 측정하였다. 이를 이용한 세포의 생존율은 다음과 수학식 1에 의해 계산하였다. After complete removal of the medium for complete drying of the plate, it was left at room temperature for 30 minutes. The red magenta formazan remaining on the plate was dissolved in DMSO and the absorbance was measured at 570 nm. The survival rate of cells using this was calculated by the following equation (1).
[수학식 1][Equation 1]
세포 생존율 (%) = [시료처리군의 흡광도/대조군의 흡광도] ×100 Cell survival rate (%) = [absorbance of sample treatment group / absorbance of control group] 占 100
실험결과는 도 3과 같이 나타났다. 도 3에서 보는 바와 같이, 본 발명의 옥수수수염물추출물은 세포독성이 없는 것으로 확인되었다. The experimental results are shown in Fig. As shown in FIG. 3, the corn steep liquor extract of the present invention was found not to be cytotoxic.
4) 글리세롤 분비량 측정4) Glycerol secretion measurement
분화된 3T3-L1 지방세포(adipocyte)에 0.1, 0.5, 1 mg/mL 농도의 옥수수수염물추출물을 24시간 동안 처리한 후, PBS를 이용하여 배양배지를 세척하고 세포를 이용하여 실험에 사용하였다. The cultured medium was washed with PBS, and the cells were used for the experiment. The cells were treated with the corn steep liquor extracts at different concentrations of 0.1, 0.5, and 1 mg / mL for 24 hours in the differentiated 3T3-L1 adipocytes .
세포는 어세이 버퍼 (assay buffer) 500 μL를 넣고, 용해시킨 뒤 사용하였다. 96-웰 플레이트에 세포 용해물 (cell lysate) 50 μL와 어세이 버퍼 46 μL, 글리세롤 프로보 (glycerol probe) 2 μL, 글리세롤 엔자임 믹스 (glycerol enzyme mix) 2 μL를 넣고, 37℃에서 30분간 반응시킨 후, 마이크로플레이트 리더 (microplate reader)를 이용하여 570 nm에서 흡광도를 측정하였다.Cells were lysed by adding 500 μL of assay buffer. To the 96-well plate, add 50 μL of cell lysate, 46 μL of assay buffer, 2 μL of glycerol probe and 2 μL of glycerol enzyme mix, and incubate at 37 ° C. for 30 minutes. Then, the absorbance was measured at 570 nm using a microplate reader.
실험결과는 도 4와 같이 나타났다. 도 4에서 보듯이 옥수수수염물추출물의 처리 농도가 증가할수록 글리세롤 함량이 증가한 것을 확인할 수 있었다. 이를 통해 옥수수수염물추출물의 처리에 의해 지방이 분해됨을 확인할 수 있었다. The experimental results are shown in Fig. As shown in FIG. 4, it was confirmed that the glycerol content was increased as the treatment concentration of corn steep liquor extract was increased. It was confirmed that the fat was decomposed by the treatment of corn steep liquor extract.
5) 세포 내 지질대사조절 효소의 단백질 발현 측정5) Measurement of protein expression of intracellular lipid metabolism-regulating enzyme
가) 세포 내 단백질 추출 및 정량A) Protein extraction and quantification in cells
배양이 완료된 3T3-L1 세포에 옥수수수염물추출물을 농도별로 24시간 처리한 뒤 배지를 제거하고, 세포 스크래퍼 (cell scraper)로 웰 바닥에 붙어있는 세포를 모두 회수하였다. 이후, 1.5mL 튜브로 옮기고, 프로테인 익스트랙션 키트 (protein extraction kit)를 사용하여 단백질을 추출한 뒤 80℃에서 보관하였다. The cultured 3T3-L1 cells were treated with corn steep liquor extracts for 24 hours at a concentration, and then the medium was removed, and cells adhering to the bottom of the wells were all recovered with a cell scraper. Then, the sample was transferred to a 1.5-mL tube, and proteins were extracted using a Protein extraction kit and stored at 80 ° C.
추출한 단백질 5 μL에 증류수 795 μL를 넣어 희석한 후 브래드포드(bradford) 시약 200 μL를 넣어준 후, 5초간 볼텍싱하여 섞어 준 후, 5분간 배양시키고 595nm에서 흡광도를 측정하였다. 표준물질로 BSA 용액을 이용하여 정량하였다.5 μl of the extracted protein was diluted with 795 μL of distilled water, 200 μL of bradford reagent was added, followed by vortexing for 5 seconds, followed by incubation for 5 minutes and absorbance at 595 nm. BSA solution was used as a reference material.
나) 전기영동 및 이동B) Electrophoresis and migration
정량이 완료된 단백질을 SDS와 베타-메르캅토-에탄올(β-mercapto-ethanol)을 포함한 샘플 버퍼에 3:1로 혼합한 후, 100℃에서 3분간 가열하여 단백질 샘플을 준비하였다. 단백질 샘플은 SDS-gel을 이용하여 전기영동하여 단백질 크기별로 구분한 뒤, 단백질을 PVDF 멤브레인으로 이동시켰다.After the quantified protein was mixed in a sample buffer containing SDS and beta-mercapto-ethanol (3: 1), the protein sample was prepared by heating at 100 ° C for 3 minutes. Protein samples were electrophoresed using SDS-gel and classified by protein size, and proteins were transferred to PVDF membrane.
다) All) 블록킹Blocking (Blocking) 및 항체 반응 (antibody reaction)(Blocking) and antibody reaction (antibody reaction)
단백질 이외의 부분에 항체가 반응하지 않도록 5% 탈지분유 용액 (skim milk solution)에 멤브레인을 2시간 동안 처리한 후, 멤브레인을 TBS-T 버퍼를 이용하여 세척하였다. 그 후, 표적 단백질에 반응하는 1차 항체 (1% BSA solution)를 2시간 동안 처리하고, 2차 항체 (5% skim milk solution)에 40분 동안 처리한 후 TBS-T 버퍼를 이용하여 세척하였다. 1차 및 2차 항체는 'Acris, Santa cruz, Cell signaling' 제품을 사용하였다.The membrane was treated with 5% skim milk solution for 2 hours to prevent the antibody from reacting with the other part of the protein, and the membrane was washed with TBS-T buffer. Then, the primary antibody (1% BSA solution) reacted with the target protein was treated for 2 hours, treated with the secondary antibody (5% skim milk solution) for 40 minutes, and washed with TBS-T buffer . The primary and secondary antibodies were 'Acris, Santa cruz, Cell signaling' products.
라) 화학발광 검출 (D) Chemiluminescence detection ( ChemiluminescenceChemiluminescence detection) detection)
멤브레인 상에서 항체 결합 유무를 확인하기 위하여 멤브레인에 웨스턴 검출 용액 (western detection solution, substrate, enhancer 각 1mL)을 도포한 후 암실에서 멤브레인을 감광시켜 밴드를 확인하였다. 단백질 발현 정도를 확인한 뒤 그 밴드의 강도를 Image J (National institutes of health, USA) 소프트웨어에 의해 분석 정량하였다. 이때, 내부 표준물로써 GADPH를 사용였다.In order to confirm the presence or absence of antibody binding on the membrane, a western detection solution (western detection solution, substrate,
마) 실험결과 E) Results of the experiment
실험결과는 도 5와 같이 나타났다. 도 5에서 보는 바와 같이, 옥수수수염 물추출물의 처리에 의해, 3T3-L1 세포에서 HSL, pSer563, pSer660 HSL의 발현량이 증가함을 확인할 수 있었다. 특히, 0.1 mg/mL 처리시 가장 높게 나타났다. The experimental results are shown in Fig. As shown in FIG. 5, the expression of HSL, pSer563, and pSer660 HSL in 3T3-L1 cells was increased by treatment with corn steep liquor extract. Especially, it was highest at 0.1 mg / mL treatment.
[[ 실시예Example 1: 본 발명 1: invention 농축유Concentrated oil 함유 캡슐의 제조] ≪ / RTI >
본 실시예에서는 본 발명의 농축유 함유 캡슐을 제조하였다. In this Example, the concentrated oil-containing capsules of the present invention were prepared.
하기 표 1에 기재된 성분들을 그 양만큼 농축유 25ml에 넣고 혼합하였다. 이때, 상기 혼합액에 물 71.35ml를 추가로 넣고, 중탕으로 30분 정도 가열하여 상기 성분들을 충분히 용해(또는, 분산)시켰다. 그 후, 우유향 0.3ml을 추가로 넣고 혼합하였다. Ingredients listed in Table 1 were added to 25 ml of concentrated milk by the amount and mixed. At this time, 71.35 ml of water was further added to the mixed solution, and the mixture was sufficiently heated (or dispersed) by heating in a hot water bath for 30 minutes. Thereafter, 0.3 ml of milk flavor was further added and mixed.
상기와 같이 제조한 혼합액을 젖산칼슘 5%(w/v)를 함유한 용액에 한 방울씩 떨어뜨려 비드를 제조하였다. 제조된 비드는 직경이 약 0.6~0.8 cm 정도였다. The bead was prepared by dropping the mixture thus prepared in a solution containing 5% (w / v) of calcium lactate by one drop. The diameter of the fabricated beads was about 0.6 to 0.8 cm.
제조된 비드를 젖산칼슘 용액으로부터 회수한 후, 세척하고, 냉장 보관하였다. The manufactured beads were recovered from the calcium lactate solution, washed, and refrigerated.
[[ 실시예Example 2: 본 발명 2: invention 농축유Concentrated oil 함유 캡슐을 포함한 음료의 제조] Containing capsules]
상기 실시예 1에서 제조한 본 발명 농축유 함유 캡슐을 커피에 넣어 본 발명 농축유 함유 캡슐을 포함하는 커피 음료를 제조하였다. 이때, 음료 용기의 옆에 직경 1cm 정도의 빨대를 붙여 음용자가 빨대를 통해 비드를 섭취할 수 있도록 하였다. The concentrate-containing capsules of the present invention prepared in Example 1 were put into coffee to prepare coffee beverages containing capsules containing the concentrate of the present invention. At this time, a straw having a diameter of about 1 cm was attached to the side of the beverage container so that the beverage could be taken by the user through the straw.
Claims (11)
상기 혼합액을 다가 양이온을 포함하는 용액에 떨어뜨려 캡슐을 제조하는 단계 (b); 및
상기 캡슐을 음료에 첨가하는 단계 (c);를 포함하며,
상기 캡슐은 우유의 지방 분해능이 있는 것을 특징으로 하는 옥수수수염물추출물 및 우유가 함유된 캡슐을 포함하는 음료의 제조방법.
Based on 25 ml of milk, 1.0 to 1.4 g of alginate, 0.6 to 1.0 g of glycerin, 0.05 to 0.15 g of potassium dihydrogenphosphate, 0.01 to 0.1 g of citric acid, 1.0 to 1.4 g of cornstarch water extract powder and 60 to 80 ml of water are added to milk (A) preparing a mixed solution by mixing and stirring;
(B) dropping the mixed solution into a solution containing a polyvalent cation to prepare a capsule; And
(C) adding the capsule to the beverage,
Wherein the capsule has a lipolytic ability of milk. ≪ RTI ID = 0.0 > 21. < / RTI >
상기 우유는,
농축유인 것을 특징으로 하는 옥수수수염물추출물 및 우유가 함유된 캡슐을 포함하는 음료의 제조방법.
The method according to claim 1,
Preferably,
A method for producing a beverage comprising a corn beer water extract and a milk-containing capsule, characterized in that the beverage is concentrated.
상기 알긴산염은,
알긴산나트륨인 것을 특징으로 하는 옥수수수염물추출물 및 우유가 함유된 캡슐을 포함하는 음료의 제조방법.
The method according to claim 1,
The alginate may be,
A method for producing a beverage comprising a corn steep liquor extract and a milk-containing capsule characterized by being sodium alginate.
상기 혼합액은,
중탕으로 가열하여 옥수수수염물추출물 분말과 알긴산염을 충분히 용해시키는 것을 특징으로 하는 옥수수수염물추출물 및 우유가 함유된 캡슐을 포함하는 음료의 제조방법.
The method according to claim 1,
The mixed liquid may contain,
A method for producing a beverage comprising a corn steep liquor extract and a milk-containing capsule, characterized in that the corn steep liquor is heated with a hot water to completely dissolve the corn steep liquor extract powder and alginate.
상기 단계 (a)는,
혼합액 제조 후, 우유향을 첨가하는 것을 특징을 하는 옥수수수염물추출물 및 우유가 함유된 캡슐을 포함하는 음료의 제조방법.
The method according to claim 1,
The step (a)
A method for producing a beverage comprising a corn steep liquor extract and a milk-containing capsule, characterized by adding a milk flavor after the preparation of the mixed liquid.
상기 다가 양이온을 함유하는 용액은,
젖산칼슘이 용해된 용액인 것을 특징으로 하는 옥수수수염물추출물 및 우유가 함유된 캡슐을 포함하는 음료의 제조방법.
The method according to claim 1,
The solution containing the polyvalent cation can be obtained by,
Wherein the calcium lactate is a solution in which calcium lactate is dissolved; and a capsule containing milk.
상기 젖산칼슘은,
용액 중 3~7%(w/v) 용해되어 있는 것을 특징으로 하는 옥수수수염물추출물 및 우유가 함유된 캡슐을 포함하는 음료의 제조방법.
9. The method of claim 8,
The calcium lactate,
Which comprises dissolving 3 to 7% (w / v) in a solution of corn steep liquor and a capsule containing milk.
상기 혼합액은,
다가 양이온을 포함하는 용액에 한 방울씩 떨어뜨리는 것을 특징으로 하는 옥수수수염물추출물 및 우유가 함유된 캡슐을 포함하는 음료의 제조방법.
The method according to claim 1,
The mixed liquid may contain,
A method for producing a beverage comprising a corn bean water extract and a milk-containing capsule, wherein the beverage is dropped by dropping into a solution containing a polyvalent cation.
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