KR101410791B1 - a fermentation medicinal herbs extract process - Google Patents
a fermentation medicinal herbs extract process Download PDFInfo
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- KR101410791B1 KR101410791B1 KR1020120094237A KR20120094237A KR101410791B1 KR 101410791 B1 KR101410791 B1 KR 101410791B1 KR 1020120094237 A KR1020120094237 A KR 1020120094237A KR 20120094237 A KR20120094237 A KR 20120094237A KR 101410791 B1 KR101410791 B1 KR 101410791B1
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- fermented
- fermenting
- lactic acid
- herbal medicines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
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- A—HUMAN NECESSITIES
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- A61K36/18—Magnoliophyta (angiosperms)
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- A—HUMAN NECESSITIES
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- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/302—Foods, ingredients or supplements having a functional effect on health having a modulating effect on age
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
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- A—HUMAN NECESSITIES
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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Abstract
본 발명은 발효 한약재 추출액의 제조방법에 관한 것으로서, 더욱 상세하게는 한약재(韓藥材)를 발효시킨 후 추출하여 한약재의 약리성(藥理性)을 증진시키고, 채내 섭취가 잘되며 항산화효과 등을 얻을 수 있도록 한 발명에 관한 것이다.
전술한 본 발명의 특징은, 생약재의 전체중량에 대해 0.3∼0.8중량%의 소금을 가하고 유산균을 부여하여 발효시킨 다음 가압 증류하여 획득한 추출물을 숙성 발효시키는 1 차 유산균 발효단계, 1차 유산균 발효물에 대해 0.1∼0.5 중량%의 수용성 프로폴리스를 가하고 유산균을 부여하여 발효시키는 2차 유산균 발효단계, 2차 유산균 발효물에 대해 0.5∼2 중량%의 미네랄을 가하고 효모균을 부여하여 발효시키는 3차 효모균 발효단계, 3차 효모균 발효물에 방선균·사상균·고초균·황국균·초산균을 접종하고 발효시키는 4차 발효단계, 4차 발효물에 대해 0.05∼1중량%의 글리세린을 첨가하고 고속 분산시켜 균질화시킨 다음 발효시키는 5차 발효단계에 의하여 복합 미생물 발효액을 완성하는 단계; 한약재를 0.7∼0.9%의 염도를 갖는 전해질수로 깨끗하게 세척하는 단계; 세척된 한약재를 건조실에 넣고 65∼80℃의 열풍을 부여하여 11∼13시간 건조시키는 단계; 건조된 한약재에 대해 2.5∼13중량%의 복합 미생물 발효액을 분무하여 48∼72시간 발효시키는 단계; 발효가 완료된 한약재를 물과 함께 탱크에 투입하여 내부온도가 35∼90℃가 유지되도록 하고, 물에 잠겨진 한약재에 초음파진동과 미세 기포를 부여하여 5∼7시간 추출하는 단계로 이루어진 것을 특징으로 하는 발효 한약재 추출액의 제조방법에 의하여 달성될 수 있는 것이다.The present invention relates to a method for producing an extract of a fermented medicinal herb, and more particularly, to a method for producing an extract of a fermented medicinal material, and more particularly, to a method for producing a fermented medicinal plant extract by fermenting and extracting a herbal medicinal material to enhance the pharmacological properties of the herbal medicines, The present invention relates to the invention.
The above-described characteristics of the present invention are as follows: a first lactic acid fermentation step of fermenting an extract obtained by adding 0.3 to 0.8 wt% of salt to the total weight of the herbal medicines, adding lactic acid bacteria to the lactic acid bacteria and fermenting the extract, A step of fermenting a second lactic acid bacterium to which 0.1 to 0.5 wt% of a water-soluble propolis is added to water and fermenting it by adding lactic acid bacteria; a step of adding 0.5 to 2 wt% of minerals to the fermented second lactic acid bacteria, A yeast fermentation step, a fourth fermentation step of inoculating and fermenting actinomycetes, filamentous fungi, Bacillus subtilis, Hwang gukyun, and acetic acid bacteria into a fermented product of a third yeast yeast, adding 0.05 to 1 wt% of glycerin to the fourth fermented product, Then completing the complex microbial fermentation broth by the fifth fermentation step; Washing the herbal medicines cleanly with electrolyte water having a salinity of 0.7-0.9%; Placing the washed herbal medicines in a drying chamber, applying hot air at 65 to 80 캜, and drying for 11 to 13 hours; Spraying the mixed microbial fermentation broth with 2.5 to 13 wt% of the dried herbal medicines and fermenting the mixture for 48 to 72 hours; The fermented herbal medicines are put into a tank together with water so that the internal temperature is maintained at 35 to 90 DEG C, and ultrasound vibration and fine bubbles are added to the herbal medicines immersed in water to extract them for 5 to 7 hours. And a method for producing an extract of a fermented medicinal material.
Description
본 발명은 발효 한약재 추출액의 제조방법에 관한 것으로서, 더욱 상세하게는 한약재(韓藥材)를 발효시킨 후 추출하여 한약재의 약리성(藥理性)을 증진시키고, 채내 섭취가 잘되며 항산화효과 등을 얻을 수 있도록 한 발명에 관한 것이다.
The present invention relates to a method for producing an extract of a fermented medicinal herb, and more particularly, to a method for producing an extract of a fermented medicinal material, and more particularly, to a method for producing a fermented medicinal plant extract by fermenting and extracting a herbal medicinal material to enhance the pharmacological properties of the herbal medicines, The present invention relates to the invention.
일반적으로 한의학(韓醫學)은 인간의 타고난 체질·기(氣)의 강약과 심성을 설명하고 개인의 성격과 감정의 수양·조절을 통하여 약재를 처방하여 질병을 예방 및 치료하는 학문으로 서양의학이 갖는 문제점의 대안으로 한의학이 각광 받고 있다.In general, Oriental medicine (Han medical science) describes the physical strength and mentality of human being, and prescribes medicines through the cultivation and control of personal personality and emotions to prevent and treat diseases. Oriental medicine is attracting attention as an alternative to the problem of having.
현대에는 한의학에서 치료의 근간이 되는 한약재의 조제 및 복용방법에 대한 연구가 꾸준히 진행되고 있고, 한약을 지을 때 사용되는 약재로는 갈근, 갈황, 계지, 감초, 결명자, 두충, 황기, 구기자, 나복자, 사삼, 인삼, 천궁, 황부자, 웅담, 오미자 등과 같이 다양한 종류가 있다.In the modern era, research on methods of preparing and taking medicinal herbs that are the basis of treatment in oriental medicine has been progressing steadily, and medicinal materials used in the preparation of Chinese medicine include, but are not limited to, , Ginseng, ginseng, celestial god, hwangbu, ugum, and omija.
그러나, 대부분의 한약재는 초본(草本)· 목본의 뿌리· 잎· 꽃 또는 열매로 이루어져 그대로 처방하거나 추출물을 약재로 사용하므로 환자가 복용하기에는 한약재의 향미(香味)가 나쁘고 체내 흡수가 원활하게 이루어지지 않아 소화기능에 나쁜 영향을 주는 등의 폐단이 발생되었다.However, most of herbal medicines are composed of roots, leaves, flowers, or fruits of herbaceous trees, and they are used as they are, and their extracts are used as medicines. Therefore, the herbal medicines have poor flavor and absorption Causing a negative effect on digestive function.
근래에 연구된 바에 의하면 한약재의 약용성분은 장내 미생물이 약용성분을 분해하여 대사물질(Metabolite)을 생성해야 체내 흡수가 잘되고, 약용성분 자체가 약효를 발휘하는 것이 아니라 몸에 흡수될 수 있도록 장내 미생물에 의해 분해된 대사물질이 약효물질이라 밝혀졌다.Recently, it has been studied that medicinal components of herb medicine are not easily absorbed into the body because the intestinal microorganisms decompose the medicinal components to produce the metabolic material, and the intestinal microbes The metabolites decomposed by the enzyme were found to be medicinal substances.
따라서, 환자가 장내 미생물이 부족한 경우에는 충분한 약효를 볼 수 없었고, 연구에 의하면 일반인의 35∼40%는 장내 미생물이 부족하여 약용성분을 충분히 흡수할 수 없다는 보고가 있다.Therefore, it has been reported that when the patient is lacking intestinal microorganisms, sufficient efficacy can not be obtained. According to the study, 35-40% of the general public can not sufficiently absorb the medicinal component due to lack of intestinal microorganisms.
이와 같이 체내 흡수력이 낮은 한약재를 단순하게 처방하거나 추출하여 복용하는 경우에는 한약재가 갖는 충분한 약효를 얻을 수 없었을 뿐 아니라 사용자의 소화 흡수력을 높여주거나 항산화효과를 증진시키는 등의 효과는 기대할 수 없었다.
In the case of simply prescribing or extracting herbal medicines with low absorption capacity in the body, not only the medicinal effects of herbal medicines can not be obtained, but also effects such as enhancing digestion absorption power of users or enhancing antioxidative effects can not be expected.
본 발명은 상기한 문제점을 감안하여 창안한 것으로서, 그 목적은 한약재(韓藥材)를 발효시킨 후 추출하여 채내 섭취가 잘되어 소화흡수력을 높여줄 수 있고, 항산화 효과 등을 얻을 수 있어 한약재의 약용효과를 극대화시킬 수 있는 발효 한약재 추출액의 제조방법을 제공함에 있는 것이다.
DISCLOSURE OF THE INVENTION The present invention has been made in view of the above problems, and an object of the present invention is to provide a medicinal herb medicine which can be obtained by fermenting a herbal medicine material, extracting it, And to provide a method for producing a fermented medicinal plant extract which can maximize its effect.
상기한 목적을 달성하기 위한 본 발명의 특징은, 생약재의 전체중량에 대해 0.3∼0.8중량%의 소금을 가하고 유산균을 부여하여 발효시킨 다음 가압 증류하여 획득한 추출물을 숙성 발효시키는 1 차 유산균 발효단계, 1차 유산균 발효물에 대해 0.1∼0.5 중량%의 수용성 프로폴리스를 가하고 유산균을 부여하여 발효시키는 2차 유산균 발효단계, 2차 유산균 발효물에 대해 0.5∼2 중량%의 미네랄을 가하고 효모균을 부여하여 발효시키는 3차 효모균 발효단계, 3차 효모균 발효물에 방선균·사상균·고초균·황국균·초산균을 접종하고 발효시키는 4차 발효단계, 4차 발효물에 대해 0.05∼1중량%의 글리세린을 첨가하고 고속 분산시켜 균질화시킨 다음 발효시키는 5차 발효단계에 의하여 복합 미생물 발효액을 완성하는 단계; 한약재를 0.7∼0.9%의 염도를 갖는 전해질수로 깨끗하게 세척하는 단계; 세척된 한약재를 건조실에 넣고 65∼80℃의 열풍을 부여하여 11∼13시간 건조시키는 단계; 건조된 한약재에 대해 2.5∼13중량%의 복합 미생물 발효액을 분무하여 48∼72시간 발효시키는 단계; 발효가 완료된 한약재를 물과 함께 탱크에 투입하여 내부온도가 35∼90℃가 유지되도록 하고, 물에 잠겨진 한약재에 초음파진동과 미세 기포를 부여하여 5∼7시간 추출하는 단계로 이루어진 것을 특징으로 하는 발효 한약재 추출액의 제조방법에 의하여 달성될 수 있는 것이다.
In order to accomplish the above object, the present invention provides a method for fermenting a primary lactic acid bacteria fermenting a fermented product by adding 0.3 to 0.8 wt% of salt to the total weight of the herbal medicines, adding lactic acid bacteria to the fermented product, , A step of fermenting a second lactic acid bacterium to which 0.1 to 0.5 wt% of a water-soluble propolis is added to the first lactic acid fermented product and fermenting the lactic acid bacterium to which the lactic acid bacterium is added, 0.5 to 2 wt% of minerals are added to the second lactic acid fermented product, A fourth fermentation step in which the fermented product of the third yeast strain is inoculated with fermented actinomycetes, filamentous fungi, germicidal bacteria, hwangguk gum, and acetic acid bacteria and fermented, and 0.05 to 1% by weight of glycerin is added to the fourth fermented product A step of homogenizing at high speed to complete a fermentation of the complex microorganism by a fifth fermentation step of fermenting; Washing the herbal medicines cleanly with electrolyte water having a salinity of 0.7-0.9%; Placing the washed herbal medicines in a drying chamber, applying hot air at 65 to 80 캜, and drying for 11 to 13 hours; Spraying the mixed microbial fermentation broth with 2.5 to 13 wt% of the dried herbal medicines and fermenting the mixture for 48 to 72 hours; The fermented herbal medicines are put into a tank together with water so that the internal temperature is maintained at 35 to 90 DEG C, and ultrasound vibration and fine bubbles are added to the herbal medicines immersed in water to extract them for 5 to 7 hours. And a method for producing an extract of a fermented medicinal material.
이상에서 상술한 바와 같이 본 발명의 제조과정에 의하여 완성된 발효 한약재 추출액은 복합 미생물 발효액으로 한약재를 발효시켜 사용한 것이므로 한약재의 향미(香味)가 좋으면서도 항산화효과를 증진시킬 수 있고, 발효에 의하여 약용성분이 충분히 분해되어 대사물질(Metabolite)이 생성된 상태에서 복용하므로 체내 흡수가 원활하게 이루어져 약용효과를 높여줄 수 있는 동시에 추출과정에서 미세 기포와 초음파진동에 의하여 한약재 내부까지 깊숙한 분해가 이루어져 효율적인 추출이 이루어지고 한약재가 갖는 고유 성분들의 약용효과를 더욱 높여주어 고품질의 한약재 추출액을 얻을 수 있을 뿐 아니라 발효 및 추출과정에서 한약재가 갖는 특수성분을 중화시키므로 체질에 관계없이 한약재의 약용 성분을 흡수하여 섭취할 수 있는 것으로서 대외 경쟁력이 우수한 고품질의 발효 한약재 추출액을 제공할 수 있는 등의 이점이 있다.
As described above, since the fermented herbal medicinal extract solution obtained by the manufacturing process of the present invention is used by fermenting the herb medicines with the fermented microbial fermentation broth of the present invention, it is possible to enhance the antioxidative effect while the flavor of the medicinal herb is good, (Metabolite) is formed in the body, so that it can be absorbed smoothly to improve the medicinal effect. At the same time, it is deeply decomposed to the inside of the herbal medicine by micro-bubbles and ultrasonic vibration during the extraction process, And the medicinal effect of the herbal ingredients of the medicinal herb is further enhanced to obtain a high quality medicinal herb extract as well as neutralizing the special ingredients of the medicinal herb during fermentation and extraction so that the medicinal ingredients of the medicinal herb are absorbed What you can do There are competitive advantages such as to provide an excellent quality of fermented herbal medicine extract.
도 1은 본 발명에 의한 발효 한약재 추출액의 제조과정을 순차적으로 나열한 제조공정도.FIG. 1 is a view showing a manufacturing process of a fermented medicinal herb extract according to the present invention, which is sequentially arranged.
이하, 상기한 목적을 달성하기 위한 바람직한 실시예를 첨부된 도 1의 제조 공정도에 의하여 상세히 설명하면 다음과 같다.DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
실시예Example
생약재의 전체중량에 대해 0.3∼0.8중량%의 소금을 첨가하고 미량의 유산균을 부여하여 30∼40℃의 온도를 유지하는 발효조에서 약 2일간 발효시킨 다음 가압 증류하여 추출물을 획득하고, 온도 30∼40℃의 온도를 유지하는 발효조에서 7∼10 일간 숙성 발효시키는 1차 유산균 발효단계를 실시하였다.0.3 to 0.8% by weight of salt was added to the total weight of the herbal medicine, and a small amount of lactic acid bacteria was added thereto. The fermented product was fermented in a fermenter maintained at a temperature of 30 to 40 캜 for about 2 days and then subjected to pressure distillation to obtain an extract. Fermentation step of fermenting primary fermented lactic acid fermented for 7 to 10 days in a fermenter maintained at a temperature of 40 ° C.
상기 "생약재"란 천연에서 얻어지는 의약품으로서, 천연으로 산출되는 자연물을 그대로 또는 말리던가, 썰거나 가루로 만드는 정도의 간단한 가공처리 한 것을 사용하였으며 참나무잎, 두송잎, 진피, 갈근, 갈화, 갈엽, 계피, 송순, 약쑥, 헛개나무 열매, 겨우살이, 조릿대, 까마중, 민들레, 인동꽃, 구기자, 및 이들의 조합으로 구성된 군에서 선택된 생약재를 사용하였다.The above-mentioned "herbal medicine" is a natural pharmaceutical product, which is a natural product produced by natural processes such as dried, cut or chopped, , A medicinal herb selected from the group consisting of Songsoon, Medicinal wormwood, Hovenia dulcis, Mistletoe, Sasae, Radish, Dandelion, Rhododendron, Gougi, and combinations thereof.
상기 유산균은 통상의 유산균 발효에 사용될 수 있는 임의의 유산균이 사용될 수 있으며, 한 종류의 유산균 또는 복합 유산균이 사용될 수 있다. 바람직하게는 락토바실러스 루테리, 락토바실러스 카제이, 락토바실러플란타룸, 락토바실러스 애시도필러스 및 이들의 조합을 사용하였다.The lactic acid bacteria may be any lactic acid bacteria that can be used for conventional lactic acid bacteria fermentation, and one kind of lactic acid bacteria or multiple lactic acid bacteria may be used. Preferably, Lactobacillus luteri, Lactobacillus casei, Lactobacillus lafflata, Lactobacillus acidophilus, and combinations thereof are used.
상기 가압증류는 증류장치에 대기압보다 높은 압력을 가하고 진행하는 증류 방법으로 당해 기술분에서 가압 증류에 의해 추출물 엑스트렉트(extract)를 획득하는 방법이 잘 알려져 있는 것이므로 본 발명에서는 증류방법의 상세한 설명은 생략하였다.The pressurized distillation is a well-known method for obtaining an extract of extract by pressurizing distillation by a distillation method in which a pressure higher than atmospheric pressure is applied to a distillation apparatus. Therefore, in the present invention, Is omitted.
이어서, 1차 유산균 발효물에 대해 0.1∼0.5 중량%의 수용성 프로폴리스를 가하고 유산균을 부여하여 30∼40℃의 온도를 유지하는 발효조에서 약 2일간 발효시키는 2차 유산균 발효단계를 실시하였다.Next, a fermentation step of a second lactic acid bacterium was carried out by adding 0.1 to 0.5% by weight of water-soluble propolis to the fermented product of the first lactic acid bacteria, adding lactic acid bacteria, and fermenting the fermented product in a fermenter maintained at a temperature of 30 to 40 ° C for about 2 days.
상기 수용성 프로폴리스는 상업적으로 판매되는 임의의 수용성 프로폴리스를 사용하였고, 수용성 프로폴리스는 그 자체가 항산화 효과를 갖는 물질이지만, 본 발명에 따른 상기 미생물 발효액은 프로폴리스의 단순한 부가에 의해 얻어지는 항산화 효과보다 현저히 높은 항산화 효과가 얻어질 수 있다.The water-soluble propolis may be any commercially available water-soluble propolis, and the water-soluble propolis itself may have an antioxidative effect. However, the microbial fermentation broth according to the present invention may have antioxidative effects obtained by simple addition of propolis A significantly higher antioxidant effect can be obtained.
이어서, 2차 유산균 발효물에 대해 0.5∼2 중량%의 미네랄을 가하고 효모균을 부여하여 30∼40℃의 온도를 유지하는 발효조에서 약 2일간 발효시키는 3차 효모균 발효단계를 실시하였다.Next, a fermentation step of the third yeast strain, in which 0.5-2 wt% of minerals were added to the fermented secondary lactic acid bacteria and yeast strains were added thereto and fermented in a fermenter maintained at a temperature of 30-40 ° C for about 2 days, was performed.
상기 "미네랄"은 인체의 성장과 유지 및 생식에 비교적 소량 필요한 영양물질인 광물질을 의미하며 칼슘, 인, 칼륨, 마그네슘, 황, 염소, 나트륨, 철, 아연, 구리, 망간, 요오드, 셀레늄, 몰리브덴, 코발트, 크롬 및 이들의 조합을 사용하였다.The term "mineral" means a mineral which is a nutrient which is relatively small in quantity for growth, maintenance and reproduction of the human body and includes calcium, phosphorus, potassium, magnesium, sulfur, chlorine, sodium, iron, zinc, copper, manganese, , Cobalt, chromium, and combinations thereof.
상기 효모균은 통상의 효모균 발효에 사용될 수 있는 임의의 효모균이 사용될 수 있으며 사카로마이세스 세레비지애(Saccharomyces cerevisiae), 사카로아미세스 엘리소이더스(Saccharomyces ellipsoidus) 또는 이들의 조합을 사용하였다.The yeast may be any yeast that can be used for conventional yeast fermentation, and Saccharomyces cerevisiae, Saccharomyces ellipsoidus, or a combination thereof is used.
이어서, 3차 효모균 발효물에 방선균·사상균·고초균·황국균 및 초산균을 접종하고 30∼40℃의 온도를 유지하는 발효조에서 약 2일간 발효시키는 4차 발효단계를 실시하였다.Next, a fourth fermentation step in which the actinomycetes, filamentous fungi, Bacillus subtilis, Hwang gukguk and acetic acid bacteria were inoculated into the fermentation product of the third yeast strain and fermented in a fermenter kept at a temperature of 30 to 40 캜 for about 2 days was carried out.
상기 방선균은 스트렙토마이세스 그리세우스(Streptomyces griseus), 스트렙토마이세스 아우레우스(Streptomyces aureus), 스트렙토마이세스 아비도플라부스(Streptomycesabisoflavus) 또는 이들의 조합을 사용하였다.The actinomycetes used Streptomyces griseus, Streptomyces aureus, Streptomyces abisoflavus, or a combination thereof.
상기 사상균은 누룩곰팡이속 (Aspergillus), 페니실리움속(Penicillium), 리조푸스속(Rhizopus)이 만들어낸 가는 실 모양의 균사체(菌絲體)와 자실체(子實體)를 사용하였다.The filamentous fungi used were filamentous fungi and fruiting bodies produced by Aspergillus, Penicillium, and Rhizopus.
상기 고초균은 바실러스 서브틸리스(Bacillus subtilis), 바실러스 서브틸리스 스피지제피아이(Bacillus subtilis), 바실러스 메가테리움(Bacillus megaterium), 바실러스 리체니포르미스(Bacillus licheniformis), 바실러스 풀미스(Bacillus pumilus) 또는 이들의 조합을 사용하였다.The Bacillus subtilis may be selected from the group consisting of Bacillus subtilis, Bacillus subtilis, Bacillus megaterium, Bacillus licheniformis, Bacillus pumilus, ) Or a combination thereof.
이어서, 4차 발효물에 대해 0.05∼1중량%의 글리세린을 첨가하고 고속 분산시켜 균질화시킨 다음 10∼30℃의 온도를 유지하는 발효조에서 약 2일간 발효시키는 5차 발효단계를 실시하여 복합 미생물 발효액을 완성하였다.Then, 0.05 to 1% by weight of glycerin was added to the fourth fermentation product, and homogenized at high speed and dispersed. Then, the fermentation was carried out in a fermenter maintained at a temperature of 10 to 30 캜 for about 2 days, .
상기 "글리세린"은 화학식 C3H5(OH)3, 분자량 92.10으로 흡습성이 강하고, 수용성이며, 자당의 3분의 1 정도의 감미가 있는 식품 첨가제이다.The above-mentioned "glycerin" is a food additive having the formula C3H5 (OH) 3, a molecular weight of 92.10, a hygroscopic property, water solubility and a sweetness about one-third of sucrose.
상기 고속분산 작업은 4차 복합 발효물과 글리세린의 혼합물을 균질화시키기 위해 수행하는 것으로서 800∼1000rpm의 속도로 회전시켜 균질화하였다.The high-speed dispersing operation was performed to homogenize a mixture of the fourth composite fermented product and glycerin, and was homogenized by rotating at a speed of 800 to 1000 rpm.
상기 5차 발효에 의하여 완성된 복합 미생물 발효액은 그 자체로서 항산화 효과가 매우 뛰어날 뿐만 아니라 섭취하였을 때 체내 효소작용에 도움을 주며, 음식을 섭취 후 음용하면 소화 흡수를 촉진시키고, 일반적인 장내 부패균의 병원성 세균의 증식을 억제하는 효과가 있다.The complex microbial fermentation broth completed by the fifth fermentation is not only excellent in antioxidative effect itself but also contributes to the enzymatic action when ingested and promotes digestion and absorption by ingesting food after ingestion, It has an effect of inhibiting the proliferation of bacteria.
이어서, 한약재를 0.7∼0.9%의 염도를 갖는 전해질수로 깨끗하게 세척하는 단계를 실시하였다.Then, the herbal medicines were washed cleanly with an electrolyte water having a salinity of 0.7 to 0.9%.
상기 "한약재"는 천연에서 얻어지거나 재배한 것으로서, 한약재를 그대로 또는 말리던가, 썰거나 가루로 만드는 정도의 간단한 가공처리 한 것을 사용하였으며 갈근, 갈황, 계지, 감초, 결명자, 두충, 황기, 구기자, 나복자, 사삼, 인삼, 천궁, 황부자, 웅담, 오미자, 하수오, 복령, 달맞이씨, 까마중, 숙지황, 용안육, 오디, 시호, 한련초, 복분자, 계내금, 당귀, 진핀, 연자육, 산수유, 산약 및 이들의 조합으로 구성된 군에서 선택된 한약재를 사용하였다.The above-mentioned "herbal medicine material" is obtained from natural or cultivated material, and the herbal medicine material is used as it is, or a simple processing such as drying, cutting or powdering is used. , Ginseng, ginseng, celestial god, hwangbu, ugum, omija, hwaseo, gyeongryeong, mooncake, mammae, Were used. ≪ tb > < TABLE >
이어서, 세척된 한약재를 건조실에 넣고 65∼80℃의 열풍을 부여하여 내부 공기를 대류(對流)시키면서 11∼13시간 건조시키는 단계를 실시하였다.Then, the washed medicinal herb was placed in a drying chamber, and a hot air of 65 to 80 캜 was applied to the inside air to be convected while being dried for 11 to 13 hours.
이어서, 상기 제조과정에서 의하여 완료된 복합 미생물 발효액을 건조된 한약재에 분무하여 48∼72시간 발효시키는 단계를 실시하였다. 이때, 복합 미생물 발효액의 양은 건조된 한약재에 대해 2.5∼13중량%가 되도록 하였다.Then, the composite microbial fermentation solution was sprayed on the dried herbal medicines and fermented for 48 to 72 hours. At this time, the amount of the mixed microbial fermentation broth was adjusted to 2.5 to 13 wt% with respect to the dried medicinal herb.
이어서, 발효가 완료된 한약재를 물과 함께 탱크에 투입하였고, 물은 한약재 전체중량의 5∼8배가 되도록 하였으며, 한약재는 직물 주머니에 넣고 용수에 투입하여 추출작업 후 찌꺼기의 처리가 용이하게 이루어지도록 하였다.Then, the fermented medicinal herb was added to the tank together with water, the water was adjusted to 5 to 8 times the total weight of the medicinal herb, and the medicinal herb was put into a woven bag and put into water to facilitate the treatment of the remnant after the extraction .
이어서, 한약재와 물이 투입된 탱크를 히터로 가열하여 내부온도가 35∼90℃가 유지되도록 하고, 탱크 내부의 기압은 200∼500mmHg가 유지되도록 하여 5∼7시간 추출하는 단계를 실시하였다.Then, the tank containing the herb medicine and the water was heated with a heater to maintain the internal temperature at 35 to 90 DEG C, and the pressure inside the tank was maintained at 200 to 500 mmHg, thereby performing extraction for 5 to 7 hours.
상기 추출과정에서 탱크 내부에 초음파진동과 미세 기포를 부여하여 한약재의 내부 깊숙한 분해가 이루어지고 추출효과를 높일 수 있도록 하였으며, 추출 중에 수증기는 계속 배출되어 한약재의 약용성분이 농축된 엑기스 형태의 추출액을 얻을 수 있는 것이다.In the extraction process, ultrasonic vibration and fine bubbles are given to the inside of the tank to deeply decompose the herb medicines and increase the extraction effect. During the extraction, water vapor is continuously discharged and the extract of the herbal medicine concentrates You can get it.
상기 초음파진동은 탱크의 하부에 압전체로 구성된 초음파진동자를 설치하여 진동자에서 발산되는 초음파의 진동이 용수(用手)를 통해 한약재에 전달되어 분해효과를 높여주고 약용성분의 정밀 추출이 이루어진다.In the ultrasonic vibration, an ultrasonic vibrator composed of a piezoelectric body is installed in the lower part of the tank, and the vibration of the ultrasonic wave emitted from the vibrator is transmitted to the medicinal herb through the water to improve the decomposition effect and precise extraction of the medicinal component is performed.
상기 미세 기포는 유체속으로 유입되는 공기를 미세기포로 변환시키는 임펠러의 구동에 의하여 50㎛ 이하의 미세기포를 발생시키는 마이크로 버블(Micro bubble) 발생기를 사용하였으며 미세 기포의 발생은 용존 공기 부상법(DAF)을 이용하였다.The micro bubble is a micro bubble generator which generates fine bubbles of 50 탆 or less by driving an impeller which converts air introduced into a fluid into a micro bubble. The micro bubble is generated by the air- ) Were used.
상기 미세 기포는 한약재의 깊숙한 분해가 이루어지도록 하고, 용수(容手)의 산소포화농도를 증가시켜 수질을 개선하여 고품질의 추출액을 얻을 수 있도록 하는 역할을 한다.The microbubbles serve to decompose the herbal medicines deeply and to improve the water quality by increasing the oxygen saturation concentration of the water, thereby obtaining a high-quality extract.
상기 추출단계에 의하여 한약재의 약용효과가 높고, 항산화 효과가 증진되는 고품질의 발효 한약재 추출액을 얻을 수 있었으며, 한약재가 담겨진 주머니를 건지고 추출액을 침전시켜 상등 액을 약재(藥材)로 사용하였다.By the above extraction step, a high-quality fermented medicinal herb extract solution having high medicinal effect of herbal medicines and high antioxidative effect was obtained. The pouch containing herbal medicines was drained and the extract solution was precipitated, and the supernatant was used as a medicament.
전술한 제조과정에 의하여 완성된 발효 한약재 추출액은 생약재를 5차에 거쳐 발효시킨 복합 미생물 발효액으로 한약재를 발효시켜 사용한 것이므로 한약재의 향미(香味)가 좋으면서도 항산화효과를 증진시킬 수 있고, 발효에 의하여 약용성분이 충분히 분해되어 대사물질(Metabolite)이 생성된 상태에서 복용하므로 체내 흡수가 원활하게 이루어져 약용효과를 더욱 높여줄 수 있는 동시에 추출과정에서 미세 기포와 초음파진동에 의하여 한약재 내부까지 깊숙한 분해가 이루어져 효율적인 추출이 이루어지고 추출과정이 활성화되는 것이므로 한약재가 갖는 고유 성분들의 약용효과를 더욱 높여주어 고품질의 한약재 추출액을 얻을 수 있는 것으로서 대외 경쟁력이 우수한 발효 한약재 추출액을 제공할 수 있는 것이다.The fermented herbal medicinal extract solution obtained by the above-mentioned manufacturing process is fermented by the fermentation of the herbal medicines with the fermented microorganism fermented product obtained by fermenting the herbal medicines through the 5th step. Therefore, the antioxidative effect can be enhanced while the herbal taste of the medicinal herb is good, Since the medicinal ingredient is sufficiently decomposed to take the medicament in a state where the metabolite is produced, absorption into the body can be smoothly performed, thereby further enhancing the medicinal effect. At the same time, the microbubble and ultrasonic vibration deeply decompose to the inside of the medicinal herb It is possible to provide an extract of a fermented medicinal herb having superior external competitiveness because it is possible to obtain an extract of a medicinal herb of high quality by further enhancing the medicinal effect of the inherent ingredients of the medicinal herb.
또한, 한약재가 밀폐된 탱크의 내부에서 약 40℃의 저온에서 추출하는 것이므로 휘발성이 강한 약용성분의 손실을 최소화시킬 수 있고, 필요 이상의 고열이 가해질 우려가 없어 한약재 고유의 맛과 향미(香味)를 유지할 수 있는 동시에 충분한 열분해와 활성화작용으로 기존에 존재하지 않던 새로운 유효성분들이 생성됨과 아울러 기존성분들의 함량 증가로 인해 약용효과를 더욱 향상시킬 수 있는 것이다.In addition, since the herbal medicines are extracted at a low temperature of about 40 캜 in a sealed tank, the loss of the volatile medicinal ingredient can be minimized, and there is no fear that a high heat is unnecessarily applied. And at the same time, sufficient pyrolysis and activation action can produce new effective ingredients that have not existed before, and the medicinal effect can be further improved by increasing the content of existing components.
한편, 발효가 완료된 한약재를 물과 함께 탱크에 투입하고, 초음파진동과 미세 기포를 부여하여 추출하는 단계에서 증발되는 증기를 냉각기를 통과시키면서 32∼36시간 응축하여 증류액을 포집하는 단계가 더 포함되도록 할 수도 있다.Meanwhile, the step of adding the fermented medicinal herb to the tank together with water, and adding the ultrasound vibration and microbubbles to extract the steam, the steam is condensed for 32 to 36 hours while passing through the cooler to collect the distillate .
상기 냉각기는 탱크의 상부에 접속된 배출관이 나선형으로 감겨지고, 나선형 배출관이 냉수(冷水)가 순환되는 냉각통의 내부에 위치되어 증기가 응축되면서 낙하되면 증류액을 포집하는 시스템으로 구성되어 있고, 여기서 포집된 증류액은 각종 화장품의 원료로 사용된다.The cooler includes a system in which a discharge pipe connected to an upper part of a tank is spirally wound and a helical discharge pipe is located inside a cooling cylinder in which cold water is circulated and the distillate is collected when the steam falls and is condensed. The distillate collected here is used as a raw material for various cosmetics.
이상에서는 본 발명의 바람직한 실시예에 대하여 도시하고 또한 설명하였으나, 본 발명은 상기한 실시예에 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 본 발명이 속하는 분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형 실시가 가능한 것은 물론이고, 그와 같은 변경은 기재된 청구범위 내에 있게 된다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but many variations and modifications may be made without departing from the scope of the invention. It will be understood by those skilled in the art that various changes may be made and equivalents may be resorted to without departing from the scope of the appended claims.
Claims (4)
갈근, 갈황, 계지, 감초, 결명자, 두충, 황기, 구기자, 나복자, 사삼, 인삼, 천궁, 황부자, 웅담, 오미자, 하수오, 복령, 달맞이씨, 까마중, 숙지황, 용안육, 오디, 시호, 한련초, 복분자, 계내금, 당귀, 진핀, 연자육, 산수유, 산약 및 이들의 조합으로 구성된 군에서 선택된 한약재를 0.7∼0.9%의 염도를 갖는 전해질수로 깨끗하게 세척하는 단계;
세척된 한약재를 건조실에 넣고 65∼80℃의 열풍을 부여하여 11∼13시간 건조시키는 단계;
건조된 한약재에 대해 2.5∼13중량%의 복합 미생물 발효액을 분무하여 48∼72시간 발효시키는 단계;
발효가 완료된 한약재를 물과 함께 탱크에 투입하여 내부온도가 35∼90℃가 유지되도록 하고, 물에 잠겨진 한약재에 초음파진동과 미세 기포를 부여하여 5∼7시간 추출하는 단계로 이루어진 것을 특징으로 하는 발효 한약재 추출액의 제조방법.
The total weight of the herbal medicines selected from the group consisting of oak leaves, sheep leaves, dermis, pupae, garnet, lobules, cinnamon, trout, wormwood, hovenous fruit, mistletoe, sage, rape, dandelion, A fermentation step of fermenting the fermented product by adding 0.3 to 0.8 wt% of salt to the fermented lactic acid bacteria and fermenting the fermented product, and then fermenting the fermented product to ferment the primary fermented lactic acid fermented product; 0.1 to 0.5 wt% Fermenting a second lactic acid bacteria fermented by adding lactic acid bacteria to the second lactic acid bacteria fermented product, adding a 0.5-2 wt% mineral to the second lactic acid fermented product, adding the yeast and fermenting the third yeast, fermenting the third yeast yeast, The fourth fermentation step is a fourth fermentation step of inoculating and fermenting Bacillus subtilis, Hwang Kookun gum, and acetic acid bacteria, 0.05 to 1 wt% of glycerin to the fourth fermentation product, That following completion of the fermentation step of the composite microbial fermentation broth, by the fifth step of the fermentation;
Ginseng, ginseng, ginseng, hwangbu, ugum, omija, hwaseo, bokryeong, mooncake, mackerel, gyeolgyori, yongcheng, audi, shiho, hansikcho, bokbunja , Gold in the body, Angelica keiskei, Jinpin, pomegranate juice, corn oil, sunscreen, and combinations thereof, with an electrolyte water having a salinity of 0.7 to 0.9%;
Placing the washed herbal medicines in a drying chamber, applying hot air at 65 to 80 캜, and drying for 11 to 13 hours;
Spraying the mixed microbial fermentation broth with 2.5 to 13 wt% of the dried herbal medicines and fermenting the mixture for 48 to 72 hours;
The fermented herbal medicines are put into a tank together with water so that the internal temperature is maintained at 35 to 90 DEG C, and ultrasound vibration and fine bubbles are added to the herbal medicines immersed in water to extract them for 5 to 7 hours. A method for producing a fermented herbal medicine extract.
발효가 완료된 한약재를 물과 함께 탱크에 투입하고, 초음파진동과 미세 기포를 부여하여 추출하는 단계에서 증발되는 증기를 냉각기를 통과시키면서 32∼36시간 응축하여 증류액을 포집하는 단계가 더 포함되도록 한 것을 특징으로 하는 발효 한약재 추출액의 제조방법.
The method according to claim 1,
Adding the fermented medicinal herb to the tank together with water, and adding the ultrasonic vibration and microbubbles to extract the steam, the steam is condensed for 32 to 36 hours while passing through the cooler to collect the distillate ≪ / RTI > wherein the method comprises the steps of:
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KR101634670B1 (en) * | 2015-07-27 | 2016-06-30 | 강원대학교산학협력단 | Cosmetic composition comprising fermented extract of mistletoe for anti-inflammation or anti-allergy |
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KR102235856B1 (en) * | 2018-10-23 | 2021-04-05 | 고창군 | Fermentation beverage composition of rubus coreanus containing pine bud |
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