KR101237039B1 - Cultivating system for phytoplankton - Google Patents

Cultivating system for phytoplankton Download PDF

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Publication number
KR101237039B1
KR101237039B1 KR1020100123227A KR20100123227A KR101237039B1 KR 101237039 B1 KR101237039 B1 KR 101237039B1 KR 1020100123227 A KR1020100123227 A KR 1020100123227A KR 20100123227 A KR20100123227 A KR 20100123227A KR 101237039 B1 KR101237039 B1 KR 101237039B1
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South Korea
Prior art keywords
culture vessel
phytoplankton
filter
sea water
stirring
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KR1020100123227A
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Korean (ko)
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KR20120062118A (en
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정승원
김영옥
조성록
김시욱
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한국해양과학기술원
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/56—Floating elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention includes a culture vessel for culturing phytoplankton filled with phytoplankton and pure sea water, a filter unit for supplying pure seawater to the culture vessel, and a stirring unit for stirring the inside of the culture vessel, wherein the culture vessel floats in the sea. It is installed in a flotation device, and part of it is arranged to be submerged in sea water, and configured to match the environment of the sea and the environment inside the culture vessel, can improve the dryness of phytoplankton and reduce the cost of culture.

Description

Phytoplankton cultivation system {CULTIVATING SYSTEM FOR PHYTOPLANKTON}

The present invention relates to a phytoplankton culture apparatus capable of culturing microphytoplankton in large quantities.

In recent years, due to functional diversity, interest in microphytoplankton such as photosynthetic microorganisms and microalgae has been increasing, and the scope of research in various fields has been widened. As microphytoplankton possesses photosynthetic capacity, it is actively used for research on carbon dioxide reduction, which has been of great interest recently due to environmental problems such as global warming, and it is noted as a sustainable energy source for depletion of fossil fuels. It is being used for research related to the production of bioenergy such as biodiesel, bioethanol and hydrogen gas.

However, microphytoplankton cultivation must be carried out on a large scale and at high concentrations for the removal of quantitatively meaningful carbon dioxide using microphytoplankton or for the mass production of useful products such as bioenergy. Therefore, a situation in which technology related to the construction of a large-scale culture facility is required.

Currently, various types of photobioreactors installed indoors on land are used as a cultivation facility for culturing microphytoplankton.

As such, the cultivation facilities installed indoors on land should be equipped with artificial lighting means, and the temperature control facilities must be provided to maintain the temperature of the cultivation facilities appropriately. need.

In addition, the cultivation facilities installed indoors of the land is not only expensive to enlarge the scale, but also requires space constraints due to the need for a large space.

The present invention is to provide a phytoplankton culture apparatus that can maintain the health by culturing phytoplankton directly in the sea.

In addition, the present invention is to provide a phytoplankton cultivation apparatus that can be cultured in large quantities by directly culturing phytoplankton as a biofuel in the sea to reduce the cost of culture.

In addition, the present invention is to provide a phytoplankton culture apparatus capable of culturing phytoplankton directly in the sea to be rapidly cultured if necessary.

The technical problem to be achieved by the present invention is not limited to the technical problem mentioned above, and other technical problems not mentioned above may be clearly understood by those skilled in the art from the following description. will be.

In one embodiment, the phytoplankton incubator includes a culture vessel for culturing phytoplankton filled with phytoplankton and pure sea water, a filter unit for supplying pure sea water to the culture vessel, and a stirring unit for stirring the inside of the culture vessel, The culture vessel is installed in a flotation device floating in the sea, it is characterized in that the part is arranged to be submerged in sea water is configured to match the environment of the sea and the environment inside the culture vessel.

The culture vessel according to an embodiment includes a frame forming a cylindrical shape, a culture vessel fixed to the frame and open at an upper side thereof, and a cover member installed on an upper surface of the frame to cover an upper surface of the culture vessel, wherein the culture vessel Between the top and the upper frame of the air passage for allowing the outside air is characterized in that it is provided.

Filter unit according to an embodiment includes a plurality of filters to remove the microorganisms contained in the sea water by filtering the sea water multiple times, and a pump for pumping sea water,

The filter includes a first filter for filtering seawater to 50 μm, a second filter for filtering the first filtered sea water to 30 μm, a third filter for filtering the second filtered sea water to 3 μm, and a third order filtering And a fourth filter for filtering the seawater once again to 3 μm.

The stirring unit according to an embodiment includes a stirring blade inserted into the culture vessel, a rotating shaft to which the stirring blade is fixed, a driving motor installed on a support disposed above the culture vessel to drive the rotating shaft, and the driving. It includes a control box that controls the motor.

Phytoplankton cultivation apparatus according to an embodiment of the present invention is a culture vessel is installed in the sea and a portion of the culture vessel is arranged to be submerged in sea water to maintain the health of the culture organism by matching the environment of the culture vessel and the environment of the sea There is an advantage.

In addition, the culture apparatus is installed in the sea, there is no need to artificially create the sea environment has the advantage of reducing the cost of culture.

In addition, since a plurality of culture apparatus is installed in the sea there is an advantage that can be mass culture.

1 is a perspective view of a culture vessel according to an embodiment of the present invention.
Figure 2 is a block diagram showing a state in which the culture vessel according to an embodiment of the present invention is installed in the sea.
3 is a block diagram of a filter unit according to an embodiment of the present invention.
Figure 4 is a cross-sectional view of the culture vessel showing a stirring device according to an embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. The sizes and shapes of the components shown in the drawings may be exaggerated for clarity and convenience. In addition, terms defined in consideration of the configuration and operation of the present invention may be changed according to the intention or custom of the user, the operator. Definitions of these terms should be based on the content of this specification.

1 is a block diagram of a phytoplankton culture vessel according to an embodiment of the present invention.

Phytoplankton cultivation apparatus according to an embodiment of the present invention is a culture vessel (10) which is partially submerged in the sea, the filter unit 50 for producing pure sea water filled in the culture vessel 10, and the culture vessel (10) a stirring device 80 for stirring the inside.

The culture apparatus according to the present embodiment is installed in the sea to match the environment of the sea and the environment of the culture vessel to improve the cultivation capacity of phytoplankton.

Therefore, the culture vessel 10 is arranged in plurality in the flotation device 100 is installed in the state floating in the sea, a portion of the culture vessel 10 is arranged to be submerged in the sea temperature of the sea water and the culture vessel 10 inside Allow the temperature to match.

As shown in FIG. 2, the culture vessel 10 is formed in a tubular shape to support the entire culture vessel 10, and is fixed to the frame 20 to fill the culture material and pure seawater. The lid 30, which is mounted on the culture vessel 30 and the upper surface of the frame 20, passes air into the culture vessel 30 and blocks foreign substances or rainwater from entering the culture vessel 30. Include.

The frame 20 has a ring-shaped upper frame 22, a side frame 24 extending perpendicular to the upper frame 22, and a ring-shaped lower frame 26 connected to the lower side of the side frame 24. ).

The culture vessel 30 has a cylindrical shape with an upper side open, the upper end is fixed to the upper frame 22, the lower end is fixed to the lower frame 26. The culture vessel 30 is formed of a vinyl material, for example, poly ethanol material, so that the manufacturing and installation is easy and the thermal conductivity inside and outside the culture vessel 30 can be easily.

The culture apparatus according to the present embodiment is mainly installed in the sea so that the phytoplankton can be cultured directly in the sea so that the sea environment and the culture environment can be matched to improve the health of the cultured organisms.

In the sea environment, in particular, the temperature of the sea water and the temperature inside the culture vessel 30 should match each other, since the thermal conductivity of the culture vessel 30 is made of poly ethanol well, the temperature of the sea water and the temperature inside the culture vessel 30 Can be quickly matched and the temperature of the seawater and the temperature inside the vessel can be kept consistent during incubation.

An air passage 32 is provided between the upper frame 22 and the upper surface of the culture vessel 30 to allow outside air to freely enter and exit the culture vessel 30.

That is, the upper surface of the culture vessel 30 is fixed in a manner of weaving the wire 34 to the upper frame 22, in which the space between the culture vessel 30 and the upper frame 22 by loosening the wire 34 Formed, and this space serves as an air passage.

The lid member 40 is formed on a top surface of the upper frame 22 and is formed of a transparent material through which light can pass, and a hemispherical dome portion 42 is formed at the center thereof.

The lid member 40 covers the upper surface of the upper frame 22 and serves to block foreign matter or rainwater from flowing into the culture vessel 30, and a dome portion 42 is formed at the center to provide sufficient sunlight. It is to be supplied into the culture vessel (30).

The filter unit 50 is for manufacturing pure seawater from which various foreign matters and microorganisms are contained in the seawater, and the pump 52 pumping the seawater and the seawater pumped by the pump 52 are sequentially filtered. A plurality of filters 60, 62, 64, 66 are included.

The filter comprises a first filter 60 for first filtering the seawater, a second filter 62 for secondarily filtering the seawater filtered through the first filter 60, and a second filter 62. A third filter 64 for tertiary filtering the second filtered seawater while passing through, and a fourth filter 66 disposed at the rear side of the pump 52 to finally filter the seawater flowing into the culture vessel 30. It includes.

Here, the first filter 60 filters the seawater to 50 μm, and the second filter 62 filters the first filtered sea water to 30 μm. The third filter 64 filters the second filtered seawater to 3 μm, and the fourth filter 66 finally filters the third filtered sea water to 3 μm again and supplies it to the culture vessel 30. do.

The first filter 60 is connected to the seawater suction line 72, the filters are connected to the connection line 74, and the fourth filter 66 is connected to the seawater discharge line 76, and the seawater discharge line. 76 is connected to the culture vessel 30.

The pump 52 may be installed at any one of the connection lines 74. In this embodiment, the pump 52 is installed in the connection line connecting the third filter and the fourth filter to pump sea water.

As shown in FIG. 4, the stirring device 80 is inserted into the culture vessel 30 to stir the inside of the culture vessel 30, and the stirring blade 84 and the stirring wing 84 are fixed to the culture vessel ( A rotation shaft 86 disposed vertically on the upper side of the 30 and a driving motor fixed to the rotation shaft 86 and driving the rotation shaft 86 and installed on a support 82 disposed above the culture vessel 30. 88, and a control box 90 for controlling the drive motor 88.

The stirring device 80 serves to evenly mix the pure sea water and the culture material (plant plankton) inside the culture vessel (30).

The stirring blade 84 is one or more arranged on the rotary shaft 86.

Support 82 is formed in the form of a flat plate that is fixed to both ends of the upper frame, the guide member 92 for supporting the rotating shaft 86 is fixed to one side of the support to prevent shaking of the rotating shaft 86, this At the end of the guide member 92, a support plate 94 for rotatably supporting the rotation shaft 86 is fixed.

The control box 90 adjusts the stirring speed by controlling the speed of the driving motor 88, and a timer is provided to adjust the operating time and the operating frequency of the driving motor.

Thus, the operation of the phytoplankton culture apparatus according to an embodiment of the present invention is described below.

A portion of the culture vessel 10 is disposed to be immersed in sea water to maintain the temperature of the sea water and the internal temperature of the culture vessel 10 to improve the culture performance.

In particular, when culturing on land requires a temperature control device for temperature control of the culture vessel, but the culture vessel according to the present embodiment can be installed in the sea so that a separate temperature control device is not necessary, thereby reducing the cost.

And, because the culture vessel 10 is installed in the sea can match the sea environment and the culture environment can maintain the health of the cultured organisms.

Filter unit 50 is provided to filter the sea water can be produced pure sea water and to supply the pure sea water to the culture vessel can improve the work efficiency.

In addition, the stirring speed, the stirring time, and the number of stirring times of the stirring device 80 can be automatically adjusted, so that once the setting is agitated, the culture performance is improved and the cultivation work is convenient.

While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. Accordingly, the true scope of the present invention should be determined by the following claims.

10: culture vessel 20: frame
22: upper frame 24: side frame
26: lower frame 30: culture vessel
32: air passage 40: cover member
42: dome 50: filter unit
52: pump 60: first filter
62: second filter 64: third filter
66: fourth filter 80: stirring device
82: support 84: stirring blade
86: shaft 88: drive motor
90: control box

Claims (8)

It comprises a culture vessel for culturing phytoplankton filled with phytoplankton and pure sea water, a filter unit for supplying pure sea water to the culture vessel, and a stirring unit for stirring the inside of the culture vessel,
The culture vessel is installed in the flotation device floating in the sea, a portion of which is placed soaked in sea water to match the environment of the sea and the environment inside the culture vessel,
The culture vessel includes a frame having a cylindrical shape, a culture vessel fixed to the frame and open at an upper side thereof, and a cover member installed on an upper surface of the frame to cover the upper surface of the culture vessel,
Between the top of the culture vessel and the upper frame is provided with an air passage for the outside air to enter,
The culture vessel is phytoplankton cultivation apparatus, characterized in that formed of a polyethanol material that can be easily transformed thermal conductivity inside and outside the culture vessel. delete delete The method of claim 1,
The cover member is in the form of a transparent disc, phytoplankton incubator is formed in the center of the dome portion protruding in the form of hemispheres to collect sunlight. The method of claim 1,
The filter unit includes a plurality of filters for filtering the sea water multiple times to remove microorganisms contained in the sea water, and a pump for pumping the sea water,
Said filter is arranged in plural phytoplankton incubator, characterized in that to filter the sea water in multiple stages. The method of claim 5,
The filter includes a first filter for filtering seawater to 50 μm, a second filter for filtering the first filtered sea water to 30 μm, a third filter for filtering the second filtered sea water to 3 μm, and a third order filtering Phytoplankton culture apparatus comprising a fourth filter for filtering the seawater once again to 3㎛. The method of claim 1,
The stirring unit includes a stirring blade inserted into the culture vessel, a rotating shaft to which the stirring blade is fixed, a driving motor installed on a support disposed above the culture vessel for driving the rotating shaft, and controlling the driving motor. Phytoplankton incubator comprising a control box. The method of claim 7, wherein
The control box controls the driving motor to control the stirring speed, and a timer is provided phytoplankton incubator to adjust the stirring time and the number of stirring times.
KR1020100123227A 2010-12-06 2010-12-06 Cultivating system for phytoplankton KR101237039B1 (en)

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KR1020100123227A KR101237039B1 (en) 2010-12-06 2010-12-06 Cultivating system for phytoplankton

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KR101237039B1 true KR101237039B1 (en) 2013-02-25

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101412138B1 (en) 2013-03-28 2014-06-25 현대제철 주식회사 Culture apparatus for water flea
KR101869770B1 (en) 2017-06-28 2018-06-22 대한민국 Device for field cultivating algae

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101640674B1 (en) * 2014-11-27 2016-07-18 주식회사 지디이 Humidification type culture apparatus

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100903691B1 (en) * 2008-09-12 2009-06-18 한국농어촌공사 Cultivation apparatus of living organism to cause retide and its predator
US20100255569A1 (en) * 2009-04-02 2010-10-07 Camarate De Albuquerque Maranhao Andre Algae photobioreactor
KR100991373B1 (en) * 2008-12-03 2010-11-02 인하대학교 산학협력단 Photobioreactor for marine microalgal mass cultures using permeable menbrane

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100903691B1 (en) * 2008-09-12 2009-06-18 한국농어촌공사 Cultivation apparatus of living organism to cause retide and its predator
KR100991373B1 (en) * 2008-12-03 2010-11-02 인하대학교 산학협력단 Photobioreactor for marine microalgal mass cultures using permeable menbrane
US20100255569A1 (en) * 2009-04-02 2010-10-07 Camarate De Albuquerque Maranhao Andre Algae photobioreactor

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101412138B1 (en) 2013-03-28 2014-06-25 현대제철 주식회사 Culture apparatus for water flea
KR101869770B1 (en) 2017-06-28 2018-06-22 대한민국 Device for field cultivating algae

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