KR100259326B1 - Biological control of ginseng disease and a novel of antagonistic bacteria therefor - Google Patents

Biological control of ginseng disease and a novel of antagonistic bacteria therefor Download PDF

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KR100259326B1
KR100259326B1 KR1019980015406A KR19980015406A KR100259326B1 KR 100259326 B1 KR100259326 B1 KR 100259326B1 KR 1019980015406 A KR1019980015406 A KR 1019980015406A KR 19980015406 A KR19980015406 A KR 19980015406A KR 100259326 B1 KR100259326 B1 KR 100259326B1
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ginseng
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soil
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유성준
김선익
김홍기
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유성준
김홍기
김선익
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Abstract

PURPOSE: Provided is a novel antagonist bacteria, Bacillus sp. 16P5 (KCTC 8887P), which has high affinity with ginseng, viability in a wide range of pH and low nutrient, and antagonisity to ginseng diseases to be used in manufacturing farm chemicals. CONSTITUTION: Bacillus sp. 16P5 (KCTC 8887P) is obtained by the following steps of: i) selecting 7 kinds of pathogenic bacteria from soil, which are as follows: Cylindrocarpon destructans, Fusarium solani, Rhisoctonia solani, Botrytis cinerea, Phytophthora cactorum, Pythium sp., and Alternaria panax; ii) selecting an antagonist bacteria strain showing antagonisity to other 6 kinds of bacteria; and iii) identifying the bacteria as Bacillus sp. 16P5 strain and measuring requirements for cultivation of the bacteria.

Description

인삼 연작장해의 생물학적 방제방법 및 그에 유용한 신규한 길항세균Biological Control Method of Ginseng Serial Injury and Novel Antagonistic Bacteria

본 발명은 인삼의 연작장해의 생물학적 방제방법에 관한 것이다. 더욱 상세하게는, 본 발명은 인삼의 연작에 따른 토양균에 의해 발생하는 각종 인삼질병의 방제에 유용한 길항세균을 제공함에 있다. 더욱 상세하게는, 본 발명은 여러 병원균들에 대해 종합적으로 높은 길항력을 갖고 인삼에 대한 높은 친화성을 가지며 넓은 범위의 pH에서 생육이 가능하고 저영양원에서도 생육이 가능한 Bacillus속 및 Erwinia속의 길항 미생물에 관한 것이다.The present invention relates to a method for biological control of softening of ginseng. More specifically, the present invention is to provide an antagonistic bacterium useful for the control of various ginseng diseases caused by soil bacteria according to the operation of ginseng. More specifically, the present invention is an antagonist of the genus Bacillus and Erwinia, which has a high antagonistic ability against various pathogens, has high affinity for ginseng, is capable of growing at a wide range of pH, and can be grown at low nutritional levels. It is about.

다년생의 반음지성 숙근초로 오가과에 속하는 인삼(Panax ginseng)은 생육적온이 20℃ 전후이고 30℃이상에서는 생육장해를 받기 때문에 주로 북반구의 극동지방에 자생하거나 재배되며 우리나라에서는 주로 경기도 북부지역 및 풍기와 금산지방에서 재배된다. 인삼은 한 장소에서 4~6년 동안 재배되므로 연작장해의 발생 가능성이 매우 높기 때문에 농가에서는 초작지만을 찾아 재배하려고 한다. 이로인해 우리나라의 인삼 재배면적은 1990년 3,664ha, 1993년 2,914ha, 1996년에 2,265ha로서 매년 감소추세에 있다. 연작장해는 Cylindrocarpon destructans, Fusarium solani, Phytophthora cactorum, Erwinia carotovora, Pseudomonas fluorescens 등의 병원균들이 단독으로 또는 복합적으로 작용하기 때문에 발생한다. 또한 연작지가 아니더라도 모잘록병을 일으키는 Rhozoctonia solani, 뿌리썩음병의 Botrytis cinerea, 점무늬병의 Alternaria panax 등에 의한 피해가 막대하다. 이러한 상기 병해들에 대한 종래의 방제법으로는 첫째, 토양내 병원균의 밀도를 감소시키거나 발병을 억제시키기 위한 유기물 시용 등을 통한 예정지 관리 및 토양훈증제를 이용한 화학적 방제와 둘째, 답전 윤환의 논삼 재배법 등에 의한 경종적 방제가 가장 널리 사용되고 있다. 그러나 상기의 방법들은 토양전염성 병원균들에 대한 방제가 거의 불가능하며, 또한 토양훈증제의 사용은 토양 생태계의 파괴, 농약 잔류에 의한 식품 안정성의 위협과 환경오염 등의 문제를 발생시킨다. 특히 인삼의 토양병들은 다양한 온도 범위에서 감염이 성립되므로 한번 감염되면 비록 병의 진전은 느리지만 연중 피해를 받게 된다. 그러므로, 효과적인 토양병의 방제를 위해서는 인삼재배 특성상 병방제 효과가 지속적이고 환경에 안정적인 생물학적 방제가 가장 적합함을 알 수 있다.Panax ginseng belonging to Oga, a perennial semi-negative herb, is grown or grown in the Far East of the Northern Hemisphere because of its growth temperature around 20 ° C and above 30 ° C. It is grown in Geumsan province. Since ginseng is grown for four to six years in one place, there is a high chance of serial disturbances, so farmers try to find only crops. As a result, the ginseng cultivation area in Korea is 3,664 ha in 1990, 2,914 ha in 1993, and 2,265 ha in 1996. Serial disturbance occurs because pathogens such as Cylindrocarpon destructans, Fusarium solani, Phytophthora cactorum, Erwinia carotovora, and Pseudomonas fluorescens act alone or in combination. In addition, even if it is not arable land, the damage caused by Rhozoctonia solani, which causes mozzarella disease, Botrytis cinerea of root rot disease, and Alternaria panax of spot pattern disease, is enormous. Conventional control methods for the above-mentioned diseases include, first, the management of the planned site through the application of organic matter to reduce the density of the pathogens in the soil or to suppress the onset, the chemical control using the soil fumigant, and the second, the field of cultivation of paddy ginseng, etc. Seedling control is most widely used. However, the above methods are almost impossible to control the soil infectious pathogens, and the use of soil fumigants causes problems such as destruction of soil ecosystem, threat of food stability due to pesticide residue and environmental pollution. In particular, soil diseases of ginseng are infected at various temperature ranges, so once infected, the disease progresses year-round, although the disease progresses slowly. Therefore, it can be seen that for effective soil disease control, biological control, which is stable and environmentally stable, is most suitable for ginseng cultivation characteristics.

따라서, 본 발명의 목적은 생물학적 방제를 위하여 여러 병원균들에 대해 종합적으로 높은 길항력을 갖고 인삼에 대한 높은 친화성을 가지며 넓은 범위의 pH에서 생육이 가능하고 저영양원에서도 생육이 가능한 Bacillus속의 16P5 균주를 제공함에 있다.Therefore, an object of the present invention is a 16P5 strain of the genus Bacillus, which has a high antagonism against various pathogens, has a high affinity for ginseng, can grow at a wide range of pH, and can grow at low nutrients for biological control. In providing.

상기의 목적은 주요 병원균과 인삼의 재배특성을 고려하여 주요 인삼병에 대한 유용길항미생물을 특정조건에 의해 선발하고, 선발된 균들의 특성을 조사하여 여러 병원균들에 대해 종합적으로 높은 길항력을 갖고 인삼에 대한 높은 친화성을 가지며 넓은 범위의 pH에서 생육이 가능하고 저영양원에서도 생육이 가능한 Bacillus속의 16P5 균주를 제공하므로써 달성한다.The purpose of the above is to select useful antagonistic microorganisms for major ginseng diseases under specific conditions in consideration of the cultivation characteristics of the main pathogens and ginseng, and to investigate the characteristics of the selected microorganisms, This is achieved by providing 16P5 strains of Bacillus genus which have high affinity for ginseng and are capable of growing at a wide range of pH and can be grown at low nutrients.

이하, 본 발명의 구성 및 작용을 설명한다.Hereinafter, the configuration and operation of the present invention.

도1은 F. solani, B. cinerea, C. destructans, R. solani, Phytophthora cactorum, Pythium sp. Alternaria panax에 대한 길항작용을 갖는 박테리아의 저지부분을 표시한 것이다.1 shows F. solani, B. cinerea, C. destructans, R. solani, Phytophthora cactorum, Pythium sp. It indicates the blocking part of bacteria having antagonism against Alternaria panax.

도2는 pH에 따른 항박테리아의 영향을 표시한 것으로, A는 다양한 PDA 농도에서 아가 플레이트상의 Fusarium solani에 대한 항균활성을 갖는 S21의 항균활성도를 표시한 것이고, B는 King's B agar plate(KBA)상에 희석된 S21의 박테리아 현탁액의 항균활성을 표시한 것이다.Figure 2 shows the effect of antibacterial according to pH, A shows the antimicrobial activity of S21 having antimicrobial activity against Fusarium solani on agar plate at various PDA concentrations, B is King's B agar plate (KBA) The antimicrobial activity of the bacterial suspension of S21 diluted in phase is shown.

본 발명은 주요 병원균과 인삼의 재배특성을 고려하여 주요 인삼병에 대한 유용 길항미생물을 선발하는 단계; 선발된 균들의 생육적온등의 특성을 조사하는 단계; 및 선발된 균들의 특성을 통해 길항세균의 동정 및 특성을 조사하는 단계로 이루어짐을 특징으로 한다. 이하, 본 발명의 구체적인 구성 및 작용을 하기 실시예들을 통하여 상세히 설명하지만 본 발명의 범위가 하기 실시예에만 한정되는 것은 아니다.The present invention comprises the steps of selecting useful antagonists for major ginseng diseases in consideration of the cultivation characteristics of the main pathogens and ginseng; Examining characteristics such as growth temperature of the selected bacteria; And through the characteristics of the selected bacteria characterized in that the step consisting of identifying and characterizing the antagonistic bacteria. Hereinafter, the specific configuration and operation of the present invention will be described in detail with reference to the following examples, but the scope of the present invention is not limited only to the following examples.

실시예 1. 병원균 분리Example 1 Pathogen Isolation

충남 논산, 충북 음성, 경기 평택, 강원 횡성지역의 인삼 재배포장에서 1년생부터 5년생을 대상으로 이병증상을 보이는 잎, 줄기, 뿌리를 0.5㎝ 크기로 절단하여 0.5% 소디움 하이포아염소산염 용액에서 3분간 침지하였다. 그후 멸균수로 세척하고 습기를 제거한 후 워터 아가상에 치상하여 20℃ 암상태로 배양 후 형성된 단포자 또는 균사를 순수 분리하였다. 그리고 Potato dextrose agar(PDA), V8 juice agar(V8A), Spezieller nahrstoffarmer yeast extract agar(SNA) 등의 배지에서 각각 배양하여 포자의 형성유무, 포자 및 균사의 형태 등에 따라 동정하였고 동정된 균들은 단포자 또는 단균사로 접종, 배양한 후 5℃에 보관하면서 실험에 사용하였다. 실험결과, 이병인삼에서 F. solani, B. cinerea, C. destructans, R. solani AG-4, P.cactorum, Pythium sp., A panax 등 7종의 균이 분리되었다.Cut the leaves, stems, and roots showing the symptoms of this disease from 0.5% sodium hypochlorite solution in the ginseng cultivation packages in Nonsan, Chungbuk Eum, Gyeonggi Pyeongtaek, and Gangwon Hoengseong, Chungnam, Korea. Immerse for minutes. After washing with sterile water, the moisture was removed and then placed on water agar and incubated in 20 ℃ dark state to form a pure spore or mycelium. Potato dextrose agar (PDA), V8 juice agar (V8A), and Spezieller nahrstoffarmer yeast extract agar (SNA) were incubated in medium, and identified according to the formation of spores, spores, and mycelia. Or inoculated and cultured with monomycelia, it was used for experiments while storing at 5 ℃. As a result, seven species of B. ginseng were isolated from F. solani, B. cinerea, C. destructans, R. solani AG-4, P.cactorum, Pythium sp., And A panax.

실시예 2. 병원성 조사Example 2. Pathogenicity Investigation

이병인삼에서 분리된 균들중 주요 토양병원균인 C. destructans, F. solani, R. solani의 병원성 유무를 조사하기 위하여 멸균된 밀기울에서 28일 동안 각각 배양하여 접종원으로 사용하였다. 상토 100g에 접종원을 생중량 5g씩 넣어 잘 혼합한 후 직경 9㎝의 비닐 포트에 넣고, 3~5mm 발아된 인삼종자를 5립씩 4반복으로 파종하였다. 처리된 각 포트는 30일간 Growth Chamber에서 12시간은 15℃로, 12시간은 20℃의 광조건으로 인삼 재배조건에 적합하도록 유지하면서 재배하였다. 파종후 14일째 및 30일째에 발아된 모잘록증상과 시들음 증상을 보인 발아유묘수를 조사하였다. 실험결과, 하기 표1에서 볼 수 있듯이 7일 후의 발아율은 F. solani와 C. destructans 경우 93%로 같았으나 R. solani의 경우는 73%로 다소 낮은 발아율을 보였다. 또한 F. solani와 C. destructans의 처리구에서는 파종후 14일째 대조구와 비교하여 외관상 큰 차이가 없었으나 30일째에는 F. solani와 C. destructans의 처리구에서 시들음 증상이 나타났다. 반면 R. solani 처리구는 14일째 91%의 높은 입고율을 보였고 30일째에는 모두 고사하는 뚜렷한 병징을 보였다.In order to investigate the pathogenicity of C. destructans, F. solani, and R. solani, the major soil pathogens isolated from two ginsengs were incubated in sterilized bran for 28 days and used as inoculum. 100 g of inoculum was added to the inoculum 5g by weight, mixed well, and placed in a vinyl pot of 9 cm in diameter, and 3 to 5 mm germinated ginseng seeds were sown in four repeats of 5 grains each. Each pot was grown in a growth chamber for 30 days at 15 ° C. for 12 hours and at 12 ° C. under 20 ° C. under the light conditions of ginseng. Germination seedlings with germinated mozacosis and wilting symptoms were examined at 14 and 30 days after sowing. As a result, as shown in Table 1, the germination rate after 7 days was the same as 93% for F. solani and C. destructans, but slightly lower to 73% for R. solani. In addition, the treatments of F. solani and C. destructans showed no significant difference compared to the control on day 14 after sowing. However, on day 30, treatment with F. solani and C. destructans showed wilting symptoms. On the other hand, R. solani treated group showed a high receipt rate of 91% at 14 days, and all of them showed a clear symptom.

개갑후 발아한 인삼종자에서 채취한 Cylindrocarpon destructans, Rhizoctonia silani, Fusarium solani의 병원성 조사Pathogenicity of Cylindrocarpon destructans, Rhizoctonia silani, and Fusarium solani from Germinated Ginseng Seed after Opening PathogenicityPathogenicity Pathogenb Pathogen b controlcontrol C. destructansC. destructans R. solaniR. solani F. solaniF. solani Emergencing rateEmergencing rate 9393 7373 9393 8787 Standing ratecafter 14daysStanding rate c after 14days 9393 99 9393 8787 Standing rate after 30daysStanding rate after 30days 2424 00 8080 8787

실시예 3. 인삼병원균들의 생육적온 조사Example 3 Growth Temperature of Ginseng Pathogens

상기 실시예 1에서 분리한 병원균을 Potato dextrose agar(PDA), V8 juice agar(V8A), Spezieller nahrstoffarmer yeast extract agar(SNA)에서 배양한 후 Cork borer로 떼어 각각의 배지중앙에 접종하여 암상태하에서 배양하여 24시간 간격으로 자란 균사의 길이를 측정하였고 측정한 기간은 병원균이 9㎝ Petri dish상에 전부 생육할 때까지 3번 반복하여 측정하여 이들의 평균값을 계산하였다. 실험결과, B. cinerea와 C. destructans는 15~20℃에서 균사의 생장이 좋았으며 R. solani, P.cactorum, Phythium sp., A panax는 15~30℃의 넓은 온도범위에서 균사의 생장이 좋았다. F. solani의 경우는 20~30℃에서 균사의 생장이 좋았다. 5~10℃에서는 B. cinerea와 C. destructans의 균사생장이 다른균에 비해 좋았다.The pathogens isolated in Example 1 were incubated in Potato dextrose agar (PDA), V8 juice agar (V8A), Spezieller nahrstoffarmer yeast extract agar (SNA), separated with Cork borer, inoculated in the center of each medium, and cultured under cancer. The length of the mycelia grown at intervals of 24 hours was measured, and the measured period was repeated three times until all pathogens were grown on a 9cm Petri dish to calculate their average value. As a result, B. cinerea and C. destructans showed good mycelial growth at 15 ~ 20 ℃, and R. solani, P.cactorum, Phythium sp., And A panax showed mycelial growth at wide temperature range of 15 ~ 30 ℃. it was good. In case of F. solani, mycelial growth was good at 20 ~ 30 ℃. Mycelial growth of B. cinerea and C. destructans was better than other bacteria at 5 ~ 10 ℃.

실시예 4. 길항세균의 분리와 선발 및 항균활성 측정Example 4. Isolation, Selection and Antimicrobial Activity of Antagonists

길항세균의 분리는 Burr등의 방법을 이용하였다. 즉, 인삼 재배년수별로 건전인삼의 근권 10~15㎝ 깊이의 흙을 한 포장당 3점씩 채취하여 채로 걸러 실험에 사용하였다. 채집된 토양을 King's B agar(KBA) 배지에 희석평판법에 따라 형성되는 세균 colony 수를 40~80 colony forming unit(CFU)/petri dish로 조정한 후 25℃에서 배양하였다. 24시간 배양 후 인삼병원균인 4종의 병원진균 F. solani, A. panax, B. cinerea, C. destructans의 포자현탁액(108spore/㎖)을 만들어 각각의 KBA표면에 분무 접종하였다. 분무후 24시간동안 다시 배양하여 병원균의 포자발아 및 생육을 저지하는 세균 콜로니를 조사하였다. 항균활성을 나타내는 세균 콜로니는 순수분리하여 동결건조한 후 -65℃에 보관하였다. 1차 스크리닝을 통해 선발된 길항세균들을 F. solani, B. cinerea, C. destructans, R. solani, Phytophthora cactorum, Pythium sp. 등 인삼 병원균들과 배지상에서 대치배양하여 항균활성을 크게 나타내는 것을 2차 선발하였다. 2차 선발된 길항세균은 10℃, 15℃, 20℃, 25℃의 온도조건하에 PDA배지상에서 각각의 병원균들과 대치배양하여 저지원의 크기를 측정하고 각 온도별 항균활성을 조사하였다. 실시결과, 도1에서 보듯이 F. solani, B. cinerea, C. destructans, R. solani, Phytophthora cactorum, Pythium sp. Alternaria panax 등 7종의 병원진균에 고루 항균활성을 나타내는 길항세균은 B2, B12, S21, 16P5, 17N6-1 균주가 선발되었다. 특히, 이들중 S21과 16P5 균주는 5종의 토양 병원균에 대해 저지원의 크기가 각각 최대 13mm와 10mm에 이를 정도로 항균효과가 높았다. 또한 이들은 하기 표2에서 볼수 있듯이 10℃ 이하의 낮은 온도에서도 C. destructans, F. Solani에 대해서는 보다 높은 온도조건에서와 같은 길항효과를 나타낸 반면 Pythium sp.에는 길항효과가 적었다.Isolation of antagonistic bacteria was performed using Burr et al. In other words, three points per root of healthy ginseng were harvested three times per package and used for the experiment. Collected soil was adjusted to 40 ~ 80 colony forming unit (CFU) / petri dish of bacterial colony formed by dilution plate method in King's B agar (KBA) medium and incubated at 25 ℃. After incubation for 24 hours, spore suspensions (10 8 spores / ml) of four pathogens F. solani, A. panax, B. cinerea and C. destructans, which were ginseng pathogens, were prepared and sprayed on the surface of each KBA. Incubate for 24 hours after spraying to investigate bacterial colonies that inhibit spore germination and growth of pathogens. Bacterial colonies exhibiting antimicrobial activity were purely separated, lyophilized and stored at -65 ° C. Antagonists selected through primary screening were identified as F. solani, B. cinerea, C. destructans, R. solani, Phytophthora cactorum, Pythium sp. Secondary selection was carried out in the culture medium and the ginseng pathogens showing a large antibacterial activity. Secondarily selected antagonistic bacteria were incubated with each pathogen on PDA medium under the temperature condition of 10 ℃, 15 ℃, 20 ℃, 25 ℃ to measure the size of low support and antibacterial activity at each temperature. As a result, as shown in Figure 1 F. solani, B. cinerea, C. destructans, R. solani, Phytophthora cactorum, Pythium sp. Antagonistic bacterium that showed antibacterial activity against 7 pathogens including Alternaria panax was selected as B2, B12, S21, 16P5, 17N6-1 strains. In particular, the S21 and 16P5 strains had high antimicrobial effects, reaching a maximum of 13 mm and 10 mm, respectively, for the five soil pathogens. In addition, as shown in Table 2 below, they exhibited the same antagonistic effect as C. destructans and F. Solani at lower temperatures of 10 ° C. or lower, whereas Pythium sp.

실시예 5. 길항세균의 동정 및 배양적 특성조사Example 5 Identification and Culture Characterization of Antagonistic Bacteria

상기 실시예 4에서 선발된 길항세균들은 'Bergey's manual of determinative bacteriology ninth edition'을 이용하여 동정하였다. 즉, pH가 4.5, 5.0, 6.0, 7.0, 8.0인 King's B 액체배지 200㎖에 길항세균의 밀도를 조사하였으며, 균의 영양상태별 항균활성은 Potato dextrose broth(Difco)를 증류수 1L당 2.4g(10%), 7.2g(30%), 12.0g(50%), 24.0g(100%)씩 첨가한 후 한천을 20g씩 넣어 배지를 조제한 후 선발된 길항세균들과 F. solani를 25℃에서 14일간 대치 배양하여 저지원의 크기를 측정하였다. 실험결과, 하기 표3에서 보듯이 16P5, 17N6-1, B12는 Bacillus속 이였으며, B2는 Pseudomonas속, S21균주는 Erwinia속 세균으로 동정되었다. 상기의 길항세균중 가장 유용한 길항력을 갖는 Bacillus속의 16P5 균주를 1998년 4월 27일 한국과학기술연구원 생명공학연구소내 유전자은행에 기탁번호 KCTC 8887P로 기탁하였다. 또한, 배양특성은 도2A에서 보듯이 S21 균주의 pH별 생육 범위는 pH 4.0~8.0으로 인삼재배토양의 pH 5.0~6.0과 잘 부합되었다. 그리고, 영양과 항균활성과의 관계를 알아보기 위해 대치배양해본 결과 도2B에서 보듯이 PDA의 농도를 10% 희석시킨 배지에서도 항균활성을 보였다.Antagonists selected in Example 4 were identified using 'Bergey's manual of determinative bacteriology ninth edition'. In other words, the density of antagonistic bacteria was investigated in 200 ml of King's B liquid medium with pH of 4.5, 5.0, 6.0, 7.0, and 8.0, and the antimicrobial activity of each bacterium was 2.4g (1g) of Potato dextrose broth (Difco) 10%), 7.2g (30%), 12.0g (50%), 24.0g (100%), and then added agar 20g each to prepare a medium, and then select antagonists and F. solani at 25 ℃. The size of the low support was measured by replacement culture for 14 days. As a result, as shown in Table 3, 16P5, 17N6-1 and B12 were Bacillus genus, B2 was identified as Pseudomonas genus and S21 strain Erwinia genus. The 16P5 strain of the genus Bacillus, which has the most useful antagonistic power among the antagonistic bacteria, was deposited on April 27, 1998 to the gene bank of the Biotechnology Research Institute of the Korea Institute of Science and Technology as deposit number KCTC 8887P. In addition, the culture characteristics of the S21 strain as shown in Figure 2A growth range of pH 4.0 ~ 8.0 was well matched with pH 5.0 ~ 6.0 of ginseng cultivated soil. And, in order to examine the relationship between nutrition and antimicrobial activity, as a result of replacement culture, as shown in FIG.

선택된 길항세균의 일반적 특성General Characteristics of Selected Antagonists CharacterCharacter Antogonistic bacteriaAntogonistic bacteria S21S21 B2B2 16P516P5 17N6-117N6-1 B12B12 Gram reactionGram reaction -- -- ++ ++ ++ Motility in liquid mediaMotility in liquid media ++ ++ ++ ++ ++ Yellow colonies on NA, YDC or NBYYellow colonies on NA, YDC or NBY ++ -- -- -- -- Fluorescent pigmentFluorescent pigment -- ++ -- -- -- Gelatin liquefactionGelatin liquefaction ++ -- ++ -- ++ Grow anaerobicallyGrow anaerobically ++ ++ -- -- ++ Rods, straight or curvedRods, straight or curved OvoidOvoid -- -- -- -- Grow at 60℃ or higherGrow at 60 ℃ or higher -- -- ++ ++ ++ Rod-shaped in young cultureRod-shaped in young culture -- ++ ++ ++ ++ Endospore producedEndospore produced -- -- ++ ++ ++

본 발명은 상세히 설명한 바와 같이 인삼 연작장해의 생물학적 방제방법을 제공하는 효과가 있으며 또 인삼 연작장해를 방제할 수 있고 유용한 길항세균을 제공한 효과가 있을 뿐만 아니라, 특히 Bacillus속의 16P5 균주의 선발은 인삼 연작장해를 방제할 수 있고 여러 병원균들에 대해 종합적으로 높은 길항력을 가지며 인삼에 대한 높은 친화성을 가지고 넓은범위의 pH에서 생육이 가능하며 저영양원에서도 생육이 가능한 효과를 가지므로 생물농약산업상 매우 유용한 발명이다.The present invention has the effect of providing a biological control method of ginseng rot as described in detail and can control the ginseng rot and provide a useful antagonistic bacteria, in particular the selection of 16P5 strain of Bacillus genus ginseng It can control crop disturbance, has high antagonistic ability against various pathogens, has high affinity for ginseng, can grow in a wide range of pH, and can grow in low nutritional sources. It is a very useful invention.

Claims (2)

인삼의 토양전염성 병원균들에 대한 길항세균으로 토양에서 분리한 Bacillus sp. 16P5(KCTC 8887P) 균주.Bacillus sp. Isolated from soil as antagonistic bacteria against soil infectious pathogens of ginseng. 16P5 (KCTC 8887P) strain. 길항세균 Bacillus sp. 16P5(KCTC 8887P) 균주를 사용함을 특징으로 하는 인삼 연작장해의 생물학적 방제방법.Antagonist Bacillus sp. Biological control method of ginseng softening disorders characterized by using 16P5 (KCTC 8887P) strain.
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