JPS6350735A - Method for sampling liquid specimen from capillary liquid specimen container - Google Patents
Method for sampling liquid specimen from capillary liquid specimen containerInfo
- Publication number
- JPS6350735A JPS6350735A JP19581786A JP19581786A JPS6350735A JP S6350735 A JPS6350735 A JP S6350735A JP 19581786 A JP19581786 A JP 19581786A JP 19581786 A JP19581786 A JP 19581786A JP S6350735 A JPS6350735 A JP S6350735A
- Authority
- JP
- Japan
- Prior art keywords
- liquid sample
- sample
- suction
- suction nozzle
- capillary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 104
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000005070 sampling Methods 0.000 title claims abstract 6
- 238000001514 detection method Methods 0.000 abstract description 15
- 239000000523 sample Substances 0.000 description 142
- 238000004458 analytical method Methods 0.000 description 13
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 239000002966 varnish Substances 0.000 description 5
- 239000012530 fluid Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- -1 for example Substances 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000001819 pancreatic juice Anatomy 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
【発明の詳細な説明】
（イ）産業上の利用分野
本発明は、キャピラリ液体試料容器内からの液体試料採
取用吸引ノズルによる液体試料のｒＡ取方法に関し、特
に、液体クロマトグラフィ分析法、７０−インジェクシ
ョン分析法、その他微量液体分析法における、キャピラ
リ液体試料容器からの液体試料採取用吸引ノズルによる
液体試料の採取方法に関する。DETAILED DESCRIPTION OF THE INVENTION (a) Industrial Application Field The present invention relates to a method for collecting a liquid sample using a suction nozzle for collecting a liquid sample from a capillary liquid sample container, and in particular to a liquid chromatography analysis method, 70- The present invention relates to a method for collecting a liquid sample using a suction nozzle for collecting a liquid sample from a capillary liquid sample container in injection analysis and other trace liquid analysis methods.
また、本発明は、血液、血漿、血清、リンパ液等の体液
、尿等の***物、胃液、膵液、胆汁、唾液、汗等の分泌
物、腹水、力水、関節腔液等の穿刺液等の液体試料につ
いての自動化学分析におけるキャピラリ液体試料容器か
らの液体試料分注７ズルによる液体試料の吸引採取方法
に関する。The present invention also covers body fluids such as blood, plasma, serum, and lymph, excreta such as urine, secretions such as gastric juice, pancreatic juice, bile, saliva, and sweat, ascites, hydrodynamic fluid, and puncture fluid such as joint cavity fluid. The present invention relates to a method for suctioning and collecting a liquid sample by dispensing a liquid sample from a capillary liquid sample container using seven screws in automatic chemical analysis of a liquid sample.
（ロ）従来の技術
体液、生体試料の中で、例えば血液は、全身を循環して
組織１こ酸素、栄１！物質、ホルモンなどの物質を与え
、組織から老廃物を運び去る働きをしているので、その
組成は、分（（の＃３胞や１■織の代謝が総合的に反映
しており、身体の何処かに病変があると、それに応じて
変化する。したがって、血液等の成分分析値は、各種病
気の診断病勢や治療効果の１′−ｑ定、治療指針等に利
用されている。(b) Conventional technology Among body fluids and biological samples, for example, blood circulates throughout the body, absorbing oxygen and oxygen in the tissues. It functions to provide substances such as substances and hormones and to carry away waste products from tissues, so its composition comprehensively reflects the metabolism of #3 cells and #1 tissue of the body. If there is a lesion anywhere in the body, the blood will change accordingly.Therefore, component analysis values of blood, etc. are used for diagnosis of various diseases, 1'-q determination of therapeutic effects, treatment guidelines, etc.
しかも、このような医療情報は、単一分析項目によるよ
りも、複数の分析項目を組み合わせた方が診断確度が高
くなるので、一検体についての必要な分析項目が増えて
おり、それに伴い高い分析精度が要求されるようになっ
ている。Moreover, for such medical information, the diagnostic accuracy is higher when multiple analysis items are combined than from a single analysis item, so the number of analysis items required for one sample is increasing, and accordingly, high-quality analysis is required. Precision is now required.
しかし、このような診断、治療指針等に利用される分析
の多くは、患者を対象としており、その採血量は思考の
病状によって制限を受け、殊に、乳幼児１こあっては、
全体量も少いためにその採血量は着しく制限される。However, most of the analyzes used for diagnosis and treatment guidelines target patients, and the amount of blood collected is limited by the patient's medical condition, especially for infants and young children.
Since the total volume is small, the amount of blood collected is severely limited.
このような微量の液体試料は、所謂キャピラリ液体試料
容器に収容される。キャピラリ液体試料容器は、採取さ
れた検体量に応じて、その容量は適宜選択され、寸法に
変化はあるが、例えば、外径的１．５ｕ、内径的０．８
５ｗ、ｆｆｌさ約７５ｆｌという極めて小形のものであ
り、これに収容された液体試料は、例えば、外径的０．
７２ｆｌ、内径的０．５ｔＲの試料採取用吸引ノズルを
用いて吸引採取されて、例えば、高速液体クロマトグラ
フィ分析装置の試料導入部又は７０一インジエクシａン
分析装置の試料導入部に導管を経由して送られ、或は、
自動分析装置の試料分注位置で反応管に吐出される。Such a small amount of liquid sample is stored in a so-called capillary liquid sample container. The capacity of the capillary liquid sample container is appropriately selected depending on the amount of sample collected, and the dimensions may vary, but for example, the outer diameter is 1.5 μ, the inner diameter is 0.8 μ.
It is extremely small, with an outer diameter of 0.5w and an ffl size of approximately 75fl.
The sample is collected by suction using a sample collection suction nozzle of 72 fl and 0.5 tR in internal diameter, and is then transferred via a conduit to the sample introduction section of a high-performance liquid chromatography analyzer or the sample introduction section of a 70-indicator analyzer, for example. sent or
It is discharged into the reaction tube at the sample dispensing position of the automatic analyzer.
（ハ）発明が解決しようとする問題点
このように、キャピラリ液体試料容器から液体試料を試
料採取用吸引ノズルを用いて吸引採取する場合、空気を
取り込まないようにするために、該キャピラリ容器内の
液体試料中法（に吸引ノズルを入れて採取している。こ
のようにすると、液体試料中に浸漬した吸引ノズルの容
積分だけ、該キャピラリ容器内の液体試料の嵩が増し、
該キャピラリ容器内の液体試料が該キャピラリ容器上端
から溢れ出て、キャピラリホルダを汚染することとなる
。そこで、この汚染による分析誤差を避けるために、試
料採取毎に、キャピラリホルダを洗浄することが必要と
なり、特別に洗浄工程及び乾燥工程を要し、問題である
。また、乾燥が充分でないと、次の試料採取時における
液体試料への吸引ノズルの浸漬により、液体試料の溢流
部分が希釈されて分析誤差を生ずるなど問題である。(c) Problems to be Solved by the Invention As described above, when a liquid sample is collected by suction from a capillary liquid sample container using a sample collection suction nozzle, in order to prevent air from being taken in, it is necessary to A suction nozzle is inserted into the liquid sample to collect the sample. In this way, the volume of the liquid sample in the capillary container increases by the volume of the suction nozzle immersed in the liquid sample.
The liquid sample in the capillary container will overflow from the top of the capillary container and contaminate the capillary holder. Therefore, in order to avoid analysis errors due to this contamination, it is necessary to clean the capillary holder every time a sample is collected, which requires a special cleaning process and drying process, which is a problem. In addition, if the drying is not sufficient, the overflow portion of the liquid sample will be diluted due to immersion of the suction nozzle into the liquid sample when the next sample is collected, resulting in problems such as analysis errors.
また、キャピラリ液体試料容器内への吸引ノズルの挿入
が偏心して行われると、吸引ノズル壁が該キャピラリ容
器壁に近い側では、吸引による液位の低下が他に比較し
て急速に進むために、空気を吸引し勝であり問題である
。そこで、吸引ノズルのキャピラリ容器への挿入には、
キャピラリ容器に対して、吸引ノズルが偏心しないよう
に中心合せを要することになり、その精密さが改めて要
求されることになって、依然問題が残ることになる。Additionally, if the suction nozzle is eccentrically inserted into the capillary liquid sample container, the liquid level will drop more rapidly due to suction on the side where the suction nozzle wall is closer to the capillary container wall than on the other side. , suction of air is a problem. Therefore, when inserting the suction nozzle into the capillary container,
It is necessary to center the suction nozzle with respect to the capillary container so that it does not become eccentric, and this requires new precision, and the problem still remains.
本発明は、以上のような、従来のキャピラリ液体試料容
器から液体試料を試料採取用吸引ノズルを用いて吸引採
取する方法における問題点を解決することを目的として
いる。An object of the present invention is to solve the problems described above in the conventional method of sucking and collecting a liquid sample from a capillary liquid sample container using a sample collection suction nozzle.
（ニ）問題点を解決するための手段
本発明は、キでピラリ液体試料容器から液体試料を試料
採取用吸引ノズルを用いて吸引採取する場合に、液体試
料がキャピラリ液体試料容器がら溢流することがなく、
キャピラリ液体試料容器への試料採取用吸引ノズルの挿
入に、中心合せ等の精密さが要求されない液体試料の採
取方法を提供するものである。(d) Means for Solving the Problems The present invention provides a solution to the problem that when a liquid sample is collected by suction from a capillary liquid sample container using a suction nozzle for sample collection, the liquid sample overflows from the capillary liquid sample container. Without a doubt,
The present invention provides a liquid sample collection method that does not require precision such as centering when inserting a sample collection suction nozzle into a capillary liquid sample container.
すなわち、本発明は、キャピラリ液体試料容器内に液体
試料採取用吸引ノズルを挿入して、キャピラリ液体試料
容器内に収容されている液体試料を該吸引ノズル内に吸
込み液体試料の採取を行うキャピラリ液体試料容器がら
の液体試料採取方法において、少くとも液体試料採取用
１及引ノズル開口部をキャピラリ液体試料容器内の液体
試料内に浸漬させ、続いて、該吸引ノズル開口部を該液
体試料内を下方に移動させながら、該吸引ノズル内への
液体試料の吸引を開始することを特徴とするキャピラリ
液体試料容器からの液体試料採取方法にある。That is, the present invention provides a capillary liquid sample collection system in which a suction nozzle for liquid sample collection is inserted into a capillary liquid sample container, and a liquid sample contained in the capillary liquid sample container is sucked into the suction nozzle to collect the liquid sample. In a method for collecting a liquid sample from a sample container, at least one suction nozzle opening for liquid sample collection is immersed into a liquid sample in a capillary liquid sample container, and then the suction nozzle opening is immersed into the liquid sample. A method for collecting a liquid sample from a capillary liquid sample container, characterized by starting suction of the liquid sample into the suction nozzle while moving the liquid sample downward.
本発明においても、従来法と同様に、キャピラリ液体試
料容器内に液体試料採取用吸引ノズルをトｐ人するに、
該吸引ノズル開口部を、該キャピラリ容器内の液体試料
内に浸漬させるが、本発明においては、液体試料の嵩が
キャピラリ液体試料容器の上端を越える前に液体試料の
吸引が開始される。そこで、液体試料の嵩は、キャピラ
リ液体試料容器の上端を越えることなく低下し始めるの
で、この液体試料の嵩の低下に伴い、液体試料採取用吸
引ノズル開口部を下降させながら、液体試料の所定量を
該吸引ノズル内に吸引し、液体試料を採取する。In the present invention, as in the conventional method, a suction nozzle for collecting a liquid sample is placed inside a capillary liquid sample container.
The suction nozzle opening is immersed in the liquid sample in the capillary container, and in the present invention, suction of the liquid sample is started before the volume of the liquid sample exceeds the upper end of the capillary liquid sample container. Therefore, the volume of the liquid sample starts to decrease before it exceeds the upper end of the capillary liquid sample container, so as the volume of the liquid sample decreases, the liquid sample is A fixed amount is drawn into the suction nozzle and a liquid sample is taken.
本発明において、液体試料採取用吸引ノズルの下降は、
周囲から空気の吸入を避けるように行われる。前記吸引
ノズルの下降を行うステッピングモータ等の動力源と、
該吸引ノズルの吸引を行う、例乏ばピペッタのプランジ
ャの作動を行うステッピングモータ等の動力源を同期さ
せて行われる。In the present invention, the lowering of the suction nozzle for liquid sample collection is
This is done to avoid inhaling air from the surrounding area. a power source such as a stepping motor that lowers the suction nozzle;
This is carried out by synchronizing a power source such as a stepping motor that performs suction by the suction nozzle, for example, a stepping motor that operates a plunger of a pipettor.
この同期は、液体試料採取用吸引ノズル所定の位置まで
下降したところで、該吸引ノズルのピペッタのプランジ
ャの吸引動作が始まるように打われる。前記吸引ノズル
の位置の検出は、検出精度の高い各種位置検出方式によ
って行われる。このような位置検出方式としては、カム
、リンク、歯車装置等の機械式の位置検出ｌｆ！索、マ
イクロスイッチ、近接スイッチ、電気マイクロメータ、
電気式のリニアスケールパルスモータ等の電気式の位置
検出機業、空気マイクロメータ等の流体式位置検出機素
及び光電スイッチ、充電式リニアスケール等の光学式位
置検出ＶＩｉ索などを使用することができる。This synchronization is performed so that when the suction nozzle for collecting a liquid sample descends to a predetermined position, the plunger of the pipetter of the suction nozzle starts the suction operation. The position of the suction nozzle is detected by various position detection methods with high detection accuracy. Such position detection methods include mechanical position detection lf! such as cams, links, gear devices, etc. cable, microswitch, proximity switch, electric micrometer,
Electric position detection devices such as electric linear scale pulse motors, fluid position detection elements such as air micrometers, photoelectric switches, and optical position detection VIi cables such as rechargeable linear scales can be used. can.
（ホ）作用
本発明は、液体試料採取用吸引ノズル閉口部をキャピラ
リ液体試料容器内の液体試料内に浸漬させ、続いて、該
吸引ノズル開口部を下方に移動させながら、液体試料の
吸引を行うので、空気を吸入することな（、また、該吸
引ノズルを浸漬するのも拘らず、液体試料の嵩が、該キ
ャピラリ試料容器の上縁を越えることな（、液体試料の
吸引による採取を行うことができる。(E) Function The present invention involves immersing the closing part of a suction nozzle for liquid sample collection into a liquid sample in a capillary liquid sample container, and then suctioning the liquid sample while moving the suction nozzle opening downward. Therefore, the volume of the liquid sample should not exceed the upper edge of the capillary sample container (despite the suction nozzle being immersed). It can be carried out.
また、本発明においては、液体試料採取用吸引ノズルが
キャピラリ液体試料容器内をスムーズに下降できれば、
多少中心合せが精密でなくても、該吸引ノズルの下降段
階で、該吸引ノズルの周囲の液位の不均衡はＭ消できる
ので、液位の不均衡による空気の吸入を避けて、液体試
料の吸引採取を行うことができる。In addition, in the present invention, if the suction nozzle for liquid sample collection can descend smoothly inside the capillary liquid sample container,
Even if the center alignment is not precise, the imbalance in the liquid level around the suction nozzle can be eliminated during the descending stage of the suction nozzle. can be collected by suction.
（へ）実施例
以下、添付図面を参照して、本発明の液体試料採取方法
の実施の態様の一例を説明するが、本発明は、以下の説
明及び例示によって何ら限定されるものではない。(F) Example Hereinafter, an example of an embodiment of the liquid sample collection method of the present invention will be described with reference to the accompanying drawings, but the present invention is not limited in any way by the following description and exemplification.
図は、本発明の液体試料採取方法の一実施例の概略を示
す構造図である。The figure is a structural diagram schematically showing an embodiment of the liquid sample collection method of the present invention.
図において、キャピラリ試料容器１は、下端が栓２で密
封されており、上ｊ！１３はホルダ４に密着して袋ナツ
ト５により締着されている。ホルダ４は固定台６に取付
けられている。In the figure, a capillary sample container 1 is sealed at the lower end with a stopper 2, and at the upper end j! 13 is in close contact with the holder 4 and is fastened with a cap nut 5. The holder 4 is attached to a fixed base 6.
試料吸引ノズル７は、ノズルホルダ８に取付けられてお
り、このノズルホルダの１４９は、垂直に設けられてい
るラック１０１：！結しており、ラック１０と共に往復
動するようになっている。ラック１０は適宜の固定手段
（し１示されていない。）により固定されている案内部
材１１に滑動可能に設けζ）れている、このラック１０
に係合するビニオン１２は、所定位置に固設されている
第一のステッピングモータ１３の回転軸１４に取付けら
れており、ラック１０は第一のステッピングモータ１３
の回転により案内部材１１に沿って往復動する。The sample suction nozzle 7 is attached to a nozzle holder 8, and the nozzle holder 149 is connected to a vertically provided rack 101:! It is designed to reciprocate together with the rack 10. The rack 10 is slidably mounted on a guide member 11 fixed by suitable fixing means (not shown).
The pinion 12 that engages with the first stepping motor 13 is attached to a rotation shaft 14 of a first stepping motor 13 fixed at a predetermined position, and the rack 10 is connected to the first stepping motor 13.
The rotation causes the guide member 11 to reciprocate along the guide member 11.
試料吸引ノズル７は、管部材１５を介して適宜の固定手
段（図示されていない。）により固定されているピペッ
タ１６に接続している。ピペッタ１６のブランツヤ１７
の端部は、ラック１８に連結しており、ラック１８に伴
って往復動する。ラック１８は、滑動可能に適宜の固定
手段（図示されでいない、）により固定されている案内
部材１９に取付けられており、これに係合するビニオン
２０の回転により案内部材１９に沿って往復動する。The sample suction nozzle 7 is connected via a tube member 15 to a pipetter 16 which is fixed by suitable fixing means (not shown). Pipetta 16 Brantsuya 17
The end portion of is connected to the rack 18 and reciprocates with the rack 18. The rack 18 is slidably attached to a guide member 19 fixed by suitable fixing means (not shown), and is reciprocated along the guide member 19 by rotation of a pinion 20 engaged therewith. do.
ビニオン２０は所定位置に固設されている第二のステッ
ピングモータ２１の回転軸２２に固定されており、回転
軸の回転と共に回転する。第一のステッピングモータ１
３と第二のステッピングモータ２１は、信号線２３を介
して同期装？ｔ（図示されていない。）に接続しておワ
、試料吸引ノズル７の下降により、試料吸引ノズル７が
、キャビラリ試料容器１内に挿入され、その先端２４が
、試料ｆｉ２５に、例えば２〜３ ｔｘ浸漬したところ
で、第二のステッピングモータ２１が作動して、試料吸
引が開始されるようになっている。The binion 20 is fixed to a rotating shaft 22 of a second stepping motor 21 fixed at a predetermined position, and rotates with the rotation of the rotating shaft. First stepping motor 1
3 and the second stepping motor 21 are synchronized via the signal line 23. t (not shown), the sample suction nozzle 7 is inserted into the cavity sample container 1 by lowering the sample suction nozzle 7, and its tip 24 is inserted into the sample fi 25, for example, from 2 to After 3 tx immersion, the second stepping motor 21 is activated and sample suction is started.
本例は、以上のように構成されているので、第一ステッ
ピングモーフ１３を作動させて、ビニオン１２を回転さ
せ、ラック１０を下方に移動させる。このラック１０の
移動と共に、試料吸引／７：ル７は、ホルダ４の案内孔
２６を通して、キャピラリ試料容器１内に挿入される。Since this example is configured as described above, the first stepping morph 13 is operated to rotate the binion 12 and move the rack 10 downward. Along with this movement of the rack 10, the sample suction tube 7 is inserted into the capillary sample container 1 through the guide hole 26 of the holder 4.
試料吸引ノズル７の先端２４が、試料液２５内の一定深
さに浸漬されたところを、第一ステッピングモーフ１３
に内蔵される位置検出センサ（図示されていない。）に
より検出して、その検出信号を、第ニスチッピングモー
タ２１に信号＃ａ２３を介して送り、第ニスチッピング
モータ２１を作動させる。第ニスチッピングモータの作
動で、ビニオン２０が回転し、ラック１８を１９′側に
移動させる。このラック１８の移動と共にピペッタ１６
のプランツヤ１７は吸引側に移動して、試料吸引７ズル
７からの吸引を開始する。このピペッタ１６の吸引動作
の進行と共に、第一ステッピングモーフ１３の回転は、
継続されるので、試料吸引ノズルは、試料液２５を吸引
しながら、キャピラリ試料容器１内を下降する。プラン
ツヤ１７の吸引側の移動が一定位置に達したところを、
第ニステアピングモータ２１に内蔵の位置検出センサ（
図示されていない。）で検出して、第二ステッピングモ
ーフ２１の作動を停止させると共に、この停止信号を第
一ステッピングモーフ１３に送って、第一ステッピング
モーフ１３の回転を逆転させ、ラック１０を上方に移動
させる。一定量の試料液２５を吸引した試料吸引ノズル
は、ラック１０と共に上方に移動し、所定の位置に上昇
したところで、第一ステッピングモーフ１３に内蔵の位
置検出センサでこれを検出して、第一ステッピングモー
フ１３の作動を停止する。When the tip 24 of the sample suction nozzle 7 is immersed in the sample liquid 25 to a certain depth, the first stepping morph 13
The detection signal is detected by a position detection sensor (not shown) built in the varnish chipping motor 21, and the detection signal is sent to the varnish chipping motor 21 via the signal #a23, thereby operating the varnish chipping motor 21. The operation of the second varnish chipping motor causes the binion 20 to rotate and move the rack 18 to the 19' side. As the rack 18 moves, the pipetter 16
The planter 17 moves to the suction side and starts suctioning from the sample suction nozzle 7. As the suction operation of the pipettor 16 progresses, the rotation of the first stepping morph 13 is as follows:
As the sample suction nozzle continues, the sample suction nozzle descends inside the capillary sample container 1 while sucking the sample liquid 25. When the movement of the suction side of the planter 17 reaches a certain position,
The position detection sensor built into the second steering motor 21 (
Not shown. ), the operation of the second stepping morph 21 is stopped, and this stop signal is sent to the first stepping morph 13 to reverse the rotation of the first stepping morph 13 and move the rack 10 upward. The sample suction nozzle that has sucked a certain amount of the sample liquid 25 moves upward together with the rack 10, and when it rises to a predetermined position, it is detected by the position detection sensor built into the first stepping morph 13 and the first stepping morph 13 is moved upward. The operation of the stepping morph 13 is stopped.
第一ステッピングモーフ１３のこの作動停止のイパ号は
、試料吸引ノズル７を水平方向に移動させる移動装置の
ステッピングモータ（いずれも図示されていない。）に
送られて、試料吸引ノズル７を試料吐出位置に移動させ
る。This signal to stop the operation of the first stepping morph 13 is sent to a stepping motor (none of which is shown) of a moving device that moves the sample suction nozzle 7 in the horizontal direction, and the sample suction nozzle 7 is moved to the sample discharge position. move to position.
試料吸引ノズル７が、試料吐出位置への移動を終えたと
ころで、この位置検出信号を受けて、第一ステッピング
モーフ１３は、正転し、試料吸引ノズル７を一定位置ま
で下降させる。試料吸引ノズルマの下降の１８！、出侶
号を受けて、第二人チッピングモータ２１は、前の吸引
時とは、逆方向に回転して、ビニオン２０を吸引時とは
１匹の方向に回転させる。このビニオン２０の逆転によ
り、ラック】８は１９＃側に移動して、プランツヤ１７
を吐出側に移動させ、試料吐出位置に配置される分析用
の試料容器又は試料導入口に試料を吐出する。When the sample suction nozzle 7 has finished moving to the sample discharge position, upon receiving this position detection signal, the first stepping morph 13 rotates forward and lowers the sample suction nozzle 7 to a certain position. 18 of lowering the sample suction nozzle! In response to this, the second person's chipping motor 21 rotates in the direction opposite to the direction of the previous suction, and rotates the pinion 20 in the direction of one fish compared to the direction of the previous suction. Due to this reversal of the pinion 20, the rack]8 moves to the 19# side, and the plant rack 17
is moved to the discharge side, and the sample is discharged into a sample container for analysis or a sample inlet located at the sample discharge position.
試料の吐出を終えた試料吸引ノズル７は、洗浄位置に移
り、第ニスチッピングモータを正）立向方向に交互に作
動させて、試料吸引ノズル７の洗浄乾燥を行う。After the sample suction nozzle 7 has finished discharging the sample, the sample suction nozzle 7 is moved to the cleaning position, and the first varnish chipping motor is operated alternately in the vertical direction to clean and dry the sample suction nozzle 7.
洗浄乾燥された試料吸引ノズル７は、試料吸引位置２７
に移り、試料吸引ノズル７による試料吸引採取工程、試
料吐出工程及び試料吸引ノズルの洗浄乾燥工程の４作を
繰返す。The cleaned and dried sample suction nozzle 7 is moved to the sample suction position 27.
Then, four operations are repeated: a sample suction and collection process using the sample suction nozzle 7, a sample discharge process, and a sample suction nozzle cleaning and drying process.
本例によると、キャピラリ試料容器からの試料液の採取
が、空気を吸入することなく、容易に行うことがで外る
。According to this example, the sample liquid can be easily collected from the capillary sample container without inhaling air.
（ト）発明の効果
本発明は、液体試料採取用吸引ノズル開口部をえヤビラ
リ液体試料容器内の液体試料内に浸漬させ、続いて、該
吸引ノズル開口８ｂを下方に移動させながら液体試料の
吸引を行うので、キャピラリ液体試料容器から、液体試
料採取用吸引ノズルによる液体試料の採取が空気の吸入
を避けて正確に行うことができる。したがって、本発明
１よ、従来のキャピラリ液体試料容器からの成体試料採
取法に比して、空気の吸入等による試料採取ミスが少く
なり、信頼性の高い液体試料採取を行うことができ、延
いては、分析精度の向上に遮るものである。(G) Effects of the Invention In the present invention, the suction nozzle opening for liquid sample collection is immersed in the liquid sample in the liquid sample container, and then the suction nozzle opening 8b is moved downward while the liquid sample is collected. Since suction is performed, a liquid sample can be accurately collected from the capillary liquid sample container using the liquid sample collection suction nozzle without inhaling air. Therefore, in accordance with the present invention, compared to the conventional adult sample collection method from a capillary liquid sample container, sample collection errors due to air inhalation, etc. are reduced, and liquid sample collection can be performed with high reliability and extended. This hinders the improvement of analytical accuracy.
しかも、本発明は、このような信頼性の高い液体Ｋ ？
：４採取を行うので、従来法−二比して、ｔ’１重な徴
禁検体を無駄なく分析に供することができ、診断等の上
で頗る有益である。Moreover, the present invention provides such a highly reliable liquid K?
Since 4 samples are collected, compared to the conventional method, it is possible to use t'1 multiple samples for analysis without waste, which is extremely useful for diagnosis, etc.
図は、本発明の液体試料採取方法の一実施例の概略を示
す構造図である。
図中の符号については、１はキャピラリ試料容器、２は
栓、３は上端、４はホルダ、５は袋ナツト、６は固定台
、７は試料吸引ノズル、８はノズルホルダ、９はノズル
ホルダの他端、１０はラック、１１は案内部材、１２は
ビニオン、１３は第一のステッピングモータ、１４は回
転軸、１５は管部材、１６はビベフタ、１７はプランツ
ヤ、１８はラック、１９は案内部材、１９′及び１９″
はラックの移動方向、２０はビニオン、２１は第二のス
テッピングモータ、２２は回転軸、２３は信号線、２４
は試料吸引ノズルの先端、２５は試料液、２６は案内孔
、２７は試料吸引位置である。The figure is a structural diagram schematically showing an embodiment of the liquid sample collection method of the present invention. Regarding the symbols in the figure, 1 is the capillary sample container, 2 is the stopper, 3 is the upper end, 4 is the holder, 5 is the cap nut, 6 is the fixing base, 7 is the sample suction nozzle, 8 is the nozzle holder, and 9 is the nozzle holder. At the other end, 10 is a rack, 11 is a guide member, 12 is a pinion, 13 is a first stepping motor, 14 is a rotating shaft, 15 is a tube member, 16 is a bibe lid, 17 is a planter, 18 is a rack, and 19 is a guide Members, 19' and 19''
is the moving direction of the rack, 20 is the pinion, 21 is the second stepping motor, 22 is the rotating shaft, 23 is the signal line, 24
25 is the tip of the sample suction nozzle, 25 is the sample liquid, 26 is the guide hole, and 27 is the sample suction position.
Claims (1)
を挿入して、キャピラリ液体試料容器内に収容されてい
る液体試料を該吸引ノズル内に吸込み液体試料の採取を
行うキャピラリ液体試料容器からの液体試料採取方法に
おいて、少くとも液体試料採取用吸引ノズル開口部をキ
ャピラリ液体試料容器内の液体試料内に浸漬させ、続い
て、該吸引ノズル開口部を該液体試料内を下方に移動さ
せながら、該吸引ノズル内への液体試料の吸引を開始す
ることを特徴とするキャピラリ液体試料容器からの液体
試料採取方法。A liquid sample is collected from a capillary liquid sample container by inserting a suction nozzle for liquid sample collection into a capillary liquid sample container and sucking the liquid sample contained in the capillary liquid sample container into the suction nozzle. In the sampling method, at least a suction nozzle opening for liquid sample collection is immersed in a liquid sample in a capillary liquid sample container, and then, while the suction nozzle opening is moved downward within the liquid sample, the suction is performed. A method for collecting a liquid sample from a capillary liquid sample container, comprising starting suction of the liquid sample into a nozzle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19581786A JPS6350735A (en) | 1986-08-21 | 1986-08-21 | Method for sampling liquid specimen from capillary liquid specimen container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19581786A JPS6350735A (en) | 1986-08-21 | 1986-08-21 | Method for sampling liquid specimen from capillary liquid specimen container |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6350735A true JPS6350735A (en) | 1988-03-03 |
| JPH0454179B2 JPH0454179B2 (en) | 1992-08-28 |
Family
ID=16347484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19581786A Granted JPS6350735A (en) | 1986-08-21 | 1986-08-21 | Method for sampling liquid specimen from capillary liquid specimen container |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6350735A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008275353A (en) * | 2007-04-26 | 2008-11-13 | Nippon Koden Corp | Collection apparatus of trace amount of liquid, and adapter |
| JP2008292393A (en) * | 2007-05-28 | 2008-12-04 | Sysmex Corp | Collection tube selecting method and analyzing device management system |
| WO2009017065A1 (en) * | 2007-07-31 | 2009-02-05 | Olympus Corporation | Analyzing device, rinsing device, dispensing device, agitating device, and container |
| CN109916676A (en) * | 2019-03-28 | 2019-06-21 | 国家海洋环境监测中心 | A kind of seawater quality sampling apparatus that marine monitoring depth is different |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5339185A (en) * | 1976-09-21 | 1978-04-10 | Omron Tateisi Electronics Co | Separately sampling apparatus of blood serum |
| JPS5977067U (en) * | 1982-11-16 | 1984-05-24 | 株式会社島津製作所 | Capillary type sampling device |
| JPS6013241A (en) * | 1983-07-04 | 1985-01-23 | Shimadzu Corp | Auto-sampler |
-
1986
- 1986-08-21 JP JP19581786A patent/JPS6350735A/en active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5339185A (en) * | 1976-09-21 | 1978-04-10 | Omron Tateisi Electronics Co | Separately sampling apparatus of blood serum |
| JPS5977067U (en) * | 1982-11-16 | 1984-05-24 | 株式会社島津製作所 | Capillary type sampling device |
| JPS6013241A (en) * | 1983-07-04 | 1985-01-23 | Shimadzu Corp | Auto-sampler |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008275353A (en) * | 2007-04-26 | 2008-11-13 | Nippon Koden Corp | Collection apparatus of trace amount of liquid, and adapter |
| JP2008292393A (en) * | 2007-05-28 | 2008-12-04 | Sysmex Corp | Collection tube selecting method and analyzing device management system |
| WO2009017065A1 (en) * | 2007-07-31 | 2009-02-05 | Olympus Corporation | Analyzing device, rinsing device, dispensing device, agitating device, and container |
| CN109916676A (en) * | 2019-03-28 | 2019-06-21 | 国家海洋环境监测中心 | A kind of seawater quality sampling apparatus that marine monitoring depth is different |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0454179B2 (en) | 1992-08-28 |
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