JPS617466A - Method and apparatus for filling of column in liquid chromatograph - Google Patents

Method and apparatus for filling of column in liquid chromatograph

Info

Publication number
JPS617466A
JPS617466A JP59126361A JP12636184A JPS617466A JP S617466 A JPS617466 A JP S617466A JP 59126361 A JP59126361 A JP 59126361A JP 12636184 A JP12636184 A JP 12636184A JP S617466 A JPS617466 A JP S617466A
Authority
JP
Japan
Prior art keywords
column
filling
movable stopper
pressure
plug
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP59126361A
Other languages
Japanese (ja)
Other versions
JPH0372947B2 (en
Inventor
Sadao Kuroiwa
黒岩　貞雄
Isao Saito
勲 斉藤
Norio Ishida
石田　典男
Tsuyoshi Yamada
強 山田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Resonac Holdings Corp
Original Assignee
Showa Denko KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Showa Denko KK filed Critical Showa Denko KK
Priority to JP59126361A priority Critical patent/JPS617466A/en
Publication of JPS617466A publication Critical patent/JPS617466A/en
Publication of JPH0372947B2 publication Critical patent/JPH0372947B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/522—Physical parameters pressure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods
    • G01N2030/562—Packing methods or coating methods packing

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

PURPOSE:To improve separation efficiency of a subjected material, by fitting under pressure a movable plug mounted on a column inside the column with a mechanical transmission mechanism and charging the column with a filling material under pressure. CONSTITUTION:After pouring concentration adjusted slurry filling material into a column 1 fixed from above onto a supporting bench 6, a movable plug 2 is fit and further, an electric motor 5 is driven to a constant speed by setting a travelling speed of the movable plug, allowing the plug 2 to drop through reduction gear 7, rotating shafts 4A, 4B. Thus, the poured slurry of the filling material is filled under a constant load and when the set load is found in excess of the set value in the neighborhood of the completed state of filling, the motor 5 is brought to a stand still to finish up the filling operation. As a reduction ratio of the reduction gear 7 is set in a wide range for the movable plug 2 of the column, the plug 2 is not subjected to any movement by fluid pressure at the time of analysis or blelding, etc., and consequently, the column can be used as a backed column even if the pressure is approximately 200kg/cm<2>G.

Description

【発明の詳細な説明】 ３．１　　技術分野 本特許は、液体クロマトグラフの主要部分であるカラム
に対象とする成分物質の分離に適した充填剤を充填する
方法およびその装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION 3.1 Technical Field The present patent relates to a method and apparatus for filling a column, which is a main part of a liquid chromatograph, with a packing material suitable for separating target component substances.

３．２　　従来技術とその問題点 従来の液体クロマトグラフにおいては、分離カラムとし
て大部分があらかじめ充填された市販のパンクドカラム
により限られた充填剤による限られた方法のカラムを使
用しているが、最近は液体クロマトグラフによる分離の
技術をベースとして生化学物質や医薬品の大量分取を行
なうことが志向され、より効率よくこれを行なうために
、対象とする物質の分離に最適な充填剤を用い、適切な
方法で充填したカラムにより、その分離効率を高めるこ
とができる充填装置を備えた液体クロマトグラフが使用
されるようになってきている。3.2 Prior art and its problems Conventional liquid chromatographs use columns with limited packing material and limited method as separation columns, mostly pre-packed commercially available punctured columns. However, in recent years, there has been a trend toward bulk separation of biochemical substances and pharmaceuticals based on liquid chromatography separation technology, and in order to do this more efficiently, we have developed packing materials that are optimal for separating the target substances. Liquid chromatographs equipped with packing devices that can increase separation efficiency by using columns packed in an appropriate manner have come into use.

しかしこれまで使用されてきた充填装置では次にあげる
ような問題点があり、使用する側からは不都合なものと
なっている。However, the filling devices that have been used so far have the following problems, making them inconvenient for users.

（１）流体圧力を利用した充填剤の入ったカラムに外部
から加圧し内容物の充填密度を上げる方式のものであり
、使用圧力とのバランスの関係で低圧側を可動栓として
充填されるべき充填剤全体を可動栓により移動し、固定
壁に対して加圧充填するものとなっている。(1) This method uses fluid pressure to pressurize the column containing the packing material from the outside to increase the packing density of the contents.In relation to the balance with the working pressure, the low pressure side should be filled with a movable stopper. The entire filling material is moved by a movable stopper and is filled under pressure against a fixed wall.

この場合、充填される充填剤の粒子全部が移動するため
、粒子とカラム壁又は粒子同志の摩擦が起り、吸着性能
を劣化させ理論段数を極端に低下させる。In this case, all the packed particles of the packing material move, causing friction between the particles and the column wall or between particles, which deteriorates the adsorption performance and extremely reduces the number of theoretical plates.

（２）流体圧力はカラム使用時の低圧側を可動栓として
いるが、分析又は分取においてサンプルまたは溶離液を
加圧して流すため、可動栓に内部から力が加わることと
なる。したがってこの方法による充填装置では、使用時
には常時充填圧力である流体圧をかけておくことが必要
となる。(2) For fluid pressure, a movable stopper is used on the low-pressure side when the column is used; however, in analysis or preparative separation, the sample or eluent is flowed under pressure, so force is applied to the movable stopper from within. Therefore, in the filling device using this method, it is necessary to constantly apply fluid pressure, which is the filling pressure, during use.

（３）Ｆ記（＋）、（２）の状況において可動栓の移動
荷重にむらや無理がかかり一定荷重、一定荷重での充填
ができない。また流体圧力による可動栓の移動を防止す
るためにさらに外部より加圧［７なければならない。し
たがって使用圧力に２０Ｋｇ／　ｃ　ｍ’Ｇ以下という
制限が必要である。しかしながら２０Ｋｇ／　ｃ　ｍ’
程度の圧力では充分な機能発揮はできない。すなわち充
填剤の充填密度もあげられず理論段数の向上もできない
。(3) In the situations described in F (+) and (2), the moving load of the movable stopper becomes uneven or unreasonable, making it impossible to fill with a constant load. Further, in order to prevent movement of the movable stopper due to fluid pressure, additional pressure must be applied from the outside [7]. Therefore, it is necessary to limit the working pressure to 20 kg/cm'G or less. However, 20Kg/cm'
Sufficient functionality cannot be achieved with a certain amount of pressure. That is, the packing density of the filler cannot be increased, and the number of theoretical plates cannot be improved either.

３．３　　本発明の目的 ここに提示する方法および装置は、これらの問題点を解
決し、経験の浅い人でも簡単に充填剤の充填ができ、し
かも理論段数の向上にもつながる液体クロマトグラフ用
カラム充填の方法およびその装置である。3.3 Purpose of the present invention The method and apparatus presented here solve these problems, and are suitable for use in liquid chromatographs, allowing even inexperienced people to easily fill the packing material, and also leading to an increase in the number of theoretical plates. A method and apparatus for column packing.

３．４　　本発明の構成 本発明においては基本的形態としてカラムの−・方の入
口に装着した可動栓を機械的伝導機構によりカラムの内
側に圧入することにより、充填剤を加圧することを特徴
とする液体クロマトグラフ用カラムの充填方法が提供さ
れ、更に又、可動栓と、可動栓をカラム内側に圧入する
雷ための機械的伝導機構とカラムを固定する受台とを有
することを特徴とする液体クロマトグラフ用カラムの充
填装置が提供される。3.4 Structure of the present invention The basic feature of the present invention is that the packing material is pressurized by pressurizing a movable plug attached to the - side inlet of the column into the inside of the column using a mechanical conduction mechanism. A method for filling a column for liquid chromatography is provided, further comprising a movable stopper, a mechanical conduction mechanism for press-fitting the movable stopper into the inside of the column, and a pedestal for fixing the column. A packing device for a liquid chromatography column is provided.

本発明により提供される装置は可動栓方式又は可動カラ
ム方式により高効率充填の機構を備え、可動栓部はシー
ル部の材質、形状を耐薬品性、耐摩耗性かつ好ましくは
スクレーパー構造とし、耐圧２００Ｋｇ／　ｃ　ｍ’Ｇ
以上のシール性が高い構造である。又、充填剤注入や機
構部保守のため可動栓部を着脱可能な構造とし、更に充
填に際しては一定荷重、−・定荷重を確保することがで
きるものである。The device provided by the present invention is equipped with a highly efficient filling mechanism using a movable plug system or a movable column system, and the movable plug part has a sealing part made of a material and shape that is chemical-resistant, wear-resistant, and preferably has a scraper structure, and has a pressure-resistant structure. 200Kg/cm'G
This structure has a high sealing performance. In addition, the movable plug part is designed to be removable for injecting the filler and maintaining the mechanism, and furthermore, it is possible to ensure a constant load during filling.

以丁図面を参照しつつ、本発明の詳細な説明する。説明
の便宜上、−具体例を示すが、勿論本発明の構成はこれ
に限定される訳ではない。The present invention will now be described in detail with reference to the drawings. For convenience of explanation, a specific example will be shown, but of course the configuration of the present invention is not limited to this.

第１図は本発明に係る充填剤充填装置を示す概略構成図
であって液体クロマトグラフカラム１をカラム受台８に
取付は固定させる。この状態では可動栓２は取外してあ
り、可動栓移動台座３は一番上にあがっていて、この時
は制限用スイッチが働いており、これ以上の上昇はでき
ないよう電気的機械的に配慮されている。１度調整され
たスラリー状の充填剤をカラム１上部よりカラムｌに注
入して可動栓２を装着し、可動栓２は可動栓移動台座３
に固定する。そして可動栓移動荷重を設定して電動機５
を定速で回転させ減速機構７を経て可動栓２と可動栓移
動台座３は電動機５の回転にあわせて回転軸４＾、４Ｂ
がまわることにより上昇または下降する。注入された充
填剤のスラリーは−・定荷重で充填される。充填完了付
近では予め設定された荷重を越えた時、制限荷重超過に
より電動機５が伴出して充填が完了する。木カラムの可
動栓２は減速機構７の減速比を大きくとっであるために
分析または分取等の使用時の流体圧によって可動栓２が
移動することのないようにしであるため、２００　Ｋｇ
／　ｃ　ｍ’Ｇ程度の圧力でもバツクドカラム同様の使
用ができる。FIG. 1 is a schematic configuration diagram showing a packing material filling apparatus according to the present invention, in which a liquid chromatography column 1 is mounted and fixed on a column holder 8. In FIG. In this state, the movable stopper 2 has been removed and the movable stopper moving base 3 has risen to the top, and at this time, the limit switch is activated and electrical and mechanical considerations are taken to prevent it from rising any further. ing. The slurry-like packing material that has been adjusted once is injected into the column 1 from the top of the column 1, and the movable stopper 2 is attached.
Fixed to. Then, the movable stopper moving load is set and the electric motor 5
The movable stopper 2 and the movable stopper moving base 3 are rotated at a constant speed through the deceleration mechanism 7, and the movable stopper 2 and the movable stopper moving base 3 are rotated along the rotation shafts 4^, 4B in accordance with the rotation of the electric motor 5.
It rises or falls by rotating. The injected filler slurry is filled with a constant load. Near the completion of filling, when the preset load is exceeded, the electric motor 5 is driven out due to the excess of the limit load, and the filling is completed. The movable stopper 2 of the wooden column has a large reduction ratio of the reduction mechanism 7, so that the movable stopper 2 does not move due to fluid pressure during analysis or preparative use, so it weighs 200 kg.
It can be used in the same way as a backed column even at a pressure of about /cm'G.

充填された充填剤が使用済みとなった時、カラム１底部
より抜き出し、抜き終ったら可動栓２を可動栓移動台座
３より外し、次の充填にそなえる。When the filled packing material is used up, it is extracted from the bottom of the column 1, and when the filling is finished, the movable stopper 2 is removed from the movable stopper moving base 3 to prepare for the next filling.

第２図は第１図により説明した装置において可動栓とカ
ラムぐまたは可動カラムと固定柱）とが相対的に移動す
るにもかかわらず、内部のスラリーを一定荷重または一
定荷重で充填することを可能とするためのシール部およ
びスクレーパーの構成を示すものである。Fig. 2 shows that in the apparatus explained in Fig. 1, the internal slurry is filled with a constant load or a constant load even though the movable stopper and the column (or the movable column and the fixed column) move relative to each other. This figure shows the configuration of the seal part and scraper to make this possible.

本発明で採用したシール構造は、従来法ではスラリー中
の粒状物質が０−リングとカラム壁の間に入り、シール
性能を悪化させ、０−リングの寿命を短＜＜シていると
いう問題があるのに対し、０−リングとスクレーパーリ
ングを組合わせ、かつ上昇下降いずれへ移動する場合に
もシール性を確保できるようにスペーサをはさんで上下
に２組を配置した構造となっている。The seal structure adopted in the present invention has the problem that in the conventional method, particulate matter in the slurry gets between the O-ring and the column wall, deteriorating the sealing performance and shortening the life of the O-ring. On the other hand, it has a structure in which an O-ring and a scraper ring are combined, and two sets are arranged above and below with a spacer in between to ensure sealing performance when moving upward or downward.

第２図において可動栓２が下降し、カラム内のスクリュ
ーに圧力をかけ粒状物質の均一な充填を行なう場合、カ
ラム内のスラリーに圧力がかかることにより排出される
液は、フィルター１６で分離され、継手１７を介して管
１８から外部へ排出される。In FIG. 2, when the movable stopper 2 descends and applies pressure to the screw in the column to uniformly fill the particulate matter, the liquid discharged due to the pressure applied to the slurry in the column is separated by the filter 16. , are discharged to the outside from the pipe 18 via the joint 17.

５ｆ動栓先端部１２は、スラリーに圧力をかけながら下
方へ移動し、スラリーはカラム管壁と可動栓先端部１２
との間の狭い部分に侵入するが、液は０−リング１４゛
　により、また粒状物質はスクレーパー１５’　により
ミールされなからカラム内で充填される。The 5f movable stopper tip 12 moves downward while applying pressure to the slurry, and the slurry moves between the column tube wall and the movable stopper tip 12.
However, the liquid is not milled by the O-ring 14' and the particulate matter is not milled by the scraper 15', and then packed into the column.

この場合、スクレーパー１５°は０−リング１４°より
下側に配置されているため、０−リングとカラム壁の間
に粒状物質が侵入することを完全に防止することができ
る。In this case, since the scraper 15° is disposed below the 0-ring 14°, it is possible to completely prevent particulate matter from entering between the 0-ring and the column wall.

他方、可動栓が上昇する場合も０−リング１４のシール
性を確保するためにスクレーパー１５を上方に配置し、
完全なシール性を保持することができるようになってい
る。On the other hand, in order to ensure the sealing performance of the O-ring 14 even when the movable stopper is raised, the scraper 15 is placed above.
It is now possible to maintain perfect sealing performance.

３．５　　本発明による実施例 次に本発明によるカラム充填装置を用いて液体クロマト
グラフ用カラムにイオン交換を主たる性能とする樹脂ゲ
ル（ｒｏｎｐａｋ　ｋｓ−８０２用ゲル）を充填し、オ
リゴ糖をサンプルとし各種糖の分取を行なった場合を実
施例として説明する。3.5 Example according to the present invention Next, using the column packing apparatus according to the present invention, a resin gel whose main performance is ion exchange (gel for Ronpak KS-802) was packed into a liquid chromatography column, and an oligosaccharide was sampled. An example will be described in which various types of sugars are separated.

３．５．１理論段数 ２０ｍｍφ、５０）φそれぞれのカラムに上記ゲルを充
填した場合、充填荷重を２０〜１００ｍ１ｌ／ｍｉｎの
範囲で変化させ理論段数を測定した結果を第３図に示す
。3.5.1 Number of Theoretical Plate When the gel was packed into each of the columns of 20 mmφ and 50 mmφ, the number of theoretical plates was measured while changing the packing load in the range of 20 to 100 ml/min. The results are shown in FIG. 3.

第３図において５ｈａφカラムでは２５ｍｍ／ｍｉｎの
充填荷重で１３，０００段／ｍ　、　２０ｍｍφカラム
では９０ｍｍ／ｗｉｎの充填荷重で２２．０００段／ｍ
の理論段数となる。In Figure 3, a 5haφ column has a packing load of 25mm/min and a packing load of 13,000 plates/m, and a 20mmφ column has a packing load of 90mm/win and a packing load of 22,000 plates/m.
The number of theoretical plates is .

ちなみに、気体加圧方式による従来法では充填圧力に制
限があり、約４，０００段／膳である。Incidentally, in the conventional method using the gas pressurization method, there is a limit to the filling pressure, which is about 4,000 plates/plate.

３．５．２オリゴ糖の分離分取性能 第４図は従来法により８０ｍｍφカラムにゲルを充填し
た場合の分取結果を示す。3.5.2 Oligosaccharide separation/preparation performance Figure 4 shows the preparative separation results when gel was packed into an 80 mmφ column using the conventional method.

この場合、使用圧力上限が２０ｋｇ／　ｃ　ｍ’Ｇのた
め溶層液流量１００ｍ１　／　ｌｌ１ｉｎが最高であり
、理論段数７．０００段／ｆｆｌで分取完了までの時間
は約１０分を要する。In this case, since the upper limit of the working pressure is 20 kg/cm'G, the maximum flow rate of the solution solution is 100 m1/11 in, and the number of theoretical plates is 7,000 plates/ffl, and it takes about 10 minutes to complete the fractionation.

第５図は本発明による方法により８０ｍｍφカラムに同
一ゲルを充填し、従来法と同一条件で分取を行なった場
合のクロマトグラフである。溶離液流量を　１００１／
ｍｉｎとした場合、圧力は３０ｋｇ／　ｃ　ｍ’Ｇとな
り、理論段数的２０．０００段／Ｉ１１であり、分離性
能は従来法より向上していると云える。FIG. 5 is a chromatograph obtained by filling an 80 mmφ column with the same gel according to the method of the present invention and performing fractionation under the same conditions as the conventional method. Eluent flow rate 1001/
When the pressure is 30 kg/cm'G, the number of theoretical plates is 20,000 plates/I11, and it can be said that the separation performance is improved compared to the conventional method.

第６図は第５図と同一カラムにおいて従来法では使用不
可能な圧力９０ｋｇ／ｃｍ’Ｇまでの加圧を行ない、溶
離液流量を３００＋＋＋Ｉ／ｍｉｎとした場合のクロマ
トグラフである。FIG. 6 is a chromatograph obtained when the same column as in FIG. 5 was pressurized to a pressure of 90 kg/cm'G, which cannot be used in the conventional method, and the eluent flow rate was set to 300+++ I/min.

この場合、分取完了までの時間が約３分となり、分離性
能は第５図の場合と同等、すなわち従来法より相当の向
上がある。In this case, it takes about 3 minutes to complete the separation, and the separation performance is the same as in the case of FIG. 5, that is, a considerable improvement over the conventional method.

３．６　　本発明の効果 本発明の方法および装置により液体クロマトグラフ用カ
ラムの充填が極めて容易になり、同時にこのように充填
されたカラムの理論段数が向にする。3.6 Effects of the present invention The method and apparatus of the present invention make it extremely easy to pack a column for liquid chromatography, and at the same time increase the number of theoretical plates in a column packed in this way.

【図面の簡単な説明】[Brief explanation of drawings]

第１図は本発明に係る充填剤装置の概略構成図、第２図
は同シール部およびスクレーパーの構成図、第３図はカ
ラムの充填荷重とその理論段数との関係図、第４図は従
来法により充填したカラムによる分取結果を示す図、第
５図、第６図は本発明により充填したカラムによる分取
結果を示す図である。 １−カラム、２−可動栓、３−可動栓移動台座、４Ａ　
、　４Ｂ−回転軸、５−電動機、８−カラム受台、７−
減速機構、１１−可動栓軸、１２−可動栓先端部、１３
−スペーサー、１４．＋４°−０−リング、１５．１５
−スクレーパー、１６−フィルター、１？−７７１手、
１８−管特許出願人　　昭和電工株式会社 代　理　人　　弁理士　菊地精− 第３図 第４図 ＥＬｕｅｎｔ　　：　Ｈ２Ｃ ＦＩＯｗｒａｔｅ　＋　１００　”’／＝ｎＰｒｅｓｓ
ｕｒｅ　：　２０　ｋｇ／ｃｎ２ＱＳａｒｎｐｔｅ　：
　ＯＬｉｇｏｓＣＬｃｃｈｃＬｒｔｄｅ５０　　　　２
．５．５　　　　７．５　　　１０（ｍｍ） （ｍｉｎ） （ｆｆＬＬｒＬ） Ｐ統補正書　（方式） 昭和５９年ｌρ月ｆ日Figure 1 is a schematic configuration diagram of the packing device according to the present invention, Figure 2 is a configuration diagram of the seal section and scraper, Figure 3 is a diagram showing the relationship between column packing load and the number of theoretical plates, and Figure 4 is a diagram of the relationship between column packing load and the number of theoretical plates. A diagram showing the results of fractionation using a column packed according to the conventional method, and FIGS. 5 and 6 are diagrams showing the results of fractionation using the column packed according to the present invention. 1-column, 2-movable stopper, 3-movable stopper moving base, 4A
, 4B-rotating shaft, 5-electric motor, 8-column pedestal, 7-
Reduction mechanism, 11-movable stopper shaft, 12-movable stopper tip, 13
-Spacer, 14. +4°-0-ring, 15.15
-Scraper, 16-Filter, 1? -771 moves,
18-Manufacturing patent applicant Showa Denko Co., Ltd. Representative Patent attorney Sei Kikuchi- Figure 3 Figure 4 ELuent: H2C FIOwrate + 100 ”'/=nPress
ure: 20 kg/cn2Q Sarnpte:
OLigosCLcchcLrtde50 2
．． 5.5 7.5 10 (mm) (min) (ffLLrL) P system amendment (method) 1985 lρ month f day

Claims (1)

【特許請求の範囲】 １、カラムの一方の入口に装着した可動栓を機械的伝導
機構によりカラムの内側に圧入することにより、充填剤
を加圧することを特徴とする液体クロマトグラフ用カラ
ムの充填方法。 ２、可動栓と、可動栓をカラム内側に圧入するための機
械的伝導機構とカラムを固定する受台とを有することを
特徴とする液体クロマトグラフ用カラムの充填装置。 ４、圧入荷重が一定であることを特徴とする特許請求の
範囲第１項記載の液体クロマトグラフ用カラムの充填方
法。 ５、可動栓にスクレーパーを有することを特徴とする特
許請求の範囲第２項記載の液体クロマトグラフ用カラム
の充填装置。[Claims] 1. Packing of a column for liquid chromatography, characterized in that a movable stopper attached to one inlet of the column is pressurized into the inside of the column by a mechanical conduction mechanism to pressurize the packing material. Method. 2. A liquid chromatography column packing device characterized by having a movable stopper, a mechanical conduction mechanism for press-fitting the movable stopper into the column, and a pedestal for fixing the column. 4. The method for packing a column for liquid chromatography according to claim 1, wherein the press-fitting load is constant. 5. The packing device for a column for liquid chromatography according to claim 2, characterized in that the movable stopper has a scraper.
JP59126361A 1984-06-21 1984-06-21 Method and apparatus for filling of column in liquid chromatograph Granted JPS617466A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP59126361A JPS617466A (en) 1984-06-21 1984-06-21 Method and apparatus for filling of column in liquid chromatograph

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59126361A JPS617466A (en) 1984-06-21 1984-06-21 Method and apparatus for filling of column in liquid chromatograph

Publications (2)

Publication Number Publication Date
JPS617466A true JPS617466A (en) 1986-01-14
JPH0372947B2 JPH0372947B2 (en) 1991-11-20

Family

ID=14933280

Family Applications (1)

Application Number Title Priority Date Filing Date
JP59126361A Granted JPS617466A (en) 1984-06-21 1984-06-21 Method and apparatus for filling of column in liquid chromatograph

Country Status (1)

Country Link
JP (1) JPS617466A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0649019A1 (en) * 1993-10-19 1995-04-19 Labomatic Instruments Ag Axially compressible device for chromatography
WO1997032207A1 (en) * 1996-02-29 1997-09-04 Varian Associates, Inc. Collapsible apparatus for compressing packing material in liquid chromatography columns and methods of use
JP2009512840A (en) * 2005-10-21 2009-03-26 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ Chromatographic column automatic packing apparatus and automatic packing method
KR20190084524A (en) * 2018-01-08 2019-07-17 (주) 영진바이오크롬 Packing system and method of preparative hplc column

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57183559U (en) * 1981-05-15 1982-11-20

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57183559U (en) * 1981-05-15 1982-11-20

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0649019A1 (en) * 1993-10-19 1995-04-19 Labomatic Instruments Ag Axially compressible device for chromatography
WO1997032207A1 (en) * 1996-02-29 1997-09-04 Varian Associates, Inc. Collapsible apparatus for compressing packing material in liquid chromatography columns and methods of use
JP2009512840A (en) * 2005-10-21 2009-03-26 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ Chromatographic column automatic packing apparatus and automatic packing method
JP4898819B2 (en) * 2005-10-21 2012-03-21 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ Chromatographic column automatic packing apparatus and automatic packing method
KR20190084524A (en) * 2018-01-08 2019-07-17 (주) 영진바이오크롬 Packing system and method of preparative hplc column

Also Published As

Publication number Publication date
JPH0372947B2 (en) 1991-11-20

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