JPS57159495A - Preparation of glucose - Google Patents
Preparation of glucoseInfo
- Publication number
- JPS57159495A JPS57159495A JP4549881A JP4549881A JPS57159495A JP S57159495 A JPS57159495 A JP S57159495A JP 4549881 A JP4549881 A JP 4549881A JP 4549881 A JP4549881 A JP 4549881A JP S57159495 A JPS57159495 A JP S57159495A
- Authority
- JP
- Japan
- Prior art keywords
- glucoamylase
- immobilized
- soluble
- starch
- pullulanase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To prepare the titled substance in high yield, by hydrolyzing a partially hydrolyzed starch with a soluble glucoamylase and a soluble α-amylase, and reacting the resultant hydrolyzate with an immobilized pullulanase and immobilized glucoamylase.
CONSTITUTION: A hydrolyzed starch, having a glucose equivalent of 10W30, and obtained from a starch of corn, potato, etc. is reacted in the presence of a soluble glucoamylase and soluble α-amylase at a pH of 4.5W7 to give a starch hydrolyzate with a glucose content of 20W85wt/vol% based on the total weight of saccharid. The resultant hydrolyzate is then adjusted to a solid content of 25W 55wt/vol% and then reacted in the presence an immobilized pullulanase at a pH of 5W7 and further in the presence of immobilized glucoamylase at a pH of 3.5W6. The immobilized enzymes are obtained by bonding pullulanase and glucoamylase to a granular gelatin with a polyfunctional crosslinking agent.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4549881A JPS57159495A (en) | 1981-03-30 | 1981-03-30 | Preparation of glucose |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4549881A JPS57159495A (en) | 1981-03-30 | 1981-03-30 | Preparation of glucose |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS57159495A true JPS57159495A (en) | 1982-10-01 |
Family
ID=12721062
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4549881A Pending JPS57159495A (en) | 1981-03-30 | 1981-03-30 | Preparation of glucose |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57159495A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002074895A3 (en) * | 2001-03-19 | 2003-06-12 | Novozymes As | Fermentation process including the use of enzymes |
-
1981
- 1981-03-30 JP JP4549881A patent/JPS57159495A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002074895A3 (en) * | 2001-03-19 | 2003-06-12 | Novozymes As | Fermentation process including the use of enzymes |
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