WO2002074895A3 - Fermentation process including the use of enzymes - Google Patents

Fermentation process including the use of enzymes Download PDF

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Publication number
WO2002074895A3
WO2002074895A3 PCT/DK2002/000179 DK0200179W WO02074895A3 WO 2002074895 A3 WO2002074895 A3 WO 2002074895A3 DK 0200179 W DK0200179 W DK 0200179W WO 02074895 A3 WO02074895 A3 WO 02074895A3
Authority
WO
WIPO (PCT)
Prior art keywords
amylase
fermentation
fermentation process
enzymes
process including
Prior art date
Application number
PCT/DK2002/000179
Other languages
French (fr)
Other versions
WO2002074895A2 (en
Inventor
Hans Sejr Olsen
Sven Pedersen
Robert Beckerich
Christopher Veit
Claus Felby
Original Assignee
Novozymes As
Novozymes North America Inc
Hans Sejr Olsen
Sven Pedersen
Robert Beckerich
Christopher Veit
Claus Felby
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As, Novozymes North America Inc, Hans Sejr Olsen, Sven Pedersen, Robert Beckerich, Christopher Veit, Claus Felby filed Critical Novozymes As
Priority to US10/472,256 priority Critical patent/US20040115779A1/en
Priority to EP02708252A priority patent/EP1373539A2/en
Priority to AU2002242633A priority patent/AU2002242633A1/en
Publication of WO2002074895A2 publication Critical patent/WO2002074895A2/en
Publication of WO2002074895A3 publication Critical patent/WO2002074895A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00Other raw materials for the preparation of beer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00Fermentation processes for beer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G1/00Preparation of wine or sparkling wine
    • C12G1/02Preparation of must from grapes; Must treatment and fermentation
    • C12G1/0203Preparation of must from grapes; Must treatment and fermentation by microbiological or enzymatic treatment
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a fermentation process, wherein the fermentation step is carried out in the presence of at least one carbohydrate-source generating enzyme activity (e.g. a glucoamylase, a beta-amylase or a maltogenic amylase) and at least one alpha-amylase activity. The process may additionally be carried out in the presence of a protease, a debranching enzyme activity and/or a yeast cell wast degrading enzyme. In one embodiment, the micro-organism used in the process is a yeast. The fermentation product may be for example ethanol, beer or wine.
PCT/DK2002/000179 2001-03-19 2002-03-19 Fermentation process including the use of enzymes WO2002074895A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/472,256 US20040115779A1 (en) 2002-03-19 2002-03-19 Fermentation process
EP02708252A EP1373539A2 (en) 2001-03-19 2002-03-19 Improved fermentation process
AU2002242633A AU2002242633A1 (en) 2001-03-19 2002-03-19 Fermentation process including the use of enzymes

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US27738301P 2001-03-19 2001-03-19
US27738401P 2001-03-19 2001-03-19
US60/277,384 2001-03-19
US60/277,383 2001-03-19
US30438001P 2001-07-10 2001-07-10
US60/304,380 2001-07-10

Publications (2)

Publication Number Publication Date
WO2002074895A2 WO2002074895A2 (en) 2002-09-26
WO2002074895A3 true WO2002074895A3 (en) 2003-06-12

Family

ID=27402901

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000179 WO2002074895A2 (en) 2001-03-19 2002-03-19 Fermentation process including the use of enzymes

Country Status (3)

Country Link
EP (1) EP1373539A2 (en)
AU (1) AU2002242633A1 (en)
WO (1) WO2002074895A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101545424B1 (en) 2004-05-27 2015-08-18 다니스코 유에스 인크. Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
US9234167B2 (en) 2009-03-03 2016-01-12 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004050820A1 (en) 2002-12-05 2004-06-17 Novozymes A/S Beer mashing process
ITMI20031926A1 (en) * 2003-10-07 2005-04-08 Etea S R L IMPROVED PROCEDURE FOR THE PREPARATION OF CEREAL ETHANOL.
US20070148741A1 (en) 2003-12-19 2007-06-28 Novozymes A/S Mashing process
US20070141688A1 (en) * 2004-02-19 2007-06-21 Novozymes North American, Inc. Liquefaction processes
RU2006144096A (en) * 2004-05-13 2008-06-20 Новозаймз Норт Америка, Инк. METHOD FOR PRODUCING FERMENTATION PRODUCT
WO2005118800A1 (en) 2004-05-27 2005-12-15 Genencor International, Inc. Heterologous expression of an aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
WO2005121305A1 (en) * 2004-06-08 2005-12-22 Novozymes A/S Mashing process
CN101115843A (en) * 2005-02-07 2008-01-30 诺维信北美公司 Fermentation product production processes
EP1941049A4 (en) 2005-09-20 2011-12-21 Novozymes North America Inc Process of producing a fermentation product
ES2298054B1 (en) * 2006-08-09 2009-08-03 Conal Tecnologia, S.L IMPROVED SYSTEM FOR THE ELABORATION OF WINES.
EP2201108B1 (en) 2007-10-18 2014-10-08 Danisco US Inc. Enzyme blends for fermentation
WO2009074650A2 (en) 2007-12-12 2009-06-18 Novozymes A/S Brewing process
DE102008008150A1 (en) * 2008-02-08 2009-08-13 Krones Ag Method for the extraction of ethanol from biomass, comprises dry grinding the biomass in a wet malt mill, mashing, fermenting and then distillating the biomass
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
US11999928B2 (en) 2009-11-13 2024-06-04 Novozymes A/S Brewing method
BR112012011106C8 (en) * 2009-11-13 2019-11-26 Novozymes As mashing method
US20110217415A1 (en) * 2010-03-05 2011-09-08 Lurgi, Inc. Hybrid fermentation process
US9175315B2 (en) 2010-06-18 2015-11-03 Butamax Advanced Biofuels Llc Production of alcohol esters and in situ product removal during alcohol fermentation
WO2012084225A1 (en) * 2010-12-22 2012-06-28 Direvo Industrial Biotechnology Gmbh Improving fermentation processes and by-products
EP2654567B1 (en) 2010-12-22 2018-04-04 Novozymes North America, Inc. Process for producing fermentation products from starch containing materials
US20140206058A1 (en) * 2011-03-08 2014-07-24 Poet Research, Inc. Systems and methods for improving stillage
US20130164795A1 (en) * 2011-06-17 2013-06-27 Butamax(Tm) Advanced Biofuels Llc Co-products from biofuel production processes and methods of making
EP2787070A3 (en) * 2011-07-21 2015-03-11 AB Enzymes GmbH Process of lysing yeast cell walls
PL2997144T3 (en) * 2013-05-18 2020-06-29 Direvo Industrial Biotechnology Gmbh Enzyme compositions for the improvement of fermentation processes and by-products
US11939552B2 (en) 2013-06-24 2024-03-26 Novozymes A/S Process of recovering oil
CN116334149A (en) 2013-06-24 2023-06-27 诺维信公司 Process for extracting oil from distilled water
BR102015016382B1 (en) * 2015-07-07 2021-12-07 Margot Stinglwagner MANUFACTURING PROCESS OF DIAMOND FROM DRY CASSAVA FLOUR
CN113999833A (en) * 2021-12-01 2022-02-01 山东天博食品配料有限公司 Culture medium for producing protease by fermenting mucor and application thereof
CN114620831A (en) * 2022-01-26 2022-06-14 四川轻化工大学 Biological promoter and preparation method and application thereof

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2016476A (en) * 1977-11-29 1979-09-26 Walker & Sons Inc Hiram High potency glucamylase and alpha amylase enzyme system by cultivation of aspergillus niger
JPS57159495A (en) * 1981-03-30 1982-10-01 Denki Kagaku Kogyo Kk Preparation of glucose
JPS60232083A (en) * 1984-05-02 1985-11-18 Ueda Kagaku Kogyo Kk Production of unrefined shochu (low-class distilled spirit)
US4952504A (en) * 1987-07-28 1990-08-28 Pavilon Stanley J Method for producing ethanol from biomass
EP0411780A2 (en) * 1989-07-18 1991-02-06 KAMPEN, Willem Hemmo Continuous process for the recovery of betaine
WO1992020777A1 (en) * 1991-05-17 1992-11-26 Solvay Enzymes, Inc. Process for producing ethanol
US5231016A (en) * 1988-05-02 1993-07-27 Rhone-Poulenc Chimie Microbiological production of itaconic acid
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
WO2001062947A1 (en) * 2000-02-23 2001-08-30 Novozymes A/S Fermentation with a phytase
WO2002038787A2 (en) * 2000-11-10 2002-05-16 Novozymes A/S Secondary liquefaction of starch in ethanol production

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2016476A (en) * 1977-11-29 1979-09-26 Walker & Sons Inc Hiram High potency glucamylase and alpha amylase enzyme system by cultivation of aspergillus niger
JPS57159495A (en) * 1981-03-30 1982-10-01 Denki Kagaku Kogyo Kk Preparation of glucose
JPS60232083A (en) * 1984-05-02 1985-11-18 Ueda Kagaku Kogyo Kk Production of unrefined shochu (low-class distilled spirit)
US4952504A (en) * 1987-07-28 1990-08-28 Pavilon Stanley J Method for producing ethanol from biomass
US5231016A (en) * 1988-05-02 1993-07-27 Rhone-Poulenc Chimie Microbiological production of itaconic acid
EP0411780A2 (en) * 1989-07-18 1991-02-06 KAMPEN, Willem Hemmo Continuous process for the recovery of betaine
WO1992020777A1 (en) * 1991-05-17 1992-11-26 Solvay Enzymes, Inc. Process for producing ethanol
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
WO2001062947A1 (en) * 2000-02-23 2001-08-30 Novozymes A/S Fermentation with a phytase
WO2002038787A2 (en) * 2000-11-10 2002-05-16 Novozymes A/S Secondary liquefaction of starch in ethanol production

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 198245, Derwent World Patents Index; Class D17, AN 1982-96166E, XP002902688 *
DATABASE WPI Section Ch Week 198601, Derwent World Patents Index; Class D16, AN 1986-004518, XP002902687 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101545424B1 (en) 2004-05-27 2015-08-18 다니스코 유에스 인크. Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
US9234167B2 (en) 2009-03-03 2016-01-12 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol
US9416376B2 (en) 2009-03-03 2016-08-16 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol

Also Published As

Publication number Publication date
WO2002074895A2 (en) 2002-09-26
AU2002242633A1 (en) 2002-10-03
EP1373539A2 (en) 2004-01-02

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