JPS5446812A - Separation of granulocyte - Google Patents

Separation of granulocyte

Info

Publication number
JPS5446812A
JPS5446812A JP11260077A JP11260077A JPS5446812A JP S5446812 A JPS5446812 A JP S5446812A JP 11260077 A JP11260077 A JP 11260077A JP 11260077 A JP11260077 A JP 11260077A JP S5446812 A JPS5446812 A JP S5446812A
Authority
JP
Japan
Prior art keywords
column
granulocytes
dispersion
hematocyte
erythrocyte
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP11260077A
Other languages
Japanese (ja)
Inventor
Yoshinori Takenaka
Nobuaki Tsuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Chemical Industry Co Ltd
Original Assignee
Asahi Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Industry Co Ltd filed Critical Asahi Chemical Industry Co Ltd
Priority to JP11260077A priority Critical patent/JPS5446812A/en
Publication of JPS5446812A publication Critical patent/JPS5446812A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURPOSE: To separate granulocytes useful for the remedy of granulocytopenia, in high purity and yield, by contacting a hematocyte dispersion with a fibrous material mainly composed of regenerated cellulose, thereby adosorbing the granulocytes thereto.
CONSTITUTION: A hematocyte dispersion containing heparin is passed through a column packed with a fibrous metarial mainly composed of regenerated cellulose, and thereafter, granulocytes selectively adsorbed to the adsorbent is desorbed and eluted by passing a liquid containing plasmaprotein through the column. When a leukocyte dispersion obtained by removing most part of erythrocyte from blood is passed through the column, most part of granulocyte and a part of erythrocyte are adsorbed to the adsorbent, and almost all lymphocytes are discharged from the column thereby obtaining the lymphocytes in high purity and yield.
COPYRIGHT: (C)1979,JPO&Japio
JP11260077A 1977-09-21 1977-09-21 Separation of granulocyte Pending JPS5446812A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11260077A JPS5446812A (en) 1977-09-21 1977-09-21 Separation of granulocyte

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11260077A JPS5446812A (en) 1977-09-21 1977-09-21 Separation of granulocyte

Publications (1)

Publication Number Publication Date
JPS5446812A true JPS5446812A (en) 1979-04-13

Family

ID=14590785

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11260077A Pending JPS5446812A (en) 1977-09-21 1977-09-21 Separation of granulocyte

Country Status (1)

Country Link
JP (1) JPS5446812A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0319961A2 (en) * 1987-12-10 1989-06-14 Japan Immuno Research Laboratories Co., Ltd. Removal method of granulocytes from blood and granulocyte removing apparatus therefor
WO1995007704A1 (en) * 1993-09-15 1995-03-23 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Process for the stabilisation and long-term conservation of red blood cells
WO2006025371A1 (en) 2004-08-30 2006-03-09 Kaneka Corporation Granulocyte adsorbent
WO2006093205A1 (en) * 2005-03-03 2006-09-08 Kaneka Corporation Method and device for cell preparation

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0319961A2 (en) * 1987-12-10 1989-06-14 Japan Immuno Research Laboratories Co., Ltd. Removal method of granulocytes from blood and granulocyte removing apparatus therefor
JPH02193069A (en) * 1987-12-10 1990-07-30 Nippon Koutai Kenkyusho:Kk Method and apparatus for removing granulocyte in blood
US5403719A (en) * 1987-12-10 1995-04-04 Japan Immuno Research Laboratories Co., Ltd. Method for assessing prognosis of a patient undergoing therapy for cancer
WO1995007704A1 (en) * 1993-09-15 1995-03-23 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Process for the stabilisation and long-term conservation of red blood cells
WO2006025371A1 (en) 2004-08-30 2006-03-09 Kaneka Corporation Granulocyte adsorbent
WO2006093205A1 (en) * 2005-03-03 2006-09-08 Kaneka Corporation Method and device for cell preparation

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