JPS5446812A - Separation of granulocyte - Google Patents
Separation of granulocyteInfo
- Publication number
- JPS5446812A JPS5446812A JP11260077A JP11260077A JPS5446812A JP S5446812 A JPS5446812 A JP S5446812A JP 11260077 A JP11260077 A JP 11260077A JP 11260077 A JP11260077 A JP 11260077A JP S5446812 A JPS5446812 A JP S5446812A
- Authority
- JP
- Japan
- Prior art keywords
- column
- granulocytes
- dispersion
- hematocyte
- erythrocyte
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: To separate granulocytes useful for the remedy of granulocytopenia, in high purity and yield, by contacting a hematocyte dispersion with a fibrous material mainly composed of regenerated cellulose, thereby adosorbing the granulocytes thereto.
CONSTITUTION: A hematocyte dispersion containing heparin is passed through a column packed with a fibrous metarial mainly composed of regenerated cellulose, and thereafter, granulocytes selectively adsorbed to the adsorbent is desorbed and eluted by passing a liquid containing plasmaprotein through the column. When a leukocyte dispersion obtained by removing most part of erythrocyte from blood is passed through the column, most part of granulocyte and a part of erythrocyte are adsorbed to the adsorbent, and almost all lymphocytes are discharged from the column thereby obtaining the lymphocytes in high purity and yield.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11260077A JPS5446812A (en) | 1977-09-21 | 1977-09-21 | Separation of granulocyte |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11260077A JPS5446812A (en) | 1977-09-21 | 1977-09-21 | Separation of granulocyte |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5446812A true JPS5446812A (en) | 1979-04-13 |
Family
ID=14590785
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11260077A Pending JPS5446812A (en) | 1977-09-21 | 1977-09-21 | Separation of granulocyte |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5446812A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0319961A2 (en) * | 1987-12-10 | 1989-06-14 | Japan Immuno Research Laboratories Co., Ltd. | Removal method of granulocytes from blood and granulocyte removing apparatus therefor |
WO1995007704A1 (en) * | 1993-09-15 | 1995-03-23 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Process for the stabilisation and long-term conservation of red blood cells |
WO2006025371A1 (en) | 2004-08-30 | 2006-03-09 | Kaneka Corporation | Granulocyte adsorbent |
WO2006093205A1 (en) * | 2005-03-03 | 2006-09-08 | Kaneka Corporation | Method and device for cell preparation |
-
1977
- 1977-09-21 JP JP11260077A patent/JPS5446812A/en active Pending
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0319961A2 (en) * | 1987-12-10 | 1989-06-14 | Japan Immuno Research Laboratories Co., Ltd. | Removal method of granulocytes from blood and granulocyte removing apparatus therefor |
JPH02193069A (en) * | 1987-12-10 | 1990-07-30 | Nippon Koutai Kenkyusho:Kk | Method and apparatus for removing granulocyte in blood |
US5403719A (en) * | 1987-12-10 | 1995-04-04 | Japan Immuno Research Laboratories Co., Ltd. | Method for assessing prognosis of a patient undergoing therapy for cancer |
WO1995007704A1 (en) * | 1993-09-15 | 1995-03-23 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Process for the stabilisation and long-term conservation of red blood cells |
WO2006025371A1 (en) | 2004-08-30 | 2006-03-09 | Kaneka Corporation | Granulocyte adsorbent |
WO2006093205A1 (en) * | 2005-03-03 | 2006-09-08 | Kaneka Corporation | Method and device for cell preparation |
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