JPH11502820A - 有機シアン化試薬により活性化された可溶性炭水化物を使用する免疫原性構築物の製造 - Google Patents
有機シアン化試薬により活性化された可溶性炭水化物を使用する免疫原性構築物の製造Info
- Publication number
- JPH11502820A JPH11502820A JP8528653A JP52865396A JPH11502820A JP H11502820 A JPH11502820 A JP H11502820A JP 8528653 A JP8528653 A JP 8528653A JP 52865396 A JP52865396 A JP 52865396A JP H11502820 A JPH11502820 A JP H11502820A
- Authority
- JP
- Japan
- Prior art keywords
- polysaccharide
- cdap
- component
- dextran
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
- A61K47/646—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent the entire peptide or protein drug conjugate elicits an immune response, e.g. conjugate vaccines
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
- A61K2039/6031—Proteins
- A61K2039/6037—Bacterial toxins, e.g. diphteria toxoid [DT], tetanus toxoid [TT]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/62—Medicinal preparations containing antigens or antibodies characterised by the link between antigen and carrier
- A61K2039/627—Medicinal preparations containing antigens or antibodies characterised by the link between antigen and carrier characterised by the linker
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.工程: (a)少なくとも1つの第1炭水化物含有成分を有機シアン化試薬で活性化し て活性化された炭水化物を形成し、そして (b)前記活性化された炭水化物を第2成分に直接的または間接的にカップリ ングして、免疫応答を刺激することができる免疫原性構築物を形成する、 を含んでなる免疫原性構築物を製造する方法。 2.前記有機シアン化試薬が1−シアノ−4−(ジメチルアミノ)−ピリジニ ウムテトラフルオロボレート、N−シアノトリエチル−アンモニウムテトラフル オロボレート、またはp−ニトロフェニルシアネートである、請求項1に記載の 方法。 3.前記有機シアン化試薬が1−シアノ−4−(ジメチルアミノ)−ピリジニ ウムテトラフルオロボレートである、請求項1に記載の方法。 4.前記第1成分および第2成分が水溶性である、請求項3に記載の方法。 5.前記活性化工程(a)を8〜10のpHにおいて実施し、そして前記カッ プリング工程(b)を7〜9のpHにおいて実施する、請求項3に記載の方法。 6.前記活性化工程(a)をトリエチルアミンの存在において実施する、請求 項3に記載の方法。 7.第1成分を2官能性またはヘテロ官能性のスペーサー試薬に共有結合的に 結合し、かつ第2成分をスペーサー試薬に共有結合的に結合することによって工 程(b)を間接的に実施する、請求項1に記載の方法。 8.前記スペーサー試薬がエチレンジアミン、1,6−ヘキサンジアミン、ア ジピン酸ジヒドラジド、シスタミン、グリシン、およびリジンから成る群より選 択される、請求項7に記載の方法。 9.第1成分が多糖であり、そして第2成分がタンパク質である、請求項1に 記載の方法。 10.多糖がデキストラン、肺炎球菌(Pneumococcal)多糖、イン フルエンザ菌(Haemophilus influenzae)多糖、グルー プAストレプトコッカス(streptococcus)多糖、グループBスト レプトコッカス(streptococcus)多糖、および髄膜炎菌(N.m eningitidis)多糖から成る群より選択される、請求項9に記載の方 法。 11.第1成分が水溶性のウイルスまたは細菌の多糖である、請求項1に記載の 方法。 12.第2成分が水溶性タンパク質である、請求項1に記載の方法。 13.第2成分がウシ血清アルブミン、百日咳トキソイド、破傷風トキソイド、 マラリア誘導ペプチド、抗体、トキソイド、およびリポタンパク質から成る群よ り選択される、請求項1に記載の方法。 14.免疫原性構築物がPT−Pn、PT−PRP、TT−Pn、抗体−デキス トラン、およびペプチド−TT−Pnから成る群より選択される複合体である、 請求項1に記載の方法。 15.十分に低いpKaを有する求核物質を形成するように第2成分を誘導化す る、請求項1に記載の方法。 16.前記求核物質がヒドラジドである、請求項15に記載の方法。 17.前記カップリング工程(b)を約8より低いpHにおいて実 施する、請求項16に記載の方法。 18.十分に低いpKaを有する求核物質を形成するように第2成分を誘導化す る、請求項7に記載の方法。 19.前記求核物質がヒドラジドである、請求項18に記載の方法。 20.前記カップリング工程(b)を約8より低いpHにおいて実施する、請求 項19に記載の方法。 21.(a)(i)少なくとも1つの第1炭水化物含有成分を有機シアン化試薬 で活性化し、そして(ii)前記活性化された炭水化物を第2成分に共有結合的 に結合することを包含する工程により免疫原性構築物を調製し、そして (b)前記免疫原性構築物を患者に投与する、 ことを含んでなる、免疫応答を発生させる方法。 22.前記有機シアン化試薬が1−シアノ−4−(ジメチルアミノ)−ピリジニ ウムテトラフルオロボレートである、請求項21に記載の方法。 23.前記活性化工程(a)をトリエチルアミンの存在において実施する、請求 項22に記載の方法。 24.第1成分が多糖であり、そして第2成分が水溶性タンパク質である、請求 項22に記載の方法。 25.多糖がデキストラン、肺炎球菌(Pneumococcal)多糖、イン フルエンザ菌(Haemophilus influenzae)多糖、グルー プAストレプトコッカス(streptococcus)多糖、グループBスト レプトコッカス(streptococcus)多糖、および髄膜炎菌(N.m eningitidis)多糖から成る群より選択される、請求項24に記載の方 法。 26.第2成分がウシ血清アルブミン、百日咳トキソイド、破傷風トキソイド、 マラリア誘導ペプチド、抗体、トキソイド、およびリポタンパク質から成る群よ り選択される、請求項22に記載の方法。 27.免疫原性構築物がPT−Pn、PT−PRP、TT−Pn、抗体−デキス トラン、およびペプチド−TT−Pnから成る群より選択される複合体である、 請求項22に記載の方法。 28.十分に低いpKaを有する求核物質を形成するように第2成分を誘導化す る、請求項21に記載の方法。 29.前記求核物質がヒドラジドである、請求項28に記載の方法。 30.前記カップリング工程(b)を約8より低いpHにおいて実施する、請求 項29記載の方法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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US08/408,717 US5651971A (en) | 1993-09-22 | 1995-03-22 | Producing immunogenic constructs using soluble carbohydrates activated via organic cyanylating reagents |
US08/408,717 | 1995-03-22 | ||
US48266195A | 1995-06-07 | 1995-06-07 | |
US08/482,661 | 1995-06-07 | ||
PCT/US1996/004013 WO1996029094A1 (en) | 1995-03-22 | 1996-03-22 | Producing immunogenic constructs using soluble carbohydrates activated via organic cyanylating reagents |
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JP2007331707A Division JP2008101022A (ja) | 1995-03-22 | 2007-12-25 | 有機シアン化試薬により活性化された可溶性炭水化物を使用する免疫原性構築物の製造 |
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JP8528653A Pending JPH11502820A (ja) | 1995-03-22 | 1996-03-22 | 有機シアン化試薬により活性化された可溶性炭水化物を使用する免疫原性構築物の製造 |
JP2007331707A Withdrawn JP2008101022A (ja) | 1995-03-22 | 2007-12-25 | 有機シアン化試薬により活性化された可溶性炭水化物を使用する免疫原性構築物の製造 |
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JP2007331707A Withdrawn JP2008101022A (ja) | 1995-03-22 | 2007-12-25 | 有機シアン化試薬により活性化された可溶性炭水化物を使用する免疫原性構築物の製造 |
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EP (1) | EP0814833B1 (ja) |
JP (2) | JPH11502820A (ja) |
AT (1) | ATE241384T1 (ja) |
AU (1) | AU712981B2 (ja) |
CA (1) | CA2215933C (ja) |
DE (1) | DE69628418T2 (ja) |
ES (1) | ES2200059T3 (ja) |
NZ (1) | NZ304715A (ja) |
WO (1) | WO1996029094A1 (ja) |
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DE3541186A1 (de) * | 1985-11-21 | 1987-05-27 | Boehringer Mannheim Gmbh | Wasserloesliche, stabilisierte peroxidasederivate, verfahren zu ihrer herstellung und verwendung zur bestimmung von wasserstoffperoxid |
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- 1996-03-22 AT AT96908900T patent/ATE241384T1/de not_active IP Right Cessation
- 1996-03-22 WO PCT/US1996/004013 patent/WO1996029094A1/en active IP Right Grant
- 1996-03-22 DE DE69628418T patent/DE69628418T2/de not_active Expired - Lifetime
- 1996-03-22 EP EP96908900A patent/EP0814833B1/en not_active Expired - Lifetime
- 1996-03-22 CA CA002215933A patent/CA2215933C/en not_active Expired - Lifetime
- 1996-03-22 JP JP8528653A patent/JPH11502820A/ja active Pending
- 1996-03-22 ES ES96908900T patent/ES2200059T3/es not_active Expired - Lifetime
- 1996-03-22 AU AU52591/96A patent/AU712981B2/en not_active Expired
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JP2009520761A (ja) * | 2005-12-22 | 2009-05-28 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | ワクチン |
JP2012211165A (ja) * | 2005-12-22 | 2012-11-01 | Glaxosmithkline Biologicals Sa | ワクチン |
JP2015155417A (ja) * | 2009-12-17 | 2015-08-27 | フィナ バイオソリューションズ リミテッド ライアビリティ カンパニー | コンジュゲートワクチンの製造における多糖の活性化のための化学試薬 |
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Also Published As
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CA2215933C (en) | 2009-10-13 |
ES2200059T3 (es) | 2004-03-01 |
JP2008101022A (ja) | 2008-05-01 |
AU5259196A (en) | 1996-10-08 |
EP0814833A1 (en) | 1998-01-07 |
EP0814833B1 (en) | 2003-05-28 |
NZ304715A (en) | 1999-07-29 |
WO1996029094A1 (en) | 1996-09-26 |
DE69628418T2 (de) | 2004-04-01 |
DE69628418D1 (de) | 2003-07-03 |
AU712981B2 (en) | 1999-11-18 |
CA2215933A1 (en) | 1996-09-26 |
ATE241384T1 (de) | 2003-06-15 |
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