JPH07503622A - 多価の一本鎖抗体 - Google Patents
多価の一本鎖抗体Info
- Publication number
- JPH07503622A JPH07503622A JP6514437A JP51443794A JPH07503622A JP H07503622 A JPH07503622 A JP H07503622A JP 6514437 A JP6514437 A JP 6514437A JP 51443794 A JP51443794 A JP 51443794A JP H07503622 A JPH07503622 A JP H07503622A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- sequence
- chain antibody
- fragment
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/42—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins
- C07K16/4208—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig
- C07K16/4241—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-human or anti-animal Ig
- C07K16/4258—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-human or anti-animal Ig against anti-receptor Ig
- C07K16/4266—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-human or anti-animal Ig against anti-receptor Ig against anti-tumor receptor Ig
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (6)
- 1.2以上の一本鎖抗体フラグメントを含んで成り、各フラグメントが抗原に対 する親和性を存しており、ここでそのフラグメントは第一のペプチドリンカーに より共有結合されており、そして各フラグメントは: (a)軽鎖可変ドメインを含んで成る第一ポリペプチド;(b)重鎖可変ドメイ ンを含んで成る第二ポリペプチド;及び(c)この第一と第二のポリペプチドを 機花的な結合性成分へと連結せしめる第二のペプチドリンカー;を含んで成る、 多価の一本鎖抗体。
- 2.この第一のペプチドリンカーが下記のアミノ酸配列【配列があります】 を有する、請求項1記載の多価の一本鎖抗体。
- 3.この軽鎖可変領域が、図3に示すものと実質的に同じアミノ酸配列を有して おり、そしてこの重鎖可変領域が、図5に示すものと実質的に同じアミノ酸配列 を有している、請求項1記載の多価の一本鎖抗体。
- 4.この第一と第二のペプチドリンカーが実質的に同じアミノ酸配列を有する、 請求項1記載の多価の一本鎖抗体。
- 5.多価の一本鎖抗体をコードするDNA配列であって、この多価の一本鎖抗体 が2以上の一本鎖抗体フラグメントを含んで成り、各フラグメントが抗原に対す る親和性を有しており、ここでそれらのフラグメントは第一のペプチドリンカー により共有結合されており、そして各フラグメントは; (a)軽鎖可変ドメインを含んで成る第一ポリペプチド;(b)重鎖可変ドメイ ンを含んで成る第二ポリペプチド;及び(c)この第一と第二のポリペプチドを 機能的な結合性成分へと連結せしめる第二のペプチドリンカー;を含んで成る、 DNA配列。
- 6.この第一のポリペプチドをコードする配列が図2のそれと実質的に同じであ り、そして第二のポリペプチドをコードする配列が図3のそれと実質的に同じで ある、請求項5記載のDNA配列。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US99026392A | 1992-12-11 | 1992-12-11 | |
US990,263 | 1992-12-11 | ||
PCT/US1993/012039 WO1994013806A1 (en) | 1992-12-11 | 1993-12-10 | Multivalent single chain antibodies |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH07503622A true JPH07503622A (ja) | 1995-04-20 |
JP3312357B2 JP3312357B2 (ja) | 2002-08-05 |
Family
ID=25535963
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51443794A Expired - Lifetime JP3312357B2 (ja) | 1992-12-11 | 1993-12-10 | 多価の一本鎖抗体 |
Country Status (9)
Country | Link |
---|---|
US (2) | US5877291A (ja) |
EP (1) | EP0628078B1 (ja) |
JP (1) | JP3312357B2 (ja) |
AT (1) | ATE187494T1 (ja) |
CA (1) | CA2117477C (ja) |
DE (1) | DE69327229T2 (ja) |
HK (1) | HK1019014A1 (ja) |
SG (1) | SG55079A1 (ja) |
WO (1) | WO1994013806A1 (ja) |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997023639A1 (fr) * | 1995-12-22 | 1997-07-03 | Toray Industries, Inc. | Procede de production de proteines fusionnees biologiquement actives |
WO2005056603A1 (ja) * | 2003-12-12 | 2005-06-23 | Chugai Seiyaku Kabushiki Kaisha | 細胞死誘導剤 |
US7691588B2 (en) | 2003-03-13 | 2010-04-06 | Chugai Seiyaku Kabushiki Kaisha | Ligand having agonistic activity to mutated receptor |
US7696325B2 (en) | 1999-03-10 | 2010-04-13 | Chugai Seiyaku Kabushiki Kaisha | Polypeptide inducing apoptosis |
JP2011506968A (ja) * | 2007-12-13 | 2011-03-03 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | エレクトロブロッティングのためのイオンリザーバーとしてのポリマー収着シート |
US7993642B2 (en) | 2003-12-12 | 2011-08-09 | Chugai Seiyaku Kabushiki Kaisha | Anti-MPL antibodies |
US8034903B2 (en) | 2000-10-20 | 2011-10-11 | Chugai Seiyaku Kabushiki Kaisha | Degraded TPO agonist antibody |
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ES2165851T3 (es) * | 1991-11-25 | 2002-04-01 | Enzon Inc | Proteinas multivalentes que se unen a antigenos. |
-
1993
- 1993-12-10 SG SG1996005043A patent/SG55079A1/en unknown
- 1993-12-10 DE DE69327229T patent/DE69327229T2/de not_active Expired - Lifetime
- 1993-12-10 AT AT94903587T patent/ATE187494T1/de not_active IP Right Cessation
- 1993-12-10 JP JP51443794A patent/JP3312357B2/ja not_active Expired - Lifetime
- 1993-12-10 CA CA002117477A patent/CA2117477C/en not_active Expired - Fee Related
- 1993-12-10 WO PCT/US1993/012039 patent/WO1994013806A1/en active IP Right Grant
- 1993-12-10 EP EP94903587A patent/EP0628078B1/en not_active Expired - Lifetime
-
1994
- 1994-06-21 US US08/263,911 patent/US5877291A/en not_active Expired - Lifetime
-
1995
- 1995-06-07 US US08/481,006 patent/US5892020A/en not_active Expired - Lifetime
-
1998
- 1998-12-28 HK HK98115758A patent/HK1019014A1/xx not_active IP Right Cessation
Cited By (23)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1997023639A1 (fr) * | 1995-12-22 | 1997-07-03 | Toray Industries, Inc. | Procede de production de proteines fusionnees biologiquement actives |
US7696325B2 (en) | 1999-03-10 | 2010-04-13 | Chugai Seiyaku Kabushiki Kaisha | Polypeptide inducing apoptosis |
US8586039B2 (en) | 2000-10-20 | 2013-11-19 | Chugai Seiyaku Kabushiki Kaisha | Degraded TPO agonist antibody |
US8034903B2 (en) | 2000-10-20 | 2011-10-11 | Chugai Seiyaku Kabushiki Kaisha | Degraded TPO agonist antibody |
US8158385B2 (en) | 2002-10-11 | 2012-04-17 | Chugai Seiyaku Kabushiki Kaisha | Cell death-inducing agent |
US7691588B2 (en) | 2003-03-13 | 2010-04-06 | Chugai Seiyaku Kabushiki Kaisha | Ligand having agonistic activity to mutated receptor |
WO2005056603A1 (ja) * | 2003-12-12 | 2005-06-23 | Chugai Seiyaku Kabushiki Kaisha | 細胞死誘導剤 |
JPWO2005056603A1 (ja) * | 2003-12-12 | 2009-05-28 | 中外製薬株式会社 | 細胞死誘導剤 |
US7993642B2 (en) | 2003-12-12 | 2011-08-09 | Chugai Seiyaku Kabushiki Kaisha | Anti-MPL antibodies |
US8008073B2 (en) | 2003-12-12 | 2011-08-30 | Chugai Seiyaku Kabushiki Kaisha | Anti-Mpl antibodies |
JP4767016B2 (ja) * | 2003-12-12 | 2011-09-07 | 中外製薬株式会社 | 細胞死誘導剤 |
US9493569B2 (en) | 2005-03-31 | 2016-11-15 | Chugai Seiyaku Kabushiki Kaisha | Structural isomers of sc(Fv)2 |
US10011858B2 (en) | 2005-03-31 | 2018-07-03 | Chugai Seiyaku Kabushiki Kaisha | Methods for producing polypeptides by regulating polypeptide association |
US11168344B2 (en) | 2005-03-31 | 2021-11-09 | Chugai Seiyaku Kabushiki Kaisha | Methods for producing polypeptides by regulating polypeptide association |
US8945543B2 (en) | 2005-06-10 | 2015-02-03 | Chugai Seiyaku Kabushiki Kaisha | Stabilizer for protein preparation comprising meglumine and use thereof |
US9241994B2 (en) | 2005-06-10 | 2016-01-26 | Chugai Seiyaku Kabushiki Kaisha | Pharmaceutical compositions containing sc(Fv)2 |
US9777066B2 (en) | 2005-06-10 | 2017-10-03 | Chugai Seiyaku Kabushiki Kaisha | Pharmaceutical compositions containing sc(Fv)2 |
US9670269B2 (en) | 2006-03-31 | 2017-06-06 | Chugai Seiyaku Kabushiki Kaisha | Methods of modifying antibodies for purification of bispecific antibodies |
US10934344B2 (en) | 2006-03-31 | 2021-03-02 | Chugai Seiyaku Kabushiki Kaisha | Methods of modifying antibodies for purification of bispecific antibodies |
JP2011506968A (ja) * | 2007-12-13 | 2011-03-03 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | エレクトロブロッティングのためのイオンリザーバーとしてのポリマー収着シート |
JP2015531751A (ja) * | 2012-07-23 | 2015-11-05 | ザイムワークス,インコーポレイテッド | 選択的対である軽鎖および重鎖を含む免疫グロブリン構築物 |
US11124576B2 (en) | 2013-09-27 | 2021-09-21 | Chungai Seiyaku Kabushiki Kaisha | Method for producing polypeptide heteromultimer |
US11649262B2 (en) | 2015-12-28 | 2023-05-16 | Chugai Seiyaku Kabushiki Kaisha | Method for promoting efficiency of purification of Fc region-containing polypeptide |
Also Published As
Publication number | Publication date |
---|---|
AU680461B2 (en) | 1997-07-31 |
AU5747794A (en) | 1994-07-04 |
WO1994013806A1 (en) | 1994-06-23 |
CA2117477C (en) | 2001-06-12 |
DE69327229D1 (de) | 2000-01-13 |
ATE187494T1 (de) | 1999-12-15 |
US5877291A (en) | 1999-03-02 |
JP3312357B2 (ja) | 2002-08-05 |
EP0628078B1 (en) | 1999-12-08 |
SG55079A1 (en) | 1998-12-21 |
CA2117477A1 (en) | 1994-06-23 |
US5892020A (en) | 1999-04-06 |
HK1019014A1 (en) | 2000-01-14 |
EP0628078A1 (en) | 1994-12-14 |
DE69327229T2 (de) | 2000-03-30 |
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