JPH06256152A - Skin cosmetic - Google Patents
Skin cosmeticInfo
- Publication number
- JPH06256152A JPH06256152A JP6252193A JP6252193A JPH06256152A JP H06256152 A JPH06256152 A JP H06256152A JP 6252193 A JP6252193 A JP 6252193A JP 6252193 A JP6252193 A JP 6252193A JP H06256152 A JPH06256152 A JP H06256152A
- Authority
- JP
- Japan
- Prior art keywords
- skin
- glabridin
- active oxygen
- formulation
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Cosmetics (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、紫外線等の外的刺激で
生じる炎症や皮膚メラニンの生成もしくは沈着を抑制
し、紅斑(日焼け)、皮膚黒化、シミ、ソバカス等を防
止することができる皮膚化粧料に関するものである。INDUSTRIAL APPLICABILITY The present invention can suppress inflammation caused by external stimuli such as ultraviolet rays and the production or deposition of skin melanin, and prevent erythema (sunburn), skin blackening, spots, freckles and the like. It relates to skin cosmetics.
【0002】[0002]
【従来の技術】皮膚の色が黒くなる過程の最終段階にお
いては、アミノ酸の一種であるチロシンがチロシナーゼ
の作用を受けることによって黒色色素・メラニンを生成
する。そこで、チロシナーゼの働きを阻害する作用を有
する種々の薬剤、たとえばグルタチオン、ビタミンCま
たはその誘導体等を皮膚に塗布することにより、色素の
沈着を防止しようとする試みが従来なされてきた。しか
しながら、これらの薬剤は局所適用における安定性や有
効性の点で、必ずしも満足できるものではない。2. Description of the Related Art In the final stage of the process of darkening the skin color, tyrosine, which is one of the amino acids, produces the black pigment melanin by the action of tyrosinase. Therefore, it has been conventionally attempted to prevent pigment deposition by applying various agents having an action of inhibiting the action of tyrosinase, such as glutathione, vitamin C or its derivatives, to the skin. However, these drugs are not always satisfactory in terms of stability and effectiveness in topical application.
【0003】これは、チロシナーゼ以外にもメラニン生
成の原因や引き金となるもの、たとえば紫外線によるメ
ラノサイトの活性化や炎症による種々のケミカルメディ
エーターの関与、紫外線により生成する活性酸素類の関
与、更には皮脂過酸化物等の関与が報告されているよう
に、単にチロシナーゼの働きを阻害するだけの薬剤を塗
布してもメラニンの沈着を十分防止することは困難なた
めである。In addition to tyrosinase, this is a cause and trigger of melanin production, for example, activation of melanocytes by ultraviolet rays, involvement of various chemical mediators due to inflammation, involvement of active oxygen species produced by ultraviolet rays, and sebum. This is because it has been difficult to sufficiently prevent the deposition of melanin even by applying a drug that merely inhibits the action of tyrosinase, as reported by the involvement of peroxides and the like.
【0004】[0004]
【発明が解決しようとする課題】本発明の目的は、上述
のように多くの要因が関与している皮膚黒色化を効果的
に防止することができ、しかも安全性の点でも優れてい
る、新しい皮膚化粧料を提供することにある。DISCLOSURE OF THE INVENTION The object of the present invention is to effectively prevent skin blackening, which is associated with many factors as described above, and is also excellent in safety. To provide new skin cosmetics.
【0005】[0005]
【課題を解決するための手段】本発明による皮膚化粧料
は、必須の有効成分としてグラブリジンおよび活性酸素
消去剤を含有することを特徴とし、これら必須成分の相
乗作用により、紫外線等の外的刺激で生じる炎症や皮膚
メラニンの生成・沈着を抑制するものである。本発明の
皮膚化粧料を構成する有効成分の一つであるグラブリジ
ンは下記化1の構造式を有する化合物であって、天然に
は、甘草の一種である Glycyrrhizaglabra Linne var.
(通称ロシア・アフガン・トルコカンゾウ)に微量含ま
れている。The skin cosmetic according to the present invention is characterized in that it contains glabridin and an active oxygen scavenger as essential active ingredients, and the synergistic action of these essential ingredients causes external stimuli such as ultraviolet rays. It suppresses inflammation and skin melanin production and deposition. Glabridin, which is one of the active ingredients constituting the skin cosmetic of the present invention, is a compound having a structural formula of the following chemical formula 1, and is naturally a kind of licorice, Glycyrrhizaglabra Linne var.
It is contained in a very small amount (commonly known as Russia, Afghan and Turkish liquorice).
【0006】[0006]
【化1】 [Chemical 1]
【0007】グラブリジンについては、抗菌作用、抗酸
化作用、抗う蝕作用、抗プラスミン作用等の薬理作用を
有することが確認されており、さらに、メラニン生成抑
制作用を有することも知られている(特開平1−311
011号公報)。しかしながら、従来確認されていたグ
ラブリジンのメラニン生成抑制作用はグラブリジンを単
用した場合のものであって、それを活性酸素消去剤その
他の生理活性物質と併用した場合の効果は知られていな
かった。It has been confirmed that glabridin has a pharmacological action such as an antibacterial action, an antioxidative action, an anticarious action and an antiplasmin action, and is also known to have a melanin production inhibiting action (special feature). Kaihei 1-311
011). However, the previously confirmed melanin production-inhibitory action of glabridine is only when glabridine is used alone, and the effect of using it in combination with an active oxygen scavenger and other physiologically active substances has not been known.
【0008】グラブリジンを甘草から抽出して本発明の
化粧料製造原料とする場合は、まず甘草の根部またはそ
の水抽出残渣(たとえばグリチルリチンを抽出した残
渣)を有機溶媒で抽出する。抽出溶媒としては、メタノ
ール、エタノール等の低級脂肪族アルコール;アセトン
等の低級脂肪族ケトン;ジオキサン、エチルエーテル等
のエーテル類;塩化メチレン、クロロホルム等のハロゲ
ン化炭化水素類;酢酸エチル、酢酸プロピル、酢酸ブチ
ル等のエステル類;ヘキサン、ベンゼン等の炭化水素
類;およびこれらの有機溶媒の2種以上の混合物を使用
することができる。抽出処理する甘草は、約5〜15倍
量の上記溶媒に浸漬し、常温で静置するか還流下に加熱
する。抽出液から溶媒を留去して得られる抽出物は、通
常5〜10%程度のグラブリジンを含有しており、その
まま本発明の化粧料に使用することもできるが、精製し
て純度を高めたものを用いることにより、より使用効果
に優れ且つ着色も少ない化粧料を得ることができる。When glabridin is extracted from licorice to be used as a raw material for producing cosmetics of the present invention, first, the root portion of licorice or its water extraction residue (for example, the residue obtained by extracting glycyrrhizin) is extracted with an organic solvent. As the extraction solvent, lower aliphatic alcohols such as methanol and ethanol; lower aliphatic ketones such as acetone; ethers such as dioxane and ethyl ether; halogenated hydrocarbons such as methylene chloride and chloroform; ethyl acetate, propyl acetate, etc. Esters such as butyl acetate; hydrocarbons such as hexane and benzene; and mixtures of two or more of these organic solvents can be used. The licorice to be extracted is immersed in about 5 to 15 times the amount of the above solvent, and allowed to stand at room temperature or heated under reflux. The extract obtained by distilling off the solvent from the extract usually contains about 5 to 10% of glabridin and can be used as it is in the cosmetic of the present invention, but it was purified to improve its purity. By using the ones, it is possible to obtain a cosmetic material which is more excellent in use effect and less colored.
【0009】抽出物の精製は、たとえば順相シリカゲル
クロマトグラフィーおよび逆相クロマトグラフィーによ
り処理したのちアセトンから結晶化させる方法により行
うことができ、この方法によれば、比較的容易にグラブ
リジンの純品を得ることができる。精製は、ほかにも合
成吸着体によるカラムクロマトグラフィーや液−液向流
抽出等、任意の有機化合物精製手段を採用して行うこと
ができる。Purification of the extract can be carried out by, for example, a method in which normal phase silica gel chromatography and reverse phase chromatography are carried out, followed by crystallization from acetone. According to this method, pure glabridin is relatively easily prepared. Can be obtained. In addition, the purification can be performed by employing any organic compound purification means such as column chromatography using a synthetic adsorbent or liquid-liquid countercurrent extraction.
【0010】グラブリジンと共に本発明の皮膚化粧料に
含有させる活性酸素消去剤の好ましい具体例としては、
スーパーオキサイドディスムターゼ、カタラーゼ、β−
カロチン、バイカリン、バイカレイン、メリッサ抽出
物、エンメイソウ抽出物、セージ抽出物、ローズマリー
抽出物、エレウテロコック抽出物、イチョウ抽出物、チ
ョウジ抽出物、クジン抽出物、ハマメリス抽出物、ソウ
ハクヒ抽出物、バジル抽出物、オタネニンジン抽出物、
トウキ抽出物、センキュウ抽出物等、活性酸素消去作用
のある酵素、植物抽出物等がある。Preferred specific examples of the active oxygen scavenger contained in the skin cosmetic of the present invention together with glabridin include:
Superoxide dismutase, catalase, β-
Carotene, baicalin, baicalein, melissa extract, nephew extract, sage extract, rosemary extract, eleuterococcus extract, ginkgo extract, clove extract, kujin extract, hamamelis extract, sowakuhi extract, basil extract , Panax ginseng extract,
There are enzymes such as Touki extract, Senkyu extract and the like, active oxygen scavenging enzymes, and plant extracts.
【0011】これらの活性酸素消去剤は、2種以上を本
発明の化粧料に含有させてもよい。皮膚においては、酸
素の存在下に紫外線を受けたとき活性酸素が発生する。
生じた活性酸素は、通常、生体内のスーパーオキサイド
ディスムターゼ、カタラーゼ等の酵素により消去される
が、老化、損傷等により機能が低下した皮膚では活性酸
素が完全には消去されず、残った活性酸素がコラーゲン
の変性、ヒアルロン酸の分解、脂質の過酸化等をひき起
こす。それにより、皮膚はさらなる機能低下を起こす。
また、活性酸素によって生じた過酸化脂質が炎症因子亢
進の原因となり、炎症によるメラニン生成系を誘導す
る。さらに、活性酸素種によっては、チロシンから生じ
るメラニン生成サイクルに直接関与してメラニン生成を
促進することが知られている。本発明で用いる活性酸素
消去剤類は、活性酸素がひき起こす上述の好ましくない
生体反応を防止することが知られており、この作用がグ
ラブリジンの作用と相乗的に作用して、好ましい皮膚の
維持・形成に役立つと共に皮膚の黒色化防止をも可能に
する。Two or more of these active oxygen scavengers may be contained in the cosmetic of the present invention. In the skin, active oxygen is generated when it receives ultraviolet rays in the presence of oxygen.
The generated active oxygen is normally erased by enzymes such as superoxide dismutase and catalase in the body, but the active oxygen is not completely erased in the skin whose function is deteriorated due to aging, damage, etc. Causes denaturation of collagen, decomposition of hyaluronic acid, and peroxidation of lipids. Thereby, the skin further deteriorates.
In addition, lipid peroxide generated by active oxygen causes an increase in inflammatory factors and induces a melanin production system due to inflammation. Furthermore, it is known that some reactive oxygen species directly participate in the melanogenesis cycle generated from tyrosine and promote melanogenesis. The active oxygen scavengers used in the present invention are known to prevent the above-mentioned undesired biological reactions caused by active oxygen, and this action acts synergistically with the action of glabridin to maintain favorable skin. -Helps to form and also prevents blackening of the skin.
【0012】本発明の皮膚化粧料におけるグラブリジン
の好適配合量は、化粧料の種類によっても異なるが、通
常約0.001〜10重量%であり、特に好ましくは約
0.01〜1.0重量%である。また、活性酸素消去剤の
好適配合量は約0.01〜10重量%であり、特に好ま
しくは約0.05〜5重量%であるが、さらに、グラブ
リジンに対して約10〜2000重量%になるように配
合することが望ましい。The preferred amount of glabridin in the skin cosmetic of the present invention varies depending on the kind of the cosmetic, but it is usually about 0.001 to 10% by weight, particularly preferably about 0.01 to 1.0% by weight. %. Further, the preferable blending amount of the active oxygen scavenger is about 0.01 to 10% by weight, particularly preferably about 0.05 to 5% by weight, and further about 10 to 2000% by weight with respect to glabridin. It is desirable to mix them as follows.
【0013】本発明の化粧料は、グラブリジンと活性酸
素消去剤の併用によるメラニン生成抑制作用を有利に利
用することのできる任意の化粧料、たとえば化粧水、乳
液、クリーム、パック、石鹸、ボディーシャンプー等の
形態をとることができる。グラブリジンと活性酸素消去
剤以外の化粧料構成成分、たとえば油脂類、界面活性
剤、増粘剤、色素、香料、防腐剤、エタノール、多価ア
ルコール等は、その化粧料の種類に応じて、必須2成分
の作用を損なわない範囲で任意に選択することができ
る。The cosmetic of the present invention is any cosmetic that can advantageously utilize the melanin production inhibitory action by the combined use of glabridin and an active oxygen scavenger, such as lotion, emulsion, cream, pack, soap, body shampoo. And the like. Cosmetic ingredients other than glabridine and active oxygen scavengers, such as oils and fats, surfactants, thickeners, pigments, fragrances, preservatives, ethanol, polyhydric alcohols, etc., are essential depending on the type of the cosmetic. It can be arbitrarily selected within a range not impairing the action of the two components.
【0014】[0014]
〔グラブリジン製造例〕甘草の根部の細切物500gを
5リットルの酢酸エチルに浸漬し、還流下に2時間加熱
して酢酸エチル可溶成分を抽出した。抽出液を分離した
抽出残渣について同様の操作を繰り返し、合計9リット
ルの抽出液を得た。抽出液の溶媒を減圧下に留去し、グ
ラブリジンを含有する抽出物13.1gを得た。次いで
抽出物をクロロホルムに溶解し、シリカゲル(ワコーゲ
ルC-300,和光純薬工業株式会社製品)にまぶしたのち
乾燥した。この乾燥物を、あらかじめシリカゲル1kgを
充填したカラム上に積層充填し、クロロホルム/メタノ
ール混合液(30:1)で溶出し、グラブリジン含有画分
を採取した。この画分の溶媒を減圧下に留去して固形物
5.8gを得たのち、少量のメタノールに溶解し、逆相
シリカゲル(ODSG-3,水戸化学技術研究所製品)にまぶ
して乾燥し、あらかじめ逆相シリカゲル800gを充填
したカラム上に積層充填した。このカラムに、溶出溶媒
として水−アセトニトリル混合液(30:70)を流し、グ
ラブリジン含有画分を採取した。この画分から溶媒を減
圧下に留去し、得られた固形物(4.3g)をアセトン
40mlに溶解し、5℃で3日間静置して、グラブリジン
の結晶3.8gを得た。以下の各実施例においては、グ
ラブリジンとして上記精製グラブリジンの結晶を用い
た。[Preparation example of glabridin] 500 g of licorice root shredded pieces were immersed in 5 liters of ethyl acetate and heated under reflux for 2 hours to extract ethyl acetate-soluble components. The same operation was repeated for the extraction residue obtained by separating the extract, and a total of 9 liters of extract was obtained. The solvent of the extract was distilled off under reduced pressure to obtain 13.1 g of an extract containing glabridin. Next, the extract was dissolved in chloroform, sprinkled on silica gel (Wakogel C-300, product of Wako Pure Chemical Industries, Ltd.), and then dried. The dried product was stacked and packed on a column previously packed with 1 kg of silica gel and eluted with a chloroform / methanol mixture (30: 1) to collect a glabridin-containing fraction. The solvent of this fraction was distilled off under reduced pressure to obtain 5.8 g of a solid substance, which was then dissolved in a small amount of methanol, sprinkled with reverse phase silica gel (ODSG-3, product of Mito Chemical Research Laboratory) and dried. , And stacked and packed on a column previously packed with 800 g of reverse phase silica gel. A water-acetonitrile mixture (30:70) was passed through this column as an elution solvent, and a glabridin-containing fraction was collected. The solvent was distilled off from this fraction under reduced pressure, the obtained solid substance (4.3 g) was dissolved in 40 ml of acetone, and the mixture was allowed to stand at 5 ° C. for 3 days to obtain 3.8 g of crystals of glabridin. In each of the following examples, crystals of the above purified glabridin were used as glabridin.
【0015】実施例1 表1の処方により、化粧水を製造した。この場合、まず
エタノール/1,3-ブチレングリコール混合液に上記製造
例によるグラブリジン結晶を溶解し、さらに界面活性剤
(モノラウリン酸ポリオキシソルビタン;20E.
O.)、香料およびパラオキシ安息香酸エステルを加え
て溶解した後、精製水およびその他の成分を加え、撹拌
して均一化した。なお、活性酸素消去剤として用いた3
種類の植物抽出物は、いずれも原料植物の粗砕物に50
重量%のエタノール水溶液を加え、ときどき撹拌しなが
ら室温で7日間放置したのち清澄濾過して得られたエキ
スである。Example 1 A lotion was prepared according to the formulation shown in Table 1. In this case, the glabridin crystals according to the above-mentioned production example are first dissolved in an ethanol / 1,3-butylene glycol mixed solution, and a surfactant (polyoxysorbitan monolaurate; 20E.
O.), perfume and para-oxybenzoic acid ester were added and dissolved, then purified water and other components were added and stirred to homogenize. In addition, 3 used as an active oxygen scavenger
50 kinds of plant extracts are used for the coarsely crushed raw materials.
An extract obtained by adding a wt% ethanol aqueous solution, allowing the mixture to stand at room temperature for 7 days with occasional stirring, and then clarified by filtration.
【0016】[0016]
【表1】化粧水処方(単位:重量%) 注1:処方4は比較例[Table 1] Lotion formulation (unit:% by weight) Note 1: Prescription 4 is a comparative example
【0017】次に、上記各化粧水について下記の方法に
より使用効果の試験を行なった。 紅斑抑制効果試験法:褐色モルモットの背部を除毛して
そこにオクソラレンの0.1%溶液を塗布し、30分後
にUVA 1J/cm2を照射した。照射直後、照射部位につ
き2cm×2cm大の5区画を決め、各区画に、 A:上記化粧水そのまま B:上記化粧水の処方1〜3からグラブリジンを除いた
化粧水 C:上記化粧水の処方1〜3から活性酸素消去剤を除い
た化粧水 D:上記化粧水の処方1〜3からグラブリジンと活性酸
素消去剤の両方を除いた化粧水 E:処方4の化粧水 のいずれかを塗布し、24時間後の紅斑抑制効果を肉眼
観察により判定した(PUVA処理しない皮膚の色を基
準色とする)。Next, the use effect of each of the above lotions was tested by the following method. Test method for erythema suppressing effect: The back of a brown guinea pig was shaved, a 0.1% solution of oxsoralen was applied thereto, and after 30 minutes, UVA 1 J / cm 2 was irradiated. Immediately after the irradiation, 5 sections with a size of 2 cm × 2 cm are determined for each irradiation site, and in each section, A: the above lotion as it is B: lotion without glabridin from the above formulations 1 to 3 C: the above lotion formulation Toner lotion from which active oxygen scavenger is removed from 1 to 3 D: Toner lotion from which both glabridin and active oxygen scavenger are removed from formulations 1 to 3 E: Apply one of the lotions of formulation 4 After 24 hours, the erythema-suppressing effect was determined by visual observation (using the color of the skin not treated with PUVA as the reference color).
【0018】色素沈着抑制効果試験法:紅斑抑制効果試
験法に準じてPUVA処理した褐色モルモットにつき、
1週間後、色素の沈着が認められた部位を2cm×2cmの
5区画に分け、各区画に、先に示したA〜Eの化粧水の
いずれかを朝夕各1回、10日間連続で塗布し、10日
後の色素沈着抑制効果を肉眼観察により判定した(PU
VA処理しない皮膚の色を基準色とする)。Pigmentation-inhibiting effect test method: According to the erythema-inhibiting effect test method, a brown guinea pig treated with PUVA was prepared.
After 1 week, the area where pigmentation was observed was divided into 5 sections of 2 cm x 2 cm, and one of the above-mentioned lotions A to E was applied to each section once every morning and evening for 10 consecutive days. After 10 days, the effect of suppressing pigmentation was judged by visual observation (PU
The standard color is the color of the skin that is not VA-treated).
【0019】その結果を表2および表3に示す。グラブ
リジンおよび活性酸素消去剤の両方を含有する本発明化
粧水は、グラブリジンおよび活性酸素消去剤のいずれを
も含まない化粧水(D)やアスコルビン酸リン酸マグネ
シウムを用いた処方4の比較例化粧水(E)よりも、紫
外線による紅斑を抑制する効果および色素沈着抑制効果
において顕著に優れていることがわかる。また、本発明
の化粧水は、グラブリジンと活性酸素消去剤の一方のみ
を含む化粧水の塗布例(B,C)と比較しても優れた使
用効果を示すことがわかる。なお、炎症その他の皮膚障
害は観察されなかった。The results are shown in Tables 2 and 3. The lotion of the present invention containing both glabridine and an active oxygen scavenger is a lotion (D) containing neither glabridin nor an active oxygen scavenger or a comparative example lotion of Formulation 4 using magnesium ascorbate phosphate. It can be seen that the effect of suppressing erythema due to ultraviolet rays and the effect of suppressing pigmentation are remarkably superior to those of (E). Further, it can be seen that the lotion of the present invention shows an excellent use effect even when compared with the application examples (B, C) of lotion containing only one of glabridin and the active oxygen scavenger. No inflammation or other skin disorders were observed.
【0020】[0020]
【表2】 化粧水基本処方 紅斑抑制効果 処方1 PUVA処理しない皮膚≧A≫C=B≫D=E 処方2 PUVA処理しない皮膚=A≫C≧B≫D=E 処方3 PUVA処理しない皮膚≧A≫C≧B≫D=E[Table 2] Cosmetic lotion basic formulation Erythema suppressing effect Formulation 1 PUVA-untreated skin ≧ A >> C = B >> D = E Formulation 2 PUVA-untreated skin = A >> C ≧ B >> D = E Formulation 3 PUVA-untreated skin ≧ A >> C ≧ B >> D = E
【0021】[0021]
【表3】 化粧水基本処方 色素沈着抑制効果 処方1 PUVA処理しない皮膚=A≫C>B>E>D 処方2 PUVA処理しない皮膚=A≫C>E>B>D 処方3 PUVA処理しない皮膚=A≫C>E>B>D[Table 3] Basic lotion formulation Pigmentation suppression effect Formulation 1 Skin not treated with PUVA = A >>C>B>E> D Formulation 2 Skin not treated with PUVA = A >>C>E>B> D Formulation 3 Skin not treated with PUVA = A >>C>E>B> D
【0022】実施例2 表4の処方によりクリームを製造した。製造に際して
は、まず同表群の原料を70℃で溶解し、原料と混
合した後、78℃にした。次いでこれを、75℃に加熱
した原料へ撹拌しながら徐々に加え、予備乳化を行っ
た。その後ホモジナイザーにかけて乳化を完全に行い、
50℃に冷却後、を添加し、30℃まで冷却した。な
お、処方8は比較例である。Example 2 A cream was produced according to the formulation shown in Table 4. At the time of production, the raw materials in the same table group were first melted at 70 ° C., mixed with the raw materials, and then heated to 78 ° C. Next, this was gradually added to the raw material heated to 75 ° C. with stirring to carry out preliminary emulsification. After that, apply a homogenizer to completely emulsify,
After cooling to 50 ° C, was added and cooled to 30 ° C. In addition, the prescription 8 is a comparative example.
【0023】[0023]
【表4】クリーム処方(単位:重量%) [Table 4] Cream prescription (unit:% by weight)
【0024】(注1)界面活性剤A:自己乳化型モノス
テアリン酸グリセリン 界面活性剤B:モノステアリン酸ソルビタン カルボキシビニルポリマー:1%水溶液(Note 1) Surfactant A: self-emulsifying glycerin monostearate Surfactant B: sorbitan monostearate carboxyvinyl polymer: 1% aqueous solution
【0025】次に、上記各クリームについて下記の方法
により使用効果の試験を行なった。 色素沈着抑制効果試験法:褐色モルモットの背部を除毛
し、除毛部位を2cm×2cmの区画4区画に分画し、そこ
に、1日当たりUVBを1J/cm2で2日間照射した。4
日後に色素の沈着が認められたので、各区画に A:上記クリームそのまま B:上記クリームの処方からグラブリジンのみを除いた
クリーム C:上記クリームの処方から活性酸素消去剤を除いたク
リーム D:処方7のクリーム のいずれかを、1日1回、10日間連続で塗布し、10
日後に色素沈着抑制効果を肉眼観察により判定した(U
VB処理しない皮膚の色を基準色とする)。Next, each of the above creams was tested for its use effect by the following method. Pigmentation suppression effect test method: The back of a brown guinea pig was depilated, and the depilated site was divided into 4 sections of 2 cm x 2 cm, which were irradiated with UVB at 1 J / cm 2 for 2 days. Four
Since pigment deposition was observed after a day, A: the above cream as it was in each compartment B: cream excluding glabridin from the above cream formulation C: cream excluding the active oxygen scavenger from the above cream formulation D: formulation Apply one of the 7 creams once a day for 10 consecutive days,
After a day, the effect of suppressing pigmentation was judged by visual observation (U
The standard color is the color of the skin that is not VB treated).
【0026】紅斑抑制効果試験法:色素沈着抑制効果試
験法に準じてUVB処理した褐色モルモットの背部の各
区画に、先に示したA〜Dのクリームを塗布し、24時
間後に紅斑の抑制効果を肉眼観察により判定した(UV
B処理しない皮膚の色を基準色とする)。Erythema inhibitory effect test method: According to the pigmentation inhibitory effect test method, the above-mentioned creams A to D were applied to each compartment on the back of the UVB-treated brown guinea pig, and after 24 hours, the effect of suppressing erythema. Was judged by visual observation (UV
B: The color of the skin not treated is the standard color).
【0027】その結果を表5および表6に示す。グラブ
リジンと活性酸素消去剤を含有する本発明のクリームを
塗布した場合をグラブリジンまたは活性酸素消去剤を除
いたクリームの塗布例と比較すると、前者は紫外線によ
る紅斑抑制効果および色素沈着抑制効果のいずれにおい
ても後者より優れていることがわかった。また、炎症そ
の他の皮膚障害は観察されなかった。The results are shown in Tables 5 and 6. Comparing the case of applying the cream of the present invention containing glaburidine and the active oxygen scavenger with the application example of the cream excluding glabridine or the active oxygen scavenger, the former is either the erythema suppressing effect and the pigmentation suppressing effect by ultraviolet rays. Was also found to be superior to the latter. In addition, no inflammation or other skin disorders were observed.
【0028】[0028]
【表5】 クリーム基本処方 色素沈着抑制効果 処方5 UVB処理しない皮膚=A≫C>B>D 処方6 UVB処理しない皮膚≧A≫C>B>D 処方7 UVB処理しない皮膚≧A≫C>B>D[Table 5] Cream basic formulation Pigmentation suppressing effect Formulation 5 UVB-untreated skin = A >>C>B> D Formulation 6 UVB-untreated skin ≧ A >>C>B> D Formulation 7 UVB-untreated skin ≧ A >>C>B> D
【0029】[0029]
【表6】 クリーム基本処方 紅斑抑制効果 処方5 UVB処理しない皮膚≧A≫C≧B≫D 処方6 UVB処理しない皮膚≧A≫C≧B≫D 処方7 UVB処理しない皮膚≧A≫C≧B≫D[Table 6] Cream basic formulation Erythema suppressing effect Formulation 5 UVB-untreated skin ≧ A >> C ≧ B >> D Formulation 6 UVB-untreated skin ≧ A >> C ≧ B >> D Formulation 7 UVB-untreated skin ≧ A >> C ≧ B ≫ D
【0030】[0030]
【発明の効果】上述のように、グラブリジンと活性酸素
消去剤を含有する本発明の化粧料は紫外線による皮膚の
炎症および色素沈着を効果的に防止することができ、好
ましくない副作用も認められない。Industrial Applicability As described above, the cosmetic of the present invention containing glabridin and an active oxygen scavenger can effectively prevent skin inflammation and pigmentation due to ultraviolet rays, and no undesirable side effects are observed. .
Claims (1)
性酸素消去剤を含有することを特徴とする皮膚化粧料。1. A skin cosmetic, which comprises glabridin and an active oxygen scavenger as essential components.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP06252193A JP3195684B2 (en) | 1993-03-01 | 1993-03-01 | Skin cosmetics |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP06252193A JP3195684B2 (en) | 1993-03-01 | 1993-03-01 | Skin cosmetics |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH06256152A true JPH06256152A (en) | 1994-09-13 |
JP3195684B2 JP3195684B2 (en) | 2001-08-06 |
Family
ID=13202579
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP06252193A Expired - Lifetime JP3195684B2 (en) | 1993-03-01 | 1993-03-01 | Skin cosmetics |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP3195684B2 (en) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06256151A (en) * | 1993-03-01 | 1994-09-13 | Maruzen Pharmaceut Co Ltd | Skin cosmetic |
JPH0977636A (en) * | 1995-09-14 | 1997-03-25 | Mikimoto Pharmaceut Co Ltd | Beautifying and whitening cosmetic |
JPH10182333A (en) * | 1996-12-20 | 1998-07-07 | Noevir Co Ltd | Antimicrobial and low-irritation cosmetic |
JPH10194915A (en) * | 1997-01-14 | 1998-07-28 | Noevir Co Ltd | Antimicrobial and low-irritating cosmetic |
WO2000042976A1 (en) * | 1999-01-20 | 2000-07-27 | Faith Co., Ltd. | Cosmetics |
JP2000319154A (en) * | 1999-05-06 | 2000-11-21 | Nippon Menaade Keshohin Kk | Phototoxicity inhibitor |
WO2001019331A1 (en) * | 1999-09-10 | 2001-03-22 | Henkel Kommanditgesellschaft Auf Aktien | Skin care product containing a catalase |
JP2002029992A (en) * | 2000-05-08 | 2002-01-29 | Nonogawa Shoji Kk | Activity accelerator for active oxygen scavenging enzyme |
JP2003306418A (en) * | 2003-05-26 | 2003-10-28 | Pola Chem Ind Inc | Sunscreening cosmetic |
JP2006104117A (en) * | 2004-10-04 | 2006-04-20 | National Institute Of Advanced Industrial & Technology | Skin ageing-preventing effect-improving agent, method for producing the same, skin ageing-preventing composition using the same and skin care preparation for external use containing the same |
JP2006273736A (en) * | 2005-03-29 | 2006-10-12 | A Pharma Kindai Co Ltd | Bleaching agent based on antioxidative action of neohesperidin or chromatosis improving agent |
US9199260B2 (en) | 2003-02-28 | 2015-12-01 | Carlisle Fluid Technologies, Inc. | Repeatable mounting unit for automatic spray device |
-
1993
- 1993-03-01 JP JP06252193A patent/JP3195684B2/en not_active Expired - Lifetime
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06256151A (en) * | 1993-03-01 | 1994-09-13 | Maruzen Pharmaceut Co Ltd | Skin cosmetic |
JPH0977636A (en) * | 1995-09-14 | 1997-03-25 | Mikimoto Pharmaceut Co Ltd | Beautifying and whitening cosmetic |
JPH10182333A (en) * | 1996-12-20 | 1998-07-07 | Noevir Co Ltd | Antimicrobial and low-irritation cosmetic |
JPH10194915A (en) * | 1997-01-14 | 1998-07-28 | Noevir Co Ltd | Antimicrobial and low-irritating cosmetic |
WO2000042976A1 (en) * | 1999-01-20 | 2000-07-27 | Faith Co., Ltd. | Cosmetics |
JP2000319154A (en) * | 1999-05-06 | 2000-11-21 | Nippon Menaade Keshohin Kk | Phototoxicity inhibitor |
WO2001019331A1 (en) * | 1999-09-10 | 2001-03-22 | Henkel Kommanditgesellschaft Auf Aktien | Skin care product containing a catalase |
JP2002029992A (en) * | 2000-05-08 | 2002-01-29 | Nonogawa Shoji Kk | Activity accelerator for active oxygen scavenging enzyme |
US9199260B2 (en) | 2003-02-28 | 2015-12-01 | Carlisle Fluid Technologies, Inc. | Repeatable mounting unit for automatic spray device |
JP2003306418A (en) * | 2003-05-26 | 2003-10-28 | Pola Chem Ind Inc | Sunscreening cosmetic |
JP2006104117A (en) * | 2004-10-04 | 2006-04-20 | National Institute Of Advanced Industrial & Technology | Skin ageing-preventing effect-improving agent, method for producing the same, skin ageing-preventing composition using the same and skin care preparation for external use containing the same |
JP2006273736A (en) * | 2005-03-29 | 2006-10-12 | A Pharma Kindai Co Ltd | Bleaching agent based on antioxidative action of neohesperidin or chromatosis improving agent |
JP4654060B2 (en) * | 2005-03-29 | 2011-03-16 | 株式会社 ア・ファーマ近大 | Whitening agent or pigmentation ameliorating agent based on the antioxidant action of neohesperidin |
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