JPH04248982A - Production of bacteria capable of photosynthesis containing highly unsaturated fatty acid - Google Patents
Production of bacteria capable of photosynthesis containing highly unsaturated fatty acidInfo
- Publication number
- JPH04248982A JPH04248982A JP3033320A JP3332091A JPH04248982A JP H04248982 A JPH04248982 A JP H04248982A JP 3033320 A JP3033320 A JP 3033320A JP 3332091 A JP3332091 A JP 3332091A JP H04248982 A JPH04248982 A JP H04248982A
- Authority
- JP
- Japan
- Prior art keywords
- unsaturated fatty
- highly unsaturated
- fatty acids
- fatty acid
- photosynthetic bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 25
- 235000021122 unsaturated fatty acids Nutrition 0.000 title claims abstract description 25
- 150000004670 unsaturated fatty acids Chemical class 0.000 title claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- 230000029553 photosynthesis Effects 0.000 title abstract 2
- 238000010672 photosynthesis Methods 0.000 title abstract 2
- 230000000243 photosynthetic effect Effects 0.000 claims description 26
- 239000003921 oil Substances 0.000 claims description 15
- 239000003925 fat Substances 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 4
- 241000190932 Rhodopseudomonas Species 0.000 abstract description 4
- 241000190967 Rhodospirillum Species 0.000 abstract description 2
- 239000010685 fatty oil Substances 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 19
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 11
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
- 150000003904 phospholipids Chemical class 0.000 description 10
- 235000019197 fats Nutrition 0.000 description 9
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 8
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 8
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 8
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 150000002632 lipids Chemical class 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 5
- 235000014113 dietary fatty acids Nutrition 0.000 description 5
- 229930195729 fatty acid Natural products 0.000 description 5
- 239000000194 fatty acid Substances 0.000 description 5
- 150000004665 fatty acids Chemical class 0.000 description 5
- 235000019688 fish Nutrition 0.000 description 5
- 235000014593 oils and fats Nutrition 0.000 description 5
- 229940090949 docosahexaenoic acid Drugs 0.000 description 4
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 4
- KPNOURUYPWFAPT-UHFFFAOYSA-A C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+] Chemical compound C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].C(C=CCCC)(=O)[O-].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+] KPNOURUYPWFAPT-UHFFFAOYSA-A 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 229940066279 eicosapentaenoate Drugs 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 241000238582 Artemia Species 0.000 description 1
- 241000238366 Cephalopoda Species 0.000 description 1
- 241000555825 Clupeidae Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000191023 Rhodobacter capsulatus Species 0.000 description 1
- 241000190984 Rhodospirillum rubrum Species 0.000 description 1
- 241000134686 Rhodospirillum rubrum ATCC 11170 Species 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- 241000269851 Sarda sarda Species 0.000 description 1
- 241000269821 Scombridae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241001504592 Trachurus trachurus Species 0.000 description 1
- ATBOMIWRCZXYSZ-XZBBILGWSA-N [1-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-3-hexadecanoyloxypropan-2-yl] (9e,12e)-octadeca-9,12-dienoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COP(O)(=O)OCC(O)CO)OC(=O)CCCCCCC\C=C\C\C=C\CCCCC ATBOMIWRCZXYSZ-XZBBILGWSA-N 0.000 description 1
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940053200 antiepileptics fatty acid derivative Drugs 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000020640 mackerel Nutrition 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000004053 quinones Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000019512 sardine Nutrition 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 235000003441 saturated fatty acids Nutrition 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- -1 unsaturated fatty acid phospholipids Chemical class 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】0001
【産業上の利用分野】本発明は、高度不飽和脂肪酸含有
光合成細菌を製造する方法に関する。該光合成細菌は食
品、飼料、肥料及び医薬品、農業、水産、化成品などの
分野において有用である。FIELD OF THE INVENTION The present invention relates to a method for producing photosynthetic bacteria containing highly unsaturated fatty acids. The photosynthetic bacteria are useful in fields such as food, feed, fertilizer, medicine, agriculture, fisheries, and chemical products.
【０００２】0002
【従来の技術】光合成細菌はその菌体中に良質のタンパ
ク質を豊富に含んでおり、また細菌性葉緑素やカロチン
系色素、ビタミン含量も多く、養殖漁業において、重要
な飼料となり、既に市販もされている。更に食品、肥料
としての利用も考えられている。[Prior Art] Photosynthetic bacteria contain an abundance of high-quality protein in their cells, and also contain a large amount of bacterial chlorophyll, carotene pigments, and vitamins, making them an important feed for aquaculture and fisheries, and are already commercially available. ing. It is also being considered for use as food and fertilizer.
【０００３】0003
【発明が解決しようとする課題】しかしながら、かかる
光合成細菌が有する脂肪酸としては炭素数１６〜１８の
飽和脂肪酸及び／又はモノエン脂肪酸を主成分とするも
のであり、炭素数１８以上かつ不飽和結合２以上の不飽
和脂肪酸（以下、高度不飽和脂肪酸と称す）、特に近年
稚仔魚の増養殖に必須とされているエイコサペンタエン
酸やドコサヘキサエン酸を全く含有していない。本発明
の目的は、高度不飽和脂肪酸含有光合成細菌を工業的に
効率良く、短時間で、容易に得ることのできる製造法を
提供することにある。[Problems to be Solved by the Invention] However, the fatty acids possessed by such photosynthetic bacteria are mainly composed of saturated fatty acids and/or monoenoic fatty acids having 16 to 18 carbon atoms, and have at least 18 carbon atoms and 2 unsaturated bonds. It does not contain any of the above-mentioned unsaturated fatty acids (hereinafter referred to as highly unsaturated fatty acids), especially eicosapentaenoic acid and docosahexaenoic acid, which have been essential in growing and cultivating young fish in recent years. An object of the present invention is to provide a method for producing highly unsaturated fatty acid-containing photosynthetic bacteria industrially efficiently, quickly, and easily.
【０００４】0004
【課題を解決するための手段】本発明を概説すれば、本
発明は高度不飽和脂肪酸含有光合成細菌の製造法に関す
る発明であって、光合成細菌を、高度不飽和脂肪酸及び
／又はその誘導体を含む油脂を添加した培地中で培養す
ることを特徴とする。[Means for Solving the Problems] To summarize the present invention, the present invention relates to a method for producing photosynthetic bacteria containing highly unsaturated fatty acids, and the present invention relates to a method for producing photosynthetic bacteria containing highly unsaturated fatty acids and/or derivatives thereof. It is characterized by being cultured in a medium supplemented with fats and oils.
【０００５】本発明者らは、上記の目的に合った製造法
を見出すために鋭意研究の結果、培地中に高度不飽和脂
肪酸及び／又はその誘導体を含む油脂を添加して培養し
た光合成細菌の脂質について、次のような知見を得た。
すなわち、■光合成細菌の総脂質含有量は乾物換算で６
〜１２重量％と高く、そのうち９０％以上がリン脂質で
あること、■リン脂質の脂肪酸組成を見ても高度不飽和
脂肪酸が１０〜３０重量％と高いこと、■本発明で用い
る光合成細菌の増殖速度は著しく速く、多量の菌体を極
めて短時間のうちに得ることができること、等である。
本発明は、以上の知見に基づいて完成されたものである
。[0005] As a result of intensive research in order to find a production method that meets the above objectives, the present inventors have discovered that photosynthetic bacteria were cultured by adding fats and oils containing highly unsaturated fatty acids and/or derivatives thereof to the medium. The following findings regarding lipids were obtained. In other words, the total lipid content of photosynthetic bacteria is 6 on a dry matter basis.
~12% by weight, of which more than 90% is phospholipids; ■ Looking at the fatty acid composition of phospholipids, highly unsaturated fatty acids are high at 10 to 30% by weight; ■ The photosynthetic bacteria used in the present invention The growth rate is extremely fast, and a large amount of bacterial cells can be obtained in an extremely short period of time. The present invention was completed based on the above findings.
【０００６】本発明で用いる高度不飽和脂肪酸及び／又
はその誘導体を含む油脂としては、好ましくは総脂肪酸
中の高度不飽和脂肪酸の占める割合が１０重量％以上の
ものが良く、このようなものの例を挙げるとイワシ、サ
バ、アジ等の青背魚より抽出した油脂、マグロやカツオ
等の大型海産魚の眼窩脂肪由来の油脂、微生物や藻類由
来の油脂、イカ等の肝臓より抽出した海産物由来の油脂
かどが好ましい例として挙げられる。The fats and oils containing highly unsaturated fatty acids and/or their derivatives used in the present invention are preferably those in which the proportion of highly unsaturated fatty acids in the total fatty acids is 10% by weight or more, and examples of such oils are as follows: These include oils and fats extracted from blue-backed fish such as sardines, mackerel, and horse mackerel, oils and fats derived from the orbital fat of large marine fish such as tuna and bonito, oils and fats derived from microorganisms and algae, and oils and fats derived from marine products extracted from the livers of squid and others. A preferred example is the corner.
【０００７】本発明で使用できる光合成細菌は、特に属
、種あるいは株などを限定するものではないが、通常は
、ロドシュードモナス（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎ
ａｓ）属、ロドスピリルム（Ｒｈｏｄｏｓｐｉｒｉｌｌ
ｕｍ）属などに分類される光合成細菌を用いることがで
きる。これらの光合成細菌については公的微生物寄託機
関等にて容易に入手できる。例えば上記のロドシュード
モナスに属する光合成細菌の例としてロドシュードモナ
ス・キャプシュレイタ（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎ
ａｓ ｃａｐｓｕｌａｔａ ）ＦＥＲＭ Ｐ−８７
９，ＡＴＣＣ−１７０１５及びロドスピリルム・ルブル
ム（Ｒｈｏｄｏｓｐｉｒｉｌｌｕｍｒｕｂｒｕｍ）ＡＴ
ＣＣ−１１１７０等を挙げることができる。The photosynthetic bacteria that can be used in the present invention are not particularly limited in genus, species, or strain, but are usually Rhodopseudomonas.
as) genus, Rhodospirillum
Photosynthetic bacteria classified into the genus Um) can be used. These photosynthetic bacteria can be easily obtained from public microorganism depositories. For example, as an example of the photosynthetic bacteria belonging to Rhodopseudomonas mentioned above, Rhodopseudomonas capsulita
as capsulata) FERM P-87
9, ATCC-17015 and Rhodospirillum rubrum AT
CC-11170 etc. can be mentioned.
【０００８】本発明の実施に当っては、光合成細菌を常
法により例えば培地で培養し、これを遠心分離法などで
集め、洗浄、必要に応じ乾燥して微生物粉末を得る。こ
の際の培地としては例えば下記表１に示す組成の培地を
調製することができる。％は重量％である。In carrying out the present invention, photosynthetic bacteria are cultured in a conventional manner, for example, in a medium, collected by centrifugation, washed, and optionally dried to obtain a microbial powder. As the culture medium at this time, for example, a culture medium having the composition shown in Table 1 below can be prepared. % is by weight.
【０００９】[0009]
【表１】
表 １
酢酸ナトリウム ０
．３％ リン酸一カリウム
０．０５％ 塩化マグネシウム
０．０２％ 塩化ナトリウム
０．０１％ 塩化第二鉄
０．０００１％
硫酸アンモニウム ０
．０３％ 酵母エキス
０．３％ ペプトン
０．３％────────
───────────────────
ｐＨ ７
．０[Table 1] Table 1
Sodium acetate 0
．． 3% monopotassium phosphate
0.05% magnesium chloride
0.02% sodium chloride
0.01% ferric chloride
0.0001%
Ammonium sulfate 0
．． 03% yeast extract
0.3% peptone
0.3%────────
────────────────────
pH 7
．． 0
【００１０】培地中に添加する油脂としては、脂肪
酸の遊離型、エステル型、塩等の誘導体を含む油脂を用
いることができる。より好ましくは高度不飽和脂肪酸の
塩、例えばカリウム塩、ナトリウム塩、カルシウム塩等
を含む油脂を用いることができる。[0010] As the fats and oils added to the culture medium, fats and oils containing fatty acid derivatives such as free type, ester type, and salt can be used. More preferably, oils and fats containing salts of highly unsaturated fatty acids, such as potassium salts, sodium salts, calcium salts, etc. can be used.
【００１１】添加する油脂は、培地に対し０．００１〜
１０重量％、好ましくは０．０１〜１．０重量％を添加
することが、光合成細菌の増殖及び高度不飽和脂肪酸の
取込み率から見て望ましい。また光合成細菌の培養時に
乳化剤を添加しても良い。[0011] The amount of oil and fat to be added is 0.001~
It is desirable to add 10% by weight, preferably 0.01 to 1.0% by weight, in view of the growth of photosynthetic bacteria and the uptake rate of highly unsaturated fatty acids. Furthermore, an emulsifier may be added during culturing of photosynthetic bacteria.
【００１２】培養終了後集菌により菌体あるいは水分を
除去した乾燥菌体を得ることができる。このような菌体
中には高度不飽和脂肪酸が含有されている。なお、菌体
に含有された高度不飽和脂肪酸の総量は、培地に添加さ
れた油脂の種類や量、培養温度や時間等により変化する
が、およそ１．０〜３．０重量％である。[0012] After completion of the culture, bacterial cells or dried bacterial cells from which moisture has been removed can be obtained by bacterial collection. Such bacterial cells contain highly unsaturated fatty acids. The total amount of highly unsaturated fatty acids contained in the bacterial cells varies depending on the type and amount of fats and oils added to the medium, culture temperature, time, etc., but is approximately 1.0 to 3.0% by weight.
【００１３】また所望によりこの光合成細菌菌体から、
有機溶剤などを用いて脂質抽出を行い、脱溶剤後、アセ
トン分画を行い、アセトンに不溶な物質を集めれば、リ
ン脂質の粗分画物を得ることができる。[0013] If desired, from this photosynthetic bacterial cell,
A crude fraction of phospholipids can be obtained by extracting lipids using an organic solvent, removing the solvent, fractionating with acetone, and collecting substances insoluble in acetone.
【００１４】このようにして得られた高度不飽和脂肪酸
含有光合成細菌菌体は、シオミズツボワムシやアルテミ
ア等の稚仔魚用飼料生物のエサ、あるいは直接稚仔魚用
飼料として使用できる。更に食品や動物用飼料、土壌改
善用にも使用が可能である。一方該菌体より抽出した高
度不飽和脂肪酸リン脂質は、種々の疾病に対する医薬品
、化粧品素材、化成品原料としての利用が可能である。The photosynthetic bacterial cells containing highly unsaturated fatty acids obtained in this manner can be used as feed for feed organisms for young fish, such as the rotifer and Artemia, or directly as feed for young fish. Furthermore, it can be used for food, animal feed, and soil improvement. On the other hand, highly unsaturated fatty acid phospholipids extracted from the bacterial cells can be used as medicines for various diseases, cosmetic materials, and chemical raw materials.
【００１５】[0015]
【実施例】以下、本発明を実施例により更に具体的に説
明するが、本発明はこれら実施例に限定されない。なお
、例中の％は特に断らない限り重量％を意味する。[Examples] The present invention will be explained in more detail with reference to Examples below, but the present invention is not limited to these Examples. Note that % in the examples means % by weight unless otherwise specified.
【００１６】実施例１
ロドシュードモナス・キャプシュレイタ ＡＴＣＣ−
１７０１５を、表１に示した培地に、マグロ眼窩脂肪よ
り抽出して得られた脂肪酸カリウム塩からなる油脂（該
油脂中ドコサヘキサエン酸カリウム３０％、エイコサペ
ンタエン酸カリウム８．４％を含有）０．１％を含有せ
しめ、ｐＨ７に調整した培地中で１５℃にて１０リット
ル好気培養し、培養物を遠心分離して集め、洗浄して菌
体１００ｇを得た。得られた菌体１００ｇをクロロホル
ム：メタノール＝２：１混合溶剤で抽出し、８ｇの抽出
脂質を得た。この脱溶剤後の総脂質にアセトン４００ｍ
ｌを加え、冷却しながらかくはんを行い、糖脂質、キノ
ンを分別ろ過によりアセトン不溶分を回収した。得られ
たアセトン不溶分をクロロホルムに溶解し、次にこの溶
液をシリカゲルカラムクロマトグラフィーにかけ、クロ
ロホルム、クロロホルム：メタノール＝２：１、クロロ
ホルム：メタノール＝１：１及びメタノールで順次展開
した。得られた各フラクションをＴＬＣでチェックし、
目的とするリン脂質のフラクションをいくつか見出し、
それらを回収し、脱溶剤したところ約７ｇのリン脂質が
得られた。得られたリン脂質の組成はＴＬＣ分析の結果
、ホスファチジルグリセロール（７０％）及びホスファ
チジルエタノールアミン（２０％）が主成分であり、ま
たこれらの脂質の構成高度不飽和脂肪酸はドコサヘキサ
エン酸１８．５％、エイコサペンタエン酸５．６％であ
つた。Example 1 Rhodopseudomonas capsulata ATCC-
17015 was added to the medium shown in Table 1, and 0.0% of an oil or fat (containing 30% potassium docosahexaenoate and 8.4% potassium eicosapentaenoate in the oil) was prepared from fatty acid potassium salt extracted from tuna orbital fat. A 10 liter aerobic culture was carried out at 15° C. in a medium containing 1% and adjusted to pH 7, and the culture was collected by centrifugation and washed to obtain 100 g of bacterial cells. 100 g of the obtained bacterial cells were extracted with a mixed solvent of chloroform:methanol=2:1 to obtain 8 g of extracted lipid. Add 400ml of acetone to the total lipids after this solvent removal.
1 was added, stirred while cooling, and acetone-insoluble components were collected by differential filtration of glycolipids and quinones. The resulting acetone-insoluble matter was dissolved in chloroform, and this solution was then subjected to silica gel column chromatography, which was developed sequentially with chloroform, chloroform:methanol=2:1, chloroform:methanol=1:1, and methanol. Check each fraction obtained by TLC,
We found several target phospholipid fractions,
When they were collected and the solvent was removed, about 7 g of phospholipids were obtained. As a result of TLC analysis, the composition of the obtained phospholipids was determined to be mainly composed of phosphatidylglycerol (70%) and phosphatidylethanolamine (20%), and the polyunsaturated fatty acids that constituted these lipids were 18.5% docosahexaenoic acid. , eicosapentaenoic acid 5.6%.
【００１７】実施例２
上記実施例１に用いた菌株と同じ菌株を、添加する油脂
としてエイコサペンタエン酸０．１％を用い、上記実施
例１と同様の方法で培養して菌体６４ｇを得た。該菌体
を実施例１と同方法にて抽出したところ、リン脂質６．
８ｇを得た。該リン脂質の構成高度不飽和脂肪酸はエイ
コサペンタエン酸２１％であった。Example 2 The same bacterial strain as used in Example 1 was cultured in the same manner as in Example 1 using 0.1% eicosapentaenoic acid as the added oil to obtain 64 g of bacterial cells. Ta. When the bacterial cells were extracted using the same method as in Example 1, phospholipids 6.
8g was obtained. The highly unsaturated fatty acid constituting the phospholipid was 21% eicosapentaenoic acid.
【００１８】実施例３
上記実施例１に用いた菌株と同じ菌株を、添加する油脂
としてエイコサペンタエン酸カリウム０．１％及びドコ
サヘキサエン酸カリウム０．１％を用い、上記実施例１
と同様の方法で培養して得た光合成細菌菌体の重量を測
定したところ６６．５ｇであった。該光合成細菌の含有
する高度不飽和脂肪酸は、ドコサヘキサエン酸８．５％
、エイコサペンタエン酸１７．２％であった。Example 3 The same strain as that used in Example 1 was used, and 0.1% of potassium eicosapentaenoate and 0.1% of potassium docosahexaenoate were used as the added oils.
The weight of photosynthetic bacterial cells obtained by culturing in the same manner as above was measured and was 66.5 g. The highly unsaturated fatty acids contained in this photosynthetic bacterium include 8.5% docosahexaenoic acid.
, eicosapentaenoic acid 17.2%.
【００１９】実施例４
ロドスピリルム・ルブルム ＡＴＣＣ−１１１７０を
、添加する油脂としてエイコサペンタエン酸０．１％を
用い、上記実施例１と同様の方法で培養して菌体５３ｇ
を得た。該菌体を実施例１と同方法にて抽出したところ
、リン脂質５．８ｇを得た。該リン脂質の構成高度不飽
和脂肪酸はエイコサペンタエン酸１９％であった。Example 4 Rhodospirillum rubrum ATCC-11170 was cultured in the same manner as in Example 1 above using 0.1% eicosapentaenoic acid as the added oil to obtain 53 g of bacterial cells.
I got it. When the bacterial cells were extracted in the same manner as in Example 1, 5.8 g of phospholipids were obtained. The highly unsaturated fatty acid constituting the phospholipid was 19% eicosapentaenoic acid.
【００２０】実施例５
ロドスピリウム・ルブルム ＡＴＣＣ−１１１７０を
、添加する油脂としてエイコサペンタエン酸カリウム０
．１％及びドコサヘキサエン酸カリウム０．１％を用い
、上記実施例１と同様の方法で培養して得た光合成細菌
菌体の重量を測定したところ５７．６ｇであった。該光
合成細菌の含有する高度不飽和脂肪酸は、ドコサヘキサ
エン酸７．４％、エイコサペンタエン酸１７．６％であ
った。Example 5 Rhodospirum rubrum ATCC-11170 was added with 0 potassium eicosapentaenoate as the fat and oil added.
．． The weight of photosynthetic bacterial cells obtained by culturing in the same manner as in Example 1 using 1% and 0.1% potassium docosahexaenoate was 57.6 g. The highly unsaturated fatty acids contained in the photosynthetic bacteria were 7.4% docosahexaenoic acid and 17.6% eicosapentaenoic acid.
【００２１】[0021]
【発明の効果】以上説明したように、本発明方法により
、高度不飽和脂肪酸含有光合成細菌を効率良く、短時間
で且つ容易に得ることが可能となった。[Effects of the Invention] As explained above, the method of the present invention makes it possible to efficiently obtain highly unsaturated fatty acid-containing photosynthetic bacteria in a short time and easily.
Claims (1)
／又はその誘導体を含む油脂を添加した培地中で培養す
ることを特徴とする高度不飽和脂肪酸含有光合成細菌の
製造法。1. A method for producing photosynthetic bacteria containing highly unsaturated fatty acids, which comprises culturing the photosynthetic bacteria in a medium supplemented with fats and oils containing highly unsaturated fatty acids and/or derivatives thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3033320A JP3044577B2 (en) | 1991-02-04 | 1991-02-04 | Production of photosynthetic bacteria containing polyunsaturated fatty acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3033320A JP3044577B2 (en) | 1991-02-04 | 1991-02-04 | Production of photosynthetic bacteria containing polyunsaturated fatty acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04248982A true JPH04248982A (en) | 1992-09-04 |
| JP3044577B2 JP3044577B2 (en) | 2000-05-22 |
Family
ID=12383270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3033320A Expired - Lifetime JP3044577B2 (en) | 1991-02-04 | 1991-02-04 | Production of photosynthetic bacteria containing polyunsaturated fatty acids |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3044577B2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994021140A1 (en) * | 1993-03-19 | 1994-09-29 | William Leslie Porter | Animal feed supplement |
| WO2001060977A1 (en) * | 2000-02-17 | 2001-08-23 | Biochem Industrial Co., Ltd. | Purple photosynthetic bacteria and health foods |
| JP2006513176A (en) * | 2002-12-12 | 2006-04-20 | ネーデルランドセ オルガニサティエ フォール トエゲパストナトールヴェテンシャッペリク オンデルゾエク ティエヌオー | Cholesterol-reducing preparations, food supplements and foods, and methods for their production |
| US7314732B2 (en) | 2000-02-17 | 2008-01-01 | Tfk Inc. | Drug and manufacturing method of same |
| EP1797169A4 (en) * | 2004-10-08 | 2008-11-19 | Coho Hydroclean Sa | A method of cultivating animals to develop a desired color and to increase their rate of growth |
-
1991
- 1991-02-04 JP JP3033320A patent/JP3044577B2/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994021140A1 (en) * | 1993-03-19 | 1994-09-29 | William Leslie Porter | Animal feed supplement |
| WO2001060977A1 (en) * | 2000-02-17 | 2001-08-23 | Biochem Industrial Co., Ltd. | Purple photosynthetic bacteria and health foods |
| US6797279B2 (en) | 2000-02-17 | 2004-09-28 | Biochem Industrial Co., Ltd. | Rhodopseudomonas capsulatas strain No. FERM BP-7434 for use in health foods |
| US7314732B2 (en) | 2000-02-17 | 2008-01-01 | Tfk Inc. | Drug and manufacturing method of same |
| JP2006513176A (en) * | 2002-12-12 | 2006-04-20 | ネーデルランドセ オルガニサティエ フォール トエゲパストナトールヴェテンシャッペリク オンデルゾエク ティエヌオー | Cholesterol-reducing preparations, food supplements and foods, and methods for their production |
| JP4814524B2 (en) * | 2002-12-12 | 2011-11-16 | ネーデルランドセ オルガニサティエ フォール トエゲパストナトールヴェテンシャッペリク オンデルゾエク ティエヌオー | Preparations that lower cholesterol |
| EP1797169A4 (en) * | 2004-10-08 | 2008-11-19 | Coho Hydroclean Sa | A method of cultivating animals to develop a desired color and to increase their rate of growth |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3044577B2 (en) | 2000-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2006345866A (en) | Method for forming arachidonic acid | |
| WO1996033263A1 (en) | Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms | |
| JPH05505726A (en) | Eicosapentaenoic acid and its production method | |
| JP2001505538A (en) | Microbial preparation containing omega-3-fatty acids for use as a prophylactic or therapeutic agent against animal parasitosis | |
| KR102202287B1 (en) | Biomass of the microalgae schizochytrium mangrovei and method for preparing same | |
| CN103667068A (en) | Arachidonic acid-rich oil produced by microorganism (unicellular fungal mortierella alpina) and preparation process thereof | |
| JP3506740B2 (en) | Method for culturing algae containing docosahexaenoic acid | |
| JP3354582B2 (en) | Method for producing omega-9 polyunsaturated fatty acid and lipid containing the same | |
| US20130309719A1 (en) | Heterotrophic microbial production of xanthophyll pigments | |
| CN102037130A (en) | Process for producing oils comprising PUFAs using microorganisms of the labyrinthulomycota order | |
| JP2002125601A (en) | Feed for animal plankton, method for producing the same, and method for culturing animal plankton | |
| JPH04248982A (en) | Production of bacteria capable of photosynthesis containing highly unsaturated fatty acid | |
| US8652814B2 (en) | Method for production of DHA-containing phospholipid through microbial fermentation | |
| JPH02171127A (en) | Bait for fish and shellfish | |
| JPH0286789A (en) | Production of fats and oils containing arachidonic acid by microorganism | |
| JPH04341180A (en) | Lactic acid bacterium containing highly unsaturated fatty acids and its production thereof | |
| JPH0216989A (en) | Production of omega6-based unsaturated fatty acid-containing phospholipid | |
| JP2001245687A (en) | Lipid containing n-4 and/or n-7 highly unsaturated fatty acid and method for producing the lipid | |
| JPH07147907A (en) | Live feed for fry and production thereof | |
| Izaara et al. | Effect of type of nutrient media on the biomass and fatty acid profiles of microalgae (Chlorella spp.) | |
| TWI844072B (en) | A novel schizochytrium sp. strain with high intracellular oil content and method for producing oil containing omega-3 using thereof | |
| JPH08163990A (en) | Oil-and-fat-containing alga and production of oil-and-fat derived therefrom | |
| Fisher Jr | Microscopic algae as industrial raw materials | |
| JPH0349688A (en) | Preparation of dihomo-gamma-linolenic acid and agent for inhibiting delta5-position unsaturation reaction of fatty acid | |
| JPH05123176A (en) | Production of highly unsaturated fatty acid phospholipid |