JPH03107746A - Vessel for detecting electrochemical emission - Google Patents
Vessel for detecting electrochemical emissionInfo
- Publication number
- JPH03107746A JPH03107746A JP24345089A JP24345089A JPH03107746A JP H03107746 A JPH03107746 A JP H03107746A JP 24345089 A JP24345089 A JP 24345089A JP 24345089 A JP24345089 A JP 24345089A JP H03107746 A JPH03107746 A JP H03107746A
- Authority
- JP
- Japan
- Prior art keywords
- flat plate
- light
- soln
- vessel
- detected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 125000006850 spacer group Chemical group 0.000 claims description 8
- 238000001378 electrochemiluminescence detection Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 19
- 230000003287 optical effect Effects 0.000 abstract description 6
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 239000012085 test solution Substances 0.000 description 20
- 239000000126 substance Substances 0.000 description 8
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 239000004593 Epoxy Substances 0.000 description 2
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 229910052731 fluorine Inorganic materials 0.000 description 2
- 239000011737 fluorine Substances 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- BBEAQIROQSPTKN-UHFFFAOYSA-N pyrene Chemical compound C1=CC=C2C=CC3=CC=CC4=CC=C1C2=C43 BBEAQIROQSPTKN-UHFFFAOYSA-N 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthrene Natural products C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- YYMBJDOZVAITBP-UHFFFAOYSA-N rubrene Chemical compound C1=CC=CC=C1C(C1=C(C=2C=CC=CC=2)C2=CC=CC=C2C(C=2C=CC=CC=2)=C11)=C(C=CC=C2)C2=C1C1=CC=CC=C1 YYMBJDOZVAITBP-UHFFFAOYSA-N 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
この発明は、被検溶液を絶縁性平板と光透過性平板との
間隙で保持して、被検溶液の所要量を少なくしたととも
に、光路長を短くして、検出感度の向上を図った電気化
学発光検出用容器に関するものである。[Detailed Description of the Invention] [Field of Industrial Application] This invention reduces the amount of test solution required by holding the test solution in the gap between an insulating flat plate and a light-transmitting flat plate, and also reduces the amount of test solution required for the optical path. This invention relates to an electrochemiluminescence detection container whose length is shortened to improve detection sensitivity.
抗原抗体反応における、抗原に固定された発光性物質の
発光が、抑制される現象を利用して、その時の発光量の
測定による種々の抗体の定量的測定等、電気化学発光検
出に用いられる検出用容器１として、第２図に示すよう
なものがある。すなわち、発光性物質を包含した被検溶
液２の入る筒状容器３の底壁３ａ内面の部分に、面電極
であって表面が反射鏡面である作用電極ＷＥを形成する
とともに、作用電極ＷＥと対峙する筒状容器３の内周面
３ｂの部分に、作用電極ＷＥと一定の間隔Ｇをもって、
一部にスリット部分Ｓを有するほぼ帯環状の面電極であ
って表面が反射鏡面である対電極ＣＥを形成して成るも
のである。そして、作用電極ＷＥには、対電極ＣＥのス
リット部分Ｓを通って筒状容器３の上端縁３Ｃに至る細
長いリード部分ＷＥ−１が形成され、対電極ＣＥには、
作用電極ＷＥ側のリード部分ＷＥ−１に平行して筒状容
器３の上端縁３Ｃに至る細長いリード部分ＣＥ−１が形
成されている。Detection used in electrochemiluminescence detection, such as quantitative measurement of various antibodies by measuring the amount of light emitted at that time, by making use of the phenomenon in which the light emission of a luminescent substance immobilized on an antigen is suppressed in an antigen-antibody reaction. There is a container 1 for use as shown in FIG. That is, the working electrode WE, which is a plane electrode and has a reflective mirror surface, is formed on the inner surface of the bottom wall 3a of the cylindrical container 3 containing the test solution 2 containing a luminescent substance. At a portion of the inner circumferential surface 3b of the cylindrical container 3 facing each other, with a certain distance G from the working electrode WE,
The counter electrode CE is a substantially ring-shaped surface electrode having a slit portion S in a part thereof, and a counter electrode CE whose surface is a reflective mirror surface. The working electrode WE is formed with an elongated lead portion WE-1 that passes through the slit portion S of the counter electrode CE and reaches the upper edge 3C of the cylindrical container 3.
An elongated lead portion CE-1 extending to the upper edge 3C of the cylindrical container 3 is formed parallel to the lead portion WE-1 on the working electrode WE side.
検出用容器１を用いて電気化学発光の検出を行うには、
リード部分ＷＥ−１、ＣＥ−１にクリップ等の接続手段
４を介してパルス電圧発生器５（例えば、ファンクシラ
ン・ジェネレータとボテンシオスタットから構成されて
いる。）を接続し、また、検出用容器ｌの上方開口の直
上に、検出用容器１内からの光りを受光し、受光量の強
弱をグラフまたは数字等で表示する光検出装置６（例え
ば、フォトマルチプライヤ等の光検出器と、プリアンプ
と、パルスカウントと、オシログラフ等の信号出力器か
ら構成されている。）を設置する。To detect electrochemiluminescence using the detection container 1,
A pulse voltage generator 5 (for example, composed of a funxilane generator and a botensiostat) is connected to the lead portions WE-1 and CE-1 via connection means 4 such as clips, and a detection Directly above the upper opening of the container l, a photodetector device 6 (for example, a photodetector such as a photomultiplier) that receives light from inside the detection container 1 and displays the strength of the received light amount in a graph or numbers, etc. It consists of a preamplifier, a pulse counter, and a signal output device such as an oscilloscope).
そして、被検溶液２をその液面が対電極ＣＥを越える位
置まで検出用容器１内に入れ、パルス電圧発生器５を操
作して、作用電極ＷＥと対電極ＧＥの間に所定のパルス
電圧を印加する。パルス電圧の印加によって被検溶液２
中に含まれている発光性物質が発光し、その発光量は光
検出装置６で所定単位に換算され、グラフまたは数字等
で表示される。Then, the test solution 2 is put into the detection container 1 until the liquid level exceeds the counter electrode CE, and the pulse voltage generator 5 is operated to generate a predetermined pulse voltage between the working electrode WE and the counter electrode GE. Apply. Test solution 2 is removed by applying a pulse voltage.
The luminescent substance contained therein emits light, and the amount of light emitted is converted into a predetermined unit by the photodetector 6 and displayed as a graph or number.
なお、電気化学発光検出用容器１で使用される被検溶液
２は、抗原抗体反応が行われる、緩衝液を含有する水溶
液を含むが、その抗原としては、各種の蛋白質、ポリペ
プチド、多糖体、脂質、核酸等を対象とすることができ
、その発光性物質としては、ルミノール、ピレン、アン
トラセン、ルブレンおよびこれらの誘導体等を用いるこ
とができる。また、抗原への発光性物質の固定は、通常
の架橋剤を用いて行う。The test solution 2 used in the electrochemiluminescence detection container 1 includes an aqueous solution containing a buffer solution in which an antigen-antibody reaction is performed, and the antigen includes various proteins, polypeptides, and polysaccharides. , lipids, nucleic acids, etc., and luminescent substances such as luminol, pyrene, anthracene, rubrene, and derivatives thereof can be used. Furthermore, the luminescent substance is immobilized on the antigen using a common crosslinking agent.
この従来技術においては、検出用容器１は、筒状容器３
であって、その底壁３ａ内面と内周面３ｂとで被検溶液
２を保持している。それゆえ、被検溶液２を多く用意し
なければならないという課題がある。また、この従来技
術においては、作用電極ＷＥの近傍で生じた発光しは、
検出用容器１内の被検溶液２の中を通って光検出装？！
！６に達するが、作用電極ＷＥの近傍から光検出装ｙ１
６までの距離が長い、それゆえ、光検出装置６の受光面
での受光量（照度）は、光源たる発光性物質までの距離
の二乗に逆比例するために、少ない。したがって、この
従来技術においては、検出感度が十分ではないという課
題がある。In this prior art, the detection container 1 includes a cylindrical container 3
The test solution 2 is held between the inner surface of the bottom wall 3a and the inner peripheral surface 3b. Therefore, there is a problem that a large amount of the test solution 2 must be prepared. Furthermore, in this prior art, the light emitted near the working electrode WE is
The photodetector passes through the test solution 2 in the detection container 1? !
! 6, but the photodetector y1 is detected from the vicinity of the working electrode WE.
Therefore, the amount of light received (illuminance) on the light-receiving surface of the photodetector 6 is small because it is inversely proportional to the square of the distance to the light-emitting substance that is the light source. Therefore, this conventional technique has a problem in that the detection sensitivity is not sufficient.
この発明は、このような従来技術の課題を解決する目的
でなされたものである。This invention was made with the aim of solving the problems of the prior art.
〔課題を解決するための手段］
上記課題を解決するための手段を、実施例に対応する第
１図を用いて以下、説明する。この発明は、縦方向の一
対のスペーサ１１を介して絶縁性平板１２と光透過性平
板１３とが間隙をもって対向、固定されているとともに
、絶縁性平板１２の対向面に作用電極ＷＥと対電極ＣＥ
とが間隙をもって縦方向に配置されているものである。[Means for Solving the Problems] Means for solving the above problems will be explained below using FIG. 1 corresponding to the embodiment. In this invention, an insulating flat plate 12 and a light-transmitting flat plate 13 are opposed to each other with a gap therebetween and are fixed via a pair of vertical spacers 11, and a working electrode WE and a counter electrode are provided on opposing surfaces of the insulating flat plate 12. C.E.
are arranged vertically with a gap between them.
このように構成されたものにおいては、被検溶液は、絶
縁性平板１２と光透過性平板１３との間隙で保持される
。また、作用電極ＷＥの近傍で生じた発光は、光透過性
平板１３を通って、少ない光路長さで光検出装置６に達
する。In the structure configured in this way, the test solution is held in the gap between the insulating flat plate 12 and the light-transmitting flat plate 13. Furthermore, the light emitted near the working electrode WE passes through the light-transmitting flat plate 13 and reaches the photodetector 6 with a short optical path length.
［実施例〕 第１図はこの発明の一実施例を示す図である。[Example〕 FIG. 1 is a diagram showing an embodiment of the present invention.
第１図において、１１は縦方向の一対のスペーサ、１２
は絶縁性平板、１３は光透過性平板、ＷＥは作用電極、
ＣＥは対電極である。スペーサ１１は、アクリル・スチ
レン・塩化ビニル・シリコン・エポキシ・ふっ素各樹脂
等のものが用いられ、その大きさは、例えば、幅１．０
×高さ２０．ＯＸ厚さ０．１ｍｍである。In FIG. 1, 11 is a pair of vertical spacers, 12
is an insulating flat plate, 13 is a transparent flat plate, WE is a working electrode,
CE is the counter electrode. The spacer 11 is made of acrylic, styrene, vinyl chloride, silicone, epoxy, fluorine resin, etc., and its size is, for example, 1.0 mm wide.
×Height 20. The OX thickness is 0.1 mm.
絶縁性平板１２は、アクリル・スチレン・エポキシ・ふ
っ素各樹脂等のものが用いられ、その大きさは、例えば
、輻１５．ＯＸ高さ２０．ＯＸ厚さ３．０ｍである。The insulating flat plate 12 is made of acrylic, styrene, epoxy, fluorine resin, etc., and its size is, for example, 15 mm. OX height 20. The OX thickness is 3.0 m.
光透過性平板１３は、発光波長域において光透過性の高
い材質が好ましく、発光物質にもよるが、例えば、可視
光域の発光物質では、ガラスやアクリル樹脂等が用いら
れ、その大きさは、例えば、幅１５．ＯＸ高さ２０．Ｏ
Ｘ厚さ１．Ｏｍｍである。The light-transmitting flat plate 13 is preferably made of a material with high light transmittance in the emission wavelength range, and it depends on the luminescent substance. For example, for luminescent substances in the visible light range, glass, acrylic resin, etc. are used, and the size , for example, width 15. OX height 20. O
X thickness 1. It is Omm.
作用電極ＷＥは、光透過性平板１３と対向する絶縁性平
板１２の対向面に形成された、導電性材料、好ましくは
白金、金、銀等の酸化還元電位の高い金属の蒸着厚膜、
スパッタ膜、印刷膜または仮であって、その大きさは、
例えば、幅１Ｏ８０■×高さ１０．ＯｎｍＸ厚さ５０μ
ｍである。作用電極ＷＥの一部には、絶縁性平板１２の
側面から突出され、かつ、クリップ等の接続手段が接続
されるリード部分ＷＥ−１が形成されている。The working electrode WE is a vapor-deposited thick film of a conductive material, preferably a metal with a high redox potential such as platinum, gold, or silver, formed on the opposite surface of the insulating flat plate 12 facing the light-transmitting flat plate 13.
Sputtered film, printed film or temporary film, the size of which is:
For example, width 1080cm x height 10. OnmX thickness 50μ
It is m. A lead portion WE-1 is formed in a part of the working electrode WE, which projects from the side surface of the insulating flat plate 12 and is connected to a connecting means such as a clip.
対電極ＣＥは、光透過性平板１３と対向する絶縁性平板
１２の対向面に、作用電極ＷＥと一定の間隔をもって形
成された、導電性材料、好ましくは白金、金、銀等の酸
化還元電位の高い金属の蒸着厚膜、スパッタ膜、印刷膜
または板であって、その大きさは、例えば、幅１０．ｏ
ｍｍＸ高さ６、ＯｓＸ厚さ５０μｍである。対電極ＣＥ
の一部には、絶縁性平板１２の側面から突出され、かつ
、クリップ等の接続手段が接続されるリード部分ＣＥ−
１が形成されている。作用電極ＷＥまたは対電極ＣＥは
、面電極である。The counter electrode CE is made of a conductive material, preferably platinum, gold, silver, etc., and is formed on the opposite surface of the insulating flat plate 12 facing the light-transmitting flat plate 13 at a constant distance from the working electrode WE. A vapor-deposited thick film, sputtered film, printed film or plate of a metal with a high thickness, for example, a width of 10. o
The dimensions are mm x height 6, and Os x thickness 50 μm. Counter electrode CE
A lead portion CE- protrudes from the side surface of the insulating flat plate 12 and is connected to a connecting means such as a clip.
1 is formed. The working electrode WE or the counter electrode CE is a plane electrode.
スペーサ１１によって、絶縁性平板１２と光透過性平板
１３とは一定間隔をもって一体となり、検出用容器ｌＯ
が形成される。なお、スペーサ１１の一方は、絶縁性で
あって、リード部分ＷＥ−１，ＣＥ−１に対応する凹部
１１Ａが形成されている。Due to the spacer 11, the insulating flat plate 12 and the light-transmitting flat plate 13 are integrated with a certain interval, and the detection container lO
is formed. Note that one of the spacers 11 is insulative and has a recess 11A formed therein corresponding to the lead portions WE-1 and CE-1.
検出用容器１０は、上部開口部１０Ａと下部開口部１０
Ｂを有する平行容器である。The detection container 10 has an upper opening 10A and a lower opening 10.
A parallel container with B.
検出用容器１０に被検溶液を供給するには、被検溶液が
入っている容器に検出用容器１０を立てると、絶縁性平
板１２と光透過性平板１３との間隙が狭いために、毛管
現象が生じ、これによって被検溶液は、下部開口部１０
Ｂから絶縁性平板１２と光透過性平板１３との間隙を上
がる。したがって間隙の狭い平行容器であっても、被検
溶液の供給に支障は生じない。そして、被検溶液が入っ
ている容器から検出用容器ｌＯを引き上げるが、表面張
力によって、被検溶液は絶縁性平板１２と光透過性平板
１３との間に保持されている。To supply the test solution to the detection container 10, when the detection container 10 is placed upright in the container containing the test solution, the gap between the insulating flat plate 12 and the light-transmitting flat plate 13 is narrow, so the capillary phenomenon occurs, whereby the test solution flows through the lower opening 10.
From B, go up the gap between the insulating flat plate 12 and the light-transmitting flat plate 13. Therefore, even if the parallel containers have narrow gaps, there will be no problem in supplying the test solution. Then, the detection container IO is pulled up from the container containing the test solution, but the test solution is held between the insulating flat plate 12 and the light-transmitting flat plate 13 due to surface tension.
検出用容器ｌＯは、筒状容器ではなく、間隙の狭い平行
容器であるため、被検溶液の所要量は少なくて済む。Since the detection container IO is not a cylindrical container but a parallel container with a narrow gap, the required amount of the test solution is small.
作用電極ＷＥの近傍で生じた発光は、光透過性平板１３
を通って、少ない光路長さで光検出装置６に達する。光
透過性平板１３側に障害物はないので、光透過性平板１
３と光検出装置６とは極めて接近できる。それゆえ、光
検出装置６の受光面での受光量（照度）が増大し、その
検出感度は向上する。The light emitted near the working electrode WE is transmitted through the light-transmitting flat plate 13.
and reaches the photodetector device 6 with a short optical path length. Since there are no obstacles on the side of the light-transmitting flat plate 13, the light-transmitting flat plate 1
3 and the photodetector 6 can be very close to each other. Therefore, the amount of light received (illuminance) on the light receiving surface of the photodetector 6 increases, and its detection sensitivity improves.
以上説明してきたように、この発明は、縦方向の一対の
スペーサを介して絶縁性平板と光透過性平板とが間隙を
もって対向、固定されているとともに、該絶縁性平板の
対向面に作用電極と対電極とが間隙をもって縦方向に配
置されているものである。それゆえ、被検溶液は絶縁性
平板と光透過性平板との間隙で保持され、また、光路長
は短い。As explained above, in the present invention, an insulating flat plate and a light-transmitting flat plate are faced and fixed with a gap through a pair of vertical spacers, and a working electrode is provided on the opposing surface of the insulating flat plate. and a counter electrode are arranged vertically with a gap between them. Therefore, the test solution is held in the gap between the insulating flat plate and the light-transmitting flat plate, and the optical path length is short.
したがって、この発明によれば、被検溶液の所要量は少
なくて済むとともに、検出感度は向上するという効果が
得られる。Therefore, according to the present invention, the required amount of the test solution can be reduced and the detection sensitivity can be improved.
第１図はこの発明の一実施例を示す図で、（ａ）は平面
図、（ｂ）は断面図、（ｃ）は斜視図、第２図は従来の
技術を示す図で、（ａ）は断面図、（ｂ）は斜視概略図
である。
１１・・・スペーサ、１２・・・絶縁性平板、１３・・
・光透過性平板、ＷＥ・・・作用電極、ＣＥ・・・対電
極箱
図
（（１）
（Ｃ）
（ｂ）
ＣＥ−１FIG. 1 is a diagram showing an embodiment of the present invention, (a) is a plan view, (b) is a sectional view, (c) is a perspective view, and FIG. 2 is a diagram showing a conventional technique. ) is a sectional view, and (b) is a schematic perspective view. 11... Spacer, 12... Insulating flat plate, 13...
・Light-transparent flat plate, WE...working electrode, CE...counter electrode box diagram ((1) (C) (b) CE-1
Claims (1)
過性平板とが間隙をもって対向、固定されているととも
に、該絶縁性平板の対向面に作用電極と対電極とが間隙
をもって縦方向に配置されている電気化学発光検出用容
器1. An insulating flat plate and a light-transmitting flat plate are opposed and fixed with a gap between them via a pair of spacers in the vertical direction, and a working electrode and a counter electrode are arranged in the vertical direction with a gap on the opposing surfaces of the insulating flat plate. Electrochemiluminescence detection container located in
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24345089A JPH03107746A (en) | 1989-09-21 | 1989-09-21 | Vessel for detecting electrochemical emission |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24345089A JPH03107746A (en) | 1989-09-21 | 1989-09-21 | Vessel for detecting electrochemical emission |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03107746A true JPH03107746A (en) | 1991-05-08 |
Family
ID=17104065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24345089A Pending JPH03107746A (en) | 1989-09-21 | 1989-09-21 | Vessel for detecting electrochemical emission |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03107746A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992015009A1 (en) * | 1991-02-19 | 1992-09-03 | Tdk Corporation | Method of analyzing electrochemiluminescent substance and apparatus therefor |
| US5240863A (en) * | 1991-02-19 | 1993-08-31 | Tdk Corporation | Method of measuring immunoreactant using electrochemiluminescence |
-
1989
- 1989-09-21 JP JP24345089A patent/JPH03107746A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992015009A1 (en) * | 1991-02-19 | 1992-09-03 | Tdk Corporation | Method of analyzing electrochemiluminescent substance and apparatus therefor |
| US5240863A (en) * | 1991-02-19 | 1993-08-31 | Tdk Corporation | Method of measuring immunoreactant using electrochemiluminescence |
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