JP2869866B2 - Container for electrochemiluminescence detection - Google Patents
Container for electrochemiluminescence detectionInfo
- Publication number
- JP2869866B2 JP2869866B2 JP1260073A JP26007389A JP2869866B2 JP 2869866 B2 JP2869866 B2 JP 2869866B2 JP 1260073 A JP1260073 A JP 1260073A JP 26007389 A JP26007389 A JP 26007389A JP 2869866 B2 JP2869866 B2 JP 2869866B2
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- JP
- Japan
- Prior art keywords
- insulating
- horizontally
- fixed
- electrodes
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Description
【発明の詳細な説明】 〔産業上の利用分野〕 この発明は、単体ではなく、多数体にして、多項目の
同時、順次検査等を容易にした電気化学発光検出用容器
に関するものである。Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a container for electrochemical chemiluminescence detection, which is not a single substance but a large number of which facilitates simultaneous and sequential inspection of multiple items.
抗原抗体反応における、抗原に固定された発光性物質
の発光が、抑制される現象を利用して、その時の発光量
の測定による種々の抗体の定量的測定等、電気化学発光
検出に用いられる検出用容器１として、第４図に示すよ
うなものがある。すなわち、発光性物質を包含した被検
溶液２の入る筒状容器３の底壁3a内面の部分に、面電極
であって表面が反射鏡面である作用電極WEを形成すると
ともに、筒状容器３の内周面3bの部分に、作用電極WEと
一定の間隔Ｇをもつて対峙する、一部にスリット部分Ｓ
を有するほぼ帯環状の面電極であって表面が反射鏡面で
ある対電極CEを形成して成るものである。そして、作用
電極WEには、対電極CEのスリット部分Ｓを通って筒状容
器３の上端縁3cに至る細長いリード部分WE−１が形成さ
れ、対電極CEには、作用電極WE側のリード部分WE−１に
平行して筒状容器３の上端縁3cに至る細長いリード部分
CE−１が形成されている。Utilizing the phenomenon that the emission of a luminescent substance immobilized on an antigen is suppressed in an antigen-antibody reaction, the detection used for electrochemical chemiluminescence detection, such as quantitative measurement of various antibodies by measuring the amount of luminescence at that time FIG. 4 shows an example of the container 1 for use. That is, a working electrode WE which is a surface electrode and whose surface is a reflecting mirror surface is formed on the inner surface of the bottom wall 3a of the cylindrical container 3 into which the test solution 2 containing the luminescent substance enters, and the cylindrical container 3 Is opposed to the working electrode WE at a constant interval G at a portion of the inner peripheral surface 3b, and a portion of the slit portion S
And a counter electrode CE whose surface is a reflecting mirror surface. The working electrode WE is formed with an elongated lead portion WE-1 which reaches the upper edge 3c of the cylindrical container 3 through the slit portion S of the counter electrode CE, and the counter electrode CE has a lead on the side of the working electrode WE. An elongated lead portion that reaches the upper edge 3c of the cylindrical container 3 in parallel with the portion WE-1
CE-1 is formed.
検出用容器１を用いて電気化学発光の検出を行うに
は、リード部分WE−１、CE−１にクリップ等の接続手段
４を介してパルス電圧発生器５（例えば、ファンクショ
ン・ジェネレータとポテンシオスタットから構成されて
いる。）を接続し、また、検出用容器１の上方開口の直
上に、検出用容器１内からの光Ｌを受光し、受光量の強
弱をグラフまたは数字等で表示する光検出装置６（例え
ば、フォトマルチプライヤ等の光検出器と、プリアンプ
と、パルスカウンタと、オシログラフ等の信号出力器か
ら構成されている。）を設置する。そして、被検溶液２
をその液面が対電極CEを越える位置まで検出用容器１内
に入れ、パルス電圧発生器５を操作して、作用電極WEと
対電極CEの間に所定のパルス電圧を印加する。パルス電
圧の印加によって被検溶液２中に含まれている発光性物
質が発光し、その発光量は光検出装置６で所定単位に換
算され、グラフまたは数字等で表示される。In order to detect the electrochemiluminescence using the detection container 1, a pulse voltage generator 5 (for example, a function generator and a potentiometer) is connected to the lead portions WE-1 and CE-1 via connecting means 4 such as a clip. The light L from the inside of the detection container 1 is received just above the upper opening of the detection container 1, and the intensity of the received light is indicated by a graph or a number. A photodetector 6 (for example, a photodetector such as a photomultiplier, a preamplifier, a pulse counter, and a signal output device such as an oscillograph) is installed. And the test solution 2
Is put into the detection container 1 until the liquid level exceeds the counter electrode CE, and the pulse voltage generator 5 is operated to apply a predetermined pulse voltage between the working electrode WE and the counter electrode CE. The light-emitting substance contained in the test solution 2 emits light by application of the pulse voltage, and the amount of the emitted light is converted into a predetermined unit by the light detection device 6 and is displayed as a graph or a number.
なお、電気化学発光検出用容器１で使用される被検溶
液２は、抗原抗体反応が行われる、緩衝液を含有する水
溶液を含むが、その抗原としては、各種の蛋白質、ポリ
ペプチド、多糖体、脂質、核酸等を対象とすることがで
き、その発光性物質としては、ルミノール、ピレン、ア
ントラセン、ルブレンおよびこれらの誘導体等を用いる
ことができる。また、抗原への発光性物質の固定は、通
常の架橋剤を用いて行う。The test solution 2 used in the container 1 for electrochemiluminescence detection contains an aqueous solution containing a buffer solution in which an antigen-antibody reaction is performed. Examples of the antigen include various proteins, polypeptides, and polysaccharides. , Lipids, nucleic acids, and the like, and as the luminescent substance, luminol, pyrene, anthracene, rubrene, derivatives thereof, and the like can be used. Further, the luminescent substance is fixed to the antigen using a usual crosslinking agent.
この従来技術においては、検出用容器１は、単体であ
る。それゆえ、一人に関して多項目を同時、順次に検査
し、または多数人に関して同一項目を同時、順次に検査
する場合には、検出用容器１を順次に使用するか、また
は検出用容器１を複数、集合しなければならない。した
がって、かかる場合、その取扱は不便であるという課題
がある。In this prior art, the detection container 1 is a single unit. Therefore, when multiple items are inspected simultaneously and sequentially for one person, or when the same item is inspected simultaneously and sequentially for many people, the detection containers 1 are used sequentially or a plurality of the detection containers 1 are used. , Must gather. Therefore, in such a case, there is a problem that the handling is inconvenient.
この発明は、このような従来技術の課題を解決する目
的でなされたものである。The present invention has been made for the purpose of solving such problems of the prior art.
上記課題を解決するための手段を、実施例に対応する
第１図を用いて以下、説明する。この発明は、絶縁性平
板11上に、連結部CE−１を介して複数の対電極CEが電気
的に接続されたものの複数、配置により各セル12の対電
極CEが縦横に固定されているとともに、各セル12の内壁
下部を構成する孔12Aが縦横に穿設された下部絶縁性有
孔板13が固定され、下部絶縁性有孔板13上に、連結部WE
−１を介して対電極CEより対向面積が小さい、複数の作
用電極WEが電気的に接続されたものの複数、配置により
作用電極WEが対電極CEと対向して縦横に固定されている
とともに、各セル12の内壁上部を構成する孔12Bが縦横
に穿設された上部絶縁性有孔板14が固定されているもの
である。The means for solving the above problems will be described below with reference to FIG. 1 corresponding to the embodiment. According to the present invention, a plurality of counter electrodes CE are electrically connected to each other on the insulating flat plate 11 via a connecting portion CE-1. At the same time, a lower insulating perforated plate 13 in which holes 12A forming the lower portion of the inner wall of each cell 12 are formed vertically and horizontally is fixed, and the connecting portion WE is placed on the lower insulating perforated plate 13.
−1, the facing area is smaller than the counter electrode CE, the plurality of working electrodes WE are electrically connected. An upper insulating perforated plate 14 in which holes 12B forming the upper part of the inner wall of each cell 12 are formed vertically and horizontally is fixed.
このように構成されたものにおいては、検出用容器10
は、単体ではなく、多数体である。In such a configuration, the detection container 10
Is not a simple substance but a multiple body.
第１図はこの発明の一実施例を示す図である。第１図
において、11は絶縁性平板、12はセル、13は下部絶縁性
有孔板、14は上部絶縁性有孔板、CEは対電極、WEは作用
電極である。絶縁性平板11は、スチレン・アクリル・エ
ポキシ・ふっ素各樹脂、ポリカーボネードなどのものが
用いられ、その大きさは、例えば、幅86.0×高さ128.2
×厚さ5.0mmである。絶縁性平板11上には、連結部CE−
１を介して複数の対電極CEが電気的に接続されたものが
複数、固定されている。連結部CE−１、対電極CEは、導
電性材料、好ましくは白金、金、銀等の酸化還元電位の
高い金属の蒸着厚膜、スパッタ膜、印刷膜または板であ
って、対電極CEの形状は、例えば直径8mmの円である。
この複数、固定によって、セル12の対電極CEは縦横に配
置される。絶縁性平板11上に下部絶縁性有孔板13が固定
されている。下部絶縁性有孔板13には、セル12の内壁下
部を構成する孔12Aが縦横に穿設されている。下部絶縁
性有孔板13の下面には、連結部CE−１に対応する凹部13
Aが形成されている。孔12Aは、対電極CEと同じ大きさに
形成されている。また、下部絶縁性有孔板13の上面に
は、連結部WE−１に対応する凹部13Bが形成されてい
る。下部絶縁性有孔板13上には、連結部WE−１を介して
複数の作用電極WEが電気的に接続されたものが複数、凹
部13Bに固定されている。作用電極WEは、対電極CEより
対向面積が小さく、対電極CEと対向している。連結部WE
−１、作用電極WEは、導電性材料、好ましくは白金、
金、銀等の酸化還元電位の高い金属の板であって、作用
電極WEの形状は、例えば、直径5mmの円である。この複
数、固定によって、セル12の作用電極WEは縦横に配置さ
れる。下部絶縁性有孔板13上に上部絶縁性有孔板14が固
定されている。上部絶縁性有孔板14には、セル12の内壁
上部を構成する孔12Bが縦横に穿設されている。孔12B
は、孔12Aと同じ大きさに形成されている。作用電極WE
および対電極CEは、面電極であって表面が反射鏡面であ
る。FIG. 1 shows an embodiment of the present invention. In FIG. 1, 11 is an insulating flat plate, 12 is a cell, 13 is a lower insulating perforated plate, 14 is an upper insulating perforated plate, CE is a counter electrode, and WE is a working electrode. The insulating plate 11 is made of styrene, acrylic, epoxy, fluorine resin, polycarbonate, or the like, and its size is, for example, width 86.0 × height 128.2.
× The thickness is 5.0 mm. The connecting part CE-
A plurality of counter electrodes CE electrically connected to each other via 1 are fixed. The connection part CE-1 and the counter electrode CE are a conductive material, preferably a deposited thick film of a metal having a high oxidation-reduction potential such as platinum, gold, and silver, a sputtered film, a printed film or a plate. The shape is, for example, a circle having a diameter of 8 mm.
By this plurality and fixing, the counter electrodes CE of the cell 12 are arranged vertically and horizontally. The lower insulating perforated plate 13 is fixed on the insulating flat plate 11. In the lower insulating perforated plate 13, holes 12A forming the lower part of the inner wall of the cell 12 are formed vertically and horizontally. On the lower surface of the lower insulating perforated plate 13, a concave portion 13 corresponding to the connecting portion CE-1 is provided.
A is formed. The hole 12A is formed in the same size as the counter electrode CE. In addition, a concave portion 13B corresponding to the connecting portion WE-1 is formed on the upper surface of the lower insulating perforated plate 13. On the lower insulating perforated plate 13, a plurality of working electrodes WE electrically connected to each other via the connecting portion WE-1 are fixed to the concave portion 13B. The working electrode WE has a smaller facing area than the counter electrode CE, and faces the counter electrode CE. Connection WE
-1, the working electrode WE is a conductive material, preferably platinum,
It is a metal plate having a high oxidation-reduction potential such as gold or silver, and the shape of the working electrode WE is, for example, a circle having a diameter of 5 mm. Due to the plurality and fixing, the working electrodes WE of the cell 12 are arranged vertically and horizontally. An upper insulating perforated plate 14 is fixed on the lower insulating perforated plate 13. In the upper insulating perforated plate 14, holes 12B constituting the upper part of the inner wall of the cell 12 are formed vertically and horizontally. Hole 12B
Is formed in the same size as the hole 12A. Working electrode WE
The counter electrode CE is a plane electrode and has a reflective mirror surface.
被検溶液は、作用電極WEとセル12の内壁下部12Aとの
間隙から、対電極CEと作用電極WEとの間に供給される。
また、この間隙から、作用電極WEの近傍で生じる発光
は、光検出装置（図示せず）に達する。The test solution is supplied between the counter electrode CE and the working electrode WE from the gap between the working electrode WE and the lower portion 12A of the inner wall of the cell 12.
In addition, light emitted near the working electrode WE from this gap reaches a photodetector (not shown).
作用電極WEの形状は、円形ではなく、第３図（ａ）
（ｂ）に示すように、ワッシャ状、パイプ状に形成して
もよい。この場合、その外径は、孔12Bと略等しく、中
央の孔から、被検溶液は供給され、発光は光検出装置に
達する。The shape of the working electrode WE is not circular,
As shown in (b), it may be formed in a washer shape or a pipe shape. In this case, the outer diameter is substantially equal to the hole 12B, the test solution is supplied from the central hole, and the light emission reaches the photodetector.
以上は、絶縁性平板11上に固定されている下部絶縁性
有孔板13に固定、支持された連結部Ｗ−１によって、作
用電極WEと対電極CEとは、その間に介在するものがな
く、対向しているが、第２図に示すように、各セル12の
対電極CE上に、連結部WE−１を介して対電極CEより対向
面積が小さい、複数の作用電極WEが電気的に接続された
ものを複数、絶縁性スペーサ21を介して配置して、作用
電極WEを対電極CEに対向せしめ、絶縁性平板11上に、各
セル12の内壁を構成する孔12Cが縦横に配置されている
絶縁性有孔板22を固定してもよい。なお、図示しない
が、絶縁性有孔板22の下面には、絶縁性スペーサ21及び
連結部WE−１に対応する凹部が形成されている。In the above, the working electrode WE and the counter electrode CE are not interposed between the working electrode WE and the counter electrode CE by the connecting portion W-1 fixed and supported on the lower insulating perforated plate 13 fixed on the insulating flat plate 11. As shown in FIG. 2, a plurality of working electrodes WE having a smaller facing area than the counter electrode CE are electrically connected to the counter electrode CE of each cell 12 via the connecting portion WE-1, as shown in FIG. The working electrode WE is opposed to the counter electrode CE, and a plurality of holes 12C constituting the inner wall of each cell 12 are vertically and horizontally formed on the insulating flat plate 11. The arranged insulating perforated plate 22 may be fixed. Although not shown, a concave portion corresponding to the insulating spacer 21 and the connecting portion WE-1 is formed on the lower surface of the insulating perforated plate 22.
第２図（ａ）、（ｂ）において、絶縁性スペーサ21
は、角柱で、連結部CE−１を介して複数の対電極CEが電
気的に接続されたものと直交し、連続しているが、これ
らと平行し、連続していてもよい。また、絶縁性スペー
サ21は、製作上から連続しているが、断続していてもよ
い。さらに、絶縁性スペーサ21は、一条でなく、複数で
あってもよい。第２図（ｂ）に示すように、絶縁性スペ
ーサ21は角柱ではなく、円柱にしてもよい。作用電極WE
が第３図（ａ）（ｂ）に示すようなワッシャ状、パイプ
状である場合、絶縁性スペーサ21として、同じ形状のも
のが用いられてもよい。2 (a) and 2 (b), the insulating spacer 21
Is a prism and is orthogonal to and continuous with a plurality of counter electrodes CE electrically connected via the connecting portion CE-1, but may be parallel or continuous with these. Further, the insulating spacer 21 is continuous from the manufacturing point of view, but may be intermittent. Further, the number of the insulating spacers 21 is not limited to one, but may be plural. As shown in FIG. 2 (b), the insulating spacer 21 may be a cylinder instead of a prism. Working electrode WE
In the case where the insulating spacer 21 has a washer shape or a pipe shape as shown in FIGS. 3 (a) and 3 (b), the insulating spacer 21 having the same shape may be used.
このように、検出用容器10は、セル12の集合体であ
る。それゆえ、一人に関して多項目を同時、順次に検査
し、または多数人に関して同一項目を同時、順次に検査
する場合には、順次使用、複数集合を要せず、その取扱
が容易になる。As described above, the detection container 10 is an aggregate of the cells 12. Therefore, when multiple items are inspected simultaneously and sequentially for one person, or when the same item is inspected simultaneously and sequentially for many people, it is not necessary to use sequentially and a plurality of sets, and the handling becomes easy.
なお、検出用容器10を用いて電気化学発光の検出は、
絶縁性平板11に露呈している連結部WE−１、CE−１に、
クリップ等の接続手段を介してパルス電圧発生器（例え
ば、ファンクション・ジェネレータとポテンシオスタッ
トから構成されている。）を接続し、また、検出用容器
10の上方開口、すなわち、孔12Bの直上に、検出用容器1
0内からの光を受光し、受光量の強弱をグラフまたは数
字等で表示する光検出装置を配置して行う。この場合、
パルス電圧発生器、光検出装置も、検出用容器10と同様
にマトリクス状に構成すると、その取扱は一層、容易に
なる。In addition, the detection of electrochemiluminescence using the detection container 10,
The connecting portions WE-1 and CE-1 exposed on the insulating plate 11
A pulse voltage generator (for example, composed of a function generator and a potentiostat) is connected via connection means such as a clip, and a container for detection is connected.
In the upper opening of 10, that is, just above the hole 12B, the detection vessel 1
A light detection device that receives light from inside 0 and displays the intensity of the received light with a graph or a numeral is arranged. in this case,
If the pulse voltage generator and the photodetector are also arranged in a matrix like the detection container 10, the handling becomes even easier.
以上説明してきたように、この発明は、絶縁性平板上
に、連結部を介して複数の対電極が電気的に接続された
ものの複数、配置により各セルの対電極が縦横に固定さ
れているとともに、該各セルの内壁下部を構成する孔が
縦横に穿設された下部絶縁性有孔板が固定され、該下部
絶縁性有孔板上に、連結部を介して前記対電極より対向
面積が小さい、複数の作用電極が電気的に接続されたも
のの複数、配置により該作用電極が前記対電極と対向し
て縦横に固定されているとともに、前記各セルの内壁上
部を構成する孔が縦横に穿設された上部絶縁性有孔板が
固定されているもの、または、絶縁性平板上に、連結部
を介して複数の対電極が電気的に接続されたものの複
数、配置により各セルの対電極が縦横に固定され、該各
セルの対電極上に、連結部を介して該対電極より対向面
積が小さい、複数の作用電極が電気的に接続されたもの
の複数、絶縁性スペーサを介しての配置により該作用電
極が前記対電極に対向して配置され、前記絶縁性平板上
に、前記各セルの内壁を構成する孔が縦横に穿設された
絶縁性有孔板が固定されているものである。それゆえ、
検出用容器は、単体ではなく、多数体である。したがっ
て、この発明によれば、多項目の同時、順次検査等が容
易であるという効果が得られる。As described above, in the present invention, a plurality of counter electrodes are electrically connected to each other via a connecting portion on an insulating flat plate. At the same time, a lower insulating perforated plate in which holes constituting the lower portion of the inner wall of each cell are drilled vertically and horizontally is fixed. The plurality of working electrodes are electrically connected, but the plurality of working electrodes are fixed vertically and horizontally facing the counter electrode by arrangement, and the holes forming the upper part of the inner wall of each cell are vertically and horizontally. The upper insulated perforated plate perforated is fixed, or a plurality of counter electrodes are electrically connected via a connecting portion on an insulating flat plate. The counter electrode is fixed vertically and horizontally, and the cells are connected on the counter electrode of each cell. The working area is opposed to the counter electrode due to the arrangement of the plurality of working electrodes electrically connected to each other, although a plurality of working electrodes are electrically connected to each other through the insulating spacer. On the insulating flat plate, an insulating perforated plate having holes forming the inner wall of each of the cells is fixed vertically and horizontally. therefore,
The detection container is not a simple substance but a multiplicity. Therefore, according to the present invention, it is possible to obtain an effect that it is easy to simultaneously or sequentially inspect a plurality of items.
第１図はこの発明の一実施例を示す図で、（ａ）は平面
図、（ｂ）は斜視図、（ｃ）は断面図、（ｄ）は分解斜
視図、第２図はこの発明の他の実施例を示す図で、
（ａ）、（ｂ）は断面図、（ｃ）は分解斜視図、第３図
（ａ）、（ｂ）はこの発明を構成する作用電極の他の形
状を示す斜視図、第４図は従来の技術を示す図で、
（ａ）は断面図、（ｂ）は斜視概略図である。 11……絶縁性平板、12……セル、13……下部絶縁性有孔
板、14……上部絶縁性有孔板、22……絶縁性有孔板、21
……絶縁性スペーサ、WE……作用電極、CE……対電極FIG. 1 is a view showing one embodiment of the present invention, in which (a) is a plan view, (b) is a perspective view, (c) is a sectional view, (d) is an exploded perspective view, and FIG. FIG.
(A) and (b) are cross-sectional views, (c) is an exploded perspective view, FIGS. 3 (a) and 3 (b) are perspective views showing other shapes of a working electrode constituting the present invention, and FIG. In the diagram showing the conventional technology,
(A) is a sectional view, and (b) is a schematic perspective view. 11 ... insulated flat plate, 12 ... cell, 13 ... lower insulated perforated plate, 14 ... upper insulated perforated plate, 22 ... insulated perforated plate, 21
…… Insulating spacer, WE …… Working electrode, CE …… Counter electrode
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 昭56−6162（ＪＰ，Ａ) 特開 平３−107746（ＪＰ，Ａ) 特開 平３−107747（ＪＰ，Ａ) 特開 平３−107748（ＪＰ，Ａ) (58)調査した分野(Int.Cl.6，ＤＢ名) G01N 21/75 - 21/83 G01N 27/416 G01N 33/543 G01N 33/532 ──────────────────────────────────────────────────続 き Continued from the front page (56) References JP-A-56-6162 (JP, A) JP-A-3-107746 (JP, A) JP-A-3-107747 (JP, A) JP-A-3-107746 107748 (JP, A) (58) Field surveyed (Int. Cl. 6 , DB name) G01N 21/75-21/83 G01N 27/416 G01N 33/543 G01N 33/532
Claims (2)
電極が電気的に接続されたものの複数、配置により各セ
ルの対電極が縦横に固定されているとともに、該各セル
の内壁下部を構成する孔が縦横に穿設された下部絶縁性
有孔板が固定され、該下部絶縁性有孔板上に、連結部を
介して前記対電極より対向面積が小さい、複数の作用電
極が電気的に接続されたものの複数、配置により該作用
電極が前記対電極と対向して縦横に固定されているとと
もに、前記各セルの内壁上部を構成する孔が縦横に穿設
された上部絶縁性有孔板が固定されている電気化学発光
検出用容器A plurality of counter electrodes are electrically connected to each other via a connecting portion on an insulating flat plate, and the counter electrodes of each cell are fixed vertically and horizontally by a plurality of arrangements. A lower insulating perforated plate in which holes constituting the lower portion of the inner wall are perforated vertically and horizontally is fixed. A plurality of electrodes electrically connected to each other, and the working electrode is fixed vertically and horizontally facing the counter electrode by arrangement, and holes forming the upper inner wall of each cell are formed vertically and horizontally. Electrochemiluminescence detection container to which insulating perforated plate is fixed
電極が電気的に接続されたものの複数、配置により各セ
ルの対電極が縦横に固定され、該各セルの対電極上に、
連結部を介して該対電極より対向面積が小さい、複数の
作用電極が電気的に接続されたものの複数、絶縁性スペ
ーサを介しての配置により該作用電極が前記対電極に対
向して配置され、前記絶縁性平板上に、前記各セルの内
壁を構成する孔が縦横に穿設された絶縁性有孔板が固定
されている電気化学発光検出用容器2. A plurality of counter electrodes are electrically connected to each other via a connecting portion on an insulating flat plate. The counter electrodes of each cell are fixed vertically and horizontally by a plurality and arrangement of the counter electrodes. To
Although a plurality of working electrodes are electrically connected to each other with a smaller opposing area than the counter electrode via the connecting portion, the plurality of working electrodes are arranged to face the counter electrode by arrangement via an insulating spacer. An electrochemiluminescence detection container in which an insulating perforated plate in which holes forming the inner wall of each cell are formed vertically and horizontally is fixed on the insulating flat plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1260073A JP2869866B2 (en) | 1989-10-06 | 1989-10-06 | Container for electrochemiluminescence detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1260073A JP2869866B2 (en) | 1989-10-06 | 1989-10-06 | Container for electrochemiluminescence detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03122554A JPH03122554A (en) | 1991-05-24 |
| JP2869866B2 true JP2869866B2 (en) | 1999-03-10 |
Family
ID=17342930
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1260073A Expired - Fee Related JP2869866B2 (en) | 1989-10-06 | 1989-10-06 | Container for electrochemiluminescence detection |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2869866B2 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9406109D0 (en) * | 1994-03-28 | 1994-05-18 | Neotronics Ltd | Electrochemical sensor |
| HUP9801679A3 (en) * | 1995-03-10 | 2001-01-29 | Meso Scale Technologies Llc Co | Process and agent for multi-array, multi-specific electrochemiluminescence testing |
| JP3271547B2 (en) * | 1997-01-23 | 2002-04-02 | ダイキン工業株式会社 | Sensor device |
| JP2001133436A (en) * | 1999-11-01 | 2001-05-18 | Microtec Nition:Kk | Matrix type substrate for electrochemical emission |
| DE10028257A1 (en) * | 2000-06-09 | 2001-12-20 | Alpha Technology Ges Fuer Ange | Analysis chip used e.g. in DNA sequencing, comprises baseplate carrying array of electronically-addressable points, with matching perforated plate attached to it |
| JP4619529B2 (en) * | 2000-12-28 | 2011-01-26 | スターライト工業株式会社 | Test equipment |
| DE10102063A1 (en) * | 2001-01-17 | 2002-07-25 | Alpha Technology Ges Fuer Ange | Analysis chip, e.g. for genetic analysis, has layer comprising grid of electrically connected spots and second layer comprising conductive structure of rings connected by strips, where layers are separated by insulating layers |
| EP1546723A4 (en) * | 2002-08-16 | 2007-03-07 | Decision Biomarkers Inc | Reading of fluorescent arrays |
| US20060216203A1 (en) * | 2005-03-28 | 2006-09-28 | Mds Sciex (Us) A Division Of Mds Pharma Services (Us) Inc. | Multiwell sample plate with integrated impedance electrodes and connection scheme |
| JP5481326B2 (en) * | 2010-09-08 | 2014-04-23 | 株式会社日立製作所 | Measuring apparatus and measuring method using biomaterial detection element |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4280815A (en) * | 1979-06-18 | 1981-07-28 | Technicon Instruments Corporation | Electrochemiluminescent immunoassay and apparatus therefor |
-
1989
- 1989-10-06 JP JP1260073A patent/JP2869866B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03122554A (en) | 1991-05-24 |
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