JP7062667B2 - コラーゲンヒドロゲルの製造方法 - Google Patents
コラーゲンヒドロゲルの製造方法 Download PDFInfo
- Publication number
- JP7062667B2 JP7062667B2 JP2019533705A JP2019533705A JP7062667B2 JP 7062667 B2 JP7062667 B2 JP 7062667B2 JP 2019533705 A JP2019533705 A JP 2019533705A JP 2019533705 A JP2019533705 A JP 2019533705A JP 7062667 B2 JP7062667 B2 JP 7062667B2
- Authority
- JP
- Japan
- Prior art keywords
- collagen
- cells
- jellyfish
- collagen hydrogel
- purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000008186 Collagen Human genes 0.000 title claims description 183
- 108010035532 Collagen Proteins 0.000 title claims description 183
- 229920001436 collagen Polymers 0.000 title claims description 183
- 239000000017 hydrogel Substances 0.000 title claims description 108
- 241000242583 Scyphozoa Species 0.000 claims description 88
- 210000004027 cell Anatomy 0.000 claims description 34
- 238000004519 manufacturing process Methods 0.000 claims description 27
- 239000003431 cross linking reagent Substances 0.000 claims description 26
- AZKVWQKMDGGDSV-UHFFFAOYSA-N genipin Natural products COC(=O)C1=COC(O)C2C(CO)=CCC12 AZKVWQKMDGGDSV-UHFFFAOYSA-N 0.000 claims description 22
- AZKVWQKMDGGDSV-BCMRRPTOSA-N Genipin Chemical group COC(=O)C1=CO[C@@H](O)[C@@H]2C(CO)=CC[C@H]12 AZKVWQKMDGGDSV-BCMRRPTOSA-N 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 18
- 239000000872 buffer Substances 0.000 claims description 17
- 239000002253 acid Substances 0.000 claims description 15
- 230000015572 biosynthetic process Effects 0.000 claims description 14
- 238000006386 neutralization reaction Methods 0.000 claims description 14
- 238000000605 extraction Methods 0.000 claims description 13
- 210000001519 tissue Anatomy 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 12
- 238000004113 cell culture Methods 0.000 claims description 11
- 238000012604 3D cell culture Methods 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- 241000133526 Rhizostoma Species 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 210000002569 neuron Anatomy 0.000 claims description 5
- 241000146389 Rhopilema nomadica Species 0.000 claims description 4
- 210000002744 extracellular matrix Anatomy 0.000 claims description 4
- 210000003494 hepatocyte Anatomy 0.000 claims description 4
- 239000002674 ointment Substances 0.000 claims description 4
- 102000057297 Pepsin A Human genes 0.000 claims description 3
- 108090000284 Pepsin A Proteins 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- 238000012377 drug delivery Methods 0.000 claims description 3
- 210000002510 keratinocyte Anatomy 0.000 claims description 3
- 210000000130 stem cell Anatomy 0.000 claims description 3
- 241000237858 Gastropoda Species 0.000 claims description 2
- 210000001789 adipocyte Anatomy 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims description 2
- 230000004888 barrier function Effects 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 210000000988 bone and bone Anatomy 0.000 claims description 2
- 210000004413 cardiac myocyte Anatomy 0.000 claims description 2
- 210000000555 contractile cell Anatomy 0.000 claims description 2
- 210000002919 epithelial cell Anatomy 0.000 claims description 2
- 239000005556 hormone Substances 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims description 2
- 210000005260 human cell Anatomy 0.000 claims description 2
- 210000004962 mammalian cell Anatomy 0.000 claims description 2
- 210000000663 muscle cell Anatomy 0.000 claims description 2
- 230000002107 myocardial effect Effects 0.000 claims description 2
- 210000004498 neuroglial cell Anatomy 0.000 claims description 2
- 229940111202 pepsin Drugs 0.000 claims description 2
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 230000003248 secreting effect Effects 0.000 claims description 2
- 241000657665 Rhopilema esculentum Species 0.000 claims 2
- 150000007513 acids Chemical class 0.000 claims 1
- 238000007876 drug discovery Methods 0.000 claims 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims 1
- 239000000243 solution Substances 0.000 description 39
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical group [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 15
- 239000002953 phosphate buffered saline Substances 0.000 description 15
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 239000004971 Cross linker Substances 0.000 description 8
- 208000027418 Wounds and injury Diseases 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 206010052428 Wound Diseases 0.000 description 7
- 239000000499 gel Substances 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 5
- 230000002776 aggregation Effects 0.000 description 5
- 238000004220 aggregation Methods 0.000 description 5
- 235000019688 fish Nutrition 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000035557 fibrillogenesis Effects 0.000 description 4
- 125000000524 functional group Chemical group 0.000 description 4
- 230000000670 limiting effect Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000004132 cross linking Methods 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 125000001183 hydrocarbyl group Chemical group 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 238000011481 absorbance measurement Methods 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000001879 gelation Methods 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 230000005847 immunogenicity Effects 0.000 description 2
- 230000004962 physiological condition Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000012536 storage buffer Substances 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 1
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 241001441571 Hiodontidae Species 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 241000146383 Rhopilema Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000012598 cell culture matrix Substances 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000000242 nostrum Substances 0.000 description 1
- 230000000399 orthopedic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012306 spectroscopic technique Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000003357 wound healing promoting agent Substances 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
- A61L15/325—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/60—Liquid-swellable gel-forming materials, e.g. super-absorbents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Transplantation (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Dispersion Chemistry (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Cardiology (AREA)
- Rheumatology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
したがって、コラーゲンの代替の供給源が望ましいと、ずっと考えられてきた。
しかし、クラゲコラーゲンに課せられた進化上の制約は、タンパク質が、より低い温度で最適に機能するように進化したことである。このより低い熱安定性のために、生理的温度での使用に適切なクラゲコラーゲンヒドロゲルの生産がこれまで不可能であった。
そのため、細胞培養及び医療用途における使用に適切な、改善されたクラゲコラーゲンヒドロゲルの製造方法が求められていた。
クラゲコラーゲンの抽出
コクカイビゼンクラゲ(Rhizostoma pulmo)からの酸可溶性コラーゲンの抽出
クラゲ(コクカイビゼンクラゲ:Rhizostoma pulmo)材料を、400μmフィルターを通し、保持物を、後で使用するために凍結した。凍結された組織を、通常の攪拌によって、24時間かけて解凍した。完全に解凍したらすぐに、組織を、50%NaOHでpH13にし、さらに24時間、放置した。24時間後、濃酢酸を、濃度0.5Mまで加え、pHを、HClを使用して、3.0に調整した。次に、組織を、3~5日間、放置して、非常に低い固体含有量の粘性溶液を得た。全ての工程を、4℃で実行した。
溶液を、20000RCFで1時間、遠心分離して、全ての残っている固体物質を除去した。遠心分離後、濃度1MまでNaClを添加することによって、コラーゲンを、溶液から沈殿させ、引き続き、さらに20分間、遠心分離して、沈殿させたコラーゲンを得た。次に、ペレットを、0.1M酢酸中で再懸濁し、濃HClによって、pHをpH3に調整した。結果として生じる溶液を、合計10時間、さらに遠心分離し、次に、無色透明のJFC溶液が得られるまで、100kDa濾過膜を組み込んだSartorius Vivaflow200システムによって、0.1M酢酸で6倍量の透析容量で透析濾過した。この場合も先と同様に、全ての工程を、4℃で実行した。
JFCヒドロゲル形成に対する溶液条件の影響
JFCの原線維発生アッセイ-酸可溶性のpH依存
酸可溶性JFCを、3mg/mlに希釈し、10×リン酸緩衝生理食塩水(PBS)に加えて、9:1の比率にした。試料のpHを、1MのNaOHを使用して、pH3、5、6、7、8、及び9に調整した(図1)。次に、試料を、UV-visキュベットに加えて3通り作り、定常に到達するまで、313nmで、通常の吸光度測定を行った。
酸可溶性JFCを、3mg/mlに希釈し、溶液のpHを、1MのNaOHを使用して、pH7.4に調整した。次に、0M、0.69M、1.38M及び2.74MのNaClを含有する10×PBSを加えて、JFC対10×PBS比、9:1にし、必要であれば、pHを、再びpH7.4に調整した。次に、試料を、UV-visキュベットに加えて3通り作り、定常に到達するまで、313nmで、通常の吸光度測定を行った(図2)。
酸可溶性JFCの試料3mg/mlに、10×PBSを加えて、JFC対PBS比、9:1にすることによって、JFCを、ゲル状にした。NaOHを加えて、溶液をpH3、5、6、7、8、及び9に調整した。次に、それぞれのpHの試料を、8時間、4℃又は19℃のいずれかで放置して、ゲルを成長させた。
ヒドロゲルの熱安定性を、pH9及び4℃で、PBS中に形成されるヒドロゲルを使用して研究した。JFC溶液に、2.74MのNaClを補充した。結果として生じるJFCヒドロゲルを、18℃、20℃、22℃、24℃、及び26℃のいずれかで、5分間、水浴槽に浸漬した。変性温度を、ゲルの半分が崩壊する時点として、判定した。
ヒドロゲルの熱安定性を上昇させること
クラゲコラーゲン(JFC)ヒドロゲルの熱安定性を上昇させるクロスリンカーである、ゲニピン、BDDGE、及びムコクロロ酸の能力を、>37℃で長期間安定なJFCヒドロゲルを作る目的で、評価した。
酸で可溶化されたJFC3.5mg/mlの複数のアリコートを、10×リン酸緩衝生理食塩水(PBS)で、9:1(JFC:10×PBS)の比率で希釈した。次に、それぞれの溶液を、濃NaOHを加えることによって、pH3からpH9の間にpH調整した。次に、架橋剤1,4-BDDGE、ゲニピン又はムコクロロ酸を加えて、それぞれ、最終濃度1%から4%まで、又は0.025%から5%まで、又は0.1%から5%にした。次に、溶液を、攪拌して、クロスリンカーを拡散させ、0.5時間から24時間、4℃から25℃でインキュベートして、原線維発生及びゲル化を完成させた。
熱安定性を判定するために、架橋ゲルを、20℃のインキュベーターに入れた。温度を、1時間ごとに1℃上昇させて、熱安定性を記録した(図3)。熱安定性を、ゲルが最初に収縮及び/又は融解し始めた温度と定義した、以下、Tmと呼ぶ。
ゲニピン及びBDDGEの両方の2つの濃度を、使用して、37℃で安定なヒドロゲルを形成させ得る、それぞれのクロスリンカーの可能な最低濃度を判定した。架橋ヒドロゲルを、120時間、pH7で、1%及び4%BDDGE(v/v)ならびに0.025%、0.05%、0.075%及び0.1%ゲニピンで形成し、Tmに対する効果を、(図4)に示す。図で分かる通り、1%BDDGEヒドロゲルは、4%BDDGEヒドロゲルと全く同じTmを有した。両方の濃度は、非架橋ヒドロゲルにおける20℃から、1%及び4%架橋ヒドロゲル両方における35℃まで、Tmの上昇15℃をもたらした。pH8でインキュベートされなかったので、いずれの濃度も、37℃で安定なヒドロゲルを生成しなかった。ゲニピン濃度の全ては、37℃で熱安定性のヒドロゲルを生成し、最低Tmは、0.025%ゲニピン架橋ヒドロゲルにおける43℃であった(図4)。これは、非架橋対照より23℃高い。3つのより高い濃度、0.05%、0.075%及び0.1%GPは全て、Tm45℃を有した。
Claims (22)
- クラゲコラーゲン原線維を含むクラゲコラーゲンヒドロゲルの製造方法であって、前記製造方法は、
a. i.精製されたクラゲコラーゲンの溶液、及び
ii.水性中和緩衝液を混合する工程と、
b.前記混合物を、コラーゲン原線維の形成を可能にするのに十分な長さの時間、インキュベートする工程と、を含み、
架橋剤が、混合工程(a)の間に加えられるか、又は工程(b)から得られた前記コラーゲン原線維に加えられるかのいずれかであるコラーゲンヒドロゲルの製造方法。 - 前記混合工程(a)が、pH4からpH9のpHを有する溶液をもたらす、場合によりpHは7.4である、請求項1に記載のコラーゲンヒドロゲルの製造方法。
- 工程(b)が、4℃から25℃の温度で実施される、請求項1又は2に記載のコラーゲンヒドロゲルの製造方法。
- 前記十分な長さの時間が、12時間以下であり、好ましくは、前記十分な長さの時間が、5から60分である、請求項1から3のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 前記水性中和緩衝液が、リン酸をベースとする、請求項1から4のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 前記架橋剤が、ゲニピン、1,4-BDDGE、又はムコクロロ酸から選択されるものであり、
前記架橋剤が、
a.ゲニピンの場合、最終濃度0.001%から5%、好ましくは0.025%(w/v)で加えられ、
b.1,4-BDDGEの場合、最終濃度0.001%から5%、好ましくは4%(w/v)で加えられ、
c.ムコクロロ酸の場合、最終濃度0.001%から5%、好ましくは、0.25%から4%(w/v)で加えられ、
場合により、前記架橋剤がゲニピン又は1,4-BDDGEである、請求項1から5のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。 - 前記精製されたクラゲコラーゲンの溶液が、酵素抽出によってコラーゲンの供給源から精製されたものであり、場合により前記酵素がペプシンである、請求項1から6のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 前記精製されたクラゲコラーゲンの溶液が、酸抽出によってコラーゲンの供給源から精製された、請求項1から7のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 前記精製されたクラゲコラーゲンの溶液が、コクカイビゼンクラゲ(Rhizostomas pulmo)、ビゼンクラゲ(Rhopilema esculentum)、ロピレマ・ノマディカ(Rhopilema nomadica)、スナクラゲ(Stomolophus meleagris)、ミズクラゲ(Aurelia sp.)、エチゼンクラゲ(Nemopilema nomurai)、又はそれらの組合せから精製されたものであり、場合により前記精製されたクラゲコラーゲンの溶液が、コクカイビゼンクラゲ(Rhizostomas pulmo)から精製された、請求項1から8のいずれか一項に記載のコラーゲンヒドロゲルの製造方法。
- 請求項1から9のいずれか一項に記載のコラーゲンヒドロゲルの製造方法から得ることができるものであり、前記コラーゲンヒドロゲルは25℃から50℃の温度で安定であるクラゲコラーゲンヒドロゲル。
- 25℃から50℃の温度で安定な単離されたものであり、前記コラーゲンヒドロゲルは中和緩衝液及び架橋剤を含み、前記コラーゲンヒドロゲルはクラゲコラーゲン原線維を含むクラゲコラーゲンヒドロゲル。
- 3D細胞培養足場の生産における、請求項10又は請求項11に記載の前記クラゲコラーゲンヒドロゲルの使用。
- 医療用デバイスの生産において、請求項10又は請求項11に記載の前記クラゲコラーゲンヒドロゲルの使用であって、場合により前記医療用デバイスが、創傷被覆材である、使用。
- 薬物送達のための担体としての、請求項10又は請求項11に記載の前記クラゲコラーゲンヒドロゲルの使用。
- 請求項10又は請求項11に記載の前記クラゲコラーゲン足場を含む医療用デバイスであって、場合により前記医療用デバイスが創傷被覆材である、医療用デバイス。
- 請求項10又は請求項11に記載のクラゲコラーゲンヒドロゲルを含む細胞培養足場。
- 請求項10又は請求項11に記載の前記クラゲコラーゲンヒドロゲルを含む薬物送達のための担体。
- 細胞を培養するための、請求項16に記載の細胞培養足場の使用であって、場合により前記細胞がヒトの細胞である細胞培養足場の使用。
- 前記細胞が、肝細胞、筋細胞、心筋細胞、ケラチノサイト、脂肪細胞、ニューロン、腎細胞、上皮細胞、グリア細胞、ホルモン分泌細胞、バリア機能細胞、細胞外マトリックス細胞、収縮性細胞、水晶体細胞、幹細胞、間葉系幹細胞、血液由来の幹細胞、人工多能性幹細胞、又はそれらの組合せからなる群から選択される、初代哺乳類細胞であって、
場合により前記細胞が肝細胞又は心筋細胞である、請求項18に記載の使用。 - (i)前記細胞が、培養されて、創薬における使用のための3D細胞組織構造を形成する、
(ii)前記細胞が、培養されて、角膜シールドを形成する、
(iii)前記細胞が、培養されて、軟膏を生成する、
(iv)前記細胞が、培養されて、骨を形成する、
(v)前記細胞が、培養されて、ニューロンを形成する、
(vi)前記細胞が、共培養できる、
請求項18に記載の使用。 - 請求項1に記載のコラーゲンヒドロゲルの製造方法に使用するためのキットであって、
前記キットは、精製されたクラゲコラーゲンの溶液、中和緩衝液及び架橋剤を含み、場合により前記精製されたクラゲコラーゲンの溶液が、0.1mg/mlから30mg/mlの範囲内の濃度であるキット。 - 請求項21に記載のコラーゲンヒドロゲルの製造方法に使用するためのキットであって、
前記中和緩衝液が、遊離アミンを含まず、好ましくは、前記中和緩衝液が、リン酸をベースとするものであり、場合により前記架橋剤が、ゲニピン、1,4-BDDGE、又はムコクロロ酸から選択されるキット。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1615205.0 | 2016-09-07 | ||
GBGB1615205.0A GB201615205D0 (en) | 2016-09-07 | 2016-09-07 | Method |
PCT/GB2017/052607 WO2018046920A1 (en) | 2016-09-07 | 2017-09-07 | Method for producing collagen hydrogels |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2019529538A JP2019529538A (ja) | 2019-10-17 |
JP2019529538A5 JP2019529538A5 (ja) | 2020-09-03 |
JP7062667B2 true JP7062667B2 (ja) | 2022-05-06 |
Family
ID=57139999
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2019533705A Active JP7062667B2 (ja) | 2016-09-07 | 2017-09-07 | コラーゲンヒドロゲルの製造方法 |
Country Status (9)
Country | Link |
---|---|
US (1) | US11369714B2 (ja) |
EP (1) | EP3509649B1 (ja) |
JP (1) | JP7062667B2 (ja) |
KR (2) | KR102606472B1 (ja) |
DK (1) | DK3509649T3 (ja) |
ES (1) | ES2870612T3 (ja) |
GB (1) | GB201615205D0 (ja) |
PT (1) | PT3509649T (ja) |
WO (1) | WO2018046920A1 (ja) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102098134B1 (ko) * | 2019-02-13 | 2020-04-08 | 성균관대학교산학협력단 | 나노 섬유질 콜라겐 3차원 구조체 제조방법 |
GB201908709D0 (en) * | 2019-06-18 | 2019-07-31 | Jellagen Pty Ltd | Methacrylated collagen |
GB202005141D0 (en) * | 2020-04-07 | 2020-05-20 | Jellagen Pty Ltd | Jellyfish collagen use |
CN112501108B (zh) * | 2020-11-09 | 2023-04-07 | 中国人民解放军海军军医大学 | 沙蜇触手大、小刺丝囊的分离与毒液提取方法 |
WO2022237900A1 (en) * | 2021-05-13 | 2022-11-17 | Shanghai Qisheng Biological Preparation Co., Ltd. | Derivatized or rapidly polymerizing collagen compositions for tissue augmentation containing non-resorbable or slowly resorbable polymers |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005079879A1 (ja) | 2004-02-25 | 2005-09-01 | Ihara & Company Ltd. | コラーゲンゲルおよびその製造方法 |
JP2011509734A (ja) | 2008-01-16 | 2011-03-31 | コル−メド リミテッド | クラゲから製造されるコロイドコラーゲンの火傷包帯 |
WO2014106830A1 (en) | 2013-01-07 | 2014-07-10 | Ramot At Tel-Aviv University Ltd. | Jellyfish-derived polymer |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2801313A1 (fr) | 1999-05-19 | 2001-05-25 | Coletica | Produit collagenique contenant du collagene d'origine marine a faible odeur et de preference a proprietes mecaniques ameliorees, ainsi que son utilisation sous forme de compositions ou de produits cosmetiques ou pharmaceutiques |
KR20060091350A (ko) * | 2005-02-14 | 2006-08-21 | 한양대학교 산학협력단 | 해양생물로부터 추출된 콜라겐을 이용하여 제조된 조직공학용 고분자 지지체 및 그 콜라겐의 추출방법 |
US20100111919A1 (en) | 2008-10-31 | 2010-05-06 | Tyco Healthcare Group Lp | Delayed gelation compositions and methods of use |
EP2644692B1 (en) * | 2010-11-26 | 2017-08-30 | Tokyo Institute of Technology | High-strength collagen fiber membrane and method for producing same |
EP3034103A1 (en) * | 2014-12-15 | 2016-06-22 | Geistlich Pharma AG | Collagen Sponge |
GB201506236D0 (en) | 2015-04-13 | 2015-05-27 | Jellagen Pty Ltd | Modified collagen, methods of manufacture thereof |
-
2016
- 2016-09-07 GB GBGB1615205.0A patent/GB201615205D0/en not_active Ceased
-
2017
- 2017-09-07 WO PCT/GB2017/052607 patent/WO2018046920A1/en unknown
- 2017-09-07 US US16/330,862 patent/US11369714B2/en active Active
- 2017-09-07 EP EP17767893.5A patent/EP3509649B1/en active Active
- 2017-09-07 ES ES17767893T patent/ES2870612T3/es active Active
- 2017-09-07 JP JP2019533705A patent/JP7062667B2/ja active Active
- 2017-09-07 PT PT177678935T patent/PT3509649T/pt unknown
- 2017-09-07 KR KR1020197009932A patent/KR102606472B1/ko active IP Right Grant
- 2017-09-07 DK DK17767893.5T patent/DK3509649T3/da active
- 2017-09-07 KR KR1020237040119A patent/KR20230163000A/ko not_active Application Discontinuation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005079879A1 (ja) | 2004-02-25 | 2005-09-01 | Ihara & Company Ltd. | コラーゲンゲルおよびその製造方法 |
JP2011509734A (ja) | 2008-01-16 | 2011-03-31 | コル−メド リミテッド | クラゲから製造されるコロイドコラーゲンの火傷包帯 |
WO2014106830A1 (en) | 2013-01-07 | 2014-07-10 | Ramot At Tel-Aviv University Ltd. | Jellyfish-derived polymer |
Non-Patent Citations (5)
Title |
---|
HOYER B. et al,Acta Biomaterialia,2013年10月30日,10,p.883-892 |
KOH L. et al.,J. Funct. Biomater.,2013年,4,p.162-177 |
YUNOKI S. et al.,International Journal of Biomaterials,2013年,Volume 2013,Article ID 620765 |
奥山 健二 他,高分子論文集,2010年,Vol.67 No.4,p.229-247 |
鈴木 啓二 他,日本写真学会誌,2004年,67巻1号,p.67-73 |
Also Published As
Publication number | Publication date |
---|---|
PT3509649T (pt) | 2021-06-02 |
WO2018046920A1 (en) | 2018-03-15 |
US20190216972A1 (en) | 2019-07-18 |
US11369714B2 (en) | 2022-06-28 |
ES2870612T3 (es) | 2021-10-27 |
EP3509649A1 (en) | 2019-07-17 |
KR102606472B1 (ko) | 2023-11-28 |
KR20190055124A (ko) | 2019-05-22 |
JP2019529538A (ja) | 2019-10-17 |
DK3509649T3 (en) | 2021-04-06 |
KR20230163000A (ko) | 2023-11-29 |
EP3509649B1 (en) | 2021-02-24 |
GB201615205D0 (en) | 2016-10-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP7062667B2 (ja) | コラーゲンヒドロゲルの製造方法 | |
Wang et al. | Exploring natural silk protein sericin for regenerative medicine: an injectable, photoluminescent, cell-adhesive 3D hydrogel | |
JP6138370B2 (ja) | セリシンハイドロゲルの調製方法と使用 | |
JP4064435B2 (ja) | コラーゲンゲルおよびその製造方法 | |
CN110041536B (zh) | 功能性丝胶蛋白水凝胶及其制备方法和应用 | |
US9315562B2 (en) | High-strength collagen fiber membrane and a manufacturing method thereof | |
JP2006257013A (ja) | 魚鱗由来コラーゲンゲルとその作成方法 | |
Lai | Influence of solvent composition on the performance of carbodiimide cross-linked gelatin carriers for retinal sheet delivery | |
JP5875761B2 (ja) | コラーゲン線維ゲルおよびその用途 | |
JP3412014B2 (ja) | ハイドロゲルの製造方法および細胞培養支持体 | |
US8877500B2 (en) | Thermally induced gelation of collagen hydrogel and method of thermally inducing gelling a collagen hydrogel | |
RU2646804C1 (ru) | Офтальмологическое средство для регенерации роговицы глаза | |
WO2020075841A1 (ja) | バラマンディ鱗由来コラーゲンを含む細胞培養基材 | |
US20220305168A1 (en) | Method for producing collagen hydrogels | |
WO2003094985A1 (fr) | Matrice extracellulaire artificielle et procede de fabrication associe | |
CN105087482B (zh) | 一种细胞培养基质及其应用与使用方法 | |
JP6432967B2 (ja) | 溶解性コラーゲン線維多孔体 | |
JPWO2002102845A1 (ja) | 絹フィブロイン由来機能性ポリペプチドの製造法及びその利用 | |
KR102262206B1 (ko) | 망막색소상피세포를 포함하는 하이드로겔 조성물 및 이의 용도 | |
JP3542818B2 (ja) | Iv型コラーゲンゲルの製造方法 | |
CN115286709A (zh) | 一种可自组装的三螺旋重组胶原蛋白及其交联形成的凝胶 | |
Zeugolis et al. | Reformed Collagen Fibres |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200720 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20200720 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20210514 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20210526 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20210826 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210909 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20211222 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20220317 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20220330 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20220420 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 7062667 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |