JP6772150B2 - 水中試験における線虫およびその他の生物に対する有効成分の活性を測定する装置および方法 - Google Patents
水中試験における線虫およびその他の生物に対する有効成分の活性を測定する装置および方法 Download PDFInfo
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- JP6772150B2 JP6772150B2 JP2017538697A JP2017538697A JP6772150B2 JP 6772150 B2 JP6772150 B2 JP 6772150B2 JP 2017538697 A JP2017538697 A JP 2017538697A JP 2017538697 A JP2017538697 A JP 2017538697A JP 6772150 B2 JP6772150 B2 JP 6772150B2
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- 238000004113 cell culture Methods 0.000 claims description 50
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- 241000244203 Caenorhabditis elegans Species 0.000 description 6
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5085—Supracellular entities, e.g. tissue, organisms of invertebrates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
- G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T5/00—Image enhancement or restoration
- G06T5/20—Image enhancement or restoration using local operators
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/20—Analysis of motion
- G06T7/254—Analysis of motion involving subtraction of images
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N2021/1765—Method using an image detector and processing of image signal
- G01N2021/177—Detector of the video camera type
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/063—Illuminating optical parts
- G01N2201/0638—Refractive parts
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
- G01N2333/43526—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from worms
- G01N2333/4353—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from worms from nematodes
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
- G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
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- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
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- Computer Vision & Pattern Recognition (AREA)
- Toxicology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
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- Multimedia (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medical Informatics (AREA)
- Radiology & Medical Imaging (AREA)
- Quality & Reliability (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
10 ハウジング
11 カメラ
12 対物レンズ
13 ホルダー
14 照明機構
15 第一光源
16 第二光源
17 ロッドレンズ(17a、17b出射光線)
18 ロッドレンズ(18a、18b出射光線)
30 細胞培養皿
31 ウェル
32 上部
33 底部
34 第一側壁
35 第二側壁
36 溶液
37 ウェル・カバー
100 出発ブロック
101 ブロック
102 ブロック
103 ブロック
104 ブロック
105 ブロック
106 ブロック
107 ループ
108 質問
109 ブロック
110 ループ
111 質問
112 ブロック
113 ブロック
114 ブロック
Claims (7)
- 水中試験における線虫またはその他の生物に対する有効成分の活性を測定する方法であって、
a)細胞培養皿(30)の少なくとも1つのウェル(31)を前記生物および有効成分で充填する工程;
b)細胞培養皿(30)を、以下の(i)〜(iii):
(i)中で生物を有効成分で充填できる複数のウェル(31)を備える細胞培養皿(30)のためのホルダー(13)であって、該細胞培養皿(30)が、底部(33)および上部(32)とともに、底部(33)と上部(32)との間に伸びる側壁を備えるものである、ホルダー(13);
(ii)好ましくは細胞培養皿(30)の底部(33)、の画像を記録するために用いられるカメラ(11)
(iii)細胞培養皿(30)を照射する第一光源(15)を少なくとも備えている照明機構(14);
を含んでなる装置(1)であって、
第一光源(15)と取り付けられた状態の細胞培養皿(30)の第一側壁(34)との間に、第一光源(15)の光を、第一側壁(34)を通過して、細胞培養皿(30)の底部(33)の方向へ方向づける第一光学装置が配置されている装置(1)中に、配置する工程;
c)経時的に次から次へと続く、細胞培養皿(30)の複数の画像を作成する工程;
d)細胞培養皿(30)の照射に依存して作成された画像を二値化する工程;
e)1つの基本画像および複数の追跡画像を有する第一組の画像について、ウェルに基づき、第一測定曲線を決定する工程であって、各追跡画像が差分法にて基本画像と比較され、差分ピクセル数が決定される、工程;
f)第二組の画像について、ウェルに基づき、少なくとも第二測定曲線を決定する工程であって、第二組に対しては、第二組の基本画像として第一組の追跡画像が使用され、第二組の追跡画像として少なくとも1つの第一組のさらなる追跡画像が使用される、工程;
g)第一測定曲線および少なくとも第二測定曲線に基づいて平均曲線を決定する工程
を含んでなる、方法。 - 前記第二組の基本画像に使用するものが、第一組の基本画像に直ちに続く第一組の第一追跡画像であり、第二組の追跡画像として第二組に使用されるものが、第一組の第一追跡画像を除く全ての第一組の追跡画像であり、かつ、第二組が、1個の追加の追跡画像によって完成されることを特徴とする、請求項1に記載の方法。
- 1つの組の基本画像と最終追跡画像との間の記録時間(TA)が、該記録時間(TA)内に、未処置生物が中に入れられたウェルについての差分ピクセル数が漸近極限AWに達するように確立されることを特徴とする、請求項1または2に記載の方法。
- 第一組の基本画像と最終組の最終追跡画像との間に総時間が確立されることを特徴とし、その総時間が、記録時間(TA)のおよそ2倍に相当することを特徴とする、請求項3に記載の方法。
- 2個の連続する画像間の時間間隔が一定であり、かつ1〜5秒であることを特徴とする、請求項1〜4のいずれか一項に記載の方法。
- 1つの組の画像が、8〜12個の画像を含むことを特徴とする、請求項1〜5のいずれか一項に記載の方法。
- 前記平均曲線が、8〜12の測定曲線に基づいて決定されることを特徴とする、請求項2〜6のいずれか一項に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP15152272 | 2015-01-23 | ||
EP15152272.9 | 2015-01-23 | ||
PCT/EP2016/050140 WO2016116291A1 (de) | 2015-01-23 | 2016-01-06 | Verfahren und vorrichtung zur ermittlung der wirkung von wirkstoffen an nematoden und anderen organismen in wässrigen tests |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2018503097A JP2018503097A (ja) | 2018-02-01 |
JP6772150B2 true JP6772150B2 (ja) | 2020-10-21 |
Family
ID=52396529
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2017538697A Active JP6772150B2 (ja) | 2015-01-23 | 2016-01-06 | 水中試験における線虫およびその他の生物に対する有効成分の活性を測定する装置および方法 |
Country Status (10)
Country | Link |
---|---|
US (2) | US10514375B2 (ja) |
EP (1) | EP3247990B1 (ja) |
JP (1) | JP6772150B2 (ja) |
KR (1) | KR102477339B1 (ja) |
CN (1) | CN107278268B (ja) |
AU (2) | AU2016208790A1 (ja) |
BR (1) | BR112017015691B1 (ja) |
ES (1) | ES2958398T3 (ja) |
HK (1) | HK1245883A1 (ja) |
WO (1) | WO2016116291A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3330697A1 (de) | 2016-11-30 | 2018-06-06 | Bayer Aktiengesellschaft | Vorrichtung zur ermittlung der wirkung von wirkstoffen an nematoden und anderen organismen in wässrigen tests |
GB2578144A (en) * | 2018-10-18 | 2020-04-22 | The Univ Of Durham | Method and apparatus for tracking nematode worms |
US11492585B2 (en) * | 2019-05-31 | 2022-11-08 | Canon Medical Systems Corporation | Cell identification system and cell identification method |
US20230105170A1 (en) * | 2020-02-28 | 2023-04-06 | Thrive Bioscience, Inc. | Pre-scan focus and scan focus methods |
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JPS6170407A (ja) * | 1984-08-08 | 1986-04-11 | Canon Inc | 距離測定装置 |
WO1994005770A1 (es) | 1992-08-27 | 1994-03-17 | Iul, S.A. | Camara de contraste para realzar colonias bacterianas respecto a la base de cultivo de las mismas |
TW391139B (en) * | 1996-12-26 | 2000-05-21 | Plus Kk | Image display device |
JPH10197449A (ja) * | 1997-01-07 | 1998-07-31 | Hamamatsu Photonics Kk | 光測定装置 |
JPH11183358A (ja) * | 1997-12-25 | 1999-07-09 | Kowa Co | 蛍光粒子撮像用容器 |
JP2000074837A (ja) * | 1998-08-31 | 2000-03-14 | Fuji Photo Film Co Ltd | 撮影装置 |
GB9908676D0 (en) * | 1999-04-15 | 1999-06-09 | Devgen Nv | Method for screening compounds |
US20040101954A1 (en) * | 2002-11-27 | 2004-05-27 | Graessle Josef A. | Back side plate illumination for biological growth plate scanner |
US7265829B2 (en) | 2002-12-17 | 2007-09-04 | Molecular Devices Corporation | Reflective optic system for imaging microplate readers |
FI116946B (fi) * | 2004-07-09 | 2006-04-13 | Chip Man Technologies Oy | Laitteisto solujen kuvaamiseksi |
CN1800340A (zh) * | 2004-12-31 | 2006-07-12 | 王衍淞 | 获取培养皿内菌落图像时使用的照明方法 |
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JP4921458B2 (ja) | 2005-04-04 | 2012-04-25 | ブルーシフト・バイオテクノロジーズ・インコーポレーテッド | Fret発光における偏光異方性を使用したスクリーニングの方法 |
JP4444984B2 (ja) * | 2007-04-18 | 2010-03-31 | アドバンスド・マスク・インスペクション・テクノロジー株式会社 | レチクル欠陥検査装置およびこれを用いた検査方法 |
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JP2011059095A (ja) * | 2009-08-12 | 2011-03-24 | Sony Corp | 光検出装置 |
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EP2564111B1 (en) * | 2010-04-28 | 2022-01-05 | KLA-Tencor Corporation | Ring light illuminator and beam shaper for ring light illuminator |
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WO2012014141A1 (en) * | 2010-07-30 | 2012-02-02 | Kla-Tencor Corporation | Ring light illuminator, beam shaper and method for illumination |
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-
2016
- 2016-01-06 KR KR1020177021842A patent/KR102477339B1/ko not_active Application Discontinuation
- 2016-01-06 WO PCT/EP2016/050140 patent/WO2016116291A1/de active Application Filing
- 2016-01-06 BR BR112017015691-1A patent/BR112017015691B1/pt active IP Right Grant
- 2016-01-06 EP EP16700534.7A patent/EP3247990B1/de active Active
- 2016-01-06 AU AU2016208790A patent/AU2016208790A1/en not_active Abandoned
- 2016-01-06 ES ES16700534T patent/ES2958398T3/es active Active
- 2016-01-06 US US15/544,409 patent/US10514375B2/en active Active
- 2016-01-06 JP JP2017538697A patent/JP6772150B2/ja active Active
- 2016-01-06 CN CN201680006689.6A patent/CN107278268B/zh active Active
-
2018
- 2018-04-18 HK HK18105001.6A patent/HK1245883A1/zh unknown
-
2019
- 2019-11-13 US US16/682,666 patent/US10948481B2/en active Active
-
2021
- 2021-06-22 AU AU2021204224A patent/AU2021204224B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
HK1245883A1 (zh) | 2018-08-31 |
ES2958398T3 (es) | 2024-02-08 |
US20200080992A1 (en) | 2020-03-12 |
US20180011084A1 (en) | 2018-01-11 |
KR102477339B1 (ko) | 2022-12-14 |
US10514375B2 (en) | 2019-12-24 |
BR112017015691A2 (pt) | 2018-04-10 |
US10948481B2 (en) | 2021-03-16 |
EP3247990B1 (de) | 2023-07-05 |
AU2021204224B2 (en) | 2023-07-13 |
AU2021204224A1 (en) | 2021-07-15 |
AU2016208790A1 (en) | 2017-08-10 |
CN107278268A (zh) | 2017-10-20 |
JP2018503097A (ja) | 2018-02-01 |
KR20170103872A (ko) | 2017-09-13 |
BR112017015691B1 (pt) | 2021-06-08 |
EP3247990A1 (de) | 2017-11-29 |
CN107278268B (zh) | 2022-02-08 |
WO2016116291A1 (de) | 2016-07-28 |
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