JP6671725B2 - 選択的平面照明顕微鏡法機器 - Google Patents
選択的平面照明顕微鏡法機器 Download PDFInfo
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- JP6671725B2 JP6671725B2 JP2017533907A JP2017533907A JP6671725B2 JP 6671725 B2 JP6671725 B2 JP 6671725B2 JP 2017533907 A JP2017533907 A JP 2017533907A JP 2017533907 A JP2017533907 A JP 2017533907A JP 6671725 B2 JP6671725 B2 JP 6671725B2
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- 238000005286 illumination Methods 0.000 title claims description 78
- 238000000386 microscopy Methods 0.000 title claims description 16
- 230000003287 optical effect Effects 0.000 claims description 64
- 238000000034 method Methods 0.000 claims description 10
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- 230000001902 propagating effect Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims 1
- 238000003384 imaging method Methods 0.000 description 11
- 230000008901 benefit Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000000799 fluorescence microscopy Methods 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 241000244203 Caenorhabditis elegans Species 0.000 description 1
- 206010034972 Photosensitivity reaction Diseases 0.000 description 1
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Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/086—Condensers for transillumination only
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0088—Inverse microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Description
100 光学配置
110 SPIM照明対物レンズ、反転照明対物レンズ
111 照明対物レンズ光軸
120 試料支持部
122 試料保持領域
124 浸漬油
130 画像収集対物レンズ
131 画像収集対物レンズ光軸
132 コヒーレントレーザ光ビーム、照明ビーム、照明ビーム経路
140 画像収集対物レンズ
141 光軸
142 コヒーレントレーザ光ビーム、照明ビーム、照明ビーム経路
Claims (10)
- 試料支持部(120)の下方に取り付けられて使用時に前記試料支持部(120)で光の線または平面を提供する反転照明対物レンズ(110)と、前記支持部(120)の上方に取り付けられた第1の画像収集対物レンズ(130)及び第2の画像収集対物レンズ(140)と、を含み、前記反転照明対物レンズ(110)は、照明対物レンズ光軸(111)を有し、前記第1の画像収集対物レンズ(130)及び前記第2の画像収集対物レンズ(140)のそれぞれは、画像収集対物レンズ光軸(131)及び画像像収集対物レンズ光軸(141)を有する、選択的平面照明を提供する光学配置(100)であって、切り替え可能な照明光(132)及び照明光(142)は、前記照明対物レンズ光軸(111)に対して互に平行であると共に前記照明対物レンズ光軸(111)に対してそれぞれ横方向にオフセットされて前記照明対物レンズ(110)に向かって伝搬するように構成され、それにより前記照明対物レンズ(110)から出る前記照明光(132)及び前記照明光(142)は、前記照明対物レンズ光軸(111)に対して斜めの角度で前記試料支持部(120)に向かって伝搬し、前記画像収集対物レンズ光軸(131)及び前記画像像収集対物レンズ光軸(141)はそれぞれ、前記照明対物レンズ光軸(111)に対して鈍角である角度αを成しており、前記試料支持部(120)で伝搬する光にほぼ垂直である、光学配置(100)。
- 前記角度αが、100〜170度である、請求項1に記載の光学配置(100)。
- 前記照明光(132)及び前記照明光(142)が、前記第1または第2の画像収集対物レンズ(130,140)に交互に画像光を提供するように、それぞれの第1および第2の経路に沿って前記照明対物レンズ光軸(111)に対して互いに反対側に交互に伝搬するように構成される、請求項1または2に記載の光学配置(100)。
- 前記照明光(132)及び前記照明光(142)が、コヒーレント光であり、前記光学配置(100)がさらに、光線に集光した、またはコリメートされた光の平面またはシートの形態の前記照明光(132)及び前記照明光(142)を提供する光学マニピュレータを含む、請求項1、2または3に記載の光学配置(100)。
- 試料を照明してSPIMを提供する方法であって、
a)請求項3または4に記載の光学配置(100)を提供するステップと、
b)コヒーレントな照明光(132)及び照明光(142)を照明の方向に伝搬させるステップと、
c)前記第1および第2の画像収集対物レンズ(130,140)によって収集された1つまたは複数の画像を捕捉するステップと、
を含む、方法。 - 請求項1〜4のいずれか1項に記載の光学配置(100)を含む、SPIM機器(10)。
- SIM、TIRFおよびPK顕微鏡法技術の任意の1つまたは複数をさらに実行するように適合可能な、請求項6に記載のSPIM機器(10)。
- 前記照明対物レンズ(110)と前記試料支持部(120)との間に空間があり、前記空間が、液体によって架橋される、請求項6または7に記載のSPIM機器(10)。
- 前記試料支持部(120)が、好ましくは連続動作で前記照明対物レンズ光軸(111)に垂直に移動可能である、請求項6、7または8に記載のSPIM機器(10)。
- 照明対物レンズ光軸(111)を有する照明対物レンズ(110)と、前記照明対物レンズ光軸(111)に垂直な方向に前記光軸(111)に対して、好ましくは連続動作で移動可能な試料支持部(120)とを含む、SPIM機器(10)。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462095959P | 2014-12-23 | 2014-12-23 | |
US62/095,959 | 2014-12-23 | ||
US201562109245P | 2015-01-29 | 2015-01-29 | |
US62/109,245 | 2015-01-29 | ||
PCT/US2015/064512 WO2016105934A2 (en) | 2014-12-23 | 2015-12-08 | Selective plane illumination microscopy instruments |
Publications (2)
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JP2018506733A JP2018506733A (ja) | 2018-03-08 |
JP6671725B2 true JP6671725B2 (ja) | 2020-03-25 |
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JP2017533907A Active JP6671725B2 (ja) | 2014-12-23 | 2015-12-08 | 選択的平面照明顕微鏡法機器 |
Country Status (5)
Country | Link |
---|---|
US (1) | US10481375B2 (ja) |
EP (1) | EP3237949A4 (ja) |
JP (1) | JP6671725B2 (ja) |
CN (1) | CN107003508B (ja) |
WO (1) | WO2016105934A2 (ja) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
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EP3341710B1 (en) * | 2015-08-26 | 2022-02-16 | The United States of America, as represented by The Secretary, Department of Health & Human Services | Systems and methods for multi-view nonlinear optical imaging for improved signal-to-noise ratio and resolution in point scanning multi-photon microscopy |
DE102016120683A1 (de) | 2016-10-28 | 2018-05-03 | Carl Zeiss Microscopy Gmbh | Lichtblattmikroskop |
DE102017204325A1 (de) * | 2017-03-15 | 2018-09-20 | Carl Zeiss Microscopy Gmbh | Anordnung, Mikroskop und Verfahren zur TIRF-Mikroskopie |
DE102017214189A1 (de) * | 2017-08-15 | 2019-02-21 | Carl Zeiss Microscopy Gmbh | Verfahren zum Betrieb einer Mikroskopieranordnung und Mikroskopieranordnung mit einem ersten Mikroskop und mindestens einem weiteren Mikroskop |
JP6876576B2 (ja) * | 2017-08-17 | 2021-05-26 | 日本電子株式会社 | 三次元像構築方法 |
GB201718587D0 (en) * | 2017-11-10 | 2017-12-27 | Luxendo Gmbh | Microscope stage |
EP3495865A1 (en) * | 2017-12-07 | 2019-06-12 | European Molecular Biology Laboratory | A sample holder for imaging a plurality of samples |
US11885946B2 (en) * | 2018-01-26 | 2024-01-30 | University Of Washington | Apparatuses and methods for multi-direction digital scanned light sheet microscopy |
CN109765684B (zh) * | 2019-03-03 | 2022-03-18 | 北京工业大学 | 多光路多方位实时显微成像*** |
EP4058834A4 (en) * | 2019-11-13 | 2023-12-20 | University of Washington | OPEN TOP LENS MICROSCOPE WITH NON-ORTHOGONAL ARRANGEMENT OF ILLUMINATION AND COLLECTING LENSES |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AT353497B (de) | 1972-05-23 | 1979-11-12 | Leitz Ernst Gmbh | Vorrichtung an mikroskopen, zum automatischen fokussieren des geraetes auf unterschiedliche objekt-ebenen |
US5305139A (en) * | 1991-04-19 | 1994-04-19 | Unimat (Usa) Corporation, Formerly United Holdings, Inc. | Illumination system and method for a high definition 3-D light microscope |
US5570228A (en) * | 1991-04-19 | 1996-10-29 | Edge Scientific Instrument Company Llc | Fiber optic illumination system and method for a high definition light microscope |
US5774224A (en) * | 1997-01-24 | 1998-06-30 | International Business Machines Corporation | Linear-scanning, oblique-viewing optical apparatus |
WO2007124437A2 (en) * | 2006-04-20 | 2007-11-01 | Washington University In St. Louis | Objective-coupled selective plane illumination microscopy |
DE202008018428U1 (de) | 2007-10-22 | 2013-10-09 | Tecan Trading Ag | Objektträger-Transportvorrichtung für ein Laser Scanner-Gerät |
WO2010014244A2 (en) * | 2008-07-30 | 2010-02-04 | The Regents Of The University Of California, San Francisco | Multidirectional selective plane illumination microscopy |
DE102010060121C5 (de) * | 2010-10-22 | 2023-09-28 | Leica Microsystems Cms Gmbh | SPIM-Mikroskop mit sequenziellem Lightsheet |
DE102011000835C5 (de) * | 2011-02-21 | 2019-08-22 | Leica Microsystems Cms Gmbh | Abtastmikroskop und Verfahren zur lichtmikroskopischen Abbildung eines Objektes |
US9316824B2 (en) * | 2011-03-04 | 2016-04-19 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Optomechanical module for converting a microscope to provide selective plane illumination microscopy |
DE102011051042B4 (de) * | 2011-06-14 | 2016-04-28 | Leica Microsystems Cms Gmbh | Abtastmikroskop und Verfahren zur lichtmikroskopischen Abbildung eines Objektes |
DE102011054914A1 (de) | 2011-10-28 | 2013-05-02 | Leica Microsystems Cms Gmbh | Verfahren und Anordnung zur Beleuchtung einer Probe |
JP2013130686A (ja) * | 2011-12-21 | 2013-07-04 | Canon Inc | 撮像装置 |
DE102012110077A1 (de) * | 2012-10-23 | 2014-06-26 | Karlsruher Institut für Technologie | Mikroskop mit mindestens einem Beleuchtungsstrahl in Form einer Lichtscheibe |
US9874736B2 (en) * | 2013-04-29 | 2018-01-23 | The Regents Of The University Of California | Apparatus and method for an inclined single plane imaging microscope box (iSPIM box) |
EP3198326A4 (en) * | 2014-09-24 | 2018-05-23 | The United States of America, as represented by The Secretary, Department of Health and Human Services | Resolution enhancement for light sheet microscopy systems and methods |
-
2015
- 2015-12-08 JP JP2017533907A patent/JP6671725B2/ja active Active
- 2015-12-08 EP EP15874101.7A patent/EP3237949A4/en active Pending
- 2015-12-08 US US15/536,490 patent/US10481375B2/en active Active
- 2015-12-08 CN CN201580070405.5A patent/CN107003508B/zh active Active
- 2015-12-08 WO PCT/US2015/064512 patent/WO2016105934A2/en active Application Filing
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US20170371140A1 (en) | 2017-12-28 |
JP2018506733A (ja) | 2018-03-08 |
CN107003508A (zh) | 2017-08-01 |
WO2016105934A2 (en) | 2016-06-30 |
CN107003508B (zh) | 2020-07-10 |
EP3237949A2 (en) | 2017-11-01 |
WO2016105934A3 (en) | 2016-08-18 |
EP3237949A4 (en) | 2018-08-01 |
US10481375B2 (en) | 2019-11-19 |
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