JP6298450B2 - 固体ナノポアの大きさを制御するための方法 - Google Patents
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- B81C1/00—Manufacture or treatment of devices or systems in or on a substrate
- B81C1/00015—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems
- B81C1/00023—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems without movable or flexible elements
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- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
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Description
本出願は、2012年5月7日にファイルされた米国仮特許出願第61/643,651号および2013年3月14日にファイルされた米国仮特許出願第61/781,081号の優先権の利益を主張する。上記出願各々の開示内容全体を、本明細書に援用する。
Claims (13)
- 膜に形成されたナノポアの大きさを正確に拡大するための方法であって、
a)0.1ボルト毎ナノメートルを超える電界をナノポアに誘導する電位の値を選択する工程と、
b)イオン溶液中にある前記ナノポアに、あらかじめ定められた時間、前記選択された値の電位を印加することによって前記膜の材料を除去し、前記ナノポアを拡大する工程と、
c)前記あらかじめ定められた時間経過後、前記ナノポアに印加される前記電位を前記選択された値未満の値まで下げる工程と、
d)前記下げられた値で電位を印加しながら、前記ナノポアを流れる電流を測定する工程と、
e)前記測定された電流に部分的に基づいて、前記ナノポアの大きさを決定する工程と、を含む、方法。 - 前記測定された電流が閾値以上である場合に、前記ナノポアに印加される前記電位を消失させる工程をさらに含む、請求項1に記載の方法。
- f)前記測定された電流が閾値未満である場合に、前記ナノポアに印加される前記電位を前記選択された値まで高める工程と、
g)前記測定された電流が前記閾値以上になるまで、工程(b)〜(f)を繰り返す工程と、をさらに含む、請求項2に記載の方法。 - 前記電界が約0.3ボルト毎ナノメートルになるように前記電位の値を選択する工程をさらに含む、請求項1に記載の方法。
- 前記電界が0.10ボルト毎ナノメートルから0.4ボルト毎ナノメートルの範囲に入るように前記電位の値を選択する工程をさらに含む、請求項1に記載の方法。
- オーム性イオン電流を安定させることができるだけの時間、前記下げられた値で前記電位を印加する工程をさらに含む、請求項1に記載の方法。
- 前記測定された電流が前記閾値未満である場合に、前記選択された値の電位を、極性を反転させて前記ナノポアに再印加する工程をさらに含む、請求項2に記載の方法。
- 前記ナノポアの拡径速度を高めるために、前記電位の大きさを増す工程をさらに含む、請求項1に記載の方法。
- 前記ナノポアの拡径速度を高めるために、前記イオン溶液のイオン強度を高める工程をさらに含む、請求項1に記載の方法。
- 膜に形成されたナノポアの大きさを正確に拡大するための方法であって、
0.1ボルト毎ナノメートルを超える電界をナノポアに誘導する電位の値を選択する工程と、
イオン溶液中にあるナノポアに、前記選択された値の電位を印加することによって前記膜の材料を除去し、前記ナノポアを拡大する工程と、
前記電位を前記ナノポアに印加しながら、前記ナノポアを流れる電流を測定する工程と、
前記測定された電流に部分的に基づいて、前記ナノポアの大きさを推定する工程と、
前記測定された電流が閾値を超えたら、前記ナノポアに印加される前記電位を消失させる工程と、を含む、方法。 - 前記ナノポアに印加される前記電位を、前記選択された値未満の値まで下げる工程と、
前記下げられた値の電位を印加しながら、前記ナノポアを流れる電流を測定する工程と、
前記下げられた値の電位を印加しながら、前記電流の測定値に部分的に基づいて、前記ナノポアの大きさを決定する工程と、をさらに含む、請求項10に記載の方法。 - 前記電界が約0.3ボルト毎ナノメートルになるように前記電位の値を選択する工程をさらに含む、請求項10に記載の方法。
- 前記電界が0.10ボルト毎ナノメートルから0.4ボルト毎ナノメートルの範囲に入るように前記電位の値を選択する工程をさらに含む、請求項10に記載の方法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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US201261643651P | 2012-05-07 | 2012-05-07 | |
US61/643,651 | 2012-05-07 | ||
US201361781081P | 2013-03-14 | 2013-03-14 | |
US61/781,081 | 2013-03-14 | ||
PCT/IB2013/000884 WO2013167952A1 (en) | 2012-05-07 | 2013-05-07 | Method for controlling the size of solid-state nanopores |
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JP2015517401A JP2015517401A (ja) | 2015-06-22 |
JP6298450B2 true JP6298450B2 (ja) | 2018-03-20 |
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JP2015510884A Active JP6298450B2 (ja) | 2012-05-07 | 2013-05-07 | 固体ナノポアの大きさを制御するための方法 |
JP2015510885A Active JP6420236B2 (ja) | 2012-05-07 | 2013-05-07 | 高電界を用いたナノポアの作製 |
JP2018108930A Pending JP2018187626A (ja) | 2012-05-07 | 2018-06-06 | 高電界を用いたナノポアの作製 |
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JP2018108930A Pending JP2018187626A (ja) | 2012-05-07 | 2018-06-06 | 高電界を用いたナノポアの作製 |
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US (2) | US9777389B2 (ja) |
EP (2) | EP2847367B1 (ja) |
JP (3) | JP6298450B2 (ja) |
KR (2) | KR102065754B1 (ja) |
CN (2) | CN104662209B (ja) |
AU (2) | AU2013257756B2 (ja) |
BR (2) | BR112014027873B8 (ja) |
CA (2) | CA2872600C (ja) |
ES (2) | ES2629952T3 (ja) |
MX (2) | MX353370B (ja) |
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WO2014144818A2 (en) * | 2013-03-15 | 2014-09-18 | President And Fellows Of Harvard College | Fabrication of nanopores in atomically-thin membranes by ultra-short electrical pulsing |
US10724147B2 (en) | 2013-12-25 | 2020-07-28 | Hitachi, Ltd. | Hole forming method, measuring apparatus and chip set |
JP6209122B2 (ja) * | 2014-04-02 | 2017-10-04 | 株式会社日立ハイテクノロジーズ | 孔形成方法及び測定装置 |
DE102014111984B3 (de) * | 2014-08-21 | 2016-01-21 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Fluidische Gigaohm-Dichtung für Transmembranproteinmessungen |
CN107207246B (zh) | 2014-12-01 | 2019-12-20 | 康奈尔大学 | 具有对齐的纳米级电子元件的含纳米孔的基板及其制备和使用方法 |
KR102414067B1 (ko) | 2014-12-19 | 2022-06-27 | 더 유니버시티 오브 오타와 | 제어된 브레이크다운을 사용한 마이크로유체 채널 어레이 내에서의 나노기공 센서의 통합 |
US10753009B2 (en) | 2015-02-24 | 2020-08-25 | The University Of Ottawa | Localizing nanopore fabrication on a membrane by laser illumination during controlled breakdown |
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