JP6145096B2 - 表面プラズモン共鳴バイオセンサシステム - Google Patents
表面プラズモン共鳴バイオセンサシステム Download PDFInfo
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- JP6145096B2 JP6145096B2 JP2014533242A JP2014533242A JP6145096B2 JP 6145096 B2 JP6145096 B2 JP 6145096B2 JP 2014533242 A JP2014533242 A JP 2014533242A JP 2014533242 A JP2014533242 A JP 2014533242A JP 6145096 B2 JP6145096 B2 JP 6145096B2
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- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 title claims description 81
- 238000001514 detection method Methods 0.000 claims description 70
- 230000003287 optical effect Effects 0.000 claims description 30
- 238000005286 illumination Methods 0.000 claims description 18
- 230000003068 static effect Effects 0.000 claims description 10
- 239000013307 optical fiber Substances 0.000 claims description 6
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000011156 evaluation Methods 0.000 claims description 2
- 230000003993 interaction Effects 0.000 description 16
- 239000012491 analyte Substances 0.000 description 12
- 230000004044 response Effects 0.000 description 10
- 239000003446 ligand Substances 0.000 description 8
- 238000006073 displacement reaction Methods 0.000 description 7
- 238000010494 dissociation reaction Methods 0.000 description 7
- 230000005593 dissociations Effects 0.000 description 7
- 238000010586 diagram Methods 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000010408 film Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000035515 penetration Effects 0.000 description 3
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- 238000004458 analytical method Methods 0.000 description 2
- 230000001427 coherent effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
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- 238000012482 interaction analysis Methods 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
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- 238000012544 monitoring process Methods 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
- G01N21/554—Attenuated total reflection and using surface plasmons detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
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- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
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- Molecular Biology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Nanotechnology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
SPRセンサ表面と、
光の伝播方向と交差するSPRセンサ表面上の線形の検出領域において光のくさび形ビームを方向付けるように配置された照明ユニットと、
反射角が一方の次元に沿ってイメージングされ、検出領域の幅が他方の次元に沿ってイメージングされるように、SPRセンサ表面から2次元光検出器ユニット上に反射された光を方向付けるための検出光学系を備える検出ユニットとを備え、
照明ユニットは、SPRセンサ表面上の2以上の離隔された線形の検出領域において光のくさび形ビームを選択的に方向付けるように配置される、表面プラズモン共鳴(SPR)バイオセンサシステムに関する。
・〜630nmの短波長は、より高い表面感度(プラズモン電界のより短い透過深度、より小さい検出容量)をもたらす。
・〜760−820nmの、近赤外(NIR)範囲のより長い波長は、プラズモン電界のより深い透過深度、より大きい検出容量にわたり平均化された感度をもたらす。
・〜≧1.3μmのIR範囲の波長はさらに、細胞膜及び類似する生物学的構造の検出に適した範囲まで、前記の浸透深度を増大させる。
Claims (10)
- 表面プラズモン共鳴(SPR)バイオセンサシステムであって、当該SPRバイオセンサシステムが、
SPRセンサ表面(1)と、
光の伝播方向と交差するSPRセンサ表面(1)上の線形検出領域(9)にくさび形光ビーム(6)を方向付けるように配置された照明ユニット(7)と、
反射角が列として定義される一方の次元に沿ってイメージングされ、検出領域(9)の幅が行として定義される他方の次元に沿ってイメージングされるように、SPRセンサ表面(1)から2次元光検出器ユニット(22)上に反射光を方向付けるための検出光学系を備える検出ユニット(11)と
を備えており、前記照明ユニット(7)が、光の伝播方向と交差するSPRセンサ表面(1)上の2以上の離隔した線形検出領域(9a,9b)にくさび形光ビーム(6)を選択的に方向付けるように配置されており、これらの検出領域(9a,9b)が複数の検出スポット(13)を含んでいて、2次元光検出器ユニット(22)が複数の列(28)を含んでおり、各々の検出領域(9a,9b)の検出スポット(13)が2次元光検出ユニット(22)の同じ列にイメージングされる、表面プラズモン共鳴(SPR)バイオセンサシステム。 - 前記照明ユニット(7)が、静的照明光学系と、伝播方向と交差する方向に離隔した2以上の光源とを備える、請求項1記載のSPRバイオセンサシステム。
- 前記光源が、各々のくさび形光ビーム(6)がSPRセンサ表面(1)上の対応する線形検出領域(9)に集束されるように、伝播方向に距離を隔てている、請求項2記載のSPRバイオセンサシステム。
- 前記2以上の光源が、2以上の近接して取り付けられたLED、ELED、SLD又はレーザダイオードで構成される、請求項2又は請求項3記載のSPRバイオセンサシステム。
- 前記2以上の光源が、2以上の近接して取り付けられた光ファイバ、導波路、又は導波管で構成される、請求項2又は請求項3記載のSPRバイオセンサシステム。
- 2以上の光源(LED、SLD、ELED、レーザダイオード)が連続的に光を放射し、それらと光ファイバ、導波路又は導波管との光結合が、2次元光検出器ユニット(22)からの読み出しと同期して適切な頻度で光の通過をオン及びオフに交互に切り替えるための手段を含む、請求項5記載のSPRバイオセンサシステム。
- 前記2以上の光源が、2以上のLED、ELED、SLD又はレーザダイオードで構成され、各光源から直接或いは各光源のビームコリメータ、ビームコレクタ又はビームコンデンサを介して入来するビームが、くさび形ビームを形成する静的照明光学系の前に光路に配置されたビーム結合装置の使用によって結合される、請求項2又は請求項3記載のSPRバイオセンサシステム。
- 前記照明ユニット(7)が、静的照明光学系と、光源と、伝播方向と交差する方向に光源からの光ビームを選択的に変換するための光ビーム変換ユニットとを備える、請求項1記載のSPRバイオセンサシステム。
- 2以上の離隔した線形検出領域(9)から反射した光のSPR曲線の結合された読取り値から相対的なSPR角度シフト(複数反射率の降下)を測定するように配置されたSPR評価ユニットを備える、請求項1乃至請求項8のいずれか1項記載のSPRバイオセンサシステム。
- 2以上の離隔した線形検出領域(9)の各々に沿って2以上の検出スポット(13)を備える、請求項1乃至請求項9のいずれか1項記載のSPRバイオセンサシステム。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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SE1150890-0 | 2011-09-28 | ||
SE1150890 | 2011-09-28 | ||
PCT/SE2012/051022 WO2013048318A1 (en) | 2011-09-28 | 2012-09-27 | Surface plasmon resonance biosensor system |
Publications (3)
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JP2014528088A JP2014528088A (ja) | 2014-10-23 |
JP2014528088A5 JP2014528088A5 (ja) | 2015-11-12 |
JP6145096B2 true JP6145096B2 (ja) | 2017-06-07 |
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JP2014533242A Active JP6145096B2 (ja) | 2011-09-28 | 2012-09-27 | 表面プラズモン共鳴バイオセンサシステム |
Country Status (4)
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US (1) | US10768108B2 (ja) |
EP (1) | EP2761274A4 (ja) |
JP (1) | JP6145096B2 (ja) |
WO (1) | WO2013048318A1 (ja) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
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US10330676B2 (en) | 2014-11-12 | 2019-06-25 | Technische Universiteit Eindhoven | Plasmonic biosensor based on molecular conformation |
CN107356770A (zh) * | 2017-05-26 | 2017-11-17 | 中国政法大学 | 一种全自动表面等离子体共振检测仪 |
JP6541921B1 (ja) * | 2018-03-15 | 2019-07-10 | 三菱電機株式会社 | 生体物質測定装置 |
CN111919106B (zh) * | 2018-03-15 | 2023-06-02 | 三菱电机株式会社 | 生物物质测定装置 |
US11231365B2 (en) * | 2019-07-08 | 2022-01-25 | Hanwha Systems Co., Ltd. | Apparatus and method for infrared imaging |
CN114152569A (zh) * | 2021-11-17 | 2022-03-08 | 北京英柏生物科技有限公司 | 一种基于spr角谱的成像光学*** |
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US5917607A (en) | 1996-04-25 | 1999-06-29 | Fuji Photo Film Co., Ltd. | Surface plasmon sensor for multiple channel analysis |
JP4043538B2 (ja) | 1996-04-25 | 2008-02-06 | 富士フイルム株式会社 | 表面プラズモンセンサー |
GB9623820D0 (en) | 1996-11-16 | 1997-01-08 | Secr Defence | Surface plasma resonance sensor |
JP3399804B2 (ja) * | 1997-02-26 | 2003-04-21 | 富士写真フイルム株式会社 | 表面プラズモンセンサー |
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-
2012
- 2012-09-27 WO PCT/SE2012/051022 patent/WO2013048318A1/en active Application Filing
- 2012-09-27 JP JP2014533242A patent/JP6145096B2/ja active Active
- 2012-09-27 US US14/342,868 patent/US10768108B2/en active Active
- 2012-09-27 EP EP12836996.4A patent/EP2761274A4/en not_active Withdrawn
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Publication number | Publication date |
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JP2014528088A (ja) | 2014-10-23 |
WO2013048318A1 (en) | 2013-04-04 |
US20140227136A1 (en) | 2014-08-14 |
EP2761274A1 (en) | 2014-08-06 |
EP2761274A4 (en) | 2015-06-03 |
US10768108B2 (en) | 2020-09-08 |
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