JP5559188B2 - フィトケミカルとして使用するための植物抽出物 - Google Patents
フィトケミカルとして使用するための植物抽出物 Download PDFInfo
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- JP5559188B2 JP5559188B2 JP2011536757A JP2011536757A JP5559188B2 JP 5559188 B2 JP5559188 B2 JP 5559188B2 JP 2011536757 A JP2011536757 A JP 2011536757A JP 2011536757 A JP2011536757 A JP 2011536757A JP 5559188 B2 JP5559188 B2 JP 5559188B2
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- grape
- extract
- water
- vitis vinifera
- mildew
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- 235000017807 phytochemicals Nutrition 0.000 title description 2
- 229930000223 plant secondary metabolite Natural products 0.000 title description 2
- 239000000419 plant extract Substances 0.000 title 1
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 50
- 240000006365 Vitis vinifera Species 0.000 claims description 44
- 241000196324 Embryophyta Species 0.000 claims description 33
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 29
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 29
- 239000000284 extract Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 235000002532 grape seed extract Nutrition 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 241000510928 Erysiphe necator Species 0.000 claims description 10
- 241000233679 Peronosporaceae Species 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 230000000855 fungicidal effect Effects 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 6
- 241001281803 Plasmopara viticola Species 0.000 claims description 5
- 230000002265 prevention Effects 0.000 claims description 5
- 239000004480 active ingredient Substances 0.000 claims description 4
- 229920005989 resin Polymers 0.000 claims description 4
- 239000011347 resin Substances 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 3
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- 229940087559 grape seed Drugs 0.000 claims description 2
- 238000001179 sorption measurement Methods 0.000 claims description 2
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- 244000052769 pathogen Species 0.000 description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
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- 241000233654 Oomycetes Species 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 5
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- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 240000003768 Solanum lycopersicum Species 0.000 description 4
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- 238000011081 inoculation Methods 0.000 description 4
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 3
- 241000221785 Erysiphales Species 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
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- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 3
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- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 2
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
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- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 241000722133 Tilletia Species 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
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- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 2
- 235000012734 epicatechin Nutrition 0.000 description 2
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- ZXVONLUNISGICL-UHFFFAOYSA-N 4,6-dinitro-o-cresol Chemical group CC1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O ZXVONLUNISGICL-UHFFFAOYSA-N 0.000 description 1
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- 241000219193 Brassicaceae Species 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 241001529387 Colletotrichum gloeosporioides Species 0.000 description 1
- 241001120669 Colletotrichum lindemuthianum Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 239000005749 Copper compound Substances 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- WLLGXSLBOPFWQV-UHFFFAOYSA-N MGK 264 Chemical compound C1=CC2CC1C1C2C(=O)N(CC(CC)CCCC)C1=O WLLGXSLBOPFWQV-UHFFFAOYSA-N 0.000 description 1
- 244000081841 Malus domestica Species 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101100412856 Mus musculus Rhod gene Proteins 0.000 description 1
- 241000498271 Necator Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000736122 Parastagonospora nodorum Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 241000233629 Phytophthora parasitica Species 0.000 description 1
- 241000233610 Plasmopara halstedii Species 0.000 description 1
- 241000896203 Podosphaera pannosa Species 0.000 description 1
- 241000221300 Puccinia Species 0.000 description 1
- 241000221301 Puccinia graminis Species 0.000 description 1
- 241001123569 Puccinia recondita Species 0.000 description 1
- 240000001987 Pyrus communis Species 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 241000219492 Quercus Species 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
- 241001533598 Septoria Species 0.000 description 1
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- 235000004433 Simmondsia californica Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000003639 Student–Newman–Keuls (SNK) method Methods 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 241000228448 Taphrina deformans Species 0.000 description 1
- 241000722093 Tilletia caries Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241000509513 Ustilago hordei Species 0.000 description 1
- 244000301083 Ustilago maydis Species 0.000 description 1
- 235000015919 Ustilago maydis Nutrition 0.000 description 1
- 241000233791 Ustilago tritici Species 0.000 description 1
- 241000317942 Venturia <ichneumonid wasp> Species 0.000 description 1
- 241001006642 Venturia pyrina Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 241001360088 Zymoseptoria tritici Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- QSIYTPCKNAPAJY-UHFFFAOYSA-N aluminum;ethoxy-oxido-oxophosphanium;2-(trichloromethylsulfanyl)isoindole-1,3-dione Chemical compound [Al+3].CCO[P+]([O-])=O.CCO[P+]([O-])=O.CCO[P+]([O-])=O.C1=CC=C2C(=O)N(SC(Cl)(Cl)Cl)C(=O)C2=C1 QSIYTPCKNAPAJY-UHFFFAOYSA-N 0.000 description 1
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- 125000003785 benzimidazolyl group Chemical class N1=C(NC2=C1C=CC=C2)* 0.000 description 1
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- 230000015572 biosynthetic process Effects 0.000 description 1
- OIPMQULDKWSNGX-UHFFFAOYSA-N bis[[ethoxy(oxo)phosphaniumyl]oxy]alumanyloxy-ethoxy-oxophosphanium Chemical compound [Al+3].CCO[P+]([O-])=O.CCO[P+]([O-])=O.CCO[P+]([O-])=O OIPMQULDKWSNGX-UHFFFAOYSA-N 0.000 description 1
- DKVNPHBNOWQYFE-UHFFFAOYSA-N carbamodithioic acid Chemical compound NC(S)=S DKVNPHBNOWQYFE-UHFFFAOYSA-N 0.000 description 1
- -1 carbanilate Chemical compound 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
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- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- HKIOYBQGHSTUDB-UHFFFAOYSA-N folpet Chemical compound C1=CC=C2C(=O)N(SC(Cl)(Cl)Cl)C(=O)C2=C1 HKIOYBQGHSTUDB-UHFFFAOYSA-N 0.000 description 1
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 description 1
- 229940087603 grape seed extract Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 229940119170 jojoba wax Drugs 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 235000021243 milk fat Nutrition 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000002018 neem oil Substances 0.000 description 1
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- 230000007918 pathogenicity Effects 0.000 description 1
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- 244000000003 plant pathogen Species 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
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- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
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- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
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- 239000011593 sulfur Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000011514 vinification Methods 0.000 description 1
- 239000001717 vitis vinifera seed extract Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N2300/00—Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S424/00—Drug, bio-affecting and body treating compositions
- Y10S424/10—Insect repellent
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Description
真菌、糸状菌、卵菌、細菌およびウイルスなどの病原体によって引き起こされるいくつかの植物病害が知られている。これらの病害の多くは、通常、その発症および拡散に好都合な気候条件と関係して劇的な結果をもたらし、特に病害が流行状態を呈する場合に、農業経済に深く、時に破滅的な影響をもたらしうる作物の収量の減少を引き起こす。
ブドウ(Vitis vinifera)の種子または種子を含む搾りかすの抽出物が、植物病原体、特に真菌および卵菌の防除に有効であることが分かった。
Erysiphe necator=Uncinula necator(ブドウ)
Erysiphe graminis(穀物)
Podosphaera leucotricha(樹木作物、果樹)
Sphaerotheca pannosa(バラ植物)
2−べと病
Plasmopara viticola(ブドウ)
Plasmopara helianthi(ヒマワリ)
Phytophthora infestans(ジャガイモ)
Phytophthora parasitica(トマト)。
Venturia inaequalis(リンゴの木)
Venturia pirina(ナシの木)
4−モモ縮葉病
Taphrina deformans。
Septoria nodorumおよびSeptoria tritici(穀物)
Septoria菌種(セイヨウアブラナ)
6−さび病
Puccinia graminis、Puccinia reconditaおよびPuccinia striiformis(穀物)。
Ustilago tritici(コムギ)
Ustilago maydis(トウモロコシ)
Ustilago hordei(オオムギ)
8−なまぐさ黒穂病
Tilletia tritici、Tilletia cariesおよびTilletia foetida(穀物)。
Phoma lingam(アブラナ科植物、セイヨウアブラナ)
Rhizoctonia solani(ジャガイモ)
Sclerotinia sclerotiorum(トマト)
10−炭疸病
Colletotrichum gloeosporioides(エンドウ)
Colletotrichum lindemuthianum(インゲン)
11−灰色かび病
Botrytis cinerea(ブドウ)。
− 熱水によるブドウ種子の抽出、
− 水不溶性画分の除去、
− 吸着樹脂カラムでの精製、
− 濃縮、
− 噴霧乾燥
を含む方法によって得ることができることも分かった。
乾燥ブドウ種子1000gを、攪拌器を備えたジャケットのついた静止型パーコレーターまたはジャケットのついた反応器中で4時間、75℃の熱水で覆う。
植物
ブドウ(Vitis vinifera)の感受性品種であるシャルドネ種を使用して、べと病に対する殺真菌剤の効果を調査した。植物は種子から栽培し(0.1〜1ポット当たり1植物)、生育箱(日中23℃/夜19℃、100〜120μE・m−2・s−1、1日当たり16時間の光)に入れた。週に2度、0.1リットルの0.2g/lのN−P−K肥料(20−20−20)を実生に与えた。VITIVACの生物活性を調査するために、少なくとも4枚の完全展開葉を有する8週齢の実生を使用した。
フランスのシャンパーニュ地方に位置するブドウ園の感染植物から2007年に得た、ブドウべと病菌の分離株Pv1を、「シャルドネ」の切断した表面を殺菌した葉の上に保持した。その葉をペトリ皿に置き、生育箱(日中23℃/夜19℃、100〜120μE・m−2・s−1、1日当たり16時間の光)に入れた。接種菌液は、接種後8〜12日に、新たに胞子形成している葉から得た。胞子嚢を4℃の蒸留水に懸濁し、血球計算器を用いて5×105胞子嚢・水ml−1の濃度に調整した。
例1に記載のように調製した天然生成物(VITIVAC)を、実験の間を通して、25g/lの割合で使用した。VITIVACの効果は、製剤化された製品4.0kg/haの割合で使用したホセチル(Fosetyl)−Al(500g/kg)+ホルペル(Folpel)(250g/kg)の基準殺真菌剤混合物(Mikal Flash WG、Bayer CropScience)の効果と比較した。
処理または未処理(対照)の葉それぞれからコルクボーラーで10枚のリーフディスク(それぞれ直径10mm)を切り取り、背軸側を上にして、滅菌蒸留水4mlで湿らせた濾紙を含む直径9cmのプラスチックペトリ皿に置いた。接種菌液(5×105胞子嚢・ml−1)1滴(20μl)を置くことによって、ディスクに接種した。接種後、10枚のリーフディスクを含むペトリ皿を、暗所で24時間、19℃でインキュベートし、次いで、発病のために生育箱(日中23℃/夜19℃、100〜120μE・m−2・s−1、1日当たり16時間の光)中に保った。実験の最後に、ペトリ皿を暗所で24時間、19℃に保ち、胞子形成を誘発した。
例3
ブドウ(Vitis vinifera)抽出物 250g
Soquabiol(登録商標) 200ml
脱イオン水 100l
例4
ブドウ抽出物 500g
Sticman(登録商標) 40ml
脱イオン水 100l
Claims (5)
- 有効成分としてブドウ(Vitis vinifera)の種子または種子を含む搾りかすの抽出物を含む、ブドウべと病(Plasmopara viticola)またはブドウうどんこ病(Erysiphe necator)予防用または治療用組成物。
- 前記抽出物が水/エタノールまたは水抽出物である、請求項1に記載の組成物。
- 殺真菌活性を有する他の有効成分をさらに含む、請求項1または2に記載の組成物。
- 有効量のブドウ(Vitis vinifera)種子抽出物を処置する植物に施用することを含む、ブドウべと病(Plasmopara viticola)またはブドウうどんこ病(Erysiphe necator)を予防または治療する方法。
- 熱水によるブドウ(Vitis vinifera)種子の抽出、
水不溶性画分の除去、
吸着樹脂カラムでの精製、
濃縮および微粒化、
噴霧乾燥
を含む方法によって得ることができる、ブドウべと病(Plasmopara viticola)またはブドウうどんこ病(Erysiphe necator)を予防または治療するためのブドウ(Vitis vinifera)種子の抽出物。
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JP5832201B2 (ja) * | 2011-08-29 | 2015-12-16 | 攝津製油株式会社 | 殺ノロウイルス組成物 |
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SI2189062T1 (sl) | 2013-11-29 |
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ES2431338T3 (es) | 2013-11-26 |
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IL213081A0 (en) | 2011-07-31 |
EP2189062A1 (en) | 2010-05-26 |
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DK2189062T3 (da) | 2013-08-19 |
JP2012509850A (ja) | 2012-04-26 |
AU2009319417B2 (en) | 2014-02-06 |
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HK1140914A1 (en) | 2010-10-29 |
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CN102223802A (zh) | 2011-10-19 |
RU2535367C2 (ru) | 2014-12-10 |
US9456610B2 (en) | 2016-10-04 |
PT2189062E (pt) | 2013-10-22 |
CN102223802B (zh) | 2015-02-25 |
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