JP4619224B2 - Rotational analysis device - Google Patents

Rotational analysis device Download PDF

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JP4619224B2
JP4619224B2 JP2005216736A JP2005216736A JP4619224B2 JP 4619224 B2 JP4619224 B2 JP 4619224B2 JP 2005216736 A JP2005216736 A JP 2005216736A JP 2005216736 A JP2005216736 A JP 2005216736A JP 4619224 B2 JP4619224 B2 JP 4619224B2
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JP2007033225A (en
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博司 佐伯
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Panasonic Corp
Panasonic Holdings Corp
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Matsushita Electric Industrial Co Ltd
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本発明は、生物学的流体を光学的に分析する回転分析デバイスに関するものであり、より詳細には、光学的分析装置で生物学的流体の測定に使用する回転分析デバイスにおける生物学的流体の計量に関する。   The present invention relates to a rotational analysis device for optically analyzing a biological fluid, and more particularly, to a biological fluid in a rotational analysis device used for measurement of biological fluid in an optical analyzer. Concerning weighing.

従来、生物学的流体を光学的に分析する方法として液体流路を形成したマイクロデバイスを用いて分析する方法が知られている。マイクロデバイスは回転装置を使って流体の制御をすることが可能であり、遠心力を利用して試料の計量、細胞質材料の分離、分離された流体の移送分配等を行うことができるため、種々の生物化学的な分析を行うことが可能である。   2. Description of the Related Art Conventionally, as a method for optically analyzing a biological fluid, a method for analyzing using a microdevice having a liquid flow path is known. Microdevices can control fluids using a rotating device, and use centrifugal force to measure samples, separate cytoplasmic materials, transfer and distribute separated fluids, etc. It is possible to perform biochemical analysis.

遠心力を利用して試料を計量する方法としては、図７に示すように中心から周縁に向けて分析前に希釈すべき液体を収容する中央収容部７１と、計量室７２及び溢流室７３と、混合室７４と、測定セル７５とを備え、計量室７２が溢流室７３とほぼ平行に配置され、且つ供給口７６及び溢流口７７以外に供給口７６と対向する計量室壁面に設けられる開口７８を有し、この開口が常時開放されると共に、供給口７６及び溢流口７７より遥かに小さい断面を有することを特徴とする回転分析デバイスがあり、このような構成にすることで計量室７２の充填が高速で実施され、且つその溢流が即刻除去される。液体は計量室７２が液体で満たされ始めるとすぐにこの室から流出し始める。そのため、流入口断面積対流出口断面積の比の関数たる供給時間対流出口からの流出時間の比を好きなだけ小さくすることができることから、測定に正確さが与えられる（特許文献１）。   As a method for measuring the sample using the centrifugal force, as shown in FIG. 7, a central storage portion 71 for storing a liquid to be diluted before analysis from the center to the periphery, a measurement chamber 72 and an overflow chamber 73. And a mixing chamber 74 and a measuring cell 75, the measuring chamber 72 is disposed substantially parallel to the overflow chamber 73, and is provided on the wall of the measuring chamber facing the supply port 76 in addition to the supply port 76 and the overflow port 77. There is a rotational analysis device characterized in that it has an opening 78 provided, this opening is always open and has a cross section much smaller than the supply port 76 and the overflow port 77, and this configuration Thus, the filling of the measuring chamber 72 is performed at high speed, and the overflow is immediately removed. Liquid begins to flow out of this chamber as soon as the metering chamber 72 begins to fill with liquid. Therefore, since the ratio of the supply time to the outflow time from the outflow outlet as a function of the ratio of the inflow cross-sectional area to the outflow outlet cross-sectional area can be reduced as much as desired, accuracy is given to measurement (Patent Document 1).

また、図８に示すように大型流体室８１と、大型流体室８１に連結されると共に大型流体室８１に対して半径方向外方に配置された計量室８２と、計量室８２に連結された溢流室８３と、計量室８２に対して半径方向外方に配置された受容室８４と、計量室８２から受容室８４に液体を供給するための毛細管連結手段８５とを有する回転分析デバイスがあり、毛細管連結手段８５は毛細管構造を有するサイフォン８６を含み、サイフォン８６の肘状屈曲部分が、回転分析デバイスの中心から、計量室８２の半径方向再内方点と実質的に同じ距離になるように位置付けられることで、回転分析デバイスの回転中は毛細管力が遠心力に比べて小さいため、液体／空気の界面は回転分析デバイスの軸線と同じ軸線を有し、且つ回転分析デバイスの中心から計量室８２の半径方向再内方点までの距離に等しい長さの半径を持つ回転円筒体の形状と合致して計量室８２は充填され、過剰な液は溢流室８３に流れ込む。回転分析デバイスを止めると、計量室８２内に充填された液が、毛細管力で毛細管連結手段８５に流入し、再度回転させることでサイフォンが始動し、計量室８２内に存在する液は受容室８４に排出される（特許文献２）。
特開昭６１−１６７４６９号公報 特表平５−５０８７０９号公報 Further, as shown in FIG. 8, a large fluid chamber 81, a measuring chamber 82 connected to the large fluid chamber 81 and arranged radially outward with respect to the large fluid chamber 81, and connected to the measuring chamber 82. A rotational analysis device having an overflow chamber 83, a receiving chamber 84 disposed radially outward with respect to the measuring chamber 82, and capillary connection means 85 for supplying liquid from the measuring chamber 82 to the receiving chamber 84. Yes, the capillary connection means 85 includes a siphon 86 having a capillary structure, and the elbow bend of the siphon 86 is at substantially the same distance from the center of the rotational analysis device as the radial re-inward point of the metering chamber 82. The liquid / air interface has the same axis as the axis of the rotational analysis device, and the rotational analysis device has the same axis as the capillary force during rotation of the rotational analysis device is small compared to the centrifugal force. Consistent with the shape of the rotating cylinder having a radial distance equal to the length of up to radially re inward point of the metering chamber 82 is measuring chamber 82 is filled from the excess liquid flows into the overflow chamber 83. When the rotation analysis device is stopped, the liquid filled in the measuring chamber 82 flows into the capillary connecting means 85 by capillary force and is rotated again to start the siphon, and the liquid existing in the measuring chamber 82 is received in the receiving chamber. 84 (Patent Document 2).
JP 61-167469 A Japanese National Patent Publication No. 5-508709

しかしながら、前記従来の構成では、回転分析デバイスが回転中の時は、遠心力が液体と計量室壁面との間に働く表面張力より大きいため、溢流口の開口位置で液面が釣り合って所定の量を計量できているが、次工程に移るために回転を減速あるいは停止させた場合に、液体は遠心力から開放されると同時に液体と溢流口壁面の界面で表面張力が働き出し、その表面張力によって液体は溢流口の壁面を伝って溢流室に流出してしまい、精密な計量ができていなかった。また、液体の物性値の違いによって流出する量がばらつくため、分析する液体ごとに計量室の大きさを変える必要があった。   However, in the conventional configuration, when the rotational analysis device is rotating, since the centrifugal force is larger than the surface tension acting between the liquid and the wall surface of the measuring chamber, the liquid level is balanced at the opening position of the overflow port, and the predetermined value is obtained. However, when the rotation is decelerated or stopped to move to the next process, the liquid is released from the centrifugal force, and at the same time, the surface tension starts working at the interface between the liquid and the overflow wall, Due to the surface tension, the liquid flowed along the wall of the overflow port into the overflow chamber, and precise measurement was not possible. In addition, since the amount of liquid flowing out varies depending on the physical property values of the liquid, it is necessary to change the size of the measuring chamber for each liquid to be analyzed.

本発明は、前記従来の課題を解決するもので、液体の計量を精度よく行うことのできる回転分析デバイスを提供することを目的とする。   An object of the present invention is to solve the above-mentioned conventional problems, and to provide a rotational analysis device capable of accurately measuring a liquid.

前記従来の課題を解決するために、本発明の回転分析デバイスは、分析するために必要な量の試料液を注入／収容するための液体収容室と、前記液体収容室に連結通路によって連結されるとともに前記液体収容室に対して半径方向外方に配置された前記試料液を一定量保持する計量室と、前記計量室に連結され当該計量室の容量よりも過剰な容量の試料液を受容するための溢流室を有する回転分析デバイスであって、前記計量室が少なくとも回転分析デバイスの半径方向内側に位置する壁面と回転分析デバイスの半径方向外側に位置する壁面とを有し、前記連結通路を介して液体収容室から前記試料液が流入する流入口と、前記計量室から前記溢流室への溢流口が、前記計量室の回転分析デバイスの半径方向内側に位置する壁面に離間して設けられ、前記計量室の溢流口からの試料液が流れ込む前記溢流室の流入口は、前記計量室の溢流口より半径方向内方に配置され、前記計量室の溢流口と毛細管通路で連結するとともに、前記計量室を構成する壁面のうち回転分析デバイスの半径方向内側に位置する前記壁面は、当該計量室の前記流入口から当該計量室の前記溢流口へ向かうに従って前記半径方向位置が回転中心へ近接するように形成されていることを特徴とする。 In order to solve the above-mentioned conventional problems, the rotational analysis device of the present invention is connected to a liquid storage chamber for injecting / accommodating a necessary amount of sample liquid for analysis, and to the liquid storage chamber by a connecting passage. And a measuring chamber for holding a certain amount of the sample liquid disposed radially outward with respect to the liquid storage chamber, and receiving a sample liquid having a volume exceeding the capacity of the measuring chamber connected to the measuring chamber. A rotating analysis device having an overflow chamber for performing the measurement , wherein the weighing chamber has at least a wall surface located radially inside the rotational analysis device and a wall surface located radially outside the rotational analysis device, and the connection An inlet through which the sample liquid flows from the liquid storage chamber through the passage and an overflow port from the measurement chamber to the overflow chamber are separated from a wall surface located radially inward of the rotational analysis device of the measurement chamber. Set up Is the inlet of the overflow chamber of the sample solution flows from the overflow port of the metering chamber is located radially inward from the overflow port of the metering chamber, the overflow opening and the capillary channel of the metering chamber And the wall surface located on the radially inner side of the rotational analysis device among the wall surfaces constituting the measurement chamber is in the radial direction from the inlet of the measurement chamber toward the overflow port of the measurement chamber. The position is formed so as to be close to the center of rotation .

また、さらに本発明は、前記計量室で計量された試料液を移送して該試料液の吸光度を測定するための測定セルと、前記計量室と前記測定セルをつなぐ連結通路の間に前記計量室の測定セル側出口より前記半径方向の外方に毛細管バルブを設けたことを特徴とする。 Still present invention, the weighing and measuring cell for measuring the absorbance of the sample solution and transferring the sample liquid that has been weighed in the weighing chamber, between the connecting passage connecting the measuring cell and the measuring chamber A capillary valve is provided outward from the measurement cell side outlet of the chamber in the radial direction.

本発明の回転分析デバイスによれば、回転分析デバイスを回転させて計量室に保持させた液体を、次工程に移るために回転を減速あるいは停止させても、計量室と溢流室を毛細管で連結させることで、液体はその毛細管内にトラップされて溢流室に流出しないため、正確な計量を行うことができる。また、表面張力の異なる液体も計量後の溢流室への流出が無くなるため、同じ量だけ計量することができる。   According to the rotational analysis device of the present invention, even if the liquid held in the measurement chamber by rotating the rotational analysis device is decelerated or stopped in order to move to the next step, the measurement chamber and the overflow chamber are separated by a capillary. By connecting, the liquid is trapped in the capillary and does not flow out into the overflow chamber, so that accurate measurement can be performed. In addition, liquids having different surface tensions can be measured by the same amount because there is no outflow to the overflow chamber after measurement.

以下に、本発明の回転分析デバイスの実施の形態を図面とともに詳細に説明する。   Embodiments of the rotational analysis device of the present invention will be described below in detail with reference to the drawings.

図１は、本発明の第１の実施例における回転分析デバイスの構成を示す模式図である。また、図２は第１の実施例における回転分析デバイスのマイクロチャネルが形成された基板を示す斜視図である。   FIG. 1 is a schematic diagram showing the configuration of a rotational analysis device according to the first embodiment of the present invention. FIG. 2 is a perspective view showing the substrate on which the microchannel of the rotational analysis device in the first embodiment is formed.

図１において、本発明の回転分析デバイスは、マイクロチャネル４ａ、４ｂを有する基板１と、平坦な基板２と、接着層３で構成されている。   In FIG. 1, the rotational analysis device of the present invention includes a substrate 1 having microchannels 4 a and 4 b, a flat substrate 2, and an adhesive layer 3.

基板１に形成されるマイクロチャネル４ａ、４ｂは、図３に示すような凹凸のあるマイクロチャネルパターンを射出成形により作製しており、分析する試料液を回転分析デバイスに注入し、遠心力と毛細管力を利用して流体移動をさせることが可能となっている。   The microchannels 4a and 4b formed on the substrate 1 are produced by injection-molding a microchannel pattern having projections and depressions as shown in FIG. 3, and a sample solution to be analyzed is injected into a rotational analysis device, and centrifugal force and capillary tube are injected. It is possible to move the fluid using force.

本発明では、マイクロチャネル４ａに透過光を照射して、検査すべき試料液と試薬の反応状態を光学的に分析する。測定時には、マイクロチャネル４ａ内に試料液と試薬とを反応させた反応溶液５が充填され、反応溶液５は試料液と試薬の反応の割合で吸光度が変化するため、光源部６からマイクロチャネル４ａに透過光を照射し、受光部７にてその透過光の光量を測定することで、反応溶液５を透過した光量の変化を測定することができるため、反応状態を分析することができる。   In the present invention, the microchannel 4a is irradiated with transmitted light to optically analyze the reaction state between the sample liquid to be examined and the reagent. At the time of measurement, the reaction solution 5 obtained by reacting the sample solution and the reagent is filled in the microchannel 4a, and the absorbance of the reaction solution 5 changes depending on the reaction rate between the sample solution and the reagent. Since the change in the amount of light transmitted through the reaction solution 5 can be measured by irradiating the light with transmitted light and measuring the amount of the transmitted light at the light receiving unit 7, the reaction state can be analyzed.

本発明では、基板１および基板２の厚みは、１ｍｍ〜５ｍｍで形成しているが、特に制限は無く、マイクロチャネル４ａ、４ｂを形成可能な厚みであればよい。基板１および基板２の形状についても特に限定する必要が無く、用途目的に応じた形状、例えば、シート状、板状、棒状、その他複雑な形状の成形物などの形状が可能である。   In this invention, although the thickness of the board | substrate 1 and the board | substrate 2 is formed at 1 mm-5 mm, there is no restriction | limiting in particular, What is necessary is just the thickness which can form microchannel 4a, 4b. The shapes of the substrate 1 and the substrate 2 are not particularly limited, and can be a shape according to the purpose of use, for example, a sheet shape, a plate shape, a rod shape, or other complicated shapes.

本発明では基板１および基板２の材料として、易成形性、高生産性、低価格の面からプラスチックを使用しているが、ガラス、シリコンウェハー、金属、セラミックなど接合できる材料であれば特に制限はない。   In the present invention, plastic is used as the material of the substrate 1 and the substrate 2 from the viewpoint of easy moldability, high productivity, and low cost. There is no.

本発明ではマイクロチャネル４ａ、４ｂを有する基板１および基板２にはマイクロチャネル４ａ、４ｂ内の粘性抵抗を減らし流体移動をしやすくするために親水性処理を行っているが、ガラス等の親水性材料を用いたり、成形時に界面活性剤、親水性ポリマー、シリカゲルの如き親性粉末などの親水化剤を添加させて材料表面に親水性を付与させたりしてもかまわない。親水性処理方法としては、プラズマ、コロナ、オゾン、フッ素等の活性ガスを用いた表面処理方法や界面活性剤による表面処理が挙げられる。ここで、親水性とは水との接触角が９０度未満のことをいい、より好ましくは接触角４０度未満である。   In the present invention, the substrate 1 and the substrate 2 having the microchannels 4a and 4b are subjected to hydrophilic treatment in order to reduce the viscous resistance in the microchannels 4a and 4b and facilitate fluid movement. A material may be used, or a hydrophilic agent such as a surfactant, a hydrophilic polymer, or a hydrophilic powder such as silica gel may be added at the time of molding to impart hydrophilicity to the material surface. Examples of the hydrophilic treatment method include a surface treatment method using an active gas such as plasma, corona, ozone, and fluorine, and a surface treatment with a surfactant. Here, the hydrophilic property means that the contact angle with water is less than 90 degrees, and more preferably, the contact angle is less than 40 degrees.

本発明では、接着剤を用いて基板１と基板２を接合しているが、使用する材料に応じて溶融接合や陽極接合などの接合方法で接合してもかまわない。   In this invention, although the board | substrate 1 and the board | substrate 2 are joined using an adhesive agent, you may join by joining methods, such as a fusion | melting joining and anodic joining, according to the material to be used.

次に上記で説明した本発明の第１の実施例における回転分析デバイスのマイクロチャネル構成および試料液の移送プロセスについて説明する。   Next, the microchannel configuration of the rotational analysis device and the sample liquid transfer process in the first embodiment of the present invention described above will be described.

図３は第１の実施例における回転分析デバイスのマイクロチャネル構成を示す平面図である。また、図４（ａ）〜図４（ｄ）は本発明の第１の実施例における回転分析デバイスの移送プロセスを示す図である。   FIG. 3 is a plan view showing the microchannel configuration of the rotational analysis device in the first embodiment. 4 (a) to 4 (d) are diagrams showing a transfer process of the rotational analysis device in the first embodiment of the present invention.

図２に示すように、本発明の回転分析デバイスのマイクロチャネル構成は、試料液を注入／収容するための液体収容室９と、試料液を一定量計量し保持する計量室１０と、計量室１０の容量よりも過剰な容量の試料液を受容するための溢流室１１と、計量室１０で計量された試料液を移送させて試薬と反応させ、その液の吸光度を測定するための測定セル１２とで構成されている。ここで、本実施例では省略しているが、計量室１０と測定セル１２の間には細胞質を分離するための分離室や、試料液を希釈／混合するための混合室等を設けてもかまわない。   As shown in FIG. 2, the microchannel configuration of the rotational analysis device of the present invention includes a liquid storage chamber 9 for injecting / accommodating sample liquid, a measurement chamber 10 for measuring and holding a predetermined amount of sample liquid, and a measurement chamber. An overflow chamber 11 for receiving a sample liquid of an excess volume of 10 and a measurement for transferring the sample liquid measured in the measuring chamber 10 to react with the reagent and measuring the absorbance of the liquid It consists of a cell 12. Although omitted in the present embodiment, a separation chamber for separating the cytoplasm, a mixing chamber for diluting / mixing the sample liquid, etc. may be provided between the measuring chamber 10 and the measuring cell 12. It doesn't matter.

本発明では、液体試料室９、計量室１０、溢流室１１、測定セル１２の深さを０.３ｍｍ〜２ｍｍで形成しているが、試料液の量や吸光度測定するための条件（光路長、測定波長、試料液の反応濃度、試薬の種類等）によって調整可能である。   In the present invention, the depths of the liquid sample chamber 9, the measurement chamber 10, the overflow chamber 11, and the measurement cell 12 are formed to be 0.3 mm to 2 mm, but the conditions (optical path) for measuring the amount of the sample liquid and the absorbance. Length, measurement wavelength, reaction concentration of sample solution, type of reagent, etc.).

液体収容室９は計量室１０と連結通路１３を介してつながっており、図４（ａ）に示すように注入口８から試料液を注入／収容し、回転分析デバイスを回転させることで、図４（ｂ）に示すように、試料液を計量室１０に移送することができる。   The liquid storage chamber 9 is connected to the measuring chamber 10 via the connecting passage 13, and as shown in FIG. 4A, the sample liquid is injected / stored from the injection port 8, and the rotational analysis device is rotated to As shown in 4 (b), the sample liquid can be transferred to the measuring chamber 10.

本発明では、連結通路１３の深さを液体収容室９や計量室１０と同じ深さにしているが、計量室１０に空気孔を設けることで、深さ５０μｍ〜２００μｍの毛細管形状にすることも可能である。   In the present invention, the depth of the connecting passage 13 is the same as that of the liquid storage chamber 9 and the measuring chamber 10, but by providing an air hole in the measuring chamber 10, a capillary shape having a depth of 50 μm to 200 μm is formed. Is also possible.

計量室１０は、計量室１０より半径方向内方に配置された溢流室１１の流入口１６に、計量室１０の半径方向最内方に位置する溢流口１４から毛細管流路１７を介して連結され、計量室１０の半径方向最外方に位置する場所から連結通路１５を介して測定セル１２に連結されている。溢流室１１には、試料液が流入しやすいように空気孔１８が設けられており、測定セル１２にも、試料液が連結通路１５を流れやすいように空気孔１９が設けられている。   The measuring chamber 10 is connected to the inlet 16 of the overflow chamber 11 arranged radially inward from the measuring chamber 10 and from the overflow port 14 located radially inward of the measuring chamber 10 through the capillary channel 17. And is connected to the measurement cell 12 through a connection passage 15 from a location located radially outward of the weighing chamber 10. The overflow chamber 11 is provided with an air hole 18 so that the sample liquid can easily flow in, and the measurement cell 12 is also provided with an air hole 19 so that the sample liquid can easily flow through the connecting passage 15.

連結通路１５は、回転分析デバイスの回転中心から溢流室１１の流入口１６と、毛細管流路１７の界面までの距離より内方に配置される曲管を備えたサイフォン形状である。本発明では、連結通路１５の幅を０.５ｍｍ〜２ｍｍ、深さを５０μｍ〜２００μｍで形成しているが、毛細管力で連結通路１５内を試料液で充填できるのであれば特に制限はない。   The connection passage 15 has a siphon shape including a curved pipe disposed inward from the distance from the rotation center of the rotational analysis device to the inlet 16 of the overflow chamber 11 and the interface of the capillary channel 17. In the present invention, the width of the connecting passage 15 is 0.5 mm to 2 mm and the depth is 50 μm to 200 μm, but there is no particular limitation as long as the inside of the connecting passage 15 can be filled with the sample solution by capillary force.

このように計量室１０と測定セル１２を連結することで、液体収容室９内に収容された試料液を回転分析デバイスの回転によって計量室１０に移送・充填させても、図４（ｂ）に示すように、連結通路１５内の試料液は、回転分析デバイスの回転中心から溢流室１１の流入口１６と、毛細管流路１７の界面までの半径方向の距離に相当する位置までしか充填されない。計量室１０の充填完了後に回転分析デバイスを停止させると、図４（ｃ）に示すように、連結通路１５内は毛細管力が働き、測定セル１２の入口まで試料液で満たされる。このとき、試料液は測定セル１２の深さが深く、毛細管力が連結通路１５の毛細管力に比べて極めて小さいため、測定セル１２内には流入しない。
連結通路１５が満たされた後、回転分析デバイスを再度回転させることで計量室１０内に保持されている試料液は、図４（ｄ）に示すように、サイフォン効果で測定セルに移送される。 By connecting the measurement chamber 10 and the measurement cell 12 in this way, the sample liquid stored in the liquid storage chamber 9 can be transferred and filled into the measurement chamber 10 by rotation of the rotational analysis device, as shown in FIG. As shown in FIG. 5, the sample liquid in the connection passage 15 is filled only to a position corresponding to the radial distance from the rotation center of the rotational analysis device to the inlet 16 of the overflow chamber 11 and the interface of the capillary channel 17. Not. When the rotational analysis device is stopped after the filling of the measuring chamber 10, a capillary force acts in the connection passage 15 as shown in FIG. 4C, and the sample solution is filled up to the inlet of the measurement cell 12. At this time, the sample liquid does not flow into the measurement cell 12 because the depth of the measurement cell 12 is deep and the capillary force is extremely smaller than the capillary force of the connection passage 15.
After the connection passage 15 is filled, the sample solution held in the measuring chamber 10 by rotating the rotational analysis device again is transferred to the measurement cell by the siphon effect as shown in FIG. 4 (d). .

本発明では、計量室１０を構成する壁面のうち、回転分析デバイスの半径方向内側に位置する壁面の形状が、計量室１０の連結通路１３付近から溢流口１４付近にかけて半径方向内方に入り込むように形成される。即ち、計量室１０を構成する壁面のうち、回転分析デバイスの半径方向内側に位置する壁面は、計量室１０の試料液の流入口から溢流口へ向かうに従って半径方向位置が回転中心へ近接するように形成することで、液体収容室９から試料液を移送させた際に、計量室１０内の空気が溢流口１４に向かって選択的に抜けるため、計量室１０の充填時に空気の混入による試料液の計量ばらつきが少なくなる。   In the present invention, among the wall surfaces constituting the measurement chamber 10, the shape of the wall surface located on the radially inner side of the rotation analysis device enters inward in the radial direction from the vicinity of the connecting passage 13 of the measurement chamber 10 to the vicinity of the overflow port 14. Formed as follows. That is, among the wall surfaces constituting the measuring chamber 10, the wall surface located radially inward of the rotational analysis device has a radial position closer to the center of rotation as it goes from the sample solution inlet to the overflow port in the measuring chamber 10. In this way, when the sample liquid is transferred from the liquid storage chamber 9, air in the measuring chamber 10 is selectively removed toward the overflow port 14, so that air is mixed when the measuring chamber 10 is filled. Dispersion of sample liquid due to is reduced.

本発明では、毛細管流路１７の深さは５０μｍ〜２００μｍで形成されており、このようにすることで、回転分析デバイスの回転中は、溢流室１１の流入口１６と、毛細管流路１７の界面までの半径方向の距離に相当する位置で液面が安定して計量され、回転の減速／停止時には、試料液は毛細管流路１７の毛細管力によって毛細管流路１７内にトラップされているため、溢流室１１への流出を防ぐことができ、精密な計量が可能となる。また、毛細管流路１７内にトラップされている試料液は、次の回転時に遠心力によって計量室１０に戻されるため、計量された試料液を全て次の工程に移送することが可能となる。   In the present invention, the depth of the capillary channel 17 is 50 μm to 200 μm. By doing so, the inlet 16 of the overflow chamber 11 and the capillary channel 17 are rotated during rotation of the rotational analysis device. The liquid level is stably measured at a position corresponding to the radial distance to the interface of the liquid crystal, and the sample liquid is trapped in the capillary channel 17 by the capillary force of the capillary channel 17 when the rotation is decelerated / stopped. Therefore, the outflow to the overflow chamber 11 can be prevented, and precise measurement is possible. Further, since the sample liquid trapped in the capillary channel 17 is returned to the measuring chamber 10 by the centrifugal force at the next rotation, all the measured sample liquid can be transferred to the next step.

図５は、本発明の第２の実施例における回転分析デバイスの構成を示す模式図である。また、図６は第２の実施例における回転分析デバイスのマイクロチャネルが形成された基板を示す斜視図である。   FIG. 5 is a schematic diagram showing the configuration of the rotational analysis device in the second embodiment of the present invention. FIG. 6 is a perspective view showing a substrate on which microchannels of the rotational analysis device in the second embodiment are formed.

図５において、実施例１の構成と異なるところは、計量室１０の溢流口１４と溢流室１１の流入口１６との間に溢流制御室２０を設け、溢流室１１の流入口１６と溢流制御室２０を深さ５０μｍ〜２００μｍの毛細管流路２１を介して連結した点である。   In FIG. 5, the difference from the configuration of the first embodiment is that an overflow control chamber 20 is provided between the overflow port 14 of the measuring chamber 10 and the inlet 16 of the overflow chamber 11, and the inlet of the overflow chamber 11 is provided. 16 and the overflow control chamber 20 are connected through a capillary channel 21 having a depth of 50 μm to 200 μm.

溢流制御室２０は計量室１０および溢流室１１より半径方向内方に配置されており、その深さは、図６に示すように０.３ｍｍ〜２ｍｍで形成されている。このような構成にすることで、溢流室１１に流入した試料液の表面張力が低い場合でも、回転停止時に毛細管流路２１の毛細管力で毛細管流路２１内にトラップされるため、試料液が計量室１０に逆流するのを抑制することが可能となる。   The overflow control chamber 20 is disposed radially inward from the measuring chamber 10 and the overflow chamber 11 and has a depth of 0.3 mm to 2 mm as shown in FIG. With this configuration, even when the surface tension of the sample liquid flowing into the overflow chamber 11 is low, the sample liquid is trapped in the capillary flow path 21 by the capillary force of the capillary flow path 21 when the rotation is stopped. Can be prevented from flowing back into the measuring chamber 10.

同じように、連結通路１５を計量室１０の半径方向最外方より外側の距離に相当する位置と測定セル１２の間に設けた、深さ０.３ｍｍ〜２ｍｍの毛細管バルブ２２を介して計量室１０と測定セル１２を連結することで、測定セル１２に移送させた試料液は、毛細管力の極めて小さい毛細管バルブ２２があるため、毛細管バルブ２２の流出口までしか毛細管力で戻ることができず、計量室１０に逆流するのを抑制することが可能となる。   Similarly, the connecting passage 15 is measured via a capillary valve 22 having a depth of 0.3 mm to 2 mm provided between the measurement cell 12 and a position corresponding to a distance outside the radially outermost position of the measuring chamber 10. By connecting the chamber 10 and the measurement cell 12, the sample liquid transferred to the measurement cell 12 has a capillary valve 22 having a very small capillary force, and can return only to the outlet of the capillary valve 22 by the capillary force. Therefore, it is possible to suppress backflow into the measuring chamber 10.

本発明にかかる回転分析デバイスは、回転分析デバイスを回転させて計量室に保持させた液体を、次工程に移るために回転を減速あるいは停止させても、計量室と溢流室を毛細管で連結させることで、液体はその毛細管内にトラップされて溢流室に流出しないため、正確な計量を行うことができるという効果を有し、光学的分析装置で生物学的流体の測定に使用する回転分析デバイスにおける生物学的流体の計量方法等として有用である。   The rotational analysis device according to the present invention connects the measurement chamber and the overflow chamber with a capillary even when the rotation analysis device is rotated and the liquid held in the measurement chamber is decelerated or stopped to move to the next process. Since the liquid is trapped in the capillary tube and does not flow into the overflow chamber, the liquid can be accurately measured, and the rotation used for measuring the biological fluid in the optical analyzer is effective. This is useful as a method for measuring a biological fluid in an analytical device.

本発明の第１の実施例における回転分析デバイスの構成を示す模式図The schematic diagram which shows the structure of the rotation analysis device in 1st Example of this invention. 第１の実施例における回転分析デバイスのマイクロチャネルが形成された基板を示す斜視図The perspective view which shows the board | substrate with which the microchannel of the rotation analysis device in a 1st Example was formed. 第１の実施例における回転分析デバイスのマイクロチャネル構成を示す平面図The top view which shows the microchannel structure of the rotation analysis device in a 1st Example 第１の実施例における回転分析デバイスの移送プロセスを説明するための図The figure for demonstrating the transfer process of the rotation analysis device in 1st Example 第２の実施例における回転分析デバイスのマイクロチャネル構成を示す平面図The top view which shows the microchannel structure of the rotation analysis device in 2nd Example 第２の実施例における回転分析デバイスのマイクロチャネルが形成された基板を示す斜視図The perspective view which shows the board | substrate with which the microchannel of the rotation analysis device in 2nd Example was formed. 従来例の遠心力を利用して試料の計量を説明するための図Diagram for explaining sample weighing using centrifugal force of conventional example 従来例の他の遠心力を利用して試料の計量を説明するための図Diagram for explaining sample weighing using another centrifugal force of the conventional example

符号の説明Explanation of symbols

１、２ 基板
３ 接着層
４ａ、４ｂ マイクロチャネル
５ 反応溶液
６ 光源部
７ 受光部
８ 注入口
９ 液体収容室
１０ 計量室
１１ 溢流室
１２ 測定セル
１３ 連結通路
１４ 溢流口
１５ 連結通路
１６ 流入口
１７ 毛細管流路
１８、１９ 空気孔
２０ 溢流制御室
２１ 毛細管流路
２２ 毛細管バルブ
７１ 中央収容部
７２ 計量室
７３ 溢流室
７４ 混合室
７５ 測定セル
７６ 供給口
７７ 溢流口
７８ 開口
８１ 大型流体室
８２ 計量室
８３ 溢流室
８４ 受容室
８５ 毛細管連結手段
８６ サイフォン DESCRIPTION OF SYMBOLS 1, 2 Substrate 3 Adhesive layer 4a, 4b Microchannel 5 Reaction solution 6 Light source part 7 Light receiving part 8 Inlet 9 Liquid storage chamber 10 Measurement room 11 Overflow chamber 12 Measurement cell 13 Connection channel 14 Overflow port 15 Connection channel 16 Flow Inlet 17 Capillary flow path 18, 19 Air hole 20 Overflow control chamber 21 Capillary flow path 22 Capillary valve 71 Central housing 72 Measuring chamber 73 Overflow chamber 74 Mixing chamber 75 Measurement cell 76 Supply port 77 Overflow port 78 Opening 81 Large Fluid chamber 82 Measuring chamber 83 Overflow chamber 84 Receiving chamber 85 Capillary connection means 86 Siphon

Claims (4)

分析するために必要な量の試料液を注入／収容するための液体収容室と、
前記液体収容室に連結通路によって連結されるとともに前記液体収容室に対して半径方向外方に配置された前記試料液を一定量保持する計量室と、
前記計量室に連結され当該計量室の容量よりも過剰な容量の試料液を受容するための溢流室を有する回転分析デバイスであって、
前記計量室が少なくとも回転分析デバイスの半径方向内側に位置する壁面と回転分析デバイスの半径方向外側に位置する壁面とを有し、前記連結通路を介して液体収容室から前記試料液が流入する流入口と、前記計量室から前記溢流室への溢流口が、前記計量室の回転分析デバイスの半径方向内側に位置する壁面に離間して設けられ、前記計量室の溢流口からの試料液が流れ込む前記溢流室の流入口は、前記計量室の溢流口より半径方向内方に配置され、前記計量室の溢流口と毛細管通路で連結するとともに、前記計量室を構成する壁面のうち回転分析デバイスの半径方向内側に位置する前記壁面は、当該計量室の前記流入口から当該計量室の前記溢流口へ向かうに従って前記半径方向位置が回転中心へ近接するように形成されている
回転分析デバイス。 A liquid storage chamber for injecting / accommodating the amount of sample liquid required for analysis;
A measuring chamber connected to the liquid storage chamber by a connecting passage and holding a certain amount of the sample liquid disposed radially outward with respect to the liquid storage chamber;
A rotational analysis device connected to the weighing chamber and having an overflow chamber for receiving an excess volume of sample liquid than the volume of the weighing chamber;
The measurement chamber has at least a wall surface located radially inward of the rotational analysis device and a wall surface located radially outward of the rotational analysis device, and the flow of the sample liquid from the liquid storage chamber through the connection passage An inlet and an overflow port from the measuring chamber to the overflow chamber are provided on a wall surface located radially inward of the rotational analysis device of the measuring chamber, and a sample from the overflow port of the measuring chamber is provided. The inlet of the overflow chamber into which the liquid flows is disposed radially inward from the overflow port of the measuring chamber, is connected to the overflow port of the measuring chamber by a capillary passage, and is a wall surface constituting the measuring chamber The wall surface located radially inward of the rotational analysis device is formed so that the radial position is closer to the center of rotation as it goes from the inlet of the measuring chamber to the overflow port of the measuring chamber. <br/> times you are Analysis device. 前記計量室の前記溢流口と前記溢流室の前記流入口を、前記計量室及び前記溢流室より前記半径方向内方に配置された溢流制御室を介して連結したThe overflow port of the measuring chamber and the inlet of the overflow chamber are connected via an overflow control chamber disposed radially inward from the measuring chamber and the overflow chamber.
請求項１記載の回転分析デバイス。The rotation analysis device according to claim 1. 前記計量室で計量された試料液を移送して該試料液の吸光度を測定するための測定セルと、
前記計量室と前記測定セルをつなぐ連結通路の間に前記計量室の測定セル側出口より前記半径方向の外方に毛細管バルブを設けた
請求項１記載の回転分析デバイス。 A measurement cell for transferring the sample solution weighed in the measurement chamber and measuring the absorbance of the sample solution;
A capillary valve is provided between the measuring chamber and the measurement cell side outlet in the radial direction between the connecting passage connecting the measuring chamber and the measuring cell.
The rotation analysis device according to claim 1. 前記毛細管バルブは、前記計量室への逆流を抑制するために毛細管力を前記計量室と前記測定セルをつなぐ連結通路の毛細管力より小さく設定したIn the capillary valve, the capillary force is set to be smaller than the capillary force of the connecting passage connecting the measuring chamber and the measuring cell in order to suppress the backflow to the measuring chamber.
請求項３記載の回転分析デバイス。The rotation analysis device according to claim 3.
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