JP4028865B2 - Separation structure for separating liquid components from a mixed sample in which the sample and additive liquid are mixed - Google Patents

Separation structure for separating liquid components from a mixed sample in which the sample and additive liquid are mixed Download PDF

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JP4028865B2
JP4028865B2 JP2004292421A JP2004292421A JP4028865B2 JP 4028865 B2 JP4028865 B2 JP 4028865B2 JP 2004292421 A JP2004292421 A JP 2004292421A JP 2004292421 A JP2004292421 A JP 2004292421A JP 4028865 B2 JP4028865 B2 JP 4028865B2
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JP2006105770A (en
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眞康 須田
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株式会社ヒポクラテス
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Description

本発明は比較的微量の検体に加え液を加えて混合し、液状成分を分離するための分離構造に係り、主に血液から血漿を分離するために用いられる。   The present invention relates to a separation structure for separating a liquid component by adding a liquid to a relatively small amount of sample and mixing the liquid, and is mainly used for separating plasma from blood.

従来の血液成分分離構造は、血液成分と化学物質を含む溶液とを混合するための手段と、血液成分から主に血漿成分を分離するための分離手段を有し、且つ前記混合するための手段と前記分離手段とを接続するための連結手段を有し、血液成分を自然落下によりフィルターを通して血漿成分を分離するものが存在する。さらに必要に応じて加圧または引圧を生じさせる駆動手段を有し、迅速に血漿成分を分離するものも存在している（例えば、特許文献１参照。）。
特開２００３−１４９２３４号公報 A conventional blood component separation structure has means for mixing a blood component and a solution containing a chemical substance, and means for separating mainly a plasma component from the blood component, and means for mixing And a separation means for connecting the separation means with each other, and a blood component is separated through a filter by natural falling of the blood component. Furthermore, there is a drive unit that generates pressurization or pulling pressure as needed, and quickly separates plasma components (see, for example, Patent Document 1).
JP 2003-149234 A

本発明は検体と加え液を混合した混合検体から液状成分を分離する時間を短縮し、且つ分離時に検体に加圧又は減圧を生じさせないことを課題とする。前述したように、従来の分離構造は血液成分を自然落下によりフィルターを通して血漿成分を分離している。しかし、自然落下による分離は非常に時間がかかるという欠点がある。その欠点を克服するために、加圧または引圧を生じさせる駆動手段を利用して分離時間を短縮するものが存在している。しかし、血液などの検体に加圧又は減圧を生じさせることは、正確な分析等の障害になる虞があり好ましいことではない。そこで本発明は、自然落下を利用せずに迅速に分離することができ、しかも検体に加圧又は減圧をほとんど生じさせない分離構造を提供することを目的とする。   It is an object of the present invention to shorten the time for separating a liquid component from a mixed specimen obtained by mixing a specimen and an added liquid, and to prevent pressurization or decompression of the specimen during separation. As described above, the conventional separation structure separates the plasma component through the filter by the natural fall of the blood component. However, there is a drawback that separation by natural fall is very time consuming. In order to overcome this drawback, there is a technique that uses a driving means for generating pressurization or pulling pressure to shorten the separation time. However, it is not preferable to cause the specimen such as blood to be pressurized or decompressed because it may cause an obstacle to accurate analysis. In view of the above, an object of the present invention is to provide a separation structure that can quickly separate without using natural fall and that hardly causes pressurization or decompression of a specimen.

本発明の請求項１は、検体と加え液を混合した混合検体を入れるための貯留室と、その混合検体から分離した液状成分を入れるための採取室を有し、貯留室と採取室はフィルターを介して連通しており、貯留室及び採取室にそれぞれピストンが装着され、両ピストンは同時に同方向へ移動するように連結装置によって連結されている構成である。   Claim 1 of the present invention has a storage chamber for storing a mixed sample obtained by mixing a sample and an added liquid, and a sampling chamber for storing a liquid component separated from the mixed sample, and the storage chamber and the sampling chamber are filters. The piston is attached to each of the storage chamber and the sampling chamber, and both the pistons are connected by a connecting device so as to move in the same direction at the same time.

請求項２は、採取室に入った液状成分が逆流して採取室から出ることを防止するための手段を備えている要素が請求項１に限定的に付加された構成である。請求項３は、連結装置が両ピストンの後端から室外に延びる連結部を室外で連結した要素が請求項１又は請求項２に限定的に付加された構成である。請求項４は、貯留室に装着された第一ピストンとフィルターの間の空間に混合検体を入れるための開口が設けられ、且つ第一ピストン移動時に前記開口から外気が流入し又は室内の空気が外気中に流出することを阻止するための手段を有する要素が請求項１乃至請求項３のいずれかに限定的に付加された構成である。 A second aspect of the present invention is a configuration in which an element including means for preventing the liquid component entering the collection chamber from flowing back and out of the collection chamber is limited to the first aspect. According to a third aspect of the present invention, an element in which the coupling device is coupled outside the outdoor from the rear ends of the two pistons is added to the first or second aspect in a limited manner. According to a fourth aspect of the present invention, an opening is provided in the space between the first piston mounted in the storage chamber and the filter, and outside air flows in from the opening when the first piston moves, or indoor air flows An element having a means for preventing the air from flowing out into the outside air is a configuration that is limitedly added to any one of claims 1 to 3.

請求項５は、貯留室がシリンダーで構成され、そのシリンダーを分割して分離構造から取り外し再結合可能である要素が請求項１乃至請求項３のいずれかに限定的に付加された構成である。請求項６は、両ピストンの断面積が同一である要素が請求項１乃至請求項５のいずれかに限定的に付加された構成である。

According to a fifth aspect of the present invention, the storage chamber is constituted by a cylinder, and an element that can be detached from the separation structure and recombined by dividing the cylinder is limitedly added to any one of the first to third aspects. . A sixth aspect of the present invention has a configuration in which elements having the same cross-sectional area of both pistons are limitedly added to any one of the first to fifth aspects.

請求項１は、貯留室と採取室はフィルターを介して連通しており、貯留室及び採取室にそれぞれピストンが装着され、両ピストンは同時に同方向へ移動するように連結装置によって連結されている。両ピストンが連結されていないときは、貯留室に入っている混合検体をフィルターを通して採取室に送るために、まず採取室内の第二ピストンを引いて減圧する。そのときにフィルターで採取室と連通している貯留室も減圧されて貯留室内の第一ピストンがフィルター方向へ移動する。そして、第一ピストンは移動しながら混合検体をフィルターから採取室へと押出して液状成分が分離される。すなわち、第一ピストンをフィルター方向へ移動させるために減圧状態にするのである。このように、両ピストンが連結されていないときは、貯留室及び採取室を減圧するので検体も減圧されてしまうのである。これに対して請求項１は、前述したように両ピストンが連結されて同時に同方向へ移動する。したがって、採取室の第二ピストンを引いたときにそれと連結されている第一ピストンと同時に移動するので、第一ピストンを移動させるための減圧が不要である。また、第二ピストンと引いたときにその引いた容積分の空気が貯留室から送られて来るが、貯留室内の第一ピストンが移動して貯留室の容積を減じるので、貯留室及び採取室のいずれの室内でもほとんど減圧されないという効果を奏する。また、第二ピストンと引くと同時に第一ピストンが強制的にフィルター方向へ移動させられるので、液状成分の分離を迅速に行うことができる。   In the first aspect, the storage chamber and the collection chamber are communicated with each other through a filter, and a piston is attached to each of the storage chamber and the collection chamber, and both pistons are connected by a connecting device so as to move in the same direction at the same time. . When the two pistons are not connected, in order to send the mixed sample contained in the storage chamber to the collection chamber through the filter, the pressure is first reduced by pulling the second piston in the collection chamber. At that time, the storage chamber communicating with the collection chamber by the filter is also decompressed, and the first piston in the storage chamber moves in the filter direction. Then, while the first piston moves, the mixed specimen is extruded from the filter to the collection chamber, and the liquid component is separated. That is, in order to move the first piston in the filter direction, the pressure is reduced. Thus, when both pistons are not connected, the storage chamber and the sampling chamber are depressurized, so the specimen is also depressurized. On the other hand, according to the first aspect, as described above, both pistons are connected and simultaneously move in the same direction. Accordingly, when the second piston of the sampling chamber is pulled, the first piston connected to the second piston moves at the same time, so that no pressure reduction is required for moving the first piston. In addition, when the second piston is pulled, air corresponding to the drawn volume is sent from the storage chamber. However, since the first piston in the storage chamber moves to reduce the volume of the storage chamber, the storage chamber and the sampling chamber There is an effect that almost no pressure is reduced in any of the chambers. Further, since the first piston is forcibly moved in the filter direction at the same time as the second piston is pulled, the liquid component can be quickly separated.

請求項２は、採取室に入った液状成分が逆流して採取室から出ることを防止するための手段を備えており、そのままの状態で保存することができるから、液状成分をすぐに取り出す必要がなく取り扱いに便利である。請求項３は、連結装置は両ピストンの後端から室外に延びる連結部を室外で連結した構成である。したがって、室外の連結部を操作することによって第二ピストンを引くことができるから操作を容易に行うことができる。 Claim 2 is provided with means for preventing the liquid component entering the collection chamber from flowing back and exiting the collection chamber, and can be stored as it is, so it is necessary to immediately remove the liquid component. It is convenient for handling. According to a third aspect of the present invention, the connecting device has a structure in which connecting portions extending from the rear ends of both pistons to the outside are connected outdoors. Therefore, since the second piston can be pulled by operating the outdoor connecting portion, the operation can be easily performed.

請求項４は、貯留室に装着された第一ピストンとフィルターの間の空間に混合検体を入れるための開口が設けられ、且つ第一ピストン移動時に前記開口から外気が流入し又は室内の空気が外気中に流出することを阻止するための手段を有する構成である。したがって、混合検体を開口から貯留室に入れ、空気の出入りを阻止することにより、簡単に液状成分の分離操作をすることができる。 According to a fourth aspect of the present invention, an opening is provided in the space between the first piston mounted in the storage chamber and the filter, and outside air flows in from the opening when the first piston moves, or indoor air flows It is the structure which has a means for preventing outflow in outside air. Therefore, the liquid component can be easily separated by putting the mixed specimen into the storage chamber from the opening and preventing the air from entering and exiting.

請求項５は、貯留室がシリンダーで構成され、そのシリンダーを分割して分離構造から取り外し再結合可能である構成である。したがって、請求項４のように開口を設けなくても、貯留室を構成するシリンダーを外して貯留室を開き、その開いた口から混合検体を入れることができる。混合検体を入れた後に再びシリンダーを取付けることにより、簡単に液状成分の分離操作をすることができる。 According to a fifth aspect of the present invention, the storage chamber is constituted by a cylinder, and the cylinder can be divided and detached from the separation structure and recombined. Therefore, even if the opening is not provided as in the fourth aspect, the cylinder constituting the storage chamber can be removed to open the storage chamber, and the mixed specimen can be put through the opened port. The liquid component can be easily separated by attaching the cylinder again after putting the mixed specimen.

請求項６は、両ピストンの断面積が同一である構成である。したがって、両ピストンは同方向へ同時に移動するように連結されているから、同断面積の両ピストンが同じ距離だけ移動することになり、第二ピストンによる容積の増加と、第一ピストンによる容積の減少が同一である。これにより、第二ピストンを引いたときに同時に第一ピストンが同じ距離を移動するので減圧を生じないという効果を奏する。 A sixth aspect of the present invention has a configuration in which the cross-sectional areas of both pistons are the same. Therefore, since both pistons are connected so as to move simultaneously in the same direction, both pistons having the same cross-sectional area move by the same distance, and the volume increase by the second piston and the volume decrease by the first piston. Are the same. Thereby, since the 1st piston moves the same distance simultaneously when pulling a 2nd piston, there exists an effect that pressure reduction does not arise.

次に本発明を実施するための最良の形態について説明する。実施形態は、血液を希釈液で希釈した希釈血液から血漿を採取する分離構造に関する。図１に示すように貯留室１は貯留シリンダー２の中に形成され、図２に示すように採取室３は採取シリンダー４の中に形成される。両シリンダー２，４は一直線に連結されて連通しており、その境にフィルター５と逆止め弁６が介在している。貯留シリンダー２内に第一ピストン７が移動可能に装着されており、希釈血液を貯留室１内に入れるための開口８を塞がないように貯留シリンダー２の端部寄りに装着されている。第一ピストン７とフィルター５との間の空間が貯留室１であって、第一ピストン７の移動により広げたり狭くしたりすることができる。   Next, the best mode for carrying out the present invention will be described. The embodiment relates to a separation structure for collecting plasma from diluted blood obtained by diluting blood with a diluent. As shown in FIG. 1, the storage chamber 1 is formed in the storage cylinder 2, and the collection chamber 3 is formed in the collection cylinder 4 as shown in FIG. 2. Both cylinders 2 and 4 are connected in a straight line and communicate with each other, and a filter 5 and a check valve 6 are interposed at the boundary. A first piston 7 is movably mounted in the storage cylinder 2 and is mounted near the end of the storage cylinder 2 so as not to block the opening 8 for introducing diluted blood into the storage chamber 1. The space between the first piston 7 and the filter 5 is the storage chamber 1 and can be expanded or narrowed by the movement of the first piston 7.

採取シリンダー４の中に第二ピストン９が装着されており、使用前にこの第二ピストン９の先端は逆止め弁６と密接している。したがって、当初、採取室３は存在せず、第二ピストン９を引くことによって採取室３が形成される。しかし本発明の採取室３はこのような構成に限定されるものでなく、当初から第二ピストン９をやや引いた状態にして、逆止め弁６と第二ピストン９との間に隙間を設けて当初から採取室３を形成しておくこともできる。   A second piston 9 is mounted in the sampling cylinder 4 and the tip of the second piston 9 is in close contact with the check valve 6 before use. Therefore, initially, the collection chamber 3 does not exist, and the collection chamber 3 is formed by pulling the second piston 9. However, the collection chamber 3 of the present invention is not limited to such a configuration, and the second piston 9 is slightly pulled from the beginning, and a gap is provided between the check valve 6 and the second piston 9. Thus, the collection chamber 3 can be formed from the beginning.

第一ピストン７の後端から第一連結部１７が貯留シリンダー２の外部に延び、Ｕ字形に曲がって採取シリンダー４の端部まで届いている。また、第二ピストン９の後端から第二連結部１８が採取シリンダー４の外部に延び第一連結部１７とピン１９により結合している。したがって、第一ピストン７と第二ピストン９は連結部１７，１８により同時に同方向へ移動する。また、第一ピストン７と第二ピストン９の径は同一に形成されている。   A first connecting portion 17 extends from the rear end of the first piston 7 to the outside of the storage cylinder 2, bends in a U shape, and reaches the end of the sampling cylinder 4. A second connecting portion 18 extends from the rear end of the second piston 9 to the outside of the sampling cylinder 4 and is connected to the first connecting portion 17 by a pin 19. Accordingly, the first piston 7 and the second piston 9 are simultaneously moved in the same direction by the connecting portions 17 and 18. Moreover, the diameter of the 1st piston 7 and the 2nd piston 9 is formed identically.

フィルター５の採取シリンダー４側に逆止め弁６が装着され、フィルター５で濾過された血漿が採取シリンダー４内に送られ逆流を阻止することができる。図３は逆止め弁６を拡大した正面図であり、ゴムやエラストマーなどの柔軟性のある材料で円板状に形成され、３つの透孔１０が設けられている。周辺部１１は固定されており周辺部１１よりも内側の部分が撓むことになる。図３の紙面の裏側に通路１２が存在しており、この通路１２は透孔１０よりも内側にあって透孔１０と通路１２が重なることはない。この状態で貯留室１に希釈血液を入れ、開口８を栓３９で塞ぎ、第二ピストン９を紙面の手前側に引くと第一ピストン７も同時に移動して希釈血液を押し、フィルター５で濾過された血漿を採取室３に送り出す。この際、第一ピストン７を移動させるについて減圧する手段を用いず、連結部１７によって強制的に移動させるので検体をほとんど減圧させることがない。逆止め弁６が撓むことによって血漿が採取室３に送り出されるので、逆止め弁６が撓むことによる反力で検体が加圧されるがほとんど無視できる程度である。採取室３内に採取された血漿を取り出す方法としては種々あるが、例えばピン１９を抜き取り、採取シリンダー４の後端に螺合している蓋２５を取り外すことによって血漿を取り出すことができる。 A check valve 6 is attached to the collection cylinder 4 side of the filter 5, and the plasma filtered by the filter 5 can be sent into the collection cylinder 4 to prevent backflow. FIG. 3 is an enlarged front view of the check valve 6, which is formed in a disk shape from a flexible material such as rubber or elastomer, and is provided with three through holes 10. The peripheral portion 11 is fixed, and the inner portion of the peripheral portion 11 is bent. A passage 12 exists on the back side of the paper surface of FIG. 3, and this passage 12 is inside the through-hole 10 so that the through-hole 10 and the passage 12 do not overlap. In this state, diluted blood is put into the storage chamber 1, the opening 8 is closed with a stopper 39, and when the second piston 9 is pulled to the near side of the paper surface, the first piston 7 also moves simultaneously to push the diluted blood, and is filtered through the filter 5. The resulting plasma is sent out to the collection chamber 3. At this time, since the first piston 7 is moved by using the connecting portion 17 without using a pressure reducing means, the specimen is hardly depressurized. Since the plasma is sent to the collection chamber 3 when the check valve 6 bends, the specimen is pressurized by the reaction force caused by the deflection of the check valve 6, but is almost negligible. There are various methods for taking out the plasma collected in the collection chamber 3. For example, the plasma can be taken out by removing the pin 19 and removing the lid 25 screwed into the rear end of the collection cylinder 4.

また、逆止め弁６が撓むことによる加圧を避けるために逆止め弁を用いず、図４に示すような逆止め機能を有するフィルター部材１３を用いることによって逆流を防止でき加圧も生じない。このフィルター部材１３を用いるときは図１に示すフィルター５が不要であることは勿論である。フィルター部材１３は細長い矩形に形成され、一方の端部寄りに孔１４が形成され内部にフィルター１５が設けられている。このフィルター部材１３を摺動可能に貯留室１と採取室３との間に装着し、当初は通路１２にフィルター１５を合わせておき、血漿を分離した後でフィルター部材１３を摺動してフィルター１５を通路１２から外し、シャッター部１６を通路１２に合わせることで通路１２を遮断し逆流を防止できる。この場合、逆止め弁がなく、第一ピストン７で押出した空気及び希釈血液の合計容積と、第二ピストン９の移動によって広がる採取室３の容積が同じであるために減圧状態も加圧状態も生じることがない。 Further, by using a filter member 13 having a check function as shown in FIG. 4 without using a check valve in order to avoid pressurization due to bending of the check valve 6, backflow can be prevented and pressurization also occurs. Absent. Of course, when the filter member 13 is used, the filter 5 shown in FIG. 1 is unnecessary. The filter member 13 is formed in an elongated rectangular shape, a hole 14 is formed near one end, and a filter 15 is provided inside. The filter member 13 is slidably mounted between the storage chamber 1 and the collection chamber 3, and initially the filter 15 is fitted to the passage 12, and after separating the plasma, the filter member 13 is slid to filter By removing 15 from the passage 12 and aligning the shutter portion 16 with the passage 12, the passage 12 can be blocked to prevent backflow. In this case, since there is no check valve, the total volume of the air and diluted blood pushed out by the first piston 7 and the volume of the collection chamber 3 that is expanded by the movement of the second piston 9 are the same, so that the decompressed state is also pressurized. Will not occur.

さらに、図４に示すフィルター部材１３に代えて図５に示す逆止め機能を有するフィルター部材２０を用いることもできる。このフィルター部材２０を用いるときも図１に示すフィルター５は不要である。フィルター部材２０は円筒体２１の胴部に孔２２を形成し、その中にフィルター２３が取付けられている。また、端部にレバー２４が設けられている。フィルター部材２０を回動可能に貯留室１と採取室３との間に装着し、当初は通路１２にフィルター２３を合わせておき、血漿を分離した後でレバー２４を摘んでフィルター部材２０を９０度回動してフィルター２３を通路１２から外し、円筒体２１の表面を通路１２に合わせることで通路１２を遮断し逆流を防止できる。この場合も逆止め弁がなく、第一ピストン７で押出した空気及び希釈血液の合計容積と、第二ピストン９の移動によって広がる採取室３の容積が同じであるために減圧状態も加圧状態も生じることがない。 Furthermore, it can replace with the filter member 13 shown in FIG. 4, and the filter member 20 which has a non-return function shown in FIG. 5 can also be used. Also when this filter member 20 is used, the filter 5 shown in FIG. 1 is unnecessary. The filter member 20 has a hole 22 formed in the body portion of the cylindrical body 21, and a filter 23 is mounted therein. A lever 24 is provided at the end. The filter member 20 is rotatably mounted between the storage chamber 1 and the collection chamber 3, and the filter 23 is initially aligned with the passage 12, and after the plasma is separated, the lever 24 is picked to move the filter member 20 to 90. The filter 23 is turned off to remove the filter 23 from the passage 12, and the surface of the cylindrical body 21 is aligned with the passage 12, thereby blocking the passage 12 and preventing backflow. Also in this case, since there is no check valve, the total volume of the air and diluted blood pushed out by the first piston 7 and the volume of the collection chamber 3 that is expanded by the movement of the second piston 9 are the same, so that the decompressed state is also the pressurized state. Will not occur.

図６は、開口８を設けないで他の方法で希釈血液を貯留室１に入れる構造を示したものである。この構造は、貯留シリンダー２がネジ２６で分割可能であり、分割分離された分割シリンダー２７を下にして、この分割シリンダー２７の中に希釈血液を入れて再びネジ２６で貯留シリンダー２を１つに結合することによって希釈血液を貯留室１に入れることができる。   FIG. 6 shows a structure in which diluted blood is put into the storage chamber 1 by another method without providing the opening 8. In this structure, the storage cylinder 2 can be divided by a screw 26, the divided cylinder 27 is divided and separated, the diluted blood is put into the divided cylinder 27, and one storage cylinder 2 is again formed by the screw 26. The diluted blood can be put into the storage chamber 1 by binding to.

図７は、血液の希釈容器４０から希釈血液を貯留室１に入れる構成を示したものである。図７は希釈容器４０と本発明の分離構造が結合した状態を示しているが、希釈容器４０と分離構造は当初は結合されておらず、希釈容器４０に血液を採取して希釈液で希釈した後に分離構造に結合されるものである。希釈容器４０は、容器本体２８と希釈液の封入体を兼ねる蓋２９とから成り、容器本体２８の底部に孔３０が設けられ閉塞部３５で塞がれている。容器本体２８の上半部にはそれよりも面積の小さなプラスチック製受け皿３２が取り付けられている。さらに細長い上向きのカッター３３が受け皿３２と一体に形成されている。   FIG. 7 shows a configuration in which diluted blood is put into the storage chamber 1 from the blood dilution container 40. FIG. 7 shows a state in which the dilution container 40 and the separation structure of the present invention are combined. However, the dilution container 40 and the separation structure are not initially connected, and blood is collected in the dilution container 40 and diluted with the diluent. After that, it is bonded to the separation structure. The dilution container 40 includes a container main body 28 and a lid 29 that also serves as an enclosure for the diluent. A hole 30 is provided at the bottom of the container main body 28 and is closed by a closing portion 35. A plastic tray 32 having a smaller area is attached to the upper half of the container body 28. Further, an elongated upward cutter 33 is formed integrally with the receiving tray 32.

蓋２９の下面に希釈液の封入室３８が設けられ防水シート３１で希釈液が封入されている。蓋２９を回動して容器本体２８への螺合作業を開始すると蓋２９が下降してカッター３３が防水シート３１に突き刺さり、さらに蓋２９を回動させることによって防水シート３１を破くことができる。血液を希釈して貯留室１に入れるためには、まず別体の希釈容器４０を用意し、ランセットを用いて指等に針等を刺して得られた血液を受け皿３２の上に落とし、次いで容器本体２８の上に蓋２９を被せて回動しながら防水シート３１を破いて希釈液を受け皿３２の上に流し、希釈された血液を容器本体２８に流す。蓋２９が容器本体２８に完全に結合したときに希釈容器を逆さにするなどして血液と希釈液を混合する。混合が終了したときに図７に示すように希釈容器４０を分離構造に嵌合又は螺合により取り付ける。この取付けの際に、カッター３４が閉塞部３５を破り、希釈された血液が通路３６及び流入口３７を通って貯留室１に流れ込む。なお、本発明において希釈血液を貯留室１に入れる構造あるいは他の構造について以上述べた実施形態に限定されないことは勿論である。   A diluting liquid sealing chamber 38 is provided on the lower surface of the lid 29, and the diluting liquid is sealed with a waterproof sheet 31. When the lid 29 is rotated and the screwing operation to the container body 28 is started, the lid 29 is lowered, the cutter 33 pierces the waterproof sheet 31, and the cover 29 is further rotated to break the waterproof sheet 31. . In order to dilute the blood into the storage chamber 1, first, a separate dilution container 40 is prepared, and the blood obtained by inserting a needle into a finger or the like using a lancet is dropped on the receiving tray 32, and then The cover 29 is put on the container main body 28, and the waterproof sheet 31 is broken while rotating to flow the diluted liquid on the receiving tray 32, and the diluted blood is flowed to the container main body 28. When the lid 29 is completely connected to the container body 28, the blood and the diluent are mixed, for example, by inverting the dilution container. When mixing is completed, the dilution container 40 is attached to the separation structure by fitting or screwing as shown in FIG. During this attachment, the cutter 34 breaks the blocking portion 35, and the diluted blood flows into the storage chamber 1 through the passage 36 and the inlet 37. Of course, the present invention is not limited to the embodiment described above with respect to the structure in which the diluted blood is placed in the storage chamber 1 or other structures.

本発明の希釈血液分離前の断面図Cross-sectional view before separation of diluted blood of the present invention 本発明の液状成分分離後の断面図Sectional view after separation of liquid component of the present invention 逆止め弁の正面図Front view of check valve 逆止め機能を有するフィルター部材の斜視図Perspective view of filter member having non-return function 逆止め機能を有する他のフィルター部材の斜視図Perspective view of another filter member having a check function 本発明の他の実施形態の断面図Sectional drawing of other embodiment of this invention 希釈容器を結合した本発明の断面図Cross-sectional view of the present invention with a dilution vessel coupled

符号の説明Explanation of symbols

１ 貯留室
２ 貯留シリンダー
３ 採取室
４ 採取シリンダー
５ フィルター
６ 逆止め弁
７ 第一ピストン
８ 開口
９ 第二ピストン
１０ 透孔
１１ 周辺部
１２ 通路
１３ フィルター部材
１４ 孔
１５ フィルター
１６ シャッター部
１７ 第一連結部
１８ 第二連結部
１９ ピン
２０ フィルター部材
２１ 円筒体
２２ 孔
２３ フィルター
２４ レバー
２５ 蓋
２６ ネジ
２７ 分割シリンダー
２８ 容器本体
２９ 蓋
３０ 孔
３１ 防水シート
３２ 受け皿
３３ カッター
３４ カッター
３５ 閉塞シート
３６ 通路
３７ 流入口
３８ 封入室
３９ 栓
４０ 希釈容器 DESCRIPTION OF SYMBOLS 1 Storage chamber 2 Storage cylinder 3 Sampling chamber 4 Sampling cylinder 5 Filter 6 Check valve 7 1st piston 8 Opening 9 2nd piston 10 Through-hole 11 Peripheral part 12 Passage 13 Filter member 14 Hole 15 Filter 16 Shutter part 17 First connection Portion 18 second connecting portion 19 pin 20 filter member 21 cylindrical body 22 hole 23 filter 24 lever 25 lid 26 screw 27 split cylinder 28 container body 29 lid 30 hole 31 waterproof sheet 32 tray 33 cutter 34 cutter 35 closing sheet 36 passage 37 flow Inlet 38 Enclosure chamber 39 Plug 40 Dilution container

Claims (6)

検体と加え液を混合した混合検体を入れるための貯留室と、その混合検体から分離した液状成分を入れるための採取室を有し、貯留室と採取室はフィルターを介して連通しており、貯留室及び採取室にそれぞれピストンが装着され、両ピストンは同時に同方向へ移動するように連結装置によって連結されていることを特徴とする分離構造 It has a storage chamber for storing a mixed sample in which a sample and an added liquid are mixed, and a collection chamber for storing a liquid component separated from the mixed sample, and the storage chamber and the collection chamber communicate with each other through a filter. A separation structure characterized in that a piston is attached to each of the storage chamber and the sampling chamber, and both pistons are connected by a connecting device so as to move in the same direction at the same time. 採取室に入った液状成分が逆流して採取室から出ることを防止するための手段を備えている請求項１記載の分離構造 2. The separation structure according to claim 1, further comprising means for preventing the liquid component entering the collection chamber from flowing back and exiting the collection chamber. 連結装置は両ピストンの後端から室外に延びる連結部を室外で連結したものである請求項１又は請求項２記載の分離構造 The separation structure according to claim 1 or 2, wherein the coupling device is configured by coupling a coupling portion extending from the rear ends of both pistons to the outside of the room. 貯留室に装着された第一ピストンとフィルターの間の空間に混合検体を入れるための開口が設けられ、且つ第一ピストン移動時に前記開口から外気が流入し又は室内の空気が外気中に流出することを阻止するための手段を有する請求項１乃至請求項３のいずれかに記載の分離構造 An opening is provided in the space between the first piston mounted in the storage chamber and the filter, and outside air flows in from the opening or indoor air flows out into the outside air when the first piston moves. A separation structure according to any one of claims 1 to 3 , further comprising means for preventing this. 貯留室はシリンダーで構成され、そのシリンダーを分割して分離構造から取り外し再結合可能である請求項１乃至請求項３のいずれかに記載の分離構造 The separation structure according to any one of claims 1 to 3 , wherein the storage chamber is composed of a cylinder, and the cylinder can be divided and detached from the separation structure and recombined. 両ピストンの断面積は同一である請求項１乃至請求項５のいずれかに記載の分離構造 The separation structure according to any one of claims 1 to 5 , wherein both pistons have the same cross-sectional area.
JP2004292421A 2004-10-05 2004-10-05 Separation structure for separating liquid components from a mixed sample in which the sample and additive liquid are mixed Expired - Fee Related JP4028865B2 (en)

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JP7023124B2 (en) 2018-01-26 2022-02-21 セイコーインスツル株式会社 Movement and watches

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Publication number Priority date Publication date Assignee Title
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