JP3981105B2 - Separation device for mixing sample and added liquid to separate liquid components - Google Patents

Separation device for mixing sample and added liquid to separate liquid components Download PDF

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JP3981105B2
JP3981105B2 JP2004226137A JP2004226137A JP3981105B2 JP 3981105 B2 JP3981105 B2 JP 3981105B2 JP 2004226137 A JP2004226137 A JP 2004226137A JP 2004226137 A JP2004226137 A JP 2004226137A JP 3981105 B2 JP3981105 B2 JP 3981105B2
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眞康 須田
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株式会社ヒポクラテス
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Description

本発明は比較的微量の検体に加え液を加えて混合し、液状成分を分離するための分離装置に係り、主に血液から血漿を分離するために用いられる。   The present invention relates to a separation apparatus for separating a liquid component by adding a liquid to a relatively small amount of sample and mixing the liquid, and is mainly used for separating plasma from blood.

従来の血液成分分離装置は、血液成分と化学物質を含む溶液とを混合するための手段と、血液成分から主に血漿成分を分離するための分離手段を有し、且つ前記混合するための手段と前記分離手段とを接続するための連結手段を有し、さらに必要に応じて加圧または引圧を生じさせる駆動手段を有する装置が存在している（例えば、特許文献１参照。）。
特開２００３−１４９２３４号公報 A conventional blood component separation apparatus has means for mixing a blood component and a solution containing a chemical substance, and means for separating mainly a plasma component from the blood component, and means for mixing There is a device having a connecting means for connecting the separator and the separating means, and further having a driving means for generating pressurization or pulling pressure as required (see, for example, Patent Document 1).
JP 2003-149234 A

本発明は、血液などの検体と加え液を混合し液状成分を分離するための分離装置であって、製造工程を短くしてコストを抑え、且つ検体の成分分離を容易に行うことを課題とする。前記特許文献１の分離装置は、化学物質溶解液の入った混和容器の中に血液を添加し、その化学物質溶解液と血液の混和液中の血漿成分のみを分離手段で分離して透過させ、その血漿成分を採取容器に分離採取する構成である。そして、混和容器は、混和液を分離手段に送るための開口部が設けられており、当初はその開口部が閉塞されて混和容器の内部と分離手段は遮断され、化学物質溶解液と血液を混和してその混和液を分離手段に送るときにその開口部を開くための手段が設けられている。   The present invention is a separation apparatus for separating a liquid component by mixing a sample such as blood with a liquid to be added, and it is an object to shorten the manufacturing process to reduce the cost and to easily separate the component of the sample. To do. In the separation device of Patent Document 1, blood is added to a mixing container containing a chemical substance solution, and only the plasma component in the chemical substance solution and the blood mixture is separated and permeated by a separation means. The plasma component is separated and collected in a collection container. The mixing container is provided with an opening for sending the mixed solution to the separation means. Initially, the opening is closed, the inside of the mixing container and the separation means are shut off, and the chemical substance solution and blood are removed. Means are provided for opening the opening when mixing and sending the mixture to the separating means.

従来の分離装置は上述したような構成であって、混和容器の開口部は当初閉塞されていて、混和が完了して混和液を分離手段に送るときにその開口部を開くことが必要である。閉塞された開口部を開くときは、閉塞部材がシールであるときはそれを破る装置が必要であり、閉塞部材がキャップであるときはそれを手で取り外すことが必要であり、閉塞部材が活栓であるときはそれを開かなければならない。したがって、分離装置の製造について閉塞部材を取り付ける工程と、その閉塞部材を開く工程が必要であり、製造工程が複雑になりコストが嵩むことになる。そこで本発明は、血液成分分離工程において分離手段の前にシールなどの閉塞部材を設けることの必要がない分離装置を提供することを目的とする。   The conventional separation apparatus is configured as described above, and the opening of the mixing container is initially closed, and it is necessary to open the opening when mixing is completed and the mixture is sent to the separation means. . When opening a closed opening, a device that breaks it when the closing member is a seal is required, and when the closing member is a cap, it is necessary to remove it manually, and the closing member is a stopcock. When it is, you must open it. Therefore, a step of attaching the closing member and a step of opening the closing member are necessary for the manufacture of the separation device, which complicates the manufacturing process and increases the cost. Accordingly, an object of the present invention is to provide a separation device that does not require a closing member such as a seal before the separation means in the blood component separation step.

さらに特許文献１に示された従来の分離装置は、混和容器にキャップを取り外す作業、混和容器と分離装置を結合するための作業、プランジャーを押したり引いたりする作業、活栓を動かす作業などの異なる形態の作業が組合わされている。したがって、作業の順番ごとに異なる作業を行わなければならず、分離作業全体が単純でないために取り扱いにくく、場合によっては作業やその順番を誤る虞がある。そこで、本発明はプランジャーを押すか引くかの単純な作業のみで分離作業のできる分離装置を提供することを目的とする。   Furthermore, the conventional separation apparatus disclosed in Patent Document 1 includes a work for removing a cap from a mixing container, a work for connecting the mixing container and the separation apparatus, a work for pushing and pulling a plunger, and a work for moving a stopcock. Different forms of work are combined. Therefore, a different work must be performed for each work order, and the whole separation work is not simple, so it is difficult to handle. In some cases, there is a possibility that the work and its order may be wrong. SUMMARY OF THE INVENTION An object of the present invention is to provide a separation apparatus that can perform a separation operation only by a simple operation of pushing or pulling a plunger.

本発明は、検体と加え液を混合する混合構造と、検体と加え液を混合した混合検体から液状成分を分離するための分離構造を含み、前記混合構造は検体と加え液を混合するための混合室を有し、前記分離構造は貯留室と採取室を有し、貯留室は混合室と連通し、採取室はフィルターを介して貯留室と連通し、且つ、混合室内の混合検体を貯留室に送るための手段と、貯留室に送られた混合検体を前記フィルターに通し液状成分を分離して採取室に送る手段とを備えており、前記混合構造は、加え液を蓄えた液封入シリンダーを有し、この液封入シリンダーの先端に設けられた開口部により液封入シリンダーと混合室が連通し、且つ前記開口部は閉塞部材で閉塞され、液封入シリンダー内プランジャーと前記閉塞部材との間の空所に加え液が蓄えられており、前記液封入シリンダー内プランジャーを押すことによって閉塞部材が開通し、加え液が混合室に送られ、前記混合室の内壁はシリンダー壁状に形成され、液封入シリンダーが混合室内を擦動できるように装着されており、液封入シリンダーを押すことによって混合検体が混合室から前記貯留室に送られ、液封入シリンダー内プランジャーの後端は液封入シリンダーの後端よりも後方に突き出ており、
貯留室の内壁はシリンダー壁状に形成されその貯留室内にピストンが装着されており、混合検体は混合室から前記ピストンとフィルターの間の部分に送られ、混合検体の圧力によってピストンはフィルターと反対方向に擦動して混合検体は貯留室内に入り、採取室の内壁はシリンダー壁状に形成され採取プランジャーが装着されており、採取プランジャーを引くことによりフィルターを介して貯留室内の液状成分を採取室内に送る構成である。 The present invention includes a mixing structure for mixing a specimen and an addition liquid, and a separation structure for separating a liquid component from a mixed specimen obtained by mixing the specimen and the addition liquid, the mixing structure for mixing the specimen and the addition liquid. The separation structure has a storage chamber and a collection chamber, the storage chamber communicates with the mixing chamber, the collection chamber communicates with the storage chamber via a filter, and stores the mixed specimen in the mixing chamber Means for sending to the chamber, and means for passing the mixed specimen sent to the storage chamber through the filter to separate the liquid component and sending it to the collection chamber. The liquid-filled cylinder and the mixing chamber communicate with each other through an opening provided at the tip of the liquid-filled cylinder, and the opening is closed with a closing member. In addition to the void between The closing member is opened by pushing the plunger in the liquid-sealed cylinder, the added liquid is sent to the mixing chamber, the inner wall of the mixing chamber is formed in the shape of a cylinder wall, and the liquid-sealed cylinder passes through the mixing chamber. It is mounted so that it can be rubbed, and the mixed specimen is sent from the mixing chamber to the storage chamber by pushing the liquid sealing cylinder, and the rear end of the plunger in the liquid sealing cylinder is behind the rear end of the liquid sealing cylinder. Stick out,
The inner wall of the storage chamber is formed in the shape of a cylinder wall, and a piston is mounted in the storage chamber. The mixed sample is sent from the mixing chamber to the portion between the piston and the filter, and the piston is opposed to the filter by the pressure of the mixed sample. The mixed specimen enters the storage chamber by rubbing in the direction, the inner wall of the collection chamber is formed in the shape of a cylinder wall, and a collection plunger is attached. By pulling the collection plunger, the liquid component in the storage chamber is passed through the filter. Is sent to the collection chamber.

混合構造が検体と加え液を混合するための混合室を有し、分離構造は貯留室と採取室を有し、貯留室は混合室と連通し、採取室はフィルターを介して貯留室と連通し、且つ、混合室内の混合検体を貯留室に送るための手段と、貯留室に送られた混合検体混合検体を前記フィルターに通し液状成分を分離して採取室に送る手段とを備えている。したがって、混合室とフィルターは隣接しておらず、それらの間に貯留室が介在している。したがって、検体を混合室内で混合するときに混合検体は直接フィルターに接しないから、混合検体がフィルターに接することを防止するための遮断装置を必要とせず、その遮断装置を開放するための装置も必要としないという効果を奏する。   The mixing structure has a mixing chamber for mixing the sample and the added liquid, the separation structure has a storage chamber and a sampling chamber, the storage chamber communicates with the mixing chamber, and the sampling chamber communicates with the storage chamber via a filter. And a means for sending the mixed specimen in the mixing chamber to the storage chamber and a means for passing the mixed specimen mixed specimen sent to the storage chamber through the filter to separate the liquid component and sending it to the collection chamber . Therefore, the mixing chamber and the filter are not adjacent to each other, and the storage chamber is interposed between them. Therefore, when the sample is mixed in the mixing chamber, the mixed sample does not directly contact the filter, so a blocking device for preventing the mixed sample from contacting the filter is not required, and a device for opening the blocking device is also available. There is an effect that it is not necessary.

また、混合構造は、加え液を蓄えた液封入シリンダーを有し、この液封入シリンダーの先端に設けられた開口部により液封入シリンダーと混合室が連通し、且つ前記開口部は閉塞部材で閉塞され、液封入シリンダー内プランジャーと前記閉塞部材との間の空所に加え液が蓄えられており、前記液封入シリンダー内プランジャーを押すことによって閉塞部材が開通し、加え液が混合室に送られる。血液などの検体と加え液とを混合するときに、最初に血液などの検体を入れてそこに加え液を入れると、加え液の流れる勢いで血液などの検体が加え液と攪拌するように混合されるので、混合を迅速に行うことができる。これに対して従来の分離装置は、混合室にあらかじめ加え液が入れてあり、そこに血液を添加して混合する構成である。加え液に対して血液は微量であるから、血液を添加しても攪拌のような状態は生じないから、手で十分に振らなくてはならず混合を迅速に行うことができないのである。   Further, the mixing structure has a liquid-filled cylinder that stores the added liquid, the liquid-filled cylinder and the mixing chamber communicate with each other through an opening provided at the tip of the liquid-filled cylinder, and the opening is closed by a closing member. In addition, liquid is stored in a space between the plunger in the liquid-filled cylinder and the closing member, and the closing member is opened by pushing the plunger in the liquid-filled cylinder, so that the added liquid enters the mixing chamber. Sent. When mixing a sample such as blood with an added solution, first add the sample such as blood and then add the added solution to the sample. Therefore, mixing can be performed quickly. On the other hand, the conventional separation device has a configuration in which a liquid is previously added to a mixing chamber, and blood is added to the mixing chamber for mixing. Since the amount of blood relative to the added solution is very small, even if blood is added, a state such as stirring does not occur.

また、混合室の内壁がシリンダー壁状に形成され、液封入シリンダーが混合室内を擦動できるように装着されており、液封入シリンダーを押すことによって混合検体が混合室から前記貯留室に送られる。フィルターは貯留室と採取室の間に介在していて、フィルターと混合室の間には貯留室があるので、混合室内では検体と加え液をフィルターに接することなく混合することができる。したがって、混合時に混合検体とフィルターを遮断するための手段を必要としないのでその分簡単な構造とすることができる。また、混合検体の分離を行うために混合室から貯留室に送る作業はプランジャーを一回押すだけであるから、作業が単純で簡単に行うことができる。さらに、混合検体が貯留室に送られたときはフィルターと接するが、すでに混合室で十分に混合されているのでフィルターに吸い取られても診断等に不都合は生じない。従来の分離装置は、容器内で混合してすぐにフィルターを通す構造であったから、容器とフィルターの間を遮断しないと加え液のみが先にフィルターに吸い取られ、その後で血液を添加することになるから十分な混合ができず、遮断装置は必須であった。   In addition, the inner wall of the mixing chamber is formed into a cylinder wall shape, and the liquid-filled cylinder is mounted so that it can be rubbed in the mixing chamber. . Since the filter is interposed between the storage chamber and the collection chamber, and there is a storage chamber between the filter and the mixing chamber, the sample and the added liquid can be mixed without contacting the filter in the mixing chamber. Accordingly, since no means for blocking the mixed specimen and the filter at the time of mixing is required, the structure can be simplified accordingly. Moreover, since the operation | work sent from a mixing chamber to a storage chamber in order to isolate | separate a mixing specimen only pushes a plunger once, an operation | work can be performed simply and easily. Further, when the mixed specimen is sent to the storage chamber, it comes into contact with the filter, but since it is already sufficiently mixed in the mixing chamber, there is no inconvenience for diagnosis or the like even if it is sucked by the filter. Since the conventional separation device has a structure in which a filter is passed immediately after mixing in a container, unless the container and the filter are cut off, only the added liquid is sucked into the filter first, and then blood is added. Therefore, sufficient mixing was not possible, and a shut-off device was essential.

また、液封入シリンダー内プランジャーの後端は液封入シリンダーの後端よりも後方に突き出ている。したがって、液封入シリンダー内プランジャーを押すことにより加え液が混合室に入り、さらに押すことによって液封入シリンダーが前進して混合検体を貯留室に押し出すことができる。   Further, the rear end of the plunger in the liquid sealing cylinder protrudes rearward from the rear end of the liquid sealing cylinder. Therefore, the added liquid enters the mixing chamber by pushing the plunger in the liquid-filled cylinder, and further pushes the liquid-filled cylinder to push the mixed specimen into the storage chamber.

また、貯留室の内壁はシリンダー壁状に形成されその貯留室内にピストンが装着されており、混合検体は混合室から前記ピストンとフィルターの間の部分に送られ、混合検体の圧力によってピストンはフィルターと反対方向に擦動して混合検体は貯留室内に入ることができる。   Further, the inner wall of the storage chamber is formed in a cylinder wall shape, and a piston is mounted in the storage chamber, and the mixed specimen is sent from the mixing chamber to a portion between the piston and the filter, and the piston is filtered by the pressure of the mixed specimen. The mixed specimen can enter the storage chamber by rubbing in the opposite direction.

また、、採取室の内壁はシリンダー壁状に形成され採取プランジャーが装着されており、採取プランジャーを引くことによりフィルターを介して貯留室内の液状成分を採取室内に送る構成である。したがって、採取プランジャーを引くことによって液状成分を採取することができる。すなわち、分離作業は混合構造に設けられている二重構造のプランジャーを押し、分離構造に設けられている採取プランジャーを引くだけのプランジャーのみの単純な操作で行うことができるから、誰でも容易に間違いなく作業することができる。   Further, the inner wall of the collection chamber is formed in the shape of a cylinder wall and is equipped with a collection plunger. By pulling the collection plunger, the liquid component in the storage chamber is sent into the collection chamber through a filter. Therefore, the liquid component can be collected by pulling the collection plunger. In other words, the separation operation can be performed by a simple operation of only the plunger that pushes the plunger of the double structure provided in the mixing structure and pulls the sampling plunger provided in the separation structure. But you can easily work without doubt.

次に本発明を実施するための最良の形態について説明する。実施形態は、血液を加え液で希釈した後に血漿を採取する分離装置に関する。図１に示すように混合装置１の混合室２は混合シリンダー１５に設けられており、その混合シリンダー１５の側部に血液を取り入れるための口部１０が形成されている。混合室２の中に擦動可能な液封入シリンダー３が装着されていて、その液封入シリンダー３にはシール４が設けられているが、液封入シリンダー３と混合室２の機密性が保たれる構造であるときは、シール４は必ずしも必要ではない。液封入シリンダー３の先端は開放され開口部５が形成されており、その開口部５は逆止め弁６によって閉塞されている。   Next, the best mode for carrying out the present invention will be described. The embodiment relates to a separation apparatus for collecting plasma after adding blood and diluting with a liquid. As shown in FIG. 1, the mixing chamber 2 of the mixing device 1 is provided in a mixing cylinder 15, and a mouth portion 10 for taking blood is formed in a side portion of the mixing cylinder 15. A slidable liquid-filled cylinder 3 is mounted in the mixing chamber 2, and a seal 4 is provided on the liquid-filled cylinder 3, but the confidentiality of the liquid-filled cylinder 3 and the mixing chamber 2 is maintained. The seal 4 is not always necessary when the structure is such that The front end of the liquid sealing cylinder 3 is opened to form an opening 5, and the opening 5 is closed by a check valve 6.

液封入シリンダー３はシリンダー状に形成されており、その内部に液封入シリンダー内プランジャー７が装着されている。液封入シリンダー内プランジャー７にはシール８が設けられているが、液封入シリンダー内プランジャー７と液封入シリンダー３の内壁との機密性が保たれる構造であるときは、シール８は必ずしも必要ではない。前述した液封入シリンダー３の先端に取り付けられた逆止め弁６と液封入シリンダー内プランジャー７の先端との間に空所９ができるように液封入シリンダー内プランジャー７はやや後方に引いた状態で装着されており、その空所９内に加え液が蓄えられている。液封入シリンダー内プランジャー７を押すとその圧力で逆止め弁６が開き、加え液が混合室２に流れ込む。   The liquid-filled cylinder 3 is formed in a cylindrical shape, and a plunger 7 in the liquid-filled cylinder is mounted therein. The plunger 8 in the liquid-filled cylinder is provided with a seal 8. However, when the confidentiality between the plunger 7 in the liquid-filled cylinder and the inner wall of the liquid-filled cylinder 3 is maintained, the seal 8 is not necessarily provided. Not necessary. The plunger 7 in the liquid cylinder is pulled slightly rearward so that a space 9 is formed between the check valve 6 attached to the tip of the liquid cylinder 3 and the tip of the plunger 7 in the liquid cylinder. It is mounted in a state, and liquid is stored in the space 9. When the plunger 7 in the liquid sealing cylinder is pushed, the check valve 6 is opened by the pressure, and the added liquid flows into the mixing chamber 2.

混合室２の先端と貯留室１１の側部は通路１７で連通しており、この通路１７は混合室２よりも狭く形成され、通路１７の内径は混合室２の内径の１４％程度である。しかし、この数値に限定されるものではなく例えば１０％〜８０％であってもよい。したがって、６０％、５０％、４０％、３０％、２０％あるいは１２％程度であってもよい。   The front end of the mixing chamber 2 and the side of the storage chamber 11 communicate with each other through a passage 17. The passage 17 is formed narrower than the mixing chamber 2, and the inner diameter of the passage 17 is about 14% of the inner diameter of the mixing chamber 2. . However, it is not limited to this numerical value, and may be 10% to 80%, for example. Therefore, it may be about 60%, 50%, 40%, 30%, 20% or 12%.

分離装置１６の貯留室１１は貯留シリンダー１２の中に設けられ、採取室１３は採取シリンダー１４の中に設けられる。両シリンダー１２，１４は一直線に連結されて連通しており、その境にフィルター１９と逆止め弁２０が介在している。貯留シリンダー１４内にピストン１８が擦動可能に装着されており、通路１７を塞がないように通路１７のやや手前の位置に置かれている。ピストン１８とフィルター１９との間の空間が貯留室１１であって、ピストン１８の擦動により広げることができる。フィルター１９の採取シリンダー１４側に逆止め弁が装着され、フィルター１９で濾過された液状成分が採取シリンダー１４内に送られ逆流を阻止することができる。   The storage chamber 11 of the separation device 16 is provided in the storage cylinder 12, and the collection chamber 13 is provided in the collection cylinder 14. Both cylinders 12 and 14 are connected in a straight line and communicate with each other, and a filter 19 and a check valve 20 are interposed at the boundary. A piston 18 is slidably mounted in the storage cylinder 14 and is placed slightly in front of the passage 17 so as not to block the passage 17. A space between the piston 18 and the filter 19 is the storage chamber 11 and can be expanded by friction of the piston 18. A check valve is mounted on the collection cylinder 14 side of the filter 19, and the liquid component filtered by the filter 19 can be sent into the collection cylinder 14 to prevent backflow.

採取シリンダー１４の中に採取プランジャー２１が装着されており、この採取プランジャー２１の先端に取り付けられたシール２２は逆止め弁２０と密接している。したがって、当初、採取室１３は存在せず、採取プランジャー２１を引くことによって採取室１３が形成される。しかし本発明の採取室１３はこのような構成に限定されるものでなく、当初から採取プランジャー２１をやや引いた状態にして、逆止め弁２０とシール２２との間に隙間を設けて当初から採取室１３を形成しておくこともできる。また、前述したように両シリンダー１２，１４は一直線に連結されていて、混合シリンダー１５に対して垂直方向に延びている。しかし、本発明はこの構成に限定されるものではなく両シリンダー１２，１４と混合シリンダー１５を同じ方向に延びるように構成してもよい。この場合は、通路１７を短くするために混合シリンダー１７の先端をフィルター１９の側方に位置させるとよい。なお、前述した逆止め弁６，２０は図５に拡大して示した構造であって、ゴムやエラストマーなどの柔軟性のある材料で円板状に形成され、３つの透孔２３が設けられている。周辺部２４は固定されており紙面の手前側が減圧側であってプランジャーで引く側である。紙面の裏側に開口部２５が存在しており、この開口部２５は透孔２３よりも内側にあって透孔２３と開口部２５が重なることはない。この状態でプランジャーを紙面の手前側に引くと減圧されて逆止め弁６，２０も手前側に膨らむから、逆止め弁６，２０で閉塞されていた通路２５が開通し、逆止め弁６，２０の裏側を通り透孔２３と通って混合検体紙面の手前側に送られる。ただし、本発明の逆止め弁がこの構成に限定されないことは勿論である。   A sampling plunger 21 is mounted in the sampling cylinder 14, and a seal 22 attached to the tip of the sampling plunger 21 is in close contact with the check valve 20. Therefore, initially, the collection chamber 13 does not exist, and the collection chamber 13 is formed by pulling the collection plunger 21. However, the collection chamber 13 of the present invention is not limited to such a configuration, and the collection plunger 21 is slightly pulled from the beginning, and a gap is provided between the check valve 20 and the seal 22 at the beginning. The sampling chamber 13 can also be formed. Further, as described above, both the cylinders 12 and 14 are connected in a straight line and extend in a direction perpendicular to the mixing cylinder 15. However, the present invention is not limited to this configuration, and both the cylinders 12 and 14 and the mixing cylinder 15 may be configured to extend in the same direction. In this case, the tip of the mixing cylinder 17 may be positioned on the side of the filter 19 in order to shorten the passage 17. The above-described check valves 6 and 20 have an enlarged structure shown in FIG. 5 and are formed in a disk shape with a flexible material such as rubber or elastomer, and are provided with three through holes 23. ing. The peripheral portion 24 is fixed, and the front side of the paper is the pressure reducing side and the side pulled by the plunger. An opening 25 exists on the back side of the paper surface, and this opening 25 is located inside the through hole 23 so that the through hole 23 and the opening 25 do not overlap. In this state, when the plunger is pulled to the front side of the paper, the pressure is reduced and the check valves 6 and 20 also expand to the front side. Therefore, the passage 25 closed by the check valves 6 and 20 is opened and the check valve 6 is opened. , 20 and through the through hole 23 to the front side of the mixed specimen paper surface. However, of course, the check valve of the present invention is not limited to this configuration.

図６は本発明の完成品である。前述した実施形態の構造がケーシング２６に内装されている。蓋２７が開閉自在に取り付けられており、血液などの検体を取り入れ口２８から取り入れ、口部１０を通して混合室２に入れることができる。混合室２に血液を入れた後に蓋２７を閉じると混合室２は密閉されるので、混合は蓋２７を閉じて行う。また、第一のノブ２９は液封入シリンダー内プランジャー７と結合されており、この液封入シリンダー内プランジャー７及び液封入シリンダー３を押すときに使用され、第二のノブ３０は採取プランジャー２１に結合されており、この採取プランジャー２１を引くときに使用される。このように、本発明はケーシング２６の中にまとまりよくコンパクトに構成することができ、２つのノブを摺動させるだけの操作で作業を簡単に行うことができる。   FIG. 6 shows the finished product of the present invention. The structure of the above-described embodiment is housed in the casing 26. A lid 27 is attached so as to be openable and closable, and a specimen such as blood can be taken from the intake 28 and can be put into the mixing chamber 2 through the mouth 10. When the lid 27 is closed after the blood is put into the mixing chamber 2, the mixing chamber 2 is sealed. Therefore, the mixing is performed with the lid 27 closed. The first knob 29 is connected to the plunger 7 in the liquid-filled cylinder, and is used when pushing the plunger 7 in the liquid-filled cylinder and the liquid-filled cylinder 3, and the second knob 30 is a sampling plunger. 21 and is used when pulling the collection plunger 21. As described above, the present invention can be configured in a compact and compact manner in the casing 26, and the operation can be easily performed by simply sliding the two knobs.

次に本発明の使用方法を血液ついて説明する。本発明を使用するときは、図７に拡大して示す採血具３１が主に使用されるので、まずこの採血具３１について説明する。粘着シート３２の裏面３４に粘着剤が塗布され、中央部に丸い孔３３が設けられている。シート１は薄く形成されているので絆創膏のような柔軟性があり皮膚に確実に貼り付けることができる。シートの表面には対向する一対の溝３５，３５が平行に延びるように設けられている。別体の容器３６はプラスチックで一体に形成されており、カップ３７の縁に矩形のフランジ３８が設けられている。フランジ３８を溝３５，３５に挿入して容器３６をセットしたときに容器の開口部３９と孔３３は連通する。採血するときは、まず被診断者の指などの皮膚をランセットで穿刺し、その穿刺した部分に孔３３を宛がい、シート３２を皮膚に貼り付ける。適量の血液をカップ３７内に取り入れた後に、シート３２を皮膚に貼り付けたままでカップ３７を本発明のケーシングの取り入れ口２８の縁に宛がい指を移動させる。これにより、容器３６が取り入れ口２８の縁に引っかかったまま残されるので、容器３６のみがシート３２から外れて混合室２に落ちるのである。なお、本発明ではこのような採血具を用いることなく、皮膚からにじみ出た血液を直接混合室２に入れてもよいことは勿論である。   Next, the method of use of the present invention will be described for blood. When the present invention is used, the blood collection tool 31 shown in an enlarged view in FIG. 7 is mainly used. An adhesive is applied to the back surface 34 of the adhesive sheet 32, and a round hole 33 is provided in the center. Since the sheet 1 is thinly formed, the sheet 1 has flexibility such as a bandage and can be reliably attached to the skin. A pair of opposed grooves 35, 35 are provided on the surface of the sheet so as to extend in parallel. The separate container 36 is integrally formed of plastic, and a rectangular flange 38 is provided on the edge of the cup 37. When the container 36 is set by inserting the flange 38 into the grooves 35, 35, the opening 39 and the hole 33 of the container communicate with each other. When blood is collected, first, the skin of a subject's finger or the like is punctured with a lancet, the hole 33 is applied to the punctured portion, and the sheet 32 is affixed to the skin. After taking an appropriate amount of blood into the cup 37, the cup 37 is moved to the edge of the intake port 28 of the casing of the present invention and the finger is moved while the sheet 32 is stuck to the skin. As a result, the container 36 remains on the edge of the intake port 28, so that only the container 36 is detached from the sheet 32 and falls into the mixing chamber 2. In the present invention, it is needless to say that blood exuding from the skin may be directly put into the mixing chamber 2 without using such a blood collection tool.

図２に示すように、混合室２に血液を入れた後に蓋２７を閉じて液封入シリンダー内プランジャー７を押すと、逆止め弁６が開いて空所９内の加え液が混合室２内に流される。実際の完成品では第一のノブ２９を摺動させることにより行う。液封入シリンダー内プランジャー７の先端が逆止め弁６に当たるまで液封入シリンダー内プランジャー７を押すが、当たったことは指先の感触あるいはケーシング２６や液封入シリンダー３を透明部材で形成するなどの方法で確認できる。その際、液封入シリンダー内プランジャーのフランジ４０が液封入シリンダーのフランジ４１にも同時に当たる構成としてもよい。この後に、混合室２内で血液と加え液を混合し、図３に示すようにさらに液封入シリンダー内プランジャー７を押すことによって液封入シリンダー３も同様に前進し、希釈血液を通路１７を介して貯留室１１に送る。希釈血液が送られたことによりピストン１８は貯留シリンダー１２の端方向に擦動する。   As shown in FIG. 2, after the blood is put into the mixing chamber 2, the lid 27 is closed and the plunger 7 in the liquid-sealed cylinder is pushed, so that the check valve 6 is opened and the added liquid in the space 9 is mixed with the mixing chamber 2. Shed inside. In an actual finished product, the first knob 29 is slid. The plunger 7 in the liquid-filled cylinder is pushed until the tip of the plunger 7 in the liquid-filled cylinder hits the check valve 6. It can be confirmed by the method. At this time, the flange 40 of the plunger in the liquid-filled cylinder may be configured to simultaneously contact the flange 41 of the liquid-filled cylinder. Thereafter, the blood and the liquid are mixed in the mixing chamber 2, and as shown in FIG. 3, by further pushing the plunger 7 in the liquid sealing cylinder, the liquid sealing cylinder 3 advances in the same manner, and the diluted blood passes through the passage 17. To the storage chamber 11. The piston 18 is rubbed in the direction of the end of the storage cylinder 12 when the diluted blood is sent.

次に図４に示すように、採取プランジャー２１を引いてフィルター１９で血漿を分離し採取室１３に送る。血漿を取り出すときは、採取シリンダー１４を取り外すことなどにより容易に取り出すことができる。   Next, as shown in FIG. 4, the collection plunger 21 is pulled to separate the plasma with the filter 19 and send it to the collection chamber 13. When taking out the plasma, it can be taken out easily by removing the collection cylinder 14 or the like.

本発明は血液の血漿成分を採取する場合に限定されないことは勿論であって、あらゆる検体に加え液を加えてその液状成分を採取する場合に用いられる。例えば、粉末状物質や土壌などのような検体の診断あるいは分析等に使用することができる。   Of course, the present invention is not limited to the collection of blood plasma components, and is used to collect liquid components by adding liquid to any specimen. For example, it can be used for diagnosis or analysis of a specimen such as a powdery substance or soil.

使用前の本発明の断面図Cross section of the present invention before use 加え液を混合室に送った後の断面図Sectional view after feeding the additive liquid to the mixing chamber 加え液を貯留室に送った後の断面図Sectional view after feeding the additive liquid to the storage chamber 加え液を採取室に送った後の断面図Sectional view after sending the added liquid to the collection chamber 逆止め弁の拡大正面図Enlarged front view of check valve 本発明の完成品の斜視図Perspective view of the finished product of the present invention 採血具の拡大斜視図Enlarged perspective view of blood collection tool

符号の説明Explanation of symbols

１ 混合装置
２ 混合室
３ 液封入シリンダー
４ シール
５ 開口部
６ 逆止め弁
７ 液封入シリンダー内プランジャー
８ シール
９ 空所
１０ 口部
１１ 貯留室
１２ 貯留シリンダー
１３ 採取室
１４ 採取シリンダー
１５ 混合シリンダー
１６ 分離装置
１７ 通路
１８ ピストン
１９ フィルター
２０ 逆止め弁
２１ 採取プランジャー
２２ シール
２３ 透孔
２４ 周辺部
２５ 開口部
２６ ケーシング
２７ 蓋
２８ 取り入れ口
２９ 第一のノブ
３０ 第二のノブ
３１ 採血具
３２ シート
３３ 孔
３４ シートの裏面
３５ 溝
３６ 容器
３７ カップ
３８ フランジ
３９ 開口部
４０ フランジ
４１ フランジ DESCRIPTION OF SYMBOLS 1 Mixing apparatus 2 Mixing chamber 3 Liquid enclosure cylinder 4 Seal 5 Opening part 6 Check valve 7 Plunger in liquid enclosure cylinder 8 Seal 9 Cavity 10 Mouth part 11 Reservoir chamber 12 Reservation cylinder 13 Collection chamber 14 Collection cylinder 15 Mixing cylinder 16 Separation device 17 Passage 18 Piston 19 Filter 20 Check valve 21 Collection plunger 22 Seal 23 Through hole 24 Peripheral portion 25 Opening portion 26 Casing 27 Lid 28 Intake port 29 First knob 30 Second knob 31 Blood collection tool 32 Sheet 33 Hole 34 Sheet back surface 35 Groove 36 Container 37 Cup 38 Flange 39 Opening 40 Flange 41 Flange

Claims (1)

検体と加え液を混合する混合構造と、検体と加え液を混合した混合検体から液状成分を分離するための分離構造を含み、前記混合構造は検体と加え液を混合するための混合室を有し、前記分離構造は貯留室と採取室を有し、貯留室は混合室と連通し、採取室はフィルターを介して貯留室と連通し、且つ、混合室内の混合検体を貯留室に送るための手段と、貯留室に送られた混合検体を前記フィルターに通し液状成分を分離して採取室に送る手段とを備えており、
前記混合構造は、加え液を蓄えた液封入シリンダーを有し、この液封入シリンダーの先端に設けられた開口部により液封入シリンダーと混合室が連通し、且つ前記開口部は閉塞部材で閉塞され、液封入シリンダー内プランジャーと前記閉塞部材との間の空所に加え液が蓄えられており、前記液封入シリンダー内プランジャーを押すことによって閉塞部材が開通し、加え液が混合室に送られ、
前記混合室の内壁はシリンダー壁状に形成され、液封入シリンダーが混合室内を擦動できるように装着されており、液封入シリンダーを押すことによって混合検体が混合室から前記貯留室に送られ、
液封入シリンダー内プランジャーの後端は液封入シリンダーの後端よりも後方に突き出ており、
貯留室の内壁はシリンダー壁状に形成されその貯留室内にピストンが装着されており、混合検体は混合室から前記ピストンとフィルターの間の部分に送られ、混合検体の圧力によってピストンはフィルターと反対方向に擦動して混合検体は貯留室内に入り、
採取室の内壁はシリンダー壁状に形成され採取プランジャーが装着されており、採取プランジャーを引くことによりフィルターを介して貯留室内の液状成分を採取室内に送ることを特徴とする分離装置。 A mixing structure for mixing the specimen and the added liquid, and a separation structure for separating the liquid component from the mixed specimen obtained by mixing the specimen and the added liquid, and the mixing structure has a mixing chamber for mixing the specimen and the added liquid. The separation structure has a storage chamber and a collection chamber, the storage chamber communicates with the mixing chamber, the collection chamber communicates with the storage chamber through a filter, and the mixed specimen in the mixing chamber is sent to the storage chamber And means for passing the mixed specimen sent to the storage chamber through the filter to separate the liquid component and sending it to the collection chamber,
The mixing structure has a liquid-filled cylinder that stores added liquid, the liquid-filled cylinder and the mixing chamber communicate with each other through an opening provided at the tip of the liquid-filled cylinder, and the opening is closed by a closing member. In addition, liquid is stored in the space between the plunger in the liquid-sealed cylinder and the closing member, and the closing member is opened by pushing the plunger in the liquid-sealed cylinder, and the added liquid is sent to the mixing chamber. And
The inner wall of the mixing chamber is formed in a cylinder wall shape, and a liquid-filled cylinder is mounted so that the liquid-filled cylinder can be rubbed in the mixing chamber, and the mixed specimen is sent from the mixing chamber to the storage chamber by pushing the liquid-filled cylinder.
The rear end of the plunger in the liquid cylinder protrudes backward from the rear end of the liquid cylinder,
The inner wall of the storage chamber is formed in the shape of a cylinder wall, and a piston is mounted in the storage chamber. The mixed sample is sent from the mixing chamber to the portion between the piston and the filter, and the piston is opposed to the filter by the pressure of the mixed sample. The mixed specimen enters the storage chamber by rubbing in the direction,
A separation apparatus, wherein an inner wall of a collection chamber is formed in a cylinder wall shape and a collection plunger is attached, and a liquid component in a storage chamber is sent to the collection chamber through a filter by pulling the collection plunger.
JP2004226137A 2004-08-02 2004-08-02 Separation device for mixing sample and added liquid to separate liquid components Active JP3981105B2 (en)

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WO2018216607A1 (en) * 2017-05-22 2018-11-29 Okinawa Institute Of Science And Technology School Corporation Integrated system for sampling and processing a liquid suspension

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CN103884654B (en) * 2012-02-28 2017-02-15 何毅 Reagent prepackaging cuvette structure

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018216607A1 (en) * 2017-05-22 2018-11-29 Okinawa Institute Of Science And Technology School Corporation Integrated system for sampling and processing a liquid suspension

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