JP2564406B2 - Water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes, and method for producing the same - Google Patents
Water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes, and method for producing the sameInfo
- Publication number
- JP2564406B2 JP2564406B2 JP1291753A JP29175389A JP2564406B2 JP 2564406 B2 JP2564406 B2 JP 2564406B2 JP 1291753 A JP1291753 A JP 1291753A JP 29175389 A JP29175389 A JP 29175389A JP 2564406 B2 JP2564406 B2 JP 2564406B2
- Authority
- JP
- Japan
- Prior art keywords
- water
- soluble paper
- microorganism
- plant
- parasitic nematodes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Paper (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、植物病原菌又は植物寄生性線虫防除用水溶
紙及びその製造法に関する。TECHNICAL FIELD The present invention relates to a water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes and a method for producing the same.
従来、土壌伝染性植物疾病及び植物寄生性線虫の防除
には、クロルピクリン、臭化メチル、Ｄ−Ｄ、EDB、DBC
P等の土壌くん蒸剤が使用されていたが、これらの土壌
くん蒸剤は、環境汚染や人畜への影響があることが知ら
れるようになったほか、これらで薬剤処理しても土壌で
は病原菌や線虫の密度の復活が早いことも明らかになっ
てきた。Conventionally, for the control of soil-borne plant diseases and plant parasitic nematodes, chlorpicrin, methyl bromide, DD, EDB, DBC
Soil fumigants such as P were used, but it became known that these soil fumigants had an effect on environmental pollution and humans and animals. It has also become clear that the density of nematodes is quickly revived.
一方、水溶紙は、機密文書用紙、ラベル等の筆記用
紙、印刷用紙として、または使用後溶解流去させる産業
用資材として、あるいは婦人、乳幼児の衛生材用紙とし
て主に用いられている。On the other hand, water-soluble papers are mainly used as confidential document papers, writing papers such as labels, printing papers, or industrial materials to be dissolved and washed away after use, or as sanitary papers for women and infants.
従って、本発明の目的は、環境を汚染せず、また人畜
に無害で、植物に対して有害な土壌中の生物、即ち、植
物病原菌又は植物寄生性線虫を防除することができる防
除用水溶紙及びその製造方法を提供することにある。Therefore, the object of the present invention is to control the organisms in the soil that are not polluting the environment, are harmless to humans and animals, and are harmful to plants, that is, phytopathogenic fungi or plant parasitic nematodes. It is to provide paper and a method for manufacturing the paper.
本発明者らは、水溶紙の成分であるナトリウムカルボ
キシメチルセロール（CMC−Na）を分解する微生物につ
いて鋭意研究した結果、このような微生物の中に植物病
原菌又は植物寄生性線虫に対して拮抗性を有する微生物
が多く含まれていることを知見した。As a result of diligent research on a microorganism that decomposes sodium carboxymethyl serol (CMC-Na), which is a component of water-soluble paper, the present inventors have found that among such microorganisms, plant pathogens or plant parasitic nematodes It was found that many microorganisms having antagonistic properties are contained.
本発明は、上記知見に基づいてなされたもので、水に
対して速やかに溶解し得る水溶紙と、該水溶紙の面上に
分解された、植物病原菌又は植物寄生性線虫に拮抗性を
有する微生物または該微生物に塩類が加えられた混合物
と、該微生物または該混合物が分散された上記水溶紙に
接合された水溶紙からなり、上記水溶紙は、上記微生物
の栄養源となって該微生物を増殖させるものであること
を特徴とする植物病原菌又は植物寄生性線虫防除用水溶
紙を提供することにより上記目的を達成したものであ
る。The present invention has been made based on the above findings, water-soluble paper that can be rapidly dissolved in water, and decomposed on the surface of the water-soluble paper, antagonistic to plant pathogenic fungi or plant parasitic nematodes. A microorganism having or a mixture of salts added to the microorganism, and a water-soluble paper bonded to the water-soluble paper in which the microorganism or the mixture is dispersed, wherein the water-soluble paper serves as a nutrient source for the microorganism. The above object was achieved by providing a water-soluble paper for controlling phytopathogenic fungi or plant-parasitic nematodes, which is characterized in that it proliferates.
また、本発明は、上記植物病原菌又は植物寄生性線虫
防除用水溶紙を好適に製造することができる方法、即
ち、植物病原菌又は植物寄生性線虫に拮抗性を有する微
生物または該微生物に塩類が加えられた混合物を、水に
対して速やかに溶解し且つ該微生物の栄養源となって該
微生物を増殖させ得る水溶紙の面上に散布し、その上に
該水溶紙を接合することを特徴とする植物病原菌又は植
物寄生性線虫防除用水溶紙の製造法を併せて提供するも
のである。Further, the present invention is a method capable of suitably producing the above-mentioned water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes, that is, a microorganism having antagonistic activity against plant pathogenic fungi or plant parasitic nematodes or salts of the microorganisms. The mixture added with is sprayed on the surface of a water-soluble paper which can be rapidly dissolved in water and serve as a nutrient source for the microorganism to grow the microorganism, and the water-soluble paper is bonded onto the surface. The present invention also provides a method for producing a water-soluble paper for controlling a characteristic plant pathogenic fungus or a plant parasitic nematode.
本発明に用いられる植物病原菌又は植物寄生線虫に拮
抗性を有する微生物（以下「拮抗微生物」と略称する）
は、植物病原菌及び／又は植物寄生性線虫に対して拮抗
性を有しておれば単一の種類からなるものであっても、
二種類以上混合されたものであってもよく、該拮抗微生
物としては、例えば、細菌類、放射菌類、真菌類、粘菌
類、地衣類、藻類などを挙げられる。そして、これらの
拮抗微生物としては、生菌類は勿論のこと、濃縮したも
の、噴霧乾燥したもの、凍結乾燥したもの、凍結融解し
たものなどであってもよい。Microorganisms having antagonistic properties against plant pathogenic fungi or plant parasitic nematodes used in the present invention (hereinafter abbreviated as "antagonist microorganisms")
Is composed of a single species as long as it has antagonistic activity against plant pathogens and / or plant parasitic nematodes,
A mixture of two or more kinds may be used, and examples of the antagonistic microorganisms include bacteria, radiofungi, fungi, slime molds, lichens, and algae. As these antagonistic microorganisms, not only live bacteria but also concentrated ones, spray dried ones, freeze dried ones, freeze thawed ones and the like may be used.
上記拮抗微生物は、常法に従い液体培地で振盪培養ま
たは静止培養を行い、その培養物を液体培地と共にミキ
サーで粉砕し、粉砕された培養物を含有する液体培地を
噴霧乾燥や凍結乾燥で乾燥することによって粉末状にし
て用いられるもの、あるいは上記培養物を液体培地と共
にミキサーで粉砕し、溶液状のまま用いられるものがあ
る。The above-mentioned antagonistic microorganism is shake-cultured or statically cultivated in a liquid medium according to a conventional method, the culture is crushed with a liquid medium in a mixer, and the liquid medium containing the crushed culture is dried by spray drying or freeze drying. There are those which are used in the form of powder by the above, or those which are used as a solution by pulverizing the above-mentioned culture together with a liquid medium by a mixer.
また、上記混合物は、具体的には、粉末状あるいは溶
液状の拮抗微生物に塩類が混合されたものである。該塩
類は上記拮抗微生物の栄養源となるものであればよく、
このような塩類として、例えば、りん酸一カリウム、り
ん酸二カリウム、硫酸マグネシウム、硝酸カリウム、硝
酸ナトリウム、塩化ナトリウム、塩化カリウムなどが挙
げられる。また、上記拮抗微生物と上記塩類との混合比
（重量比）は1/2〜1/5であることが好ましい。Further, the above-mentioned mixture is, specifically, a salt or a mixture of powdery or solution-like antagonistic microorganisms. The salts may be those that serve as a nutrient source for the antagonistic microorganism,
Examples of such salts include monopotassium phosphate, dipotassium phosphate, magnesium sulfate, potassium nitrate, sodium nitrate, sodium chloride, potassium chloride and the like. Further, the mixing ratio (weight ratio) of the antagonistic microorganism and the salts is preferably 1/2 to 1/5.
本発明に用いられる水溶紙は、通常CMC−Naと木材パ
ルプなどからなり、水によって速やかに溶解するもので
あって、CMC−Naと木材パルプなどが上記拮抗微生物の
栄養源となって拮抗微生物を増殖させるようなものであ
ればよい。そして、該水溶紙は通常20〜120g/m2のもの
が好ましい。The water-soluble paper used in the present invention usually consists of CMC-Na and wood pulp, etc., and is one that is rapidly dissolved by water, and CMC-Na and wood pulp etc. serve as nutrient sources for the above-mentioned antagonistic microorganisms. What is necessary is just to multiply. The water-soluble paper is preferably 20 to 120 g / m 2 .
而して、上記拮抗微生物または上記混合物は、上記水
溶紙の面上均一に分散されていることが好ましく、分散
量は、拮抗微生物単独の場合が５〜20g/m2、混合物の場
合が20〜50g/m2であることが好ましい。分散に際し、粉
末状の拮抗微生物はホッパー等の分散手段を用いて上記
水溶紙の面上に分散され、また溶解状のものは上記水溶
紙の面上に塗布することによって分散される。Thus, the antagonistic microorganism or the mixture is preferably uniformly dispersed on the surface of the water-soluble paper, and the dispersion amount is 5 to 20 g / m 2 in the case of the antagonistic microorganism alone, and 20 in the case of the mixture. It is preferably ˜50 g / m 2 . Upon dispersion, the powdery antagonistic microorganisms are dispersed on the surface of the water-soluble paper by using a dispersion means such as a hopper, and the dissolved ones are dispersed by coating on the surface of the water-soluble paper.
また、上記拮抗微生物が分散された水溶紙に水溶紙を
圧着する際には、水溶紙間の接合をよくするために予め
水溶紙の接合面に溶出崩壊しない程度の少量の水を付与
しておくことが好ましい。Further, when pressure bonding the water-soluble paper to the water-soluble paper in which the antagonistic microorganisms are dispersed, in order to improve the bonding between the water-soluble papers, a small amount of water that does not elute and disintegrate is previously added to the bonding surface of the water-soluble paper. It is preferable to set.
而して、本発明の防除用水溶紙は、その内部に含有さ
れる拮抗微生物が植物病原菌（特に土壌伝染性植物病
害）及び／又は植物寄生性線虫に対して強力な防除効果
を示し、しかも拮抗微生物が水溶紙、塩類を栄養源とし
て増殖してその効果をより強力に発揮する。従って、該
防除用水溶紙が土壌に溶解することによって土壌に拮抗
微生物を付与し且つ増殖するため、連作を障害する土壌
病害及び植物寄生性線虫害等の発生を防止し、連作する
ことのできない農作物の連作を可能にすることができ、
農作物を栽培する上で防除用剤として特に有益である。Thus, the control water-soluble paper of the present invention, the antagonistic microorganisms contained therein show a strong control effect against plant pathogens (particularly soil-borne plant diseases) and / or plant parasitic nematodes, Moreover, the antagonistic microorganisms grow using water-soluble paper and salts as nutrient sources, and exert their effects more strongly. Therefore, since the control water-soluble paper dissolves in the soil to impart and proliferate an antagonistic microorganism to the soil, it prevents the occurrence of soil diseases and plant parasitic nematode damage that hinder the continuous cropping, and the continuous cropping cannot be performed. It can enable continuous crop production,
It is particularly useful as a control agent for cultivating agricultural products.
尚、本発明に用いられる拮抗微生物の培養は、上述の
液体培養は勿論、固体培養したものであってもよい。The antagonistic microorganism used in the present invention may be solid-cultured as well as the liquid culture described above.
次に、本発明の植物病原菌又は植物寄生性線虫防除用
水溶紙を製造する方法について第１図を参照しながら説
明する。尚、第１図は、本発明方法実施するに好適に用
いられる製造装置出である。Next, a method for producing the water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes of the present invention will be described with reference to FIG. It should be noted that FIG. 1 shows the production apparatus suitably used for carrying out the method of the present invention.
本発明の防除用水溶紙を製造する装置は、水溶紙１を
巻き出す第１巻き出し部２と該第１巻き出し部２に対し
て所定間隔を隔てた位置で上記水溶紙１を巻き取る巻き
取り部３とを備えている。また、該製造装置は、上記第
１巻き出し部２の稍々下流に位置し、上方から拮抗微生
物または混合物（以下、「拮抗微生物」で代表する）４
を上記水溶紙１の面上に散布するホッパー５と、該ホッ
パー５の下流側に位置し、上記拮抗微生物４が散布され
た水溶紙１の面上に水溶紙６を巻き出す第２巻き出し部
７と、該第２巻き出し部７から巻き出された水溶紙６を
第１巻き出し部２から巻き出された水溶紙１の面上に重
ね合わせる上下一対のロール８、８と、該ロール８、８
と上記巻き取り部３の間に位置し、重ね合わされた２枚
の水溶紙１、６を所定の圧力で圧着する上下一対の圧着
ロール９、９とを備えている。尚、10は水溶紙６の下面
に水を散布する水供給部である。An apparatus for producing water-soluble paper for control according to the present invention winds up the water-soluble paper 1 at a first unwinding portion 2 for unwinding the water-soluble paper 1 and at a position spaced apart by a predetermined distance from the first unwinding portion 2. The winding unit 3 is provided. Further, the manufacturing apparatus is located downstream of the first unwinding section 2 and is arranged from above in an antagonistic microorganism or mixture (hereinafter, represented by “antagonist microorganism”) 4
A hopper 5 for spraying the water-soluble paper 1 on the surface of the water-soluble paper 1, and a second unwinding located on the downstream side of the hopper 5 for unwinding the water-soluble paper 6 on the surface of the water-soluble paper 1 on which the antagonistic microorganisms 4 are sprayed. Part 7, a pair of upper and lower rolls 8 and 8 for superposing the water-soluble paper 6 unwound from the second unwinding part 7 on the surface of the water-soluble paper 1 unwound from the first unwinding part 2, Roll 8, 8
And a pair of upper and lower pressure-bonding rolls 9 and 9 which are positioned between the winding section 3 and pressure-bond the two water-soluble papers 1 and 6 which are superposed on each other at a predetermined pressure. Incidentally, 10 is a water supply unit for spraying water on the lower surface of the water-soluble paper 6.
次に、上記製造装置を用いた本発明方法の一実施態様
について説明すると、水溶紙１が第１巻き出し部２から
巻き出されると、拮抗微生物４がホッパー５によって水
溶紙１の面上に散布される。拮抗微生物が散布された水
溶紙１が流下すると第２巻き出し部７から巻き出された
水溶紙６は水供給部10によって下面に水が付与された状
態でロール８、８によって重ね合わされる。次いで、圧
着ロール９、９によってこれら両水溶紙１、６が拮抗微
生物４を含有された状態で圧着されて一体化し、本発明
の防除用水溶紙となって巻き取り部３に巻き取られる。Next, one embodiment of the method of the present invention using the above-mentioned manufacturing apparatus will be described. When the water-soluble paper 1 is unwound from the first unwinding section 2, the antagonistic microorganisms 4 are hopper 5 to the surface of the water-soluble paper 1. It is scattered. When the water-soluble paper 1 sprayed with the antagonistic microorganisms flows down, the water-soluble paper 6 unwound from the second unwinding section 7 is superposed by the rolls 8 and 8 with the water supplied to the lower surface by the water supply section 10. Next, the two water-soluble papers 1 and 6 are pressure-bonded by the pressure-bonding rolls 9 and 9 in a state of containing the antagonistic microorganisms 4 to be integrated, and become the control water-soluble paper of the present invention and are wound up on the winding section 3.
次に、下記実施例に基づいて本発明を説明するが、本
発明はこれらの実施例によって何等制限されるものでは
ない。Next, the present invention will be described based on the following examples, but the present invention is not limited by these examples.
実施例１ ジャガイモ煎汁液体培地に水溶紙〔商品名デイゾルボ
（30MDP180WA）三島製紙（株）製〕を0.1％添加して、
植物病原菌に拮抗性を有する微生物であるトリコデルマ
・ビリデ（Trichoderma viride）、及び植物寄生性線虫
に拮抗性を有する微生物である、アルスロボトリス・オ
リゴスポラ（Arthrobotrys oligospora）（IFO−943
6）、ダクティレラ菌（Dactylella sp）の培養を行っ
た。Example 1 To a potato broth liquid medium was added 0.1% of water-soluble paper [trade name: Disorbo (30MDP180WA) manufactured by Mishima Paper Co., Ltd.],
Trichoderma viride, a microorganism having antagonistic activity against plant pathogenic fungi, and Arthrobotrys oligospora (IFO-943), a microorganism having antagonistic activity against plant parasitic nematodes.
6), Cultivation of Dactylella sp.
また、これとは別に上記水溶紙が無添加である以外は
全で同一の条件にしたジャガイモ煎汁液体培地で上記微
生物の培養を行った。Separately, the above microorganisms were cultured in a potato decoction liquid medium under the same conditions except that the water-soluble paper was not added.
然る後、培養されたそれぞれの微生物の菌体重を測っ
て得られた結果を下記第１表に示した。After that, the results obtained by measuring the bacterial weight of each cultured microorganism are shown in Table 1 below.
下記第１表に示す結果によれば、水溶紙が添加された
培地における微生物は水溶紙が無添加の培地よりも明ら
かに微生物を増殖させることが判る。The results shown in Table 1 below show that the microorganisms in the medium to which the water-soluble paper is added clearly grow the microorganisms more than the medium to which the water-free paper is not added.
実施例２ PDA及び0.1％の水溶紙が添加されたPDAをそれぞれシ
ャーレ１枚当り20ml流し込み、線虫捕食能を有するダク
ティレラ菌（Dactylella sp）を植え付けて25℃で14日
間培養を行った。 Example 2 20 ml of each PDA containing PDA and 0.1% water-soluble paper was poured into each petri dish, dactylella sp. Having nematode-preserving ability was inoculated and cultured at 25 ° C for 14 days.
然る後、平板上の菌叢を１×1cmに切り抜いて菌糸中
に形成された線虫捕捉環状器官数を数えた。After that, the lawn on the plate was cut out into 1 × 1 cm, and the number of nematode-trapping annular organs formed in the hypha was counted.
その結果、0.1％の水溶紙が添加されたPDAでは55±2.
5の捕捉器官が認められたのに対し、PDAでは22±２の捕
捉器官の形成しか認められず、水溶紙が線虫の捕捉活性
を高めることが判る。As a result, 55 ± 2 for PDA with 0.1% water-soluble paper.
While 5 trapping organs were observed, only 22 ± 2 trapping organs were formed in PDA, indicating that water-soluble paper enhances nematode trapping activity.
実施例３ 0.1％の水溶紙が添加されたジャガイモ煎汁液体培地
を100mlずつ500mlの坂口フラスコに分注、殺菌後、ダク
ティレラ菌（Dactylella sp）を接種し、28℃にて振盪
培養を行った。Example 3 Potato decoction liquid medium supplemented with 0.1% water-soluble paper was dispensed in 100 ml portions to a 500 ml Sakaguchi flask, sterilized, inoculated with Dactylella sp, and shake cultured at 28 ° C. .
その一週間後、培養物をガーゼで濾過し菌体を集め、
１フラスコ当り、１％グルタミン酸ナトリウム、10％ス
キムミルクの混合液を5ml加えて、真空凍結乾燥によ
り、乾燥菌体を得た。One week later, the culture was filtered with gauze to collect the cells,
5 ml of a mixed solution of 1% sodium glutamate and 10% skim milk was added to one flask, and vacuum freeze-drying was performed to obtain dry cells.
実施例４ コメヌカ抽出液（コメヌカ100gに水1.5を添加し、1
20℃で20分間加圧蒸煮したのち、ガーゼを濾布として濾
過したもの）１にリン酸一カリウム0.1％、硫酸マグ
ネシウム0.05％加えて、実施例３と同じように分注、殺
菌を行った。Example 4 Rice bran extract (1.5 g of water was added to 100 g of rice bran)
After pressure steaming for 20 minutes at 20 ° C., 0.1% of monopotassium phosphate and 0.05% of magnesium sulfate were added to 1) filtered with gauze as a filter cloth, and dispensed and sterilized in the same manner as in Example 3. .
その後、土壌伝染性植物病原菌であるフザリウム菌
（Fusarium spp）に拮抗性を有するバチルス・ライケニ
ホルミス（Bacillus licheniformis）、トリコデルマ・
ビリデ（Trichoderma viride）及びアスペルギルス・テ
ルリウス（Aspergillus terreus）を接種し、28℃で振
盪培養を行った。After that, Bacillus licheniformis and Trichoderma, which are antagonistic to Fusarium spp, a soil-borne plant pathogen
Viride (Trichoderma viride) and Aspergillus terreus were inoculated and shake culture was performed at 28 ° C.
その一週間後、トリコデルマ・ビリデ及びアスペルギ
ルス・テルリウスは、天竺布で濾過し、濾布上に得られ
た菌体を、また、バチルス・ライケニホルミスは10,00r
pmで10分間遠沈して得られた沈澱物をそれぞれ真空凍結
乾燥し乾燥菌体物を得た。One week later, Trichoderma viride and Aspergillus tellurius were filtered with a cloth for the weight of the cells, and the bacterial cells obtained on the filter cloth, and Bacillus reikeniformis at 10,00r.
The precipitates obtained by centrifugation at pm for 10 minutes were freeze-dried under vacuum to obtain dried bacterial cells.
実施例５ 実施例３及び実施例４の方法で調製した拮抗微生物を
粉末にし、第１図に示す製造装置を用いて下記のように
してそれぞれの植物病原菌又は植物寄生性線虫防除用水
溶紙を得た。Example 5 The antagonistic microorganisms prepared by the methods of Examples 3 and 4 were made into powder, and the water-soluble paper for controlling the respective plant pathogenic fungi or plant parasitic nematodes was prepared as follows using the production apparatus shown in FIG. Got
ホッパーから均一に一定量（5g/m2〜50g/m2）の拮抗
微生物または拮抗微生物と塩類の混合物を適量水溶紙上
に塗布し、片面に5g/m2〜0.1g/m2の水を塗布した坪量30
g/m2の水溶紙を重ねて圧着機により2kg/cm2〜50kg/cm2
の圧力で圧着して上記防除用水溶紙を作成する。Apply a uniform amount (5g / m 2 to 50g / m 2 ) of antagonistic microorganisms or a mixture of antagonistic microorganisms and salts from a hopper onto a suitable amount of water-soluble paper, and apply 5g / m 2 to 0.1g / m 2 of water on one side. Applied basis weight 30
overlapping water sheets of g / m 2 by crimping machine 2kg / cm 2 ~50kg / cm 2
The above-mentioned control water-soluble paper is prepared by pressure bonding under the pressure of.
実施例６ 実施例５と同じ方法で作成した植物寄生性線虫に拮抗
性を有するダクティレラ菌（Dactylella sp）10g/m2を
含有する防除用水溶紙を作成した。Example 6 A control aqueous solution paper was prepared in the same manner as in Example 5 containing 10 g / m 2 of Dactylella sp having antagonistic activity against plant parasitic nematodes.
次いで、該防除用水溶紙を用いて根コブ線虫に対する
防除効果を調べた。即ち、根コブ線虫汚染土を直径15cm
の素焼鉢に８分まで詰めて、その上に100cm2に切った拮
抗微生物を含有する上記水溶紙を敷き、その上にさらに
線虫汚染土を覆土してキュウリを播種し、40日後に根を
掘り取り根コブ線虫の形成数を測定し、その形成数の多
寡により防除効果を調べた。その結果を示したものが下
記第２表である。Next, the control effect against root-knot nematodes was examined using the water-soluble control paper. That is, the root-knot nematode-contaminated soil has a diameter of 15 cm.
After filling up to 8 minutes in a biscuit pot, lay the above water-soluble paper containing 100 cm 2 of antagonistic microorganisms on it, cover the soil with nematode-contaminated soil and sow the cucumbers 40 days later. The number of root-knot nematodes formed was dug and the control effect was examined by the number of the formation. The results are shown in Table 2 below.
下記第２表に示す結果によれば、本実施例の防除用水
溶紙を用いた供試植物１本当りのコブ数は、対照のそれ
よりも格段に少ないことが判る。From the results shown in Table 2 below, it can be seen that the number of lumps per test plant using the water-soluble control paper of this example is significantly smaller than that of the control.
実施例７ 実施例５と同様にして調製したトリコデルマ、バチル
ス及びアスペルギルスの各拮抗微生物10g/m2を含有する
防除用水溶紙を作成した。 Example 7 A control aqueous solution paper containing 10 g / m 2 of each Trichoderma, Bacillus and Aspergillus antagonistic microorganism prepared in the same manner as in Example 5 was prepared.
次いで、該防除用水溶紙を用いてキュウリつる割病に
対する防除効果を調べた。即ち、キュウリつる割病菌を
振盪培養し洪積層土壌に乾土1g当り２〜３×105の菌数
になるように接種し、実施例６と同じ方法で試験を行っ
てその防除効果の判定を萎ちょう枯死個体数で行うこと
によって防除効果を調べた。その結果を示したものが第
２図である。尚、本実施例では１区10ポットを用い、１
ポットに10粒ずつキュウリの種子を播種し３回反復して
行った。また、第２図に示した数値は播種後21日経過後
に調査して得られたものである。Next, the control effect against cucumber wilt disease was investigated using the control water-soluble paper. That is, cucumber wilt disease-causing bacteria was shake-cultured, inoculated into dipping soil so that the number of bacteria was 2 to 3 × 10 5 per 1 g of dry soil, and the test was carried out by the same method as in Example 6 to determine its control effect. The control effect was investigated by carrying out the withering withered population. FIG. 2 shows the result. In this example, 10 pots in 1 ward were used and 1 pot was used.
Ten seeds of cucumber were sown in pots and repeated three times. The values shown in FIG. 2 were obtained by investigating 21 days after sowing.
第２図に示す結果によれば、本実施例の防除用水溶紙
を用いた土壌における発病率は、無処理の土壌における
発病率よりも格段に低下していることが判る。From the results shown in FIG. 2, it is found that the disease incidence in the soil using the control water-soluble paper of this example is much lower than that in the untreated soil.
本発明の製造法によって得られる植物病原菌又は植物
寄生性線虫防除用水溶紙によれば、環境を汚染せず、ま
た人畜に無害で、植物に対して有害な土壌中の生物、即
ち、植物病原菌又は植物寄生性線虫を防除することがで
きる。According to the water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes obtained by the production method of the present invention, it does not pollute the environment, is harmless to humans and animals, organisms in soil harmful to plants, that is, plants. It is possible to control pathogenic fungi or plant parasitic nematodes.
第１図は、本発明の植物病原菌又は植物寄生性線虫防除
用水溶紙の製造法に好適に用いられる装置の概念を示す
側面図、第２図は本発明の各種防除用水溶紙を用いた土
壌と土壌における発病率の関係を示すグラフである。 １、6;水溶紙 4;拮抗微生物（または混合物）FIG. 1 is a side view showing the concept of an apparatus suitably used in the method for producing a water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes according to the present invention, and FIG. It is a graph which shows the relationship between the soil and the disease incidence in soil. 1, 6; water-soluble paper 4; antagonistic microorganism (or mixture)
───────────────────────────────────────────────────── フロントページの続き (72)発明者 大川 元洋 静岡県沼津市西間門226―３ ─────────────────────────────────────────────────── ─── Continued Front Page (72) Inventor Motohiro Okawa 226-3 Nishimamon, Numazu City, Shizuoka Prefecture
Claims (2)
該水溶紙の面上に分散された、植物病原菌又は植物寄生
性線虫に拮抗性を有する微生物または該微生物に塩類が
加えられた混合物と、該微生物または該混合物が分散さ
れた上記水溶紙に接合された水溶紙とからなり、上記水
溶紙は、上記微生物の栄養源となって該微生物を増殖さ
せるものであることを特徴とする植物病原菌又は植物寄
生性線虫防除用水溶紙。1. A water-soluble paper which can be rapidly dissolved in water,
Dispersed on the surface of the water-soluble paper, a microorganism having antagonistic activity against phytopathogenic fungi or plant-parasitic nematodes, or a mixture in which salts are added to the microorganism, and the water-soluble paper in which the microorganism or the mixture is dispersed. A water-soluble paper for controlling phytopathogenic fungi or plant parasitic nematodes, which comprises a bonded water-soluble paper, which serves as a nutrient source for the microorganism to grow the microorganism.
有する微生物または該微生物に塩類が加えられた混合物
を、水に対して速やかに溶解し且つ該微生物の栄養源と
なって該微生物を増殖させ得る水溶紙の面上に散布し、
その上に該水溶紙を接合することを特徴とする植物病原
菌又は植物寄生性線虫防除用水溶紙の製造法。2. A microorganism having antagonistic activity against plant pathogenic fungi or plant parasitic nematodes, or a mixture of the microorganism and salts added thereto is rapidly dissolved in water and serves as a nutrient source for the microorganism. On the surface of water-soluble paper that can grow
A method for producing a water-soluble paper for controlling phytopathogenic fungi or plant-parasitic nematodes, which comprises bonding the water-soluble paper thereon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1291753A JP2564406B2 (en) | 1989-11-09 | 1989-11-09 | Water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes, and method for producing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1291753A JP2564406B2 (en) | 1989-11-09 | 1989-11-09 | Water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes, and method for producing the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03152299A JPH03152299A (en) | 1991-06-28 |
| JP2564406B2 true JP2564406B2 (en) | 1996-12-18 |
Family
ID=17772970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1291753A Expired - Lifetime JP2564406B2 (en) | 1989-11-09 | 1989-11-09 | Water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes, and method for producing the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2564406B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9380366B2 (en) | 2011-11-09 | 2016-06-28 | Yeil Electronics Co., Ltd. | Securing structure of sound output mechanism and sound processing means of mobile device capable of outputting visual and sound signals |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4625626B2 (en) * | 2003-11-27 | 2011-02-02 | 片倉チッカリン株式会社 | Method for suppressing plant parasitic nematode damage using nematode damage-controlling microorganisms and nematode damage-controlling microorganism materials |
| WO2015029872A1 (en) * | 2013-08-26 | 2015-03-05 | 学校法人上智学院 | Repellant for repelling root-knot nematodes, method for manufacturing same and repelling method using repellant |
| CN112592837B (en) * | 2020-12-21 | 2022-02-18 | 中国科学院微生物研究所 | Aspergillus terreus with inhibition effect on root nematodes and application thereof |
-
1989
- 1989-11-09 JP JP1291753A patent/JP2564406B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9380366B2 (en) | 2011-11-09 | 2016-06-28 | Yeil Electronics Co., Ltd. | Securing structure of sound output mechanism and sound processing means of mobile device capable of outputting visual and sound signals |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03152299A (en) | 1991-06-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kay et al. | Evaluation of fungal antagonists for control of onion white rot in soil box trials | |
| Fett | Naturally occurring biofilms on alfalfa and other types of sprouts | |
| CN107151641B (en) | Brevibacillus laterosporus for inhibiting rhizoctonia solani and application thereof | |
| JP3140430B2 (en) | Bacillus microorganisms and their uses | |
| JPH11253151A (en) | Disease injury controlling agent in raising of seedling of rice | |
| US20170197891A1 (en) | Quantum carrier for improving soil function and method of preparation thereof | |
| CZ302596A3 (en) | Preparation and method of plant disease control | |
| CN105875653A (en) | Microbial pesticide containing beauveria bassiana and preparation method of microbial pesticide | |
| JP2015503548A (en) | Composition for controlling phytopathogenic fungi containing Bacillus subtilis KBC1010 strain and its culture as an active ingredient | |
| WO2020262612A1 (en) | Plant disease control agent and plant disease control method | |
| JP2564406B2 (en) | Water-soluble paper for controlling plant pathogenic fungi or plant parasitic nematodes, and method for producing the same | |
| Cabanillas et al. | Survival of Paecilomyces lilacinus in selected carriers and related effects on Meloidogyne incognita on tomato | |
| CN101008017A (en) | Bacillus subtilis (Ehrenberg)Cohn fermented product and its production method and uses | |
| JP7395257B2 (en) | Pest control material using insect parasitic fungi and pest control method using the same | |
| Grewal | Effects of leaf‐matter incorporation on Aphelenchoides composticola (Nematoda), mycofloral composition, mushroom compost quality and yield of Agaricus bisporus | |
| Lartey et al. | Control of Rhizoctonia solani and cotton seedling disease by Laetisaria arvalis and a mycophagous insect Proisotoma minuta (Collembola) | |
| JP5833893B2 (en) | Sterilization method of organic fertilizer | |
| JPH06247822A (en) | Viable cell-containing formulation and its production | |
| TW200825182A (en) | Novel bacillus bacterium strain capable of degrading/volume-reducing plant residue | |
| CN106967654A (en) | A kind of Methylotrophic bacillus and biological compound seed coating material and application | |
| Grewal et al. | Cost effective pre-treatment of chicken manure for controlllng nematodes and fungal flora in synthetic compost used for the cultivation of Agaricus bisporus (Lange) singer | |
| JPH0499704A (en) | Production of insecticidal/germicidal agent | |
| JPH10506288A (en) | Fungi for biological control of plant diseases Gliocladium catenulatum | |
| JPH06261742A (en) | Production of insect-pathogenic mould and medium for proliferating the same | |
| JPS5823365B2 (en) | Saikin Seisatsu Yuzai Oyobi Sono Seizou Hohou |