JPH11253151A - Disease injury controlling agent in raising of seedling of rice - Google Patents

Disease injury controlling agent in raising of seedling of rice

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Publication number
JPH11253151A
JPH11253151A JP10336500A JP33650098A JPH11253151A JP H11253151 A JPH11253151 A JP H11253151A JP 10336500 A JP10336500 A JP 10336500A JP 33650098 A JP33650098 A JP 33650098A JP H11253151 A JPH11253151 A JP H11253151A
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JP
Japan
Prior art keywords
rice
trichoderma
disease
controlling
atroviride
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP10336500A
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Japanese (ja)
Other versions
JP3691265B2 (en
Inventor
Kazuo Kumakura
和夫 熊倉
Takahiro Kawashima
隆弘 川島
Norimichi Muramatsu
憲通 村松
Takeshi Ichikawa
健 市川
Hiroyuki Iyosumi
浩幸 伊代住
Takahiro Makino
孝宏 牧野
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Kumiai Chemical Industry Co Ltd
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Kumiai Chemical Industry Co Ltd
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Priority to JP33650098A priority Critical patent/JP3691265B2/en
Publication of JPH11253151A publication Critical patent/JPH11253151A/en
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Publication of JP3691265B2 publication Critical patent/JP3691265B2/en
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Expired - Lifetime legal-status Critical Current

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  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

PROBLEM TO BE SOLVED: To obtain the subject controlling agent enabling sure control of bacterial disease such as rice Rhizopus Sp and rice bacterial grain rot which are diseases difficult to control and useful as a new biological agrochemical which is a means replaced for chemical germicide or combinedly used with the chemical germicide by including a specific microorganism belonging to the genus Trichoderma. SOLUTION: This controlling agent is a microorganism belonging to the genus Trichoclerma and having controlling ability on bacterial disease damage and contains a microorganism selected from Trichoderma atroviride, Trichoclerma aureoviride, Trichoderma viride, Trichoderma haruzianum, Trichoderma longibrachiatum, Trichoderma pseudokoningii, Trichoderma polysporum and Trichoderma hamatum as an active ingredient. Trichoderma atroviride SKT-1 fungal strain (FERM P-16510), etc., which is a new separated strain is preferable among the above microorganisms.

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【０００１】[0001]

【発明の属する技術分野】本発明は、イネの育苗時に発
生する細菌性病害に対して防除能を有するトリコデルマ
（Trichoderma)属に属する微生物を有効成分とするイネ
の種子伝染性細菌病防除剤、及び、それを利用すること
によって、イネの育苗時に発生する細菌性病害を防除す
るトータルシステムに関するものである。TECHNICAL FIELD The present invention relates to an agent for controlling seed-borne bacterial diseases of rice, comprising as an active ingredient a microorganism belonging to the genus Trichoderma having an ability to control bacterial diseases that occur during rice seedling raising, Further, the present invention relates to a total system for controlling bacterial diseases occurring during rice seedling raising by using the same.

【０００２】[0002]

【従来の技術】わが国の農業は就農人口の低下により農
作業の機械化など、より省力的な作業技術が求められて
いる。種子消毒による病害防除作業の軽減も省力化の一
手法として近年定着してきている。機械移植のための箱
育苗が普及するとともにイネ幼苗の病害による被害は我
が国の重要な問題となっている。しかしながら、これら
の病害のうち、シュードモナス・グルメ（Pseudomonas
glumae)に起因するイネもみ枯細菌病、シュードモナス
・プランタリ（Pseuomonas plantarii)に起因するイネ
苗立枯細菌病、シュードモナス・アベナエ(Pseudomanas
avenae)に起因するイネ褐条病等の細菌による病害は、
特に防除困難な病害である。2. Description of the Related Art In Japan's agriculture, there is a demand for more labor-saving work techniques such as mechanization of agricultural work due to a decrease in the employment population. Reduction of disease control work by seed disinfection has been established in recent years as a labor-saving method. With the spread of box seedlings for mechanical transplantation, the damage caused by disease of rice seedlings has become an important issue in Japan. However, among these diseases, Pseudomonas gourmet
glumae), rice seedling blight caused by Pseuomonas plantarii, rice seedling blight caused by Pseuomonas plantarii, Pseudomanas
avenae) caused by bacterial diseases such as rice brown streak,
Particularly difficult to control.

【０００３】これらの細菌性病害は種子伝染により発病
することが知られており、病害防除のための薬剤による
種子消毒はイネ栽培において重要な作業の一つとなって
いるが、細菌性病害は種子の段階で防除する必要があ
り、生育期に防除することが困難な病害である。従っ
て、これら病害が発生した苗は本田へ移植することがで
きず、苗箱ごと廃棄せざるを得ず、育苗時に完全に撲滅
せねばならない。イネもみ枯細菌病、イネ苗立枯細菌病
及びイネ褐条病は難防除病害であり、有効な薬剤は少な
く、これらの細菌に有効な薬剤として、現在のところわ
ずかにオキソリニック酸剤や水酸化第二銅などの薬剤が
種子消毒剤として使用されているにすぎない。しかしな
がら、水酸化第二銅などの銅剤は重金属であり、重金属
を用いない薬剤が望まれている。又、オキソリニック酸
剤は、近年、オキソリニック酸に対して感受性の低下し
たイネ苗立枯細菌病菌及びイネ褐条病菌の存在が指摘さ
れており、充分な防除効果が得られない事例がしばしば
発生し、今後増々難防除病害化することが懸念される。
更に、近年、種子消毒剤の使用済廃液の処理が環境汚染
の点で問題化していていることから、環境汚染の少ない
防除資材の開発が望まれている。[0003] It is known that these bacterial diseases are caused by seed transmission, and seed disinfection using chemicals for controlling the disease is one of the important tasks in rice cultivation. It is necessary to control at the stage of the disease, and it is difficult to control during the growing season. Therefore, seedlings in which these diseases have occurred cannot be transplanted to Honda, and must be discarded in the seedling box, and must be completely eradicated during seedling raising. Rice blight wilt, rice seedling wilt and rice brown streak are difficult to control diseases, and few effective drugs are available. Drugs such as cupric are only used as seed disinfectants. However, copper agents such as cupric hydroxide are heavy metals, and agents that do not use heavy metals are desired. In recent years, it has been pointed out that oxolinic acid agents have been found to have rice seedling bacterial wilt disease and rice brown streak disease disease with reduced sensitivity to oxolinic acid, and cases in which sufficient control effect cannot be obtained are often observed. It is feared that the disease will become more difficult to control in the future.
Furthermore, in recent years, the treatment of used waste liquid of a seed disinfectant has become a problem in terms of environmental pollution. Therefore, development of a control material with less environmental pollution is desired.

【０００４】一方、近年になって、化学農薬からより環
境への安全性が高いと想定される微生物の利用（いわゆ
る生物農薬）も提案され、一部は実用化段階に達してき
ている。On the other hand, in recent years, the use of microorganisms (so-called biological pesticides), which are assumed to be more environmentally safe from chemical pesticides, has also been proposed, and some of them have reached the stage of practical use.

【０００５】上述のイネの種子伝染に起因する病害の防
除においても、生物防除に関する研究が行われている。
特開平４−２９５４０７では非病原性のシュードモナス
・グルメがイネもみ枯細菌病の防除に有効なこと、特開
平６−８７７１６では病原性を欠失したエルビニア・カ
ルトボーラーがイネ苗立枯細菌病の防除に有効なことが
開示されている。しかし、これらの技術は非病原性細菌
であり、ともに上述のイネ種子伝染性細菌病害の全てを
防除するには至っておらず、省力化のためには一種の微
生物による同時防除が要望されている。[0005] In controlling disease caused by seed transmission of rice as described above, research on biological control has been conducted.
Japanese Patent Application Laid-Open No. Hei 4-295407 discloses that non-pathogenic Pseudomonas gourmet is effective in controlling bacterial blight of rice, and Japanese Patent Application Laid-Open No. Hei 6-87716 discloses that Erbinia cartoboler, which lacks pathogenicity, is effective in controlling rice seedling blight. It is disclosed that it is effective for control. However, these techniques are non-pathogenic bacteria and have not yet been able to control all of the above-mentioned rice seed infectious bacterial diseases, and simultaneous control by a kind of microorganism is required for labor saving. .

【０００６】また、特開平７−０．２５７１６ではシュ
ードモナス・セバシアに属する細菌がイネ苗床で発生す
る病害防除に有効なこと、特開平９−１２４４２６では
シュードモナス・グラディオリに属する細菌がイネばか
苗病、苗立枯細菌病、もみ枯細菌病の防除に有効なこと
が開示されているが、何れも化学農薬に代替しうる程の
効力を示すには至っていない。健苗育苗はイネ栽培にお
いて最も重要な技術であり、そのためにはイネ育苗中に
発生する病害はほぼ完全に撲滅されなくてはならない。
本発明は、トリコデルマ属に属する微生物を有効成分と
するイネの種子伝染性細菌病防除剤に関するものである
が、糸状菌であるトリコデルマ属菌をイネ種子に適用す
ることによりイネの種子伝染性細菌病害を有効に防除す
ることは、全く知られておらず、従来報告例は認められ
ない。Japanese Patent Application Laid-Open No. 7-0.25716 discloses that bacteria belonging to Pseudomonas sebacia are effective in controlling disease occurring in rice seedbeds, and Japanese Patent Application Laid-Open No. 9-124426 discloses that bacteria belonging to Pseudomonas gradii are rice blight seedlings. Although it is disclosed that it is effective for controlling seedling blight and rice blight bacterial disease, none of them has been shown to be effective enough to substitute chemical pesticides. Healthy seedling raising is the most important technique in rice cultivation, for which the disease that occurs in rice seedlings must be almost completely eradicated.
The present invention relates to a rice seed infectious bacterial disease control agent comprising a microorganism belonging to the genus Trichoderma as an active ingredient, and a rice seed infectious bacterium by applying a fungus, Trichoderma, to a rice seed. Effective control of disease is not known at all, and no reports have been reported so far.

【０００７】[0007]

【発明が解決しようとする課題】前記したように、イネ
もみ枯細菌病、イネ苗立枯細菌病及びイネ褐条病は難防
除病害とされており、合成殺菌剤では充分な防除効果が
得られない事例がしばしば発生している。また、合成殺
菌剤による病害防除は耐性菌の出現によって防除効果が
低下する可能性が高く、その場合新たなる殺菌剤の開発
を必要とする。さらに、従来の種子消毒剤では使用済み
廃液を処理しなければならないという問題がある。すな
わち、使い残した消毒剤自体はもとより、消毒剤容器や
散布機器等の洗浄後に排出される廃液は、消毒剤を含有
しているため、これを土壌に捨てたり河川に捨てたりす
ると、消毒剤で土壌や河川が汚染されることとなり、重
大な環境汚染がひき起される。したがって、これらの廃
液は処理することが必須である。As described above, rice germ blight, rice seedling wilt and rice brown streak are considered to be difficult to control, and a synthetic bactericide can provide a sufficient control effect. Unfortunate cases often occur. In addition, the control of disease with a synthetic fungicide is highly likely to reduce the control effect due to the emergence of resistant bacteria, in which case a new fungicide needs to be developed. Furthermore, the conventional seed disinfectant has a problem that the used waste liquid must be treated. In other words, the waste liquid discharged after washing the disinfectant container and the spraying equipment, etc., as well as the residual disinfectant itself, contains the disinfectant. Soil and rivers will be polluted, causing serious environmental pollution. Therefore, it is essential to treat these waste liquids.

【０００８】本発明は、このような問題を解決し、化学
殺菌剤による防除に代わる手段、あるいは併用する手段
として新しい生物農薬、しかも、難防除病害であるイネ
苗立枯細菌病及びイネもみ枯細菌病等の細菌性病害の確
実な防除を可能とする生物農薬を開発する目的でなされ
たものであり、本発明は、省資源、省力化、環境保全等
につながるものである。[0008] The present invention solves the above problems and provides a new biological pesticide as an alternative to, or in combination with, the control with a chemical bactericide. The purpose of the present invention is to develop a biological pesticide capable of reliably controlling bacterial diseases such as bacterial diseases, and the present invention leads to resource saving, labor saving, environmental conservation, and the like.

【０００９】[0009]

【課題を解決するための手段】本発明者らは、上記目的
を達成するため、特に難防除病害であるイネ細菌性病害
の確実な防除を達成するために、これらの病害の防除に
有効であるだけでなく、種子処理及び土壌処理において
もその有効性が維持されるイネの育苗時病害防除菌を新
規にスクリーニングする必要性を認めた。Means for Solving the Problems The inventors of the present invention have been effective in controlling these diseases in order to achieve the above-mentioned objects, and in particular, to achieve reliable control of rice bacterial diseases which are difficult to control. Not only that, it was recognized that it was necessary to newly screen for disease-controlling fungi at the time of raising seedlings in rice, which maintain their effectiveness in seed treatment and soil treatment.

【００１０】そこで、本発明者らは、鋭意研究をした結
果、イネに対して病原性のないトリコデルマ属に属する
糸状菌が、その菌体をイネ種子に処理することにより、
イネ育苗時の難防除病害とされるイネ苗立枯細菌病及び
イネもみ枯細菌病等の細菌性病害を確実に防除すること
を見出した。また、この防除効果はイネに対して病原性
のないトリコデルマ属菌が有すること、更に、薬剤処理
或いは紫外線照射等により病原性を消失させた菌や薬剤
に耐性を獲得した菌等の変異株においても同様の効果を
有することを見出し、本発明を完成するに至った。以
下、本発明について詳しく説明する。Therefore, the present inventors have conducted intensive studies and found that a filamentous fungus belonging to the genus Trichoderma having no pathogenicity to rice can treat the fungus body with rice seeds.
It has been found that bacterial diseases such as rice seedling blight and rice blight, which are considered to be difficult to control when raising rice seedlings, are surely controlled. In addition, this control effect is exhibited by the fact that Trichoderma spp. Having no pathogenicity to rice has the same effect. Have the same effect, and have completed the present invention. Hereinafter, the present invention will be described in detail.

【００１１】本発明の防除剤に用いる微生物としては、
トリコデルマ（Trichoderma）属に属し、イネに病原性
を示さない微生物が利用できる。好ましくは、トリコデ
ルマ・アトロビリデ（Trichoderma atroviride）、トリ
コデルマ・アウレオビリデ（Trichoderma aureovirid
e）、トリコデルマ・ビリデ（Trichoderma viride）、
トリコデルマ・ハルジアナム（Trichoderma haruzianu
m）、トリコデルマ・ロンギブラキアタム（Trichoderma
longibrachiatum）、トリコデルマ・シュードコニンギ
（Trichoderma pseudokoningii）、トリコデルマ・ポリ
スポラム（Trichoderma polysporum）又はトリコデルマ
・ハマタム（Trichoderma hamatum）に属する微生物で
あり、そのうちでも特に好ましい菌株としては、発明者
らが芝（ノシバ）根圏より分離したトリコデルマ・アト
ロビリデ（Trichoderma atroviride）ＳＫＴ−１菌株、
サラダナ根圏より分離したトリコデルマ・アトロビリデ
（Trichoderma atroviride）ＳＫＴ−２菌株、ＳＫＴ−
１菌株を紫外線照射処理することによってベノミル剤に
高度な耐性を獲得したトリコデルマ・アトロビリデ（Tr
ichoderma atroviride）ＳＫＴ−３菌株が例示される。
ＳＫＴ−１菌株及びＳＫＴ−２菌株は、新規分離株であ
って、それぞれ、工業技術院生命工学工業技術研究所に
ＦＥＲＭ Ｐ−１６５１０、ＦＥＲＭ Ｐ−１６５１１
として寄託されている。また、新規誘導株であるＳＫＴ
−３菌株は、工業技術院生命工学工業技術研究所にＦＥ
ＲＭ Ｐ−１７０２１として寄託されている。The microorganism used in the controlling agent of the present invention includes:
A microorganism that belongs to the genus Trichoderma and does not show pathogenicity to rice can be used. Preferably, Trichoderma atroviride, Trichoderma aureovirid (Trichoderma aureovirid)
e), Trichoderma viride,
Trichoderma haruzianu
m), Trichoderma longibrachiatum
longibrachiatum), a microorganism belonging to Trichoderma pseudokoningii, Trichoderma polysporum or Trichoderma hamatum, and among them, a particularly preferred strain is the rhizosphere of turf (Noshiba). Trichoderma atroviride SKT-1 strain,
Trichoderma atroviride SKT-2 strain, SKT-
Trichoderma atroviride (Tr.) Which acquired high resistance to benomyl by treating one strain with ultraviolet irradiation
ichoderma atroviride) SKT-3 strain.
The SKT-1 strain and the SKT-2 strain are new isolates, and FERM P-16510 and FERM P-16511 were sent to the National Institute of Bioscience and Biotechnology at the National Institute of Advanced Industrial Science and Technology, respectively.
Has been deposited as In addition, a new derivative strain, SKT
-3 strain has been fed to the Institute of Biotechnology,
Deposited as RMP-17021.

【００１２】本発明者らによって新たに分離されたトリ
コデルマ・アトロビリデ（Trichoderma atroviride）Ｓ
ＫＴ−１（FERM P-16510）、トリコデルマ・アトロビリ
デ（Trichoderma atroviride）ＳＫＴ−２（FERM P-165
11）及びトリコデルマ・アトロビリデ（Trichoderma at
roviride）ＳＫＴ−３（ＦＥＲＭ Ｐ−１７０２１）
は、以下の性質を有する。[0012] Trichoderma atroviride S newly isolated by the present inventors.
KT-1 (FERM P-16510), Trichoderma atroviride SKT-2 (FERM P-165)
11) and Trichoderma atroviride (Trichoderma at
roviride) SKT-3 (FERM P-17021)
Has the following properties.

【００１３】１）培地上での性質 ポテトデキストロース培地（ＰＤＡ：ジャガイモ２０
０．０ｇ、グルコース２０．０ｇ、寒天２０．０ｇ、蒸
留水１０００ｍｌ）上及び２％麦芽エキス培地（麦芽エ
キス２０．０ｇ、寒天２０．０ｇ、蒸留水１０００ｍ
ｌ）上での生育は良好で、菌糸伸長は早い、はじめ気生
菌糸少なく白色、しだいに羊毛状の気生菌糸を生じ、分
生子形成にしたがって緑色〜暗緑色となる。1) Properties on culture medium Potato dextrose medium (PDA: potato 20)
0.0g, glucose 20.0g, agar 20.0g, distilled water 1000ml) and 2% malt extract medium (malt extract 20.0g, agar 20.0g, distilled water 1000m)
1) The growth on the above is good, the hyphal elongation is fast, the aerial mycelium is little in the beginning, and a wool-like aerial mycelium is gradually formed.

【００１４】２）形態的性質 分生子柄は気生菌糸より生じ、多くは綿毛状にかたま
る。輪生状あるいは不規則に分枝、各分枝は下方のもの
ほど伸びて分枝をくりかえし、全体として円錐形を呈す
る。各分枝はほぼ直角に分かれて先端はフィアライドに
なる。フィアライドは分生子柄先端に２〜４個（平均３
個）が規則正しく対生または輪生し、フィアライド先端
は細くなる。分生子はフィアライド頂端に塊状に形成さ
れる。球形〜豆球形で表面は平滑であり、ＳＫＴ−１菌
株、ＳＫＴ−３菌株は２．５〜４．０×２．５〜３．５
μｍ、ＳＫＴ−２菌株は３．０〜４．０×２．７〜３．
５μｍである。2) Morphological properties The conidiophores are formed from aerial hyphae and are often fluffy. Branching in a ring-like or irregular manner, each branch grows as the lower one repeats the branches, and exhibits a conical shape as a whole. Each branch is divided almost at right angles and the tip becomes phialid. 2 to 4 phialides at the tip of conidiophores (average 3
) Regularly or counterrotate, and the phialide tip becomes thin. Conidia form in clumps at the top of the phialide. Spherical to pea-shaped and the surface is smooth, and SKT-1 strain and SKT-3 strain are 2.5 to 4.0 × 2.5 to 3.5.
μm, the SKT-2 strain is 3.0 to 4.0 × 2.7 to 3.0.
5 μm.

【００１５】３）生理学的性質 生育温度は１０〜３５℃であり、最適温度は２５℃付近
である。ｐＨ４．０〜８．０の間で生育可能であり、最
適ｐＨは５．０〜７．０である。3) Physiological properties The growth temperature is 10 to 35 ° C, and the optimum temperature is around 25 ° C. It can grow between pH 4.0 and 8.0, with an optimum pH between 5.0 and 7.0.

【００１６】本発明においては、上記したトリコデルマ
・アトロビリデ（Trichoderma atroviride）ＳＫＴ−１
（FERM P-16510）、同ＳＫＴ−２（FERM P-16511）、同
ＳＫＴ−３（FERM P-17021）のほか、下記に示すように
イネに対して病原性がなくトリコデルマ属に属する微生
物、又、薬剤処理あるいは紫外線照射等により病原性を
消失させたり薬剤に耐性を獲得した等トリコデルマ属に
属する微生物であれば、すべての微生物が使用できる：In the present invention, the above-mentioned Trichoderma atroviride SKT-1 is used.
(FERM P-16510), SKT-2 (FERM P-16511), and SKT-3 (FERM P-17021), as well as microorganisms that are not pathogenic to rice and belong to the genus Trichoderma as shown below, In addition, all microorganisms belonging to the genus Trichoderma, such as those that have lost their pathogenicity by drug treatment or irradiation with ultraviolet light or have acquired resistance to drugs, can be used:

【００１７】トリコデルマ・アウレオビリデ（Trichode
rma aureoviride）ＩＦＯ−３１９３２、トリコデルマ
・ハルジアナム（T. haruzianum）ＩＦＯ−３１２９
２；トリコデルマ・ロンギブラキアタム（T. longibrac
hiatum）ＩＦＯ−３１９１９；トリコデルマ・シュード
コニンギ（T. pseudokoningii）ＩＦＯ−３１９２０；
トリコデルマ・ビリデ（T. viride）ＩＦＯ−３１１３
７、トリコデルマ・ポリスポラム（T. polysporum）Ｉ
ＦＯ−９３２２；トリコデルマ・ハマタム（T. hamatu
m）ＩＦＯ−３１２９１。[0017] Trichoderma aureobilide
rma aureoviride) IFO-31932, T. haruzianum IFO-3129.
2: Trichoderma longibrachiatum (T. longibrac
hiatum) IFO-31919; Trichoderma pseudokoningii IFO-31920;
T. viride IFO-3113
7. Trichoderma polysporum I
FO-9322; T. hamatu
m) IFO-3291.

【００１８】本発明においては、イネに病原性を示さな
いトリコデルマ属菌がいずれも使用することができる
（これらのトリコデルマ属菌は、その分生胞子懸濁液に
２４時間浸漬したイネ種子を播種しても何らの病原性も
示さなかった）。このようなトリコデルマ属菌として
は、ふすま等の資材培養、固形培地上での静置培養、液
体培養等の公知の手段で増殖させたものを用いればよ
く、生存細胞が増殖するのであれば特に培地の種類、培
養条件等に制限されることはない。In the present invention, any Trichoderma spp. That do not show pathogenicity to rice can be used (these Trichoderma spp. Seeds rice seeds immersed in a conidia suspension for 24 hours). Did not show any pathogenicity). As such a Trichoderma bacterium, a material culture such as bran, a stationary culture on a solid medium, or a culture grown by a known means such as a liquid culture may be used. There is no limitation on the type of culture medium, culture conditions and the like.

【００１９】本発明で用いる防除剤としては、イネに病
原性を示さないトリコデルマ属菌自体のほか、その懸濁
液ないし培養液、又はその処理物（濃縮物、ペースト
状、乾燥物、希釈物等）を広く包含するものである。本
発明におけるイネの育苗時病害防除剤として用いる場合
には、イネに病原性を示さないトリコデルマ属菌の菌体
又は培養液を単独で用いても良いが、通常は、担体、界
面活性剤、分散剤又は補助剤等を配合して常法により例
えば、粉剤、粒剤、水和剤、顆粒水和剤、フロアブル剤
などの形態に製剤化して使用すると更に好ましい。好適
な担体としては、例えばクレー、タルク、ベントナイ
ト、珪藻土、ホワイトカーボン、カオリン、バーミキュ
ライト、消石灰、珪砂、硫安、尿素等の固体担体が挙げ
られ、界面活性剤及び分散剤としては、例えばアルキル
ベンゼンスルホン酸金属塩、ポリオキシエチレンアルキ
ルアリールエーテル、アルキル硫酸ナトリウム、アルキ
ルナフタレンスルホン酸ナトリウム、ジナフチルメタン
ジスルホン酸ナトリウム、リグニン酸ナトリウム等が挙
げられる。補助剤としては、例えばカルボキシメチルセ
ルロース、ポリオキシエチレングリコール、アラビアゴ
ム、澱粉、乳糖等が挙げられる。Examples of the controlling agent used in the present invention include Trichoderma genus which does not show pathogenicity to rice, its suspension or culture solution, or its processed product (concentrate, paste, dried product, diluted product) Etc.). When used as a rice seedling-raising disease controlling agent in the present invention, the cells or culture solution of Trichoderma spp. That do not show pathogenicity to rice may be used alone, but usually, a carrier, a surfactant, It is more preferable that a dispersant or an auxiliary agent is blended and formulated into a form such as a powder, granule, wettable powder, wettable powder, flowable and the like according to a conventional method. Suitable carriers include, for example, solid carriers such as clay, talc, bentonite, diatomaceous earth, white carbon, kaolin, vermiculite, slaked lime, silica sand, ammonium sulfate, urea, and the like.Examples of surfactants and dispersants include alkylbenzene sulfonic acid. Metal salts, polyoxyethylene alkyl aryl ethers, sodium alkyl sulfates, sodium alkyl naphthalene sulfonates, sodium dinaphthyl methane disulfonate, sodium lignate and the like. Examples of the auxiliary include carboxymethyl cellulose, polyoxyethylene glycol, gum arabic, starch, lactose and the like.

【００２０】次に、本発明の防除剤の使用方法を述べ
る。通常、イネを育苗する場合、発芽程度を揃えるため
に、イネ種籾を一定期間水中に浸すいわゆる浸種作業を
行ない、更に３２℃前後の温度に１日〜２日保って催芽
を行なった後に、育苗土壌を充填した育苗箱に播種した
り、苗床に直接播種したり、育苗土壌のほかの各種育苗
培体（ロックウール等）に播種する。本発明に係る防除
剤は、この一連の作業の内の少なくともひとつの時期
（イネの育苗時）の処理、すなわち、病原菌に感染した
イネ種籾に対して、浸種前、浸種期間中、浸種後又は催
芽期間中に浸漬、噴霧、塗布又は粉衣処理することによ
り発病苗が著しく減少し、優れた防除効果を示す。ま
た、イネ種籾の播種前又は播種後にイネの育苗土壌に潅
注又は混和処理することによっても、発病苗が著しく減
少し、優れた防除効果を示す。使用量としては、製剤の
剤型、適用方法、適用場所、適用すべき病害の種類、所
望の防除効果などに応じて適宜選定されるが、粉剤、粒
剤、或るいは水で希釈する製剤の場合は、トリコデルマ
属菌の胞子濃度が、１０2〜１０9程度、好ましくは１０
4〜１０9の範囲で使用するのが望ましい。Next, the method of using the controlling agent of the present invention will be described. Usually, when raising rice seedlings, in order to equalize the degree of germination, so-called seeding work is performed in which the rice seeds are immersed in water for a certain period of time. The seeds are seeded in a nursery box filled with soil, seeded directly on a nursery bed, or seeded on other seedling cultures (rock wool, etc.) in the seedling soil. The control agent according to the present invention is treated at least at one time in this series of operations (at the time of raising rice), that is, for rice seeds infected with pathogenic bacteria, before sowing, during sowing, after soaking, or By dipping, spraying, applying or dressing during the germination period, the number of diseased seedlings is remarkably reduced, and an excellent control effect is exhibited. Also, by irrigating or mixing the rice seedling soil before or after sowing of the rice seed, the diseased seedlings are remarkably reduced and an excellent control effect is exhibited. The amount to be used is appropriately selected depending on the dosage form of the preparation, the application method, the application place, the type of disease to be applied, the desired control effect, etc., but the preparation is diluted with powder, granules, or water. In this case, the spore concentration of the genus Trichoderma is about 10 2 to 10 9 , preferably 10
4 it is desirable to use at -10 9 range.

【００２１】本発明に係る防除剤は、上記したように病
原菌に感染したイネ種籾やそれを含有する培体に適用す
ることはもちろんのこと、病原菌に感染するおそれがあ
る場合や、それを予防するために健全なイネ種籾や育苗
培体に対しても自由に適用することができる。その際、
本発明に係るトリコデルマ属菌はイネに対して病原性を
示さないので、予防的に適用しても何ら害作用は生じな
い。The control agent according to the present invention can be applied not only to rice seeds infected with pathogenic bacteria or a culture medium containing the same as described above, but also to cases in which there is Therefore, it can be freely applied to healthy rice seeds and seedling cultures. that time,
Since the Trichoderma genus according to the present invention does not show pathogenicity to rice, no harmful effects occur even when applied prophylactically.

【００２２】本発明に係るトリコデルマ属菌は、イネ育
苗時に発生し、防除が困難とされるイネ苗立枯細菌病、
イネもみ枯細病及びイネ褐条病のイネ細菌性病害を確実
に防除することができる。しかも、従来の合成農薬によ
る防除のように耐性菌の出現によって防除効果が低下す
るという問題も解消される点からも、本発明はきわめて
卓越している。The Trichoderma bacterium according to the present invention is generated during rice seedling raising and is difficult to control with respect to rice seedling blight disease.
The rice bacterial wilt of rice blight and brown streak can be surely controlled. Moreover, the present invention is extremely excellent in that the problem that the control effect is reduced due to the emergence of resistant bacteria as in the conventional control by synthetic pesticides is also solved.

【００２３】本発明によれば、トリコデルマ属に属する
イネの育苗時細菌性病害防除菌又はその培養液等防除菌
含有物をイネ種子に付着せしめたり、あるいは、該防除
菌を有効成分とする防除剤でイネ種子を処理することに
よって、病害防除性イネ種子を製造することができる。
また、同様にしてイネ育苗培体をトリコデルマ属菌で処
理、付着、含有せしめることにより、病害防除性イネ育
苗培体を製造することができる。According to the present invention, a rice plant belonging to the genus Trichoderma can be provided with a bacterial disease-controlling bacterium at the time of seedling or a bacterium-containing component such as a culture solution thereof attached to rice seeds, or the bacterium as an active ingredient By treating rice seeds with the agent, disease-controlling rice seeds can be produced.
Similarly, by treating, adhering, and including the rice cultivation medium with Trichoderma sp., A disease-controlling rice cultivation medium can be produced.

【００２４】このようにして製造した病害防除性イネ種
子及び／又はイネ育苗培体を容器に収容することによ
り、育苗箱等のイネ育苗容器が製造できる。本発明によ
って製造されたイネ育苗箱は、自動田植機等各種の用途
に使用可能である。このようなイネ育苗箱においては、
イネ苗の密度が非常に高いため、病害が発生しやすいだ
けでなく、一旦病害が発生するとごく短時間にイネ苗全
体に伝染してしまうという欠点は避けられず、健苗育成
の点で解決策が強く希求されていたのであるが、本発明
によってそれがきわめて効率的に解決されたのである。By storing the thus produced disease-controlling rice seed and / or rice seedling culture in a container, a rice seedling container such as a seedling box can be manufactured. The rice nursery box manufactured according to the present invention can be used for various uses such as an automatic rice transplanter. In such a rice nursery box,
Due to the very high density of rice seedlings, not only is it easy to cause disease, but once the disease occurs, it is inevitable that the disease will be transmitted to the whole rice seedling in a very short time. Although a solution was strongly sought, the present invention solved it very efficiently.

【００２５】以下、本発明を実施例により具体的に説明
するが、本発明はこれらの実施例により限定されるもの
ではない。Hereinafter, the present invention will be described specifically with reference to Examples, but the present invention is not limited to these Examples.

【００２６】[0026]

【実施例１：水和剤】トリコデルマ属菌をＰＤＡ平板培
地で７〜１４日間培養して形成させた分生胞子を８重量
部、珪藻土４０重量部、クレー５０重量部、ジナフタレ
ンジスルホン酸ナトリウム１重量部及びリグニンスルホ
ン酸ナトリウム１重量部を混合乾燥後、粉砕して水和剤
とした。Example 1: Hydrating agent 8 parts by weight of conidia formed by culturing Trichoderma spp. On a PDA plate medium for 7 to 14 days, 40 parts by weight of diatomaceous earth, 50 parts by weight of clay, sodium dinaphthalenedisulfonic acid One part by weight and 1 part by weight of sodium ligninsulfonate were mixed and dried, and then pulverized to obtain a wettable powder.

【００２７】[0027]

【実施例２：粒剤】トリコデルマ属菌をＰＤＡ平板培地
で７〜１４日間培養して形成させた分生胞子を蒸留水に
懸濁して作成したトリコデルマ属菌胞子懸濁液３０重量
部、ラウリルアルコール硫酸エステルのナトリウム塩１
重量部、リグニンスルホン酸ナトリウム１重量部、カル
ボキシメチルセルロース２重量部及びクレーを９０重量
部を均一に混合粉砕する。この混合物を、押出式造粒機
を用いて１４〜３２メッシュの粒状に加工した後、乾燥
して粒剤とした。Example 2: Granules 30 parts by weight of a spore suspension of Trichoderma sp. Prepared by suspending conidia formed by culturing Trichoderma sp. On a PDA plate medium for 7 to 14 days in distilled water, lauryl Alcohol sulfate sodium salt 1
Parts by weight, 1 part by weight of sodium ligninsulfonate, 2 parts by weight of carboxymethylcellulose and 90 parts by weight of clay are uniformly mixed and pulverized. This mixture was processed into granules of 14 to 32 mesh using an extrusion granulator, and then dried to obtain granules.

【００２８】[0028]

【実施例３】表１に示したイネに病原性を示さないトリ
コデルマ菌をＰＤ液体培地で２７℃３日間振とう培養
し、得られた菌体をホモジナイズ後蒸留水に懸濁し、菌
体懸濁液を調製した。この菌体懸濁液に、イネもみ枯細
菌病罹病籾（品種黄金晴、開花期にもみ枯細菌病菌を接
種）を、１５℃で５日間浸漬（浴比１：２）した後、菌
液を捨て３２℃の湿室内に１日間保って催芽させた。育
苗培土を充填した径６ｃｍのプラスチックカップに催芽
種子を播種し、播種後３日間、３０℃の育苗庫内に保
ち、更に２５℃の湿室内で１０日間管理した後に、全苗
について発病の有無を調査し数１により発病苗率を、ま
た数２により防除価を算出した。１区当たりの播種量は
湿籾４ｇ（約８０〜９０粒）、試験は３反復で行なっ
た。判定に当たっては、防除価１００％をＳ、同１００
％未満９５％以上をＡ、同９５％未満９０％以上をＢ、
同９０％未満５０％以上をＣ、同５０％未満をＤとし
た。Example 3 Trichoderma bacteria having no pathogenicity to rice shown in Table 1 were cultured in a PD liquid medium with shaking at 27 ° C. for 3 days, and the obtained cells were homogenized and suspended in distilled water. A suspension was prepared. After immersing rice germ blight-infected paddy (variety Koganebare, inoculated with the germ-kill bacterium at the flowering stage) in the cell suspension at 15 ° C. for 5 days (bath ratio 1: 2), the bacterial solution Was discarded and kept in a moist room at 32 ° C. for 1 day to cause germination. Seeding seeds are seeded in a 6 cm diameter plastic cup filled with seedling cultivation soil, kept in a seedling storage at 30 ° C. for 3 days after sowing, and further controlled in a humidity chamber at 25 ° C. for 10 days. And the disease-causing seedling rate was calculated from Equation 1 and the control value was calculated from Equation 2. The seeding amount per section was 4 g of wet paddy (about 80 to 90 grains), and the test was performed in three repetitions. In the judgment, the control value was 100% for S and 100% for
A is less than 95% and B is 90% or more.
Less than 90% and 50% or more were C, and less than 50% were D.

【００２９】[0029]

【表１】 [Table 1]

【００３０】[0030]

【数１】 (Equation 1)

【００３１】[0031]

【数２】 (Equation 2)

【００３２】結果は表１に示す通り、何れのトリコデル
マ菌も、イネもみ枯細菌病に対して明かな発病抑制効果
を示した。As shown in Table 1, all the Trichoderma fungi showed a clear inhibitory effect on bacterial blight of rice.

【００３３】[0033]

【実施例４】表３に示したイネに病原性を示さないトリ
コデルマ菌をＰＤ液体培地で２７℃、３日間振とう培養
し、得られた菌体をホモジナイズ後蒸留水に懸濁し、菌
体懸濁液を調製した。この菌体懸濁液に、イネ苗立枯細
菌病罹病籾（品種黄金晴、開花期に苗立枯細菌病菌を接
種）を、１５℃で５日間浸漬（浴比１：２）した後、菌
液を捨て３２℃の湿室内に１日間保って催芽させた。育
苗培土を充填した径６ｃｍのプラスチックカップに催芽
種子を播種し、播種後３日間、３０℃の育苗庫内に保
ち、更に２５℃の温室内で７日間管理した後に、全苗に
ついて表２の基準によって発病程度を調査し、数３によ
り発病度を求め、更に無処理区との比から数４により防
除価を算出した。１区当たりの播種量は湿籾４ｇ（約８
０〜９０粒）、試験は３反復で行なった。判定に当たっ
ては、防除価１００％をＳ、同１００％未満９５％以上
をＡ、同９５％未満９０％以上をＢ、同９０％未満５０
％以上をＣ、同５０％未満をＤとした。Example 4 Trichoderma non-pathogenic to rice shown in Table 3 was cultured with shaking in a PD liquid medium at 27 ° C. for 3 days, and the obtained cells were homogenized and suspended in distilled water. A suspension was prepared. After immersing rice seedling-infected bacterial disease-affected paddy (variety Koganebare, inoculated with seedling-killing bacterial pathogen during flowering period) in this cell suspension at 15 ° C for 5 days (bath ratio 1: 2), The bacterial solution was discarded and kept in a moist room at 32 ° C. for one day to cause germination. The seeds are seeded in a plastic cup having a diameter of 6 cm filled with the seedling cultivation soil, kept in a seedling storage at 30 ° C. for 3 days after sowing, and further controlled in a greenhouse at 25 ° C. for 7 days. The disease severity was investigated based on the standard, the disease severity was determined by Formula 3, and the control value was calculated by Formula 4 from the ratio with the untreated plot. The seeding rate per ward is 4 g of wet paddy (about 8
0-90) and the test was performed in triplicate. In the judgment, the control value was 100% for S, 95% or more for less than 100% was A, 90% or more for less than 95% was B, and 50% for less than 90%.
% Or more was C, and less than 50% was D.

【００３４】[0034]

【表２】 [Table 2]

【００３５】[0035]

【数３】 (Equation 3)

【００３６】[0036]

【数４】 (Equation 4)

【００３７】[0037]

【表３】 [Table 3]

【００３８】結果は表３に示す通り、何れのトリコデル
マ菌も、イネ苗立枯細菌病に対して明かな発病抑制効果
を示した。As shown in Table 3, all of the Trichoderma fungi exhibited a clear inhibitory effect on bacterial seedling blight.

【００３９】[0039]

【実施例５】トリコデルマ・アトロビリデＳＫＴ−１菌
株、ＳＫＴ−２菌株及びＳＫＴ−３菌株をＰＤＡ平板培
地上で７〜１４日間培養し、得られた分生胞子を蒸留水
に懸濁し、胞子懸濁液を調製した。この胞子懸濁液或る
いは実施例１で作製したＳＫＴ−１菌株の水和剤を２０
０倍又は２０００倍に希釈した菌液に、イネもみ枯細菌
病罹病籾（品種黄金晴、開花期にもみ枯細菌病を接種）
を、１５℃で５日間浸漬（浴比１：２）した後、菌液を
捨て３２℃の湿室内に１日間保って催芽させた。育苗培
土を充填した径６ｃｍのプラスチックカップに催芽種子
を播種し、播種後３日間、３０℃の育苗庫内に保ち、更
に２５℃の湿室内で１１日間管理した後に、全苗につい
て発病の有無を調査し数１により発病苗率を、また数２
により防除価を算出した。１区当たりの播種量は湿籾４
ｇ（約８０〜９０粒）、試験は３反復で行なった。得ら
れた結果を表４に示した。Example 5 Trichoderma atroviride SKT-1, SKT-2, and SKT-3 strains were cultured on a PDA plate medium for 7 to 14 days, and the obtained conidia were suspended in distilled water and suspended. A suspension was prepared. The spore suspension or the wettable powder of the SKT-1 strain prepared in Example 1 was used for 20 times.
Bacterial solution diluted to 0-fold or 2000-fold, rice paddy with rice blight bacterial disease (variety Koganeharu, inoculated with rice blight bacterial disease at flowering stage)
Was immersed at 15 ° C. for 5 days (bath ratio 1: 2), and the bacterial solution was discarded and kept in a moist room at 32 ° C. for 1 day for germination. Seeding seeds are seeded in a 6 cm diameter plastic cup filled with seedling cultivation soil, kept in a seedling storage at 30 ° C. for 3 days after sowing, and further controlled for 11 days in a 25 ° C. moist chamber. To determine the diseased seedling rate according to Equation 1 and to Equation 2
Was used to calculate the control value. The seeding rate per ward is wet paddy 4
g (approximately 80-90 tablets), the test was performed in triplicate. Table 4 shows the obtained results.

【００４０】[0040]

【表４】 [Table 4]

【００４１】結果は表４に示す通り、ＳＫＴ−１菌、Ｓ
ＫＴ−２菌及びＳＫＴ−３とも、イネもみ枯細菌病に対
して明らかな発病抑制効果を示した。また、ＳＫＴ−１
菌の分生胞子を用いて調製した製剤においても同様の発
病抑制効果が認められた。The results are shown in Table 4, as shown in Table 4.
Both KT-2 bacteria and SKT-3 showed a clear disease-suppressing effect against rice blight bacterial rot. Also, SKT-1
A similar disease-suppressing effect was observed in the preparation prepared using the conidia of the fungus.

【００４２】[0042]

【実施例６】トリコデルマ・アトロビリデＳＫＴ−１菌
株、ＳＫＴ−２菌株及びＳＫＴ−３菌株をＰＤＡ平板培
地上で７〜１４日間培養し、得られた分生胞子を蒸留水
に懸濁し、胞子懸濁液を調製した。この胞子懸濁液或る
いは実施例１で作製したＳＫＴ−１菌株の水和剤を２０
０倍又は２０００倍に希釈した菌液に、イネ苗立枯細菌
病罹病籾（品種黄金晴、開花期に苗立枯細菌病菌を接
種）を、１５℃で５日間浸漬（浴比１：２）した後、菌
液を捨て３２℃の湿室内に１日間保って催芽させた。育
苗培土を充填した径６ｃｍのプラスチックカップに催芽
種子を播種し、播種後３日間、３０℃の育苗庫内に保
ち、更に２５℃の湿室内で７日間管理した後に、全苗に
ついて表２の基準によって発病程度を調査し、数２によ
り発病度を求め、更に無処理区との比から数３により防
除価を算出した。１区当たりの播種量は湿籾４ｇ（約８
０〜９０粒）、試験は３反復で行なった。得られた結果
を表５に示した。Example 6 Trichoderma atroviride SKT-1, SKT-2, and SKT-3 strains were cultured on a PDA plate medium for 7 to 14 days, and the resulting conidia were suspended in distilled water and suspended in spore suspension. A suspension was prepared. The spore suspension or the wettable powder of the SKT-1 strain prepared in Example 1 was used for 20 times.
Rice seedlings infected with bacterial seedling blight (variety Koganeharu, inoculated with bacterial seedling blight at flowering stage) are immersed in a bacterial solution diluted 0 or 2000 times at 15 ° C. for 5 days (bath ratio 1: 2). ), The bacterial solution was discarded, and the cells were kept in a humid chamber at 32 ° C for 1 day for germination. Seedlings are seeded in a plastic cup having a diameter of 6 cm filled with the seedling cultivation soil, kept in a seedling storage at 30 ° C. for 3 days after sowing, and further controlled in a humidity chamber at 25 ° C. for 7 days. The degree of onset was investigated based on the standard, the degree of onset was determined by Equation 2, and the control value was calculated by Equation 3 from the ratio with the untreated plot. The seeding rate per ward is 4 g of wet paddy (about 8
0-90) and the test was performed in triplicate. Table 5 shows the obtained results.

【００４３】[0043]

【表５】 [Table 5]

【００４４】結果は表５に示す通り、ＳＫＴ−１菌、Ｓ
ＫＴ−２菌及びＳＫＴ−３菌とも、イネ苗立枯細菌病に
対して明らかな発病抑制効果を示した。また、ＳＫＴ−
１菌の分生胞子を用いて調製した製剤においても同様の
発病抑制効果が認められた。The results are shown in Table 5, as shown in Table 5.
Both KT-2 bacteria and SKT-3 bacteria showed a clear disease-suppressing effect against bacterial seedling blight disease. Also, SKT-
A similar disease-suppressing effect was observed in a preparation prepared using a conidia of one bacterium.

【００４５】[0045]

【実施例７】ＰＤ液体培地で２７℃、５２時間振とう培
養により得られたイネ褐条病菌（Pseudomonas avenae）
懸濁液（１０8ｃｆｕ／ｍｌ）にイネ種籾（品種黄金
晴）を減圧条件下で１時間浸漬接種し、イネ褐条病罹病
籾を作成した。トリコデルマ・アトロビリデＳＫＴ−１
菌株、ＳＫＴ−２菌株及びＳＫＴ−３菌株をＰＤＡ平板
培地上で７〜１４日間培養し、得られた分生胞子を蒸留
水に懸濁して得た胞子懸濁液或るいは実施例１で作製し
たＳＫＴ−１菌株の水和剤を２００倍又は２０００倍に
希釈した菌液に、上記イネ褐条病罹病籾を、１５℃で５
日間浸漬（浴比１：２）した後、菌液を捨て３２℃の湿
室内に１日間保って催芽させた。育苗培土を充填した径
６ｃｍのプラスチックカップに催芽種子を播種し、播種
後３日間、３０℃の育苗庫内に保ち、更に２５℃の湿室
内で１２日間管理した後に、全苗について発病の有無を
調査し数１により発病苗率を、また数２により防除価を
算出した。１区当たりの播種量は湿籾４ｇ（約８０〜９
０粒）、試験は３反復で行なった。得られた結果を表６
に示した。[Example 7] Rice brown streak (Pseudomonas avenae) obtained by shaking culture in a PD liquid medium at 27 ° C for 52 hours.
Rice seed rice (cultivar Koganebare) was immersed and inoculated in the suspension (10 8 cfu / ml) under reduced pressure for 1 hour to prepare rice brown streak-affected rice. Trichoderma atroviride SKT-1
The strain, the SKT-2 strain and the SKT-3 strain were cultured on a PDA plate medium for 7 to 14 days, and the conidia obtained were suspended in distilled water. To a bacterial solution prepared by diluting the prepared wettable powder of the SKT-1 strain 200-fold or 2000-fold, the rice paddy diseased with rice streak was added at 15 ° C for 5 times.
After immersion for one day (bath ratio 1: 2), the bacterial solution was discarded and kept in a moist room at 32 ° C. for one day to cause germination. Seeding seeds are seeded in a 6 cm diameter plastic cup filled with seedling cultivation soil, kept in a nursery room at 30 ° C. for 3 days after sowing, and further controlled for 12 days in a humidity chamber at 25 ° C., after which all the seedlings are affected. And the disease-causing seedling rate was calculated from Equation 1 and the control value was calculated from Equation 2. The seeding amount per ward is 4 g of wet paddy (about 80-9
0), and the test was performed in triplicate. Table 6 shows the obtained results.
It was shown to.

【００４６】[0046]

【表６】 [Table 6]

【００４７】結果は表６に示す通り、ＳＫＴ−１菌、Ｓ
ＫＴ−２菌及びＳＫＴ−３菌とも、イネ褐条病に対して
明らかな発病抑制効果を示した。また、ＳＫＴ−１菌の
分生胞子を用いて調整した製剤においても同様の発病抑
制効果が認められた。As shown in Table 6, the results were as follows.
Both KT-2 bacteria and SKT-3 bacteria showed a clear disease-suppressing effect on rice brown streak. In addition, a similar disease-suppressing effect was observed in a preparation prepared using conidia of SKT-1 bacteria.

【００４８】[0048]

【発明の効果】イネの育苗時に発生する病害のうち、イ
ネ苗立枯細菌病、イネもみ枯細菌病、イネ褐条病等の各
種細菌に起因する細菌病害は、特に防除困難な病害であ
って、種子の段階で防除する必要があり、作物の段階で
は防除することができない厄介な病害である。しかも従
来から用いられている種子消毒剤で比較的有効なものは
わずかであり、しかも有効性のみについてみても充分な
ものとはいえない。EFFECTS OF THE INVENTION Among the diseases that occur during rice seedling raising, bacterial diseases caused by various bacteria such as rice seedling blight, rice wilt, and rice streak are particularly difficult to control. It is a troublesome disease that needs to be controlled at the seed stage and cannot be controlled at the crop stage. Moreover, only a relatively small number of conventionally used seed disinfectants are relatively effective, and the effectiveness alone is not sufficient.

【００４９】更に、これら化学殺菌剤の使用は、耐性菌
の出現を招来するだけでなく、使い残しの殺菌剤、散布
器具や容器洗浄後の殺菌剤含有廃液の処理が大きな問題
としてクローズアップされてきており、土壌汚染、河川
汚染を防止する必要上、該廃液を容易に廃棄できなくな
ってきている。Furthermore, the use of these chemical disinfectants not only leads to the emergence of resistant bacteria, but also disposes of residual disinfectants, disinfectants and waste liquids containing disinfectants after washing containers and containers as major problems. The waste liquid cannot be easily disposed of because it is necessary to prevent soil contamination and river contamination.

【００５０】これらの問題点を一挙に解決するのに成功
したのが本発明であって、化学合成殺菌剤を使用すると
いう従来の発想を転換して生物農薬に着目し、鋭意研究
の結果、イネに病原性のないトリコデルマ属菌が、イネ
の細菌性病害の防除に有効であり、イネ種子に適用して
も種子に害を与えることがなく、更に、土壌や水中にお
いてもその有効性が低下することがない点を確認し、こ
こにはじめて上記した問題点のないすぐれたイネの種子
伝染性細菌病防除剤の完成に至ったものである。The present invention has succeeded in solving these problems all at once. The present inventors have changed the conventional idea of using a chemically synthesized fungicide and focused on biological pesticides. Trichoderma spp., Which is not pathogenic to rice, is effective in controlling bacterial diseases of rice, does not harm the seeds even when applied to rice seeds, and is also effective in soil and water. It was confirmed that the agent did not decrease, and it was for the first time that an excellent rice seed infectious bacterial disease control agent free from the above-mentioned problems was completed.

【００５１】また本発明に係る防除剤は、環境が変化し
てもその有効性が変化することがないので、イネ種子を
直接処理できることはもちろんのこと、イネ種子の播種
前、播種時、又は播種後の少なくともひとつの時期に、
イネの育苗培体（土壌、砂、ロックウール等）に潅注、
添加、混和処理することにより、イネの育苗時に発生す
る細菌性病害を防除することができる。Further, the controlling agent according to the present invention does not change its effectiveness even if the environment changes, so that it is possible to directly treat rice seeds, and also before or during sowing of rice seeds, or At least one time after sowing,
Irrigation of rice seedling culture medium (soil, sand, rock wool, etc.)
By adding and mixing, it is possible to control bacterial diseases that occur during rice seedling raising.

【００５２】更にまた、本発明で使用するトリコデルマ
属菌は、イネの育苗時に発生する細菌性病害に対する防
除スペクトラムが広いため、各病原菌を同時に防除する
ことができ、一層の省力化、省資源化、環境保全が促進
される。Furthermore, the Trichoderma genus used in the present invention has a broad spectrum of control against bacterial diseases that occur during rice seedling raising, so that each pathogenic bacterium can be simultaneously controlled, further saving labor and resources. , Environmental conservation is promoted.

───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 ＦＩ Ｃ１２Ｒ 1:885） (72)発明者 市川 健 静岡県藤枝市兵太夫602−９ (72)発明者 伊代住 浩幸 静岡県磐田郡竜洋町岡288−１ (72)発明者 牧野 孝宏 静岡県浜松市庄和町1798──────────────────────────────────────────────────の Continuation of the front page (51) Int.Cl. 6 Identification code FI C12R 1: 885) (72) Inventor Takeshi Ken Ichikawa 602-9 Hyota, Fujieda-shi, Shizuoka Prefecture (72) Inventor Hiroyuki Iyosumi Iwata-gun, Shizuoka Prefecture 288-1 Ryuyocho Oka (72) Inventor Takahiro Makino 1798 Showacho, Hamamatsu City, Shizuoka Prefecture

Claims (9)

【特許請求の範囲】[Claims] 【請求項１】 トリコデルマ（Trichoderma）属に属
し、イネの育苗時に発生する細菌性病害に対して防除能
を有する、下記に示す微生物の中から選ばれる少なくと
もひとつを有効成分とするイネの種子伝染性細菌病防除
剤。トリコデルマ・アトロビリデ(Trichoderma atrovir
ide)、トリコデルマ・アウレオビリデ(Trichoderma aur
eoviride)、トリコデルマ・ビリデ(Trichoderma virid
e)、トリコデルマ・ハルジアナム(Trichoderma haruzia
num)、トリコデルマ・ロンギブラキアタム（Trichoderm
a longibrachiatum)、トリコデルマ・シュードコニンギ
(Trichoderma pseudokoningii)、トリコデルマ・ポリス
ポラム(Trichoderma polysporum)、トルコデルマ・ハマ
タム(Trichoderma hamatum)。1. A seed transmission of rice comprising at least one of the following microorganisms as an active ingredient, which belongs to the genus Trichoderma and has an ability to control bacterial diseases that occur during seedling raising of rice. Bacterium disease control agent. Trichoderma atrovir
ide), Trichoderma aurobiride
eoviride), Trichoderma virid
e), Trichoderma haruzia
num), Trichoderm longibrachiatum
a longibrachiatum), Trichoderma pseudoconing
(Trichoderma pseudokoningii), Trichoderma polysporum, and Trichoderma hamatum. 【請求項２】 トリコデルマ属（Trichoderma）属に属
し、イネの育苗時に発生する細菌性病害に対して防除能
を有する微生物が、トリコデルマ・アトロビリデ(Trich
oderma atroviride) SKT-1株(FERM P-16510)、トリコデ
ルマ・アトロビリデ(Trichoderma atroviride) SKT-2株
(FERM P-16511)、トリコデルマ・アトロビリデ(Trichod
erma atroviride) SKT-3株(FERM P-17021)よりなる群の
中から選ばれる少なくとも１菌株であることを特徴とす
る請求項１に記載のイネの種子伝染性細菌病害防除剤。2. A microorganism belonging to the genus Trichoderma and capable of controlling bacterial diseases that occur during rice seedling raising, is Trichoderma atroviride (Trich).
oderma atroviride) SKT-1 strain (FERM P-16510), Trichoderma atroviride SKT-2 strain
(FERM P-16511), Trichodema Atroviride (Trichod
2. The agent for controlling bacterial infectious diseases of rice seeds according to claim 1, which is at least one strain selected from the group consisting of erma atroviride) SKT-3 strain (FERM P-17021). 【請求項３】 イネの育苗時に発生する細菌性病害が、
イネ苗立枯細菌病、イネもみ枯細菌病及びイネ褐条病か
ら選ばれる少なくともひとつであること、を特徴とする
請求項１又は２に記載のイネの種子伝染性細菌病防除
剤。3. The bacterial disease occurring during rice seedling raising,
The agent for controlling rice seed-borne infectious disease according to claim 1 or 2, wherein the agent is at least one selected from rice seedling blight, rice blight, and rice brown streak. 【請求項４】 請求項１〜３の少なくとも何れか１項に
記載のイネの種子伝染性細菌病防除剤で処理してなる病
害防除性イネ種子。4. A disease-controlling rice seed which is treated with the rice seed-transmitting bacterial disease controlling agent according to at least one of claims 1 to 3. 【請求項５】 請求項１〜３の少なくとも何れか１項に
記載のイネの種子伝染性細菌病防除剤で処理してなる病
害防除性イネ育苗培体。5. A disease-controlling rice seedling culture obtained by treating with the rice seed-infectious bacterial disease controlling agent according to at least one of claims 1 to 3. 【請求項６】 請求項１〜３の少なくとも何れか１項に
記載のイネの種子伝染性細菌病防除剤で処理してなる病
害防除性イネ種子及び／又はイネ育苗培体を含有するこ
と、を特徴とするイネ育苗容器。6. A disease-controlling rice seed and / or a rice seedling culture obtained by treating with the rice seed infectious bacterial disease controlling agent according to at least one of claims 1 to 3. A rice seedling raising container characterized by the following. 【請求項７】 請求項１〜３の少なくとも何れか１項に
記載のイネの種子伝染性細菌病防除剤をイネの種子に浸
種前、浸種時又は浸種後に浸漬、噴霧、塗布又は粉衣処
理することを特徴とするイネの育苗時に発生する細菌性
病害防除方法。7. The rice seed infectious bacterial disease controlling agent according to at least any one of claims 1 to 3, before, during or after soaking in rice seeds, dipping, spraying, coating or dressing. A method for controlling bacterial diseases occurring during rice seedling raising. 【請求項８】 請求項１〜３の少なくとも何れか１項に
記載のイネの種子伝染性細菌病防除剤をイネの種子の播
種前、播種時又は播種後にイネの育苗培体に潅注又は混
和処理すること、を特徴とするイネの育苗時に発生する
細菌性病害防除方法。8. The rice seed infectious bacterial disease controlling agent according to at least any one of claims 1 to 3 is irrigated or mixed with a rice seedling culture medium before, during or after sowing of rice seeds. A method for controlling bacterial diseases occurring during rice seedling raising, comprising treating. 【請求項９】 トリコデルマ・アトロビリデ(Trichoder
ma atroviride) SKT-1株(FERM P-16510)又はトリコデル
マ・アトロビリデ(Trichoderma atroviride)SKT-2株(FE
RM P-16511)又はトリコデルマ・アトロビリデ(Trichode
rma atroviride) SKT-3株(FERM P-17021)。9. Trichoderma atroviride (Trichoder)
ma atroviride) SKT-1 strain (FERM P-16510) or Trichoderma atroviride) SKT-2 strain (FE
RM P-16511) or Trichoderma atroviride (Trichode
rma atroviride) SKT-3 strain (FERM P-17021).
JP33650098A 1997-11-13 1998-11-12 Disease control agent for rice seedling Expired - Lifetime JP3691265B2 (en)

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