JP2021533765A - Pichia kluyveriを用いたアルコールフリーの発酵野菜ジュースの製造 - Google Patents
Pichia kluyveriを用いたアルコールフリーの発酵野菜ジュースの製造 Download PDFInfo
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Abstract
Description
本明細書で使用される場合、用語「野菜」とは、ヒトにより消費される植物の食用部分を指す。植物の食用部分は、以下であり得る:根、例えばルタバガ、ビート、ニンジン、サツマイモ;塊茎または貯蔵茎、例えばジャガイモ及びサトイモ;アスパラガス及びコールラビのような茎;芽、例えばキャベツのつぼみ;球根、例えばタマネギ及びニンニク;葉柄、例えばセロリ及びルバーブ;葉、例えばキャベツ、レタス、パセリ、及びほうれん草;未熟な花、例えばカリフラワー、ブロッコリー、及びアーティチョーク。
a)根菜を含む基質を提供し、
b)少なくとも1つのPichia kluyveri株を、前記基質に添加し、そして
c)Pichia kluyveri株を含む基質を発酵させることを含む。
a)根菜を含む基質を提供し、
b)基質のpHを調節し、
c)少なくとも1つのPichia kluyveri株を、前記基質に添加し、そして
d)Pichia kluyveri株を含む基質を発酵させることを含む。
a)根菜を含む基質を提供し、
b)基質のpHを調節し、
c)少なくとも1つのPichia kluyveri株を、前記基質に添加し、そして
d)Pichia kluyveri株を含む基質を発酵させることを含む方法を提供する。
以下の項目は、本発明の好ましい実施形態である:
1. a)根菜を含む基質を提供する工程、
b)少なくとも1つのPichia kluyveri株を、前記基質に添加する工程、
c)Pichia kluyveri株を含む基質を48時間未満、発酵させる工程、
d)発酵された野菜ジュースを入手する工程、を含んで成る、野菜ジュースの土のような異臭を低める方法。
2.前記根菜が、ニンジン、ビーツ、パースニップ、スウェード、ウコン、生姜、ジャガイモ、サツマイモ、カブ、ルタバガ、ユカ、コールラビ、タマネギ、ニンニク、セロリの根、セイヨウワサビ、大根、ヒカマ、ラディッシュ、キクイモ及び山芋から成る群から選択される、項目1に記載の方法。
3.前記Pichia kluyveriが、下記群:
a)German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; DSMZ), Inhoffenstr. 7B, D-38124 Braunschweigで、2014年3月5日に寄託され、そして受託番号DSM28484を与えられたPichia kluyveri、
b)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02271を与えられたPichia kluyveri、
c)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02272を与えられたPichia kluyveri、及び
d)a)、b)又はc)の変異体、
から成る群から選択される、項目1に記載の方法。
4.酢酸イソアミルと酢酸エチルの重量比が少なくとも0.1、好ましくは少なくとも0.2である、項目1〜3のいずれか1項に記載の方法。
5.工程b)がさらに、基質に乳酸菌株を添加することを含む、項目1〜4のいずれか1項に記載の方法。
6.工程c)における発酵が、4〜35℃、より好ましくは、18〜22℃の温度で行われる、項目1〜5のいずれか1項に記載の方法。
7.前記発酵野菜ジュース中のβ−フェニルエタノールが少なくとも0.4ppmである、項目1〜6のいずれか1項に記載の方法。
8.前記発酵野菜ジュース中のα−テルピネオールが少なくとも50ppbである、項目1〜7のいずれか1項に記載の方法。
9.前記発酵野菜ジュース中のc-ウイスキーラクトン又はバニリンが少なくとも4ppbである、項目1〜8のいずれか1項に記載の方法。
10.前記発酵野菜ジュース中のバニリンが少なくとも4ppbである、項目1〜8のいずれか1項に記載の方法。
11.項目1〜10のいずれか1項記載の方法により得られた発酵野菜ジュース。
12.根菜及びPichia kluyveriを含む発酵野菜ジュース
13.請求項12
前記Pichia kluyveriが、下記群:
a)German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; DSMZ), Inhoffenstr. 7B, D-38124 Braunschweigで、2014年3月5日に寄託され、そして受託番号DSM28484を与えられたPichia kluyveri、
b)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02271を与えられたPichia kluyveri、
c)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02272を与えられたPichia kluyveri、及び
d)a)、b)又はc)の変異体、
から成る群から選択される、項目12に記載の発行野菜ジュース。
14.酢酸エチルに対する酢酸イソアミルの重量比が、少なくとも0.1、好ましくは0.2であり、そして/又はエタノールに対する酢酸イソアミルの重量比は、少なくとも0.002、好ましくは少なくとも0.0008である、項目12又は13に記載の発酵野菜ジュース。
15.発酵野菜ジュース中のβ−フェニルエタノールが、少なくとも0.4ppmである、項目12〜14のいずれか1項記載の発酵野菜ジュース。
16.発酵野菜ジュース中のα−テルピネオールは、少なくとも50ppbである、項目12〜15のいずれか1項記載の発酵野菜ジュース。
17.発酵野菜ジュース中のc−ウィスキーラクトンは、少なくとも4ppbである、項目12〜16のいずれか1項記載の発酵野菜ジュース。
18.発酵野菜ジュース中のc−バニリンは、少なくとも4ppbである、項目12〜17のいずれか1項記載の発酵野菜ジュース。
19.根菜を発酵するためへのPichia kluyveriの使用。
20.前記根菜が、ニンジン、ビーツ、パースニップ、スウェード、ウコン、生姜、ジャガイモ、サツマイモ、カブ、ルタバガ、ユカ、コールラビ、タマネギ、ニンニク、セロリの根、セイヨウワサビ、大根、ヒカマ、ラディッシュ、キクイモ及び山芋から成る群から選択される。項目19記載の使用。
21.前記Pichia kluyveriが、下記群:
a)German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; DSMZ), Inhoffenstr. 7B, D-38124 Braunschweigで、2014年3月5日に寄託され、そして受託番号DSM28484を与えられたPichia kluyveri、
b)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02271を与えられたPichia kluyveri、
c)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02272を与えられたPichia kluyveri、及び
d)a)、b)又はc)の変異体、
から成る群から選択される、項目19又は20に記載の使用。
22.German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; DSMZ), Inhoffenstr. 7B, D-38124 Braunschweigで、2014年3月5日に寄託され、そして受託番号DSM28484を与えられたPichia kluyveri、及びその変異体。
23.項目22記載のPichia kluyveriを含む発酵のための組成物。
24.前記組成物が、凍結、凍結乾燥又は液体形で存在する、項目23に記載の組成物。
出願人は、特許が付与される日まで、下記の寄託微生物のサンプルが専門家のみが利用できるようにすることを要求する。
4.5のpHのニンジンジュースを、野菜の基質として提供した。それを、ニンジンジュース濃縮液を希釈することにより調製した。
エタノールを、Boehringer MannheimのEthanol Enzymatic Bioanalysis キットにより測定した。Pichia kluyveri株1及び株2による24時間の発酵の後、発酵生成物のエタノール濃度を測定した。表1は、未発酵及び発酵ニンジンジュース中のエタノールの結果を示す。
全ての発酵製品のフレーバー分析を、スペインのZaragozaにあるFlavor Analysis and Ecokogyの実験室で行った。表2〜6は、サンプル中の異なるフレーバー化合物の濃度を示す。
新鮮な酸性化されていないビート根ジュースを、野菜基質として提供した。それを、ビート根を圧縮することにより調製した。実験室規模の発酵(400ml)を行った。ビート根ジュースを、実施例1に記載のように、同じPichia kluyveri、すなわちPichia kluyveri株1及びPichia kluyveri株2により接種した。発酵を、20℃で24時間、インキュベーターに配置した。その後、サンプルを採取した。
Lawless et al (Lawless, H. T., and H. Heymann. "Sensory Evaluation of Food: Practices and Principals." Food Science Texts Series. Chapman and Hall. New York (2010))による記述的分析技法を用いての感覚的評価を、Pichia kluyveriがフレーバープロファイルにいかに寄与するかを評価するために実施した。
Claims (15)
- 野菜ジュースの土のような異臭を低める方法であって、以下の工程
a)根菜を含む基質を提供する工程、
b)少なくとも1つのPichia kluyveri株を、前記基質に添加する工程、
c)Pichia kluyveri株を含む基質を発酵させる工程、
d)発酵された野菜ジュースを入手する工程、
を含んで成る方法。 - 前記根菜が、ニンジン、ビーツ、パースニップ、スウェード、ウコン、生姜、ジャガイモ、サツマイモ、カブ、ルタバガ、ユカ、コールラビ、タマネギ、ニンニク、セロリの根、セイヨウワサビ、大根、ヒカマ、ラディッシュ、キクイモ及び山芋から成る群から選択される、請求項1に記載の方法。
- 前記Pichia kluyveriが、下記群:
a)German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; DSMZ), Inhoffenstr. 7B, D-38124 Braunschweigで、2014年3月5日に寄託され、そして受託番号DSM28484を与えられたPichia kluyveri、
b)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02271を与えられたPichia kluyveri、
c)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02272を与えられたPichia kluyveri、及び
d)a)、b)又はc)の変異体、
から成る群から選択される、請求項1又は2に記載の方法。 - 酢酸イソアミルと酢酸エチルの重量比が少なくとも0.1、好ましくは少なくとも0.2であり、そして/又は酢酸イソアミルとエタノールの重量比が少なくとも0.0002、好ましくは少なくとも0.0008である、請求項1〜3のいずれか1項に記載の方法。
- 工程b)がさらに、基質に乳酸菌株を添加することを含む、請求項1〜4のいずれか1項に記載の方法。
- 工程c)における発酵が、4〜35℃、より好ましくは、18〜22℃の温度で行われる、請求項1〜5のいずれか1項に記載の方法。
- 前記発酵野菜ジュース中のβ−フェニルエタノールが少なくとも0.4ppmである、請求項1〜6のいずれか1項に記載の方法。
- 前記発酵野菜ジュース中のα−テルピネオールが少なくとも50ppbである、請求項1〜7のいずれか1項に記載の方法。
- 前記発酵野菜ジュース中のc-ウイスキーラクトン又はバニリンが少なくとも4ppbである、請求項1〜8のいずれか1項に記載の方法。
- 前記ジュースがPichia kluyveriを含む、請求項1〜9のいずれか1項に記載の方法により得られた発酵野菜ジュース。
- 根菜及びPichia kluyveriを含む発酵野菜ジュース。
- 前記Pichia kluyveriが、下記群:
a)German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; DSMZ), Inhoffenstr. 7B, D-38124 Braunschweigで、2014年3月5日に寄託され、そして受託番号DSM28484を与えられたPichia kluyveri、
b)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02271を与えられたPichia kluyveri、
c)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02272を与えられたPichia kluyveri、及び
d)a)、b)又はc)の変異体、
から成る群から選択される、請求項11に記載の発行野菜ジュース。 - 根菜を発酵するためへのPichia kluyveriの使用。
- 前記Pichia kluyveriが、下記群:
a)German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; DSMZ), Inhoffenstr. 7B, D-38124 Braunschweigで、2014年3月5日に寄託され、そして受託番号DSM28484を与えられたPichia kluyveri、
b)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02271を与えられたPichia kluyveri、
c)University of Auckland, School of Biological Sciences, Auckland 1142, New Zealand によりthe National Measurement Institute, 541-65 Clarke Street, South Melbourne, Victoria 3205, Australiaで、2006年8月24日に寄託され、そして受託番号V06/02272を与えられたPichia kluyveri、及び
d)a)、b)又はc)の変異体、
から成る群から選択される、請求項13に記載の使用。 - German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; DSMZ), Inhoffenstr. 7B, D-38124 Braunschweigで、2014年3月5日に寄託され、そして受託番号DSM28484を与えられたPichia kluyveri、及びその変異体。
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