JP2019050744A - Ganoderma lucidum mycelium culture method at soybean medium and ganoderma lucidum mycelium health food product including soybean - Google Patents
Ganoderma lucidum mycelium culture method at soybean medium and ganoderma lucidum mycelium health food product including soybean Download PDFInfo
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- 244000068988 Glycine max Species 0.000 title claims abstract description 69
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 17
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 17
- 235000013402 health food Nutrition 0.000 title claims abstract description 17
- 238000012136 culture method Methods 0.000 title claims abstract description 7
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- 238000011081 inoculation Methods 0.000 claims description 12
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- 235000013305 food Nutrition 0.000 abstract description 4
- 238000001035 drying Methods 0.000 abstract 1
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- 239000007787 solid Substances 0.000 description 7
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- 239000001963 growth medium Substances 0.000 description 4
- 230000018044 dehydration Effects 0.000 description 3
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- 238000001816 cooling Methods 0.000 description 2
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Abstract
Description
本発明は大豆培地を用いた霊芝菌糸体の培養方法と、培養済みの霊芝菌糸体とその培養に用いた大豆を含む健康食品に関する。 The present invention relates to a method for cultivating Ganoderma mycelium using a soybean medium, a cultured Ganoderma mycelium, and a health food containing soybean used for the cultivation.
大豆を固形培地とした霊芝菌糸体の培養方法はこれまでも開発されている（特許文献１）。 A method for culturing Ganoderma mycelium using soybean as a solid medium has been developed (Patent Document 1).
特許文献１の培養方法は、大豆及び胡麻を固体培地（固形培地）とし、それに霊芝種菌を接種する方法である。この出願当時の霊芝種菌は固形種（こけいたね）であったため、固形種が固形培地全般に行き渡りにくい、接種しにくい、接種量がばらつき易いという難点がある。また、接種後の霊芝菌糸体の増殖率が低い、ばらつきなく接種するためには熟練を要するといった難点があった。 The culture method of Patent Document 1 is a method in which soybean and sesame are used as a solid medium (solid medium) and inoculated with Ganoderma spp. Since the Ganoderma spp. At the time of this application was a solid species (Kokeitane), the solid species are difficult to spread over the whole solid medium, difficult to inoculate, and the inoculation amount tends to vary. In addition, the growth rate of Ganoderma mycelium after inoculation is low, and there is a problem that skill is required to inoculate without variation.
本発明の課題は、霊芝菌糸体の増殖率の向上、接種作業の容易化、接種量の均等化等を実現することにある。 An object of the present invention is to improve the growth rate of Ganoderma mycelium, facilitate inoculation work, equalize the inoculation amount, and the like.
本発明の大豆培地での霊芝菌体糸培養方法は、大豆を液体に浸漬し、その大豆を大豆培地として培養容器に入れ、培養容器内の大豆培地に霊芝菌糸体の液種（えきたね）を接種し、接種後の培養容器を培養室内において、霊芝菌糸体を培養する方法である。液種は霊芝菌糸体を液体内に分散させたものである。液種の接種は一度の噴射量が均一又はほぼ均一である噴射式の接種機で、個々の培養容器内に噴射して、各培養容器内の大豆培地に均等量ずつ接種して、接種量にばらつきがなく、個々の培養容器内の増殖量がほぼ均等になるようにする。 The method for cultivating ganoderma mycelium in a soybean medium of the present invention comprises immersing soybeans in a liquid, placing the soybeans in a culture container as a soybean medium, and adding a liquid species of ganoderma mycelium to the soybean medium in the culture container. This is a method for cultivating Reishi mycelium in a culture chamber after inoculation. The liquid type is obtained by dispersing Ganoderma mycelium in the liquid. Inoculation of the liquid type is a spray type inoculator with a uniform or almost uniform injection amount, spraying into individual culture containers, inoculating an equal amount of soybean medium in each culture container, and inoculating amount So that the amount of growth in each culture vessel is almost uniform.
本発明の大豆を含む霊芝菌糸体健康食品は、培養された霊芝菌糸体と培養後の大豆培地をフリ−ズドライし、粉砕して粉末化（微粉末化を含む）するとか、顆粒状、錠剤、カプセル、液状といった所望形態にしたものである。 The Ganoderma mycelium health food containing soybean of the present invention is obtained by freeze-drying the cultured ganoderma mycelium and the cultured soybean medium, pulverizing it into a powder (including fine powder), or granular form , Tablets, capsules and liquid forms.
本発明の大豆培地での霊芝菌糸体培養方法は次のような効果がある。
（１）大豆培地に霊芝菌糸体の液種を接種するので、培養容器内の大豆培地の上側だけでなく内部及び下側までほぼ均等に接種でき、増殖率が向上する。
（２）接種機で一定量ずつ噴射して接種することにより、接種量のばらつきがほとんどなく、熟練者でなくても均等に接種できる。
The method for cultivating ganoderma mycelium in the soybean medium of the present invention has the following effects.
(1) Since the liquid species of Ganoderma mycelium is inoculated into the soybean medium, it can be inoculated almost uniformly not only on the upper side of the soybean medium in the culture container but also on the inside and the lower side, and the growth rate is improved.
(2) By injecting by inoculating a certain amount with an inoculator, there is almost no variation in the inoculation amount, and even an unskilled person can inoculate evenly.
本発明の大豆を含む霊芝菌糸体健康食品は次のような効果がある。
（１）霊芝はβグルカン、アミノ酸群等の有効成分が豊富であることが知られている。本発明の大豆を含む霊芝菌糸体健康食品には、これら有効成分をはじめとして、各種抗癌成分が含まれており、多数の機能性健康増進物質の加水分解酵素も含まれているので、医薬品や健康食品等の各分野に著しく有用である。
（２）霊芝菌糸体のみならず、その培養に用いた大豆をもフリーズドライして、服用するのに適した所望形態にしてあるため、服用し易い。
（３）霊芝菌糸体だけでなく、培地として使用された大豆をも含むため、大豆の成分、養分も含まれたものとなる。
The Ganoderma mycelium health food containing soybean of the present invention has the following effects.
(1) Ganoderma is known to be rich in active ingredients such as β-glucan and amino acid groups. Ganoderma mycelium health food containing soybean of the present invention contains various anticancer ingredients including these active ingredients, and also contains hydrolytic enzymes of many functional health promoting substances, It is extremely useful in various fields such as pharmaceuticals and health foods.
(2) Since not only the reishi mycelium but also the soybeans used for its cultivation are freeze-dried and made into a desired form suitable for taking, it is easy to take.
(3) Since not only the reishi mycelium but also the soybean used as a culture medium, the soybean component and nutrients are included.
本発明の実施形態を以下に説明する。その実施形態は一例である。本発明は以下の実施形態に限定されるのではない。 Embodiments of the present invention will be described below. The embodiment is an example. The present invention is not limited to the following embodiments.
（大豆培地での霊芝菌糸体培養方法の実施形態）
この実施形態は大豆のみを培地とする場合であるが、本発明では大豆の他に、必要に応じての他の食品、例えば、胡麻、玄米、麦等の穀類を混合して培地とすることもできる。大豆の種類、他の穀類は人が食用可能な物であれば特に制約はない。混合割合は任意に選択することができる。
(Embodiment of Ganoderma mycelium culture method in soybean medium)
This embodiment is a case where only soybean is used as a medium, but in the present invention, in addition to soybeans, other foods as required, for example, cereals such as sesame, brown rice and wheat are mixed to form a medium. You can also. The type of soybean and other grains are not particularly limited as long as they are human edible. The mixing ratio can be arbitrarily selected.
［培養工程］
１．浸漬工程
固形培地の原料である大豆を水道水、純水、その他、培養に好ましい補助液を含む液体に浸漬する。大豆の量は必要に応じて決定する。浸漬時間は１２〜２４時間程度とする。
[Culture process]
1. Immersion step Soybean, which is a raw material of the solid medium, is immersed in a liquid containing tap water, pure water, and other auxiliary liquids preferable for culture. The amount of soy is determined as needed. The immersion time is about 12 to 24 hours.
２．脱水工程
浸漬した大豆を液体から取り出し脱水する。脱水は遠心分離機、その他の機械で行うことができる。脱水後の水分は４０〜８０％程度が好ましい。
2. Dehydration process The soaked soybeans are removed from the liquid and dehydrated. Dehydration can be performed with a centrifuge or other machines. The water after dehydration is preferably about 40 to 80%.
３．培養容器への収容工程
脱水した大豆（大豆培地）を培養容器に入れる。培養容器は耐熱性、耐圧性があり、蓋のある樹脂製のボトル、ガラス製のビン等のガスバリア性のあるものが適する。軽量なものが好ましい。形状は大豆を入れ易いものが良く、容量は入れる大豆の量に合わせて選択する。大豆を詰めた後は蓋を閉める。
3. Step of storing in culture container Dehydrated soybean (soybean medium) is placed in the culture container. The culture vessel has heat resistance and pressure resistance, and a gas barrier such as a resin bottle with a lid or a glass bottle is suitable. A lightweight one is preferred. The shape should be easy to put soy, and the capacity should be selected according to the amount of soy. Close the lid after filling with soybeans.
４．滅菌工程
大豆培地を収容した培養容器を滅菌釜（高圧蒸気滅菌器：オートクレーブ）に入れて、大豆培地を培養容器ごと滅菌する。滅菌温度は１２１℃程度、滅菌時間は１０分程度が適する。滅菌釜には汎用の滅菌釜を使用することができる。霊芝菌糸体接種工程で使用する接種機、その他無菌条件下で使用する器具も滅菌釜で滅菌する。滅菌には滅菌釜以外のものも使用可能である。
4). Sterilization process The culture container containing the soybean culture medium is placed in a sterilization kettle (high-pressure steam sterilizer: autoclave), and the soybean culture medium is sterilized together with the culture container. A sterilization temperature of about 121 ° C. and a sterilization time of about 10 minutes are suitable. A general-purpose sterilization pot can be used as the sterilization pot. Sterilize the inoculator used in the reishi mushroom mycelia inoculation process and other instruments used under aseptic conditions in a sterile kettle. Other than the sterilization pot can be used for sterilization.
５．自然冷却工程
滅菌工程で加熱された培養容器を滅菌釜から取り出して自然冷却させる。
5. Natural cooling process The culture container heated in the sterilization process is taken out of the sterilization pot and allowed to cool naturally.
６．霊芝菌糸体接種工程
自然冷却後の培養容器内の大豆培地に霊芝菌糸体を接種する。霊芝菌糸体は液状の菌（液種）を使用する。接種は噴射式の接種機で行う。接種はクリーンベンチ、その他の無菌室内で行う。接種機は足踏み式のものが適する。一回の足踏みで所定量が噴射されるものが適する。一回の噴射量は調節することができる。液種接種後は培養容器に蓋をして、雑菌の混入を防ぐ。
6). Ganoderma mycelium inoculation process Ganoderma mycelium is inoculated into the soybean medium in the culture container after natural cooling. Ganoderma mycelium uses liquid bacteria (liquid species). Inoculation is done with a jet-type inoculator. Inoculate in a clean bench or other sterile room. A stepping type inoculator is suitable. The one in which a predetermined amount is injected by one step is suitable. A single injection amount can be adjusted. After inoculation, cover the culture container to prevent contamination.
７．培養工程
前記培養容器を培養室に入れて培養する。培養温度（室温）は２５〜３０℃、培養期間は３〜４週間程度、暗黒下が適する。
7). Culture process The culture vessel is placed in a culture chamber and cultured. The culture temperature (room temperature) is 25 to 30 ° C., the culture period is about 3 to 4 weeks, and darkness is suitable.
［大豆を含む霊芝菌糸体健康食品の製造工程］
大豆を含む霊芝菌糸体健康食品を製造するには、次のようにする。
１．トレーへの移し替え
前記１〜７の工程を経て培養された霊芝菌糸体及び培養済みの大豆培地を培養容器から取り出して、フリ−ズドライ用のトレーに敷き詰める（移し替える）。フリ−ズドライ用のトレーのサイズは各種あるが、必要に応じて選択使用することができる。
[Manufacturing process of Ganoderma mycelium health food containing soybeans]
To manufacture Ganoderma mycelium health food containing soy, do as follows.
1. Transfer to Tray Take out the Reishi mushroom mycelium and the cultured soybean medium cultured through the steps 1 to 7 from the culture container and spread (transfer) them to the tray for freeze drying. There are various sizes of freeze-drying trays, but they can be selected and used as necessary.
２．フリーズドライ
前記トレーに移し替えた霊芝菌糸体を培養済みの大豆培地ごと、フリーズドライ装置でフリーズドライする。フリーズドライ装置は汎用のものでも他のものでもよい。
2. Freeze-drying The Reishi mushroom mycelium transferred to the tray is freeze-dried with the freeze-drying device together with the cultured soybean medium. The freeze-drying device may be a general-purpose device or another device.
３．破砕、バルク、大豆を含む霊芝菌糸体健康食品
フリーズドライした大豆を含む霊芝菌糸体を破砕（粉砕）し、顆粒、粉末（微粉末を含む）、カプセル、液体といった所望の形態にする。一回の服用量ずつに個別包装することもできる。前記大豆を含む霊芝菌糸体の破砕には粉砕機、特に超微粉砕機を使用することができる。
3. Crush, bulk, Ganoderma mycelium health food containing soybeans Ganoderma mycelium containing freeze-dried soybeans is crushed (pulverized) into desired forms such as granules, powders (including fine powders), capsules, and liquids. It can also be individually packaged for each dose. A pulverizer, particularly an ultra-fine pulverizer can be used for crushing the ganoderma mycelium containing soybean.
前記顆粒、粉末、カプセル、液体といった形態にして個別包装するのは、製薬会社や食品会社で行うことができる。その場合は、フリーズドライした大豆を含む霊芝菌糸体を破砕（粉砕）した状態で、あるいはバルクの状態で、製薬会社や食品会社に送ることになる。 Individual packaging in the form of granules, powders, capsules, and liquids can be performed by a pharmaceutical company or a food company. In that case, the ganoderma mycelium containing freeze-dried soybeans is crushed (pulverized) or sent in bulk to a pharmaceutical company or food company.
Claims (6)
浸漬済みの大豆を大豆培地として培養容器に収容する工程と、
霊芝菌糸体が液体に分散された霊芝菌糸体の液種を、接種機で培養容器内の大豆培地に噴射して接種する工程と、
霊芝菌糸体接種後の培養容器を培養室で培養する工程を備えた、
ことを特徴とする大豆培地での霊芝菌糸体培養方法。 Immersing at least soybean in the liquid;
Storing the soaked soybean in a culture container as a soybean medium;
A step of inoculating the liquid species of Ganoderma mycelium in which Ganoderma mycelium is dispersed in a liquid by injecting it onto a soybean medium in a culture vessel with an inoculator;
Comprising the step of culturing the culture container after inoculation of the reishi mycelium in the culture room,
A method for cultivating Ganoderma mycelium in a soybean medium.
霊芝菌糸体の液種を培養容器内の底側の大豆培地まで噴射する、
ことを特徴とする大豆培地での霊芝菌体培養方法。 In the method of cultivating Ganoderma mycelium in the soybean medium according to claim 1,
Spray the liquid species of Ganoderma mycelium to the bottom soybean medium in the culture vessel,
A method for cultivating reishi mushroom cells in a soybean medium,
接種機からの一回の液種噴射量が一定である、
ことを特徴とする大豆培地での霊芝菌体培養方法。 In the method of cultivating ganoderma cells in the soybean medium according to claim 1 or 2,
The amount of liquid injection from the inoculator is constant,
A method for cultivating reishi mushroom cells in a soybean medium,
請求項１から請求項３のいずれかに記載の大豆培地での霊芝菌糸体培養方法で培養された霊芝菌糸体及び培養後の大豆培地が、フリーズドライされたものである、
ことを特徴とする大豆を含む霊芝菌糸体健康食品。 In health foods containing cultured reishi mycelium,
Ganoderma mycelium cultivated by the Ganoderma mycelium culture method in the soybean medium according to any one of claims 1 to 3, and the soybean medium after cultivation are freeze-dried.
Ganoderma mycelium health food containing soy.
フリーズドライされた培養霊芝菌糸体及び培養後の大豆培地を粉砕して、粉末、顆粒、錠剤、液状、カプセルのいずれかの形態としたものである、
ことを特徴とする大豆を含む霊芝菌糸体健康食品。 In the Ganoderma mycelium health food containing soybean according to claim 4,
The freeze-dried cultured ganoderma mycelium and the cultured soybean medium are pulverized into a powder, granule, tablet, liquid, or capsule form.
Ganoderma mycelium health food containing soy.
ことを特徴とする大豆を含む霊芝菌糸体健康食品。 Ganoderma mycelium health food containing soybean according to claim 5 is individually packaged for each dose.
Ganoderma mycelium health food containing soy.
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| CN110583366A (en) * | 2019-09-30 | 2019-12-20 | 山东瑞蕈天库菌种开发有限公司 | Synthetic particle stock culture medium and preparation method and application thereof |
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| CN111052985A (en) * | 2019-12-31 | 2020-04-24 | 北京大道东圣生物科技有限公司 | Myxomycete hypha biological extract and preparation method and application thereof |
| CN115530005B (en) * | 2022-10-14 | 2023-06-23 | 韶关市五马寨菌业有限公司 | Method for rapid propagation of sweet ganoderma lucidum through tissue culture |
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