JP2016095408A - 走査型顕微鏡 - Google Patents
走査型顕微鏡 Download PDFInfo
- Publication number
- JP2016095408A JP2016095408A JP2014231691A JP2014231691A JP2016095408A JP 2016095408 A JP2016095408 A JP 2016095408A JP 2014231691 A JP2014231691 A JP 2014231691A JP 2014231691 A JP2014231691 A JP 2014231691A JP 2016095408 A JP2016095408 A JP 2016095408A
- Authority
- JP
- Japan
- Prior art keywords
- objective lens
- light
- exit pupil
- optical system
- pupil position
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001747 pupil Anatomy 0.000 claims abstract description 72
- 230000003287 optical effect Effects 0.000 claims abstract description 69
- 238000005286 illumination Methods 0.000 claims abstract description 15
- 238000000926 separation method Methods 0.000 claims description 9
- 230000005284 excitation Effects 0.000 abstract description 61
- 238000000034 method Methods 0.000 abstract description 3
- 238000002955 isolation Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 22
- 238000010586 diagram Methods 0.000 description 17
- 238000001514 detection method Methods 0.000 description 11
- 238000001917 fluorescence detection Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 230000005499 meniscus Effects 0.000 description 2
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B13/00—Optical objectives specially designed for the purposes specified below
- G02B13/0095—Relay lenses or rod lenses
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
- G02B21/025—Objectives with variable magnification
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
- G02B27/14—Beam splitting or combining systems operating by reflection only
- G02B27/141—Beam splitting or combining systems operating by reflection only using dichroic mirrors
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Lenses (AREA)
- Spectroscopy & Molecular Physics (AREA)
Abstract
Description
対物レンズ4
s r d nd vd 有効径
1 ∞ 6.05 1.33304 55.79
2 ∞ 9.00
3 ∞ 3.50 1.51633 64.14 16.0
4 21.0497 0.50 16.0
5 -10.5065 4.30 1.6779 55.34 15.0
6 -11.3866 2.00 1.48749 70.23 13.0
7 -7.024 8.50 10.0
8 7.801 7.00 1.58144 40.75 9.0
9 -37.142 8.84 14.0
10 37.142 1.50 1.59551 39.24 21.0
11 -40.593 6.20 1.43875 94.99 21.0
12 18.5902 0.20 22.0
13 -121.955 6.30 1.48749 70.23 26.0
14 22.4011 -3.90 25.0
2 XYスキャナ
2c、8c 共役面
3 ダイクロイックミラー
3e 端部
4、11 対物レンズ
5 非共焦点検出部
6、12、13、14 リレー光学系
7 PMT
8 受光面
9 胴付き面
10、20、30 多光子励起顕微鏡
S 試料
P 射出瞳位置
Claims (6)
- 照明光で試料を走査する走査手段と、
射出瞳位置を外部に有し、前記走査手段で偏向された照明光を前記試料に照射する対物レンズと、
前記対物レンズと前記射出瞳位置との間に配置され、前記照明光と前記試料からの観察光の一方を反射させ他方を透過させる光分離素子と、を備える
ことを特徴とする走査型顕微鏡。 - 請求項1に記載の走査型顕微鏡において、さらに、
前記走査手段を介すことなく、前記対物レンズ及び前記光分離素子を介して、前記観察光を検出するノンデスキャン検出器を備える
ことを特徴とする走査型顕微鏡。 - 請求項2に記載の走査型顕微鏡において、さらに、
前記対物レンズの射出瞳位置と前記ノンデスキャン検出器との間に配置され、前記ノンデスキャン検出器の受光面を前記試料と前記射出瞳位置との間に投影する第1のリレー光学系を備える
ことを特徴とする走査型顕微鏡。 - 請求項3に記載の走査型顕微鏡において、さらに、
前記第1のリレー光学系が前記ノンデスキャン検出器の受光面を投影する位置を調整する第1の調整手段を備える
ことを特徴とする走査型顕微鏡。 - 請求項1乃至請求項4のいずれか1項に記載の走査型顕微鏡において、さらに、
前記走査手段を前記射出瞳位置に投影する第2のリレー光学系と、
前記第2のリレー光学系が前記走査手段を投影する位置を調整する第2の調整手段と、を備える
ことを特徴とする走査型顕微鏡。 - 請求項5に記載の走査型顕微鏡において、さらに、
前記第2のリレー光学系の投影倍率を調整する第3の調整手段と、を備える
ことを特徴とする走査型顕微鏡。
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014231691A JP6367690B2 (ja) | 2014-11-14 | 2014-11-14 | 走査型顕微鏡 |
US14/933,944 US9798128B2 (en) | 2014-11-14 | 2015-11-05 | Scanning microscope |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014231691A JP6367690B2 (ja) | 2014-11-14 | 2014-11-14 | 走査型顕微鏡 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2016095408A true JP2016095408A (ja) | 2016-05-26 |
JP6367690B2 JP6367690B2 (ja) | 2018-08-01 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2014231691A Active JP6367690B2 (ja) | 2014-11-14 | 2014-11-14 | 走査型顕微鏡 |
Country Status (2)
Country | Link |
---|---|
US (1) | US9798128B2 (ja) |
JP (1) | JP6367690B2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019191267A (ja) * | 2018-04-19 | 2019-10-31 | オリンパス株式会社 | 顕微鏡対物レンズ |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10732414B2 (en) * | 2016-08-17 | 2020-08-04 | Microsoft Technology Licensing, Llc | Scanning in optical systems |
KR20200108666A (ko) | 2019-03-11 | 2020-09-21 | 삼성전자주식회사 | 영상의 횡이동이 가능한 디스플레이 장치 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6072624A (en) * | 1992-01-09 | 2000-06-06 | Biomedical Photometrics Inc. | Apparatus and method for scanning laser imaging of macroscopic samples |
JP2001221952A (ja) * | 2000-02-08 | 2001-08-17 | Olympus Optical Co Ltd | 顕微鏡光学系 |
US20070109546A1 (en) * | 2005-11-15 | 2007-05-17 | Doron Meshulach | Scanning Microscopy |
JP2009294385A (ja) * | 2008-06-04 | 2009-12-17 | Olympus Corp | 顕微鏡装置 |
JP2012189792A (ja) * | 2011-03-10 | 2012-10-04 | Olympus Corp | 顕微鏡装置 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6882417B2 (en) * | 2002-03-21 | 2005-04-19 | Applied Materials, Inc. | Method and system for detecting defects |
JP4576137B2 (ja) * | 2004-03-19 | 2010-11-04 | オリンパス株式会社 | 顕微鏡 |
US8228601B2 (en) * | 2008-05-13 | 2012-07-24 | Applied Materials Israel, Ltd. | Scanning microscopy using inhomogeneous polarization |
WO2010095263A1 (ja) * | 2009-02-23 | 2010-08-26 | オリンパス株式会社 | レーザ顕微鏡 |
JP5321305B2 (ja) | 2009-07-15 | 2013-10-23 | 株式会社ニコン | 走査型顕微鏡 |
-
2014
- 2014-11-14 JP JP2014231691A patent/JP6367690B2/ja active Active
-
2015
- 2015-11-05 US US14/933,944 patent/US9798128B2/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6072624A (en) * | 1992-01-09 | 2000-06-06 | Biomedical Photometrics Inc. | Apparatus and method for scanning laser imaging of macroscopic samples |
JP2001221952A (ja) * | 2000-02-08 | 2001-08-17 | Olympus Optical Co Ltd | 顕微鏡光学系 |
US20070109546A1 (en) * | 2005-11-15 | 2007-05-17 | Doron Meshulach | Scanning Microscopy |
JP2009294385A (ja) * | 2008-06-04 | 2009-12-17 | Olympus Corp | 顕微鏡装置 |
JP2012189792A (ja) * | 2011-03-10 | 2012-10-04 | Olympus Corp | 顕微鏡装置 |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019191267A (ja) * | 2018-04-19 | 2019-10-31 | オリンパス株式会社 | 顕微鏡対物レンズ |
JP7107519B2 (ja) | 2018-04-19 | 2022-07-27 | 株式会社エビデント | 顕微鏡対物レンズ |
US11402618B2 (en) | 2018-04-19 | 2022-08-02 | Olympus Corporation | Microscope objective |
Also Published As
Publication number | Publication date |
---|---|
US20160139395A1 (en) | 2016-05-19 |
JP6367690B2 (ja) | 2018-08-01 |
US9798128B2 (en) | 2017-10-24 |
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