JP2013005803A5 - - Google Patents

Download PDF

Info

Publication number
JP2013005803A5
JP2013005803A5 JP2012179420A JP2012179420A JP2013005803A5 JP 2013005803 A5 JP2013005803 A5 JP 2013005803A5 JP 2012179420 A JP2012179420 A JP 2012179420A JP 2012179420 A JP2012179420 A JP 2012179420A JP 2013005803 A5 JP2013005803 A5 JP 2013005803A5
Authority
JP
Japan
Prior art keywords
amplification
steps
nucleic acid
target nucleic
performed simultaneously
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2012179420A
Other languages
English (en)
Japanese (ja)
Other versions
JP5685561B2 (ja
JP2013005803A (ja
Filing date
Publication date
Application filed filed Critical
Publication of JP2013005803A publication Critical patent/JP2013005803A/ja
Publication of JP2013005803A5 publication Critical patent/JP2013005803A5/ja
Application granted granted Critical
Publication of JP5685561B2 publication Critical patent/JP5685561B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

JP2012179420A 2000-05-19 2012-08-13 核酸の検出用物質及び検出方法 Expired - Fee Related JP5685561B2 (ja)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US20571200P 2000-05-19 2000-05-19
US60/205,712 2000-05-19
US24039800P 2000-10-14 2000-10-14
US60/240,398 2000-10-14
US28283101P 2001-04-10 2001-04-10
US60/282,831 2001-04-10

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2001586611A Division JP5138141B2 (ja) 2000-05-19 2001-05-18 核酸の検出用物質及び検出方法

Publications (3)

Publication Number Publication Date
JP2013005803A JP2013005803A (ja) 2013-01-10
JP2013005803A5 true JP2013005803A5 (fr) 2014-07-31
JP5685561B2 JP5685561B2 (ja) 2015-03-18

Family

ID=27394838

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2001586611A Expired - Lifetime JP5138141B2 (ja) 2000-05-19 2001-05-18 核酸の検出用物質及び検出方法
JP2012179420A Expired - Fee Related JP5685561B2 (ja) 2000-05-19 2012-08-13 核酸の検出用物質及び検出方法

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP2001586611A Expired - Lifetime JP5138141B2 (ja) 2000-05-19 2001-05-18 核酸の検出用物質及び検出方法

Country Status (10)

Country Link
US (6) US7422850B2 (fr)
EP (1) EP1349954B1 (fr)
JP (2) JP5138141B2 (fr)
CN (1) CN1592792B (fr)
AT (1) ATE497018T1 (fr)
AU (1) AU7125401A (fr)
CA (1) CA2409309A1 (fr)
DE (1) DE60143960D1 (fr)
HK (1) HK1061410A1 (fr)
WO (1) WO2001090417A2 (fr)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2409309A1 (fr) * 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materiaux et procedes de detection d'acides nucleiques
JP4499987B2 (ja) * 2000-10-14 2010-07-14 エラジェン バイオサイエンシズ インコーポレイテッド 非−標準塩基を使用する固体支持体アッセイ系及び方法
EP1236804A1 (fr) * 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh Méthode pour la détermination d'un acide nucléique utilisant un controle
CA2476564C (fr) * 2002-03-01 2012-05-15 Integrated Dna Technologies, Inc. Amplification polynomiale d'acides nucleiques
EP1590482B1 (fr) * 2003-01-17 2008-12-10 Eragen Biosciences, Inc. Amplification d'acides nucleiques au moyen de bases non standard
US7820808B2 (en) 2003-04-01 2010-10-26 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
CN1280428C (zh) * 2003-05-19 2006-10-18 清华大学 一种基于微小颗粒的生物芯片***及其应用
US7282333B2 (en) * 2003-10-20 2007-10-16 Promega Corporation Methods and compositions for nucleic acid analysis
WO2005081776A2 (fr) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Substances et procedes de detection du syndrome respiratoire aigu severe (sras)
EP1733054A2 (fr) * 2004-04-06 2006-12-20 Epigenomics AG Procede de quantification de l'adn methyle
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
WO2006095550A1 (fr) * 2005-03-04 2006-09-14 Kyoto University Amorce de pcr, methode mettant en application cette amorce et produit amplifie par pcr, dispositif et complexe adn-proteine mettant en application ce produit amplifie par pcr
WO2006101913A2 (fr) * 2005-03-18 2006-09-28 Eragen Biosciences, Inc. Procedes destines a detecter des especes et des sous-especes multiples de neiserria
CA2610863C (fr) 2005-06-07 2017-01-17 Eragen Biosciences, Inc. Methodes de detection et de typage d'acides nucleiques
US20120122095A1 (en) * 2006-01-12 2012-05-17 Eragen Biosciences, Inc. Materials and methods for the detection of anthrax related toxin genes
US20070264694A1 (en) * 2006-04-07 2007-11-15 Eragen Biosciences, Inc. Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
WO2008061196A2 (fr) * 2006-11-15 2008-05-22 Eragen Biosciences, Inc. Procédés et kits de détection de l'acide nucléique jak2
EP2147118A4 (fr) * 2007-04-27 2010-09-01 Eragen Biosciences Inc Matériaux et procédés de détection du virus de l'hépatite c
US7678894B2 (en) * 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
US9163053B2 (en) 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
JP2009178159A (ja) * 2007-11-05 2009-08-13 Hitachi Plant Technologies Ltd 核酸配列の検出方法及び核酸配列検出基板
KR100978215B1 (ko) * 2008-02-28 2010-08-26 주식회사 인트론바이오테크놀로지 건조형태의 ｐｃｒ 반응용 조성물에 기반한 ｐｃｒ의 최적증폭 조건 탐색 및 설정에 활용될 수 있는 키트 및 그것의제조방법
US9249455B2 (en) * 2008-04-18 2016-02-02 Luminex Corporation Methods for detection and quantification of small RNA
EP2133434A1 (fr) 2008-06-12 2009-12-16 Institut National de la Santé et de la Recherche Médicale (INSERM) Procédé pour la détection d'un acide nucléique cible
JP5596684B2 (ja) * 2008-08-12 2014-09-24 エフ．ホフマン−ラ ロシュ アーゲー プルーフリーディング・プライマー伸長
WO2010030461A2 (fr) * 2008-09-12 2010-03-18 Promega Corporation Évaluation de l’expression de gènes endogènes et exogènes
US20100112557A1 (en) * 2008-11-03 2010-05-06 Applied Biosystems Inc. Method for high resolution melt genotyping
US20100112565A1 (en) * 2008-11-03 2010-05-06 Life Technologies Corporation Methods, kits, and reaction mixtures for high resolution melt genotyping
JP5715068B2 (ja) 2009-01-30 2015-05-07 マイクロニクス， インコーポレイテッド 携帯型高利得蛍光検出システム
CA2778449C (fr) * 2009-10-23 2017-06-20 Luminex Corporation Amorces d'amplification avec des bases non-standard ayant une specificite de reaction augmentee
WO2011053845A2 (fr) 2009-10-30 2011-05-05 Illumina, Inc. Microvaisseaux, microparticules et leurs procédés de fabrication et d'utilisation
US9758817B2 (en) * 2010-01-13 2017-09-12 Agilent Technologies, Inc. Method for identifying a nucleic acid in a sample
KR101851117B1 (ko) 2010-01-29 2018-04-23 마이크로닉스 인코포레이티드. 샘플-투-앤서 마이크로유체 카트리지
EP2556458B1 (fr) 2010-04-09 2020-06-03 Life Technologies Corporation Outil de visualisation pour données de génotypage obtenues par qpcr
MX340258B (es) * 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
CN104245959B (zh) 2012-03-05 2017-10-13 Seegene株式会社 基于探测和标记寡核苷酸切割及延伸试验的从靶核酸序列的核苷酸变异检测
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP3549674B1 (fr) 2012-12-21 2020-08-12 PerkinElmer Health Sciences, Inc. Films à faible élasticité pour utilisation micro-fluidique
WO2014100725A1 (fr) 2012-12-21 2014-06-26 Micronics, Inc. Système portable de détection par fluorescence et cartouche de microanalyse
US10065186B2 (en) 2012-12-21 2018-09-04 Micronics, Inc. Fluidic circuits and related manufacturing methods
EP2770065B1 (fr) 2013-02-25 2017-12-13 Seegene, Inc. Détection de variations de nucléotides sur une séquence d'acide nucléique cible
AU2013202788B2 (en) * 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
JP6484222B2 (ja) 2013-05-07 2019-03-13 マイクロニクス， インコーポレイテッド 核酸の調製および分析のためのデバイス
EP2994750B1 (fr) 2013-05-07 2020-08-12 PerkinElmer Health Sciences, Inc. Dispositifs microfluidiques et méthodes de séparation du sérum et de compatibilité croisée du sang
JP6472788B2 (ja) 2013-05-07 2019-02-20 マイクロニクス， インコーポレイテッド 粘土鉱物およびアルカリ性溶液を使用して核酸含有試料を調製するための方法
JP6050555B2 (ja) 2013-07-15 2016-12-21 シージーン アイエヌシー Ｐｔｏの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出
US9657332B2 (en) 2013-08-09 2017-05-23 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
CN105705657B (zh) 2013-10-18 2020-07-28 Seegene株式会社 基于利用杂交-捕捉和模板化寡核苷酸的探测和标记寡核苷酸切割及延伸分析的在固相中的靶核酸序列检测
WO2015147370A1 (fr) 2014-03-28 2015-10-01 Seegene, Inc. Détection de séquences d'acide nucléique cible à l'aide de différentes températures de détection
EP4299760A3 (fr) 2014-08-11 2024-02-21 Luminex Corporation Sondes de discrimination et de multiplexage améliorés dans des analyses d'acide nucléique
WO2018119443A1 (fr) * 2016-12-23 2018-06-28 The Regents Of The University Of California Procédé et dispositif de fusion numérique haute résolution
CN106950326B (zh) * 2017-03-10 2018-05-29 武汉大学 一种化学标记结合lc-ms的方法及其在核苷酸分析中的应用
WO2018175296A2 (fr) * 2017-03-20 2018-09-27 President And Fellows Of Harvard College Cascade de synthèse d'acide nucléique programmable
EP3688188A4 (fr) 2017-09-29 2021-06-16 Seegene, Inc. Détection de séquences d'acides nucléiques cibles par analyse par clivage et extension de pto
WO2019064483A1 (fr) * 2017-09-29 2019-04-04 積水メディカル株式会社 Procédé de détection d'une substitution de base unique à l'aide de la chromatographie par échange d'ions
WO2019178337A1 (fr) 2018-03-14 2019-09-19 Luminex Corporation Amplification multiplexée d'acides nucléiques par abaissement de la température de dénaturation et augmentation de la température d'hybridation
CA3095112A1 (fr) 2018-04-20 2019-10-24 Seegene, Inc. Procede et appareil pour la detection d'une pluralite de sequences d'acides nucleiques cibles dans un echantillon
WO2020014296A1 (fr) 2018-07-12 2020-01-16 Luminex Corporation Systèmes et procédés permettant d'effectuer des processus de préparation et d'analyse d'échantillons variables
CN113913497B (zh) * 2021-02-03 2024-05-28 山东舜丰生物科技有限公司 利用碱基修饰的单链核酸进行靶核酸检测的方法
WO2023043280A1 (fr) 2021-09-17 2023-03-23 주식회사 씨젠 Détection d'une séquence d'acide nucléique cible à l'aide d'un oligonucléotide marqueur porteur de base non naturelle synthétique

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
US5856092A (en) 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
NZ235032A (en) 1989-08-31 1993-04-28 Dow Chemical Co Constrained geometry complexes of titanium, zirconium or hafnium comprising a substituted cyclopentadiene ligand; use as olefin polymerisation catalyst component
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes
US6007984A (en) * 1989-11-01 1999-12-28 Zeneca Limited Detection of DNA/RNA by fluorescence polarization
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US5965364A (en) * 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE4129653A1 (de) 1991-09-06 1993-03-11 Boehringer Mannheim Gmbh Verfahren zum nachweis aehnlicher nukleinsaeuren
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
AU3933393A (en) 1993-03-17 1994-10-11 City Of Hope Nucleic acid sequences having single stranded ends
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5654138A (en) 1993-05-14 1997-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Von hippel-lindau (VHL) disease gene and corresponding cDNA and methods for detecting carriers of the VHL disease gene
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5589329A (en) 1993-11-15 1996-12-31 Ambion, Inc. Methods and compositions for detecting base pair mismatches
JP3175110B2 (ja) 1994-02-07 2001-06-11 オーキッド・バイオサイエンシーズ・インコーポレイテッド リガーゼ／ポリメラーゼ媒体された単一ヌクレオチド多型のジェネティックビットアナリシスおよび遺伝子解析におけるその使用
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5866337A (en) 1995-03-24 1999-02-02 The Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5980861A (en) 1996-03-12 1999-11-09 University Of Massachusetts Chelator compositions and methods of synthesis thereof
WO1997035033A1 (fr) 1996-03-19 1997-09-25 Molecular Tool, Inc. Methode de determination de la sequence nucleotidique d'un polynucleotide
US5780233A (en) 1996-06-06 1998-07-14 Wisconsin Alumni Research Foundation Artificial mismatch hybridization
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
JP4185185B2 (ja) * 1997-05-08 2008-11-26 征夫 軽部 部分二重鎖ｄｎａを利用したｄｎａの検出方法
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
EP1032701A1 (fr) * 1997-10-28 2000-09-06 The Regents of The University of California Detection de mesappariements de bases d'adn par cytometrie de flux
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
JP4471494B2 (ja) 1998-05-14 2010-06-02 ルミネックス コーポレイション ダイオードレーザをベースとした測定装置
ATE423314T1 (de) 1998-06-24 2009-03-15 Illumina Inc Dekodierung von matrixartig-angeordneten sensoren durch mikropartikel
WO2000039345A1 (fr) 1998-12-30 2000-07-06 Dana-Farber Cancer Institute, Inc. Reseau de balayage de mutation et methode d'utilisation
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
JP2001204497A (ja) 2000-01-20 2001-07-31 Japan Science & Technology Corp 蛍光共鳴エネルギー移動を利用した、ｄｎａハイブリダイゼーション法によるｄｎａ検出方法
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
CA2409309A1 (fr) * 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materiaux et procedes de detection d'acides nucleiques
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
JP4499987B2 (ja) 2000-10-14 2010-07-14 エラジェン バイオサイエンシズ インコーポレイテッド 非−標準塩基を使用する固体支持体アッセイ系及び方法
US6843657B2 (en) * 2001-01-12 2005-01-18 Litton Systems Inc. High speed, high density interconnect system for differential and single-ended transmission applications
GB0129450D0 (en) * 2001-12-08 2002-01-30 Koninkl Philips Electronics Nv Trenched semiconductor devices and their manufacture
US20030194705A1 (en) 2002-03-01 2003-10-16 Schroth Gary P. Methods of using unnatural nucleobases for decoding

Similar Documents

Publication Publication Date Title
JP2013005803A5 (fr)
JP2017514877A5 (fr)
JP2016525888A5 (fr)
JP2012143233A5 (fr)
JP2013033060A5 (fr)
EP4219744A3 (fr) Procédés de préparation d'acides nucléiques pour le séquençage
JP2015520667A5 (fr)
JP2013541578A5 (fr)
JP2011102971A5 (fr)
JP2012255926A5 (fr)
JP2013531018A5 (fr)
JP2015503422A5 (fr)
EP2971123A4 (fr) Amplification d'acide nucléique
JP2012098988A5 (fr)
GB201219137D0 (en) Direct nucleic acid amplification kit, reagent and method
BR112014026149A2 (pt) método para a preparação de 2,4-ácido dihidroxibutírico (2,4-dhb) e microrganismo.
JP2012098270A5 (fr)
EP2774978A4 (fr) Procédé d'amplification d'acide nucléique, substrat pour acide nucléique, procédé et dispositif d'analyse d'acide nucléique
WO2015112948A3 (fr) Procédés de détermination d'une séquence nucléotidique
JP2014507463A5 (fr)
JP2011082173A5 (fr)
EP3071713A4 (fr) Amplification des acides nucléiques
JP2013129938A5 (fr)
JP2014189647A5 (fr)
JP2012209496A5 (fr)