JP2012127969A - 画像データ処理の方法及びシステム - Google Patents
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Abstract
【解決手段】この方法、記憶媒体、システムの実施形態は、背景信号測定と、分類染料放射及びクラスタ拒絶を使用する粒子識別と、画像間整列と、画像間粒子相関と、リポータ放射の蛍光積分と、画像平面正規化とのうちの1つ又は複数のステップを実行する構成を含む。
【選択図】図3
Description
新しいx座標=[(x−xorigin)2+(y−yorigin)2]の平方根×cos(回転
角度)+xorigin+xtranslation(適用可能な場合に)+0.5 (1)
新しいy座標=[(x−xorigin)2+(y−yorigin)2]の平方根×sin(回転
角度)+yorigin+ytranslation(適用可能な場合に)+0.5 (2)
検討中の画素の値を、空白の画像バッファの、新しいx座標とy座標の画素にコピーすることができる。回転を意図された非基準画像を処理した後に、新しい合成擬似カラー画像を作成することができる。一般に、回転処理に関する上で概要を示したステップを、各非基準画像にまたがる色の相違を最小にするために繰り返す。最終的な回転値を、非基準画像ごとに、後続結像に関する調整テーブルなどの適切なデータ構造に保存することができる。
複数の画像を、複数の異なるカラー・チャネルによって形成することができ、その結果、本明細書で説明する方法は、3つの画像又は赤、緑、青のカラー・チャネルの形成には制限されない。
Claims (11)
- 蛍光材料を会合された粒子の画像内の第1の所定のしきい値を超える光学パラメータ値を示す1つ又は複数の画素を識別するために、前記画像を分析する、プロセッサによって実行可能なプログラム命令と、
前記1つ又は複数の識別された画素のセット内の前記光学パラメータの最大値をそれぞれが示す前記セット内の位置を判定する、プロセッサによって実行可能なプログラム命令と、
それに続いて、前記複数の画素が粒子の画像内の単一の粒子又は粒子の画像内の粒子のクランプを示すかどうか決定するために、前記位置のうちの少なくとも1つを囲む複数の画素の前記光学パラメータの強度変化の割合を計算する、プロセッサによって実行可能なプログラム命令と、
強度変化の前記計算された割合に基づいて、さらなる評価に関して前記複数の画素を受け入れるか拒絶する、プロセッサによって実行可能なプログラム命令と
を含む非一時的な記憶媒体。 - 前記複数の画素を受け入れるか拒絶する前記プログラム命令が、
強度変化の前記割合が前記第1の所定のしきい値とは別個の第2の所定のしきい値以上であることを計算する時に、さらなる評価に関して前記複数の画素を受け入れるステップと、
強度変化の前記割合が前記第2の所定のしきい値未満であることを計算する時に、さらなる評価に関して前記複数の画素を拒絶するステップと
を含む請求項1に記載の記憶媒体。 - 強度変化の前記割合を計算する前記プログラム命令が、
前記少なくとも1つの位置を囲む第1の所定の半径の中に配置された複数の画素の第1セットの前記光学パラメータの値を合計するプログラム命令と、
前記少なくとも1つの位置を囲む第2の所定の半径の中に配置された複数の画素の第2セットの前記光学パラメータの値を合計するプログラム命令であって、前記第2の所定の半径が、前記第1の所定の半径より大きい、プログラム命令と、
画素の前記第1セット及び前記第2セットについてそれぞれ計算された前記合計された値の比率を判定するプログラム命令と
を含む請求項1に記載の記憶媒体。 - 前記第1の所定の半径が、前記画像内の単一の粒子の投影された直径とほぼ等しく、前記投影された直径が、前記粒子を画像化するのに使用されるシステムの構成要素構成に基づく請求項3に記載の記憶媒体。
- 前記第2の所定の半径が、前記画像内の単一の粒子の投影された直径より約1.5倍大きく、前記投影された直径が、前記粒子を画像化するのに使用されるシステムの構成要素構成に基づく請求項3に記載の記憶媒体。
- 前記位置を判定する前記プログラム命令が、前記光学パラメータの最大値をそれぞれが示す1つ又は複数の識別された画素の前記セットの中でピーク画素を確かめるプログラム命令を含む請求項1に記載の記憶媒体。
- 前記位置を判定する前記プログラム命令が、1つ又は複数の識別された画素の前記セットのうちの少なくとも1つの中で、前記少なくとも1つのセットに対応する前記ピーク画素内と前記ピーク画素を囲む第2の所定の半径によって含まれる前記ピーク画素に隣接する画素内の前記光学パラメータの値に基づいて、重心位置を計算するプログラム命令をさらに含む請求項6に記載の記憶媒体。
- 前記位置を判定する前記プログラム命令が、前記重心位置の寸法が前記画像内の画素幅の約50%より大きいと判定する時に、前記少なくとも1つのセット内の前記光学パラメータの前記最大値を示す前記位置として前記重心位置を割り当てるプログラム命令をさらに含む請求項7に記載の記憶媒体。
- 前記ピーク画素のうちの2つの間の距離を計算するプログラム命令と、
前記距離が第2の所定のしきい値未満であることを計算する時に、前記2つのピーク画素のうちの1つに対応する画素のセットをさらなる評価に関して拒絶するプログラム命令と
をさらに含む請求項6に記載の記憶媒体。 - 前記画素のセットを拒絶する前記プログラム命令が、
前記2つのピーク画素に対応する画素のセットごとに前記光学パラメータの強度変化の割合を計算するプログラム命令と、
前記画素のセットごとに計算された前記強度変化の割合に基づいて前記画素のセットをを拒絶するプログラム命令をさらに含む請求項9に記載の記憶媒体。 - 前記複数の画素の前記第1セット及び前記第2セットの前記光学パラメータの値を合計する前記ステップと、異なる所定の半径について前記合計された値の比率を決定する前記ステップとを繰り返すプログラム命令をさらに含む請求項3に記載の記憶媒体。
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