JP2011502143A - Bactericidal composition comprising peracid and acetylsalicylic acid - Google Patents
Bactericidal composition comprising peracid and acetylsalicylic acid Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
Abstract
【課題】少なくとも２１日以上の長期間にかけて前記希釈溶液内の過酢酸の濃度を有効濃度レベル以上に維持させることができる殺菌組成物及び常時殺菌組成物の製造方法を提供する。
【解決手段】本発明は、酢酸、過酸化水素、過酢酸、及びアセチルサリチル酸を含む酸性の殺菌組成物を提供する。前記殺菌組成物中のアセチルサリチル酸の重量比は０．１％ないし１０％でありうる。本発明はまた、酢酸、過酸化水素、過酢酸を含有する第１溶液、及びアセチルサリチル酸を含有する第２溶液を混合することを含む殺菌組成物の製造方法を提供する。前記希釈溶液でアセチルサリチル酸は、持続的に水和されつつ酢酸を供与でき、またその水和化物であるサリチル酸は追加的な殺菌剤として作用できる。結果的に、前記殺菌組成物は平衡組成物を形成し、精製水により希釈される場合にも３週以上の長期間保存して使われうる。The present invention provides a sterilizing composition capable of maintaining the concentration of peracetic acid in the diluted solution at an effective concentration level or more over a long period of at least 21 days and a method for producing a constantly sterilizing composition.
The present invention provides an acidic germicidal composition comprising acetic acid, hydrogen peroxide, peracetic acid, and acetylsalicylic acid. The weight ratio of acetylsalicylic acid in the sterilizing composition may be 0.1% to 10%. The present invention also provides a method for producing a bactericidal composition comprising mixing a first solution containing acetic acid, hydrogen peroxide, peracetic acid, and a second solution containing acetylsalicylic acid. In the diluted solution, acetylsalicylic acid can donate acetic acid while being continuously hydrated, and hydrated salicylic acid can act as an additional fungicide. As a result, the bactericidal composition forms an equilibrium composition and can be used after being stored for a long period of 3 weeks or more even when diluted with purified water.
Description
本発明は殺菌組成物に係り、特に、ピンセット、キャピラリーチューブ、シリコンチューブ、ビニルチューブ、鉗子、シリコンキャップ、シリコン栓、外科用メス、メス取っ手、内視鏡などの一般的な医療装備の殺菌及び消毒に使われる殺菌組成物に関する。 The present invention relates to a sterilizing composition, and in particular, sterilization and general medical equipment such as tweezers, capillary tubes, silicon tubes, vinyl tubes, forceps, silicon caps, silicon stoppers, surgical scalpels, scalpel handles, endoscopes and the like. The present invention relates to a sterilizing composition used for disinfection.
医療装備の正しい使用及び事後管理は、患者を診療する行為の質的レベルを向上させるために必須に要求される。そのなかでも医療装備の洗浄、殺菌、及び消毒は、患者を２次感染の危険から保護するためのものであって、特に重要視されている。通例的に医療装備の殺菌及び消毒は、殺菌消毒剤原液を希釈した溶液を使用して行われている。
現在、医療装備の殺菌及び消毒に最も広く使われる殺菌消毒剤としては、塩素系、有機酸系、アルデヒド系がある。
Proper use and subsequent management of medical equipment is essential to improve the quality level of the practice of treating patients. Among them, cleaning, sterilization, and disinfection of medical equipment are particularly important because they protect patients from the risk of secondary infection. Typically, medical equipment is sterilized and disinfected using a diluted solution of a disinfectant stock solution.
Currently, the most widely used disinfectants for sterilization and disinfection of medical equipment include chlorine, organic acid, and aldehyde.
塩素系殺菌消毒剤として最も代表的なものは、次亜塩素酸ナトリウムである。次亜塩素酸ナトリウムは、広範囲な殺菌スペクトルを持つ長所があるが、廃棄時に環境汚染を誘発する恐れがある。
有機酸系殺菌消毒剤は、それ自体としての殺菌及び消毒効果は落ち、単に部分的な添加効果があるだけである。
The most typical chlorine-based disinfectant is sodium hypochlorite. Sodium hypochlorite has the advantage of having a broad sterilization spectrum, but it may induce environmental pollution when discarded.
Organic acid disinfectants have their own disinfection and disinfection effects, but only a partial addition effect.
アルデヒド系殺菌消毒剤の例としては、グルタルアルデヒド、グリオキシル酸、及びホルムアルデヒドを挙げることができる。これらのうち現在グルタルアルデヒドが最も一般的に使われている。グルタルアルデヒドを有効性分とする製剤は広範囲な殺菌スペクトルを持つ長所があるが、刺激的な臭いと共に人体に対する毒性及びアレルギーの問題が提起されている実情である。 Examples of aldehyde disinfectants include glutaraldehyde, glyoxylic acid, and formaldehyde. Of these, glutaraldehyde is currently the most commonly used. The preparation with glutaraldehyde as an effective component has an advantage of having a wide spectrum of bactericidal spectrum, but it is a situation in which the problem of toxicity and allergy to human body has been raised along with an irritating smell.
これにより、最近には過酢酸を有効性分とする殺菌消毒剤の使用が急増しつつある。前記過酢酸は殺菌力に優れ、その究極的な分解物は人体に無害な酢酸、水、酸素であるところ、環境汚染問題から比較的自由である。
通常、殺菌消毒剤として使われる過酢酸は、高濃度の過酢酸組成物として提供される。
前記高濃度の過酢酸組成物は、通常過酢酸０．１〜０．３５％の濃度で使用時に希釈されて、医療装備の殺菌及び消毒に使われている。
As a result, the use of bactericidal disinfectants that have peracetic acid as an effective component is increasing rapidly. The peracetic acid is excellent in sterilizing power, and its ultimate decomposition product is acetic acid, water, and oxygen which are harmless to the human body, and is relatively free from environmental pollution problems.
Typically, peracetic acid used as a disinfectant is provided as a high concentration peracetic acid composition.
The high-concentration peracetic acid composition is usually diluted at a concentration of 0.1 to 0.35% peracetic acid at the time of use, and is used for sterilization and disinfection of medical equipment.
従来の過酢酸組成物は、通常使用時に希釈溶液として調製されて使われ、この場合、前記希釈溶液は１回に限って使われることが一般的である。これは、高濃度の酢酸及び過酸化水素を主成分とする従来の殺菌組成物の場合、精製水により希釈される場合、前記希釈溶液の殺菌力は経時的に減少するところ、一般的に７日以上保存された希釈溶液は殺菌及び消毒効能が顕著に減少するという問題点があるためである。これにより、前記従来の過酢酸組成物を希釈溶液として調製した後で保存されて使用する場合、通常１週間以内に使われるべきという問題点があった。 The conventional peracetic acid composition is prepared and used as a diluting solution during normal use. In this case, the diluting solution is generally used only once. This is because, in the case of a conventional sterilization composition mainly composed of acetic acid and hydrogen peroxide having a high concentration, when diluted with purified water, the sterilizing power of the diluted solution decreases with time. This is because a diluted solution stored for more than a day has a problem that the sterilizing and disinfecting effects are remarkably reduced. Accordingly, when the conventional peracetic acid composition is used after being prepared as a dilute solution, it should be used within one week.
本発明は、前記過酢酸希釈溶液の保管上の問題点を解決するためのものであって、高濃度の過酢酸組成物を希釈して希釈溶液として調製される場合であっても、少なくとも２１日以上の長期間にかけて前記希釈溶液内の過酢酸の濃度を有効濃度レベル以上に維持させることができる殺菌組成物及び常時殺菌組成物の製造方法を提供することを目的とする。 The present invention is for solving the problem of storage of the peracetic acid diluted solution, and even when it is prepared as a diluted solution by diluting a high concentration peracetic acid composition, at least 21 It is an object of the present invention to provide a sterilizing composition capable of maintaining the concentration of peracetic acid in the diluted solution at an effective concentration level or more over a long period of time longer than a day, and a method for producing a constantly sterilizing composition.
本発明の一様態によれば、酢酸及び過酸化水素を利用した殺菌組成物を提供する。前記殺菌組成物は、酢酸、過酸化水素、過酢酸、及びアセチルサリチル酸を含む。
他の実施例で、前記殺菌組成物は、１，１−ジホスホン酸、クエン酸、クエン酸ナトリウム、低級アルコール、精製水をさらに含むことができる。
さらに他の実施例で、前記組成物中のアセチルサリチル酸の重量比は、０．１％ないし１０％である。
According to one aspect of the present invention, a bactericidal composition using acetic acid and hydrogen peroxide is provided. The bactericidal composition includes acetic acid, hydrogen peroxide, peracetic acid, and acetylsalicylic acid.
In another embodiment, the bactericidal composition may further include 1,1-diphosphonic acid, citric acid, sodium citrate, lower alcohol, and purified water.
In yet another embodiment, the weight ratio of acetylsalicylic acid in the composition is 0.1% to 10%.
本発明の他の様態によれば、酢酸及び過酸化水素を用いた殺菌組成物の製造方法を提供する。前記製造方法によれば、酢酸、過酸化水素、過酢酸を含有する第１溶液及びアセチルサリチル酸を含有する第２溶液を混合することによって前記殺菌組成物を製造できる。
他の実施例で、前記第１溶液は、１，１−ジホスホン酸をさらに含み、前記第２溶液は、クエン酸、クエン酸ナトリウム、低級アルコール、精製水をさらに含む。
According to another aspect of the present invention, a method for producing a bactericidal composition using acetic acid and hydrogen peroxide is provided. According to the manufacturing method, the sterilizing composition can be manufactured by mixing a first solution containing acetic acid, hydrogen peroxide, and peracetic acid and a second solution containing acetylsalicylic acid.
In another embodiment, the first solution further includes 1,1-diphosphonic acid, and the second solution further includes citric acid, sodium citrate, lower alcohol, and purified water.
高濃度の酢酸及び過酸化水素を主成分とする従来の殺菌組成物の場合、精製水により希釈される場合、前記希釈溶液の殺菌力は経時的に減少するところ、一般的に７日以上保存された希釈溶液は、殺菌及び消毒効能が顕著に減少するという問題点がある。しかし、本発明による殺菌組成物の場合、前記希釈溶液でアセチルサリチル酸が持続的に水和しつつ酢酸を供与でき、またその水和物であるサリチル酸は追加的な殺菌剤として作用することによって、その希釈溶液の有効期間を２１日以上に延長させる効果がある。 In the case of a conventional sterilizing composition mainly composed of high-concentration acetic acid and hydrogen peroxide, when diluted with purified water, the sterilizing power of the diluted solution decreases with time. The diluted solution has a problem that the sterilization and disinfection effects are remarkably reduced. However, in the case of the bactericidal composition according to the present invention, acetic acid can be provided while acetylsalicylic acid is continuously hydrated in the diluted solution, and the hydrated salicylic acid acts as an additional bactericidal agent. This has the effect of extending the effective period of the diluted solution to 21 days or more.
本発明の他の様態によれば、酢酸及び過酸化水素を用いた殺菌組成物の製造方法を提供する。前記製造方法によれば、酢酸、過酸化水素、過酢酸を含有する第１溶液及びアセチルサリチル酸を含有する第２溶液を混合することによって前記殺菌組成物を製造できる。
他の実施例で、前記第１溶液は、１，１−ジホスホン酸をさらに含み、前記第２溶液は、クエン酸、クエン酸ナトリウム、低級アルコール、精製水をさらに含む。
According to another aspect of the present invention, a method for producing a bactericidal composition using acetic acid and hydrogen peroxide is provided. According to the manufacturing method, the sterilizing composition can be manufactured by mixing a first solution containing acetic acid, hydrogen peroxide, and peracetic acid and a second solution containing acetylsalicylic acid.
In another embodiment, the first solution further includes 1,1-diphosphonic acid, and the second solution further includes citric acid, sodium citrate, lower alcohol, and purified water.
本発明は、酢酸、過酸化水素、及び過酢酸を含有する第１溶液及びアセチルサリチル酸を主成分とする第２溶液が所定の割合で混合された殺菌組成物についてのものである。前記殺菌組成物は、精製水で希釈された希釈溶液で作られて使用され、実際使用時に前記希釈溶液中の過酢酸の濃度は０．０５％ないし０．５％に調整できる。
前記第１溶液での過酢酸は酢酸と過酸化水素とにより作られ、前記第１溶液の原液中には、各成分が一定割合で混合されて下の反応式と共に化学的平衡状態を維持する。
The present invention relates to a sterilizing composition in which a first solution containing acetic acid, hydrogen peroxide, and peracetic acid and a second solution containing acetylsalicylic acid as a main component are mixed at a predetermined ratio. The sterilizing composition is prepared and used in a diluted solution diluted with purified water, and the concentration of peracetic acid in the diluted solution can be adjusted to 0.05% to 0.5% during actual use.
Peracetic acid in the first solution is made of acetic acid and hydrogen peroxide, and in the stock solution of the first solution, each component is mixed at a certain ratio to maintain a chemical equilibrium with the following reaction formula. .
平衡状態の前記第１溶液で、過酢酸及び過酸化水素は水と反応して発生基酸素を発生させ、前記発生基酸素は、酸化作用により殺菌効果を奏する。この場合、過酢酸は短期間の殺菌効果に優れ、過酸化水素は長期間の殺菌効果に優れるという特徴がある。
ただし、前記第１溶液の原液が精製水により希釈される場合、反応式１での平衡が過酢酸の濃度を減少させる方向に移動することがある。したがって、本発明では前記第１溶液の原液を蒸溜水により希釈させる場合にも、前記反応式１による逆反応を抑制して長期間にかけて過酢酸の有効濃度を維持させるように、アセチルサリチル酸を主成分とする第２溶液を前記第１溶液に所定の割合で混合した酸性の過酢酸製剤の平衡組成物及びその製造方法に関する。
In the first solution in an equilibrium state, peracetic acid and hydrogen peroxide react with water to generate generated base oxygen, and the generated base oxygen has a bactericidal effect by an oxidizing action. In this case, peracetic acid has a short-term sterilizing effect and hydrogen peroxide has a long-term sterilizing effect.
However, when the undiluted solution of the first solution is diluted with purified water, the equilibrium in the reaction formula 1 may move in the direction of decreasing the concentration of peracetic acid. Therefore, in the present invention, even when the stock solution of the first solution is diluted with distilled water, acetylsalicylic acid is mainly used so as to suppress the reverse reaction according to the reaction formula 1 and maintain the effective concentration of peracetic acid over a long period of time. The present invention relates to an equilibrium composition of an acidic peracetic acid preparation in which a second solution as a component is mixed with the first solution at a predetermined ratio and a method for producing the same.
前記第１溶液及び第２溶液の混合溶液の殺菌組成物を希釈した希釈溶液で、アセチルサリチル酸は水と反応して、次の反応式のように酢酸イオンを発生させる。 In a diluted solution obtained by diluting the sterilizing composition of the mixed solution of the first solution and the second solution, acetylsalicylic acid reacts with water to generate acetate ions as shown in the following reaction formula.
前記酢酸イオンは水素イオンと反応して、次の反応式のように酢酸を生成させる。 The acetate ion reacts with hydrogen ion to generate acetic acid as shown in the following reaction formula.
結果的に、前記第１溶液及び第２溶液の混合溶液の原液を精製水で希釈した希釈溶液の場合、前記のように、アセチルサリチル酸により生成された酢酸により前記反応式１での平衡を右に移動させることによって、長期間にかける過酢酸の濃度の減少を効果的に防止できる。これに加えて、アセチルサリチル酸の水和反応によって生成されるサリチル酸の殺菌力により、前記希釈溶液の殺菌力が補強されるという長所も持つ。 As a result, in the case of a diluted solution obtained by diluting the stock solution of the mixed solution of the first solution and the second solution with purified water, as described above, the equilibrium in the reaction formula 1 is right by acetic acid generated by acetylsalicylic acid. By moving to, the decrease in peracetic acid concentration over a long period can be effectively prevented. In addition, the sterilizing power of the diluted solution is reinforced by the sterilizing power of salicylic acid generated by the hydration reaction of acetylsalicylic acid.
本発明による過酢酸製剤の殺菌組成物の製造方法は次の通りである。第１溶液及び第２溶液をそれぞれ作る。次いで、前記第１溶液及び第２溶液を所定割合で混合して殺菌消毒剤原液を作る。前記殺菌消毒剤原液を精製水と共に混合して希釈溶液を作る。前記希釈溶液での過酢酸の濃度は、０．０５〜０．５％でありうる。
前記第１溶液の製造のために、精製水８４〜２５％及び酢酸１０〜４０％を攪拌器に投入して一定時間均一に混合した後、過酸化水素５〜３０％を投入して均一に混合する。この場合、前記酢酸及び前記過酸化水素が反応して過酢酸が所定の平衡濃度で生成される。
The manufacturing method of the disinfectant composition of the peracetic acid formulation by this invention is as follows. Make a first solution and a second solution, respectively. Next, the first solution and the second solution are mixed at a predetermined ratio to make a disinfectant stock solution. The stock solution of the disinfectant is mixed with purified water to make a diluted solution. The concentration of peracetic acid in the diluted solution may be 0.05-0.5%.
For the production of the first solution, 84 to 25% purified water and 10 to 40% acetic acid are added to a stirrer and mixed uniformly for a certain period of time, and then 5 to 30% hydrogen peroxide is added and uniformly added. Mix. In this case, the acetic acid and the hydrogen peroxide react to produce peracetic acid at a predetermined equilibrium concentration.
次いで、前記過程で生成された製品の安定化のために、安定剤である１，１−ジホスホン酸を１〜５％の割合で均一に混合する。前記混合物を３５〜４５℃の温度で４〜７時間保温させた後、常温で３日間熟成させることによって前記第１溶液を製造する。
結果的に過酢酸を生成させる平衡反応によって、前記第１溶液は０．０５〜１５％の過酢酸を含有する。
Subsequently, 1,1-diphosphonic acid as a stabilizer is uniformly mixed at a ratio of 1 to 5% in order to stabilize the product produced in the above process. The mixture is kept at a temperature of 35 to 45 ° C. for 4 to 7 hours and then aged for 3 days at room temperature to prepare the first solution.
The first solution contains 0.05 to 15% peracetic acid by an equilibrium reaction that results in the production of peracetic acid.
前記第２溶液は、アセチルサリチル酸の低級アルコールへの溶液を水性緩衝溶液に均一に混合して製造される。前記混合物中のアセチルサリチル酸の重量比は０．１〜１０％でありうる。前記低級アルコールの具体的な例には、メタノール、エタノール、プロピルアルコール、イソプロピルアルコール及びその組み合わせで構成された一群から選択された一つでありうる。この場合、前記混合物中の前記低級アルコールの重量比は２０〜７０％でありうる。 The second solution is prepared by uniformly mixing a solution of acetylsalicylic acid in a lower alcohol with an aqueous buffer solution. The weight ratio of acetylsalicylic acid in the mixture may be 0.1 to 10%. A specific example of the lower alcohol may be one selected from the group consisting of methanol, ethanol, propyl alcohol, isopropyl alcohol, and combinations thereof. In this case, the weight ratio of the lower alcohol in the mixture may be 20 to 70%.
前記水性緩衝溶液の例には、燐酸、クエン酸、燐酸塩、クエン酸塩及びその組み合わせで構成された一群から選択された一つを精製水に混合したものでありうる。前記第２溶液中の燐酸などの電解質の重量比は１〜１０％、精製水の重量比は１０〜７８．９％でありうる。
これに加えて、消毒の対象になる医療機器の表面の損傷を防止するために、前記第２溶液にベンゾトリアゾールを投入して均一に混合できる。この場合、ベンゾトリアゾールの添加量は、前記第２溶液に対して１ないし５重量％の範囲で調整できる。
＜実施例１＞
前記第１溶液及び第２溶液の混合液を希釈した溶液で、経時的な過酢酸含有量の変化を、添加されたアセチルサリチル酸の濃度によって測定した。
Examples of the aqueous buffer solution may include one selected from the group consisting of phosphoric acid, citric acid, phosphate, citrate, and combinations thereof mixed with purified water. The weight ratio of the electrolyte such as phosphoric acid in the second solution may be 1 to 10%, and the weight ratio of purified water may be 10 to 78.9%.
In addition, in order to prevent damage to the surface of the medical device to be disinfected, benzotriazole can be introduced into the second solution and mixed uniformly. In this case, the amount of benzotriazole added can be adjusted in the range of 1 to 5% by weight with respect to the second solution.
<Example 1>
In a solution obtained by diluting the mixed solution of the first solution and the second solution, the change in peracetic acid content over time was measured by the concentration of the added acetylsalicylic acid.
このために、前記第２溶液でのアセチルサリチル酸の投入量を調節して、前記第１溶液及び第２溶液の最終混合物内でのアセチルサリチル酸の濃度がそれぞれ０．０％、０．４％、１．２％、２．０％である４種の試料を準備した。この場合、前記４種の試料のうち初期過酢酸の濃度は約０．１３重量％になるように調整した。 For this purpose, by adjusting the amount of acetylsalicylic acid charged in the second solution, the concentration of acetylsalicylic acid in the final mixture of the first solution and the second solution is 0.0%, 0.4%, Four samples of 1.2% and 2.0% were prepared. In this case, the concentration of the initial peracetic acid was adjusted to about 0.13% by weight among the four types of samples.
表１を参照すれば、アセチルサリチル酸の濃度が０％である場合、希釈された後２０日以上経過する場合、過酢酸の濃度が多少減少する傾向を示す。しかし、アセチルサリチル酸の濃度が増加する場合、過酢酸の濃度は経時的に順次増加する傾向を示すことが分かった。これは、アセチルサリチル酸の加水分解による酢酸の追加的供給によって、過酢酸の生成反応が正反応の方向に移動したことを示している。結果的に、前記第１及び第２溶液を精製水で希釈した希釈溶液で、前記希釈溶液の使用時に長期間にかける平衡の移動、または有機物及びその他の汚染物質が前記希釈溶液内の過酢酸と反応して、前記過酢酸の含有量が長時間にかけて減少する現象を、前記アセチルサリチル酸を所定の濃度以上に添加することによって効果的に防止できることを間接的に確認することができた。 Referring to Table 1, when the concentration of acetylsalicylic acid is 0%, the concentration of peracetic acid tends to decrease somewhat when 20 days or more have passed after dilution. However, it was found that when the concentration of acetylsalicylic acid increases, the concentration of peracetic acid tends to increase sequentially over time. This indicates that the peracetic acid production reaction shifted in the direction of the positive reaction due to the additional supply of acetic acid by hydrolysis of acetylsalicylic acid. As a result, a diluted solution obtained by diluting the first and second solutions with purified water, and a shift of equilibrium over a long period of time when the diluted solution is used, or organic matter and other contaminants are peracetic acid in the diluted solution. It was confirmed indirectly that the phenomenon in which the content of the peracetic acid decreases over a long period of time can be effectively prevented by adding the acetylsalicylic acid to a predetermined concentration or more.
以下、記述される実施例２ないしは４では、アセチルサリチル酸を含有した過酢酸殺菌消毒剤の希釈溶液の殺菌力の実験のために、前記第１溶液及び第２溶液を製造した後、前記二つの溶液を所定割合で混合して殺菌消毒剤原液を製造した。前記殺菌消毒剤原液を精製水で希釈することによって、前記希釈溶液中の過酢酸の濃度が５００ｐｐｍ、７５０ｐｐｍ、１０００ｐｐｍになるように調整した。 Hereinafter, in Examples 2 to 4 described below, after the first solution and the second solution were prepared for the experiment on the bactericidal activity of a diluted solution of a peracetic acid disinfectant containing acetylsalicylic acid, The solution was mixed at a predetermined ratio to produce a disinfectant stock solution. By diluting the stock solution of the disinfectant with purified water, the concentration of peracetic acid in the diluted solution was adjusted to 500 ppm, 750 ppm, and 1000 ppm.
殺菌力を測定するための微生物の種類は、黄色ブドウ球菌（ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ）、大腸菌（ｅｓｃｈｅｒｉｃｈｉａ ｃｏｌｉ；以下、Ｅ．ｃｏｌｉという）、及びカンジダ・アルビカンス（ｃａｎｄｉｄａ ａｌｂｉｃａｎｓ）の３種であり、前記使用菌株は、韓国種菌協会で分譲されたものを使用した。前記菌株はそれぞれ一般的に使われる適正培地で２４時間培養された後、４℃に保管しつつ実験に使用した。 There are three types of microorganisms for measuring the bactericidal activity: Staphylococcus aureus, Escherichia coli (hereinafter referred to as E. coli), and Candida albicans. Used the ones sold by the Korean Inoculum Association. Each of the strains was cultured in an appropriate medium generally used for 24 hours, and then used for experiments while being stored at 4 ° C.
具体的に、前記殺菌消毒剤の希釈溶液試料及びそれぞれの前記菌株の培養液０．１mLを振盪混合した後で一定時間反応させた後、前記反応液１mLを直ちに中和剤が入っている試験管に取った後、生理食塩水で適当に希釈して平板培地に塗抹した後、生存菌数を計算した。本実験で使われた中和剤としては、４．２６％リン酸二ナトリウム、３％ツインなどを使用した。 Specifically, after the sample of the diluted solution of the bactericidal disinfectant and the culture solution of each strain 0.1 mL was shaken and reacted for a certain period of time, 1 mL of the reaction solution was immediately charged with a neutralizing agent. After taking into a tube, after diluting appropriately with the physiological saline and smearing on a plate culture medium, the viable cell count was calculated. As a neutralizing agent used in this experiment, 4.26% disodium phosphate, 3% twin, and the like were used.
前記実験は、２０±１℃で行われ、反応時間は０分、５分、及び１０分に分けて実施された。
＜実施例２＞
実施例２は、黄色ブドウ球菌に対する殺菌力実験であって、第１ないし第３実験で実施された。
The experiment was performed at 20 ± 1 ° C., and the reaction time was divided into 0 minutes, 5 minutes, and 10 minutes.
<Example 2>
Example 2 is an experiment on bactericidal activity against Staphylococcus aureus, and was performed in the first to third experiments.
第１実験は、黄色ブドウ球菌の初期菌数は１．９×１０９ＣＦＵ／mLに設定し、清浄試験条件で行われ、希釈溶液内の過酢酸の濃度を５００ｐｐｍとした。コントロール試験として、アセチルサリチル酸の濃度が０％である場合、５分後の菌数は１．８×１０３ＣＦＵ／mLに減少し、１０分後には微生物が検出されていない。これに対して、前記コントロール試験と同じ条件で行われるが、アセチルサリチル酸濃度が１．２％である場合、５分後及び１０分後、いずれでも微生物は観察されていない。 In the first experiment, the initial number of Staphylococcus aureus was set to 1.9 × 10 9 CFU / mL, and the test was performed under clean test conditions. The concentration of peracetic acid in the diluted solution was 500 ppm. As a control test, when the concentration of acetylsalicylic acid was 0%, the number of bacteria after 5 minutes was reduced to 1.8 × 10 3 CFU / mL, and no microorganism was detected after 10 minutes. On the other hand, although it is performed under the same conditions as the control test, when the concentration of acetylsalicylic acid is 1.2%, no microorganism is observed after 5 minutes and after 10 minutes.
第２実験は、黄色ブドウ球菌の初期３菌数は１．９×１０９ＣＦＵ／mLに設定し、１％のイーストによる汚染条件で過酢酸の濃度を７５０ｐｐｍとした。前記第２実験では、コントロール試験なしにアセチルサリチル酸の濃度を１．２％に調整して行った結果、５分後の黄色ブドウ球菌の菌数は４．９×１０４ＣＦＵ／mLであり、１０分後には８．０×１０１ＣＦＵ／mLに減少した。 In the second experiment, the initial number of three Staphylococcus aureus bacteria was set at 1.9 × 10 9 CFU / mL, and the concentration of peracetic acid was 750 ppm under the contamination condition of 1% yeast. In the second experiment, the concentration of acetylsalicylic acid was adjusted to 1.2% without a control test. As a result, the number of Staphylococcus aureus after 5 minutes was 4.9 × 10 4 CFU / mL, After 10 minutes, it decreased to 8.0 × 10 1 CFU / mL.
第３実験は、黄色ブドウ球菌の初期菌数は１．９×１０９ＣＦＵ／mLに設定し、１％のイーストによる汚染条件で過酢酸の濃度を１０００ｐｐｍとした。コントロール試験として、アセチルサリチル酸の濃度が０％である場合、５分後の菌数は２．２×１０４ＣＦＵ／mLに減少したが、１０分後には１．１×１０４ＣＦＵ／mLであって、経時的に前記微生物の減少速度が鈍化したことが分かった。これに対して、前記コントロール試験と同じ条件で行うものの、アセチルサリチル酸を１．２％の濃度で添加した場合、５分後及び１０分後いずれも微生物が検出されていない。 In the third experiment, the initial number of Staphylococcus aureus was set to 1.9 × 10 9 CFU / mL, and the concentration of peracetic acid was 1000 ppm under the contamination condition of 1% yeast. As a control test, when the concentration of acetylsalicylic acid was 0%, the number of bacteria after 5 minutes decreased to 2.2 × 10 4 CFU / mL, but after 10 minutes, 1.1 × 10 4 CFU / mL. Thus, it was found that the rate of decrease of the microorganisms slowed over time. On the other hand, although it performs on the same conditions as the said control test, when acetylsalicylic acid is added by the density | concentration of 1.2%, microorganisms are not detected after 5 minutes and after 10 minutes.
実施例２の黄色ブドウ球菌に対する殺菌力試験での前記第１ないし第３実験の結果を比較する場合、アセチルサリチル酸をさらに含む殺菌希釈液の場合、アセチルサリチル酸を含んでいないコントロール試料に比べて一貫的に高い殺菌力を示していることが分かる。これに加えて、本発明によってアセチルサリチル酸を含む試料を、コントロール試料に比べて過酢酸の濃度を低めて前記二つの希釈溶液の５分後の殺菌力を類似して調整した場合であっても、１０分が経過した後には、アセチルサリチル酸を含有した試料は、これを含んでいない前記コントロール試料に比べて殺菌効果が大幅増加したことが分かる。
＜実施例３＞
実施例３は、Ｅ．ｃｏｌｉに対する殺菌力実験であって、Ｅ．ｃｏｌｉの初期菌数は１．９×１０９ＣＦＵ／mLに設定し、１％のイーストによる汚染条件で希釈溶液中の過酢酸濃度を５００ｐｐｍとして行われた。コントロール試験として、アセチルサリチル酸の濃度が０％である場合、５分後の菌数は１．６×１０４ＣＦＵ／mLに減少し、１０分後には微生物が検出されていない。これに対して、前記実験条件と同じ条件で行うものの、アセチルサリチル酸を１．２％の濃度で添加する場合、５分後及び１０分後のいずれでも微生物は検出されていない。
＜実施例４＞
実施例４は、カンジダ・アルビカンスに対する殺菌力実験であって、第４及び第５実験で実施された。
When comparing the results of the first to third experiments in the bactericidal activity test against Staphylococcus aureus in Example 2, the bactericidal diluted solution further containing acetylsalicylic acid is more consistent than the control sample not containing acetylsalicylic acid. It can be seen that the sterilizing power is high. In addition to this, even when the sample containing acetylsalicylic acid according to the present invention was prepared by reducing the concentration of peracetic acid as compared with the control sample and adjusting the bactericidal activity after 5 minutes of the two diluted solutions in a similar manner. After 10 minutes have passed, it can be seen that the sample containing acetylsalicylic acid has a significantly increased bactericidal effect compared to the control sample containing no acetylsalicylic acid.
<Example 3>
Example 3 is E. Bactericidal activity test against E. coli, The initial bacterial count of E. coli was set to 1.9 × 10 9 CFU / mL, and the peracetic acid concentration in the diluted solution was 500 ppm under the contamination condition of 1% yeast. As a control test, when the concentration of acetylsalicylic acid is 0%, the number of bacteria after 5 minutes is reduced to 1.6 × 10 4 CFU / mL, and no microorganism is detected after 10 minutes. On the other hand, although it is performed under the same conditions as the experimental conditions, when acetylsalicylic acid is added at a concentration of 1.2%, no microorganisms are detected after 5 minutes and after 10 minutes.
<Example 4>
Example 4 is an experiment for bactericidal activity against Candida albicans and was performed in the fourth and fifth experiments.
第４実験では、カンジダ・アルビカンスの初期菌数を４．４×１０８ＣＦＵ／mLに設定して清浄試験条件で行われ、希釈溶液内の過酢酸濃度は７５０ｐｐｍとした。コントロール試験として、アセチルサリチル酸の濃度が０％である場合、５分後の菌数は４．９×１０３ＣＦＵ／mLに減少し、１０分後には微生物が検出されていない。
これに対して、前記コントロール試験と同じ条件で行われるものの、アセチルサリチル酸を１．２％の濃度で添加する場合、５分後及び１０分後のいずれでも微生物は観察されていない。
In the fourth experiment, the initial number of Candida albicans was set to 4.4 × 10 8 CFU / mL under clean test conditions, and the peracetic acid concentration in the diluted solution was set to 750 ppm. As a control test, when the concentration of acetylsalicylic acid was 0%, the number of bacteria after 5 minutes decreased to 4.9 × 10 3 CFU / mL, and no microorganism was detected after 10 minutes.
On the other hand, although it is performed under the same conditions as in the control test, when acetylsalicylic acid is added at a concentration of 1.2%, no microorganisms are observed after 5 minutes and after 10 minutes.
第５実験では、カンジダ・アルビカンスの初期菌数を４。４×１０８ＣＦＵ／mLに設定し、清浄試験条件で行われたし希釈溶液内の過酢酸の濃度は１０００ｐｐｍとした。コントロール試験として、アセチルサリチル酸の濃度が０％である場合、５分後の菌数は１．４×１０２ＣＦＵ／mLに減少し、１０分後には微生物が検出されていない。これに対して、前記コントロール試験と同じ条件で行われるものの、アセチルサリチル酸を１．２％の濃度で添加する場合、５分後及び１０分後のいずれでも微生物は観察されていない。 In the fifth experiment, the initial number of Candida albicans was set to 4.4 × 10 8 CFU / mL, and the concentration of peracetic acid in the diluted solution was set to 1000 ppm. As a control test, when the concentration of acetylsalicylic acid was 0%, the number of bacteria after 5 minutes was reduced to 1.4 × 10 2 CFU / mL, and no microorganism was detected after 10 minutes. On the other hand, although it is performed under the same conditions as in the control test, when acetylsalicylic acid is added at a concentration of 1.2%, no microorganisms are observed after 5 minutes and after 10 minutes.
本発明による殺菌組成物は、酢酸、過酸化水素、過酢酸、及びアセチルサリチル酸を主成分とする酸性の殺菌組成物であり、前記過酢酸は、酢酸及び過酸化水素の反応により生成されて平衡状態に存在する。前記殺菌組成物は、通常精製水によって過酢酸の濃度が０．１〜０．３５％の濃度に希釈された状態で医療機器などの殺菌及び消毒に使われる。 The sterilizing composition according to the present invention is an acidic sterilizing composition mainly composed of acetic acid, hydrogen peroxide, peracetic acid, and acetylsalicylic acid, and the peracetic acid is produced by the reaction of acetic acid and hydrogen peroxide to achieve equilibrium. Exists in the state. The sterilizing composition is usually used for sterilization and disinfection of medical devices in a state in which the concentration of peracetic acid is diluted with purified water to a concentration of 0.1 to 0.35%.
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| CN102578144B (en) * | 2011-01-13 | 2014-04-02 | 北京康福乐科技有限公司 | Product and method for generating peracetic acid |
| CN102964285A (en) * | 2012-11-28 | 2013-03-13 | 北京红辉力上科技有限公司 | Peroxyacetic acid generator and preparation method thereof |
| CN104782671A (en) * | 2015-03-25 | 2015-07-22 | 彭新国 | Bacteriostatic disinfecting disinfectant for laboratory department and preparation method for bacteriostatic disinfecting disinfectant |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH02193905A (en) * | 1988-11-23 | 1990-07-31 | L'air Liquide | Hygienic agent for hemodialysis |
| JPH02502532A (en) * | 1987-05-14 | 1990-08-16 | ミンテック コーポレーション | Stable, transportable, peroxy-containing microbicide |
| JPH02295564A (en) * | 1989-04-24 | 1990-12-06 | Steris Corp | Antibacterial powder composite |
| JPH09500630A (en) * | 1993-07-12 | 1997-01-21 | ステリス コーポレイション | Cold sterilant with extended active life |
| US20040002616A1 (en) * | 2002-06-03 | 2004-01-01 | Andrea Preto | Method of preparing organic peroxyacids |
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| KR100271813B1 (en) * | 1998-09-28 | 2000-11-15 | 구본준 | A method of crystallizing a silicon layer, a tft and a method thereof using the same |
| DE69831816T2 (en) * | 1998-10-19 | 2006-07-06 | Saraya Co., Ltd. | BACTERICIDES DISINFECTING PERACIC ACID COMPOSITION |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02502532A (en) * | 1987-05-14 | 1990-08-16 | ミンテック コーポレーション | Stable, transportable, peroxy-containing microbicide |
| JPH02193905A (en) * | 1988-11-23 | 1990-07-31 | L'air Liquide | Hygienic agent for hemodialysis |
| JPH02295564A (en) * | 1989-04-24 | 1990-12-06 | Steris Corp | Antibacterial powder composite |
| JPH09500630A (en) * | 1993-07-12 | 1997-01-21 | ステリス コーポレイション | Cold sterilant with extended active life |
| US20040002616A1 (en) * | 2002-06-03 | 2004-01-01 | Andrea Preto | Method of preparing organic peroxyacids |
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| WO2009057835A1 (en) | 2009-05-07 |
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