JP2009532052A - プロテアーゼ検出製品 - Google Patents
プロテアーゼ検出製品 Download PDFInfo
- Publication number
- JP2009532052A JP2009532052A JP2009503659A JP2009503659A JP2009532052A JP 2009532052 A JP2009532052 A JP 2009532052A JP 2009503659 A JP2009503659 A JP 2009503659A JP 2009503659 A JP2009503659 A JP 2009503659A JP 2009532052 A JP2009532052 A JP 2009532052A
- Authority
- JP
- Japan
- Prior art keywords
- protease
- capture
- sample
- flow path
- component
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091005804 Peptidases Proteins 0.000 title claims abstract description 132
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title claims abstract description 126
- 239000004365 Protease Substances 0.000 title claims abstract description 107
- 238000001514 detection method Methods 0.000 title claims abstract description 43
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 91
- 239000007788 liquid Substances 0.000 claims abstract description 58
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 29
- 239000010931 gold Substances 0.000 claims description 29
- 229910052737 gold Inorganic materials 0.000 claims description 29
- 230000004888 barrier function Effects 0.000 claims description 24
- 239000002245 particle Substances 0.000 claims description 15
- 238000011144 upstream manufacturing Methods 0.000 claims description 14
- 229960002685 biotin Drugs 0.000 claims description 11
- 239000011616 biotin Substances 0.000 claims description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 10
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims description 9
- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims description 9
- 108010090804 Streptavidin Proteins 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 229920002678 cellulose Polymers 0.000 claims description 7
- 239000001913 cellulose Substances 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- OMDQUFIYNPYJFM-XKDAHURESA-N (2r,3r,4s,5r,6s)-2-(hydroxymethyl)-6-[[(2r,3s,4r,5s,6r)-4,5,6-trihydroxy-3-[(2s,3s,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]methoxy]oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@H](O)[C@H](O)O1 OMDQUFIYNPYJFM-XKDAHURESA-N 0.000 claims description 5
- 229920000926 Galactomannan Polymers 0.000 claims description 5
- 235000020958 biotin Nutrition 0.000 claims description 5
- 229920002000 Xyloglucan Polymers 0.000 claims description 4
- 239000011534 wash buffer Substances 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 238000002372 labelling Methods 0.000 abstract description 10
- 239000003550 marker Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 29
- 238000003776 cleavage reaction Methods 0.000 description 15
- 230000007017 scission Effects 0.000 description 15
- 239000000020 Nitrocellulose Substances 0.000 description 14
- 229920001220 nitrocellulos Polymers 0.000 description 14
- 239000000203 mixture Substances 0.000 description 13
- 239000004372 Polyvinyl alcohol Substances 0.000 description 12
- 230000002745 absorbent Effects 0.000 description 12
- 239000002250 absorbent Substances 0.000 description 12
- 229920002451 polyvinyl alcohol Polymers 0.000 description 12
- 238000003556 assay Methods 0.000 description 9
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 8
- 238000003018 immunoassay Methods 0.000 description 8
- 239000002953 phosphate buffered saline Substances 0.000 description 8
- 102000035195 Peptidases Human genes 0.000 description 6
- 102000004142 Trypsin Human genes 0.000 description 6
- 108090000631 Trypsin Proteins 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 102000004196 processed proteins & peptides Human genes 0.000 description 6
- 239000012588 trypsin Substances 0.000 description 6
- 238000009825 accumulation Methods 0.000 description 5
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 3
- 238000012800 visualization Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 102000004856 Lectins Human genes 0.000 description 2
- 108090001090 Lectins Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 150000002009 diols Chemical class 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 229940027941 immunoglobulin g Drugs 0.000 description 2
- 239000002523 lectin Substances 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 230000007030 peptide scission Effects 0.000 description 2
- HXITXNWTGFUOAU-UHFFFAOYSA-N phenylboronic acid Chemical group OB(O)C1=CC=CC=C1 HXITXNWTGFUOAU-UHFFFAOYSA-N 0.000 description 2
- 238000009597 pregnancy test Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 1
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 241000208829 Sambucus Species 0.000 description 1
- 235000018735 Sambucus canadensis Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 235000007123 blue elder Nutrition 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000007124 elderberry Nutrition 0.000 description 1
- 235000008995 european elder Nutrition 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
−液体流路を提供する媒体(medium);
−第一の位置で液体流路上に配置することができる切断可能コンポーネント(要素ないし成分、cleavable component)であって、前記切断可能コンポーネントはプロテアーゼ感受性リンカーペプチドを介して遊離可能エレメントに連結されたベースエレメントを含む前記切断可能コンポーネント;
−前記遊離可能エレメントは標識結合構造を含み、標識は前記標識結合構造と結合することができる標識;
−前記液体流路の第一の位置の下流に配置された、前記遊離可能エレメントと結合することができる、捕捉コンポーネント(要素ないし成分、capture component);及び
−前記無傷の切断可能コンポーネントを固定するための固定化手段。
−液体流路を提供する媒体;
−液体流路上に配置された標識されたコンポーネント(要素ないし成分)(labeled component)、プロテアーゼ感受性ペプチド結合を介してエレメント遊離物(release of an element)と連結されたベースエレメントを含む切断可能コンポーネント(要素ないし成分)、該遊離可能エレメント(ないし成分、The releasable element)は標識を含む;
−標識コンポーネントの下流に配置された捕捉コンポーネント(要素ないし成分)、該補足コンポーネントは液体流路上で該遊離可能エレメントと結合することができる前記捕捉コンポーネント;及び
−無傷の切断可能コンポーネントを固定するための固定化手段。
ストレプトアビジンの試験線1及び抗マウス抗体の試験線2を含むイムノアッセイ試験細片を用いて、固定ペプチドのプロテアーゼ切断を示すべく以下のアッセイを実施した。切断可能ペプチド(フルオレセイン-Val-Arg-Gly-[PEG]20-ビオチン(a))をストレプトアビジン/ビオチン結合により細片に固定し、その後、細片にトリプシン含有溶液(これはArg残基のC-末端結合で前記ペプチドを切断することができる)を流すことにより切断した:
a)4μLのペプチド(0.65μg/mL)(フルオレセイン-Val-Arg-Gly-[PEG]20-ビオチン)を10μLのウシ血清アルブミン(200mg/mLのBSA)及び20μLのリン酸緩衝食塩水(PBS, pH7.4)と混合し、支持台上に垂直に保持した6枚の細片の基部に添加し、各細片の上方へクロマトグラフさせた。
ストレプトアビジンの試験線1及び抗マウス抗体の試験線2を含むイムノアッセイ試験細片を用い、サンプル中のトリプシン活性の検出のために蛍光を利用して切断可能ペプチド(フルオレセイン-Val-Arg-Gly-[PEG]20-ビオチン)により以下のアッセイを実施した:
a)4μLの体積のペプチド(50μg/mL)(フルオレセイン-Val-Arg-Gly-[PEG]20-ビオチン)を4μLの体積のトリプシン標準物(0、10、100及び1000μg/mL)と混合し、室温で5分間インキュベートした。
ストレプトアビジンの試験線1及び抗マウス抗体の試験線2を含むイムノアッセイ試験細片を用いて試験細片のサンプルパッドに乾燥させて供給した切断可能ペプチド(フルオレセイン-Val-Arg-Gly-[PEG]20-ビオチン)のトリプシン切断を示すべく以下のアッセイを実施した:
a)以下の溶液を調製し、続いて60*32mmの長さのサンプルパッドに染み込ませ、さらに室温で3時間乾燥させた:200mg/mLのBSAを1mL+1%のトリトンX-100を500μL+蒸留水2mL+2gのシュクロース+50μg/mLの切断可能ペプチドを500μL。
Claims (18)
- 以下を含む、サンプル中のプロテアーゼ酵素を検出するためのプロテアーゼ検出製品:
−液体流路を提供する媒体;
−第一の位置で液体流路上に配置することができる切断可能コンポーネント(要素ないし成分)であって、前記切断可能コンポーネントはプロテアーゼ感受性リンカーペプチドを介して遊離可能エレメントに連結されたベースエレメントを含み、前記遊離可能エレメントは標識結合構造を含む、前記切断可能コンポーネント;
−前記標識結合構造と結合することができる標識;
−前記液体流路の第一の位置の下流に配置され、前記遊離可能エレメントと結合することができる、捕捉コンポーネント(要素ないし成分);及び
−完全な切断可能コンポーネントを固定するための固定化手段。 - 前記固定化手段がベースエレメントと液体流路上の構造との間にアタッチメントを含む、請求項1に記載のプロテアーゼ検出製品。
- 前記固定化手段が、第一の位置の下流に配置された更に別の捕捉コンポーネントを含み、この更に別の捕捉コンポーネントは、プロテアーゼ感受性リンカーペプチドに亘るアミノ酸配列;プロテアーゼ酵素活性によって破壊されるリンカーペプチドの構造性エピトープ;又は前記リンカーペプチドが配列と標識結合構造との間に存在するように配置した前記配列と結合することができる、請求項1に記載のプロテアーゼ検出製品。
- 前記捕捉コンポーネントが液体流路内の固定化手段の上流に存在する、請求項1から3のいずれか1項に記載のプロテアーゼ検出製品。
- 前記捕捉コンポーネントが液体流路内の固定化手段の下流に存在する、請求項1から3のいずれか1項に記載のプロテアーゼ検出製品。
- さらに、液体流路上の1つの又は複数の捕捉コンポーネントの上流に配置されたプロテアーゼ阻害剤を含む、請求項1から5のいずれか1項に記載のプロテアーゼ検出製品。
- さらに、液体流路上に分解性又は破壊性のバリアーを含む、請求項1から6のいずれか1項に記載のプロテアーゼ検出製品。
- 前記分解性又は破壊性バリアーがリップを含み、前記リップは液体流路の外側に向かって伸び、物質が液体流路を流れる際に前記分解性又は破壊性のバリアー周辺での物質の通過を妨げることを目的とする、請求項7に記載のプロテアーゼ検出製品。
- さらに、切断可能コンポーネント(要素ないし成分)及びサンプルを混合させるために混合ゾーンを含み、前記分解性又は破壊性バリアーは混合ゾーンの下流及び1つの又は複数の捕捉コンポーネントの上流に配置される、請求項7又は8に記載のプロテアーゼ検出製品。
- 捕捉コンポーネント又は捕捉コンポーネントの各々が、結合対の片方を含む、請求項1から9のいずれか1項に記載のプロテアーゼ検出製品。
- 捕捉コンポーネント又は捕捉コンポーネントの各々が、抗体又はその抗原結合フラグメントを含む、請求項10に記載のプロテアーゼ検出製品。
- 捕捉コンポーネント又は捕捉コンポーネントの各々がセルロースを含み、前記遊離可能エレメントがさらに、セルロースが結合することができる結合半部(モイエティ)を含む、請求項10に記載のプロテアーゼ検出製品。
- 前記結合半部がガラクトマンナン又はキシログルカンである、請求項12に記載のプロテアーゼ検出製品。
- 捕捉コンポーネント又は捕捉コンポーネントの各々が、ビオチン又はストレプトアビジンを含む、請求項10に記載のプロテアーゼ検出製品。
- 標識が金粒子又は発蛍光団である、請求項1から14のいずれか1項に記載のプロテアーゼ検出製品。
- 請求項1から15のいずれか1項に記載のプロテアーゼ検出製品を用いて、サンプル中のプロテアーゼ酵素を検出する方法であって、前記方法は、
第一の位置で切断可能コンポーネント(要素ないし成分)を提供する工程;
液体流路の上流端でサンプルを提供し、サンプルが液体流路に沿って流れるようにする工程;及び
捕捉コンポーネント(要素ないし成分)及び/又は固定化手段で標識の存在を決定する工程を含む、
前記プロテアーゼ酵素を検出する方法。 - さらに、液体流路の上流端に別個に標識を添加し、この標識が液体流路に沿って流れるようにする工程を含む、請求項16に記載の方法。
- さらに、サンプルを液体流路に適用する工程の後で、液体流路の上流端に洗浄緩衝液を適用する工程を含む、請求項16又は17に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0607070.0 | 2006-04-07 | ||
GB0607070A GB2437311A (en) | 2006-04-07 | 2006-04-07 | A protease detection product |
PCT/GB2007/001291 WO2007128980A1 (en) | 2006-04-07 | 2007-04-05 | A protease detection product |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2009532052A true JP2009532052A (ja) | 2009-09-10 |
JP2009532052A5 JP2009532052A5 (ja) | 2010-05-13 |
JP5133976B2 JP5133976B2 (ja) | 2013-01-30 |
Family
ID=36539569
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009503659A Expired - Fee Related JP5133976B2 (ja) | 2006-04-07 | 2007-04-05 | プロテアーゼ検出製品 |
Country Status (11)
Country | Link |
---|---|
US (1) | US20090203059A1 (ja) |
EP (1) | EP2004845B1 (ja) |
JP (1) | JP5133976B2 (ja) |
AT (1) | ATE523600T1 (ja) |
AU (1) | AU2007246914B2 (ja) |
CA (1) | CA2648712A1 (ja) |
DK (1) | DK2004845T3 (ja) |
ES (1) | ES2370437T3 (ja) |
GB (1) | GB2437311A (ja) |
PL (1) | PL2004845T3 (ja) |
WO (1) | WO2007128980A1 (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016535282A (ja) * | 2013-10-23 | 2016-11-10 | モロジック・リミテッドMologic Limited | 酵素の切断活性の検出 |
JP2018205273A (ja) * | 2017-06-09 | 2018-12-27 | 東洋紡株式会社 | イムノクロマト試験片およびキットおよび測定方法 |
JP2019526326A (ja) * | 2016-08-17 | 2019-09-19 | マイクロアレイ・リミテッドMicroarray Limited | 創傷の状態の決定 |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7906276B2 (en) | 2004-06-30 | 2011-03-15 | Kimberly-Clark Worldwide, Inc. | Enzymatic detection techniques |
US7521226B2 (en) | 2004-06-30 | 2009-04-21 | Kimberly-Clark Worldwide, Inc. | One-step enzymatic and amine detection technique |
US7504235B2 (en) | 2005-08-31 | 2009-03-17 | Kimberly-Clark Worldwide, Inc. | Enzyme detection technique |
GB2435511A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | Protease detection |
GB2435510A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | Enzyme detection product and methods |
GB2435512A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | A binding assay and assay device |
US8758989B2 (en) | 2006-04-06 | 2014-06-24 | Kimberly-Clark Worldwide, Inc. | Enzymatic detection techniques |
US7897360B2 (en) | 2006-12-15 | 2011-03-01 | Kimberly-Clark Worldwide, Inc. | Enzyme detection techniques |
GB2452076A (en) | 2007-08-23 | 2009-02-25 | Mologic Ltd | Detection of enzymes by detecting binding of substrate recognition molecules to modified substrates |
CA2715033C (en) * | 2008-03-14 | 2014-11-25 | Allergan, Inc. | Immuno-based botulinum toxin serotype a activity assays |
WO2009125510A1 (ja) | 2008-04-11 | 2009-10-15 | 三菱電機株式会社 | 加速度センサ |
US8815609B2 (en) | 2008-05-20 | 2014-08-26 | Rapid Pathogen Screening, Inc. | Multiplanar lateral flow assay with diverting zone |
US20130196310A1 (en) | 2008-05-20 | 2013-08-01 | Rapid Pathogen Screening, Inc. | Method and Device for Combined Detection of Viral and Bacterial Infections |
US8962260B2 (en) | 2008-05-20 | 2015-02-24 | Rapid Pathogen Screening, Inc. | Method and device for combined detection of viral and bacterial infections |
GB0904080D0 (en) | 2009-03-09 | 2009-04-22 | Mologic Ltd | Imaging method |
US20120009591A1 (en) * | 2009-03-20 | 2012-01-12 | Ewa Pol | Method for detection of an enzyme-substrate reaction |
GB201116523D0 (en) | 2011-09-23 | 2011-11-09 | Systagenix Wound Man Ip Co Bv | Wound prognosis |
WO2012104620A2 (en) * | 2011-01-31 | 2012-08-09 | Systagenix Wound Management Ip Co. B.V. | Wound prognosis |
WO2013016114A2 (en) * | 2011-07-22 | 2013-01-31 | Rapid Pathogen Screening, Inc. | Enzymatic cleavage based lateral flow assays |
GB201206976D0 (en) * | 2012-04-20 | 2012-06-06 | Mologic Ltd | An enzyme detection device |
GB201206977D0 (en) | 2012-04-20 | 2012-06-06 | Mologic Ltd | An enzyme detection device |
US20160282343A1 (en) * | 2012-08-15 | 2016-09-29 | Immunolab LLC | Quantitative lateral flow assay strips for quantitative analysis of an analyte, kits containing such strips and methods of manufacture and use of same |
US10808287B2 (en) | 2015-10-23 | 2020-10-20 | Rapid Pathogen Screening, Inc. | Methods and devices for accurate diagnosis of infections |
GB201605110D0 (en) | 2016-03-24 | 2016-05-11 | Mologic Ltd | Detecting sepsis |
GB201902458D0 (en) | 2019-02-22 | 2019-04-10 | Mologic Ltd | Treatment stratification for an excerbation of inflamation |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002202308A (ja) * | 2000-12-27 | 2002-07-19 | Matsushita Electric Ind Co Ltd | 免疫クロマトデバイス |
WO2006006961A1 (en) * | 2004-06-30 | 2006-01-19 | Kimberly-Clark Worldwide, Inc. | Enzymatic detection techniques |
Family Cites Families (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1006557B (it) * | 1971-09-08 | 1976-10-20 | Bagshawe Kenneth Dawson | Cella di reazione particolarmente utile in prove di radioimmunita |
US4055394A (en) * | 1976-10-18 | 1977-10-25 | Akzona Incorporated | Diagnostic test card |
CA1095819A (en) * | 1977-01-14 | 1981-02-17 | Eastman Kodak Company | Element for analysis of liquids |
US4178153A (en) * | 1977-11-21 | 1979-12-11 | Damon Corporation | Method and apparatus for chemical spot test analysis |
US4258001A (en) * | 1978-12-27 | 1981-03-24 | Eastman Kodak Company | Element, structure and method for the analysis or transport of liquids |
JPS568549A (en) * | 1979-07-02 | 1981-01-28 | Fuji Photo Film Co Ltd | Multilayer chemical analyzing material |
JPS5767860A (en) * | 1980-10-15 | 1982-04-24 | Fuji Photo Film Co Ltd | Material for multilayer analysis |
DE3247608A1 (de) * | 1982-12-23 | 1984-07-05 | Boehringer Mannheim Gmbh, 6800 Mannheim | Teststreifen |
US4851356A (en) * | 1983-05-06 | 1989-07-25 | The Trustees Of Columbia University In The City Of New York | Immunoassay for human chorionic gonadotropin |
US4522923A (en) * | 1983-10-03 | 1985-06-11 | Genetic Diagnostics Corporation | Self-contained assay method and kit |
US4703017C1 (en) * | 1984-02-14 | 2001-12-04 | Becton Dickinson Co | Solid phase assay with visual readout |
US4632901A (en) * | 1984-05-11 | 1986-12-30 | Hybritech Incorporated | Method and apparatus for immunoassays |
US5879881A (en) * | 1985-04-04 | 1999-03-09 | Hybritech, Incorporated | Solid phase system for use in ligand-receptor assays |
US4689240A (en) * | 1985-06-20 | 1987-08-25 | Miles Laboratories, Inc. | Method for forming volume independent test device |
US5236826A (en) * | 1985-12-10 | 1993-08-17 | Murex Corporation | Immunoassay for the detection or quantitation of an analyte |
JPS63305251A (ja) * | 1987-06-05 | 1988-12-13 | Dai Ichi Pure Chem Co Ltd | ラテツクス凝集反応を利用する免疫学的測定方法 |
FR2621393B1 (fr) * | 1987-10-05 | 1991-12-13 | Toledano Jacques | Dispositif de detection immunoenzymatique de substances a partir d'une goutte de sang ou de liquide provenant d'un quelconque milieu biologique |
DE3814370A1 (de) * | 1988-04-28 | 1989-11-09 | Boehringer Mannheim Gmbh | Testtraeger fuer die analyse einer probenfluessigkeit, verfahren zur durchfuehrung einer solchen analyse und herstellungsverfahren |
US6352862B1 (en) * | 1989-02-17 | 2002-03-05 | Unilever Patent Holdings B.V. | Analytical test device for imuno assays and methods of using same |
US5064770A (en) * | 1989-08-01 | 1991-11-12 | Wisconsin Alumni Research Foundation | Assay for 1,25-dihydroxy vitamin d receptor protein |
US5185249A (en) * | 1990-04-04 | 1993-02-09 | Eastman Kodak Company | Dry analytical element for assaying salicylate |
US5171662A (en) * | 1990-09-13 | 1992-12-15 | The Upjohn Company | Method of detecting HIV protease activity |
ATE153138T1 (de) * | 1990-09-26 | 1997-05-15 | Akers Lab Inc | Verbessertes bestimmungsverfahren für liganden |
AU9039691A (en) * | 1990-11-14 | 1992-06-11 | Southern Research Institute | Rapid diagnostic device and kit |
EP0566695B1 (en) * | 1991-01-11 | 1999-06-02 | Quidel Corporation | A one-step lateral flow assay and nonbibulous support used therein |
GB9119102D0 (en) * | 1991-09-07 | 1991-10-23 | Biosyn Ltd | Bioimmunassay |
DE4132743A1 (de) * | 1991-10-02 | 1993-04-08 | Boehringer Mannheim Gmbh | Testtraeger fuer die analyse von fluessigkeiten |
US5296192A (en) * | 1992-04-03 | 1994-03-22 | Home Diagnostics, Inc. | Diagnostic test strip |
US5601986A (en) * | 1994-07-14 | 1997-02-11 | Amgen Inc. | Assays and devices for the detection of extrahepatic biliary atresia |
US6686170B1 (en) * | 1994-08-17 | 2004-02-03 | Abbott Laboratories | Assay devices with mobile control reagents |
AUPM769394A0 (en) * | 1994-08-25 | 1994-09-15 | Commonwealth Scientific And Industrial Research Organisation | Assay for the detection of proteases |
US5755231A (en) * | 1995-05-17 | 1998-05-26 | Plus Bio, Inc. | Test strip including integral specimen flow retarding structure |
US5741659A (en) * | 1996-01-04 | 1998-04-21 | The United States Of America As Represented By The Secretary Of The Navy | Rapid microbial protease assay |
US6096563A (en) * | 1996-03-29 | 2000-08-01 | Strategic Diagnostics Inc. | Dual particle immunoassay method and kit |
US5879951A (en) * | 1997-01-29 | 1999-03-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing unidirectional flow |
US7312302B2 (en) * | 1997-02-20 | 2007-12-25 | Oncolmmunin, Inc. | Compositions for the detection of enzyme activity in biological samples and methods of use thereof |
US6040195A (en) * | 1997-06-10 | 2000-03-21 | Home Diagnostics, Inc. | Diagnostic sanitary test strip |
US6316205B1 (en) * | 2000-01-28 | 2001-11-13 | Genelabs Diagnostics Pte Ltd. | Assay devices and methods of analyte detection |
US6750034B1 (en) * | 2000-06-30 | 2004-06-15 | Ortho-Mcneil Pharmaceutical, Inc. | DNA encoding human serine protease D-G |
KR100425525B1 (ko) * | 2000-11-21 | 2004-03-30 | 재단법인서울대학교산학협력재단 | 조산과 태아감염 및 태아손상을 산전 진단하기 위한진단시약 및 진단키트 |
FR2817961B1 (fr) * | 2000-12-08 | 2003-08-01 | Diagast | Barriere de separation temporaire, recipient la comprenant et procede de mise en oeuvre d'un test dans ce recipient |
CA2456590A1 (en) * | 2001-08-07 | 2003-02-20 | Merck & Co., Inc. | Biological assay detection method |
WO2003023360A2 (en) * | 2001-09-10 | 2003-03-20 | Meso Scale Technologies, Llc | Methods and apparatus for conducting multiple measurements on a sample |
EP1472238B1 (en) * | 2002-02-01 | 2012-07-04 | Promega Corporation | Bioluminescent protease assay |
US7041453B2 (en) * | 2002-08-22 | 2006-05-09 | Bioarray Solutions Ltd. | Molecular constructs and methods of use for detection of biochemical reactions |
CA2506197A1 (en) * | 2002-11-22 | 2004-06-10 | Marligen Biosciences, Inc. | Detection of protease enzymes |
GB0316075D0 (en) * | 2003-07-09 | 2003-08-13 | Molecular Sensing Plc | Protease detection assay |
GB0317680D0 (en) * | 2003-07-29 | 2003-09-03 | Univ Edinburgh | Protease assay |
US7504235B2 (en) * | 2005-08-31 | 2009-03-17 | Kimberly-Clark Worldwide, Inc. | Enzyme detection technique |
US7279136B2 (en) * | 2005-12-13 | 2007-10-09 | Takeuchi James M | Metering technique for lateral flow assay devices |
GB2433260A (en) * | 2005-12-16 | 2007-06-20 | Mologic Ltd | A selectable decarboxylase marker |
GB2435511A (en) * | 2006-02-23 | 2007-08-29 | Mologic Ltd | Protease detection |
GB2435510A (en) * | 2006-02-23 | 2007-08-29 | Mologic Ltd | Enzyme detection product and methods |
GB2435512A (en) * | 2006-02-23 | 2007-08-29 | Mologic Ltd | A binding assay and assay device |
GB2452076A (en) * | 2007-08-23 | 2009-02-25 | Mologic Ltd | Detection of enzymes by detecting binding of substrate recognition molecules to modified substrates |
GB0904080D0 (en) * | 2009-03-09 | 2009-04-22 | Mologic Ltd | Imaging method |
-
2006
- 2006-04-07 GB GB0607070A patent/GB2437311A/en not_active Withdrawn
-
2007
- 2007-04-05 JP JP2009503659A patent/JP5133976B2/ja not_active Expired - Fee Related
- 2007-04-05 AU AU2007246914A patent/AU2007246914B2/en not_active Ceased
- 2007-04-05 PL PL07732335T patent/PL2004845T3/pl unknown
- 2007-04-05 AT AT07732335T patent/ATE523600T1/de not_active IP Right Cessation
- 2007-04-05 WO PCT/GB2007/001291 patent/WO2007128980A1/en active Application Filing
- 2007-04-05 DK DK07732335.0T patent/DK2004845T3/da active
- 2007-04-05 ES ES07732335T patent/ES2370437T3/es active Active
- 2007-04-05 CA CA002648712A patent/CA2648712A1/en not_active Abandoned
- 2007-04-05 US US12/296,413 patent/US20090203059A1/en not_active Abandoned
- 2007-04-05 EP EP07732335A patent/EP2004845B1/en not_active Not-in-force
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002202308A (ja) * | 2000-12-27 | 2002-07-19 | Matsushita Electric Ind Co Ltd | 免疫クロマトデバイス |
WO2006006961A1 (en) * | 2004-06-30 | 2006-01-19 | Kimberly-Clark Worldwide, Inc. | Enzymatic detection techniques |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016535282A (ja) * | 2013-10-23 | 2016-11-10 | モロジック・リミテッドMologic Limited | 酵素の切断活性の検出 |
JP2019526326A (ja) * | 2016-08-17 | 2019-09-19 | マイクロアレイ・リミテッドMicroarray Limited | 創傷の状態の決定 |
JP7089294B2 (ja) | 2016-08-17 | 2022-06-22 | マイクロアレイ・リミテッド | 創傷の状態の決定 |
JP2018205273A (ja) * | 2017-06-09 | 2018-12-27 | 東洋紡株式会社 | イムノクロマト試験片およびキットおよび測定方法 |
Also Published As
Publication number | Publication date |
---|---|
GB2437311A (en) | 2007-10-24 |
US20090203059A1 (en) | 2009-08-13 |
ES2370437T3 (es) | 2011-12-15 |
EP2004845B1 (en) | 2011-09-07 |
EP2004845A1 (en) | 2008-12-24 |
AU2007246914B2 (en) | 2013-03-14 |
CA2648712A1 (en) | 2007-11-15 |
AU2007246914A1 (en) | 2007-11-15 |
DK2004845T3 (da) | 2011-11-21 |
JP5133976B2 (ja) | 2013-01-30 |
GB0607070D0 (en) | 2006-05-17 |
PL2004845T3 (pl) | 2012-02-29 |
ATE523600T1 (de) | 2011-09-15 |
WO2007128980A1 (en) | 2007-11-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5133976B2 (ja) | プロテアーゼ検出製品 | |
CN1902489B (zh) | 对样品中分析物的诊断试验 | |
US6998246B2 (en) | Method for immobilizing conjugates in diagnostic tests | |
AU2008221293B2 (en) | Diagnostic detection device | |
JP3066540B2 (ja) | ボード上に陰性および陽性対照を有する分析試験装置 | |
US6472226B1 (en) | Assay using porosity-reduction to inhibit migration | |
US7935497B2 (en) | Protease detection | |
US20090191648A1 (en) | Method and device to detect the presence of analytes in a sample | |
US20080138842A1 (en) | Indirect lateral flow sandwich assay | |
CN111164095A (zh) | 用于改进分析物检测的测定方法 | |
EP2839288B1 (en) | An enzyme detection device | |
WO2007105680A1 (ja) | 免疫測定用吸収パッド、免疫測定用ストリップ及び免疫測定装置 | |
US20130164858A1 (en) | Diagnostic detection device | |
EP2839022B1 (en) | An enzyme detection device | |
JP2021531763A (ja) | 細菌性膣炎の診断 | |
JPH1164336A (ja) | 免疫クロマトグラフィーによる分析対象物の検出方法 | |
JP2008051806A (ja) | 免疫測定装置用の対照系としてのウシアルブミンp−アミノフェニルN−アセチルβ−Dグルコサミニドの使用 | |
CN112912730A (zh) | 用于差分同种型检测的横向流动测定 | |
US6686167B2 (en) | Test device for detecting semen and method of use | |
JP2010101673A (ja) | イムノクロマトグラフ法による高感度測定キット | |
JPH09133681A (ja) | 酵素免疫アッセイ装置及び方法 | |
JP2005003569A (ja) | 検出装置及び検出方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100324 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20100324 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20110811 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110823 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20111124 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20120313 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20121106 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20121108 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20151116 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
LAPS | Cancellation because of no payment of annual fees |