JP2009515515A5 - - Google Patents

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Publication number
JP2009515515A5
JP2009515515A5 JP2008539502A JP2008539502A JP2009515515A5 JP 2009515515 A5 JP2009515515 A5 JP 2009515515A5 JP 2008539502 A JP2008539502 A JP 2008539502A JP 2008539502 A JP2008539502 A JP 2008539502A JP 2009515515 A5 JP2009515515 A5 JP 2009515515A5
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JP
Japan
Prior art keywords
cell
mek inhibitor
mammalian
cells
treating
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Pending
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JP2008539502A
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Japanese (ja)
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JP2009515515A (en
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Priority claimed from GB0523039A external-priority patent/GB0523039D0/en
Priority claimed from GB0605998A external-priority patent/GB0605998D0/en
Application filed filed Critical
Priority claimed from PCT/GB2006/004218 external-priority patent/WO2007054720A1/en
Publication of JP2009515515A publication Critical patent/JP2009515515A/en
Publication of JP2009515515A5 publication Critical patent/JP2009515515A5/ja
Pending legal-status Critical Current

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細胞をＭＥＫ阻害剤で処理することを含む、体細胞を再プログラム化する方法。   A method of reprogramming somatic cells comprising treating the cells with a MEK inhibitor. 体細胞が哺乳動物細胞である、請求項１記載の方法。   The method of claim 1, wherein the somatic cell is a mammalian cell. 哺乳動物細胞がマウスまたはヒトのものである、請求項２記載の方法。   The method of claim 2, wherein the mammalian cell is mouse or human. 細胞がインビトロで再プログラム化される、請求項１記載の方法。   The method of claim 1, wherein the cell is reprogrammed in vitro. 細胞が神経幹細胞である、請求項１記載の方法。   The method of claim 1, wherein the cell is a neural stem cell. ＭＥＫ阻害剤がＭＥＫ１阻害剤である、請求項１記載の方法。   The method of claim 1, wherein the MEK inhibitor is a MEK1 inhibitor. ＭＥＫ阻害剤がＰＤ１８４３５４である、請求項１記載の方法。   The method of claim 1, wherein the MEK inhibitor is PD184354. 細胞がＮａｎｏｇを内在的に発現する、請求項１記載の方法。   The method of claim 1, wherein the cell endogenously expresses Nanog. Ｎａｎｏｇを内在的に発現する細胞におけるＮａｎｏｇの発現を上方調節する方法であって、細胞をＭＥＫ阻害剤で処理することを含む方法。   A method of up-regulating Nanog expression in a cell that endogenously expresses Nanog, comprising treating the cell with a MEK inhibitor. 細胞が哺乳動物である、請求項９記載の方法。   The method of claim 9, wherein the cell is a mammal. 哺乳動物細胞がマウスまたはヒトのものである、請求項１０記載の方法。   11. The method of claim 10, wherein the mammalian cell is mouse or human. 細胞が神経幹細胞である、請求項９記載の方法。   The method of claim 9, wherein the cell is a neural stem cell. ＭＥＫ阻害剤がＰＤ１８４３５２である、請求項９記載の方法。   10. The method of claim 9, wherein the MEK inhibitor is PD184352. 哺乳動物の体細胞の核を哺乳動物のレシピエント細胞に移植して核移植細胞を形成させ、次いで前記核移植細胞をＭＥＫ阻害剤で処理することを含む、核移植の方法。   A method of nuclear transfer, comprising transplanting a nucleus of a mammalian somatic cell into a mammalian recipient cell to form a nuclear transplant cell, and then treating the nuclear transplant cell with a MEK inhibitor. レシピエント細胞が卵母細胞である、請求項１４記載の方法。   15. The method of claim 14, wherein the recipient cell is an oocyte. レシピエント細胞が除核された卵母細胞である、請求項１４記載の方法。   15. The method of claim 14, wherein the recipient cell is an enucleated oocyte. 核移植細胞から生きた非ヒト動物を得ることをさらに含む、請求項１４記載の方法。   15. The method of claim 14, further comprising obtaining a live non-human animal from the nuclear transfer cells. 核移植細胞から多能性幹細胞の培養物を得ることをさらに含む、請求項１４記載の方法。   15. The method of claim 14, further comprising obtaining a culture of pluripotent stem cells from the nuclear transfer cells.
JP2008539502A 2005-11-11 2006-11-13 Cell reprogramming and genetic modification Pending JP2009515515A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0523039A GB0523039D0 (en) 2005-11-11 2005-11-11 Reprogramming and genetic modification of cells
GB0605998A GB0605998D0 (en) 2006-03-24 2006-03-24 Reprogramming And Genetic Modification Of Cells
PCT/GB2006/004218 WO2007054720A1 (en) 2005-11-11 2006-11-13 Reprogramming and genetic modification of cells

Publications (2)

Publication Number Publication Date
JP2009515515A JP2009515515A (en) 2009-04-16
JP2009515515A5 true JP2009515515A5 (en) 2010-01-07

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Application Number Title Priority Date Filing Date
JP2008539502A Pending JP2009515515A (en) 2005-11-11 2006-11-13 Cell reprogramming and genetic modification

Country Status (6)

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EP (1) EP1957643A2 (en)
JP (1) JP2009515515A (en)
AU (1) AU2006313518A1 (en)
GB (2) GB2445706A (en)
TW (1) TW200730623A (en)
WO (1) WO2007054720A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4223769A3 (en) 2005-12-13 2023-11-01 Kyoto University Nuclear reprogramming factor
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
GB0701062D0 (en) * 2007-01-19 2007-02-28 Evocell Ltd Biological materials and uses thereof
US20100184051A1 (en) * 2007-05-30 2010-07-22 The General Hospital Corporation Methods of generating pluripotent cells from somatic cells
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
EP2090649A1 (en) 2008-02-13 2009-08-19 Fondazione Telethon Method for reprogramming differentiated cells
AU2015201026B2 (en) * 2008-03-17 2017-03-16 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
SG10201607710UA (en) * 2008-03-17 2016-11-29 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
EP2268809B1 (en) 2008-05-02 2019-02-06 Kyoto University Method of nuclear reprogramming
US10047346B2 (en) 2008-08-08 2018-08-14 Mayo Foundation For Medical Education And Research Method of treating heart tissue using induced pluripotent stem cells
JP5645197B2 (en) * 2009-06-23 2014-12-24 学校法人日本大学 A novel method for maintaining the undifferentiated state of stem cells
US20130029416A1 (en) 2011-07-22 2013-01-31 Tayaramma Thatava Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny
EP3283127B1 (en) 2015-04-15 2020-02-26 University of Massachusetts Compositions and methods for xi chromosome reactivation

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