JP2001513883A - 皮膚組織コレステロールの測定方法 - Google Patents
皮膚組織コレステロールの測定方法Info
- Publication number
- JP2001513883A JP2001513883A JP53652998A JP53652998A JP2001513883A JP 2001513883 A JP2001513883 A JP 2001513883A JP 53652998 A JP53652998 A JP 53652998A JP 53652998 A JP53652998 A JP 53652998A JP 2001513883 A JP2001513883 A JP 2001513883A
- Authority
- JP
- Japan
- Prior art keywords
- cholesterol
- skin
- skin surface
- solution
- hydrogen peroxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Steroid Compounds (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.皮膚表面の一部を準備処置し、所定量の酵素含有混合溶液を前記皮膚表面 に塗布し、露出させ、反応溶液中のコレステロール濃度を評価し、皮膚コレステ ロール濃度を算出するステップを含む皮膚コレステロール測定方法であって、皮 膚が混合溶液と接触する面積を、皮膚に固定されるベースを有する底のないシー ル式容器によって皮膚表面の前記一部に制限し、2.0−2.5ユニットのコレ ステロールオキシダーゼ、0.04〜0.06重量%のデオキシコール酸ナトリ ウム、及び0.1〜0.2重量%の3−(ドデシル−ジメチル−アンモニウム) −プロパンスルホネートをpH=6.8の緩衝液に加えたものを混合溶液として 使用し、過酸化水素のレベルを測定することによって反応溶液中のコレステロー ル濃度を評価することを特徴とする皮膚コレステロール測定方法。 2.3〜5ユニットのコレステロールエステラーゼをも組成物に加えることを 特徴とする請求の範囲第1項に記載の方法。 3.過酸化水素レベルを測定するため、ペルオキシダーゼを基質と共に反応溶 液に加えた後、吸光度測定法を行なうことを特徴とする請求の範囲第1〜2項に 記載の方法。 4.過酸化水素レベルの測定を、電気化学的センサーを反応溶液に浸漬するこ とによって行なうことを特徴とする請求の範囲第1〜2項に記載の方法。 5.過酸化水素レベルの測定を、比色インジケーターを反応溶液に浸漬するこ とによって行なうことを特徴とする請求の範囲第1〜2項に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU97102570A RU2130189C1 (ru) | 1997-02-20 | 1997-02-20 | Способ определения тканевого холестерина кожи |
RU97102570 | 1997-02-20 | ||
PCT/RU1998/000010 WO1998037424A1 (fr) | 1997-02-20 | 1998-01-26 | Methode de mise en evidence de la cholesterine dans les tissus cutanes |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2001513883A true JP2001513883A (ja) | 2001-09-04 |
JP3694324B2 JP3694324B2 (ja) | 2005-09-14 |
Family
ID=20190062
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53652998A Expired - Fee Related JP3694324B2 (ja) | 1997-02-20 | 1998-01-26 | 皮膚組織コレステロールの測定方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US6365363B1 (ja) |
EP (1) | EP0987553A4 (ja) |
JP (1) | JP3694324B2 (ja) |
AU (1) | AU5784698A (ja) |
BR (1) | BR9807594A (ja) |
CA (1) | CA2281769C (ja) |
RU (1) | RU2130189C1 (ja) |
WO (1) | WO1998037424A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003506715A (ja) * | 1999-08-06 | 2003-02-18 | アイエムアイ・インターナショナル・メディカル・イノベーションズ・インコーポレイテッド | 色に基づく生化学的及び免疫学的アッセイにおける分光学的測定 |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6479015B1 (en) * | 1998-03-03 | 2002-11-12 | Pepex Biomedical, Llc | Apparatus for monitoring a level of a chemical species in a body fluid |
US7343185B2 (en) * | 2002-06-21 | 2008-03-11 | Nir Diagnostics Inc. | Measurement of body compounds |
US7811232B1 (en) | 2003-04-21 | 2010-10-12 | Chameleon Development LLC | System for characterizing skin condition |
US7328052B2 (en) * | 2003-09-19 | 2008-02-05 | Nir Diagnostics Inc. | Near infrared risk assessment of diseases |
US20050272112A1 (en) * | 2004-04-28 | 2005-12-08 | Imi International Medical Innovations, Inc. | Direct assay of cholesterol in skin removed by tape stripping |
US7238494B2 (en) * | 2004-04-30 | 2007-07-03 | Imi International Medical Innovations, Inc. | Direct assay of cholesterol in skin samples removed by tape stripping |
US8124367B2 (en) * | 2005-02-28 | 2012-02-28 | Miraculins, Inc. | Device for applying a detector reagent to the skin for measuring cholesterol |
EP1853159A4 (en) * | 2005-03-04 | 2014-12-17 | Covidien Lp | METHOD AND APPARATUS FOR DETERMINING BLOOD ANALYTES |
WO2007028233A1 (en) | 2005-09-06 | 2007-03-15 | Nir Diagnostics Inc. | Method and apparatus for measuring analytes |
US8597208B2 (en) * | 2005-09-06 | 2013-12-03 | Covidien Lp | Method and apparatus for measuring analytes |
WO2007056869A1 (en) * | 2005-11-21 | 2007-05-24 | Nir Diagnostics Inc. | Modified method and apparatus for measuring analytes |
WO2008064130A2 (en) | 2006-11-17 | 2008-05-29 | Bloom Matthew B | Mir spectroscopy of tissue |
TWI544934B (zh) | 2008-06-09 | 2016-08-11 | 愛爾康研究有限公司 | 含有氟喹諾酮抗生素藥物之經改良藥學組成物 |
CN102749462B (zh) * | 2012-07-20 | 2014-08-06 | 常熟柏宇医疗电子有限公司 | 基于生物芯片的无创胆固醇智能监测*** |
CN104181312B (zh) * | 2014-08-08 | 2016-06-01 | 安徽易康达光电科技有限公司 | 一种用于动脉粥样硬化风险评估的皮肤游离胆固醇无创检测装置 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR195000A1 (es) | 1972-05-17 | 1973-08-30 | Boehringer Mannheim Gmbh | Procedimiento para la determinacion de colesterol |
US4226713A (en) | 1978-04-24 | 1980-10-07 | Goldberg Jack M | Diagnostic agents |
US4680259A (en) * | 1984-09-26 | 1987-07-14 | Eastman Kodak Company | Analytical element and method for colorimetric determination of total cholesterol |
US5605160A (en) * | 1986-04-18 | 1997-02-25 | Fishman; Henry | Allergy testing apparatus with variably spaced test testing sites |
SU1675770A1 (ru) * | 1988-01-19 | 1991-09-07 | Научно-исследовательский институт физико-химической медицины | Способ получени средства дл визуальной индикации холестерина на поверхности кожи |
US5489510A (en) | 1988-01-19 | 1996-02-06 | 2860601 Canada Inc. | Method for visual indication of cholesterol on skin surface agents used therefor and methods for producing such agents |
-
1997
- 1997-02-20 RU RU97102570A patent/RU2130189C1/ru active
-
1998
- 1998-01-26 US US09/367,724 patent/US6365363B1/en not_active Expired - Fee Related
- 1998-01-26 AU AU57846/98A patent/AU5784698A/en not_active Abandoned
- 1998-01-26 BR BR9807594-2A patent/BR9807594A/pt not_active Application Discontinuation
- 1998-01-26 CA CA002281769A patent/CA2281769C/en not_active Expired - Fee Related
- 1998-01-26 EP EP98901608A patent/EP0987553A4/en not_active Withdrawn
- 1998-01-26 WO PCT/RU1998/000010 patent/WO1998037424A1/ru active Application Filing
- 1998-01-26 JP JP53652998A patent/JP3694324B2/ja not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003506715A (ja) * | 1999-08-06 | 2003-02-18 | アイエムアイ・インターナショナル・メディカル・イノベーションズ・インコーポレイテッド | 色に基づく生化学的及び免疫学的アッセイにおける分光学的測定 |
Also Published As
Publication number | Publication date |
---|---|
EP0987553A1 (en) | 2000-03-22 |
WO1998037424A1 (fr) | 1998-08-27 |
US6365363B1 (en) | 2002-04-02 |
CA2281769A1 (en) | 1998-08-27 |
CA2281769C (en) | 2006-03-21 |
BR9807594A (pt) | 2000-02-22 |
RU2130189C1 (ru) | 1999-05-10 |
EP0987553A4 (en) | 2004-09-08 |
JP3694324B2 (ja) | 2005-09-14 |
AU5784698A (en) | 1998-09-09 |
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