IL110238A - Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide - Google Patents

Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide

Info

Publication number
IL110238A
IL110238A IL11023894A IL11023894A IL110238A IL 110238 A IL110238 A IL 110238A IL 11023894 A IL11023894 A IL 11023894A IL 11023894 A IL11023894 A IL 11023894A IL 110238 A IL110238 A IL 110238A
Authority
IL
Israel
Prior art keywords
sequence encoding
dna sequence
signal peptide
pct
dna
Prior art date
Application number
IL11023894A
Other languages
English (en)
Other versions
IL110238A0 (en
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of IL110238A0 publication Critical patent/IL110238A0/xx
Publication of IL110238A publication Critical patent/IL110238A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8103—Exopeptidase (E.C. 3.4.11-19) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
IL11023894A 1993-07-08 1994-07-07 Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide IL110238A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK93828A DK82893D0 (da) 1993-07-08 1993-07-08 Peptid

Publications (2)

Publication Number Publication Date
IL110238A0 IL110238A0 (en) 1994-10-21
IL110238A true IL110238A (en) 2003-04-10

Family

ID=8098010

Family Applications (1)

Application Number Title Priority Date Filing Date
IL11023894A IL110238A (en) 1993-07-08 1994-07-07 Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide

Country Status (20)

Country Link
US (1) US5726038A (fr)
EP (1) EP0792367B1 (fr)
JP (1) JP3542604B2 (fr)
KR (1) KR100245173B1 (fr)
AT (1) ATE207124T1 (fr)
AU (1) AU7122194A (fr)
CA (1) CA2166684A1 (fr)
DE (1) DE69428719T2 (fr)
DK (2) DK82893D0 (fr)
ES (1) ES2165881T3 (fr)
HU (1) HU222406B1 (fr)
IL (1) IL110238A (fr)
NZ (1) NZ268346A (fr)
PL (1) PL178924B1 (fr)
PT (1) PT792367E (fr)
RU (1) RU2143495C1 (fr)
SG (1) SG52452A1 (fr)
UA (1) UA40625C2 (fr)
WO (1) WO1995002059A1 (fr)
ZA (1) ZA944912B (fr)

Families Citing this family (42)

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FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
US5861267A (en) * 1995-05-01 1999-01-19 Vertex Pharmaceuticals Incorporated Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
US5772629A (en) * 1995-10-23 1998-06-30 Localmed, Inc. Localized intravascular delivery of TFPI for inhibition of restenosis in recanalized blood vessels
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
EP1365028B1 (fr) 1996-12-13 2009-03-18 Novartis Vaccines and Diagnostics, Inc. Procédés permettant d'exprimer des protéines PDGF ou IGF dans la levure
EP0954589A1 (fr) * 1997-01-24 1999-11-10 Novo Nordisk A/S Nouvelles sequences-guides synthetiques de peptides
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
EP1173590A4 (fr) * 1999-04-30 2003-01-15 Ortho Mcneil Pharm Inc Systeme d'activation du zymogene
AU1995701A (en) 1999-12-29 2001-07-16 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US20030171267A1 (en) * 2000-04-12 2003-09-11 Rosen Craig A. Albumin fusion proteins
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
AU2002310122A1 (en) * 2001-05-25 2002-12-09 Human Genome Sciences, Inc. Chemokine beta-1 fusion proteins
AU2002364587A1 (en) * 2001-12-21 2003-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
EP1997829A1 (fr) * 2001-12-21 2008-12-03 Human Genome Sciences, Inc. Protéines de fusion d'albumine
DE60334109D1 (de) * 2002-06-29 2010-10-21 Leadbio Inc Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
CA2925807C (fr) 2002-09-13 2020-09-15 Cornell Research Foundation, Inc. Utilisation de mutations pour ameliorer les aspergillus phytases
KR100470978B1 (ko) * 2003-01-02 2005-03-10 한국생명공학연구원 앱신 다중 결손 효모 변이 균주 및 이를 이용한 재조합 단백질의 생산 방법
WO2005047508A1 (fr) 2003-11-14 2005-05-26 Novo Nordisk A/S Procede de fabrication d'insuline acylee
EP1692168B1 (fr) 2003-12-03 2011-07-20 Novo Nordisk A/S Insuline monocatenaire
HUE027902T2 (en) * 2004-02-09 2016-11-28 Human Genome Sciences Inc Corp Service Company Albumin fusion proteins
US9056921B2 (en) 2005-08-16 2015-06-16 Novo Nordisk A/S Method for making mature insulin polypeptides
ES2387955T3 (es) 2006-02-27 2012-10-04 Novo Nordisk A/S Derivados de insulina
JP5503968B2 (ja) 2006-09-27 2014-05-28 ノボ・ノルデイスク・エー／エス 成熟インスリンポリペプチドの作製方法
WO2008062010A2 (fr) 2006-11-22 2008-05-29 Novo Nordisk A/S Procédé de fabrication de carboxypeptidases activées
WO2009010428A1 (fr) 2007-07-16 2009-01-22 Novo Nordisk A/S Analogues pegylés de l'insuline, stabilisés vis-à-vis des protéases
KR20110004366A (ko) 2008-03-18 2011-01-13 노보 노르디스크 에이/에스 프로테아제 안정화되고, 아실화된 인슐린 유사체
EP2258855A1 (fr) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Séquences d'expression
EP2649178B8 (fr) 2010-12-09 2017-08-30 Institut Pasteur Méthode basée sur mgmt pour obtenir un haut rendement dans l'expression des protéines recombinantes
DE102011007313A1 (de) * 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa Expressionsverfahren
DE102011118032A1 (de) * 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
EP2788478B1 (fr) 2011-12-09 2017-08-23 Institut Pasteur Immunoessai de dépistage multiplex
US10160988B2 (en) 2012-10-29 2018-12-25 Lonza Ltd Expression sequences
US9707276B2 (en) 2012-12-03 2017-07-18 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues
EP3004156A1 (fr) 2013-06-07 2016-04-13 Novo Nordisk A/S Procédé de fabrication de polypeptides d'insuline mature
PL233560B1 (pl) 2014-12-05 2019-10-31 Mabion Spolka Akcyjna Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka
WO2016144658A1 (fr) 2015-03-10 2016-09-15 Merck Sharp & Dohme Corp. Procédé de préparation d'insuline recombinante par microfiltration
EA037848B1 (ru) 2016-07-14 2021-05-27 Общество С Ограниченной Ответственностью "Биохимический Агент" Гибридный белок, полинуклеотид, генетическая конструкция, продуцент, препарат для регенерации хряща (варианты)
WO2019099615A1 (fr) 2017-11-17 2019-05-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Utilisation de copeptine pour moduler la réponse immunitaire et l'hématopoïèse et stimuler la croissance osseuse
WO2022049409A1 (fr) 2020-09-02 2022-03-10 Sia Terragen Diagnostic express pour sars-cov-2
CN112920280B (zh) * 2021-04-22 2022-07-05 江南大学 一种酸性蛋白酶高效表达的方法及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0243465A1 (fr) * 1985-10-25 1987-11-04 Vivian L. Mackay Procede d'utilisation de bar1 pour la secretion de proteines etrangeres
US5217891A (en) * 1987-07-28 1993-06-08 Chiron Corporation DNA constructs containing a kluyveromyces α factor leader sequence for directing secretion of heterologous polypeptides
US5037743A (en) * 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide

Also Published As

Publication number Publication date
DE69428719T2 (de) 2002-08-08
ATE207124T1 (de) 2001-11-15
HU222406B1 (hu) 2003-06-28
US5726038A (en) 1998-03-10
PT792367E (pt) 2002-04-29
JPH08512202A (ja) 1996-12-24
RU2143495C1 (ru) 1999-12-27
EP0792367A1 (fr) 1997-09-03
PL312436A1 (en) 1996-04-29
SG52452A1 (en) 1998-09-28
HU9600053D0 (en) 1996-03-28
CA2166684A1 (fr) 1995-01-19
JP3542604B2 (ja) 2004-07-14
AU7122194A (en) 1995-02-06
KR100245173B1 (en) 2000-02-15
ZA944912B (en) 1995-03-27
IL110238A0 (en) 1994-10-21
PL178924B1 (pl) 2000-06-30
EP0792367B1 (fr) 2001-10-17
DE69428719D1 (de) 2001-11-22
WO1995002059A1 (fr) 1995-01-19
HUT74253A (en) 1996-11-28
DK82893D0 (da) 1993-07-08
UA40625C2 (uk) 2001-08-15
DK0792367T3 (da) 2001-12-31
ES2165881T3 (es) 2002-04-01
NZ268346A (en) 1997-05-26

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Legal Events

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FF Patent granted
KB Patent renewed
MM9K Patent not in force due to non-payment of renewal fees