IL110238A - Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide - Google Patents
Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptideInfo
- Publication number
- IL110238A IL110238A IL11023894A IL11023894A IL110238A IL 110238 A IL110238 A IL 110238A IL 11023894 A IL11023894 A IL 11023894A IL 11023894 A IL11023894 A IL 11023894A IL 110238 A IL110238 A IL 110238A
- Authority
- IL
- Israel
- Prior art keywords
- sequence encoding
- dna sequence
- signal peptide
- pct
- dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8103—Exopeptidase (E.C. 3.4.11-19) inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK93828A DK82893D0 (da) | 1993-07-08 | 1993-07-08 | Peptid |
Publications (2)
Publication Number | Publication Date |
---|---|
IL110238A0 IL110238A0 (en) | 1994-10-21 |
IL110238A true IL110238A (en) | 2003-04-10 |
Family
ID=8098010
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL11023894A IL110238A (en) | 1993-07-08 | 1994-07-07 | Dna construct comprising a dna sequence encoding the yeast aspartic protease 3 signal peptide |
Country Status (20)
Country | Link |
---|---|
US (1) | US5726038A (fr) |
EP (1) | EP0792367B1 (fr) |
JP (1) | JP3542604B2 (fr) |
KR (1) | KR100245173B1 (fr) |
AT (1) | ATE207124T1 (fr) |
AU (1) | AU7122194A (fr) |
CA (1) | CA2166684A1 (fr) |
DE (1) | DE69428719T2 (fr) |
DK (2) | DK82893D0 (fr) |
ES (1) | ES2165881T3 (fr) |
HU (1) | HU222406B1 (fr) |
IL (1) | IL110238A (fr) |
NZ (1) | NZ268346A (fr) |
PL (1) | PL178924B1 (fr) |
PT (1) | PT792367E (fr) |
RU (1) | RU2143495C1 (fr) |
SG (1) | SG52452A1 (fr) |
UA (1) | UA40625C2 (fr) |
WO (1) | WO1995002059A1 (fr) |
ZA (1) | ZA944912B (fr) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2686899B1 (fr) * | 1992-01-31 | 1995-09-01 | Rhone Poulenc Rorer Sa | Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant. |
US6500645B1 (en) | 1994-06-17 | 2002-12-31 | Novo Nordisk A/S | N-terminally extended proteins expressed in yeast |
US5861267A (en) * | 1995-05-01 | 1999-01-19 | Vertex Pharmaceuticals Incorporated | Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity |
US5772629A (en) * | 1995-10-23 | 1998-06-30 | Localmed, Inc. | Localized intravascular delivery of TFPI for inhibition of restenosis in recanalized blood vessels |
ZA9610456B (en) * | 1995-12-20 | 1997-06-20 | Novo Nordisk As | N-terminally extended proteins expressed in yeast |
EP1365028B1 (fr) | 1996-12-13 | 2009-03-18 | Novartis Vaccines and Diagnostics, Inc. | Procédés permettant d'exprimer des protéines PDGF ou IGF dans la levure |
EP0954589A1 (fr) * | 1997-01-24 | 1999-11-10 | Novo Nordisk A/S | Nouvelles sequences-guides synthetiques de peptides |
US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
US6420157B1 (en) * | 1999-04-30 | 2002-07-16 | Ortho-Mcneil Pharmaceutical, Inc. | Zymogen activation system |
EP1173590A4 (fr) * | 1999-04-30 | 2003-01-15 | Ortho Mcneil Pharm Inc | Systeme d'activation du zymogene |
AU1995701A (en) | 1999-12-29 | 2001-07-16 | Novo Nordisk A/S | Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast |
US20030171267A1 (en) * | 2000-04-12 | 2003-09-11 | Rosen Craig A. | Albumin fusion proteins |
US20050100991A1 (en) * | 2001-04-12 | 2005-05-12 | Human Genome Sciences, Inc. | Albumin fusion proteins |
AU2002310122A1 (en) * | 2001-05-25 | 2002-12-09 | Human Genome Sciences, Inc. | Chemokine beta-1 fusion proteins |
AU2002364587A1 (en) * | 2001-12-21 | 2003-07-30 | Human Genome Sciences, Inc. | Albumin fusion proteins |
EP1997829A1 (fr) * | 2001-12-21 | 2008-12-03 | Human Genome Sciences, Inc. | Protéines de fusion d'albumine |
DE60334109D1 (de) * | 2002-06-29 | 2010-10-21 | Leadbio Inc | Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit |
CA2925807C (fr) | 2002-09-13 | 2020-09-15 | Cornell Research Foundation, Inc. | Utilisation de mutations pour ameliorer les aspergillus phytases |
KR100470978B1 (ko) * | 2003-01-02 | 2005-03-10 | 한국생명공학연구원 | 앱신 다중 결손 효모 변이 균주 및 이를 이용한 재조합 단백질의 생산 방법 |
WO2005047508A1 (fr) | 2003-11-14 | 2005-05-26 | Novo Nordisk A/S | Procede de fabrication d'insuline acylee |
EP1692168B1 (fr) | 2003-12-03 | 2011-07-20 | Novo Nordisk A/S | Insuline monocatenaire |
HUE027902T2 (en) * | 2004-02-09 | 2016-11-28 | Human Genome Sciences Inc Corp Service Company | Albumin fusion proteins |
US9056921B2 (en) | 2005-08-16 | 2015-06-16 | Novo Nordisk A/S | Method for making mature insulin polypeptides |
ES2387955T3 (es) | 2006-02-27 | 2012-10-04 | Novo Nordisk A/S | Derivados de insulina |
JP5503968B2 (ja) | 2006-09-27 | 2014-05-28 | ノボ・ノルデイスク・エー/エス | 成熟インスリンポリペプチドの作製方法 |
WO2008062010A2 (fr) | 2006-11-22 | 2008-05-29 | Novo Nordisk A/S | Procédé de fabrication de carboxypeptidases activées |
WO2009010428A1 (fr) | 2007-07-16 | 2009-01-22 | Novo Nordisk A/S | Analogues pegylés de l'insuline, stabilisés vis-à-vis des protéases |
KR20110004366A (ko) | 2008-03-18 | 2011-01-13 | 노보 노르디스크 에이/에스 | 프로테아제 안정화되고, 아실화된 인슐린 유사체 |
EP2258855A1 (fr) | 2009-05-28 | 2010-12-08 | Universität für Bodenkultur Wien | Séquences d'expression |
EP2649178B8 (fr) | 2010-12-09 | 2017-08-30 | Institut Pasteur | Méthode basée sur mgmt pour obtenir un haut rendement dans l'expression des protéines recombinantes |
DE102011007313A1 (de) * | 2011-04-13 | 2012-10-18 | Henkel Ag & Co. Kgaa | Expressionsverfahren |
DE102011118032A1 (de) * | 2011-05-31 | 2012-12-06 | Henkel Ag & Co. Kgaa | Expressionsvektoren zur verbesserten Proteinsekretion |
EP2788478B1 (fr) | 2011-12-09 | 2017-08-23 | Institut Pasteur | Immunoessai de dépistage multiplex |
US10160988B2 (en) | 2012-10-29 | 2018-12-25 | Lonza Ltd | Expression sequences |
US9707276B2 (en) | 2012-12-03 | 2017-07-18 | Merck Sharp & Dohme Corp. | O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues |
EP3004156A1 (fr) | 2013-06-07 | 2016-04-13 | Novo Nordisk A/S | Procédé de fabrication de polypeptides d'insuline mature |
PL233560B1 (pl) | 2014-12-05 | 2019-10-31 | Mabion Spolka Akcyjna | Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka |
WO2016144658A1 (fr) | 2015-03-10 | 2016-09-15 | Merck Sharp & Dohme Corp. | Procédé de préparation d'insuline recombinante par microfiltration |
EA037848B1 (ru) | 2016-07-14 | 2021-05-27 | Общество С Ограниченной Ответственностью "Биохимический Агент" | Гибридный белок, полинуклеотид, генетическая конструкция, продуцент, препарат для регенерации хряща (варианты) |
WO2019099615A1 (fr) | 2017-11-17 | 2019-05-23 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Utilisation de copeptine pour moduler la réponse immunitaire et l'hématopoïèse et stimuler la croissance osseuse |
WO2022049409A1 (fr) | 2020-09-02 | 2022-03-10 | Sia Terragen | Diagnostic express pour sars-cov-2 |
CN112920280B (zh) * | 2021-04-22 | 2022-07-05 | 江南大学 | 一种酸性蛋白酶高效表达的方法及其应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0243465A1 (fr) * | 1985-10-25 | 1987-11-04 | Vivian L. Mackay | Procede d'utilisation de bar1 pour la secretion de proteines etrangeres |
US5217891A (en) * | 1987-07-28 | 1993-06-08 | Chiron Corporation | DNA constructs containing a kluyveromyces α factor leader sequence for directing secretion of heterologous polypeptides |
US5037743A (en) * | 1988-08-05 | 1991-08-06 | Zymogenetics, Inc. | BAR1 secretion signal |
US5538863A (en) * | 1993-07-01 | 1996-07-23 | Immunex Corporation | Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide |
-
1993
- 1993-07-08 DK DK93828A patent/DK82893D0/da not_active Application Discontinuation
-
1994
- 1994-07-07 IL IL11023894A patent/IL110238A/xx not_active IP Right Cessation
- 1994-07-07 ZA ZA944912A patent/ZA944912B/xx unknown
- 1994-07-08 DE DE69428719T patent/DE69428719T2/de not_active Expired - Lifetime
- 1994-07-08 SG SG1996004717A patent/SG52452A1/en unknown
- 1994-07-08 CA CA002166684A patent/CA2166684A1/fr not_active Abandoned
- 1994-07-08 HU HU9600053A patent/HU222406B1/hu not_active IP Right Cessation
- 1994-07-08 JP JP50376895A patent/JP3542604B2/ja not_active Expired - Lifetime
- 1994-07-08 ES ES94920419T patent/ES2165881T3/es not_active Expired - Lifetime
- 1994-07-08 US US08/446,646 patent/US5726038A/en not_active Expired - Lifetime
- 1994-07-08 WO PCT/DK1994/000281 patent/WO1995002059A1/fr active IP Right Grant
- 1994-07-08 PT PT94920419T patent/PT792367E/pt unknown
- 1994-07-08 PL PL94312436A patent/PL178924B1/pl not_active IP Right Cessation
- 1994-07-08 AU AU71221/94A patent/AU7122194A/en not_active Abandoned
- 1994-07-08 NZ NZ268346A patent/NZ268346A/en unknown
- 1994-07-08 EP EP94920419A patent/EP0792367B1/fr not_active Expired - Lifetime
- 1994-07-08 UA UA96010072A patent/UA40625C2/uk unknown
- 1994-07-08 AT AT94920419T patent/ATE207124T1/de not_active IP Right Cessation
- 1994-07-08 RU RU96103364/13A patent/RU2143495C1/ru not_active IP Right Cessation
- 1994-07-08 DK DK94920419T patent/DK0792367T3/da active
-
1996
- 1996-01-08 KR KR1019960700049A patent/KR100245173B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE69428719T2 (de) | 2002-08-08 |
ATE207124T1 (de) | 2001-11-15 |
HU222406B1 (hu) | 2003-06-28 |
US5726038A (en) | 1998-03-10 |
PT792367E (pt) | 2002-04-29 |
JPH08512202A (ja) | 1996-12-24 |
RU2143495C1 (ru) | 1999-12-27 |
EP0792367A1 (fr) | 1997-09-03 |
PL312436A1 (en) | 1996-04-29 |
SG52452A1 (en) | 1998-09-28 |
HU9600053D0 (en) | 1996-03-28 |
CA2166684A1 (fr) | 1995-01-19 |
JP3542604B2 (ja) | 2004-07-14 |
AU7122194A (en) | 1995-02-06 |
KR100245173B1 (en) | 2000-02-15 |
ZA944912B (en) | 1995-03-27 |
IL110238A0 (en) | 1994-10-21 |
PL178924B1 (pl) | 2000-06-30 |
EP0792367B1 (fr) | 2001-10-17 |
DE69428719D1 (de) | 2001-11-22 |
WO1995002059A1 (fr) | 1995-01-19 |
HUT74253A (en) | 1996-11-28 |
DK82893D0 (da) | 1993-07-08 |
UA40625C2 (uk) | 2001-08-15 |
DK0792367T3 (da) | 2001-12-31 |
ES2165881T3 (es) | 2002-04-01 |
NZ268346A (en) | 1997-05-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR100245173B1 (en) | A dna construct encoding the yap3 signal peptide | |
GB2301365B (en) | Albumin secreted from yeast having reduced levels of aspartyl protease 3 proteolytic activity, and Yap3p-resistant albumin | |
ATE256191T1 (de) | Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae | |
MXPA03000786A (es) | Peptidos de promiostatina y metodos de usar los mismos. | |
BR8506902A (pt) | Segmento de toxina de gene de proteina cristal bacillus thuringiensis | |
AU2412195A (en) | Recombinant proteins of filamentous haemagglutinin of (bordetella), particularly (bordetella pertussis), method for producing same, and uses thereof for producing foreign proteins or vaccinating active principles | |
AU5628394A (en) | Granulocyte chemotactic protein | |
AU4333793A (en) | Modified kluyveromyces yeasts, their preparation and use | |
ATE171217T1 (de) | Methoden zur synthese und reinigung von peptiden | |
DK91092D0 (fr) | ||
EP1272514A4 (fr) | Adn codant pour le recepteur humain de la vanilloide vr3 | |
DE69133340D1 (de) | Neue type-i-interferon varianten, ihre verfahren zur herstellung, und ihre verwendungen | |
HU9601298D0 (en) | Endothelin-converting enzyme | |
EP0945512A3 (fr) | Sérine protéases | |
KR910000180A (ko) | 트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법 | |
JPS6486876A (en) | Variant human lysozyme |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FF | Patent granted | ||
KB | Patent renewed | ||
MM9K | Patent not in force due to non-payment of renewal fees |