KR910000180A - 트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법 - Google Patents
트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법 Download PDFInfo
- Publication number
- KR910000180A KR910000180A KR1019900008461A KR900008461A KR910000180A KR 910000180 A KR910000180 A KR 910000180A KR 1019900008461 A KR1019900008461 A KR 1019900008461A KR 900008461 A KR900008461 A KR 900008461A KR 910000180 A KR910000180 A KR 910000180A
- Authority
- KR
- South Korea
- Prior art keywords
- inhibitory activity
- polypeptide
- plasmid
- thrombin inhibitory
- secretory
- Prior art date
Links
- 108090000190 Thrombin Proteins 0.000 title claims 7
- 230000002401 inhibitory effect Effects 0.000 title claims 7
- 229920001184 polypeptide Polymers 0.000 title claims 7
- 108090000765 processed proteins & peptides Proteins 0.000 title claims 7
- 102000004196 processed proteins & peptides Human genes 0.000 title claims 7
- 229960004072 thrombin Drugs 0.000 title claims 7
- 238000002360 preparation method Methods 0.000 title 1
- 239000013612 plasmid Substances 0.000 claims description 11
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
- 230000003248 secreting effect Effects 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 230000028327 secretion Effects 0.000 claims description 2
- 239000002243 precursor Substances 0.000 claims 4
- 244000063299 Bacillus subtilis Species 0.000 claims 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 108090000145 Bacillolysin Proteins 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims 1
- 108091005507 Neutral proteases Proteins 0.000 claims 1
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 239000012228 culture supernatant Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 210000003705 ribosome Anatomy 0.000 claims 1
- 102000007625 Hirudins Human genes 0.000 description 3
- 108010007267 Hirudins Proteins 0.000 description 3
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 description 3
- 229940006607 hirudin Drugs 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F02—COMBUSTION ENGINES; HOT-GAS OR COMBUSTION-PRODUCT ENGINE PLANTS
- F02B—INTERNAL-COMBUSTION PISTON ENGINES; COMBUSTION ENGINES IN GENERAL
- F02B1/00—Engines characterised by fuel-air mixture compression
- F02B1/02—Engines characterised by fuel-air mixture compression with positive ignition
- F02B1/04—Engines characterised by fuel-air mixture compression with positive ignition with fuel-air mixture admission into cylinder
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Hematology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Diabetes (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 이종 단백질 발현 분비 백터, pNPA225를 제조하는 방법을 나타낸다.
제2도는 HV1형 히루딘 분비플라스마, pHph208를 제조하는 방법을 나타낸다.
제3도는 히루딘을 코딩하는 DNA서열을 함유하는 하이브리드 플라스미드 p3009를 제조하는 방법을 나타낸다.
제4도는 히루딘을 분비하는 플라스미드, pNPH141를 제조하는 방법을 나타낸다.
Claims (14)
- 하기 아미노산 서열을 갖는, 트롬빈 억제 활성을 갖는 폴리펩티드의 전구체,
- 하기 아미노산 서열을 갖는, 트롬빈 억제 활성을 갖는 폴리펩티드,
- 제1항에 기재된 트롬빈 억제 활성을 갖는 폴리펩티드의 전구체를 코딩하는 하기의 DNA 서열.
- 제2항에 기재된 트롬빈 억제 활성을 갖는 폴리펩티드의 전구체를 코딩하는 하기의 DNA 서열.
- (a)프로모터 영역 및 리보소옴 결합 부위를 코팅하는 영역을 함유하는 DNA서열, (b) DNA서열(a)의 3'말단에 결합 가능한, 제1항에 기재된 전구체를 코딩하는 DNA서열, 및 (c)숙주에서 복제가능한 벡터DNA를 함유하는 분비 플라스미드.
- 제5항에 있어서, DNA서열(a)내의 여역이 바실루스 아밀로리퀴파시엔스(Bacillus amyloliquifaciens)의 중성 프로테아제 유전자의 것이 분비 플라스미드.
- 제6항에 있어서, (a)와 (b)의 결합 DNA 서열이 하기와 같은 분비 플라스미드.
- 제5항 내지 제7항중 어느 한 항에 있어서, 벡터 DNA가 바실루스속의 세균에서 복제 가능한 플라스미드인 분비 플라스미드.
- 제8항에 있어서, 프라스미드가 pUB110인 분비 플라스미드.
- 제9항에 있어서, 분비 플라스미드가 pNPH141인 분비 플라스미드.
- 제5항 내지 제10항에 기재된 분비 플라스미드 중의 하나를 사용하여 형질 전환된 형질전환체.
- 제11항에 있어서, 형질전환된 미생물이 바실루스 서브틸리스 (Bacillus subtilis)인 형질전환체.
- 제12항에 있어서, 바실루스 서브틸리스가 바실루스 서브틸리스 FERM BP-2403인 형질전환체.
- 제12항 또는 제13항의 형질 전환체를 배양하고 배양 상충액으로부터 트롬빈 억제 활성을 갖는 폴리펩티드를 회수하는 단계를 특징으로 하는, 트롬빈 억제 활성을 갖는 폴리펩티드의 제조방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1145489A JP2859297B2 (ja) | 1989-06-09 | 1989-06-09 | トロンビン阻害活性を有するポリペプチドおよびその製造法 |
JP?1-145489 | 1989-06-09 | ||
JP1-145489 | 1989-06-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR910000180A true KR910000180A (ko) | 1991-01-29 |
KR930003913B1 KR930003913B1 (ko) | 1993-05-15 |
Family
ID=15386446
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019900008461A KR930003913B1 (ko) | 1989-06-09 | 1990-06-09 | 트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법 |
Country Status (6)
Country | Link |
---|---|
US (1) | US5166318A (ko) |
EP (1) | EP0402159B1 (ko) |
JP (1) | JP2859297B2 (ko) |
KR (1) | KR930003913B1 (ko) |
CA (1) | CA2018211C (ko) |
DE (1) | DE69013403T2 (ko) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5624822A (en) * | 1989-12-22 | 1997-04-29 | Basf Aktiengesellschaft | Hirudin fusion proteins and preparation of hirudin |
EP0624642B1 (en) * | 1993-05-12 | 1999-01-20 | Indian Council For Medical Research | Novel thrombolytic agent, process for preparing same and its use for the preparation of a thrombi dissolving medicament |
CN115572329B (zh) * | 2021-06-21 | 2024-02-06 | 王大勇 | 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0151760A1 (en) * | 1984-01-06 | 1985-08-21 | Takeda Chemical Industries, Ltd. | Novel DNA and use thereof |
GB2171703B (en) * | 1985-01-18 | 1989-11-15 | Agency Ind Science Techn | Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re |
FR2611723B1 (fr) * | 1987-02-27 | 1989-09-08 | Transgene Sa | Vecteur d'expression d'adn recombinant codant pour l'hirudine, cultures de cellules de mammiferes infectees ou transformees, procede de preparation de l'hirudine et proteine obtenue |
-
1989
- 1989-06-09 JP JP1145489A patent/JP2859297B2/ja not_active Expired - Lifetime
-
1990
- 1990-06-04 CA CA002018211A patent/CA2018211C/en not_active Expired - Fee Related
- 1990-06-04 US US07/531,403 patent/US5166318A/en not_active Expired - Lifetime
- 1990-06-08 EP EP90306248A patent/EP0402159B1/en not_active Expired - Lifetime
- 1990-06-08 DE DE69013403T patent/DE69013403T2/de not_active Expired - Fee Related
- 1990-06-09 KR KR1019900008461A patent/KR930003913B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE69013403T2 (de) | 1995-02-23 |
US5166318A (en) | 1992-11-24 |
JPH0310694A (ja) | 1991-01-18 |
DE69013403D1 (de) | 1994-11-24 |
KR930003913B1 (ko) | 1993-05-15 |
JP2859297B2 (ja) | 1999-02-17 |
EP0402159A1 (en) | 1990-12-12 |
EP0402159B1 (en) | 1994-10-19 |
CA2018211C (en) | 1996-02-20 |
AU620721B2 (en) | 1992-02-20 |
AU5697090A (en) | 1990-12-13 |
CA2018211A1 (en) | 1990-12-09 |
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