HUP0203693A2 - Növényi gén expressziója konstitutív növényi V-ATP-áz promoterek irányítása alatt - Google Patents

Növényi gén expressziója konstitutív növényi V-ATP-áz promoterek irányítása alatt

Info

Publication number
HUP0203693A2
HUP0203693A2 HU0203693A HUP0203693A HUP0203693A2 HU P0203693 A2 HUP0203693 A2 HU P0203693A2 HU 0203693 A HU0203693 A HU 0203693A HU P0203693 A HUP0203693 A HU P0203693A HU P0203693 A2 HUP0203693 A2 HU P0203693A2
Authority
HU
Hungary
Prior art keywords
plant
controlled
promoters
gene expression
plants
Prior art date
Application number
HU0203693A
Other languages
English (en)
Inventor
Elke Duwenig
Thomas Rausch
Original Assignee
Basf Plant Science Gmbh.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh. filed Critical Basf Plant Science Gmbh.
Publication of HUP0203693A2 publication Critical patent/HUP0203693A2/hu

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • C12N15/8238Externally regulated expression systems chemically inducible, e.g. tetracycline
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • C12N15/8239Externally regulated expression systems pathogen inducible
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)

Abstract

A találmány tárgyát olyan DNS-szerkezetek képezik, amelyek egy növényiV-ATPáz-promotert tartalmaznak működőképes módon összekapcsolva egyheterológ génnel. A találmány tárgyához tartozik az ilyen DNS-szerkezetek létrehozása expressziós keretek és rekombináns vektorokformájában, továbbá az előbbiekkel transzformált növények, növényisejtek vagy protoplasztok is. A találmány tárgyát képezi különösen aBeta vulgaris V-ATPáz promotere c alegységének 2. izoformja. Aszelekciós és rezisztencia gének kifejeztetéséhez előnyös, ha anövényekben olyan konstitutív promoterek állnak rendelkezésre, amelyekerős és folyamatos aktivitást fejtenek ki lehetőleg a növény összesszövetében és sejttípusában. Különösen előnyös, ha ezek a promoterekstressz hatására aktívabbá válnak, vagy legalább nem veszítenekkorábbi aktivitásukból. Ó
HU0203693A 1999-08-26 2000-08-10 Növényi gén expressziója konstitutív növényi V-ATP-áz promoterek irányítása alatt HUP0203693A2 (hu)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15088799P 1999-08-26 1999-08-26
DE19940648 1999-08-26
PCT/EP2000/007774 WO2001014572A2 (de) 1999-08-26 2000-08-10 Pflanzliche genexpression unter der kontrolle von konstitutiven pflanzlichen v-atpase promotoren

Publications (1)

Publication Number Publication Date
HUP0203693A2 true HUP0203693A2 (hu) 2003-04-28

Family

ID=26054732

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0203693A HUP0203693A2 (hu) 1999-08-26 2000-08-10 Növényi gén expressziója konstitutív növényi V-ATP-áz promoterek irányítása alatt

Country Status (15)

Country Link
EP (1) EP1206560B1 (hu)
JP (1) JP2003507074A (hu)
CN (1) CN1371425A (hu)
AR (1) AR033649A1 (hu)
AT (1) ATE309381T1 (hu)
AU (1) AU780117B2 (hu)
BR (1) BR0013537A (hu)
CA (1) CA2379498C (hu)
DE (1) DE50011579D1 (hu)
HU (1) HUP0203693A2 (hu)
IL (1) IL147950A0 (hu)
MX (1) MXPA02001786A (hu)
PL (1) PL354490A1 (hu)
RU (1) RU2002107795A (hu)
WO (1) WO2001014572A2 (hu)

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Also Published As

Publication number Publication date
EP1206560A2 (de) 2002-05-22
JP2003507074A (ja) 2003-02-25
WO2001014572A3 (de) 2001-10-11
CN1371425A (zh) 2002-09-25
IL147950A0 (en) 2002-08-14
DE50011579D1 (de) 2005-12-15
WO2001014572A2 (de) 2001-03-01
BR0013537A (pt) 2002-04-30
MXPA02001786A (es) 2003-07-14
CA2379498C (en) 2012-12-18
AU7509100A (en) 2001-03-19
ATE309381T1 (de) 2005-11-15
AR033649A1 (es) 2004-01-07
CA2379498A1 (en) 2001-03-01
PL354490A1 (en) 2004-01-26
EP1206560B1 (de) 2005-11-09
AU780117B2 (en) 2005-03-03
RU2002107795A (ru) 2004-03-10

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