BR0013537A - Construção de dna com um promotor da v-atpase vegetal ou seu equivalente funcional, polincucleotìdeo, vetor recombinante, microorganismo, célula ou protoplastro de vegetal transgênico, métodos para a expressão controlada de um gene heterólogico em uma célula vegetal ou um protoplasto e para produzir uma proteìna recombinante, usos da construção de dna, e de um promotor da v-atpase vegetal, proteìna recombinante, célula vegetal ou protoplasto, e, vegetal - Google Patents

Construção de dna com um promotor da v-atpase vegetal ou seu equivalente funcional, polincucleotìdeo, vetor recombinante, microorganismo, célula ou protoplastro de vegetal transgênico, métodos para a expressão controlada de um gene heterólogico em uma célula vegetal ou um protoplasto e para produzir uma proteìna recombinante, usos da construção de dna, e de um promotor da v-atpase vegetal, proteìna recombinante, célula vegetal ou protoplasto, e, vegetal

Info

Publication number
BR0013537A
BR0013537A BR0013537-2A BR0013537A BR0013537A BR 0013537 A BR0013537 A BR 0013537A BR 0013537 A BR0013537 A BR 0013537A BR 0013537 A BR0013537 A BR 0013537A
Authority
BR
Brazil
Prior art keywords
plant
atpase
promoter
dna
plant cell
Prior art date
Application number
BR0013537-2A
Other languages
English (en)
Inventor
Elke Duwenig
Thomas Rausch
Original Assignee
Basf Plant Science Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh filed Critical Basf Plant Science Gmbh
Publication of BR0013537A publication Critical patent/BR0013537A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Communicable Diseases (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"CONSTRUçãO DE DNA COM UM PROMOTOR DA V-ATPASE VEGETAL OU SEU EQUIVALENTE FUNCIONAL, POLINUCLEOTìDEO, VETOR RECOMBINANTE, MICROORGANISMO, CéLULA OU PROTOPLASTO DE VEGETAL TRANSGêNICO, VEGETAL TRANSGêNICO, MéTODOS PARA A EXPRESSãO CONTROLADA DE UM GENE HETERóLOGO EM UMA CéLULA VEGETAL OU UM PROTOPLASTO E PARA PRODUZIR UMA PROTEìNA RECOMBINANTE, USOS DA CONSTRUçãO DE DNA, E DE UM PROMOTOR DA V-ATPASE VEGETAL, PROTEìNA RECOMBINANTE, CéLULA VEGETAL OU PROTOPLASTO, E, VEGETAL". Para expressar os genes de seleção e de resistência, é desejável ter promotores constitutivos vegetais que tenham atividade constitutiva forte e homogênea, se possível, em todos os tecidos vegetais ou tipos de células e que, além disso, tornam-se mais ativos ou não podem ser reprimidos em condições de estresse. A invenção diz respeito à síntese de DNA que contém um promotor da V-ATPase vegetal que está operacionalmente ligado a um gene heterólogo. A invenção também diz respeito ao uso desta síntese na forma de cassetes de expressão, vetores recombinantes e em plantas, células vegetais ou protoplastos transgênicos. A invenção diz respeito, em particular, ao promotor da isoforma 2 da subunidade c da V-ATPase do Beta vulgaris.
BR0013537-2A 1999-08-26 2000-08-10 Construção de dna com um promotor da v-atpase vegetal ou seu equivalente funcional, polincucleotìdeo, vetor recombinante, microorganismo, célula ou protoplastro de vegetal transgênico, métodos para a expressão controlada de um gene heterólogico em uma célula vegetal ou um protoplasto e para produzir uma proteìna recombinante, usos da construção de dna, e de um promotor da v-atpase vegetal, proteìna recombinante, célula vegetal ou protoplasto, e, vegetal BR0013537A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15088799P 1999-08-26 1999-08-26
DE19940648 1999-08-26
PCT/EP2000/007774 WO2001014572A2 (de) 1999-08-26 2000-08-10 Pflanzliche genexpression unter der kontrolle von konstitutiven pflanzlichen v-atpase promotoren

Publications (1)

Publication Number Publication Date
BR0013537A true BR0013537A (pt) 2002-04-30

Family

ID=26054732

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0013537-2A BR0013537A (pt) 1999-08-26 2000-08-10 Construção de dna com um promotor da v-atpase vegetal ou seu equivalente funcional, polincucleotìdeo, vetor recombinante, microorganismo, célula ou protoplastro de vegetal transgênico, métodos para a expressão controlada de um gene heterólogico em uma célula vegetal ou um protoplasto e para produzir uma proteìna recombinante, usos da construção de dna, e de um promotor da v-atpase vegetal, proteìna recombinante, célula vegetal ou protoplasto, e, vegetal

Country Status (15)

Country Link
EP (1) EP1206560B1 (pt)
JP (1) JP2003507074A (pt)
CN (1) CN1371425A (pt)
AR (1) AR033649A1 (pt)
AT (1) ATE309381T1 (pt)
AU (1) AU780117B2 (pt)
BR (1) BR0013537A (pt)
CA (1) CA2379498C (pt)
DE (1) DE50011579D1 (pt)
HU (1) HUP0203693A2 (pt)
IL (1) IL147950A0 (pt)
MX (1) MXPA02001786A (pt)
PL (1) PL354490A1 (pt)
RU (1) RU2002107795A (pt)
WO (1) WO2001014572A2 (pt)

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Also Published As

Publication number Publication date
EP1206560B1 (de) 2005-11-09
EP1206560A2 (de) 2002-05-22
IL147950A0 (en) 2002-08-14
CA2379498A1 (en) 2001-03-01
HUP0203693A2 (hu) 2003-04-28
JP2003507074A (ja) 2003-02-25
AR033649A1 (es) 2004-01-07
DE50011579D1 (de) 2005-12-15
MXPA02001786A (es) 2003-07-14
WO2001014572A2 (de) 2001-03-01
CA2379498C (en) 2012-12-18
ATE309381T1 (de) 2005-11-15
PL354490A1 (en) 2004-01-26
AU7509100A (en) 2001-03-19
RU2002107795A (ru) 2004-03-10
AU780117B2 (en) 2005-03-03
WO2001014572A3 (de) 2001-10-11
CN1371425A (zh) 2002-09-25

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