HUE033091T2 - Tejtermék és eljárás ennek elõállítására - Google Patents

Description

(12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: A23C 191032 <200e 01> A23C 91127 <2006 01> 16.11.2016 Bulletin 2016/46 C12N15100<200601> A23C 9I12<200601> A23C 191068 <200601> (21) Application number: 06700928.2 (86) International application number: (22) Date of filing: 11.01.2006 PCT/JP2006/300574 (87) International publication number: WO 2006/075772 (20.07.2006 Gazette 2006/29)

(54) DAIRY PRODUCT AND PROCESS FOR PRODUCTION THEREOF

MILCHPRODUKT UND VERFAHREN ZU SEINER HERSTELLUNG PRODUIT LAITIER ET PROCEDE POUR LE PRODUIRE (84) Designated Contracting States: · NAKAGOSHI, Hiroyuki AT BE BG CH CY CZ DE DK EE ES FI FR GB GR -ku, Kawasaki-shi, Kanagawa, 2108681 (JP) HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI · MIWA, Noriko SK TR -ku, Kawasaki-shi, Kanagawa, 2108681 (JP) • NAKAMURA, Nami (30) Priority: 13.01.2005 JP 2005005854 -ku, Kawasaki-shi, Kanagawa, 2108681 (JP) • WAKABAYASHI, Hidehiko (43) Date of publication of application: -ku, Kawasaki-shi, Kanagawa, 2108681 (JP) 03.10.2007 Bulletin 2007/40 (74) Representative: Strehl Schiibel-Hopf &amp; Partner (73) Proprietors: Maximilianstrasse 54 • Ajinomoto Co., Inc. 80538 Miinchen (DE)

Tokyo 104-0031 (JP) • Amano Enzyme Inc. (56) References cited:

Nagoya-shi, DE-A1-2 044 866 JP-A- 11 042 086

Aichi 460-0003 (JP) JP-A- 2000 014 317 JP-A- 2001 120 179 US-A- 3 796 633 (72) Inventors: • KODERA, Tomohiro -ku, Kawasaki-shi, Kanagawa, 2108681 (JP)

Description

Technical Field [0001] The present invention relates to dairy products which have been manufactured by using a protein deamidating enzyme and present smooth oral sensation with suppressed acidic taste and bitter taste, and a method of manufacturing the same.

Background Art [0002] Dairy products such as cheese, yogurt and the like have been once unfamiliarfoodstuffs for Japanese. However, in recent years, their consumption is increasing due to their health and nutrition related functions, and a variety of dairy products are on the market to meet diversified food preference of consumers.

[0003] As for utilization of enzymes for manufacturing dairy products, rennet that is a milk-clotting enzyme for cheese is well-known, while a method utilizing a transglutaminase for cheese (Japanese Patent Application Laid-Open (Kokai) No. Hei 8-173032) and a method utilizing the same for yogurt (Japanese Patent Application Laid-Open (Kokai) No. Hei 6-197688) are also known. As for utilization of a protein deamidating enzyme, Japanese Patent Application Laid-Open (Kokai) No. 2000-50887 discloses a method for using a protein deamidating enzyme to deamidate casein, thereby to improve the dispersibility and solubility thereof, and a method of manufacturing pudding-like foods which comprises allowing a transglutaminase to act on concentrated milk, wherein a protein deamidating enzyme is added to terminate the transglutaminase action. Japanese Patent Application Laid-Open (Kokai) No. 2001-218590 discloses a method wherein a protein deamidating enzyme is allowed to act on a milk caseinate or a whey protein, in order to deamidate, thereby to improve the foaming properties, emulsification, and solubility thereof. Japanese Patent Application Laid-Open (Kokai) No. 2003-250460 discloses a method wherein a protein deamidating enzyme is allowed to acton β-lactoglobulin, in order to deamidate, thereby to improve the properties in foaming and emulsification. However, these patent documents do not describe a method for providing a dairy product according to the present invention which presents smooth oral sensation with suppressed acidic taste and bitter taste, particularly cheese and yogurt which present smooth oral sensation with suppressed acidic taste and bitter taste.

[0004] US 3 796 633 A and DE 20 44 866 A1 disclose a method for preparing Gouda type cheese wherein milk is treated with rennet and a peptido-glutaminase followed by further processing to give cheese having enhanced flavour.

[0005] JP 2001 120179 A discloses a method for preparing fermented milk wherein glutaminase is used as additive and various lactic acid bacteria are used for fermentation.

Disclosure of the Invention [0006] The present invention is to provide a dairy product which presents smooth oral sensation with suppressed acidic taste, and a method for manufacturing the same, in order to meet diversified preference in taste of consumers.

[0007] The present inventors have made an intensive and extensive study to solve the above-mentioned problems, and found that a protein deamidating enzyme can be added to raw milk to act on the milk protein in the raw milk, thereby to resolve the above-mentioned problem, and completed the present invention. Namely, the present invention comprises the followings: [1] A method for manufacturing a dairy product, wherein a protein deamidating enzyme is added to raw milk and allowed to act on the milk protein in the raw milk, wherein the protein deamidating enzyme acts directly on the amide groups of a protein and has an action to deamidate with neither peptide bond cleavage nor protein crosslink accompanied.

[2] The method according to [1] above, wherein said dairy product is cheese or yogurt.

[3] The method according to [1] or [2] above, wherein said protein deamidating enzyme is added in an amount of 0.1 to 500 units per 1 L of said raw milk.

[4] A dairy product obtained by any one of [1] to [3] above.

[0008] The protein deamidating enzyme used in the method according to the present invention acts directly on the amide groups of a protein and has an action to deamidate with neither peptide bond cleavage nor protein crosslink accompanied. The protein deamidating. enzyme is not particularly limited in type so far as it has the action. An enzyme disclosed in Japanese Patent Application Laid-Open (Kokai) No. 2000-50887 or Japanese Patent Application Laid-Open (Kokai) No. 2001-21850 is an example of such enzymes, but the enzymes used in the present invention are not limited to them. A protein deamidating enzyme may be used, which has been prepared from the culture broth of a microorganism producing the protein deamidating enzyme. Microorganisms to be used for preparation of the protein deamidating enzyme are not particularly limited.

[0009] In order to prepare the protein deamidating enzyme from the culture broth of a microorganism, any known methods for protein separation and protein purification (centrifugation, UF concentration, salting-out, and various chromatographies using ion exchange resin or the like) may be used. For example, a culture broth can be centrifuged to remove the microorganism cells, followed by salting out, chromatography and the like in combination to obtain the target enzyme. In order to collect the intracellular enzyme from microbial cells, for example, the microbial cells are first subjected to pressurization, ultrasonic treatment or the like to crush, and the target enzyme is then separated and purified as described above. In this connection, a microorganism culture broth may be in advance subjected to filtration or centrifugation to collect the microorganism cells, which are subjected to a series of the above-mentioned steps (disrupt of the microbial cells, separation, purification of the enzyme). The enzyme may be powdered by drying step such as freeze drying, reduced-pressure drying, or the like, during which appropriate bulking agent (s) or drying aid(s) may be used.

[0010] Activity of a protein deamidating enzyme used in the present invention is determined by a modified method of the method described in Japanese Patent Application Laid-Open (Kokai) No. 2000-50887, namely, the following method: (1) 10 μ| of an aqueous solution containing the protein deamidating enzyme is added to 100 μΙοί 176 mM phosphate buffer (pH 6.5) containing 30 mM Z-GIn-Gly, and the reaction mixture is incubated at 37°C for 10 min, followed by adding 100 μΙ of 12% TCA solution thereto, whereby the reaction is terminated. (2) The resultant solution is diluted appropriately with 20 mM phosphate buffer (pH 6.0) to adjust an enzyme concentration to 0.05 mg/ml, and centrifuged (12000 rpm, 4°C, 5 min) to obtain a supernatant, which is analyzed to quantify NH3 by an F-kit ammonia (manufactured by Roche). (3) 10 μΙ of the supernatant and 190 μΙ of 0.1 M triethanolamine buffer (pH 8.0) are added to 100 μΙ of the reagent II solution (F-kit accessory), and left to stand at room temperature for 5 min. 100 μΙ of the resulting solution is used to determine the absorbance at 340 nm. The remaining 200 μΙ of the resulting solution is added with 1.0 μΙ of the reagent III (F-kit accessory, glutamate dehydrogenase), left to stand at room temperature for further 20 min, and then is used to determine the absorbance at 340 nm. The ammonia standard solution attached to the F-kit is used to make a calibration curve showing a relation between ammonia concentration and change in absorbance (340 nm), and the curve is used to determine the concentration of ammonia in the reaction solution. (4) The protein assay CBB (Coomassie Brilliant Blue) solution (manufactured by Nacalai Tesque) is used to determine a protein concentration at a detection wavelength of 595 nm. BSA (manufactured by Pierce) is used as the standard. (5) The activity of the protein deamidating enzyme is determined by the following equation:

Specific activity (U/mg) = (Concentration of ammonia in reaction solution (μπιοΐ/ml) x Reaction solution volume (ml) x Enzyme dilution rate) *· (Volume of enzyme solution (ml) x Protein concentration (mg/ml) x Reaction time (min)) [0011] The raw milk to be used according to the present invention denotes an edible milk such as cow milk, buffalo milk, goat milk, sheep milk, horse milk and the like. Further, a pasteurized milk, a milk formulated in component such as milk fat, a diluted milk, a concentrated milk, a dried milk, a defatted dry milk, a defatted milk solution, and a processed milk are included in this category.

[0012] The dairy product of the present invention denotes a solid or gel food produced using, as the raw material, a natural cheese, a processed cheese, a set yogurt, a stirred yogurt, a bavarois, a milk jelly, a pudding and the like.

[0013] The protein deamidating enzyme may be added to raw milk, alone or in combination with other raw material(s). The reaction conditions for the protein deamidating enzyme (such as enzyme amount, reaction time, temperature, pH of the reaction solution and the like) are not particularly limited, but the enzyme is added preferably in an amount of 0.1 to 500 units, more preferably 0.1 to 100 units per 1 L of the raw milk. In the case of a processed milk such as diluted milk, concentrated milk, dried milk, defatted dry milk or the like, the protein deamidating enzyme is used in an amount based on the volume in terms of a volume of the raw milk before processed. For example, in case that 100g of defatted dry milk is obtained from 1 L of the raw milk, 0.1 to 500 units of the enzyme per 100 g of the defatted dry milk that corresponds to 1 L of the raw milk are preferable and 0.1 to 100 units are more preferable. The reaction temperature is preferably 5-80°C, more preferably 20-60°C. The pH of the reaction solution is preferably 2-10, more preferably 4-8. The reaction time is preferably 10 sec to 48 hours, more preferably from 10 min to 24 hours. These conditions may be changed or adjusted appropriately depending on purity of the enzyme to use, kind and purity of protein to use, or the like. The solution after enzyme reaction may be, for example, heated to deactivate the enzyme in order to manufacture a dairy product, or may be subjected to no special deactivation in the same way as a rennet is.

[0014] The dairy product such as defatted dry milk or the like, which is improved in quality by adding raw milk with a protein deamidating enzyme to deamidate, may be added at a step for manufacturing other dairy products such as cheese, yogurt and the like. For example, a modified defatted dry milk, which is produced by adding 100 g of a defatted dry milk with 0. 1 to 500 units, preferably 20 to 100 units of the enzyme, can be added to raw milk at a rate of 1-5% to manufacture a yogurt which smoothness is imparted to.

Best Modes for Carrying Out the Invention [0015] Referring now to Examples, the present invention will be explained below in more detail.

Example 1 [0016] In the present example, a protein glutaminase derived from Chryseobacterium was used as the protein deamidating enzyme. The gene sequence of the protein glutaminase (EC.3.5.1) derived from Chryseobacterium proteolyticum strain has already been determined [Eur. J, Biochem.268,1410-1421 (2001)]. In view of the sequence, a gene sequence represented by SEQ ID NO: 1 was constructed wherein a codon was converted to a frequently used codon in Coryne-bacterium glutamicum. This sequence includes a signal sequence (pre-portion) and a pro-portion of the protein glutaminase, and a region for coding the mature protein glutaminase. The whole gene sequence was produced by synthesis. Based on the information about the gene sequence represented by SEQ ID NO: 1 thus constructed, primers having their respective sequences represented by SEQ ID NO: 5 (5’-CATGAAGAACCTTTTCCTGTC-3’) and SEQ ID NO: 6 (5’-GTAAAAGGATCCATTAATTAAAATCC-3’) were synthesized. The primer shown in SEQ ID NO: 5 included the N-terminal sequence of the signal sequence of the protein glutaminase, and the primer shown in SEQ ID NO: 6 included the C-terminal sequence of the mature protein glutaminase and the recognition sequence for BamH I. The DNA having sequence shown in SEQ. ID NO: 1 was used as the template, and the primers having their respective sequences shown in SEQ ID NO: 5 and SEQ ID NO: 6 were used to perform PCR, thereby to amplify the regions for coding the pro-portion of the protein glutaminase and the mature protein glutaminase. This PCR fragment was inserted into Smal site of pVC7 described in Japanese Patent Application Laid-Open (Kokai) No. Hei 9-070291 to give a plasmid, with which the competent cell of E. coli. JM109 (manufactured by Takara Shuzo) was then transduced to give a strain carrying the plasmid with the cloned protein glutaminase gene, thereby to collect the plasmid. The nucleic acid sequence of the fragment cloned in this plasmid was determined to confirm that it coincided with the sequence shown in SEQ ID NO: 1.

[0017] The sequence of TorA gene including TorA signal peptide derived from E. coli has already been determined (Mol. Microbiol. 11:1169-1179 (1994)). Referring to this sequence, the primers shown in SEQ ID NO: 7 (5-AT-GAACAATAACGATCTCTTTCAGG-3’) and in SEQ ID NO: 8 (5’-CCGGATCCTGGTCATGATTTCACCTG-3’) were synthesized, and the chromosomal DNA of E. co//'W3110 strain prepared according to an ordinary method (Method by Saitoh and Miura [Biochim. Biophys. Acta, 72, 619 (1963]) was used as the template to perform PCR, thereby to amplify the region for coding TorA and the region including the signal sequence located upstream. The PCR reaction was performed using Pyrobest DNA polymerase (manufactured by Takara Shuzo) under reaction conditions established according to the protocol recommended by the vendor. Meanwhile, the sequence shown in SEQ ID NO: 8 included a recognition sequence for restriction enzyme BamHI. The DNA sequence coding the signal sequence of TorA is shown in SEQ ID NO: 3.

[0018] Plasmid pPKSPTGI described in International Patent Publication WO 01/23591 was used as the template, and the primer having sequences shown in SEQ ID NO: 9 (5’-AAATTCCTGTGAATTAGCTGATTTAG-3’) and SEQ ID NO: 10 (5’-AAGAGATCGTTATTGTTCATAGAGGCGAAGGCTCCTTGAATAG-3’) were used to perform a PCR, thereby to amplify regions for coding the promoter and the signal peptide. The sequence shown in SEQ ID NO: 10 includes the 5’-terminal sequence of the gene for coding the TorA signal peptide. Next, this PCR product was mixed with the PCR product comprising a region containing a gene sequence for coding the TorA amplified by the primers having the sequences shown in SEQ ID NO: 7 and SEQ ID NO: 8 and a signal sequence located upstream thereof, at a ratio of 1:1 to give a mixture, which was used as a template to perform a cross-over PCR using the primers having sequences shown in SEQ ID NO: 8 and SEQ ID NO: 9. Through these manipulations, a fusion gene comprising a sequence including a PS2 promoter region, a TorA signal sequence and a sequence for coding the TorA was amplified. This cross-over PCR product was digested by restriction enzymes Seal and BamHI, and then subjected to agarose gel electrophoresis to detect an approximately 3.1 kbp DNA fragment. This DNA fragment was separated from the agarose gel, collected by EasyTrapVer. 2 (manufactured byTakaraShuzo),and inserted into the Scal-BamHI site of the plasmid pPK4 described in Japanese Patent Application Laid-Open (Kokai) No. Hei 9-322774 to give a plasmid pPKT-TorA. Determination of the nucleic acid sequence of the gene sequence inserted into this plasmid confirmed that the expected fusion gene had been constructed. This plasmid was used as the template, and primers having their respective sequences shown in SEQ ID NO: 9 and SEQ ID NO: 11 (5’-GATTTCCTGGTTGCCGTTGGAATCCGCAGTCGCACGTCGCGGCG-3’) were used to perform a PCR, thereby to amplify a portion including the promoter region of PS2 and the region for coding the TorA signal peptide. Meanwhile, the sequence shown in SEQ ID NO: 11 has the 5’-terminal sequence of the region for coding a protein deamidating enzyme with a pro-sequence. Next, a plasmid wherein a protein deamidating enzyme was cloned was used as the template, and primers having their respective sequences shown in SEQ ID NO: 6 and SEQ ID NO: 12 (5’-GATTCCAACGGCAACCAGGA-3’) were used to perform a PCR, thereby to amplify the region for coding the protein glutaminase with a pro-sequence. Further, these PCR products were mixed at a ratio of 1:1 to give a mixture, which was used as the template, and primers having their respective sequences shown in SEQ ID NO: 6 and SEQ ID NO: 9 were used to perform a cross-over PCR, thereby to amplify a fusion gene of the gene for coding the PS2 promoter region and the gene for coding the TorA signal sequence and the protein glutaminase with a pro-sequence. This PCR product was digested by restriction enzymes Seal and BamHI, and subjected to agarose gel electrophoresis to detect an approximately 3.1 kbp DNA fragment. This DNA fragment was separated from the agarose gel, collected using EasyTrapVer.2 (manufactured by Takara Shuzo), and inserted into Scal-BamHI site of the plasmid pPK4 described in the said Japanese Patent Application Laid-Open (Kokai) No. Hei 9-322774 to obtain a plasmid pPKT-PPG. Determination of the nucleic acid sequence of the inserted sequence in the plasmid confirmed that it was the expected fusion gene. The amino acid sequence of a protein glutaminase with a pro-sequence is shown in SEQ ID NO: 2 and the amino acid sequence of theTorA signal peptide is shown in SEQ ID NO: 4. However, it was anticipated thatthe amino acid sequence of a natural type protein glutaminase would be maturated by a commercially available protease to give no correctly cleaved pro-sequence. Then, "QTNK" in the C-terminal sequence of the pro-sequence was changed to "FGPK" so that the pro-sequence might be cleaved to get the same sequence as the N-terminal sequence of the natural type protein glutaminase. Primers having their respective sequences shown in SEQ ID NO: 13 (5’-CTT GGG GCC GAA GCC CTT GAC TTC TTT GGT CAG-3’) and SEQ ID NO: 14 (5’-TTC GGC CCC AAG TTG GCG TOO GTC ATT CCA GAT-3’) were used in order to change to "FGPK". The sequence shown in SEQ ID NO: 13 is a primer for amplifying the prosequence portion, and the sequence shown in SEQ ID NO: 14 is a primerfor amplifying the matured form portion. Using pPKT-PPG as the template, the primers having their respective sequences shown in SEQ ID NO: 12 and SEQ ID NO: 13 were used to amplify the pro-sequence portion of the protein glutaminase, and the primers having their respective sequences shown in SEQ ID NO: 14 and SEQ ID NO: 6 were used to amplify the matured form portion of the protein glutaminase. These PCR products were mixed at a ratio of 1:1 to give a mixture, which was used as the template, and the primer having their respective sequences shown in SEQ ID NO: 6 and SEQ ID NO: 12 were used to perform a crossover PCR, thereby to amplify the protein glutaminase gene with a pro-sequence wherein the C-terminal of the prosequence was changed to FGPK. This cross-over PCR product was cloned into the Smal site of pUC18 (pUCPPG (FGPK)) to confirm the nucleic acid sequence, indicating that the pro sequence had been changed. Next, an Aafll-BsfPI fragment (large) of pPKT-PPG and an Aafll-SsfPI fragment (small) of pUCPPG (FGPK), were ligated to construct pPKT-PPG (FGPK).

[0019] C. glutamicum ATCC13869 was transformed with the plasmid pPKT-PPG (FGPK), and incubated in CM2G agar culture medium containing 25 mg/l of kanamycin to select a transformant. The selected strain was incubated in the MM liquid culture containing 25 mg/l of kanamycin at 30°Cfor48 hours. The C. glutamicum culture broth was centrifuged to obtain a supernatant, which was then filtered off (0.45 μΐη). The filtrated solution was condensed using an ultrafiltration membrane (to exclude those having a molecular weight of 10,000 or less). The buffer was exchanged with 50 mM phosphate buffer (pH 7.5), and the pro-sequence portion of the protein deamidating enzyme was cleaved by trypsin to allow maturation. Then, the resultant solution was concentrated again, and the buffer was exchanged (20 mM acetate buffer, pH 5.0). The concentrated sample obtained was subjected to cation exchange chromatography to purify the active fraction of the protein deamidating enzyme. The activity per protein of the purified enzyme was analyzed according to the previously described method, and it was around 100-140 U/mg.

[0020] Full-fat milk was homogenized (pre-heated at 60°C, 30 kgf/cm2), pasteurized (72°C, 15 sec), and cooled to 31 °C to give a raw milk. 25 L thereof was divided into caldrons, added with a lactic acid bacterium starter (CHN-01: four-in-one mixture) (1% of the milk), and added with 2,10, and 50 units of the above-mentioned purified protein deamidating enzyme product (100 units/mg) per 1L of the raw milk. Further, 0.01% CaCI2, and 0.003% rennet were added to perform cutting (pH 6.2 on cutting). The resultant mass was added with hot water with light stirring, warmed (32°C), left to stand, and the whey was removed when the pH lowered down to 5.8. Further, the resultant curd was subjected to matting (set temperature 34°C, up to pH 5.2) and molding (reversed twice or three times every 30 min), left to stand overnight at 20°C (pH 5.2), and then cut into 125 g/piece. The pieces were salted (immersed in saturated NaCI solution for 3 min), dried (3 days at 5°C), vacuum-packed, and matured at 13°C. The product was molded, left to stand overnight to give a curd, the weight of which was measured to calculate a curd yield. The fresh cheese left for one week was subjected to sensory test. Results obtained are shown in Table 1.

[0021] As shown in Table 1, it has been revealed that protein deamidating enzyme treatment to raw milk improves a curd yield and allows production of cheese with smooth oral sensation and suppressed acidic taste.

Table 1: Curd Yeld and Results of Sensory Test on Fresh Cheese,

Example 2 [0022] 800 mLof commercially available low-temperature pasteurized milk was put in a jug, heated to 90°C with stirring on a hot-water bath, and cooled down to 48°C. The solution was added with 80 ml of a starter ("Danone" yogurt) and divided into every 100 ml, which was then added with the purified protein deamidating enzyme product (100 units/mg) prepared according to the method described in Example 1 by 1, 5, 10, 50, or 100 units per 1 L of the milk, stirred thoroughly, and divided into every approximately 20 mL in a container before it got cold. It was incubated in an incubator set to 48°C for 3-4 hours, and found to have a yogurt pH of 4.4 to 4.5, upon which it was stored in a refrigerator to terminate fermentation. It was stored overnight at 4°C, and, on the following day, analyzed using a texture analyzer to perform property determination and sensory test. Results are shown in Table 2.

[0023] As shown in Table 2, it has been revealed that the protein deamidating enzyme added to the raw milk allowed production of a yogurt with smooth oral sensation and suppressed acidic taste. Similarly, a method, wherein the protein deamidating enzyme was made to act on the raw milk at 50°C for 90 min, and then heated at 90°C for 5 min to be deactivated, followed by adding a starter and fermenting at 38°C to have a pH of 4.5, provided a yogurt with smooth oral sensation, especially very smooth oral sensation felt at the end of its eating.

Table 2: dH and Results of

Sensorv Test of Yoaurt

Example 3 [0024] 40 g of defatted dry milk (low heat; manufactured by National Federation of Dairy Cooperative Associations) was suspended in 800 ml of distilled water. The resultant suspension was added with 0.2 or 1 unit of the purified protein deamidating enzyme product (100 units/mg) prepared according to the method described in Example 1, per 1 g of the defatted dry milk (20 units or 100 units in terms of per 1 L of the raw milk), and subjected to reaction at 40°C for 2.5 hours, followed by heating to deactivate (at 65°C for 30 min; 1 hour for rising temperature). The deactivated mass was freeze-dried to prepare a modified defatted dry milk. Raw milk was added with this modified defatted dry milk at a rate of 1 to 5% of the raw milk, to prepare a yogurt according to the same method as in Example 2. The amount of 0.2 U/g was a condition for slightly deamidating the Gin in the defatted dry milk, and the amount of 1U/g was a condition for deamidating approximately 50% of the Gin which was able to be deamidated. As a control, an unmodified defatted dry milk was used.

[0025] Sensory test was carried out for each of the samples and it was found that the enzyme-untreated defatted dry milk could be added to provide a yogurt with increased solid content, thereby to furnish it with improved oral sensation and high-quality sense, compared with a yogurt with no defatted dry milk added. Meanwhile, the defatted dry milk, which was treated with 0.2 U/g of the protein deamidating enzyme, could be added to furnish a yogurt with smooth oral sensation that began at a rate of 1% of the raw milk and got remarkably effective at that of 3% or more. The defatted dry milk, which was treated with 1 U/g of the enzyme, could be added at a rate of 1% of the raw milk to furnish a yogurt with remarkable smooth oral sensation, retained hardness, and remarkably increased favorability.

Example 4 [0026] Full-fat milk was homogenized (pre-heated to 60°C, 30 kgf/cm2), pasteurized (75°C, 15 sec), and cooled to 31 °C to give a raw milk, 25 L of which was divided into caldrons, added with a lactic acid bacterium starter (CHN-01: four-in-one mixture) (1.4% relative to the milk), added with 2 or 10 units of the above-mentioned purified protein deamidating enzyme product (100 units/mg) per 1 L of the raw milk, and left to stand for 1 hour. Further, the resultant mass was added with 0.01% CaCI2 and 0.009% rennet, and confirmed 1 hour later to be coagulated to perform cutting (acidity 0.130, temperature 31 °C on cutting). After cutting, the resultant mass was removed of 1/3 volume whey, added with hot water with light stirring, warmed (35°C), left to stand (approximately 20 min), and removed of another 1/3 volume whey. The resultant mass was gradually added with hot water to reach 38°C, and stirred gently at the same temperature for 1 hour. Then, the resultant mass was squeezed in a vat at 38°C for about 30 min, and subjected to molding and cheese curd reverse. After 30 min of preliminary squeezing (3 kg/cm2), the resultant mass was reversed and substantially squeezed (5 kg/cm2), then immersed into water together with the mold to cool (10°C, overnight), salted (immersed into saturated saline solution for 4 hours), dried (at 12°C for 10 days), vacuum-packed, and matured at 12°C to produce a hard cheese. Evaluation of the cheese was performed upon curd production and after maturation. Results of evaluation upon cheese curd production are shown in Table 3, and the results of sensory test after 6 months of maturation are shown in Table 4.

[0027] As shown in Table 4, it has been revealed that protein deamidating enzyme can be added to raw milk to provide a matured cheese with smooth oral sensation and suppressed acidic taste and bitter taste.

Table 3: Results of Assessment upon cheese curd production

Table 4: Sensory Test of Cheese Curd after 6 Months of Maturation

Industrial Applicability [0028] According to the present invention, it is possible to provide a diary product with smooth oral sensation and suppressed acidic taste and bitter taste, and to manufacture cheese at improved curd yields. Therefore, the present invention is extremely useful in the industrial field of foods.

SEQUENCE LISTING

[0029] <110> Ajinomoto Co., Inc. <120 Dairy products and process for them <130 EPA-64307 <160 14 <170 Patently version 3.1

<210 1 <211> 963 <212> DNA <213> Chyrseobacterium proteolyticum <220> <221 > misc_feature <223> protein glutaminase; nucleotide sequence <400> 1 atgaagaacc ttttcctgtc catgatggcc ttcgtgaccg tcctcacctt caactcctgc 60 gccgattcca acggcaacca ggaaatcaac ggcaaggaga agctttccgt taacgattct 120 aagctgaagg atttcggcaa gaccgttccg gttggcatcg acgaagagaa cggcatgatc 180 aaggtgtcct tcatgttgac tgcgcagttc tacgagatca agccaaccaa ggaaaacgag 240 cagtacatcg gtatgcttcg ccaggctgtt aagaacgaat ctccagtcca cattttcctc 300 aagccaaaca gcaatgaaat cggcaaggtg gagtctgcat ccccagagga ogtccgctac 360 ttcaagacga tcctgaccaa agaagtcaag ggccagacca acaaattggc gtccgtcatt 420 ccagatgtgg ctaccctcaa ctctctcttc aaccaaatca agaaccagtc ttgcggtacc 480 tctacggcgt cctccccatg catcaccttc cgctacccag tcgacggctg ctacgcacgc 540 gcccacaaga tgcgccagat cttgatgaac aacggctatg actgtgagaa gcaattcgtg 600 tacggtaacc tcaaggcatc caccggcacc tgctgcgtgg cgtggagcta ccacgttgca 660 atcttggtga gctacaaaaa cgcttccggc gtgacggaaa aacgcattat tgatccatcc. 720 cttttitcca gcggtcctgt gaccgatacc gcatggcgca acgctfgcgt taacacctct 780 tgcggctctg catccgtttc ctcttacgct aacaccgcag gaaatgttta ttaccgctcc 840 ccatccaati cttacctgta tgacaacaat ctgatcaata ccaactgtgt cctgactaaa 900 ttctccctgc tttccggctg ttctccttca cctgcaccgg atgtctccag ctgtggattt 960 taa 963

<210 2 <211> 320 <212> PRT <213> Chyrseobacterium roteollyticum <220

<221 > MISC_FEATURE <223> protein glutaminase: amino acid sequence <400 2

Met Lys Asn Leu Phe Leu Ser Met Met Ala Phe Val Thr Val Leu Thr 15 10 .15 .

Phe Asn Ser Cys Ala Asp Ser Asn GIy Asn Gin Glu lie Asn Gly Lys 20 25 30

Glu Lys Leu Ser Val Asn Asp Ser Lys Leu Lys Asp Phe Gly Lys Thr 35 40 45

Val Pro Val Gly lie Asp Glu Glu Asn Gly Met lie Lys Val Ser Phe 50 55 60

Met Leu Thr Ala Gin Phe Tyr Glu lie Lys Pro Thr Lys Glu Asn Glu 65 70 75 80

Gin Tyr lie Gly Met Leu Arg Gin Ala Val Lys Asn Glu Ser Pro Val 85 90 95

His lie Phe Leu Lys Pro Asn Ser Asn Glu lie Gly Lys Val Glu Ser 100 105 110

Ala Ser Pro Glu Asp Val Arg Tyr Phe Lys Thr lie Leu Thr Lys Glu 115 120 125

Val Lys Gly Gin Thr Asn Lys Leu Ala Ser Val lie Pro Asp Val Ala 130 135 140

Thr Leu Asn Ser Leu Phe Asn Gin lie Lys Asn Gin Ser Cys Gly Thr 145 150 155 160

Ser Thr Ala Ser Ser Pro Cys lie Thr Phe Arg Tyr Pro Val Asp Gly 165 170 175

Cys Tyr Ala Arg Ala His Lys Met Arg Gin lie Leu Met Asn Asn Gly 180 185 190

Tyr Asp Cys Glu Lys Gin Phe Val Tyr Gly Asn Leu Lys Ala Ser Thr 195 200 205

Gly Thr Cys Cys Val Ala Trp Ser Tyr His Val Ala lie Leu Val Ser 210 215 220

Tyr Lys Ash Ala Ser Gly Val Thr Glu Lys Arg lie lie Asp Pro Ser 225 230 235 240

Leu Phe Ser Ser Gly Pro Val Thr Asp Thr Ala Trp Arg Asn Ala Cys 245 250 255

Val Asn Thr Ser Cys Gly Ser Ala Ser Val Ser Ser Tyr Ala Asn Thr 260 265 270

Ala Gly Asn Val Tyr Tyr Arg Ser Pro Ser Asn Ser Tyr Leu Tyr Asp 275 280 285

Asn Asn Leu 11e Asn Thr Asn Cys VaI Leu Thr Lys Phe Ser Leu Leu 290 295 300

Ser Gly Cys Ser Pro Ser Pro Ala Pro Asp Val Ser Ser Cys Gly Phe 305 310 315 320 <210 3 <211> 117

<212> DNA <213> Escherichia coli <220 <221 > misc_feature <223> TorA signal sequence: nucleotide sequence <400 3 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc 60 ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgacgtgc gactgcg 117 <210> 4 <211> 39

<212> PRT <213> Escherichia coli <220> <221 > MISC_FEATURE <223> TorA signal peptide <400> 4

Met Asn Asn Asn Asp Leu Phe Gin Ala Ser Arg Arg Arg Phe Leu Ala 1 5 10 15

Gin Leu Gly Gly Leu Thr Val Ala Gly Met Leu Gly Pro Ser Leu Leu 20 25 30

Thr Pro Arg Arg Ala Thr Ala 35

<210> 5 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 5 catgaagaac cttttcctgt c 21

<210> 6 <211 >26 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 6 gtaaaaggat ccattaatta aaatcc 26 <210> 7

<211> 25 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400 7 atgaacaata acgatctctt tcagg 25

<210> 8 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 8 ccggatcctg gtcatgattt cacctg 26

<210> 9 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 9 aaattcctgt gaattagctg atttag 26

<210> 10 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 10 aagagatcgt tattgttcat agaggcgaag gctccttgaa tag 43

<210> 11 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 11 gatttcctgg ttgccgttgg aatccgcagt cgcacgtcgc ggcg 44

<210> 12 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 12 gattccaacg gcaaccagga 20

<210> 13 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 13 cttggggccg aagcccttga cttctttggt cag 33

<210> 14 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> synthetic olygonucleotide <400> 14 ttcggcccca agttggcgtc cgtcattcca gat 33

Claims 1. A method for manufacturing a dairy product, wherein a protein deamidating enzyme is added to raw milk and allowed to act on the milk protein in the raw milk, wherein the protein deamidating enzyme acts directly on the amide groups of a protein and has an action to deamidate with neither peptide bond cleavage nor protein crosslink accompanied. 2. The method according to Claim 1, wherein said dairy product is cheese or yogurt. 3. The method according to Claims 1 or 2, wherein said protein deamidating enzyme is added in an amount of 0.1 to 500 units per 1 L of said raw milk. 4. A dairy product obtained by any one of Claims 1 to 3.

Patentansprüche 1. Verfahren zum Herstellen eines Molkereiprodukts, wobei ein Proteindeamidierungsenzym zu Ausgangsmilch gegeben wird und zugelassen wird, dass es auf das Milchprotein in der Ausgangsmilch wirkt, wobei das Proteindeamidierungsenzym direkt auf die Amidgruppen eines Protein wirkt und ohne gleichzeitige Peptidbindungsspaltung oder Proteinvernetzung eine Deamidierungwirkung zeigt. 2. Verfahren nach Anspruch 1, wobei das Molkereiprodukt Käse oder Joghurt ist. 3. Verfahren nach Anspruch 1 oder2, wobei das Proteindeamidierungsenzym in einer Menge von 0,1 bis 500 Einheiten pro ein Liter der Ausgangsmilch zugegeben wird. 4. Durch ein Verfahren nach einem der Ansprüche 1 bis 3 erhaltenes Molkereiprodukt.

Revendications 1. Procédé de fabrication d’un produit laitier, dans lequel une enzyme de désamidation des protéines est ajoutée à du lait cru puis on la laisse agir sur les protéines de lait dans le lait cru, où l’enzyme de désamidation des protéines agit directement sur les groupes amide d’une protéine et possède une action de désamidation sans clivage des liaisons peptidiques ni réticulation de la protéine associée. 2. Procédé selon la revendication 1, dans lequel ledit produit laitier est du fromage ou du yaourt. 3. Procédé selon les revendications 1 ou 2, dans lequel ladite enzyme de désamidation des protéines est ajoutée en une quantité de 0,1 à 500 unités pour 1 I dudit lait cru. 4. Produit laitier obtenu par l’une quelconque des revendications 1 à 3.

REFERENCES CITED IN THE DESCRIPTION

This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Patent documents cited in the description • JP HEI8173032 B [0003] · DE 2044866 A1 [0004] • JP HEI6197688 B [0003] · JP 2001120179 A [0005] • J P 2000050887 A [0003] [0008] [0010] · J P 2001021850 A [0008] • JP 2001218590 A [0003] · JP HEI9070291 B [0016] • JP 2003250460 A [0003] · WO 0123591 A [0018] • US 3796633 A [0004] · JP HEI9322774 B [0018]

Non-patent literature cited in the description • Eur. J, Biochem., 2001, vol. 268, 1410-1421 [0016] · SAITOH ; MIURA. Biochim. Biophys. Acta, 1963, vol. • Mol. Microbiol., 1994, vol. 11, 1169-1179 [0017] 72, 619 [0017]

Claims (2)

1. ί t : Tejtermék és isijárás stiftett ! :ÍEABáDMM|: IGÉNY PONTOK l·

   1. Elérés tejtermék előállításéra, amelyben egy fahéne-dezamidáiö enzimet | adunk nyerstejhez, és ezt a tejfehérjén a nyerstejhen hatni hagyjuk, ahol a fehérje'· dezamidálő enzim közvetlenül a fehérje amíd-csoportjain fejti ki hatását, és olyan dezarnfdélő hatással MnlelkeÉk, amely nem jár együtt sem peptídkőtés-hesitássai, sem pedig fehéíjedieresztkötés létrehozásával. Ϊ, Az 1. igénypont szerinti eljárás, ahol a tejtermék sajt vagy joghurt. S, Az 1 vagy 2. igénypont szerinti eljárás, ahol a fehérje dezsminálé enzimet 1 t nyerstejre vonatkoztatva 0,1-500 egységnyi mennyiségben adjuk hozzá,
2. 4, Az 1 '5, j|éhypeifgk epika szerinti eljárással előállított tejtemték: A meghatalmazott: í