HK1219978A1 - 在無血清懸浮細胞培養系統中生產重組慢病毒載體的可擴大的製造方法 - Google Patents

在無血清懸浮細胞培養系統中生產重組慢病毒載體的可擴大的製造方法

Info

Publication number
HK1219978A1
HK1219978A1 HK16107978.3A HK16107978A HK1219978A1 HK 1219978 A1 HK1219978 A1 HK 1219978A1 HK 16107978 A HK16107978 A HK 16107978A HK 1219978 A1 HK1219978 A1 HK 1219978A1
Authority
HK
Hong Kong
Prior art keywords
serum
manufacturing process
cell culture
culture system
lentiviral vectors
Prior art date
Application number
HK16107978.3A
Other languages
English (en)
Inventor
Guang Qu
John Fraser Wright
Original Assignee
Childrens Hospital Philadelphia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Childrens Hospital Philadelphia filed Critical Childrens Hospital Philadelphia
Publication of HK1219978A1 publication Critical patent/HK1219978A1/zh

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Manufacturing & Machinery (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
HK16107978.3A 2013-03-15 2016-07-08 在無血清懸浮細胞培養系統中生產重組慢病毒載體的可擴大的製造方法 HK1219978A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361787818P 2013-03-15 2013-03-15
PCT/US2014/030370 WO2014145578A1 (en) 2013-03-15 2014-03-17 Scalable manufacturing process to produce recombinant lentiviral vectors in serum-free suspension cell culture system

Publications (1)

Publication Number Publication Date
HK1219978A1 true HK1219978A1 (zh) 2017-04-21

Family

ID=51538029

Family Applications (1)

Application Number Title Priority Date Filing Date
HK16107978.3A HK1219978A1 (zh) 2013-03-15 2016-07-08 在無血清懸浮細胞培養系統中生產重組慢病毒載體的可擴大的製造方法

Country Status (17)

Country Link
US (2) US20140323556A1 (zh)
EP (1) EP2970920B1 (zh)
JP (1) JP6480910B6 (zh)
KR (1) KR102200278B1 (zh)
CN (1) CN105378074A (zh)
AU (1) AU2014232879B2 (zh)
BR (1) BR112015022292A2 (zh)
CA (1) CA2904366C (zh)
DK (1) DK2970920T3 (zh)
ES (1) ES2681434T3 (zh)
HK (1) HK1219978A1 (zh)
IL (1) IL241058B (zh)
MX (1) MX364690B (zh)
PT (1) PT2970920T (zh)
RU (1) RU2709678C2 (zh)
WO (1) WO2014145578A1 (zh)
ZA (1) ZA201506478B (zh)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11318163B2 (en) 2015-02-18 2022-05-03 Enlivex Therapeutics Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11304976B2 (en) 2015-02-18 2022-04-19 Enlivex Therapeutics Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11497767B2 (en) 2015-02-18 2022-11-15 Enlivex Therapeutics R&D Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11000548B2 (en) 2015-02-18 2021-05-11 Enlivex Therapeutics Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11512289B2 (en) 2015-02-18 2022-11-29 Enlivex Therapeutics Rdo Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11596652B2 (en) 2015-02-18 2023-03-07 Enlivex Therapeutics R&D Ltd Early apoptotic cells for use in treating sepsis
TWI547782B (zh) * 2015-03-16 2016-09-01 智易科技股份有限公司 可擴充式製程系統與實現該系統的方法
JP6803339B2 (ja) 2015-04-21 2020-12-23 エンリヴェックス セラピューティクス リミテッド 治療用のプールされた血液アポトーシス細胞調製物及びそれらの使用
BR112018011193A2 (pt) * 2015-12-01 2018-11-21 Spark Therapeutics Inc métodos escalonáveis para produzir vetor viral adeno-associado (aav) recombinante em sistema de cultura celular em suspensão isento de soro adequado para uso clínico
CN107043784A (zh) * 2016-02-05 2017-08-15 上海吉凯基因化学技术有限公司 一种慢病毒载体的制备方法
CN109069539A (zh) 2016-02-18 2018-12-21 恩立夫克治疗有限责任公司 用于癌症治疗的联合免疫疗法和细胞因子控制疗法
EP3436576A4 (en) * 2016-03-30 2019-10-30 Spark Therapeutics, Inc. CELL LINE FOR THE PRODUCTION OF RECOMBINANT PROTEIN AND / OR A RECOMBINANT VIRAL VECTOR
AU2017249402A1 (en) 2016-04-12 2018-10-04 Artemis Biosystems, Inc. Perfusion filtration systems
CN106497882A (zh) * 2016-10-29 2017-03-15 复旦大学 同时过表达R‑spondin1和Noggin的细胞株及其构建方法和应用
EP4382183A2 (en) 2017-03-06 2024-06-12 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
EP3420076B1 (en) 2017-03-06 2024-02-21 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
CN109251939A (zh) * 2017-07-14 2019-01-22 孙搏 Il-17g-197a慢病毒表达载体、其构建方法及慢病毒的包装测定方法
US20210155663A1 (en) * 2018-01-24 2021-05-27 Slbigen Inc. Mesenchymal stem cells expressing brain-derived neurotrophic factor and use thereof
CN111763238B (zh) * 2019-04-02 2022-07-29 普莱柯生物工程股份有限公司 一种蛋白抗原的纯化方法、制备的蛋白抗原及其应用
FI3953483T3 (fi) * 2019-04-11 2023-11-30 Regenxbio Inc Kokoekskluusiokromatografiamenetelmiä rekombinanttien adenoassosioituneiden viruskoostumusten karakterisoimiseksi
CN110054678B (zh) * 2019-05-16 2023-04-18 中国医学科学院血液病医院（血液学研究所） 一种膜结合型mFLT3LG蛋白及其应用
CN110894494B (zh) * 2019-11-22 2022-09-27 广西梧州制药（集团）股份有限公司 一种大规模高密度悬浮培养293细胞高产腺病毒的方法
CN110938654A (zh) * 2019-12-11 2020-03-31 和元生物技术（上海）股份有限公司 一种细胞转染试剂及其应用
CN113122501B (zh) * 2019-12-30 2023-06-16 重庆精准生物技术有限公司 适合大规模临床级病毒载体制备的培养基及其应用
CA3188923A1 (en) * 2020-08-13 2022-02-17 Michael Sean BURNHAM Methods for producing clinical-grade lentiviral vector
CN112746076B (zh) * 2020-12-28 2023-05-12 中吉智药(南京)生物技术有限公司 一种密码子优化的col7a1基因及慢病毒和应用
US20240101964A1 (en) * 2020-12-29 2024-03-28 Jiangsu Genscript Probio Biotech Co., Ltd. Hek293t cell strain having high dispersibility and screening method therefor
EP4373951A1 (en) * 2021-07-19 2024-05-29 2seventy bio, Inc. Vector manufacturing processes
CN113980916A (zh) * 2021-09-27 2022-01-28 湖南丰晖生物科技有限公司 一种慢病毒的纯化方法
WO2023061409A1 (zh) * 2021-10-12 2023-04-20 江苏金斯瑞蓬勃生物科技有限公司 适应无血清悬浮培养的hek293细胞株及其应用
CN114990077A (zh) * 2022-08-01 2022-09-02 深圳市先康达生命科学有限公司 一种慢病毒包装与纯化方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5869445A (en) * 1993-03-17 1999-02-09 University Of Washington Methods for eliciting or enhancing reactivity to HER-2/neu protein
US20030157070A1 (en) * 1994-12-30 2003-08-21 Jolly Douglas J. High efficiency ex vivo transduction of cells by high titer recombinant retroviral preparations
US6544769B1 (en) * 1996-12-13 2003-04-08 Schering Corporation Compostions comprising viruses and methods for concentrating virus preparations
US6207455B1 (en) 1997-05-01 2001-03-27 Lung-Ji Chang Lentiviral vectors
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US7575924B2 (en) * 2000-11-13 2009-08-18 Research Development Foundation Methods and compositions relating to improved lentiviral vectors and their applications
AU2002348151A1 (en) * 2001-11-05 2003-05-19 Genvec, Inc. Viral vector production methods and compositions
AU2003229159B2 (en) * 2002-04-30 2008-02-21 Oncolytics Biotech Inc. Improved viral purification methods
AU2003272868A1 (en) * 2003-10-15 2005-04-27 Agtc Gene Technology Company Ltd. Method for large-scale production, isolation, purification and the uses of multi-type recombinant adeno-associated virus vectors
RU2411081C2 (ru) * 2005-07-06 2011-02-10 Джи-И Хелткер Байо-Сайенсиз АБ Способ изготовления разделяющей матрицы
WO2008148304A1 (fr) * 2007-05-31 2008-12-11 Xiamen University Cible d'interférence arn pour traiter le sida
EP2012122A1 (en) * 2007-07-06 2009-01-07 Medigene AG Mutated parvovirus structural proteins as vaccines
JP5615271B2 (ja) 2008-06-18 2014-10-29 オックスフォード バイオメディカ（ユーケー）リミテッド ウイルス精製法
CN102216450B (zh) * 2008-09-24 2014-05-14 米迪缪尼有限公司 培养细胞、增殖和纯化病毒的方法
BRPI1015176B1 (pt) * 2009-06-16 2019-10-29 Genzyme Corp método para isolar uma população de partículas de vírus adenoassociado recombinante (raav) de impurezas de processo em uma corrente de alimentação
WO2011086509A1 (en) 2010-01-12 2011-07-21 Vascular Biogenics Ltd. Methods of producing adenovirus vectors and viral preparations generated thereby
WO2011097447A2 (en) * 2010-02-04 2011-08-11 Neurologix, Inc. Production of recombinant virus

Also Published As

Publication number Publication date
US10994028B2 (en) 2021-05-04
AU2014232879A1 (en) 2015-09-24
AU2014232879B2 (en) 2020-03-05
JP6480910B6 (ja) 2019-04-03
DK2970920T3 (en) 2018-08-06
US20140323556A1 (en) 2014-10-30
EP2970920A4 (en) 2016-09-14
BR112015022292A2 (pt) 2017-07-18
KR102200278B1 (ko) 2021-01-07
RU2709678C2 (ru) 2019-12-19
IL241058B (en) 2020-05-31
CA2904366C (en) 2023-07-04
CN105378074A (zh) 2016-03-02
JP2016512700A (ja) 2016-05-09
JP6480910B2 (ja) 2019-03-13
KR20160024841A (ko) 2016-03-07
IL241058A0 (en) 2015-11-30
ES2681434T3 (es) 2018-09-13
RU2015144057A (ru) 2017-04-27
MX2015012450A (es) 2016-04-25
US20170368201A1 (en) 2017-12-28
CA2904366A1 (en) 2014-09-18
MX364690B (es) 2019-05-06
WO2014145578A1 (en) 2014-09-18
EP2970920B1 (en) 2018-04-25
EP2970920A1 (en) 2016-01-20
ZA201506478B (en) 2021-09-29
PT2970920T (pt) 2018-07-30

Similar Documents

Publication Publication Date Title
HK1219978A1 (zh) 在無血清懸浮細胞培養系統中生產重組慢病毒載體的可擴大的製造方法
HRP20190484T1 (hr) Pripravci stanične kulture i postupci proizvodnje polipeptida
HK1222662A1 (zh) 體外生產具有可分選蛋白的無核紅細胞
HK1209651A1 (zh) 用於產生重組艾杜糖- -硫酸酯酶的細胞
IL273504A (en) Compositions for cell culture with antioxidants and methods for polypeptide production
EP2935581A4 (en) EXPRESSION VECTORS FOR THE PRODUCTION OF RECOMBINANT PROTEINS IN MAMMALIAN CELLS
HK1200848A1 (zh) 表達載體元件組合、新的生產用細胞產生方法及其在重組產生多肽中的用途
HUE059277T2 (hu) Eljárás elektrolízis cellában
SG11201403814RA (en) Production of recombinant proteins with simple glycoforms
HK1214455A1 (zh) 用於建立並保持小區集群的系統和方法
BR112014025693A2 (pt) método de produção recombinante de polipeptídeos em células procarióticas
HK1223979A1 (zh) 通過毒胡蘿蔔細胞懸浮培養生產毒胡蘿蔔素
GB201420277D0 (en) Cell culture system
SG11201600682PA (en) Expression vector for production of recombinant proteins in prokaryotic host cells
HK1221977A1 (zh) 用於產生供生產重組蛋白的哺乳動物生產細胞的物品和方法
HK1221742A1 (zh) 用於在原核細胞中重組生產多肽的方法
HK1203360A1 (zh) 工業大規模慢病毒載體生產系統以及由此生產的載體