GR930100040A - Βελτιωμένη οξειδωτική προσ?ήκη συζεύξεως δια σπεκτροφωτομετρική ανάλυση αναλυτών (χημικών και βιοχημικών συστατικών). - Google Patents

Βελτιωμένη οξειδωτική προσ?ήκη συζεύξεως δια σπεκτροφωτομετρική ανάλυση αναλυτών (χημικών και βιοχημικών συστατικών).

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Publication number
GR930100040A
GR930100040A GR930100040A GR930100040A GR930100040A GR 930100040 A GR930100040 A GR 930100040A GR 930100040 A GR930100040 A GR 930100040A GR 930100040 A GR930100040 A GR 930100040A GR 930100040 A GR930100040 A GR 930100040A
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Greece
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dye
mtbh
ans
produce
oxidizing agent
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GR930100040A
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GR1002975B (el
Inventor
S Yu Yeung
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Lifescan Inc
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Application filed by Lifescan Inc filed Critical Lifescan Inc
Publication of GR930100040A publication Critical patent/GR930100040A/el
Publication of GR1002975B publication Critical patent/GR1002975B/el

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/323-Methyl-2-benzothiazolinone hydrazone hydrochloride hydrate, i.e. MBTH
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/81Packaged device or kit

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

Μία συνεζευγμένη χρωστική, περιέχει 3-μεθυλ-2-βενζοθειαζολινονο υδραζόνη (ΜΤΒΗ) και 8-ανιλινο-1-ναφθαλινοσουλφονική ένωση (ANS), χρησιμοποιείται ως δείκτης σε μίασειρά αντιδράσεων που παράγουν ένα ισχυρό οξειδωτικόπαράγοντα όπως υπεροξείδιο του υδρογόνου ή άλλα υπεροξείδια ήυπερβορικές ενώσεις. Ο ισχυρός οξειδωτικός παράγων αντιδρά μετην συζυγική χρωστική δια να παραγάγει μία μπλέ χρωστικήένωση. Η συζυγιακή χρωστική ΜΤΒΗ-ANS παρουσιάζει ισχυρά καιεπίπεδο φασματική απορρόφηση στην περιοχή περίπου 600 έως 650νανομέτρα. Την περιοχή αυτή δεν την επηρεάζουν οι χρωστικέςτου αίματος και έτσι καθίσταται δυνατή μία μέτρηση τηςγλυκόζης και άλλων αναλυτών που αντιδρούν με το ένζυμοοξειδάσης προς παραγωγή του ισχυρού οξειδωτικού παράγοντοςδια χρησιμοποιήσεως οπτικών LTD επακριβώς άνευ πολλής οπτικήςκλιμακώσεως. Περαιτέρω τα ΜΤΒΗ και ANS είναι πολύ διαλυτά ειςτο υδατικό διάλυμα, αλλά καθίστανται αδιάλυτα μετά τηνοξειδωτική σύζευξη. Η μικρα διαλυτότης περιορίζει στοελάχιστον την εξασθένιση της χρωστικής και έτσι δίδει ένασταθερό τελικό σημείο.ω
GR930100040A 1992-02-03 1993-02-04 Βελτιωμενη οξειδωτικη προσθηκη συζευξεως δια σπεκτροφωτομετρικη αναλυση αναλυτων (χημικων και βιοχημικων συστατικων). GR1002975B (el)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US82965492A 1992-02-03 1992-02-03

Publications (2)

Publication Number Publication Date
GR930100040A true GR930100040A (el) 1993-10-29
GR1002975B GR1002975B (el) 1998-09-23

Family

ID=25255147

Family Applications (1)

Application Number Title Priority Date Filing Date
GR930100040A GR1002975B (el) 1992-02-03 1993-02-04 Βελτιωμενη οξειδωτικη προσθηκη συζευξεως δια σπεκτροφωτομετρικη αναλυση αναλυτων (χημικων και βιοχημικων συστατικων).

Country Status (10)

Country Link
US (1) US5453360A (el)
EP (1) EP0555045B1 (el)
JP (1) JP3342077B2 (el)
AT (1) ATE159052T1 (el)
AU (1) AU659525B2 (el)
CA (1) CA2088652C (el)
DE (1) DE69314363T2 (el)
DK (1) DK0555045T3 (el)
ES (1) ES2109429T3 (el)
GR (1) GR1002975B (el)

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Also Published As

Publication number Publication date
JPH0611447A (ja) 1994-01-21
AU659525B2 (en) 1995-05-18
ATE159052T1 (de) 1997-10-15
AU3219193A (en) 1993-08-05
GR1002975B (el) 1998-09-23
ES2109429T3 (es) 1998-01-16
DE69314363D1 (de) 1997-11-13
JP3342077B2 (ja) 2002-11-05
DE69314363T2 (de) 1998-02-19
EP0555045B1 (en) 1997-10-08
DK0555045T3 (da) 1998-03-16
EP0555045A1 (en) 1993-08-11
CA2088652A1 (en) 1993-08-04
US5453360A (en) 1995-09-26
CA2088652C (en) 2008-07-29

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