GB2026015A - Cell smear staining composition and material, and production and use thereof - Google Patents
Cell smear staining composition and material, and production and use thereof Download PDFInfo
- Publication number
- GB2026015A GB2026015A GB7925561A GB7925561A GB2026015A GB 2026015 A GB2026015 A GB 2026015A GB 7925561 A GB7925561 A GB 7925561A GB 7925561 A GB7925561 A GB 7925561A GB 2026015 A GB2026015 A GB 2026015A
- Authority
- GB
- United Kingdom
- Prior art keywords
- staining
- composition
- cell
- composition according
- dyestuff
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 34
- 238000010186 staining Methods 0.000 title claims abstract description 34
- 239000000463 material Substances 0.000 title claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 title description 2
- 239000000975 dye Substances 0.000 claims abstract description 14
- 229910052751 metal Inorganic materials 0.000 claims abstract description 10
- 239000002184 metal Substances 0.000 claims abstract description 10
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000012266 salt solution Substances 0.000 claims abstract description 8
- AAAQKTZKLRYKHR-UHFFFAOYSA-N triphenylmethane Chemical compound C1=CC=CC=C1C(C=1C=CC=CC=1)C1=CC=CC=C1 AAAQKTZKLRYKHR-UHFFFAOYSA-N 0.000 claims abstract description 7
- HLUCICHZHWJHLL-UHFFFAOYSA-N Haematein Natural products C12=CC=C(O)C(O)=C2OCC2(O)C1=C1C=C(O)C(=O)C=C1C2 HLUCICHZHWJHLL-UHFFFAOYSA-N 0.000 claims abstract description 6
- HNNSUZPWERIYIL-UHFFFAOYSA-N chembl1730100 Chemical compound O1CC2(O)CC3=CC(O)=C(O)C=C3C2=C2C1=C(O)C(=O)C=C2 HNNSUZPWERIYIL-UHFFFAOYSA-N 0.000 claims abstract description 6
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 15
- 239000002904 solvent Substances 0.000 claims description 4
- 238000007447 staining method Methods 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 13
- 210000004027 cell Anatomy 0.000 description 12
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 210000003855 cell nucleus Anatomy 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000002380 cytological effect Effects 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- AVFBYUADVDVJQL-UHFFFAOYSA-N phosphoric acid;trioxotungsten;hydrate Chemical compound O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O AVFBYUADVDVJQL-UHFFFAOYSA-N 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000012192 staining solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 description 1
- MPVDXIMFBOLMNW-ISLYRVAYSA-N 7-hydroxy-8-[(E)-phenyldiazenyl]naphthalene-1,3-disulfonic acid Chemical compound OC1=CC=C2C=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C2=C1\N=N\C1=CC=CC=C1 MPVDXIMFBOLMNW-ISLYRVAYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- SJEYSFABYSGQBG-UHFFFAOYSA-M Patent blue Chemical compound [Na+].C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=CC=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 SJEYSFABYSGQBG-UHFFFAOYSA-M 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- WLKAMFOFXYCYDK-UHFFFAOYSA-N [5-amino-4-[[3-[(2-amino-4-azaniumyl-5-methylphenyl)diazenyl]-4-methylphenyl]diazenyl]-2-methylphenyl]azanium;dichloride Chemical compound [Cl-].[Cl-].CC1=CC=C(N=NC=2C(=CC([NH3+])=C(C)C=2)N)C=C1N=NC1=CC(C)=C([NH3+])C=C1N WLKAMFOFXYCYDK-UHFFFAOYSA-N 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 159000000013 aluminium salts Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001621 bismuth Chemical class 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 150000001879 copper Chemical class 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- JBBPTUVOZCXCSU-UHFFFAOYSA-L dipotassium;2',4',5',7'-tetrabromo-4,7-dichloro-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate Chemical compound [K+].[K+].O1C(=O)C(C(=CC=C2Cl)Cl)=C2C21C1=CC(Br)=C([O-])C(Br)=C1OC1=C(Br)C([O-])=C(Br)C=C21 JBBPTUVOZCXCSU-UHFFFAOYSA-L 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- IINNWAYUJNWZRM-UHFFFAOYSA-L erythrosin B Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(I)C(=O)C(I)=C2OC2=C(I)C([O-])=C(I)C=C21 IINNWAYUJNWZRM-UHFFFAOYSA-L 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 159000000014 iron salts Chemical class 0.000 description 1
- -1 light green Chemical compound 0.000 description 1
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 1
- 229910052808 lithium carbonate Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009595 pap smear Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- XOSXWYQMOYSSKB-LDKJGXKFSA-L water blue Chemical compound CC1=CC(/C(\C(C=C2)=CC=C2NC(C=C2)=CC=C2S([O-])(=O)=O)=C(\C=C2)/C=C/C\2=N\C(C=C2)=CC=C2S([O-])(=O)=O)=CC(S(O)(=O)=O)=C1N.[Na+].[Na+] XOSXWYQMOYSSKB-LDKJGXKFSA-L 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The composition comprises haematein, a triphenylmethane dyestuff (preferably light green) and a fluorescein dyestuff (preferably eosin). The composition is preferably in the form of a dry, homogeneous (preferably solution-applied) film coated on a support, so as to constitute a cell smear staining material. The composition and material are used for staining cell smears (for cytodiagnosis) in a single staining step by mordanting the smears with a metal salt solution and then contacting the smears with the composition. This staining method is simple and (when the cell staining material is used) clean.
Description
SPECIFICATION
Cell smear staining composition and material, and production and use thereof
The invention relates to cell smear staining. For decades (more than 30 years) the standard method of staining cell smears in gynaecological cytodiagnosis for the early recognition of genital cancer in women has been the Papanicolaou stain. The principle of this method is that vaginal, portio and cervical smears, and also uterine sediment smears, which contain individual cells or cell groupings, are stained with several dyestuff solutions. On the basis of cell morphology, cancer cells or precursors thereof can be recognised.
The disadvantages of this staining method are that it is very time-consuming and many years' experience are required in order to be able to evaluate the results obtained. The Papanicolaou stain method involves 23-25 steps, including covering of the preparation; the time taken is about 30-45 minutes. The actual staining procedure involves more than 10 steps (3 staining solutions and rinsing with water, alcohol, acid and base). Attempts have been made to simplify the time-consuming staining operation and to shorten the time taken so that gynaecologists could use the method in their own practices without having to send smears to an outside, specialist cytological laboratory.
A large number of modifications and rapid staining techniques have been proposed for the
Papanicolaou stain, but have only achieved limited acceptance. Papanicolaou was aware of the disadvantages of his method and he described in J. Lab. Clin. Med., 1941, page 1,200, a staining solution consisting of aniline blue, orange G, fuchsin, eosin, molybdatophosphoric acid and tungstophosphoric acid, which is said to provide a stain usable for many cases in only 3 minutes; however, without the use of haematoxylin, the nuclei are not stained so well and cytological details are not so easily discernible. Papanicolaou gave a similar report on his solutions EA 36 and EA 25, which consist of light green, Bismarck brown, eosin, tungstophosphoric acid and lithium carbonate (Science, 95, 2469 (1942)). In ann. Biol.Clin., 12, 187 (1954), the Papanicolaou stain is compared with seven other methods, showing that although single stains stain the cytoplasm very well, they are not able to show the structure of the nucleus. In every case, at least an additional stain with haematoxylin is necessary.
We have now developed a staining composition with which it is possible, in a single staining step, to obtain images which correspond to the Papanicolaou stains, both in respect of the fine structure and in respect of the colour character.
According to the invention, there is provided a composition for staining cell smears, which comprises haematein, a triphenylmethane dyestuff (preferably light green) and a fluorescein dyestuff (preferably eosin).
The composition may be in the form of a solution which preferably contains 0.01-0.5% by weight of haematein, 0.01-0.5% by weight of the triphenylmethane duestuff and 0.05-1% by weight of the fluorescein dyestuff. Such a solution may be used directly for staining cell smears, but since solutions generally do not have unlimited stability and their composition can change if they are used repeatedly, for example, as a result of partial evaporation of the solvent or due to crystallisation of a constituent, it is advantageous to apply these solutions to a support and to evaporate the solvent to form a dry homogeneous film of the composition according to the invention. Suitable supports for such coatings are, for example, glass, plastics films or absorbent supports, such as paper or glass fibre paper.
The resulting coated material is particularly useful for staining cell smears, since the staining can be carried out cleanly and simply without the need to store dyestuff solutions with its associated difficulties.
The composition according to the invention is preferably used in a process of staining smear samples present on a sample-receiving surface (such as, for example, a microscope slide), as follows.
The smear samples should be fixed immediately in conventional manner, for example, by spraying with a commercially available fixative or by dipping in an ethanol/ether mixture.
In order that the cell nucleus should be stained, the fixed smear should be mordanted. For this purpose, the smear sample is treated with a metal salt solution, for about 3 minutes, for example. Suitable metal salts include, for example, iron salts, bismuth salts, copper salts and aluminium salts, the latter being preferred. Preferably, a complexing agent, such as citric acid, tartaric acid, gluconic acid, ethylenediaminetetraacetic acid or the corresponding salts, is added to the metal salt solution so as to reduce or avoid the formation of precipitates. The solution preferably contains the metal salt in a concentration of about 0.1 -5% by weight. With lower concentrations, the nucleus is stained hardly any more deeply than the plasma and with high salt concentrations coloured precipitates are likely to be formed in the plasma.
The treated smear samples are then contacted with a composition according to the invention, which may be in the form of a solution or dry (for example, when present as a coating on a support). When the composition is applied dry, the samples should still be moist following treatment with the metal salt solution. A suitable time for contact with the composition according to the invention is about 5 minutes.
When the composition according to the invention is used in the form of a coating on a substrate, the substrate can be removed subsequently, if desired. The stained smear is then preferably rinsed with alcohol and covered.
With the preferred composition according to the invention (that is a composition comprising haematein, light green, and eosin in the preferred proportions indicated above), the colour pattern of the resulting stains corresponds closely to that of Papanicolaou stains. Slight colour shifts can arise if the ratios of dyestuffs to one another are changed or if, for example, phloxin or erythrosin is used in piace of the preferred fluorescein dyestuff, eosin, or if, for example, patent blue is used in plalce of the preferred triphenylmethane dyestuff, light green.
In order that the invention may be more fully understood, the following Examples are given by way of illustration only:
Example A
Preparation of the solutions
1. Metal salt solution
0.5 g of aluminium chloride and 0.5 g of sodium citrate in 100 ml of water
2. Staining composition
The following 3 solutions are prepared and filtered:
(a) 1.20 g of haematein in 300 ml of ethanol
(b) 0.69 g of light green in 20 ml of water and 100 ml of ethanol
(c) 2.88 g of eosin in 30 ml of water and 100 ml of ethanol.
30 ml of solution (a), 1 2 ml of solution (b) and 1 3 ml of solution (c) are combined to give a staining composition.
Example B
Preparation of a cell smear staining material
The staining composition prepared according to Example A2 is applied uniformly to a 55 mm wide and 1,000 mm long film of glass-clear polyvinyl chloride (thickness 0.1 5 mm), for example by applying with a sponge or by spraying or printing. After the solvent has evaporated, the film is cut into strips 24 mm wide and can then be used for staining.
Example C
Staining procedure
A vaginal, cervical or uterine smear on a microscope slide is fixed immediately by placing the slide in a 1:1 mixture of ethanol/ether for about 10-15 minutes. The smear can also be fixed by spraying with a fixative spray. The smear fixed in this way is dipped in the metal salt solution described in Example Al for about 3 minutes. The staining material prepared according to
Example B is placed on the smear, whilst the latter is still moist. After 5 minutes the film is removed and the smear is rinsed with alcohol and covered in conventionai manner.
The cell constituents of the smear are observed in the microscopic image to be stained as follows:
Basophilic cytoplasm blue-green
Acidophilic cytoplasm pink
Erythrocytes red
Cornified plasma pink to orange
Cell nuclei blue to dark violet
Claims (14)
1. A composition for staining cell smears, which comprises haematin, a triphenylmethane dyestuff and a fluorescein dyestuff.
2. A composition according to claim 1, in which the triphenylmethane duestuff is light green.
3. A composition according to claim 1 to 2, in which the fluorescien dyestuff is eosin.
4. A composition according to any of claims 1 to 3, which is in the form of a solution containing 0.01-0.5% by weight of haematein, 0.01-0.5% by weight of the triphenylmethane dyestuff and 0.05-1% by weight of the fluorescein dyestuff.
5. A composition for staining cell smears, substantially as herein described in Example A.
6. A cell smear staining material, which comprises a support having thereon a dry homogeneous film of a composition according to any of claims 1 to 3.
7. A cell smear staining material, substantially as herein described in Example B.
8. A method of producing a cell smear staining material, which comprises applying a solution of a composition according to any of claims 1 to 3 to a support and removing the solvent to form a dry homogeneous film of said composition on the support.
9. A method according to claim 8, in which the solution is as defined in claim 4.
10. A cell smear staining material, when produced by a method according to claim 8 or 9.
11. A process of staining fixed cell smear samples present on a sample-receiving surface, which comprises mordanting the samples with a metal salt solution and then contacting the samples, while they are still moist, with a composition according to any one of claims 1 to 3 or with the dry homogeneous film of a material according to claim 6, 7 or 10.
12. A process of staining fixed cell smear samples present on sample-receiving surfaces, which comprises treating the samples with a metal salt solution and contacting the samples with a composition according to claim 4 or 5.
1 3. A process of staining cell smears, substantially as herein described in Example C.
14. For use in cytodiagnosis, a composition according to any of claims 1 to 5 or a material according to claim 6, 7 or 10.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19782832491 DE2832491A1 (en) | 1978-07-24 | 1978-07-24 | MEANS AND METHOD FOR STAINING CELL SWABS |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB2026015A true GB2026015A (en) | 1980-01-30 |
| GB2026015B GB2026015B (en) | 1982-08-18 |
Family
ID=6045264
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB7925561A Expired GB2026015B (en) | 1978-07-24 | 1979-07-23 | Cell smear staining composition and material and production and use thereof |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS5518999A (en) |
| DE (1) | DE2832491A1 (en) |
| FI (1) | FI792304A (en) |
| FR (1) | FR2434394A1 (en) |
| GB (1) | GB2026015B (en) |
| IT (1) | IT1118123B (en) |
| SE (1) | SE7906296L (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2535461A1 (en) * | 1982-10-28 | 1984-05-04 | Sclavo Spa | REAGENT FOR THE DETERMINATION OF B-LIPOPROTEINS |
| US5891733A (en) * | 1994-10-20 | 1999-04-06 | Toa Medical Electronics Co., Ltd. | Reagent for analyzing solid components in urine and method for analyzing solid components by employing the same |
| WO2004035091A1 (en) * | 2002-10-14 | 2004-04-29 | Fluoron Gmbh | Production of a dye for colouring cells in the human or animal body |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4487839A (en) * | 1983-01-05 | 1984-12-11 | Ortho Diagnostic Systems Inc. | Immunoassay methods employing patterns for the detection of soluble and cell surface antigens |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH294394A (en) * | 1950-05-02 | 1953-11-15 | Inc Technicon Chemical Company | Coloring liquid for animal tissue. |
| DE1044459B (en) * | 1956-11-15 | 1958-11-20 | Merck Ag E | Process for dyeing fiber materials for the purpose of carrying out fiber material analyzes and the means used for this purpose |
| DE1617641A1 (en) * | 1967-06-16 | 1971-03-25 | Merck Patent Gmbh | Dye preparations for coloring blood preparations and processes for their production |
| IT966513B (en) * | 1970-10-30 | 1974-02-20 | Gen Electric | PRE-COLORED SLIDES FOR BLOOD TESTS |
-
1978
- 1978-07-24 DE DE19782832491 patent/DE2832491A1/en not_active Withdrawn
-
1979
- 1979-07-19 FR FR7918692A patent/FR2434394A1/en not_active Withdrawn
- 1979-07-23 FI FI792304A patent/FI792304A/en not_active Application Discontinuation
- 1979-07-23 GB GB7925561A patent/GB2026015B/en not_active Expired
- 1979-07-23 SE SE7906296A patent/SE7906296L/en not_active Application Discontinuation
- 1979-07-23 IT IT49831/79A patent/IT1118123B/en active
- 1979-07-24 JP JP9332779A patent/JPS5518999A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2535461A1 (en) * | 1982-10-28 | 1984-05-04 | Sclavo Spa | REAGENT FOR THE DETERMINATION OF B-LIPOPROTEINS |
| GB2129129A (en) * | 1982-10-28 | 1984-05-10 | Sclavo Spa | Reagent for determining beta -lipoproteins |
| US5891733A (en) * | 1994-10-20 | 1999-04-06 | Toa Medical Electronics Co., Ltd. | Reagent for analyzing solid components in urine and method for analyzing solid components by employing the same |
| WO2004035091A1 (en) * | 2002-10-14 | 2004-04-29 | Fluoron Gmbh | Production of a dye for colouring cells in the human or animal body |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2026015B (en) | 1982-08-18 |
| IT7949831A0 (en) | 1979-07-23 |
| FR2434394A1 (en) | 1980-03-21 |
| SE7906296L (en) | 1980-01-26 |
| FI792304A (en) | 1980-01-25 |
| DE2832491A1 (en) | 1980-02-07 |
| JPS5518999A (en) | 1980-02-09 |
| IT1118123B (en) | 1986-02-24 |
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