FR2429837A1 - Procede de preparation pure d'urokinase - Google Patents
Procede de preparation pure d'urokinaseInfo
- Publication number
- FR2429837A1 FR2429837A1 FR7916757A FR7916757A FR2429837A1 FR 2429837 A1 FR2429837 A1 FR 2429837A1 FR 7916757 A FR7916757 A FR 7916757A FR 7916757 A FR7916757 A FR 7916757A FR 2429837 A1 FR2429837 A1 FR 2429837A1
- Authority
- FR
- France
- Prior art keywords
- urokinase
- pure
- pure preparation
- solution
- matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/815—Enzyme separation or purification by sorption
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
La présente invention concerne un procédé de préparation pure et de concentration d'urokinase en partant d'une solution brute d'urokinase. La solution d'urokinase est mise au contact d'une matrice en substance solide poreuse à surface spécifique élevée, dans un milieu à pH >= 6. Sur la surface de la matrice on a immobilisé de l'aprotinine au moyen de composés chimiques covalents. Ensuite, on traite l'urokinase adsorbée par élution à pH =< 4,5, à l'aide d'un agent d'élution. Les solutions pures et très concentrées d'urokinase sont utilisées en médecine comme produit pour dissoudre les caillots de fibrine.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH719778A CH643297A5 (de) | 1978-06-30 | 1978-06-30 | Verfahren zur reindarstellung von urokinase. |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2429837A1 true FR2429837A1 (fr) | 1980-01-25 |
FR2429837B1 FR2429837B1 (fr) | 1984-01-20 |
Family
ID=4321401
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7916757A Granted FR2429837A1 (fr) | 1978-06-30 | 1979-06-28 | Procede de preparation pure d'urokinase |
Country Status (10)
Country | Link |
---|---|
US (1) | US4259447A (fr) |
JP (1) | JPS5832591B2 (fr) |
AR (1) | AR220202A1 (fr) |
CH (1) | CH643297A5 (fr) |
DE (1) | DE2924744C2 (fr) |
FR (1) | FR2429837A1 (fr) |
GB (1) | GB2025977B (fr) |
IL (1) | IL57596A (fr) |
IT (1) | IT1121914B (fr) |
NL (2) | NL185573C (fr) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2121050B (en) * | 1979-07-05 | 1986-03-26 | Genentech Inc | Preparation of functional human urokinase proteins |
NL8003402A (nl) * | 1980-06-11 | 1982-01-04 | Leuven Res & Dev Vzw | Nieuwe plasminogeen-activator en farmaceutisch preparaat met trombolytische werking. |
US5112755A (en) * | 1982-04-15 | 1992-05-12 | Genentech, Inc. | Preparation of functional human urokinase proteins |
US4766075A (en) * | 1982-07-14 | 1988-08-23 | Genentech, Inc. | Human tissue plasminogen activator |
US5185259A (en) * | 1982-05-05 | 1993-02-09 | Genentech, Inc. | Truncated human tissue plasminogen activator |
FI831484L (fi) * | 1982-05-05 | 1983-11-06 | Genentech Inc | Plasminogen aktivator foer maenskovaevnad |
SU1662352A3 (ru) * | 1982-05-05 | 1991-07-07 | Генентек, Инк (Фирма) | Штамм бактерий ЕSснеRIснIа coLI - продуцент активатора плазминогена тканевого типа |
US4853330A (en) * | 1983-04-07 | 1989-08-01 | Genentech, Inc. | Human tissue plasminogen activator |
US4525465A (en) * | 1983-10-07 | 1985-06-25 | Nippon Kayaku Kabushiki Kaisha | Water-insoluble biospecific absorbent containing argininal derivative |
DE3750664T2 (de) * | 1986-07-15 | 1995-06-22 | Kowa Co | Throminbindende Substanz und Verfahren zu ihrer Herstellung. |
US4920051A (en) * | 1988-02-03 | 1990-04-24 | Damon Biotech, Inc. | Recovery of urokinase compounds |
JP2002267642A (ja) * | 2001-03-13 | 2002-09-18 | Tosoh Corp | アルカリ土類金属イオン測定用イオンクロマトグラフィー溶離液およびそれを用いたアルカリ土類金属イオン分析方法 |
EP3307070A4 (fr) | 2015-06-12 | 2019-01-02 | Emory University | Compositions de croissance et de survie pour des cellules capables de produire des anticorps et procédés associés |
US11125757B2 (en) | 2017-05-26 | 2021-09-21 | Emory University | Methods of culturing and characterizing antibody secreting cells |
KR102277471B1 (ko) | 2019-10-22 | 2021-07-14 | 주식회사 지니스 | '혈관내 혈전' 용해제 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52105279A (en) * | 1976-03-01 | 1977-09-03 | Wakamoto Pharma Co Ltd | Collfcting method of urokinase and novel absorbing agent used in said method |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3010074A (en) * | 1959-02-25 | 1961-11-21 | Raytheon Co | Adjustable core transformer oscillator |
US4165258A (en) * | 1975-10-08 | 1979-08-21 | University Of Pennsylvania | Plasminogen activating enzyme-specific competitive inhibitor |
DE2632221A1 (de) * | 1976-07-16 | 1978-01-19 | Frantisek Zdobinsky | Diebstahlsicherungstafel fuer kraftfahrzeuge und fahrraeder |
US4066506A (en) * | 1976-10-08 | 1978-01-03 | The United States Of America As Represented By The Secretary Of Health, Education And Welfare | Method of separating and purifying two active forms of urokinase using affinity chromatography |
-
1978
- 1978-06-30 CH CH719778A patent/CH643297A5/de not_active IP Right Cessation
-
1979
- 1979-06-18 AR AR276951A patent/AR220202A1/es active
- 1979-06-19 IL IL57596A patent/IL57596A/xx unknown
- 1979-06-20 DE DE2924744A patent/DE2924744C2/de not_active Expired
- 1979-06-25 IT IT23835/79A patent/IT1121914B/it active Protection Beyond IP Right Term
- 1979-06-28 FR FR7916757A patent/FR2429837A1/fr active Granted
- 1979-06-28 NL NLAANVRAGE7905045,A patent/NL185573C/xx not_active IP Right Cessation
- 1979-06-28 GB GB7922471A patent/GB2025977B/en not_active Expired
- 1979-06-29 US US06/053,552 patent/US4259447A/en not_active Expired - Lifetime
- 1979-06-29 JP JP54082534A patent/JPS5832591B2/ja not_active Expired
-
1993
- 1993-06-24 NL NL930096C patent/NL930096I1/nl unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52105279A (en) * | 1976-03-01 | 1977-09-03 | Wakamoto Pharma Co Ltd | Collfcting method of urokinase and novel absorbing agent used in said method |
Non-Patent Citations (3)
Title |
---|
CA1975 * |
CA1976 * |
CA1977 * |
Also Published As
Publication number | Publication date |
---|---|
IL57596A (en) | 1982-12-31 |
DE2924744A1 (de) | 1980-01-17 |
GB2025977B (en) | 1982-09-29 |
NL7905045A (nl) | 1980-01-03 |
CH643297A5 (de) | 1984-05-30 |
JPS5523993A (en) | 1980-02-20 |
DE2924744C2 (de) | 1982-09-16 |
IT7923835A0 (it) | 1979-06-25 |
IT1121914B (it) | 1986-04-23 |
JPS5832591B2 (ja) | 1983-07-14 |
NL185573B (nl) | 1989-12-18 |
GB2025977A (en) | 1980-01-30 |
IL57596A0 (en) | 1979-10-31 |
NL930096I1 (nl) | 1993-10-01 |
AR220202A1 (es) | 1980-10-15 |
FR2429837B1 (fr) | 1984-01-20 |
NL185573C (nl) | 1990-05-16 |
US4259447A (en) | 1981-03-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
TP | Transmission of property | ||
CC | Supplementary protection certificate (spc) laid open to the public (law of 25 june 1990) |
Free format text: 92C0252, 920521 |
|
CB | Supplementary protection certificate (spc) granted (law of 25 june 1990) |
Free format text: 92C0251, 790628 |
|
CB | Supplementary protection certificate (spc) granted (law of 25 june 1990) |
Free format text: 92C0252, 790628 |
|
CL | Concession to grant licences |