FR2368497A1 - PROCESS FOR SELECTIVE INACTIVATION OF PROTEASE IN A PREPARATION OF BACTERIAL A-AMYLASE, PREPARATION OF ENZYME OBTAINED AND APPLICATION TO HYDROLYSIS OF A STARCHLACE MATERIAL - Google Patents

PROCESS FOR SELECTIVE INACTIVATION OF PROTEASE IN A PREPARATION OF BACTERIAL A-AMYLASE, PREPARATION OF ENZYME OBTAINED AND APPLICATION TO HYDROLYSIS OF A STARCHLACE MATERIAL

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Publication number
FR2368497A1
FR2368497A1 FR7724110A FR7724110A FR2368497A1 FR 2368497 A1 FR2368497 A1 FR 2368497A1 FR 7724110 A FR7724110 A FR 7724110A FR 7724110 A FR7724110 A FR 7724110A FR 2368497 A1 FR2368497 A1 FR 2368497A1
Authority
FR
France
Prior art keywords
preparation
amylase
hydrolysis
protease
application
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7724110A
Other languages
French (fr)
Other versions
FR2368497B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever Bestfoods North America
Original Assignee
Unilever Bestfoods North America
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/712,103 external-priority patent/US4235970A/en
Application filed by Unilever Bestfoods North America filed Critical Unilever Bestfoods North America
Publication of FR2368497A1 publication Critical patent/FR2368497A1/en
Application granted granted Critical
Publication of FR2368497B1 publication Critical patent/FR2368497B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Emergency Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention décrit l'inactivation sélective de l'activité protéolytique constituant une impureté dans des préparations d' alpha -amylase, et l'application à la transformation par hydrolyse d'une matière amylacée. On effectue cette inactivation par un traitement de chauffage modéré en présence d'une matière protectrice (calcium et/ou hydrolysats d'amidon comme du sirop de glucose). L'aamylase sans protéase peut alors servir à solubiliser des matières amylacées dans divers procédés classiques ou traitant de l'amidon granulaire. Les hydrolysats obtenus contiennent nettement moins de protéines solubles que ceux préparés à l'aide d' alpha -amylases non traitées. Une préparation d' alpha -amylase préférée est celle provenant du micro-organisme Bacillus licheniformis.The invention describes the selective inactivation of the proteolytic activity constituting an impurity in alpha-amylase preparations, and application to the transformation by hydrolysis of a starchy material. This inactivation is carried out by a moderate heating treatment in the presence of a protective material (calcium and / or starch hydrolysates such as glucose syrup). The non-protease amylase can then be used to solubilize starchy materials in various conventional or granular starch processing methods. The hydrolysates obtained contain markedly less soluble protein than those prepared using untreated alpha -amylases. A preferred alpha-amylase preparation is that from the microorganism Bacillus licheniformis.

FR7724110A 1976-08-05 1977-08-04 PROCESS FOR SELECTIVE INACTIVATION OF PROTEASE IN A PREPARATION OF BACTERIAL A-AMYLASE, PREPARATION OF ENZYME OBTAINED AND APPLICATION TO HYDROLYSIS OF A STARCHLACE MATERIAL Granted FR2368497A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71210276A 1976-08-05 1976-08-05
US05/712,103 US4235970A (en) 1976-08-05 1976-08-05 Protease inactivated α-amylase preparations

Publications (2)

Publication Number Publication Date
FR2368497A1 true FR2368497A1 (en) 1978-05-19
FR2368497B1 FR2368497B1 (en) 1982-12-17

Family

ID=27108759

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7724110A Granted FR2368497A1 (en) 1976-08-05 1977-08-04 PROCESS FOR SELECTIVE INACTIVATION OF PROTEASE IN A PREPARATION OF BACTERIAL A-AMYLASE, PREPARATION OF ENZYME OBTAINED AND APPLICATION TO HYDROLYSIS OF A STARCHLACE MATERIAL

Country Status (13)

Country Link
JP (1) JPS5344688A (en)
AR (1) AR219710A1 (en)
AU (1) AU515262B2 (en)
BR (1) BR7705171A (en)
DE (1) DE2735480C2 (en)
DK (1) DK350177A (en)
ES (1) ES461346A1 (en)
FR (1) FR2368497A1 (en)
GB (1) GB1546556A (en)
IT (1) IT1085406B (en)
NL (1) NL7708669A (en)
SE (1) SE433090B (en)
TR (1) TR19513A (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4299848A (en) 1979-06-08 1981-11-10 International Telephone And Telegraph Corporation Modified enzyme system to inhibit bread firming method for preparing same and use of same in bread and other bakery products
US4329429A (en) * 1980-03-24 1982-05-11 Pfizer Inc. Lactase preparation
GB9703641D0 (en) * 1997-02-21 1997-04-09 Cerestar Holding Bv Purified acid stable alpha-amylase from fungal origin
JP6119025B1 (en) * 2016-06-03 2017-04-26 啓雄 水井 Manufacturing method of mirin

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU269889A1 (en) * Украинский научно исследовательский институт пищевой METHOD OF OBTAINING AMYLOLYTIC ENZYME PREPARATIONS
US1634310A (en) * 1922-07-25 1927-07-05 Jr Jokichi Takamine Process of making alpha yeast stimulant
GB315877A (en) * 1928-07-21 1930-12-18 Albert Rene Boidin Method of purification of solutions of enzymes for use in various industries and the like
US2683682A (en) * 1950-02-06 1954-07-13 Research Corp Differential inactivation of enzymes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3249512A (en) * 1963-05-28 1966-05-03 Harold E Bode In situ dextrose production in crude amylaceous materials
US3524798A (en) * 1967-05-23 1970-08-18 Standard Brands Inc Stabilization of alpha-amylase preparations
CA940070A (en) * 1968-12-23 1974-01-15 Jim S. Berry Stabilized aqueous enzyme composition

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU269889A1 (en) * Украинский научно исследовательский институт пищевой METHOD OF OBTAINING AMYLOLYTIC ENZYME PREPARATIONS
US1634310A (en) * 1922-07-25 1927-07-05 Jr Jokichi Takamine Process of making alpha yeast stimulant
GB315877A (en) * 1928-07-21 1930-12-18 Albert Rene Boidin Method of purification of solutions of enzymes for use in various industries and the like
US2683682A (en) * 1950-02-06 1954-07-13 Research Corp Differential inactivation of enzymes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
EXBK/71 *

Also Published As

Publication number Publication date
DK350177A (en) 1978-02-06
AU515262B2 (en) 1981-03-26
IT1085406B (en) 1985-05-28
JPS5734798B2 (en) 1982-07-24
SE433090B (en) 1984-05-07
ES461346A1 (en) 1978-12-01
FR2368497B1 (en) 1982-12-17
SE7708917L (en) 1978-02-06
DE2735480A1 (en) 1978-02-09
AU2757877A (en) 1979-02-08
JPS5344688A (en) 1978-04-21
TR19513A (en) 1979-03-29
DE2735480C2 (en) 1986-11-13
AR219710A1 (en) 1980-09-15
GB1546556A (en) 1979-05-23
NL7708669A (en) 1978-02-07
BR7705171A (en) 1978-04-25

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