FI953792A0 - Immobilisoidut proteiinit, joilla on spesifiset sitoutumiskyvyt, ja niiden käyttö menetelmissä ja tuotteissa - Google Patents

Immobilisoidut proteiinit, joilla on spesifiset sitoutumiskyvyt, ja niiden käyttö menetelmissä ja tuotteissa

Info

Publication number
FI953792A0
FI953792A0 FI953792A FI953792A FI953792A0 FI 953792 A0 FI953792 A0 FI 953792A0 FI 953792 A FI953792 A FI 953792A FI 953792 A FI953792 A FI 953792A FI 953792 A0 FI953792 A0 FI 953792A0
Authority
FI
Finland
Prior art keywords
protein
alpha
pct
binding protein
yeast
Prior art date
Application number
FI953792A
Other languages
English (en)
Swedish (sv)
Other versions
FI953792A (fi
Inventor
Leon Gerardus J Frenken
Geus Pieter De
Franciscus Maria Klis
Holger York Toschka
Cornelis Theodorus Verrips
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Nv filed Critical Unilever Nv
Publication of FI953792A publication Critical patent/FI953792A/fi
Publication of FI953792A0 publication Critical patent/FI953792A0/fi

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16Enzymes or microbial cells immobilised on or in a biological cell
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
FI953792A 1993-02-10 1995-08-10 Immobilisoidut proteiinit, joilla on spesifiset sitoutumiskyvyt, ja niiden käyttö menetelmissä ja tuotteissa FI953792A0 (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93200350 1993-02-10
PCT/EP1994/000427 WO1994018330A1 (en) 1993-02-10 1994-02-10 Immobilized proteins with specific binding capacities and their use in processes and products

Publications (2)

Publication Number Publication Date
FI953792A FI953792A (fi) 1995-08-10
FI953792A0 true FI953792A0 (fi) 1995-08-10

Family

ID=8213623

Family Applications (1)

Application Number Title Priority Date Filing Date
FI953792A FI953792A0 (fi) 1993-02-10 1995-08-10 Immobilisoidut proteiinit, joilla on spesifiset sitoutumiskyvyt, ja niiden käyttö menetelmissä ja tuotteissa

Country Status (12)

Country Link
US (1) US6114147A (fi)
EP (1) EP0682710B1 (fi)
JP (1) JP3691055B2 (fi)
AT (1) ATE253118T1 (fi)
AU (1) AU6139794A (fi)
DE (1) DE69433279T2 (fi)
DK (1) DK0682710T3 (fi)
ES (1) ES2210248T3 (fi)
FI (1) FI953792A0 (fi)
PT (1) PT682710E (fi)
WO (1) WO1994018330A1 (fi)
ZA (1) ZA94916B (fi)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
CN1070533C (zh) * 1993-02-26 2001-09-05 三宝乐啤酒株式会社 酵母凝集基因及含这些基因的酵母
US5766914A (en) * 1995-01-26 1998-06-16 Michigan State University Method of producing and purifying enzymes
EP1019435A4 (en) * 1996-01-29 2001-12-12 Univ Georgetown AMPLIFICATION OF THE RESPONSE OF AN EXPRESSED RECOMBINANT PROTEIN
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6696251B1 (en) * 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
EP0879878A1 (en) * 1997-05-23 1998-11-25 Quest International B.V. Beer and similar light-sensitive beverages with increased flavour stability and process for producing them
EP0916726A1 (en) 1997-11-13 1999-05-19 Rijksuniversiteit te Groningen Attaching substances to micro-organisms
US6759243B2 (en) 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
ATE535154T1 (de) 1998-03-12 2011-12-15 Vhsquared Ltd Produkten die inaktivierte hefen oder schimmel enthalten, die auf ihrer aussenoberfläche aktive antikörper haben
EP0954978B1 (en) 1998-03-12 2011-11-30 VHsquared Limited New products comprising inactivated yeasts or moulds provided with active antibodies
US7060669B1 (en) 1999-03-25 2006-06-13 Valtion Teknillinen Tutkimuskeskus Process for partitioning of proteins
CN100365125C (zh) * 2000-07-26 2008-01-30 韩国生命工学研究院 来源于酵母的新型细胞壁锚定蛋白及其基因和使用其的细胞表面表达***
US20020165149A1 (en) * 2000-12-08 2002-11-07 Kranz David M. Mutated class II major histocompatibility proteins
AU2002229639A1 (en) * 2000-12-13 2002-06-24 De Haard, Johannes Joseph Wilhelmus Camelidae antibody arrays
CN1708510B (zh) * 2001-04-19 2012-08-15 关西化学机械制作株式会社 细胞表层结合性蛋白质及其应用
DE60232071D1 (de) 2001-06-11 2009-06-04 Applied Nanosystems Bv Verfahren zur bindung von acma-typ protein anker fusionen an mikroorganismen zellwandmaterialien
EP1438400B1 (en) 2001-10-01 2009-06-17 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
US7297532B2 (en) * 2002-01-25 2007-11-20 The Board Of Trustees Of The Leland Stanford Junior University Surface based translation system
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US8044180B2 (en) * 2002-08-14 2011-10-25 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US8968730B2 (en) 2002-08-14 2015-03-03 Macrogenics Inc. FcγRIIB specific antibodies and methods of use thereof
US8187593B2 (en) * 2002-08-14 2012-05-29 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
DK1534335T4 (en) 2002-08-14 2015-10-05 Macrogenics Inc FCGAMMARIIB-SPECIFIC ANTIBODIES AND PROCEDURES FOR USE THEREOF
US8530627B2 (en) 2002-08-14 2013-09-10 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US8946387B2 (en) * 2002-08-14 2015-02-03 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US8193318B2 (en) * 2002-08-14 2012-06-05 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7355008B2 (en) 2003-01-09 2008-04-08 Macrogenics, Inc. Identification and engineering of antibodies with variant Fc regions and methods of using same
US7960512B2 (en) 2003-01-09 2011-06-14 Macrogenics, Inc. Identification and engineering of antibodies with variant Fc regions and methods of using same
NZ547279A (en) 2003-10-22 2008-04-30 Keck Graduate Inst Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
JP5236189B2 (ja) 2003-12-05 2013-07-17 チルドレンズ ホスピタル メディカル センター オリゴ糖組成物および感染症の治療における該組成物の使用
WO2005110474A2 (en) * 2004-05-10 2005-11-24 Macrogenics, Inc. HUMANIZED FcϜRIIB SPECIFIC ANTIBODIES AND METHODS OF USE THEREOF
US7632497B2 (en) 2004-11-10 2009-12-15 Macrogenics, Inc. Engineering Fc Antibody regions to confer effector function
EP1674479A1 (en) * 2004-12-22 2006-06-28 Memorial Sloan-Kettering Cancer Center Modulation of Fc Gamma receptors for optimizing immunotherapy
US9963510B2 (en) * 2005-04-15 2018-05-08 Macrogenics, Inc. Covalent diabodies and uses thereof
US9889197B2 (en) 2005-04-15 2018-02-13 Macrogenics, Inc. Covalently-associated diabody complexes that possess charged coil domains and that are capable of enhanced binding to serum albumin
ES2707152T3 (es) 2005-04-15 2019-04-02 Macrogenics Inc Diacuerpos covalentes y usos de los mismos
US9284375B2 (en) 2005-04-15 2016-03-15 Macrogenics, Inc. Covalent diabodies and uses thereof
US11254748B2 (en) 2005-04-15 2022-02-22 Macrogenics, Inc. Covalent diabodies and uses thereof
EP1726650A1 (en) * 2005-05-27 2006-11-29 Universitätsklinikum Freiburg Monoclonal antibodies and single chain antibody fragments against cell-surface prostate specific membrane antigen
CA2610931A1 (en) * 2005-06-17 2006-12-28 Biorexis Pharmaceutical Corporation Anchored transferrin fusion protein libraries
JP2009501522A (ja) * 2005-07-12 2009-01-22 コドン デバイシズ インコーポレイテッド 生体触媒工学のための組成物及び方法
EP1746103A1 (en) * 2005-07-20 2007-01-24 Applied NanoSystems B.V. Bifunctional protein anchors
SI1919503T1 (sl) * 2005-08-10 2015-02-27 Macrogenics, Inc. Identifikacija in inĺ˝eniring protiteles z variantnimi fc regijami in postopki za njih uporabo
AU2007226752A1 (en) * 2006-03-10 2007-09-20 Macrogenics, Inc. Identification and engineering of antibodies with variant heavy chains and methods of using same
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
EP1918302A3 (en) * 2006-05-18 2009-11-18 AvantGen, Inc. Methods for the identification and the isolation of epitope specific antibodies
CA2660592C (en) 2006-05-26 2016-07-12 Macrogenics, Inc. Humanized fc.gamma.riib-specific antibodies and methods of use thereof
EP2032159B1 (en) * 2006-06-26 2015-01-07 MacroGenics, Inc. Combination of fcgammariib antibodies and cd20-specific antibodies and methods of use thereof
LT2029173T (lt) 2006-06-26 2016-11-10 Macrogenics, Inc. Fc riib specifiniai antikūnai ir jų panaudojimo būdai
US20080112961A1 (en) * 2006-10-09 2008-05-15 Macrogenics, Inc. Identification and Engineering of Antibodies with Variant Fc Regions and Methods of Using Same
US8652466B2 (en) 2006-12-08 2014-02-18 Macrogenics, Inc. Methods for the treatment of disease using immunoglobulins having Fc regions with altered affinities for FcγRactivating and FcγRinhibiting
EP2114999A2 (en) 2006-12-12 2009-11-11 Biorexis Pharmaceutical Corporation Transferrin fusion protein libraries
US20110076752A1 (en) * 2007-02-09 2011-03-31 Medimmune, Llc Antibody library display by yeast cell plasma membrane
WO2008118476A2 (en) 2007-03-26 2008-10-02 Codon Devices, Inc. Cell surface display, screening and production of proteins of interest
CA3187687A1 (en) 2007-09-14 2009-03-19 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
US8420081B2 (en) 2007-11-30 2013-04-16 Abbvie, Inc. Antibody formulations and methods of making same
US8883146B2 (en) 2007-11-30 2014-11-11 Abbvie Inc. Protein formulations and methods of making same
WO2009117030A2 (en) 2007-12-19 2009-09-24 Macrogenics, Inc. Improved compositions for the prevention and treatment of smallpox
JP5731827B2 (ja) 2008-03-03 2015-06-10 グライコフィ, インコーポレイテッド 下等真核生物中での組換えタンパク質の表面ディスプレイ
ITUD20080055A1 (it) * 2008-03-13 2009-09-14 Transactiva S R L Procedimento per la produzione di una proteina umana in pianta, in particolare un enzima lisosomiale umano ricombinante in endosperma di cereali
BRPI0906309A2 (pt) * 2008-04-02 2020-05-26 Macrogenics, Inc Imunoglobulina, anticorpo, uso do anticorpo e composição farmacêutica
CN106349390B (zh) 2008-04-02 2019-12-10 宏观基因有限公司 Bcr-复合体-特异性抗体和其使用方法
US20110081347A1 (en) * 2008-06-04 2011-04-07 Macrogenics, Inc. Antibodies with Altered Binding to FcRn and Methods of Using Same
US8067339B2 (en) 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
JP5985826B2 (ja) 2008-12-16 2016-09-06 ノバルティス アーゲー 酵母ディスプレイ系
CA2776385C (en) 2009-10-07 2019-04-09 Macrogenics, Inc. Fc region-containing polypeptides that exhibit improved effector function due to alterations of the extent of fucosylation, and methods for their use
US8802091B2 (en) 2010-03-04 2014-08-12 Macrogenics, Inc. Antibodies reactive with B7-H3 and uses thereof
PH12018501083A1 (en) 2010-03-04 2019-02-18 Macrogenics Inc Antibodies reactive with b7-h3, immunologically active fragments thereof and uses thereof
DK2593594T3 (en) 2010-07-16 2017-12-11 Adimab Llc ANTIBODY LIBRARIES
WO2012105833A1 (en) 2011-02-01 2012-08-09 Bac Ip B.V. Antigen-binding protein directed against epitope in the ch1 domain of human igg antibodies
EP2710382B1 (en) 2011-05-17 2017-10-18 Bristol-Myers Squibb Company Improved methods for the selection of binding proteins
EA201391753A1 (ru) 2011-05-21 2014-08-29 Макродженикс, Инк. Домены, связывающиеся с деиммунизованной сывороткой, и их применение для увеличения времени полужизни в сыворотке
BR112014009799A2 (pt) 2011-10-24 2017-06-13 Abbvie Inc imunoligantes dirigidos conra tnf
US9487587B2 (en) 2013-03-05 2016-11-08 Macrogenics, Inc. Bispecific molecules that are immunoreactive with immune effector cells of a companion animal that express an activating receptor and cells that express B7-H3 and uses thereof
AU2014244286B2 (en) 2013-03-14 2018-11-08 Duke University Bispecific molecules that are immunoreactive with immune effector cells that express an activating receptor
US11384149B2 (en) 2013-08-09 2022-07-12 Macrogenics, Inc. Bi-specific monovalent Fc diabodies that are capable of binding CD32B and CD79b and uses thereof
UA116479C2 (uk) 2013-08-09 2018-03-26 Макродженікс, Інк. БІСПЕЦИФІЧНЕ МОНОВАЛЕНТНЕ Fc-ДІАТІЛО, ЯКЕ ОДНОЧАСНО ЗВ'ЯЗУЄ CD32B I CD79b, ТА ЙОГО ЗАСТОСУВАННЯ
EP2839842A1 (en) 2013-08-23 2015-02-25 MacroGenics, Inc. Bi-specific monovalent diabodies that are capable of binding CD123 and CD3 and uses thereof
EP2840091A1 (en) 2013-08-23 2015-02-25 MacroGenics, Inc. Bi-specific diabodies that are capable of binding gpA33 and CD3 and uses thereof
AU2015323860B2 (en) 2014-09-29 2021-05-27 Duke University Bispecific molecules comprising an HIV-1 envelope targeting arm
US11136613B2 (en) 2015-04-30 2021-10-05 Board Of Regents, The University Of Texas System Antibacterial polypeptide libraries and methods for screening the same
US10961311B2 (en) 2016-04-15 2021-03-30 Macrogenics, Inc. B7-H3 binding molecules, antibody drug conjugates thereof and methods of use thereof
WO2018104893A1 (en) 2016-12-06 2018-06-14 Glaxosmithkline Intellectual Property Development Limited Alpha4-beta7 antibodies with incrased fcrn binding and/or half-life

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4859609A (en) * 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
WO1992004363A1 (en) * 1990-09-04 1992-03-19 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0474891A1 (en) * 1990-09-08 1992-03-18 BEHRINGWERKE Aktiengesellschaft Vectors for expression of malarial antigens on the surface of Salmonella vaccine strains
SE9101433D0 (sv) * 1991-05-13 1991-05-13 Marianne Hansson Recombinant dna sequence and its use
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US6027910A (en) * 1992-07-08 2000-02-22 Unilever Patent Holdings B.V. Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein
US5618290A (en) * 1993-10-19 1997-04-08 W.L. Gore & Associates, Inc. Endoscopic suture passer and method

Also Published As

Publication number Publication date
DK0682710T3 (da) 2004-03-01
DE69433279T2 (de) 2004-07-15
PT682710E (pt) 2004-03-31
FI953792A (fi) 1995-08-10
WO1994018330A1 (en) 1994-08-18
DE69433279D1 (de) 2003-12-04
ES2210248T3 (es) 2004-07-01
US6114147A (en) 2000-09-05
JP3691055B2 (ja) 2005-08-31
EP0682710A1 (en) 1995-11-22
AU6139794A (en) 1994-08-29
ZA94916B (en) 1995-08-10
JPH08506484A (ja) 1996-07-16
EP0682710B1 (en) 2003-10-29
ATE253118T1 (de) 2003-11-15

Similar Documents

Publication Publication Date Title
FI953792A0 (fi) Immobilisoidut proteiinit, joilla on spesifiset sitoutumiskyvyt, ja niiden käyttö menetelmissä ja tuotteissa
FI950042A (fi) Menetelmä entsyymin immobilisoimiseksi mikrobisolun soluseinämään valmistamalla fuusioproteiini
Hube et al. Sequence of the Candida albicans gene encoding the secretory aspartate proteinase
Salamitou et al. Recognition specificity of the duplicated segments present in Clostridium thermocellum endoglucanase CelD and in the cellulosome-integrating protein CipA
CN1160462C (zh) 利用革兰氏阳性菌表达重组蛋白质
CA2452029C (en) A group of .alpha.-amylases and a method for identification and production of .alpha.-amylases
EP1092764B1 (en) Continuous fermentation
Chambers et al. An exo-β-(1, 3)-glucanase of Candida albicans: purification of the enzyme and molecular cloning of the gene
DE69534822D1 (de) Verfahren zur bakteriellen herstellung von heterologen polypeptiden mit disulfidbrücke
Rothe et al. Cloning, sequencing and expression of a sialidase gene from Clostridium sordellii G12
Miksch et al. The kil gene of the ColE1 plasmid of Escherichia coli controlled by a growth-phase-dependent promoter mediates the secretion of a heterologous periplasmic protein during the stationary phase
CN101638660A (zh) 嗜酸乳杆菌s-层蛋白表面展示***的构建
CN111808834B (zh) 在枯草芽孢杆菌中高效表达耐高温α-淀粉酶的方法、重组启动子以及应用
CA2162022A1 (en) Processes for producing an enzyme
Sharma et al. Expression of functional Porphyromonas gingivalis fimbrillin polypeptide domains on the surface of Streptococcus gordonii
Ladds et al. Sxa2 is a serine carboxypeptidase that degrades extracellular P‐factor in the fission yeast Schizosaccharomyces pombe
EP1293571A3 (en) Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein
JPH1175879A (ja) プロテインジスルフィドイソメラーゼを含有する融合タンパク質をコードするdna
ES2347398B1 (es) Esterasa termofila de thermus thermophilus.
Uemura et al. mRNA expression of MT1-MMP, MMP-9, cathepsin K, and TRAP in highly enriched osteoclasts cultured on several matrix proteins and ivory surfaces
Ghigo et al. Protease secretion by Erwinia chrysanthemi and Serratia marcescens: A signal peptide independent pathway in Gram-negative bacteria
CN108531498A (zh) 人蛋白基因fkbp52过表达载体及其制备方法和应用
Oshiro et al. An improved method for extraction of mRNA and protein from the fungus Chaetomium gracile with anhydrous hydrogen fluoride
Wolfe et al. Enzymes and Enzyme Systems
Watson Cysteine-rich proteins of Chlamydia.