FI122029B - Endoglukanaser som härstammar från svamp, deras framställning och användning - Google Patents

Endoglukanaser som härstammar från svamp, deras framställning och användning Download PDF

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Publication number
FI122029B
FI122029B FI20086253A FI20086253A FI122029B FI 122029 B FI122029 B FI 122029B FI 20086253 A FI20086253 A FI 20086253A FI 20086253 A FI20086253 A FI 20086253A FI 122029 B FI122029 B FI 122029B
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FI
Finland
Prior art keywords
dsm
identity
seq
cel45a
endoglucanase
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FI20086253A
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English (en)
Finnish (fi)
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FI20086253A0 (sv
FI20086253A (sv
Inventor
Jari Vehmaanperae
Leena Valtakari
Jarno Kallio
George Szakacs
Pentti Ojapalo
Marika Alapuranen
Terhi Puranen
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Ab Enzymes Oy
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Publication date
Application filed by Ab Enzymes Oy filed Critical Ab Enzymes Oy
Publication of FI20086253A0 publication Critical patent/FI20086253A0/sv
Priority to FI20086253A priority Critical patent/FI122029B/sv
Priority to US13/142,609 priority patent/US8609387B2/en
Priority to DK09836130.6T priority patent/DK2370573T3/en
Priority to MX2011007142A priority patent/MX2011007142A/es
Priority to EP09836130.6A priority patent/EP2370573B1/en
Priority to CN200980153489.3A priority patent/CN102272297B/zh
Priority to BRPI0923915A priority patent/BRPI0923915B1/pt
Priority to PT98361306T priority patent/PT2370573E/pt
Priority to PCT/FI2009/051043 priority patent/WO2010076388A1/en
Priority to ES09836130.6T priority patent/ES2554652T3/es
Publication of FI20086253A publication Critical patent/FI20086253A/sv
Application granted granted Critical
Publication of FI122029B publication Critical patent/FI122029B/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Textile Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (18)

1. Endoglukanaspolypeptid som härstammar frän svamp, och som hör till glykosylhydrolasfamilj 45, och som har en väsentlig effekt vid läga tem- 5 peraturer, och som omfattar en aminosyrasekvens, som har minst 65-procentig identitet med sekvens nr 13, minst 48-procentig identitet med sekvens nr 15, minst 87-procentig identitet med sekvens nr 17, minst 90-procentig identitet med sekvens nr 19, minst 90-procentig identitet med sekvens nr 21 eller minst 49-procentig identitet med sekvens nr 23, eller ett enzymatiskt aktivt fragment 10 därav.
2. Endoglukanaspolypeptid enligt patentkrav 1, vilken omfattar en aminosyrasekvens, som har minst 90-procentig identitet, företrädesvis minst 95-proecntig identitet och heist minst 98-procentig identitet med sekvens nr 13, 15, 17 eller 23, eller ett enzymatiskt aktivt fragment därav.
3. Endoglukanaspolypeptid enligt patentkrav 1, vilken omfattar en aminosyrasekvens, som har minst 95-procentig identitet, och företrädesvis minst 98-proecntig identitet med sekvens nr 19 eller 21, eller ett enzymatiskt aktivt fragment därav.
4. Endoglucanaspolypeptid enligt patentkrav 1, vilken härstammar 20 frän Geomyces eller Fusarium, företrädesvis frän G. pannorum eller F. cfequi- seti, heist frän G. pannorum RF 6293 (CBS 119567), F. cf. equiseti RF6318 (CBS 119568), G. pannorum RF6546 (CBS 119958), eller G. pannorum RF6608 (CBS 119962).
5. Isolerad polynukleotid, som väljs ur gruppen som bestär av: 25 a) en nukleotidsekvens med en sekvens enligt sekvens nr 12, 14, 16, 18, 20 eller 22, eller en sekvens som kodar en endoglukanaspolypeptid en- 5 ligt patentkrav 1, CM ^ b) en sträng som är komplementär med en sträng enligt a), eller ° c) en sträng som är degenererad i avseende pä den genetiska ko- ^ 30 den av vilken som heist sekvens enligt a) eller b).
6. Polynukleotid enligt patentkrav 5, vilken motsvarar en polynuk- oo leotid som finns i E. co//-stammen DSM 18916, DSM 19171, DSM 19173, DSM 18915, DSM 18917, eller DSM 19170.
7. Expressionsvektor, vilken innehäller en polynukleotid enligt pa- ^ 35 tentkrav 5.
8. Värdcell, vilken innehäller en expressionsvektor enligt patent- krav 7.
9. Förfarande för framställning av en endoglukanaspolypeptid enligt patentkrav 1, vilket innebär att man transformerar en värdcell med en express- 5 ionsvektor, som kodar nämnda polypeptid, och odlar nämnda värdcell under betingelser som möjliggör expression av nämnda polypeptid, och eventuellt tar tillvara polypeptiden och renarden.
10. Enzympreparat, vilket innehäller en endoglukanaspolypeptid enligt patentkrav 1. 10
11. Förfarande för behandling av cellulosahaltigt material, vilket om- fattar att man bringar det cellulosahaltiga materialet i kontakt med en endoglukanaspolypeptid enligt patentkrav 1 eller med ett enzympreparat enligt patentkrav 10.
12. Förfarande enligt patentkrav 11, där behandlingen utförs vid en 15 temperatur pä <50 °C, företrädesvis <40 °C.
13. Förfarande enligt patentkrav 11, vilket utförs vid ett pH pä ca 3-9, företrädesvis 4-8 och heist 5-6,5.
14. Förfarande enligt patentkrav 11, vilket är biostentvätt eller bio- finishing. 20
15. Förfarande enligt patentkrav 11, vilket innebär hydrolys av lignocellulosahaltigt material eller livsmedelsapplikation.
16. Tvättmedelskomposition, vilken omfattar en endoglukanaspolypeptid enligt patentkrav 1 eller ett enzympreparat enligt patentkrav 10.
17. Djurfoder, vilket omfattar en endoglukanaspolypeptid enligt pa-25 tentkrav 1 eller ett enzympreparat enligt patentkrav 10.
18. E. coli-stam, vilken har deponeringsnummer DSM 18916, DSM 5 19171, DSM 19173, DSM 18915, DSM 18917, eller DSM 19170. CM ιό O 00 X cc CL oo m (M co oo o o (M
FI20086253A 2008-12-30 2008-12-30 Endoglukanaser som härstammar från svamp, deras framställning och användning FI122029B (sv)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FI20086253A FI122029B (sv) 2008-12-30 2008-12-30 Endoglukanaser som härstammar från svamp, deras framställning och användning
EP09836130.6A EP2370573B1 (en) 2008-12-30 2009-12-28 Fungal endoglucanases, their production and use
DK09836130.6T DK2370573T3 (en) 2008-12-30 2009-12-28 Svampeendoglucanaser, their production and use
MX2011007142A MX2011007142A (es) 2008-12-30 2009-12-28 Endonucleasas fungicas, su produccion y uso.
US13/142,609 US8609387B2 (en) 2008-12-30 2009-12-28 Fungal endoglucanases, their production and use
CN200980153489.3A CN102272297B (zh) 2008-12-30 2009-12-28 真菌内切葡聚糖酶、它们的生产和应用
BRPI0923915A BRPI0923915B1 (pt) 2008-12-30 2009-12-28 endoglucanases fúngicas, seu método de produção, processo para tratar material celulósico, composição detergente,alimento animal, cepa de e. coli, vetor de expressão, e microrganismo transgênico
PT98361306T PT2370573E (pt) 2008-12-30 2009-12-28 Endoglucanases fúngicas, sua produção e utilização.
PCT/FI2009/051043 WO2010076388A1 (en) 2008-12-30 2009-12-28 Fungal endoglucanases, their production and use
ES09836130.6T ES2554652T3 (es) 2008-12-30 2009-12-28 Endoglucanasas fúngicas, su producción y su uso

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20086253A FI122029B (sv) 2008-12-30 2008-12-30 Endoglukanaser som härstammar från svamp, deras framställning och användning
FI20086253 2008-12-30

Publications (3)

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FI20086253A0 FI20086253A0 (sv) 2008-12-30
FI20086253A FI20086253A (sv) 2010-07-01
FI122029B true FI122029B (sv) 2011-07-29

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FI20086253A FI122029B (sv) 2008-12-30 2008-12-30 Endoglukanaser som härstammar från svamp, deras framställning och användning

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US (1) US8609387B2 (sv)
EP (1) EP2370573B1 (sv)
CN (1) CN102272297B (sv)
BR (1) BRPI0923915B1 (sv)
DK (1) DK2370573T3 (sv)
ES (1) ES2554652T3 (sv)
FI (1) FI122029B (sv)
MX (1) MX2011007142A (sv)
PT (1) PT2370573E (sv)
WO (1) WO2010076388A1 (sv)

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BR112013020264A2 (pt) * 2011-02-09 2016-09-20 Novozymes As composição de eliminação de boboto, uso da composição de eliminação de borboto, e, micróbio geneticamente modificado
CN104769094B (zh) * 2012-06-08 2018-03-09 宝洁公司 尤其可与粉末或颗粒组合物组合使用的具有提高的水溶解度的视觉反差美观颗粒
CN102787076B (zh) * 2012-06-18 2014-06-04 中国科学院微生物研究所 一株耐冷玫红假裸囊菌及其在制备冷水纤维素酶中的应用
WO2014026630A1 (en) 2012-08-16 2014-02-20 Novozymes A/S Method for treating textile with endoglucanase
ES2693461T3 (es) 2012-08-16 2018-12-11 Bangladesh Jute Research Institute Enzimas que degradan celulosa y/o hemicelulosas de Macrophomina phaseolina y usos de las mismas
US9328456B2 (en) 2012-08-16 2016-05-03 Novozymes A/S Method for treating textile with endoglucanase
WO2014094618A1 (en) * 2012-12-19 2014-06-26 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2014101753A1 (en) 2012-12-24 2014-07-03 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
US9506050B2 (en) 2012-12-24 2016-11-29 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
US8778639B1 (en) * 2013-02-12 2014-07-15 Novozymes Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
US8771993B1 (en) * 2013-02-12 2014-07-08 Novozymes A/S Polypeptides having endoglucanse activity and polynucleotides encoding same
SG11201601344PA (en) * 2013-10-25 2016-05-30 Novozymes As Polypeptides having endoglucanase activity and polynucleotides encoding same
CN103589701B (zh) * 2013-11-18 2015-02-04 青岛蔚蓝生物集团有限公司 一种低温纤维素酶及其应用
FR3013731B1 (fr) 2013-11-22 2017-09-01 Ifp Energies Now Variants d'endoglucanases a activite amelioree et leurs utilisations
FR3022558B1 (fr) 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
FR3022557B1 (fr) 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
KR20170100495A (ko) 2014-12-31 2017-09-04 노보자임스 에이/에스 폴리에스테르 직물의 처리 방법
WO2016206621A1 (en) 2015-06-26 2016-12-29 Novozymes A/S Biofinishing system
CN110484462B (zh) * 2019-07-09 2021-03-16 中国医学科学院医药生物技术研究所 申氏杆菌属新物种及其应用
CN110643519A (zh) * 2019-10-29 2020-01-03 华东理工大学 ***素高产的南极真菌突变株、其选育方法及其应用
CN111154743B (zh) * 2020-04-01 2021-01-05 黑龙江省农业科学院畜牧兽医分院 突变型纤维素酶及其在玉米青贮中的应用

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Publication number Publication date
EP2370573A1 (en) 2011-10-05
ES2554652T3 (es) 2015-12-22
MX2011007142A (es) 2011-07-28
FI20086253A0 (sv) 2008-12-30
CN102272297B (zh) 2014-07-30
BRPI0923915A2 (pt) 2015-10-06
EP2370573A4 (en) 2012-12-12
EP2370573B1 (en) 2015-10-21
US8609387B2 (en) 2013-12-17
BRPI0923915B1 (pt) 2019-09-10
FI20086253A (sv) 2010-07-01
DK2370573T3 (en) 2015-12-14
WO2010076388A1 (en) 2010-07-08
PT2370573E (pt) 2015-12-24
BRPI0923915A8 (pt) 2017-12-19
CN102272297A (zh) 2011-12-07
US20110269212A1 (en) 2011-11-03

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